Material Safety Data Sheet 95171150015

GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Material uses Industrial applications: Analytical chemistry. Research. Product type Liquid. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Liquid. Color Colorless. Odor Pungent. Apple-like. Signal word DANGER! Hazard statements MAY BE FATAL IF INHALED. CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. HARMFUL IF SWALLOWED. MAY CAUSE ALLERGIC RESPIRATORY AND SKIN REACTION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Precautionary measures Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Do not get in eyes. Do not get on skin. Do not eat, drink or smoke when using this product. Avoid prolonged or repeated contact with skin. Keep container tightly closed. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Corrosive to eyes. Causes burns. Skin Corrosive to the skin. Causes burns. May cause sensitization by skin contact. Inhalation Very toxic by inhalation. Corrosive to the respiratory system. May cause sensitization by inhalation. Ingestion Toxic if swallowed. May cause burns to mouth, throat and stomach. Potential chronic health effects Chronic effects Contains material that can cause target organ damage. Once sensitized, a severe allergic reaction may occur when subsequently exposed to very low levels. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Target organs Contains material which may cause damage to the following organs: heart, upper respiratory tract, skin, central nervous system (CNS), eye, lens or cornea.

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 Inhalation Adverse symptoms may include the following: respiratory tract irritation coughing wheezing and breathing difficulties asthma Ingestion Adverse symptoms may include the following: stomach pains Skin Adverse symptoms may include the following: pain or irritation redness blistering may occur Eyes Adverse symptoms may include the following: pain watering redness Medical conditions aggravated by Pre-existing respiratory and skin disorders and disorders involving any other target organs mentioned in over-exposure this MSDS as being at risk may be aggravated by over-exposure to this product. See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight glutaral 111-30-8 25

There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Section 4. First aid measures Eye contact Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. Skin contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. Inhalation Call medical doctor or poison control center immediately. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Ingestion Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Protection of first-aiders No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. Section 5. Fire-fighting measures

Flammability of the product In a fire or if heated, a pressure increase will occur and the container may burst. Extinguishing media Suitable Use an extinguishing agent suitable for the surrounding fire. Not suitable None known. Special exposure hazards Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Special protective equipment for Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) fire-fighters with a full face-piece operated in positive pressure mode.

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Do not breathe vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Methods for cleaning up Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see Section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor.

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 7. Handling and storage

Recommended monitoring If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological procedures monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference to national guidance documents for methods for the determination of hazardous substances will also be required. Engineering measures Use only with adequate ventilation. Use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles and/or face shield. If inhalation hazards exist, a full-face respirator may be required instead. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels.

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 9. Physical and chemical properties

Physical state Liquid. Color Colorless. Odor Pungent. Apple-like. Boiling/condensation point 100 to 101°C (212 to 213.8°F) Melting/freezing point -6°C (21.2°F) Relative density 1.106 Vapor pressure 2 kPa (15 mm Hg) [room temperature] Vapor density 3.5 [Air = 1] Volatility 0% (v/v) Evaporation rate 0.88 (butyl acetate = 1) Solubility Easily soluble in the following materials: cold water, hot water and acetone. Section 10. Stability and reactivity

Chemical stability The product is stable. Incompatible materials No specific data. Possibility of hazardous reactions Under normal conditions of storage and use, hazardous reactions will not occur. Section 11. Toxicological information

Acute toxicity Product/ingredient name Result Species Dose Exposure glutaral LC50 Inhalation Vapor Rat 480 mg/m³ 4 hours LD50 Oral Rat 134 mg/kg - Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available. Carcinogenicity Conclusion/Summary Not available. Classification Product/ingredient name OSHA IARC NTP ACGIH EPA NIOSH glutaral - - - A4 - -

Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available.

Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Product/ingredient name Result Species Exposure glutaral Acute EC50 0.75 to 1 ppm Fresh water Daphnia - Daphnia magna 48 hours Acute LC50 5.4 ppm Fresh water Fish - Pimephales promelas 96 hours Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards.

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Not available. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Section 14. Transport information Regulatory information UN number Proper shipping name Classes PG* Label Additional information DOT Classification UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II - (glutaral)

TDG Classification UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II - (glutaral)

Mexico Classification UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II - (glutaral)

ADR/RID Class UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II Tunnel code (glutaral) (D/E)

IMDG Class UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II - (glutaral)

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 IATA-DGR Class UN2927 Toxic liquid, corrosive, organic, n.o.s. 6.1 (8) II - (glutaral)

PG* : Packing group Section 15. Regulatory information HCS Classification Highly toxic material Corrosive material Sensitizing material Target organ effects U.S. Federal regulations TSCA 8(a) PAIR: glutaral TSCA 8(a) CDR Exempt/Partial exemption: Not determined TSCA 8(d) H and S data reporting: glutaral United States inventory (TSCA 8b): All components are listed or exempted. Clean Air Act Section 112(b) Not listed Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

No products were found.

SARA 304 RQ Not applicable. SARA 311/312 Classification Immediate (acute) health hazard Delayed (chronic) health hazard Composition/information on ingredients Name % Fire Sudden Reactive Immediate Delayed hazard release of (acute) health (chronic) pressure hazard health hazard glutaral 25 No. No. No. Yes. Yes.

State regulations Massachusetts The following components are listed: GLUTARALDEHYDE New York None of the components are listed. New Jersey The following components are listed: GLUTARALDEHYDE; PENTANEDIAL Pennsylvania The following components are listed: PENTANEDIAL California Prop. 65 United States inventory (TSCA 8b) All components are listed or exempted. International regulations

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 Glutaraldehyde, 25%; part of 'Silver Staining Kit, Protein' 17-1150-01 International lists Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Japan inventory: All components are listed or exempted. Korea inventory: All components are listed or exempted. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements MAY BE FATAL IF INHALED. CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. HARMFUL IF SWALLOWED. MAY CAUSE ALLERGIC RESPIRATORY AND SKIN REACTION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. The customer is responsible for determining the PPE code for this material.

0 Flammability National Fire Protection Association (U.S.A.) Health 3 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6.1 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

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17115001-5 Validation date 21 October 2013 95171150015 Version 6.1 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Sodium Thiosulphate 5%; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Hazard statements CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. Precautionary measures Do not breathe vapor or mist. Do not eat, drink or smoke when using this product. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes No known significant effects or critical hazards. Skin No known significant effects or critical hazards. Inhalation No known significant effects or critical hazards. Ingestion No known significant effects or critical hazards. Potential chronic health effects Chronic effects Contains material that may cause target organ damage, based on animal data. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Target organs Contains material which may cause damage to the following organs: upper respiratory tract.

Inhalation No specific data. Ingestion No specific data. Skin No specific data. Eyes No specific data. Medical conditions aggravated by Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be over-exposure aggravated by over-exposure to this product. See toxicological information (Section 11)

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17115001-2 Validation date 21 October 2013 95171150012 Version 6 Sodium Thiosulphate 5%; part of 'Silver Staining Kit, Protein' 17-1150-01 3. Composition/information on ingredients Name CAS number % by weight sodium thiosulphate 7772-98-7 5

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Methods for cleaning up Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see Section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Section 7. Handling and storage

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17115001-2 Validation date 21 October 2013 95171150012 Version 6 Sodium Thiosulphate 5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory A respirator is not needed under normal and intended conditions of product use. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: safety glasses with side-shields. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Liquid. Color Colorless. Odor Odorless. Volatility 0% (v/v) Solubility Easily soluble in the following materials: cold water and hot water. Section 10. Stability and reactivity

Acute toxicity Product/ingredient name Result Species Dose Exposure sodium thiosulphate LD50 Oral Rat >5000 mg/kg -

Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available. Carcinogenicity Conclusion/Summary Not available. Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available.

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17115001-2 Validation date 21 October 2013 95171150012 Version 6 Sodium Thiosulphate 5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Product/ingredient name Result Species Exposure sodium thiosulphate Acute LC50 24000000 µg/l Fresh water Fish - Gambusia affinis - Adult 96 hours Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Not classified. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Section 14. Transport information IATA-DGR Class Classes PG*

PG* : Packing group Section 15. Regulatory information HCS Classification Target organ effects U.S. Federal regulations TSCA 8(a) CDR Exempt/Partial exemption: Not determined United States inventory (TSCA 8b): All components are listed or exempted. Clean Air Act Section 112(b) Not listed Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

No products were found.

SARA 304 RQ Not applicable. SARA 311/312 Classification Delayed (chronic) health hazard Composition/information on ingredients Name % Fire Sudden Reactive Immediate Delayed hazard release of (acute) health (chronic) pressure hazard health hazard sodium thiosulphate 5 No. No. No. No. Yes.

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17115001-2 Validation date 21 October 2013 95171150012 Version 6 Sodium Thiosulphate 5%; part of 'Silver Staining Kit, Protein' 17-1150-01

State regulations Massachusetts None of the components are listed. New York None of the components are listed. New Jersey None of the components are listed. Pennsylvania None of the components are listed. California Prop. 65 United States inventory (TSCA 8b) All components are listed or exempted. International regulations International lists Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Japan inventory: All components are listed or exempted. Korea inventory: All components are listed or exempted. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. The customer is responsible for determining the PPE code for this material.

0 Flammability National Fire Protection Association (U.S.A.) Health 0 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

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17115001-2 Validation date 21 October 2013 95171150012 Version 6 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Sodium Acetate; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Synonym Acetic acid, sodium salt; Acetic acid, sodium salt (1:1) Material uses Industrial applications: Analytical chemistry. Research. Product type Powder. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Solid. [Crystalline powder. Hygroscopic.] Color White. Odor Odorless. Signal word CAUTION! Hazard statements MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. Precautionary measures Do not breathe dust. Use only with adequate ventilation. Do not eat, drink or smoke when using this product. Avoid contact with eyes, skin and clothing. Keep container tightly closed. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Moderately irritating to eyes. Skin Moderately irritating to the skin. Inhalation Moderately irritating to the respiratory system. Ingestion No known significant effects or critical hazards. Potential chronic health effects Chronic effects May cause target organ damage, based on animal data. Repeated or prolonged inhalation of dust may lead to chronic respiratory irritation. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Target organs May cause damage to the following organs: skin, eyes.

Inhalation Adverse symptoms may include the following: respiratory tract irritation coughing Ingestion No specific data.

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17115001-1 Validation date 21 October 2013 95171150011 Version 6 Sodium Acetate; part of 'Silver Staining Kit, Protein' 17-1150-01 Skin Adverse symptoms may include the following: irritation redness Eyes Adverse symptoms may include the following: irritation watering redness Medical conditions aggravated by Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be over-exposure aggravated by over-exposure to this product. See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight sodium acetate 127-09-3 100

Flammability of the product No specific fire or explosion hazard. Extinguishing media Suitable Use an extinguishing agent suitable for the surrounding fire. Not suitable None known. Special exposure hazards Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Special protective equipment for Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) fire-fighters with a full face-piece operated in positive pressure mode.

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing dust. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Methods for cleaning up Move containers from spill area. Approach release from upwind. Avoid creating dusty conditions and prevent wind dispersal. Prevent entry into sewers, water courses, basements or confined areas. Avoid dust generation. Do not dry sweep. Vacuum dust with equipment fitted with a HEPA filter and place in a closed, labeled waste container. Dispose of via a licensed waste disposal contractor. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Move containers from spill area. Avoid dust generation. Do not dry sweep. Place spilled material in a designated, labeled waste container. Vacuum dust with equipment fitted with a HEPA filter and place in a closed, labeled waste container. Dispose of via a licensed waste disposal contractor. Section 7. Handling and storage

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17115001-1 Validation date 21 October 2013 95171150011 Version 6 Sodium Acetate; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 8. Exposure controls/personal protection Consult local authorities for acceptable exposure limits. Recommended monitoring If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological procedures monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference to national guidance documents for methods for the determination of hazardous substances will also be required. Engineering measures Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory A respirator is not needed under normal and intended conditions of product use. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles. If operating conditions cause high dust concentrations to be produced, use dust goggles. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Solid. [Crystalline powder. Hygroscopic.] Flash point Closed cup: >250°C (>482°F) [Product does not sustain combustion.] Auto-ignition temperature 607°C (1124.6°F) Color White. Odor Odorless. Molecular weight 82.04

Molecular formula NaC2H3O2 Melting/freezing point 324°C (615.2°F) Vapor pressure 0 kPa (0 mm Hg) [room temperature] Volatility 0% (w/w) Solubility Easily soluble in the following materials: cold water and hot water. Insoluble in the following materials: diethyl ether. Section 10. Stability and reactivity

Chemical stability The product is stable. Conditions to avoid No specific data. Incompatible materials No specific data. Possibility of hazardous reactions Under normal conditions of storage and use, hazardous reactions will not occur. Section 11. Toxicological information

Acute toxicity Product/ingredient name Result Species Dose Exposure sodium acetate LD50 Oral Rat 3530 mg/kg -

Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available.

Article Number Page: 3/5

17115001-1 Validation date 21 October 2013 95171150011 Version 6 Sodium Acetate; part of 'Silver Staining Kit, Protein' 17-1150-01 Sensitizer Conclusion/Summary Not available. Carcinogenicity Conclusion/Summary Not available. Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available. Section 12. Ecological information Environmental effects Readily biodegradable This product shows a low bioaccumulation potential. Aquatic ecotoxicity sodium acetate Acute LC50 >1000 mg/l Fresh water Fish - Lepomis macrochirus 96 hours Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Bioconcentration factor <10 Toxicity of the products of The product itself and its products of degradation are not toxic. biodegradation Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Not classified. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees.

Section 14. Transport information IATA-DGR Class Classes PG*

PG* : Packing group Section 15. Regulatory information HCS Classification Irritating material Target organ effects U.S. Federal regulations TSCA 8(a) CDR Exempt/Partial exemption: Not determined United States inventory (TSCA 8b): Not determined. Clean Air Act Section 112(b) Not listed Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304

Article Number Page: 4/5

17115001-1 Validation date 21 October 2013 95171150011 Version 6 Sodium Acetate; part of 'Silver Staining Kit, Protein' 17-1150-01 Composition/information on ingredients

No products were found.

State regulations Massachusetts This material is not listed. New York This material is not listed. New Jersey This material is not listed. Pennsylvania This material is not listed. California Prop. 65 United States inventory (TSCA 8b) Not determined. International regulations International lists Australia inventory (AICS): Not determined. China inventory (IECSC): Not determined. Japan inventory: This material is listed or exempted. Korea inventory: Not determined. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): Not determined. Philippines inventory (PICCS): Not determined. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. The customer is responsible for determining the PPE code for this material.

1 Flammability National Fire Protection Association (U.S.A.) Health 1 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Article Number Page: 5/5

17115001-1 Validation date 21 October 2013 95171150011 Version 6 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification

Product name EDTA-Na2; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Synonym Glycine, N,N'-1,2-ethanediylbis[N-(carboxymethyl)-, sodium salt, hydrate (1:2:2); Glycine, N,N'-1, 2-ethanediylbis[N-(carboxymethyl)-, disodium salt, dihydrate; Ethylenediaminetetraacetic acid disodium salt dihydrate; Ethylenediamine tetraacetic acid disodiumsalt dihydrate; Disodium edetate dihydrate; Glycine, N,N'-1,2-ethanedlylbis[N-(carboxymethyl), disodium salt, dihydrate; Disodium dihydrogen ethylenediamine-N,N,N',N'-tetraacetate, dihydrate; EDTA, disodium, dihydrate; Ethylene diamine tetraacetic acid, disodium salt; Disodium EDTA, dihydrate Material uses Industrial applications: Analytical chemistry. Research. Product type Solid. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Solid. [Powder.] Color White. Odor Odorless. Signal word WARNING! Hazard statements CAUSES EYE AND SKIN IRRITATION. MAY BE HARMFUL IF SWALLOWED. Precautionary measures Do not ingest. Avoid contact with eyes, skin and clothing. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Irritating to eyes. Skin Irritating to skin. Inhalation Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure. Ingestion Harmful if swallowed. Potential chronic health effects Chronic effects No known significant effects or critical hazards. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Inhalation No specific data. Ingestion No specific data. Skin Adverse symptoms may include the following: irritation redness

Article Number Page: 1/5

17115001-6 Validation date 21 October 2013 95171150016 Version 6 EDTA-Na2; part of 'Silver Staining Kit, Protein' 17-1150-01 Eyes Adverse symptoms may include the following: pain or irritation watering redness Medical conditions aggravated by None known. over-exposure See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight Acetic acid, (ethylenedinitrilo)tetra-, disodium salt, dihydrate 6381-92-6 100

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Methods for cleaning up Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Avoid dust generation. Do not dry sweep. Vacuum dust with equipment fitted with a HEPA filter and place in a closed, labeled waste container. Dispose of via a licensed waste disposal contractor. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Move containers from spill area. Avoid dust generation. Using a vacuum with HEPA filter will reduce dust dispersal. Place spilled material in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor. Section 7. Handling and storage

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17115001-6 Validation date 21 October 2013 95171150016 Version 6 EDTA-Na2; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 8. Exposure controls/personal protection Consult local authorities for acceptable exposure limits. Recommended monitoring If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological procedures monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference to national guidance documents for methods for the determination of hazardous substances will also be required. Engineering measures Good general ventilation should be sufficient to control worker exposure to airborne contaminants. Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory A respirator is not needed under normal and intended conditions of product use. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Solid. [Powder.] Color White. Odor Odorless. Molecular weight 372.24

Molecular formula C10H14N2Na2O8*2H2O pH 4 to 5.5 [Conc. (% w/w): 0.1%] Melting/freezing point 252°C (485.6°F) Volatility 0% (w/w) Solubility Easily soluble in the following materials: cold water and hot water. Section 10. Stability and reactivity

Acute toxicity Product/ingredient name Result Species Dose Exposure Acetic acid, (ethylenedinitrilo)tetra-, LD50 Oral Rat 2000 mg/kg - disodium salt, dihydrate Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available. Carcinogenicity

Article Number Page: 3/5

17115001-6 Validation date 21 October 2013 95171150016 Version 6 EDTA-Na2; part of 'Silver Staining Kit, Protein' 17-1150-01 Conclusion/Summary Not available. Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available. Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Not classified. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Section 14. Transport information IATA-DGR Class Classes PG*

PG* : Packing group Section 15. Regulatory information HCS Classification Irritating material U.S. Federal regulations TSCA 8(a) CDR Exempt/Partial exemption: Not determined United States inventory (TSCA 8b): This material is listed or exempted. Clean Air Act Section 112(b) Not listed Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

No products were found.

SARA 304 RQ Not applicable. SARA 311/312 Classification Immediate (acute) health hazard Composition/information on ingredients

Article Number Page: 4/5

17115001-6 Validation date 21 October 2013 95171150016 Version 6 EDTA-Na2; part of 'Silver Staining Kit, Protein' 17-1150-01 Name % Fire Sudden Reactive Immediate Delayed hazard release of (acute) health (chronic) pressure hazard health hazard Acetic acid, (ethylenedinitrilo)tetra-, disodium salt, 100 No. No. No. Yes. No. dihydrate

State regulations Massachusetts This material is not listed. New York This material is not listed. New Jersey This material is not listed. Pennsylvania This material is not listed. California Prop. 65 United States inventory (TSCA 8b) This material is listed or exempted. International regulations International lists Australia inventory (AICS): This material is listed or exempted. China inventory (IECSC): This material is listed or exempted. Japan inventory: This material is listed or exempted. Korea inventory: This material is listed or exempted. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): This material is listed or exempted. Philippines inventory (PICCS): This material is listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements CAUSES EYE AND SKIN IRRITATION. MAY BE HARMFUL IF SWALLOWED. The customer is responsible for determining the PPE code for this material.

0 Flammability National Fire Protection Association (U.S.A.) Health 2 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Article Number Page: 5/5

17115001-6 Validation date 21 October 2013 95171150016 Version 6 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Material uses Industrial applications: Analytical chemistry. Research. Product type Liquid. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Liquid. Color Colorless. Odor Odorless. Signal word DANGER! Hazard statements CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. MAY BE HARMFUL IF SWALLOWED. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. SUSPECT CANCER HAZARD - CONTAINS MATERIAL WHICH MAY CAUSE CANCER. Precautionary measures Do not handle until all safety precautions have been read and understood. Obtain special instructions before use. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Do not get in eyes. Do not get on skin. Do not eat, drink or smoke when using this product. Keep container tightly closed. Use personal protective equipment as required. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Corrosive to eyes. Causes burns. Skin Corrosive to the skin. Causes burns. Inhalation Corrosive to the respiratory system. Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure. Ingestion Harmful if swallowed. May cause burns to mouth, throat and stomach. Potential chronic health effects Chronic effects Contains material that may cause target organ damage, based on animal data. Carcinogenicity Contains material which may cause cancer. Risk of cancer depends on duration and level of exposure. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Target organs Contains material which may cause damage to the following organs: blood, kidneys, lungs, mucous membranes, upper respiratory tract, skin, eyes, central nervous system (CNS), nose/sinuses.

Inhalation Adverse symptoms may include the following: respiratory tract irritation coughing

Article Number Page: 1/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Ingestion Adverse symptoms may include the following: stomach pains Skin Adverse symptoms may include the following: pain or irritation redness blistering may occur Eyes Adverse symptoms may include the following: pain watering redness Medical conditions aggravated by Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be over-exposure aggravated by over-exposure to this product. See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight silver nitrate 7761-88-8 2.5

There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Section 4. First aid measures Eye contact Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. Skin contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. Inhalation Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Ingestion Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Protection of first-aiders No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. Section 5. Fire-fighting measures

Section 6. Accidental release measures

Article Number Page: 2/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 7. Handling and storage

Handling Put on appropriate personal protective equipment (see Section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. Avoid exposure - obtain special instructions before use. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Storage Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Section 8. Exposure controls/personal protection Ingredient Exposure limits silver nitrate NIOSH REL (United States, 6/2009). Notes: as Ag TWA: 0.01 mg/m³, (as Ag) 10 hours. Form: METAL DUST AND SOLUBLE ACGIH TLV (United States, 3/2012). Notes: as Ag TWA: 0.01 mg/m³, (as Ag) 8 hours. Form: Soluble OSHA PEL (United States, 6/2010). Notes: as Ag TWA: 0.01 mg/m³, (as Ag) 8 hours. OSHA PEL 1989 (United States, 3/1989). Notes: as Ag TWA: 0.01 mg/m³, (as Ag) 8 hours. Recommended monitoring If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological procedures monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference to national guidance documents for methods for the determination of hazardous substances will also be required. Engineering measures Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles and/or face shield. If inhalation hazards exist, a full-face respirator may be required instead. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Liquid. Color Colorless. Odor Odorless. Volatility 0% (v/v) Solubility Easily soluble in the following materials: cold water and hot water.

Article Number Page: 3/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 10. Stability and reactivity

Acute toxicity Product/ingredient name Result Species Dose Exposure silver nitrate LD50 Oral Rat 1173 mg/kg -

Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available. Carcinogenicity Conclusion/Summary Not available. Classification Product/ingredient name OSHA IARC NTP ACGIH EPA NIOSH silver nitrate - 2A - - - -

Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available.

Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Product/ingredient name Result Species Exposure silver nitrate Acute EC50 0.0026 ppm Fresh water Algae - Pseudokirchneriella subcapitata 96 hours Acute EC50 2 ng/L Marine water Crustaceans - Acartia tonsa - Adult 48 hours Acute LC50 0.18 µg/l Fresh water Daphnia - Daphnia magna - Neonate 48 hours Acute LC50 1.4 µg/l Fresh water Fish - Pimephales promelas - Fry 96 hours Chronic IC10 2 µg/l Fresh water Algae - Staurastrum cristatum 4 days Chronic NOEC 0.9 µg/l Fresh water Crustaceans - Hyalella azteca - Adult 21 days Chronic NOEC 1.49 µg/l Fresh water Daphnia - Daphnia magna 21 days Chronic NOEC 0.038 µg/l Fresh water Fish - Pimephales promelas - Egg 32 days Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees.

Article Number Page: 4/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 14. Transport information Regulatory information UN number Proper shipping name Classes PG* Label Additional information DOT Classification UN3082 ENVIRONMENTALLY HAZARDOUS 9 III Reportable quantity SUBSTANCE, LIQUID, N.O.S. (silver nitrate). 40 lbs / 18.16 kg Package sizes shipped in Marine pollutant RQ (silver nitrate) quantities less than the product reportable quantity are not subject to the RQ (reportable quantity) transportation requirements.

TDG Classification UN3082 ENVIRONMENTALLY HAZARDOUS 9 III - SUBSTANCE, LIQUID, N.O.S. (silver nitrate). Marine pollutant

Mexico Classification UN3082 ENVIRONMENTALLY HAZARDOUS 9 III - SUBSTANCE, LIQUID, N.O.S. (silver nitrate). Marine pollutant (silver nitrate)

ADR/RID Class UN3082 ENVIRONMENTALLY HAZARDOUS 9 III Tunnel code SUBSTANCE, LIQUID, N.O.S. (silver nitrate) (E)

IMDG Class UN3082 ENVIRONMENTALLY HAZARDOUS 9 III - SUBSTANCE, LIQUID, N.O.S. (silver nitrate). Marine pollutant (silver nitrate)

IATA-DGR Class UN3082 ENVIRONMENTALLY HAZARDOUS 9 III - SUBSTANCE, LIQUID, N.O.S. (silver nitrate)

PG* : Packing group Section 15. Regulatory information HCS Classification Corrosive material Carcinogen Target organ effects U.S. Federal regulations TSCA 8(a) CDR Exempt/Partial exemption: Not determined United States inventory (TSCA 8b): All components are listed or exempted. Clean Water Act (CWA) 307: silver nitrate Clean Water Act (CWA) 311: silver nitrate Clean Air Act Section 112(b) Not listed Hazardous Air Pollutants (HAPs)

Article Number Page: 5/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

No products were found.

SARA 313 Product name CAS number Concentration Form R - Reporting silver nitrate 7761-88-8 2.5 requirements Supplier notification silver nitrate 7761-88-8 2.5

SARA 313 notifications must not be detached from the MSDS and any copying and redistribution of the MSDS shall include copying and redistribution of the notice attached to copies of the MSDS subsequently redistributed.

State regulations Massachusetts The following components are listed: SILVER NITRATE New York The following components are listed: Silver nitrate New Jersey The following components are listed: SILVER NITRATE; NITRIC ACID SILVER(1+) SALT Pennsylvania The following components are listed: NITRIC ACID SILVER(1+) SALT California Prop. 65 United States inventory (TSCA 8b) All components are listed or exempted. International regulations International lists Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Japan inventory: All components are listed or exempted. Korea inventory: All components are listed or exempted. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Article Number Page: 6/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 Silver Nitrate, 2.5%; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 16. Other information Label requirements CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. MAY BE HARMFUL IF SWALLOWED. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. SUSPECT CANCER HAZARD - CONTAINS MATERIAL WHICH MAY CAUSE CANCER. The customer is responsible for determining the PPE code for this material.

0 Flammability National Fire Protection Association (U.S.A.) Health 3 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Article Number Page: 7/7

17115001-4 Validation date 21 October 2013 95171150014 Version 6 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Formaldehyde 37%; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Material uses Industrial applications: Analytical chemistry. Research. Product type Liquid. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Liquid. Color Colorless. Odor Pungent. Suffocating. Signal word DANGER! Hazard statements COMBUSTIBLE LIQUID AND VAPOR. CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. HARMFUL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED. MAY CAUSE ALLERGIC RESPIRATORY AND SKIN REACTION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. CANCER HAZARD - CONTAINS MATERIAL WHICH CAN CAUSE CANCER. Precautionary measures Do not handle until all safety precautions have been read and understood. Obtain special instructions before use. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Do not get in eyes. Do not get on skin. Do not eat, drink or smoke when using this product. Avoid contact with eyes, skin and clothing. Keep away from heat and flame. Keep container tightly closed. Use personal protective equipment as required. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Corrosive to eyes. Causes burns. Skin Corrosive to the skin. Causes burns. Toxic in contact with skin. May cause sensitization by skin contact. Inhalation Toxic by inhalation. Corrosive to the respiratory system. May cause sensitization by inhalation. Ingestion Toxic if swallowed. May cause burns to mouth, throat and stomach. Potential chronic health effects Chronic effects Contains material that can cause target organ damage. Once sensitized, a severe allergic reaction may occur when subsequently exposed to very low levels. Carcinogenicity Contains material which can cause cancer. Risk of cancer depends on duration and level of exposure. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Target organs Contains material which may cause damage to the following organs: lungs, the nervous system, mucous membranes, upper respiratory tract, skin, central nervous system (CNS), eye, lens or cornea.

Article Number Page: 1/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 Inhalation Adverse symptoms may include the following: respiratory tract irritation coughing wheezing and breathing difficulties asthma Ingestion Adverse symptoms may include the following: stomach pains Skin Adverse symptoms may include the following: pain or irritation redness blistering may occur Eyes Adverse symptoms may include the following: pain watering redness Medical conditions aggravated by Pre-existing respiratory and skin disorders and disorders involving any other target organs mentioned in over-exposure this MSDS as being at risk may be aggravated by over-exposure to this product. See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight Formaldehyde 50-00-0 37

There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Section 4. First aid measures Eye contact Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. Skin contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. Inhalation Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Ingestion Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Protection of first-aiders No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. Section 5. Fire-fighting measures

Flammability of the product Combustible liquid. In a fire or if heated, a pressure increase will occur and the container may burst, with the risk of a subsequent explosion. Extinguishing media Suitable Use dry chemical, CO₂, water spray (fog) or foam. Not suitable Do not use water jet. Special exposure hazards Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Move containers from fire area if this can be done without risk. Use water spray to keep fire-exposed containers cool. Hazardous combustion products Decomposition products may include the following materials: carbon dioxide carbon monoxide Special protective equipment for Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) fire-fighters with a full face-piece operated in positive pressure mode.

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Shut off all ignition sources. No flares, smoking or flames in hazard area. Do not breathe vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air).

Article Number Page: 2/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 Methods for cleaning up Stop leak if without risk. Move containers from spill area. Use spark-proof tools and explosion-proof equipment. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see Section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Stop leak if without risk. Move containers from spill area. Use spark-proof tools and explosion-proof equipment. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Section 7. Handling and storage

Article Number Page: 3/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 Respiratory Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles and/or face shield. If inhalation hazards exist, a full-face respirator may be required instead. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Liquid. Flash point Closed cup: 84°C (183.2°F) Auto-ignition temperature 423.9°C (795°F) Flammable limits Lower: 7% Upper: 73% Color Colorless. Odor Pungent. Suffocating. Taste Burning. Boiling/condensation point 93 to 96°C (199.4 to 204.8°F) Melting/freezing point <15°C (<59°F) Vapor pressure 2.3 kPa (17.2 mm Hg) [room temperature] Vapor density 1 [Air = 1] Evaporation rate >1 (butyl acetate = 1) Solubility Easily soluble in the following materials: cold water, hot water, diethyl ether and acetone. Section 10. Stability and reactivity

Chemical stability The product is stable. Incompatible materials Reactive or incompatible with the following materials: oxidizing materials Possibility of hazardous reactions Under normal conditions of storage and use, hazardous reactions will not occur. Section 11. Toxicological information

Acute toxicity Product/ingredient name Result Species Dose Exposure Formaldehyde LD50 Dermal Rabbit 270 mg/kg - LD50 Oral Rat 100 mg/kg - Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available. Carcinogenicity Conclusion/Summary Not available. Classification Product/ingredient name OSHA IARC NTP ACGIH EPA NIOSH Formaldehyde + 1 Known to be a human carcinogen. A2 B +

Mutagenicity Conclusion/Summary Not available.

Article Number Page: 4/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available.

Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Product/ingredient name Result Species Exposure Formaldehyde Acute EC50 0.788 mg/l Marine water Algae - Ulva pertusa 96 hours Acute EC50 12.98 mg/l Fresh water Crustaceans - Ceriodaphnia dubia - 48 hours Neonate Acute EC50 5800 µg/l Fresh water Daphnia - Daphnia pulex - Neonate 48 hours Acute LC50 1.41 ppm Fresh water Fish - Oncorhynchus mykiss 96 hours Chronic NOEC 0.438 mg/l Marine water Algae - Ulva pertusa 96 hours Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Vapor from product residues may create a highly flammable or explosive atmosphere inside the container. Do not cut, weld or grind used containers unless they have been cleaned thoroughly internally. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Waste stream Code: U122 Classification: Formaldehyde United States - RCRA Toxic hazardous waste "U" List Ingredient CAS # Status Reference number Formaldehyde 50-00-0 Listed U122

Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Section 14. Transport information Regulatory information UN number Proper shipping name Classes PG* Label Additional information DOT Classification UN2209 FORMALDEHYDE SOLUTION RQ 8 III Reportable quantity (Formaldehyde) 270.27 lbs / 122.7 kg [29. 738 gal / 112.57 L] Package sizes shipped in quantities less than the product reportable quantity are not subject to the RQ (reportable quantity) transportation requirements. TDG Classification UN2209 FORMALDEHYDE SOLUTION 8 III -

Mexico Classification UN2209 FORMALDEHYDE SOLUTION 8 III -

ADR/RID Class UN2209 FORMALDEHYDE SOLUTION 8 III Hazard identification number 80

Article Number Page: 5/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 IMDG Class UN2209 FORMALDEHYDE SOLUTION 8 III -

IATA-DGR Class UN2209 FORMALDEHYDE SOLUTION 8 III -

PG* : Packing group Section 15. Regulatory information HCS Classification Combustible liquid Toxic material Corrosive material Sensitizing material Carcinogen Target organ effects U.S. Federal regulations TSCA 8(a) CDR Exempt/Partial exemption: Not determined United States inventory (TSCA 8b): All components are listed or exempted. Clean Water Act (CWA) 311: Formaldehyde Clean Air Act (CAA) 112 regulated toxic substances: Formaldehyde Clean Air Act Section 112(b) Listed Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Not listed Substances

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

SARA 302 TPQ SARA 304 RQ Name % EHS (lbs) (gallons) (lbs) (gallons) Formaldehyde 37 Yes. 500 73.6 100 14.7

SARA 304 RQ 270.3 lbs / 122.7 kg [29.7 gal / 112.6 L] SARA 311/312 Classification Fire hazard Immediate (acute) health hazard Delayed (chronic) health hazard Composition/information on ingredients Name % Fire Sudden Reactive Immediate Delayed hazard release of (acute) health (chronic) pressure hazard health hazard Formaldehyde 37 Yes. No. No. Yes. Yes.

SARA 313 Product name CAS number Concentration Form R - Reporting Formaldehyde 50-00-0 37 requirements Supplier notification Formaldehyde 50-00-0 37

State regulations Massachusetts The following components are listed: FORMALDEHYDE New York The following components are listed: Formaldehyde New Jersey The following components are listed: FORMALDEHYDE; FORMALIN Pennsylvania The following components are listed: FORMALDEHYDE

Article Number Page: 6/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 Formaldehyde 37%; part of 'Silver Staining Kit, Protein' 17-1150-01 California Prop. 65 WARNING: This product contains a chemical known to the State of California to cause cancer. Ingredient name Cancer Reproductive No significant risk level Maximum acceptable dosage level Formaldehyde Yes. No. 0.04 µg/day (ingestion) No. United States inventory (TSCA 8b) All components are listed or exempted. International regulations International lists Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Japan inventory: All components are listed or exempted. Korea inventory: All components are listed or exempted. Malaysia Inventory (EHS Register): Not determined. New Zealand Inventory of Chemicals (NZIoC): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements COMBUSTIBLE LIQUID AND VAPOR. CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. HARMFUL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED. MAY CAUSE ALLERGIC RESPIRATORY AND SKIN REACTION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. CANCER HAZARD - CONTAINS MATERIAL WHICH CAN CAUSE CANCER. The customer is responsible for determining the PPE code for this material.

2 Flammability National Fire Protection Association (U.S.A.) Health 3 0 Instability Special

Indicates information that has changed from previously issued version. History Date of printing 21 October 2013 Date of previous issue 30 July 2012 Date of issue 21 October 2013 Version 6 Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Article Number Page: 7/7

17115001-3 Validation date 21 October 2013 95171150013 Version 6 GE Healthcare

Material Safety Data Sheet United States English Section 1. Chemical product and company identification Product name Sodium Carbonate; part of 'Silver Staining Kit, Protein' Catalogue Number 17-1150-01 9017115001

Synonym Sodium carbonate (2:1); Carbonic acid sodium salt (1:2); Carbonic acid disodium salt; Carbonic acid, disodium salt; Soda ash; Sodium carbonate, anhydrous Material uses Industrial applications: Analytical chemistry. Research. Other non-specified industry: ORGANIC SYNTHESIS; SMOKELESS POWDER; INDUSTRIAL SOLVENT (NITROCELLULOSE, ALKALOIDS, FATS, WAXES, ETC); ANALYTICAL CHEMISTRY; ANESTHETIC; EXTRACTANT. Product type Solid. Validation date 21 October 2013 Print date 21 October 2013 Supplier GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA England +44 0870 606 1921 In case of emergency US ChemTrec (US) 1-800-424-9300 Canada ChemTrec (US) 1-703-527-3887 2. Hazards identification Physical state Solid. [Crystals. or Powder.] Color White. Grayish-white. Odor Odorless. Signal word WARNING! Hazard statements CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. Precautionary measures Use only with adequate ventilation. Avoid contact with eyes, skin and clothing. Keep container tightly closed. Wash thoroughly after handling. OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Routes of entry Dermal contact. Eye contact. Inhalation. Ingestion. Potential acute health effects Eyes Severely irritating to eyes. Risk of serious damage to eyes. Skin Irritating to skin. Inhalation Irritating to respiratory system. Ingestion No known significant effects or critical hazards. Potential chronic health effects Chronic effects No known significant effects or critical hazards. Carcinogenicity No known significant effects or critical hazards. Mutagenicity No known significant effects or critical hazards. Teratogenicity No known significant effects or critical hazards. Developmental effects No known significant effects or critical hazards. Fertility effects No known significant effects or critical hazards. Inhalation Adverse symptoms may include the following: respiratory tract irritation coughing Ingestion No specific data. Skin Adverse symptoms may include the following: irritation redness

Article Number Page: 1/5

17115001-7 Validation date 21 October 2013 95171150017 Version 6.1 Sodium Carbonate; part of 'Silver Staining Kit, Protein' 17-1150-01 Eyes Adverse symptoms may include the following: pain or irritation watering redness Medical conditions aggravated by None known. over-exposure See toxicological information (Section 11) 3. Composition/information on ingredients Name CAS number % by weight sodium carbonate 497-19-8 100

Section 6. Accidental release measures

Personal precautions No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see Section 8). Environmental precautions Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Methods for cleaning up Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Avoid dust generation. Do not dry sweep. Vacuum dust with equipment fitted with a HEPA filter and place in a closed, labeled waste container. Dispose of via a licensed waste disposal contractor. Note: see Section 1 for emergency contact information and Section 13 for waste disposal. Small spill Move containers from spill area. Avoid dust generation. Using a vacuum with HEPA filter will reduce dust dispersal. Place spilled material in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor. Section 7. Handling and storage

Article Number Page: 2/5

17115001-7 Validation date 21 October 2013 95171150017 Version 6.1 Sodium Carbonate; part of 'Silver Staining Kit, Protein' 17-1150-01 Section 8. Exposure controls/personal protection Consult local authorities for acceptable exposure limits. Recommended monitoring If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological procedures monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference to national guidance documents for methods for the determination of hazardous substances will also be required. Engineering measures Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Hygiene measures Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Personal protection Respiratory A respirator is not needed under normal and intended conditions of product use. Hands Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated. Eyes Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles. Skin Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Environmental exposure controls Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Section 9. Physical and chemical properties

Physical state Solid. [Crystals. or Powder.] Color White. Grayish-white. Odor Odorless. Molecular weight 106

Molecular formula Na2CO3 Boiling/condensation point 1600°C (2912°F) Melting/freezing point 851°C (1563.8°F) Vapor pressure 0 kPa (0 mm Hg) [room temperature] Volatility 0% (w/w) Solubility Soluble in the following materials: cold water and hot water. Section 10. Stability and reactivity

Acute toxicity Product/ingredient name Result Species Dose Exposure sodium carbonate LD50 Oral Rat 4090 mg/kg -

Conclusion/Summary Not available. Chronic toxicity Conclusion/Summary Not available. Irritation/Corrosion Conclusion/Summary Not available. Sensitizer Conclusion/Summary Not available.

Article Number Page: 3/5

17115001-7 Validation date 21 October 2013 95171150017 Version 6.1 Sodium Carbonate; part of 'Silver Staining Kit, Protein' 17-1150-01 Carcinogenicity Conclusion/Summary Not available. Mutagenicity Conclusion/Summary Not available. Teratogenicity Conclusion/Summary Not available. Reproductive toxicity

Conclusion/Summary Not available. Section 12. Ecological information Environmental effects No known significant effects or critical hazards. Aquatic ecotoxicity Product/ingredient name Result Species Exposure sodium carbonate Acute EC50 242000 µg/l Fresh water Algae - Navicula seminulum 96 hours Acute LC50 176000 µg/l Fresh water Crustaceans - Amphipoda 48 hours Acute LC50 265000 µg/l Fresh water Daphnia - Daphnia magna 48 hours Acute LC50 300000 µg/l Fresh water Fish - Lepomis macrochirus 96 hours Conclusion/Summary Not available. Persistence/degradability Conclusion/Summary Not available. Other adverse effects No known significant effects or critical hazards. Section 13. Disposal considerations Waste disposal The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Not classified. Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees.

Section 14. Transport information IATA-DGR Class Classes PG*

Clean Air Act Section 602 Class II Not listed Substances

DEA List I Chemicals (Precursor Not listed Chemicals)

DEA List II Chemicals (Essential Not listed Chemicals)

SARA 302/304 Composition/information on ingredients

No products were found.

Article Number Page: 4/5

17115001-7 Validation date 21 October 2013 95171150017 Version 6.1 Sodium Carbonate; part of 'Silver Staining Kit, Protein' 17-1150-01 SARA 304 RQ Not applicable. SARA 311/312 Classification Immediate (acute) health hazard Composition/information on ingredients Name % Fire Sudden Reactive Immediate Delayed hazard release of (acute) health (chronic) pressure hazard health hazard sodium carbonate 100 No. No. No. Yes. No.

State regulations Massachusetts This material is not listed. New York This material is not listed. New Jersey This material is not listed. Pennsylvania This material is not listed. California Prop. 65 United States inventory (TSCA 8b) This material is listed or exempted. International regulations International lists Australia inventory (AICS): This material is listed or exempted. China inventory (IECSC): This material is listed or exempted. Japan inventory: This material is listed or exempted. Korea inventory: This material is listed or exempted. Malaysia Inventory (EHS Register): This material is listed or exempted. New Zealand Inventory of Chemicals (NZIoC): This material is listed or exempted. Philippines inventory (PICCS): This material is listed or exempted. Taiwan inventory (CSNN): Not determined. Chemical Weapons Convention Not listed List Schedule I Chemicals

Chemical Weapons Convention Not listed List Schedule II Chemicals

Chemical Weapons Convention Not listed List Schedule III Chemicals

Section 16. Other information Label requirements CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. The customer is responsible for determining the PPE code for this material.

0 Flammability National Fire Protection Association (U.S.A.) Health 2 1 Instability Special

Article Number Page: 5/5

17115001-7 Validation date 21 October 2013 95171150017 Version 6.1