PRPF19 Sirna Set I PRPF19 Sirna Set I

Catalog # Aliquot Size

P270-911-05 3 x 5 nmol P270-911-20 3 x 20 nmol P270-911-50 3 x 50 nmol

PRPF19 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P270-911

Lot # Z2085-15

Specificity Formulation PRPF19 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PRPF19 expression. shipped at room temperature.

Product Description Reconstitution Protocol PRPF19 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PRPF19 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_014502. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PRPF19 Protein P270-30G below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background PRPF19 or pre-mRNA-processing factor 19 is the human PRPF19 siRNA Set I homolog of yeast Pso4. PRPF19 is a gene essential for cell siRNA duplexes targeted against three exon regions survival, DNA repair and association with terminal deoxynucleotidyl transferase (1). PRPF19 forms a stable Catalog # P270-911 complex with SETMAR (Metnase) which is a SET and Lot # Z2085-15 transposase fusion protein in humans and plays a positive Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol role in double-strand break (DSB) repair. PRPF19 mediates Format Lyophilized powder association of SETMAR with sites of DNA damage (2). Stability 1yr at -70oC from date of shipment SNEV is an orthologue of PRPF19 and is an evolutionarily Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is conserved splicing factor whose oligomerization is recommended that the lyophilized necessary for spliceosome assembly. and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µ M can References undergo up to 50 freeze-thaw cycles 1. Mahajan, K. N. et.al: Role of human Pso4 in without significant degradation. For mammalian DNA repair and association with terminal long-term storage, it is recommended that the siRNA is stored at -70oC. For deoxynucleotidyl transferase. Proc. Nat. Acad. Sci. most favorable performance, avoid 100: 10746-10751, 2003. repeated handling and multiple 2. Beck, B. D. et.al: Human Pso4 is a Metnase (SETMAR)- freeze/thaw cycles. binding partner that regulates Metnase function in DNA repair. J. Biol. Chem. 283: 9023-9030, 2008.

To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.