Cell Biology
Improve your laboratory workflow What about protection against contamination? Cultivation Under Controlled Conditions
How do I prevent Cell cultivation inhomogeneous cell growth?
Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled Inhomogeneous cell adhesion! conditions, e.g. using single-use vessels. What now?
Cell Biology is a branch of biology that studies cells — the organelles they contain, their function, their physiological properties, their life cycle and the interactions with their environment. Cell Biology basic research can be divided into several subfields; the study of cell metabolism, the study of cellular genetics and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell communication and signaling. Research in cell biology overlaps to a great extent other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology. How to avoid negative effects caused by leachables or extractables? Most frequent problems /challenges Possible solution Eppendorf’s solution Cell culture dishes; CO2 incubators
Contamination of cells Minimize the risk by: Eppendorf’s product benefits:
>>The CO2 incubator can be a source of >>Perform incubator disinfection >>Easy to clean incubators with high temperature Cultivation Under contamination routine regularly disinfection mode >>Medium and cells in contact with >>Fill consumables one by one >>Corrugated handling ring of Cell Culture Dishes outside air due to splashing and >>Use flasks with a screw cap instead of dishes for safe transportation inadvertent removal of the lid with a loose lid e. g. for maintenance of stock >>SplashProtect™ ring in dishes to avoid splashes Controlled Conditions cultures or when frequent transportation of and bridging of medium with outside environment the cultures is needed >>Flasks with optimized geometry
Most frequent problems /challenges Possible solution Eppendorf’s solution Cell culture plates; CO2 incubators
Inhomogeneous cell growth Minimize the risk by: Eppendorf’s product benefits: Cell cultivation >>Inhomogeneous distribution of cells in the >>Omit usage of the edge wells >>Peripheral moat in 96-well Cell Culture Plates edge wells of multiwell plates >>Increase sedimentation time for cells to minimize edge effect >>Higher evaporation rate in edge wells in the 96-well plate at room temperature >>Possibility to fill the inter well space of all
>>Temperature variations during incubation >>Use a separate CO2 incubator for cells Cell Culture Plate formats to reduce temperature variations ® >>Variations in chamber atmosphere due to door seeded for assays >>Usage of split inner doors of Galaxy CO2 incubator
opening of the CO2 incubator >>Minimize door opening events for stable growth conditions
of the CO2 incubator
Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in Most frequent problems /challenges Possible solution Eppendorf’s solution Pipettes and tips; Dishes, flasks and plates handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled Inhomogeneous cell adhesion Minimize the risk by: Eppendorf’s product benefits: conditions, e.g. using single-use vessels. >>Variations in seeding cell numbers >>Regular resuspension of >>Fast seeding operation using >>Formation of air bubbles during the cells during seeding Multipette® (U.S. / CAN: Repeater®) and Combitips® seeding process >>Manual movement of consumable >>Dispension steps for avoiding air bubble insertion Cell Biology is a branch of biology that studies cells — the organelles they contain, >>Uneven distribution of cells after seeding >>Batch tested and certified consumables their function, their physiological properties, their life cycle and the interactions >>Cell culture consumables with for consistent quality with their environment. Cell Biology basic research can be divided into several high quality standard subfields; the study of cell metabolism, the study of cellular genetics and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell communication and signaling. Research in cell biology overlaps to a great extent other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology. Most frequent problems /challenges Possible solution Eppendorf’s solution Bioprocess systems; Single-use vessels
Issues related to leachables & extractables Minimize the risk by: Eppendorf’s product benefits: >>Cytotoxic effects >>Extractables studies to identify >>Single-use vessels produced >>Altered product characteristics and quantify potentially harmful from monolayer injection-molded compounds plastics: materials comply with >>Rigid-wall stirred tank bioreactors USP Class VI >>Virgin raw materials, all sourced by Eppendorf directly Being in Process
Cell Biology (Eukaryotic cells) Micromanipulators Microinjectors Capillaries Centrifuges Photometers
Cell manipulation
Pipette controllers Serological pipettes Automation Dishes, flasks and plates Conical tubes Cell seeding
Cell cultivation Bioreactors Bioprocess systems Dishes, flasks and plates CO2 incubators Pipettes and tips
Harvesting and preparation
Centrifuges Biopur® consumables Tubes and plates Thermo mixing devices Conical tubes
Analysis and detection
Photometers Cuvettes Thermocyclers PCR consumables Cell imaging consumables Storage
Freezers Deepwell plates Sealing options Concentrator Conical tubes
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