Biochemistry and Cell Biology (BCCB)
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Glossary - Cellbiology
1 Glossary - Cellbiology Blotting: (Blot Analysis) Widely used biochemical technique for detecting the presence of specific macromolecules (proteins, mRNAs, or DNA sequences) in a mixture. A sample first is separated on an agarose or polyacrylamide gel usually under denaturing conditions; the separated components are transferred (blotting) to a nitrocellulose sheet, which is exposed to a radiolabeled molecule that specifically binds to the macromolecule of interest, and then subjected to autoradiography. Northern B.: mRNAs are detected with a complementary DNA; Southern B.: DNA restriction fragments are detected with complementary nucleotide sequences; Western B.: Proteins are detected by specific antibodies. Cell: The fundamental unit of living organisms. Cells are bounded by a lipid-containing plasma membrane, containing the central nucleus, and the cytoplasm. Cells are generally capable of independent reproduction. More complex cells like Eukaryotes have various compartments (organelles) where special tasks essential for the survival of the cell take place. Cytoplasm: Viscous contents of a cell that are contained within the plasma membrane but, in eukaryotic cells, outside the nucleus. The part of the cytoplasm not contained in any organelle is called the Cytosol. Cytoskeleton: (Gk. ) Three dimensional network of fibrous elements, allowing precisely regulated movements of cell parts, transport organelles, and help to maintain a cell’s shape. • Actin filament: (Microfilaments) Ubiquitous eukaryotic cytoskeletal proteins (one end is attached to the cell-cortex) of two “twisted“ actin monomers; are important in the structural support and movement of cells. Each actin filament (F-actin) consists of two strands of globular subunits (G-Actin) wrapped around each other to form a polarized unit (high ionic cytoplasm lead to the formation of AF, whereas low ion-concentration disassembles AF). -
Bacterial Cell Membrane
BACTERIAL CELL MEMBRANE Dr. Rakesh Sharda Department of Veterinary Microbiology NDVSU College of Veterinary Sc. & A.H., MHOW CYTOPLASMIC MEMBRANE ➢The cytoplasmic membrane, also called a cell membrane or plasma membrane, is about 7 nanometers (nm; 1/1,000,000,000 m) thick. ➢It lies internal to the cell wall and encloses the cytoplasm of the bacterium. ➢It is the most dynamic structure of a prokaryotic cell. Structure of cell membrane ➢The structure of bacterial plasma membrane is that of unit membrane, i.e., a fluid phospholipid bilayer, composed of phospholipids (40%) and peripheral and integral proteins (60%) molecules. ➢The phospholipids of bacterial cell membranes do not contain sterols as in eukaryotes, but instead consist of saturated or monounsaturated fatty acids (rarely, polyunsaturated fatty acids). ➢Many bacteria contain sterol-like molecules called hopanoids. ➢The hopanoids most likely stabilize the bacterial cytoplasmic membrane. ➢The phospholipids are amphoteric molecules with a polar hydrophilic glycerol "head" attached via an ester bond to two non-polar hydrophobic fatty acid tails. ➢The phospholipid bilayer is arranged such that the polar ends of the molecules form the outermost and innermost surface of the membrane while the non-polar ends form the center of the membrane Fluid mosaic model ➢The plasma membrane contains proteins, sugars, and other lipids in addition to the phospholipids. ➢The model that describes the arrangement of these substances in lipid bilayer is called the fluid mosaic model ➢Dispersed within the bilayer are various structural and enzymatic proteins, which carry out most membrane functions. ➢Some membrane proteins are located and function on one side or another of the membrane (peripheral proteins). -
Intelligent Design, Abiogenesis, and Learning from History: Dennis R
Author Exchange Intelligent Design, Abiogenesis, and Learning from History: Dennis R. Venema A Reply to Meyer Dennis R. Venema Weizsäcker’s book The World View of Physics is still keeping me very busy. It has again brought home to me quite clearly how wrong it is to use God as a stop-gap for the incompleteness of our knowledge. If in fact the frontiers of knowledge are being pushed back (and that is bound to be the case), then God is being pushed back with them, and is therefore continually in retreat. We are to find God in what we know, not in what we don’t know; God wants us to realize his presence, not in unsolved problems but in those that are solved. Dietrich Bonhoeffer1 am thankful for this opportunity to nature, is the result of intelligence. More- reply to Stephen Meyer’s criticisms over, this assertion is proffered as the I 2 of my review of his book Signature logical basis for inferring design for the in the Cell (hereafter Signature). Meyer’s origin of biological information: if infor- critiques of my review fall into two gen- mation only ever arises from intelli- eral categories. First, he claims I mistook gence, then the mere presence of Signature for an argument against bio- information demonstrates design. A few logical evolution, rendering several of examples from Signature make the point my arguments superfluous. Secondly, easily: Meyer asserts that I have failed to refute … historical scientists can show that his thesis by not providing a “causally a presently acting cause must have adequate alternative explanation” for the been present in the past because the origin of life in that the few relevant cri- proposed candidate is the only known tiques I do provide are “deeply flawed.” cause of the effect in question. -
Standard 2: CELL BIOLOGY – REVIEW of BASICS
Standard 2: CELL BIOLOGY – REVIEW OF BASICS CELL PART OR TYPE OF CELL WHERE FOUND WHAT DOES IT FUNCTION: MISCELLANEOUS ORGANELLE Prokaryotic cell Plant cell LOOK LIKE: Job it does in INFORMATION: things Eukaryotic cell Animal cell Describe or Draw the cell such as color, what it is Both Both made of, size, etc. plasma/cell See diagram Holds cell together Phospholipid bilayer with membrane both both Regulates what goes proteins in/out of cell Semipermeable cytoplasm both Clear thick jelly- Supports/protects both like material in cell cell organelles See diagram Control center nucleus eukaryotic both Contains DNA See diagram Where proteins are ribosome both both made See diagram Process proteins Golgi complex eukaryotic both that go to other /apparatus parts of cell Membrane-bound Digests materials lysosome eukaryotic animal sac of digestive within the cell enzymes Membrane-bound Stores water, food, One large one in plants vacuole eukaryotic both storage area waste and dissolved Many smaller ones in minerals animals endoplasmic Network of Transport materials Can be rough (with reticulum eukaryotic both membrane tubes throughout the cell ribosomes attached) or smooth (without ribosomes) See diagram Where cell respiration Called Powerhouse of cell mitochondria eukaryotic both occurs (releases Makes ATP from energy for cell to use) breaking down glucose See diagram Where photosynthesis Contains chlorophyll chloroplast eukaryotic plant takes place Converts light energy into chemical energy in glucose Some pro- and plant (also fungi Rigid structure -
Introduction to the Cell Cell History Cell Structures and Functions
Introduction to the cell cell history cell structures and functions CK-12 Foundation December 16, 2009 CK-12 Foundation is a non-profit organization with a mission to reduce the cost of textbook materials for the K-12 market both in the U.S. and worldwide. Using an open-content, web-based collaborative model termed the “FlexBook,” CK-12 intends to pioneer the generation and distribution of high quality educational content that will serve both as core text as well as provide an adaptive environment for learning. Copyright ©2009 CK-12 Foundation This work is licensed under the Creative Commons Attribution-Share Alike 3.0 United States License. To view a copy of this license, visit http://creativecommons.org/licenses/by-sa/3.0/us/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA. Contents 1 Cell structure and function dec 16 5 1.1 Lesson 3.1: Introduction to Cells .................................. 5 3 www.ck12.org www.ck12.org 4 Chapter 1 Cell structure and function dec 16 1.1 Lesson 3.1: Introduction to Cells Lesson Objectives • Identify the scientists that first observed cells. • Outline the importance of microscopes in the discovery of cells. • Summarize what the cell theory proposes. • Identify the limitations on cell size. • Identify the four parts common to all cells. • Compare prokaryotic and eukaryotic cells. Introduction Knowing the make up of cells and how cells work is necessary to all of the biological sciences. Learning about the similarities and differences between cell types is particularly important to the fields of cell biology and molecular biology. -
Biochemistry Biotechnology Cell Biology
Undergraduate Biochemistry Opportunities www.ed.ac.uk/biology Biotechnology Cell Biology Biochem_Biotech_CellBio_A5.indd 1 21/05/2019 14:25 Biochemistry The programme combines coverage of the Biochemistry is the study of living systems at basic principles and knowledge underpinning the cellular and molecular level. This dynamic biotechnology and an appreciation of the field draws on a variety of subjects and has processes involved in converting an idea widespread application. Biochemistry applies a into a product. The objective is to provide a knowledge of chemistry and physical sciences firm foundation in molecular and microbial to investigate basic life processes. The subject biotechnology through compulsory sections has a major impact on modern medical research dealing with topics such as expression vectors, and upon the pharmaceutical, bioengineering, microbial fermentation, protein structure, drug agricultural and environmental industries. design and the development of antimicrobials and vaccines. The programme encourages the critical assessment of current developments in areas of Cell Biology biological interest. Modern cell biology is a dynamic discipline that combines the interests and techniques of many Biotechnology scientific fields. Cell biologists investigate the Biotechnology is concerned with industrial basic structural and functional units of life, the and biomedical applications of fundamental cells that compose all living organisms. They aim knowledge derived from biology. This covers to understand: cellular structure, composition many facets from making useful products and regulation, the organelles that cells contain, using microbial, plant or animal cells to using cell growth, nuclear and cellular division, and bioinformatics and structural biology to design cell death. Understanding how cells work is new drugs. Biotechnology is an exciting area fundamental to many areas of biology and is of with new developments each year in areas that particular importance to fields such as cancer affect us all. -
Physiology, Biochemistry, and Pharmacology of Transporters for Organic Cations
International Journal of Molecular Sciences Editorial Physiology, Biochemistry, and Pharmacology of Transporters for Organic Cations Giuliano Ciarimboli Experimental Nephrology, Department of Internal Medicine D, University Hospital Münster, 48149 Münster, Germany; [email protected] This editorial summarizes the 13 scientific papers published in the Special Issue “Physiology, Biochemistry, and Pharmacology of Transporters for Organic Cations” of the International Journal of Molecular Sciences. In this Special Issue, the readers will find integrative information on transporters for organic cations. Besides reviews on physi- ology, pharmacology, and toxicology of these transporters [1–3], which offer a concise overview of the field, the readers will find original research work focusing on specific transporter aspects. Specifically, the review “Organic Cation Transporters in Human Physiology, Phar- macology, and Toxicology” by Samodelov et al. [1] summarizes well the general as- pects of physiology, pharmacology, and toxicology of transporter for organic cations. The other review “Organic Cation Transporters in the Lung—Current and Emerging (Patho)Physiological and Pharmacological Concepts” by Ali Selo et al. [2] focuses on these aspects of transporters for organic cations in the lung, an important but often ne- glected field. In the paper “Systems Biology Analysis Reveals Eight SLC22 Transporter Subgroups, Including OATs, OCTs, and OCTNs”, by performing a system biology analysis of SLC22 transporters, Engelhart et al. [4] suggest the existence of a transporter–metabolite network. They propose that, in this network, mono-, oligo-, and multi-specific SLC22 transporters Citation: Ciarimboli, G. Physiology, interact to regulate concentrations and fluxes of many metabolites and signaling molecules. Biochemistry, and Pharmacology of In particular, the organic cation transporters (OCT) subgroup seems to be associated with Transporters for Organic Cations. -
BIOCHEMISTRY MAJOR the Biochemistry Major Is Offered Through the Department of Chemistry
BIOCHEMISTRY MAJOR The Biochemistry major is offered through the Department of Chemistry. This sequence is recommended for students planning entry into graduate school in Biochemistry, Pharmacology, or related subjects. It is also strongly recommend for those desiring to pursue medical or dental school. NOTE: This course sequence may be modified to include a Senior Capstone Experience. FIRST YEAR Fall Semester Credits Spring Semester Credits General Chemistry I for Majors (CHE111) 3 General Chemistry II for Majors (CHE112) 3 General Chemistry I Lab for Majors (CHE111L) 1 General Chemistry II Lab for Majors (CHE112L) 1 General Chemistry I Récitation (CHE111R) 0 General Chemistry II Récitation (CHE112R) 0 Precalculus (MAT116 or MAT120) 3-4 Calculus I (MAT231) 4 First Year Composition (ENG103) 4 Foreign Language (FL201) 4 African Diaspora/World I (ADW111) 4 African Diaspora/World II (ADW112) 4 First Year Experience (FYE 101-Chemistry) 1 First Year Experience (FYE 102-Chemistry) 1 First Year Seminar in Chemistry (CHE101) 0 Big Questions Colloquia (BQC) 1 TOTAL HOURS 16-17 TOTAL HOURS 18 SOPHOMORE YEAR Fall Semester Credits Spring Semester Credits Organic Chemistry I for Majors (CHE231) 4 Organic Chemistry II for Majors (CHE232) 4 Organic Chemistry I Lab (CHE233L) 1 Organic Chemistry II Lab (CHE234L) 1 Organic Chemistry I Récitation (CHE233R) 0 Organic Chemistry II Récitation (CHE234R) 0 Biology of the Cell (BIO120) 4 Organismal Form and Function(BIO115) 4 Calculus II (MAT 232) 4 Physics I: Mechanics & Lab (PHY151) 4 Foreign Language (FL202) -
Independent Research Resources Demonstrations/Simulations
Independent Research Resources Independent Generation of Research (IGoR) - IGoR provides a platform for people to pool their knowledge, resources, time, and creativity so that everyone can pursue their own scientific curiosity. Virginia Junior Academy of Science Resource Library - Extensive collection of open-access resources for students in Biology & Medicine, Botany, Ecology, Environmental Sciences, Chemistry, Engineering and Physics The Society for Science and the Public Science Project Resources - A catalog of science resources that can support your quest to learn and do science Science Buddies - Ideas for science projects Teacher resources National Center for Science Education Scientist in the Classroom - Platform allows teachers to request classroom visits from scientists Genetics Education Outreach Network (GEON) - Network of genetics professionals HHMI BioInteractive Data Points - Explore and interpret primary data from published research Biotech in a Box Loan Kits - Shipped to your school from Fralin Life Sciences Institute at Virginia Tech Demonstrations/Simulations Genetic Science Learning Center- Simulations, videos and interactive activities that explore genetics, cell biology, neuroscience, ecology and health Remotely Accessible Instruments for Nanotechnology (RAIN) - Access and control nanoinstruments over the Internet in real-time with the assistance of an experienced engineer PhET Simulations - Interactive STEM simulations for all grade levels HHMI BioInteractive Interactive Media - Recommendations: Virus Explorer; Exploring -
Biochemistry and Molecular Biology
School of Life Sciences / Department of Biochemistry and Biophysics BIOCHEMISTRY AND MOLECULAR BIOLOGY Revealing how life works Department of Biochemistry and Biophysics Oregon State University 2011 Agriculture and Life Sciences Building Corvallis, OR 97331 OSUBB 541-737-4511 biochem.oregonstate.edu College of Science Oregon State University 128 Kidder Hall Corvallis, OR 97331 541-737-4811 science.oregonstate.edu This publication will be made available in an accessible alternative format upon request. Please contact the College COLLEGE OF SCIENCE of Science at 541-737-4811 or [email protected]. ACADEMIC BROCHURE / 2020 Highlights • Solve problems at the What will you discover? intersection of biological and physical sciences with our The Department of Biochemistry and Biophysics offers nationally accredited program world-class faculty, a tradition of interdisciplinary in biochemistry, molecular and research, teaching excellence and extraordinary cellular biology, chemistry, laboratories to facilitate undergraduate learning. molecular genetics, physics and statistics. The department ranks high nationally and internationally in many research areas, including signal transduction, gene • Tailor your education to expression, epigenetics, metabolic regulation, structural specific career goals with three biology, and genetic code expansion technology. different options. The department offers two Bachelor of Science degrees, • Thrive in a smaller, supportive both accredited by the American Society for Biochemistry department within a world-class and Molecular Biology (ASBMB): research university. • Biochemistry and Molecular Biology (BMB) with • Pursue interdisciplinary research options in Advanced Molecular Biology, Computational projects with faculty across OSU. Molecular Biology, and Pre-medicine; • Participate in the Biochemistry • Biochemistry and Biophysics (BB) with options in Club for community, leadership Advanced Biophysics, Neuroscience, and Pre-medicine. -
Cell Structure and Function in the Bacteria and Archaea
4 Chapter Preview and Key Concepts 4.1 1.1 DiversityThe Beginnings among theof Microbiology Bacteria and Archaea 1.1. •The BacteriaThe are discovery classified of microorganismsinto several Cell Structure wasmajor dependent phyla. on observations made with 2. theThe microscope Archaea are currently classified into two 2. •major phyla.The emergence of experimental 4.2 Cellscience Shapes provided and Arrangements a means to test long held and Function beliefs and resolve controversies 3. Many bacterial cells have a rod, spherical, or 3. MicroInquiryspiral shape and1: Experimentation are organized into and a specific Scientificellular c arrangement. Inquiry in the Bacteria 4.31.2 AnMicroorganisms Overview to Bacterialand Disease and Transmission Archaeal 4.Cell • StructureEarly epidemiology studies suggested how diseases could be spread and 4. Bacterial and archaeal cells are organized at be controlled the cellular and molecular levels. 5. • Resistance to a disease can come and Archaea 4.4 External Cell Structures from exposure to and recovery from a mild 5.form Pili allowof (or cells a very to attach similar) to surfacesdisease or other cells. 1.3 The Classical Golden Age of Microbiology 6. Flagella provide motility. Our planet has always been in the “Age of Bacteria,” ever since the first 6. (1854-1914) 7. A glycocalyx protects against desiccation, fossils—bacteria of course—were entombed in rocks more than 3 billion 7. • The germ theory was based on the attaches cells to surfaces, and helps observations that different microorganisms years ago. On any possible, reasonable criterion, bacteria are—and always pathogens evade the immune system. have been—the dominant forms of life on Earth. -
And Even-Length, Medium-Chain Fatty Acids in Plants (Amino Adds/Elongtin) ANTOANETA B
Proc. Nadl. Acad. Sci. USA Vol. 91, pp. 11437-11441, November 1994 Biochemistry A pathway for the biosynthesis of straight and branched, odd- and even-length, medium-chain fatty acids in plants (amino adds/elongtin) ANTOANETA B. KROUMOVA, ZHIYI XIE, AND GEORGE J. WAGNER* Plant Physiology/Biochemistry/Molecular Biology Program, Agronomy Department, University of Kentucky, Lexington, KY 40546-0091 Communicated by Martin Gibbs, July 25, 1994 ABSTRACT Pathways and enzymes offatty acid synthase- and even-length scFAs are synthesized via modified mediated, long-even-chain (generally C16-C20) fatty add syn- branched-chain amino acid (bcAA) metabolism in tobacco thesis are well studied, and general metabolism involved in trichome glands (6, 7). It is possible that at least branched and short-chain (Cs-C7) fatty acid biosynthesis is also understood. odd-length mcFAs are formed in this tissue similarly. Alter- In contrast, mechanism of medinm-chain (C#-C14) fatty acid natively, primers derived from bcAA metabolism may be synthesis are unclear. Recent work suggests involvement of elongated by fatty acid synthase (FAS), as suggested for chain-elongation-terminating thloesterases in medium-chain tomato trichomes (8) and tobacco epidermis (9). fatty acid formation in oilseeds and animals. We have shown The classical pathway for bcAA (Val, Leu, Ileu) biosyn- that iso- and anteiso-branched and straigbt, odd- and even- thesis in microorganisms (and largely by inference in plants, length, short-chain fatty adds esterifled in plant-trichome- ref. 10) is shown in the shaded areas of Fig. 1. Key activities gland-produced sucrose esters are synthesized by using carbon involved in branched-chain formation (reactions 1, 1A, and 2) skeletons provided by modified branched-chain amino acid are those catalyzing leucine biosynthesis in all organisms and metabolism/catablis.