Wine profiling by1 H-NMR Nuclear Magnetic Resonance (Wine Screener 3.1 release)

Wine authenticity Grape variety control Developed in partnership with the Available models for • Pinot Noir instrument manufacturer Bruker Biospin discrimination of : • Tempranillo and with a network of partners supplying • Garnacha Tinta authentic samples, the Proton Nuclear • Syrah Magnetic Resonance Profiling 1 ( H-NMR) • Merlot Noir Wine Screener provides a comprehensive • Cabernet Sauvignon authenticity and quality control screening • Sangiovese test in only one processing step. • Nebbiolo • Montepulciano Analysis principle Red wine • Primitivo • Dornfelder 1H-NMR analysis generates a spectrum representing the unique fingerprint of the • Portugieser Blau tested wine. The obtained profile allows to • Zweigeltrebe Blau obtain a large range of information, which • Chardonnay Blanc are both: • Sauvignon Blanc • Riesling • Targeted, for quantification of interest • Pinot Blanc/Gris compounds • Silvaner • Untargeted, that might indicate • Verdejo contaminations or reveal anomalies in • Mueller Thurgau the assayed sample • Veltliner • Moscatel Advantages of the method • Welschriesling This screening method : • Covers a large scope of potential Geographical origins control issues Countries Regions Appellation • Detects counterfeiting, mislabelling or France Bordeaux, Burgundy, Chablis Languedoc, Rhône Val- quality deviation ley, Loire Valley Italy Piemonte, Toscana, Valpolicella • Provides a quick response even when Sicilia, Puglia a large number of samples has to be Spain La Rioja, analyzed Ribera del Duero Germany Austria Chile Quantified parameters • Total Alcohol Vol% • Benzoic acid • Ethanol Stabilizing • Sorbic acid

• Glycerol Agents • Salicylic acid

• Glucose • Caftaric acid • Fructose • Epicatechin • Sucrose (Poly-)phenols • Gallic acid

Standard • Arabinose • Shikimic acid Parameters • Total sugar • Trigonelline • Tartric acid • 4-aminobutanoic acid • Malic acid • Alanine • Lactic acid Amino Acids • Arginine • Citric acid • Proline • Energy value • Acetic acid • Acetoine Verification of vintage • Ethylacetate Available for white wines from the three • Ethyllactate previous years at the time of measurement • Formic acid Degradation • Fumaric acid Parameters Analytical reports include • Gluconic acid • Putrescine • Quantification of more than 50 • Cadaverine compositional characteristic parameters • HMF of wines and their comparison with standard values • Furfural • Methanol • Confirmation of grape variety for single

• 1,3-propanediol variety wine listed

• 2,3-butanediol • Confirmation of geographical origin for Higher Alcohols/ • 2-methyl-propanol listed countries and region Fermentation Products • 3-methyl-butanol • Detection of dilution with water • Acetaldehyde • Verification of vintage • Pyvuric acid • An interpretation by our experts • Galacturonic acid including, in case of discrepancy, • Succinic acid recommendations for follow-up actions after this screening step © Eurofins Food France - 0916

Eurofins Analytics France www.eurofins.fr Tel: +33 (0)2 51 83 43 40 Authenticity Competence Center [email protected] Fax: +33 (0)2 51 83 21 11 9, rue Pierre Adolphe Bobierre 44300 Nantes - France