Information Sheet for NR-19544

Salmonella enterica subsp. enterica, Strain supplemented with 15% glycerol. ® Ty2 (Serovar Typhi), Gateway Clone Set, Packaging/Storage: Recombinant in Escherichia coli, Plate 23 NR-19544 was packaged aseptically in a 96-well plate. The product is provided frozen and should be stored at -80°C or Catalog No. NR-19544 colder immediately upon arrival. For long-term storage, the This reagent is the tangible property of the U.S. Government. vapor phase of a liquid freezer is recommended. Freeze-thaw cycles should be avoided.

For research use only. Not for human use. Growth Conditions:

Media: Contributor: LB broth or agar containing 50 µg/mL kanamycin. Pathogen Functional Genomics Resource Center at the J. Incubation: Craig Venter Institute Temperature: 37°C Atmosphere: Aerobic Manufacturer: Propagation: BEI Resources 1. Scrape top of frozen well with a pipette tip and streak onto agar plate. Product Description: 2. Incubate the plates at 37°C for 24 hours. Clone plates are replicated using a BioMek® FX robot. Production in the 96-well format has increased risk of cross- Citation: contamination between adjacent wells. Individual clones Acknowledgment for publications should read “The following should be purified (e.g. single colony isolation and reagent was obtained through BEI Resources, NIAID, purification using good microbiological practices) and NIH: Salmonella enterica subsp. enterica, Strain Ty2 sequence-verified prior to use. BEI Resources only confirms (Serovar Typhi), Gateway® Clone Set, Recombinant in the clone plate orientation and viability of randomly picked Escherichia coli, Plate 23, NR-19544.” clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor. Biosafety Level: 1 Appropriate safety procedures should always be used with The Salmonella enterica subsp. enterica (S. enterica subsp. this material. Laboratory safety is discussed in the following ® enterica), strain Ty2 (serovar Typhi), Gateway clone set publication: U.S. Department of Health and Human Services, consists of approximately 3380 sequence validated clones Public Health Service, Centers for Disease Control and from S. enterica subsp. enterica, strain Ty2, cloned in Prevention, and National Institutes of Health. Biosafety in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading Microbiological and Biomedical Laboratories. 5th ed. frame was constructed in vector pDONR221 (Invitrogen) Washington, DC: U.S. Government Printing Office, 2009; see with an ATG start codon and no stop codon. The sequence www.cdc.gov/biosafety/publications/bmbl5/index.htm. was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than Disclaimers: 0.2%. Detailed information about each clone is shown in You are authorized to use this product for research use only. Table 1. It is not intended for human use.

® Information related to the use of Gateway Clones can be Use of this product is subject to the terms and conditions of obtained from Invitrogen. Recombination was facilitated the BEI Resources Material Transfer Agreement (MTA). The through an attB (attB-PCR product or a linearized MTA is available on our Web site at www.beiresources.org. attB expression clone) with an attP substrate (pDONR221) to create an attL-containing entry clone. The entry clone While BEI Resources uses reasonable efforts to include contains recombinational cloning sites, attL1 and attL2 to accurate and up-to-date information on this product sheet, facilitate gene transfer into a destination vector, M13 forward neither ATCC® nor the U.S. Government makes any and reverse priming sites for sequencing and a kanamycin warranties or representations as to its accuracy. Citations resistance gene for selection. Please refer to the Invitrogen from scientific literature and patents are provided for Gateway® Technology Manual for additional details. informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been Plate orientation and viability were confirmed for NR-19544. confirmed to be accurate.

Material Provided: This product is sent with the condition that you are Each inoculated well of the 96-well plate contains responsible for its safe storage, handling, use and disposal. approximately 60 µL of E. coli culture (strain DH10B-T1) in ATCC® and the U.S. Government are not liable for any Luria Bertani (LB) broth containing 50 µg/mL kanamycin damages or injuries arising from receipt and/or use of this

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Product Information Sheet for NR-19544

product. While reasonable effort is made to ensure its derivatives must contact the contributor to determine if a authenticity and reliability of materials on deposit, the U.S. license is required. U.S. Government contractors may need Government, ATCC®, their suppliers and contributors to BEI a license before first commercial sale. Resources are not liable for damages arising from the misidentification or misrepresentation of products. References: 1. Deng, W., et al. “Comparative Genomics of Salmonella Use Restrictions: enterica serovar Typhi strains Ty2 and CT18.” J. This material is distributed for internal research, non- Bacteriol. 185 (2003): 2330-2337. PubMed: 12644504. commercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as ATCC® is a trademark of the American Type Culture performed under a U.S. Government contract, individuals Collection. contemplating commercial use of the material, its products or

Table 1: Salmonella enterica subsp. enterica, Strain Ty2 (Serovar Typhi), Gateway® Clone Set, Recombinant in Escherichia coli, Plate 23 (ZSTDW)1 Well ORF Accession Average Depth of Clone Locus ID Description Position Length Number Coverage 92265 A01 t1643 hypothetical protein t1643 913 NP_805421.1 1.423877 92070 A02 t2206 hemolysin-like protein 913 NP_805950.1 2 92057 A03 t2341 2-hydroxy-3-oxoproprionate reductase 913 NP_806074.1 1.757941 inorganic polyphosphate/ATP-NAD 92146 A04 t2637 913 NP_806348.1 2 92274 A05 t3195 hypothetical protein t3195 913 NP_806873.1 1.702081 92289 A06 t3599 aldolase 913 NP_807242.1 2.950712 92031 A07 t1034 heat shock protein HtpX 916 NP_804852.1 2.136463 92114 A08 t1794 transcriptional regulator 916 NP_805567.1 1.959607 92199 A09 t1926 DNA methylase 916 NP_805693.1 1.449782 92017 A10 t3298 ribosomal protein L11 methyltransferase 916 NP_806973.1 1.858079 92213 A11 t3838 sugar kinase 916 NP_807459.1 1.99345 92373 A12 t0672 cytidine deaminase 919 NP_804521.1 3.972797 92033 B01 t0739 hypothetical protein t0739 919 NP_804583.1 1.570185 92354 B02 t3077 919 NP_806762.1 1.553863 92337 B03 t3199 lipoprotein Nlpl 919 NP_806877.1 1.843308 92322 B04 t3301 methyltransferase 919 NP_806976.1 1.853101 92298 B05 t3342 chloramphenicol-sensitive protein RarD 919 NP_807010.1 1.927095 92257 B06 t0954 flagellar motor protein MotA 922 NP_804778.1 1.687636 92082 B07 t2496 2-methylisocitrate 922 NP_806224.1 1.801518 92157 B08 t2769 AraC family transcriptional regulator 922 NP_806470.1 2.752711 glycerol-3-phosphate transporter 92066 B09 t3965 922 NP_807575.1 1.996746 permease 92042 B10 t4176 LysR family transcriptional regulator 922 NP_807780.1 1.984816 iron-hydroxamate transporter substrate- 92349 B11 t0201 925 NP_804083.1 3.092973 binding protein peptide transport system permease 92230 B12 t1608 925 NP_805386.1 1.837838 SapC 92206 C01 t2421 protoheme IXfarnesyltransferase 925 NP_806151.1 1.548108 92105 C02 t3668 phosphate transporter permease PtsA 925 NP_807304.1 2.151351 92250 C03 t4133 maltose ABC transporter permease 925 NP_807737.1 1.502703 92162 C04 t4342 methyltransferase 925 NP_807935.1 1.972973 92293 C05 t0151 quinolinate phosphoribosyltransferase 928 NP_804034.1 1.856681 N-acetylmuramic acid-6-phosphate 92262 C06 t0286 928 NP_804162.1 1.975216 etherase 92334 C07 t2156 glycosyl 928 NP_805907.1 1.794181

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Product Information Sheet for NR-19544

Well Locus ORF Accession Average Depth Clone Description Position ID Length Number of Coverage 92102 C08 t2329 carbamate kinase 928 NP_806063.1 1.883621 92381 C09 t3256 N-acetylneuraminate lyase 928 NP_806931.1 3.984914 92246 C10 t0193 glutamyl-Q tRNA(Asp) synthetase 931 NP_804076.1 1.588614 92045 C11 t1307 oxidoreductase 931 NP_805108.1 2.140709 4-hydroxyphenylacetate 3- 92190 C12 t1811 monooxygenase operon regulatory 931 NP_805584.1 1.37594 protein 92270 D01 t2676 transcriptional regulator 931 NP_806387.1 1.870032 92562 D02 t3155 LysR family transcriptional regulator 931 NP_806836.1 1.997852 92401 D03 t3558 ferrous iron efflux protein F 931 NP_807203.1 1.978518 92513 D04 t3598 oxidoreductase 931 NP_807241.1 2.452202 92741 D05 t0042 transcriptional activator NhaR 934 NP_803926.1 2.819058 92397 D06 t0407 coproporphyrinogen III oxidase 934 NP_804276.1 1.911135 92446 D07 t0563 hypothetical protein t0563 934 NP_804420.1 1.671306 92674 D08 t0776 dTDP-4-dehydrorhamnose reductase 934 NP_804618.1 1.85546 92691 D09 t0802 ATP phosphoribsyltransferase 934 NP_804644.1 1.87045 92449 D10 t1408 regulatory protein 934 NP_805203.1 2.528908 92649 D11 t1995 hypothetical protein t1995 934 NP_805758.1 1.594218 pyruvate formate-lyase 3 activating 92749 D12 t2045 934 NP_805808.1 3.208779 protein 92486 E01 t2440 geranyltranstransferase 934 NP_806170.1 1.283726 92586 E02 t3334 hypothetical protein t3334 934 NP_807002.1 1.701285 92713 E03 t4266 hypothetical protein t4266 934 NP_807864.1 1.917559 92422 E04 t4288 hypothetical protein t4288 934 NP_807886.1 2 92642 E05 t0816 hypothetical protein t0816 937 NP_804656.1 1.965848 ABC transporter periplasmic binding 92521 E06 t1414 937 NP_805209.1 1.287086 protein 92655 E07 t1438 O-acetylserine/cysteine export protein 937 NP_805231.1 2.152615 92506 E08 t2021 ribosomal protein S6 modification protein 937 NP_805784.1 1.545358 92453 E09 t2262 LysR family transcriptional regulator 937 NP_806004.1 1.596585 92705 E10 t2593 hypothetical protein t2593 937 NP_806310.1 1.824973 92685 E11 t3753 hypothetical protein t3753 937 NP_807383.1 1.640342 92501 E12 t3782 regulatory protein 937 NP_807404.1 1.591249 92517 F01 t3910 LysR family transcriptional regulator 937 NP_807524.1 1.577375 92494 F02 t0276 ribonuclease III 940 NP_804152.1 2 92621 F03 t1665 hypothetical protein t1665 940 NP_805442.1 1.807447 92469 F04 t4217 hypothetical protein t4217 940 NP_807819.1 1.409574 92473 F05 t0065 CitG protein 943 NP_803949.1 1.878049 92537 F06 t0675 transcriptional regulator 943 NP_804524.1 1.490986 92405 F07 t1188 nucleotide excision repair endonuclease 943 NP_804995.1 1.434783 UTP-glucose-1-phosphate 92737 F08 t1663 943 NP_805440.1 1.955461 uridylyltransferase 92549 F09 t2086 hypothetical protein t2086 943 NP_805844.1 2.655355 92530 F10 t2110 hypothetical protein t2110 943 NP_805867.1 1.777306 92557 F11 t2245 citrate lyase subunit beta 943 NP_805987.1 1.243902 92694 F12 t2471 943 NP_806200.1 1.863203 92630 G01 t2623 hypothetical protein t2623 943 NP_806334.1 1.981972 92509 G02 t3431 phage baseplate assembly protein 943 NP_807094.1 2.35737 92425 G03 t3685 LysR family transcriptional regulator 943 NP_807321.1 1.304348 92609 G04 t4287 hypothetical protein t4287 943 NP_807885.1 3.520679 92389 G05 t4307 phage baseplate assembly protein 943 NP_807903.1 1.946978 92590 G06 t0282 transcriptional regulator 946 NP_804158.1 1.723044

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Product Information Sheet for NR-19544

Well Locus ORF Accession Average Depth Clone Description Position ID Length Number of Coverage 92710 G07 t0418 cysteine synthase B 946 NP_804287.1 3.531712 92481 G08 t2038 ABC transporter permease 946 NP_805801.1 1.82241 92433 G09 t2472 recombination associated protein 946 NP_806201.2 1.743129 surface presentation of antigens protein 92441 G10 t2793 946 NP_806494.1 1.255814 SpaO 92697 G11 t3863 glycyl-tRNA synthetase subunit alpha 946 NP_807482.1 1.61945 92733 G12 t4553 hypothetical protein t4553 946 NP_808131.1 1.505285 92729 H01 t0805 transcriptional regulator 949 NP_804647.1 1.821918 92554 H02 t1077 L,D-carboxypeptidase A 949 NP_804894.1 1.797682 92661 H03 t2609 transcriptional regulator 949 NP_806325.1 1.935722 92525 H04 t2894 hypothetical protein t2894 949 NP_806590.1 3.630137 92570 H05 t3174 carbohydrate kinase 949 NP_806853.1 1.806112 92717 H06 t3414 hypothetical protein t3414 949 NP_807077.1 2.148577 92478 H07 t4255 hypothetical protein t4255 949 NP_807853.1 3.508957 92593 H08 t4577 mrr restriction system protein 949 NP_808153.1 1.917808 UDP-3-O-[3-hydroxymyristoyl] N- 92466 H09 t0138 952 NP_804021.1 1.796218 acetylglucosamine deacetylase 92597 H10 t0284 2-dehydropantoate 2-reductase 952 NP_804160.1 1.460084 92461 H11 t0550 ribonuclease Z 952 NP_804407.1 1.685924 92677 H12 t0690 periplasmic protein 952 NP_804538.1 1.303571 1All information in this table was provided by J. Craig Venter Institute at the time of deposition.

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