MOLECULAR AND CELLULAR BIOLOGY

Volume 10 October 1990 No. 10

GENE EXPRESSION Functional Analysis of the Murine T-Cell Receptor I Enhancer and Characteris- tics of Its DNA-Binding Proteins. Junji Takeda, Alec Cheng, Fabienne Mauxion, Christopher A. Nelson, Rodney D. Newberry, William C. Sha, Ranjan Sen, and Dennis Y. Loh...... 5027-5035 Exon as well as Intron Sequences Are cis-Regulating Elements for the Mutually Exclusive Alternative Splicing of the 0 Tropomyosin Gene. Domenico Libri, Maria Goux-Pelletan, E. Brody, and Marc Y. Fiszman...... 5036-5046 Role of ot-Fetoprotein Regulatory Elements in Transcriptional Activation in Transient Heterokaryons. Brett T. Spear and Shirley M. Tilghman ...... 5047-5054 Gene Induction by Interferons: Functional Complementation between trans-Acting Factors Induced by Alpha Interferon and Gamma Interferon. Sudip K. Bandyopadhyay, Dhananjaya V. R. Kalvakolanu, and Ganes C. Sen ...... 5055-5063 Cloning, Sequence, and Photoregulation of al-1, a Carotenoid Biosynthetic Gene of Neurospora crassa. Thomas J. Schmidhauser, Frank R. Lauter, Vincenzo E. A. Russo, and Charles Yanofsky ...... 5064-5070 Mutations That Define the Optimal Half-Site for Binding Yeast GCN4 Activator Protein and Identify an ATF/CREB-Like Repressor That Recognizes Similar DNA Sites. Joan W. Sellers, Alice C. Vincent, and Kevin Struhl...... 5077-5086 Multiple Positive and Negative cis-Acting Elements Mediate Induced Arginase (CARl) Gene Expression in Saccharomyces cerevisiae. Ladislau Kovari, Roberta Sumrada, lulia Kovari, and Terrance G. Cooper...... 5087-5097 Functional Analysis of the Transcriptional Promoter for the CYPIAJ Gene. Keith W. Jones and James P. Whitlock, Jr...... 5098-5105 A Transcriptionally Active Form of TFIHC Is Modified in Poliovirus-Infected HeLa CeUs. Melody E. Clark and Asim Dasgupta ...... 5106-5113 Molecular Characterization of the Drosophila melanogaster Urate Oxidase Gene, an Ecdysone-Repressible Gene Expressed Only in the Malpighian Tubules. Lori L. Wallrath, Jean B. Burnett, and Thomas B. Friedman...... 5114-5127 The C6 Zinc Finger and Adjacent Amino Acids Determine DNA-Binding Speci- ficity and Affinity in the Yeast Activator Proteins LAC9 and PPR1. Michael M. Witte and Robert C. Dickson ...... 5128-5137 E4F and ATF, Two Transcription Factors That Recognize the Same Site, Can Be Distinguished Both Physically and Functionally: A Role for E4F in ElA trans Activation. Robert J. Rooney, Pradip Raychaudhuri, and Joseph R. Nevins...... 5138-5149 Characterization of Promoter Elements of an Interferon-Inducible Ly-6E/A Dif- ferentiation Antigen, Which Is Expressed on Activated T Cells and Hemato- poietic Stem Cells. Khuda Dad Khan, Glen Lindwall, Stephen E. Maher, and Alfred L. M. Bothwell...... 5150-5159 Identification of a Protein Altered in Mutants Resistant to Microtubule Inhibitors as a Member of the Major Heat Shock Protein (hsp7O) Family. Suhail Ahmad, Renu Ahuja, Thomas J. Venner, and Radhey S. Gupta...... 5160-5165

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Additional Intragenic Promoter Elements of the Xenopus 5S RNA Genes Up- stream from the TFIIIA-Binding Site. Hans Joerg Keller, Qimin You, Paul J. Romaniuk, and Joel M. Gottesfeld ...... 5166-5176 Purification and Characterization of a Novel Factor Which Stimulates Rat Ribo- somal Gene Transcription In Vitro by Interacting with Enhancer and Core Promoter Elements. Ji Zhang and Samson T. Jacob ...... 5177-5186 Molecular Cloning and Analysis of the scon-2 Negative Regulatory Gene of Neurospora crassa. John V. Paietta...... 5207-5214 Translation to Near the Distal End of the Penultimate Exon Is Required for Normal Levels of Spliced Triosephosphate Isomerase mRNA. Jiu Cheng, Mirjana Fogel-Petrovic, and Lynne E. Maquat ...... 5215-5225 REB1, a Yeast DNA-Binding Protein with Many Targets, Is Essential for Cell Growth and Bears Some Resemblance to the Oncogene myb. Qida Ju, Bernice E. Morrow, and Jonathan R. Warner ...... 5226-5234 Ribosomal Protein L30 Is Dispensable in the Yeast Saccharomyces cerevisiae. Diane M. Baronas-Lowell and Jonathan R. Warner ...... 5235-5243 cis- and trans-Acting Regulatory Elements of the Yeast URA3 Promoter. A. Roy, F. Exinger, and R. Losson ...... 5257-5270 A Novel Pathway for Alternative Splicing: Identification of an RNA Intermediate That Generates an Alternative 5' Splice Donor Site Not Present in the Pri- mary Transcript of AMPDI. Ikuo Mineo, Peter R. H. Clarke, Richard L. Sabina, and Edward W. Holmes ...... 5271-5278 DNA-Binding Protein Activated by Gamma Radiation in Human Cells. Surinder P. Singh and Martin F. Lavin ...... 5279-5285 The Leucine Zipper of c-Myc Is Required for Full Inhibition of Erythroleukemia Differentiation. Michael J. Smith, Denise C. Charron-Prochownik, and Edward V. Prochownik...... 5333-5339 Parameters That Govern the Regulation of Immunoglobulin 8 Heavy-Chain Gene Expression. Roland Tisch, Naomi Kondo, and Nobumichi Hozumi ...... 5340-5348 Characterization of the 47-Kilodalton Autosomal Chronic Granulomatous Disease Protein: Tissue-Specific Expression and Transcriptional Control by Retinoic Acid. Adam R. F. Rodaway, Carmel G. Teahan, Colin M. Casimir, Anthony W. Segal, and David L. Bentley ...... 5388-5396 Wild-Type V(D)J Recombination in scid Pre-B Cells. Eric A. Hendrickson, Mark S. Schlissel, and David T. Weaver ...... 5397-5407 GLI3 Encodes a 190-Kilodalton Protein with Multiple Regions of GLI Similarity. J. Michael Ruppert, Bert Vogelstein, Kristina Arheden, and Kenneth W. Kinzler ...... 5408-5415 N-myc Expression Switched Off and Class I Human Leukocyte Antigen Expres- sion Switched On after Somatic Cell Fusion of Neuroblastoma Cells. Rogier Versteeg, Carolien van der Minne, Arianne Plomp, Alice Sijts, Aad van Leeuwen, and Peter Schrier...... 5416-5423 Identification of rpo3O, a Vaccinia Virus RNA Polymerase Gene with Structural Similarity to a Eucaryotic Transcription Elongation Factor. Byung-Yoon Ahn, Paul D. Gershon, Elaine V. Jones, and Bernard Moss ...... 5433-5441 The Promoter of the Human Interleukin-2 Gene Contains Two Octamer-Binding Sites and Is Partially Activated by the Expression of Oct-2. Mark P. Kamps, Lynn Corcoran, Jonathan H. LeBowitz, and David Baltimore..... 5464-5472

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The Mouse c-rel Protein Has an N-Terminal Regulatory Domain and a C-Termi- nal Transcriptional Transactivation Domain. Paulina Bull, Kimberlin L. Morley, Merl F. Hoekstra, Tony Hunter, and Inder M. Verma ...... 5473-5485 Identffication and Functional Characterization of the Human T-Cell Receptor I3 Gene Transcriptional Enhancer: Common Nuclear Proteins Interact with the Transcriptional Regulatory Elements of the T-Celi Receptor at and ,1 Genes. Lisa R. Gottschalk and Jeffrey M. Leiden ...... 5486-5495 Upstream Activation and Repression Elements Control Transcription of the Yeast COXSb Gene. Martin R. Hodge, Kavita Singh, and Michael G. Cumsky 5510-5520 A 22-Amino-Acid Peptide Restores DNA-Binding Activity to Dimerization-Defec- tive Mutants of the Estrogen Receptor. Jacqueline A. Lees, Stephen E. Fawell, Roger White, and Malcolm G. Parker...... 5529-5531 Fos and Jun Cooperate in Transcriptional Regulation via Heterologous Activation Domains. Cory Abate, Daniel Luk, Elizabeth Gagne, Robert G. Roeder, and Tom Curran ...... 5532-5535 Induction of the Proto-Oncogene c-jun by Angiotensin II. Allen J. Naftilan, Gretchen K. Gilliland, Carol S. Eldridge, and Andrew S. Kraft ...... 5536-5540 Increased Expression and DNA-Binding Activity of Transcription Factor Spl in Doxorubicin-Resistant HL-60 Leukemia Cells. Flavia Borellini, Angelo Aquino, Steven F. Josephs, and Robert I. Glazer...... 5541-5547 The DNA Damage-Inducible Gene DIN] of Saccharomyces cerevisiae Encodes a Regulatory Subunit of Ribonucleotide Reductase and Is Identical to RNR3. Kevin Yagle and Kevin McEntee...... 5553-5557 Inhibition of c-fos Transcription and Phosphorylation of the Serum Response Fac- tor by an Inhibitor of Phospholipase C-Type Reactions. Gunnar Schalasta and Clemens Doppler ...... 5558-5561 Transcription Initiation Complexes and Upstream Activation with RNA Polymer- ase II Lacking the C-Terminal Domain of the Largest Subunit. Stephen Buratowski and Phillip A. Sharp...... 5562-5564 Localization of a Phytochrome-Responsive Element within the Upstream Region of Pea rbcS-3A. Philip M. Gilmartin and Nam-Hai Chua...... 5565-5568 RNA Conformational Requirements of Self-Cleavage of Hepatitis Delta Virus RNA. Huey-Nan Wu and Michael M. C. Lai ...... 5575-5579 The Promoter and First, Untranslated Exon of the Human Glucocorticoid Recep- tor Gene Are GC Rich but Lack Consensus Glucocorticoid Receptor Element Sites. Jian Zong, Javed Ashraf, and E. Brad Thompson...... 5580-5585 Ordered Splicing of Thymidine Kinase Pre-mRNA during the S Phase of the Cell Cycle. Jean M. Gudas, Glenn B. Knight, and Arthur B. Pardee...... 5591-5595

CELL GROWTH AND DEVELOPMENT Activation of p56kk through Mutation of a Regulatory Carboxy-Terminal Tyrosine Residue Requires Intact Sites of Autophosphorylation and Myristylation. Ninan Abraham and Andre Veillette...... 5197-5206 Regulation of Tubulin Levels and Microtubule Assembly in Saccharomyces cerevi- siae: Consequences of Altered Tubulin Gene Copy Number. Wendy Katz, Brant Weinstein, and Frank Solomon ...... 5286-5294 Phenotypic Consequences of Tubulin Overproduction in Saccharomyces cerevisiae: Differences between Alpha-Tubulin and Beta-Tubulin. Brant Weinstein and Frank Solomon...... 5295-5304

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Cross-Linking of T-Cell Surface Molecules CD4 and CD8 Stimulates Phosphoryla- tion of the kck Tyrosine Protein Kinase at the Autophosphorylation Site. Kunxin Luo and Bartholomew M. Sefton...... 5305-5313 Effect of a Dominant Inhibitory Ha-ras Mutation on Mitogenic Signal Transduc- tion in NIH 3T3 Cells. Hong Cai, J6zsef Szeberenyi, and Geoffrey M. Cooper ...... 5314-5323 Effect of a Dominant Inhibitory Ha-ras Mutation on Neuronal Differentiation of PC12 Cells. J6zsef Szeberenyi, Hong Cai, and Geoffrey M. Cooper ...... 5324-5332 Transformation of Mouse BALB 3T3 Cells by Enterobacterial Plasmid Misrepair Gene mucAB. Mariko Tosu and Hiroshi Tanooka...... 5359-5364 Conditional Immortalization of Human Thyroid Epithelial Cells: A Tool for Anal- ysis of Oncogene Action. David Wynford-Thomas, Jane A. Bond, Fiona S. Wyllie, Jorge S. Burns, E. Dillwyn Williams, Tania Jones, Denise Sheer, and Nick R. Lemoine ...... 5365-5377 A Small v-sis/Platelet-Derived Growth Factor (PDGF) B-Protein Domain in Which Subtle Conformational Changes Abrogate PDGF Receptor Interaction and Transforming Activity. Neill Giese, William J. LaRochelle, Mary May-Siroff, Keith C. Robbins, and Stuart A. Aaronson ...... 5496-5501 Frequent Mutations in the p53 Tumor Suppressor Gene in Human Leukemia T-Cell Lines. Jian Cheng and Martin Haas ...... 5502-5509 Retrovirus-Mediated Transfer of a Gene Encoding an Adenovirus Membrane- Associated Protein Induces Down Regulation of the Receptor for Epidermal Growth Factor. Brian L. Hoffman, Axel Ullrich, William S. M. Wold, and Cathleen R. Carlin ...... 5521-5524 Cell Type-Specific Mechanisms of Regulating Expression of the Ornithine Decar- boxylase Gene after Growth Stimulation. Mitchell S. Abrahamsen and David R. Morris ...... 5525-5528 Stoichiometry of Cellular and Viral Components in the Polyomavirus Middle-T Antigen-Tyrosine Kinase Complex. Seng H. Cheng, Pearl C. Espino, John Marshall, Robert Harvey, and Alan E. Smith ...... 5569-5574 Double-Stranded RNA Unwinding and Modifying Activity Is Detected Ubiqui- tously in Primary Tissues and Cell Lines. Richard W. Wagner, Christopher Yoo, Lawrence Wrabetz, John Kamholz, Jeffery Buchhalter, Nassef F. Hassan, Kamel Khalili, Seung U. Kim, Bice Perussia, F. Arthur McMorris, and Kazuko Nishikura ...... 5586-5590 Cloning and Sequencing of a New gro Transcript from Activated Human Mono- cytes: Expression in Leukocytes and Wound Tissue. Naoko lida and Gary R. Grotendorst ...... 5596-5599

CELL AND ORGANELLE STRUCTURE AND ASSEMBLY Farnesyl Cysteine C-Terminal Methyltransferase Activity Is Dependent upon the STE14 Gene Product in Saccharomyces cerevisiae. Christine A. Hrycyna and Steven Clarke ...... 5071-5076

CHROMOSOME STRUCTURE AND DYNAMICS Distribution of Mutations around Rearranged Heavy-Chain Antibody Variable- Region Genes. Gerald W. Both, Linda Taylor, Jeffrey W. Pollard, and Edward J. Steele...... 5187-5196

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Sequence Conservation and Structural Organization of the Glycerol-3-Phosphate Dehydrogenase Promoter in Mice and Humans. Babette Gwynn, Kimberly A. Lyford, and Edward H. Birkenmeier...... 5244 5256 Mapping of Structural and Transcription-Related Matrix Attachment Sites in the a-Globin Gene Domain of Avian Erythroblasts and Erythrocytes. G. Farache, S. V. Razin, J. Rzeszowska-Wolny, J. Moreau, F. Recillas Targa, and K. Scherrer...... 5349-5358 Z-DNA Formation in the Rat Growth Hormone Gene Promoter Region. Michael J. Thomas, Thomas M. Freeland, and Jeannine S. Strobl...... 5378-5387 A Transpositionally and Transcriptionally Competent Alu Subfamily. A. Gregory Matera, Utha Hellmann, and Carl W. Schmid...... 5424-5432 Drosophila Scaffold-Attached Regions Bind Nuclear Scaffolds and Can Function as ARS Elements in Both Budding and Fission Yeasts. Bruno Amati and Susan M. Gasser...... 5442-5454 Studies of an 800-Kilobase DNA Stretch of the Drosophila X Chromosome: Comapping of a Subclass of Scaffold-Attached Regions with Sequences Able to Replicate Autonomously in Saccharomyces cerevisiae. Christine Brun, Qi Dang, and Raymond Miassod...... 5455-5463 Evolutionary Origin of the U6 Small Nuclear RNA Intron. Claudia Reich and Jo Ann Wise ...... 5548-5552

ERRATA Analysis of Saccharomyces cerevisiae his3 Transcription In Vitro: Biochemical Support for Multiple Mechanisms of Transcription. Alfred S. Ponticelli and Kevin Struhl ...... 5600 A Regulatory Element Is Characterized by Purine-Mediated and Cell-Type- Specific Gene Transcription. Martin J. Walsh, Antonio Sanchez-Pozo, and Neal S. Leleiko...... 5600

Date of Issue: 19 September 1990