JOURNAL OF BACTERIOLOGY VOLUME 164 o DECEMBER 1985 * NUMBER 12 Simon Silver, Editor in Chief (1987) Robert H. Rownd, Editor (1990) Washington University June J. Lascelles, Editor (1989) Northwestern Medical School St. Louis, Mo. University of , Los Angeles Chicago, Ill. James D. Friesen, Editor (1987) Richard M. Losick, Editor (1988) Graham C. Walker, Editor (1990) University of Toronto Harvard University Massachusetts Institute of Toronto, Canada Cambridge, Mass. Technology C. Holt, Editor (1987) L. Nicholas Ornston, Editor (1987) Cambridge, Mass. The University of Texas Health Robert A. Weisberg, Editor (1990) Science Center, San Antonio New Haven, Conn. National Institute of Child Samuel Kaplan, Editor (1988) Health and University of Illinois, Urbana Development Bethesda, Md.

EDITORIAL BOARD David Apirion (1985) A. Eisenstark (1985) K. W. Knox (1985) Palmer Rogers (1987) Arthur I. Aronson (1985) Wolfgang Epstein (1987) Wil N. Konings (1987) Barry P. Rosen (1986) Stuart J. Austin (1987) David Figurski (1987) Jordan Konisky (1987) Harry Rosenberg (1,985) Frederick M. Ausubel (1986) Timothy J. Foster (1986) Dennis J. Kopecko (1987) Rudiger Schmitt (1986) Gad Avigad (1986) John D. Foulds (1985) Terry Krulwich (1987) June R. Scott (1987) Barbara Bachmann (1987) Masamitsu Futai (1985) Thomas G. Lessie (1985) Jane K. Setlow (1987) Manfred E. Bayer (1985) Ann K. Ganesan (1985) Lasse Lindahl (1987) Peter Setlow (1987) Margaret H. Bayer (1986) Robert Gennis (1985) Jack London. (1987) Lucille Shapiro (1986) Claire M. Berg (1986) Jane Gibson (1985) Paul S. Lovett (1987) Issar Smith (1987) Robert-W. Bernlohr (1985) E. Peter Greenberg (1985) Ben J. J. Lugtenberg (1986) Paul F. Smith (1985) Terry J. Beveridge (1985) Barry G. Hall (1985) Robert Macnab (1985) Brian G. Spratt (1985) Edwin Boatman (1986) R. E. W. Hancock (1987) Francis L. Macrina (1986) Catherine Squires (1987) I. D. J. Burdett (1985) Robert Haselkorn (1987) Ann Matthysse (1985) I. W. Sutherland (1985) Joseph- M. Calvo. (1987) Gerald L. Hazelbauer (1987) Sarah A. Mclntire (1986) Robert Switzer (1987) A. M. Chakrabarty (1986) Charles E. Helmstetter (1985) Larry McKay (1987) Alexander Tomasz (1986) Keith F. Chater (1985) Ulf Henning (1985) S. Mizushima (1985) James L. Van Etten (1985) Terence G. Cooper (1987) Charles L. Hershberger (1987) Edward A. Morgan (1987) Godfried D. Vogels (1987) John E. Cronan, Jr. (1986) Penny J. Hitchcock (1987) David Mount (1985) Barry Wanner (1987) Walter B. Dempsey (1986) Bruce Holloway (1985) R. G. E. Murray (1986) Bernard Weisblum (1986) Patrick Dennis (1985) Martha M. Howe (1987) Robert A. Niederman (1985) David Womble (1986) W. D. Donachie (1985) Karin Ihlet (1987) Patrick J. Piggot (1987) Henry C. Wu (1987) W. (1985) A. W. B. Johnston (1986) Barry Polisky (1987). Duane C. Yoch (1986) J. Allan Downie (1985) Robert J. Kadner (1985) Linda Randall (1987) Howard Zalkin (1985) Gerhart Drews (1985) Eva R. Kashket (1987) William S. Reznikoff (1985) David A. Dubnau (1986) Edward Kellenberger (1985) Marilyn Roberts (1986) Helen R. Whiteley, Chairman, Publications Board Linda M. Illig, Managing Editor, Journals Dennis G. Burke, Production Editor

The Journal of Bacteriology (ISSN 0021-9193), a publication of the American Society for , 1913 I St., NW Washington, DC 20006, is devoted to the advancement and dissemination offundamental knowledge concerning and other . Instructions to authors are published in the January issue each year; reprints are available from the editors and the Publications Department. The. Jouirnal is published monthly, and the twelve, numbers are divided into four volumes per year. The nonmember subscription price is $249 per year; single copies are $21. The member subscription price is $37 (foreign, $49 [surface rate]) per year; single copies are $7. Correspondence relating to subscriptions, reprints, defective copies, availability of back issues, lost or late proofs, disposition of submitted manuscripts, and general editorial matters should be directed to the ASM Publications Department, 1913 I St., NW, Washington, DC 20006 (phone: 202 833-9680). Claims fo'r missing issues from residents of the , Canada, and Mexico must be submitted within 3 months after publication of the issues; residents of all other countries must submit claims within 6 months of publication of the issues. Claims for issues missing because offailure to report an address change or for issues "missing from " will not be allowed. Second-class postage paid at Washington, DC 20006, and at additional mailing offices. POSTMASTER: Send address changes to Journal of Bacteriology, ASM, 1913 I St., NW, Washington, DC 20006. Made in the United States of America. Copyright © 1985, American Society for Microbiology. El fi'etM1;A A,J(L I Jrtf 1,; All Rights Reserved. The code at the top of the first page of an article in this journal indicates the copyright owner's consent that copies of the arti- cle may be made for personal use or for personal use of specific clients. This consent is given on the condition, however, that the copier pay the stated per-copy fee through the Copyright Clearance Center Inc., 21 Congress St., Salem, MA 01970, for copying beyond that permitted by Sections 107 and 108 of the U.S. Copyright Law. This consent does not extend to other kinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. Author Index

Ainsworth, Teresa, 1233 Garciarrubio, Alejandro, 1032 Maillet, Fabienne, 1200 Sato, Gihei, 977 Atlan, Daniele, 1376 Garnerone, Anne-Marie, 1200 Malamy, Michael H., 1248 Sawers, R. Gary, 1324 Gibson, Janet L., 1188 Mandecki, Wlodek, 1353 Schell, Maria G., 1301 Ballantine, Stuart P., 1324 Gilchrist, Carol A., 1004 Manson, Michael D., 1057 Shah, Vinod K., 1081 Barros, M. E. C., 1386 Gokul, Nanda K., 1049 Marrero, Robert, 1283 Shapiro, James A., 1171 Barroso, G., 1094 Goldman, Robert A., 1353 Maurer, Kick, 1064 Shen, Gwo-Jenn, 1153 Bassett, Carole L., 1362 Gots, Joseph S., 1288 Maurizi, Michael R., 1124 Silver, Simon, 983 Bastarrachea, Fernando, 1032 Gottesman, Susan, 1124 McGroarty, Estelle J., 1256 Singer, M. E., 1011, 1017 Batut, Jacques, 1200 Grisolia, Vincenza, 1317 Mendelson, Neil H., 1136, Sirotkin, Karl M., 1301 Berg, Claire M., 1350 Gunge, Norio, 1373 1141 Sjoberg, Britt-Marie, 1194 Bhandari, Basant, 1383 Gunsalus, Robert P., 1100 Mieschendahl, Martin, 1366 Sjogren, Agneta, 1278 Bialkowska-Hobrzanska, Misra, Tapan K., 983 Smith, Linda Tombras, 1088 Hanna, 1004 Haapasalo, Markus, 1278 Molin, S0ren, 1262 Snyder, Irvin S., 1071 Bjork, Glenn R., 1117 Hahne, Solveig, 1194 MouchEs, C., 1094 S0gaard-Andersen, Liselotte, Bohach, Gregory A., 1071 Haldenwang, William G., 1356 Muller-Hill, Benno, 1366 1262 Booth, Ian R., 1218, 1224 Halverson, Larry J., 1301 Song, Seung-dal, 1271 Borgia, Peter T., 1039, 1049 Hancock, Robert E. W., 1256 Nappo, Anna Guilia, 1317 Spitzer, Eric D., 994 Bove, J. M., 1094 Harms, Nellie, 1064 Natvig, Donald O., 1309 Stacey, Gary, 1301 Boxer, David H., 1324 Hartmann, Anton, 1271 Nelson, Kristin K., 1301 Stouthamer, Adrian H., 1064 Brill, Winston J., 1081 Higgins, Christopher F., 1218, Nicholas, D. J. D., 1383 Stuber, Dietrich, 1117 Bruni, Carmelo Bruno, 1317 1224 Sugisaki, Yuji, 1373 Bulthuis, Roel, 1294 Homma, Michio, 1370 Opgenorth, A., 1238 Burris, Robert H., 1271 Hovmoller, Sven, 1278 Tabita, F. Robert, 1188 Hyun, H. H., 1146, 1153, 1162 Paranchych, W., 1238 Takemura, Tomoaki, 1182 Cairney, John, 1218, 1224 Perombelon, Michel C. M., Tally, Francis P., 1248 Candresse, T., 1094 Idota, Yuji, 1182 1110 Tamura, Gakuzo, 1373 Carlomagno, Maria Stella, Ihara, Hayato, 1182 Peterson, Arnold A., 1256 Terzaghi, Betty, 1200 1317 lino, Tetsuo, 1370 Peterson, Bryan C., 1359 Thwaites, J. J., 1136 Caruthers, Marvin H., 1353 Imperial, Juan, 1081 Phillips, Gregory J., 1039, Trempy, Janine E., 1356 Chatterjee, Anadi N., 1337 Iritani, Sumiko, 1182 1049 Trisler, Patsy, 1124 Chu, Lien, 983 Ishiguro, Edward E., 1233, Phipps, B. M., 1332 Truchet, Georges, 1200 Covarrubias, Alejandra A., 1332 Platz, Anton, 1194 Trust, Trevor J., 1233, 1332 1032 Ishiguro, Naotaka, 977 Portalier, Raymond C., 1376 Tsukagoshi, Norihiro, 1182 Postma, Erik, 1294 Dahl, Michael K., 1057 Jones, Helen M., 1100 Potts, Malcolm, 1025 Udaka, Shigezo, 1182 Debelle, Frdddric, 1200 Powell, Bradford S., 1353 Ugalde, Rodolfo A., 1081 Denarid, Jean, 1200 Karamata, D., 1141 Denhardt, David T., 1004 Karlsson, Margareta, 1194 Ranta, Helena, 1278 Vasse, Jacques, 1200 de Vries, Gert E., 1064 Kay, William W., 1233, 1332 Ranta, Kari, 1278 Veltkamp, Eduard, 1064 de Vrij, Wim, 1294 Kessler, Andrew I., 1288 RayChaudhuri, Debabrata, Verma, Meera, 1381 Doker, Renate, 1211 Koch, H. Uwe, 1211 1337 Kogoma, Tokio, 1309 Rawlings, D. E., 1386 Wang, Ming-der, 1350 Egan, J. Barry, 1381 Robillard, Norman J., 1248 Weiss, Bernard, 994 Eriksson, Staffan, 1194 Konings, Wil N., 1294 Rokeach, Luis A., 1262 Kopec, Leslie K., 1283 Romero, David, 1032 Whalen, William A., 1350 Farr, Spencer Bryant, 1309 Kushner, Sidney R., 1362 Rosenberg, Charles, 1200 Woods, D. R., 1386 Farrants, George, 1278 Rownd, Robert H., 1359 Wroblewski, H., 1094 Favre, Didier, 1136, 1141 Laddaga, Richard, 983 Russell, Paul, 1301 Finlay, B. B., 1238 Lazzaroni, Jean Claude, 1376 Yamagata, Hideo, 1182 Finnerty, W. R., 1011, 1017 Lee, J. S., 1238 Saillard, C., 1094 Yamasaki, Makari, 1373 Fischer, Werner, 1211 Le6n, Patricia, 1032 Sakaguchi, Kenji, 1373 Yasbin, Ronald E., 1283 Forbes, Kenneth J., 1110 Lindstrom, Par H. R., 1117 Sasaki, Takuji, 1182 Frost, L. S., 1238 Lounatmaa, Kari, 1278 Sasatsu, Masansori, 983 Zeikus, J. G., 1146, 1153, 1162 ACKNOWLEDGMENT

The following have served as invited special reviewers for the Journal during 1985, and their help is greatly appreciated.

Mark Achtman H. D. Caldwell Henry L. Ehrlich M. Carolyn Hardegree Lloyd M. Kozloff Sankar Adhya David H. Calhoun S. Dusko Ehrlich Simon S. J. Hardy V. Krishnapillai Nina Agabian Ercole Canale-Parola Eric Eisenstadt A. R. Harker A. Kroger Mary M. Allen Frank Cannon Barry I. Eisenstein Franklin M. Harold Herbert E. Kubitschek Karlheinz Altendorf Marian Carlson George Elkan William A. Haseltine Paul Kuempel Sidney Altman Bruce Carlton David J. Ellar J. Woodland Hastings Peter L. Kuempel Bruce N. Ames George M. Carman Claudine Elmerich Thomas P. Hatch Howard Kuramitsu Giovanna F. Ames Michael Cashel Bert Ely Youssef Hatefi Donn J. Kushner Andrew A. Archibald L. L. Cavalli-Sforza Thomas F. Emery Stanley Hattman Sidney Kushner Wilfred N. Arnold Roberto T. F. Celis Jerald Ensign Robert W. Hedges Sydney G. Kustu Arthur Aronson Glenn Chambliss Michael Esposito L. Heffernan Kyuno-Joo Kwon-Chung Daniel J. Arp Michael Chandler Joseph 0. Falkinham George Hegeman Sanford A. Lacks Stanley Artz Shing Chang Hanke Hennecke Richard Laddaga Alan Atherly A. K. Chatterjee J. G. Ferry Dennis Henner A. Laduzinski Tov Atlung Barry K. Chelm Robert H. Fillingame M. Herdman J. Oliver Lampen Fred Ausubel Oscar P. Chilson Richard A. Finkelstein Christopher F. Higgins Arthur Landy James Baddiley Mary-Dell Chilton Richard A. Firtel Michael Higgins Janos Lanyi Michael Bagdasarian Joanne Chory Robert Fisher James A. Hoch K. Gordon Lark T. 0. Baldwin M. Christman Susan Fisher L. V. Holdeman John M. Larkin Clinton E. Ballou Ronald Cihlar Cecil Forsberg 1. B. Holland Richard A. Laursen Albert Balows Vincent Cirillo Dan G. Fraenkel Randall K. Holmes Andree Lazdunski Richard Baltz Alvin J. Clark Ernst Freese Magnus Hook Edward R. Leadbetter L. Baresi Brian F. C. Clark Irwin Fridovich P. Hooykaas Donald LeBlanc Eugene M. Barnes Don Clewell David Friedman Jim Hopper Terrence Leighton Louis S. Baron Royston C. Clowes G. Fuchs David Hopwood Sally Leong Paul Baumann J. G. Cobley R. Clinton Fuller Martha Howe Rodney L. Levine S. T. Bayley Stanley N. Cohen Jonathan Gallant R. C. Huffaker Barry Levinson June D. Beggs M. D. Collins Jeffrey F. Gardner E. J. L. Hughes Stuart Levy Marlene Belfort H. Edward Conrad Costa P. Georgopoulos F. Marion Hulett Margaret Lieb M. F. Bennett R. Conrad Philipp Gerhardt John landolo Susan W. Liebman R. Bentley Stephen Cooper William Ghiorse John L. Ingraham Edward C. C. Lin Douglas E. Berg Pascale Cossartllo Bijan K. Ghosh Lonnie 0. Ingram Thomas Lindahl Howard Berg David Cotter Ronald J. Gibbons Masayori Inouye Alf A. Lindberg John E. Beringer Donald L. Court Michael Glaser Edward E. Ishiguro S. Lindow Michael Berman C. D. Cox A. Glazer Venkatram N. Iyer Barry R. Bochner William A. Cramer Andrew Glenn N. Jacobs James A. Lippincott Daniel Boone Irving P. Crawford Walter Godchaux William Jacobs John W. Little Winfried Boos Jorge Crosa Werner Goebel Judith Jaehning Paul Loach Peter Borgia Laszlo N. Csonka Larry Gold Karen S. Jakes Sharon Long Leendert Bosch Roy Curtiss III Marvin Gold John R. Johnson Paul Ludden Keith A. Bostian Stanley Dagley Fred Goldberg Gerald C. Johnston Donald R. Leuking Michael Botchan Albert E. Dahlberg Robert C. Goldman Elizabeth Jones Salvador E. Luria J. Botsford Frederick W. Dahlquist Robert C. Goldnar 0. T. G. Jones Joan E. Lusk Kenneth Bott H. Dailey Sol H. Goodgal Clarence 1. Kado Jospeh F. Lutkenhaus B. Bowien Lolita Daneo-Moore Emil Gotschlich A. Dale Kaiser Carolyn H. MacGregor Barry J. Bowman Richard Darveau Susan Gottesman Olle H. Karlstrom George Mackie Marjorie Brandriss Wayne Davies G. Gottschalk Dennis Kasper M. Madigan Volkmar Braun Donald Dean Jay D. Gralla D. P. Kelly Boris Magasanik Hans Bremer F. DeMatthis Ron Greene J. T. Keltjens Paul T. Magee Patrick J. Brennan Jean Denarie E. Gregory Christina Kennedy Robert J. Maier B. A. Bridges Raymond Devoret Carol A. Gross Eugene Kennedy Michael H. Malamy Winston J. Brill Peter Devreotes Alan Grossman David E. Kennell Peter C. Maloney Stuart Brody A. M. Dhople John R. Guest G. E. Kenny Stanley Maloy G. M. Brown David N. Dietzler Donald G. Guiney, Jr. H. Gobind Khorana Jack Maniloff Nigel L. Brown M. J. Dilworth Richard 1. Gumport Amar Klar John Mankowitz James L. Brunton Walter J. Dobrogosz Robert P. Gunsalus Nancy E. Kleckner Paul A. Manning Lawrence E. Bryan Raymond N. Doetsch Mark Guyer Bruce C. Kline Colin Manoil Christine Buchanan Roy Doi T. Hackstadt Arthur L. Koch M. G. Marinus Robert B. Buchanan Thomas F. Donahue Scott Hagedorn Tokio Kogoma Alvin Markovitz H. R. Buckley M. I. Donnelly Ian R. Hamilton P. E. Kolattukudy Julius Marmur Lee A. Bulla, Jr. Reed C. Doten Phillip C. Hanawalt Richard Kolodner Barry L. Marrs Robert B. Burchard William Dowhan F. Hansen Adam Konderosi Lorraine Marsh Ronald J. Doyle Leonard E. Martinson Vickers Burdett J. Norman Hansen Wil N. Konings Howard H. Drake George A. Marzlut Bussey Gary Dunny T. Hansen Dennis Kopecko Al Matheson Ronald A. Butow Martin Dworkin Richard S. Hanson Daniel E. Koshland Abdul Matin Benjamin R. Byers Dan Dykhuizen Klaus Hantke Stephen Kowalczykowski Stephen J. Mattingly Felipe C. Cabello Charles Earhart S. Harayama Thomas R. Kozel W. R. Mayberry Barry C. McBride Fritz Orskov Lawrence 1. Rothfield Magdeleine So R. Uffen William McClure Mary J. Osborn Marjorie Russell Christopher Sommerville Agnes Ullman Ronald N. McElhaney Gary Ostroff Charles L. Rutherford Abraihiam L. Sonenshein C. Van der Drift Kevin McEntee David Ow Hairold Sadoff K. Sowers David Vasquez Al McEwan Sunil Palchaudhuri Milton H. Saier. Jr. Philip F. Sparling Karen Vavra Estelle McGroarty llkka Palva I. Salit Ian D. Spenser H. Veldkamp Calvin S. McLaughlin William Paranchych M. J. Salton John L. Spudich Mernia R. Villarejo John Mekalanos Mary Lou Pardue Abigail A. Sailyers Thressa C. Stadtman Dennis Voelker Neil H. Mendelson David Paretsky Nilima Sarkar Andrew L. Stiaehelin J., Wall Mathew Meselson James T. Park Scarborough Frainklin W. Stahl Richard Warburg Richard J.. Meyer Jack Parker Charles Schatchtele Gary Steacy Jonath'an R. Warner Charles G. Miller John S. Parkinson Harry Schachter Deborah Steege J. Mark Weber Jim Miller Leo W. Parks Julius Schachter A. Sterkenburg Michatel Weber Robert W. Miller Henry P. Piulus Nat L. Sternberg Joel H. Weiner Dallis Mills Shelly Payne Alan T. Schauer Stanley E. Stevens. Jr. George M. Weinstock Tapan K. Misra W. J. Payne Prndy Schekman Roselynn Stevenson Emilio Weiss Peter Model Nancy Pearson Melvin Schindler Murray Stewart Rodney A. Welch Paul Modrich John Peer. Jr. H.-G. Schlegel Valley Stewart Carl A. Westby Marc van Montagu Janice Pero Robert F. Schleif David 1. Stirling Helen R. Whiteley Walter E. C. Moore Michael C. M. Perombe- David Schlessinger Jeffry B. Stock C. J. Whitfield Charles P. Morgan Ion Carl D. Schnaitman A. Stouthamer Reed B. Wickner Edvard A. Morgan Jerome J. Perry J. Schnell Mark D. Stowers Brian Wilkinson David Morrison Robert D. Perry Janet Schottel Jeff Strathern Jim C. Williams L. E. Mortenson Paul Phibbs V. L. Schramm Uldis Streips Luther S. Williams Robert K. Mortimer A. T. Phillips Ira Schwartz Wolfgiang Strittmatter Palmer Williams Robert P. Mortlock George Phillips John Scott Jack L. Strominger Peter A. Williams B. Muller-Hill R. A. Pieringer Irwin H. Segal Anne 0. Summers Peter H. Williams Agnete Munch-Petersen Terry Platt Mark S. Shanley Martin Sumner-Smith Gail Willsky Ellen Murphy Jeanne S. Poindexter Fred Sherman John C. Swanson Herbert H. Winkler John R. Murphy D. J. Pollack Louis A. Sherman Richiard B. Sykes Malcolm E. Winkler Noreen E. Murray C. Pootjes R. A. Shilperoort F. Robert Tabita Kim S. Wise R. Musso Daniel Portnoy Larence J. Shimkets C. Tabor Carl R. Woese Carol Muster Kaithleen Postle Gerald D. Shockman M. Takagi John Wohlhieter Atsushi Nakazawa Louise Prakash Steven Short J. Takemoto Richard Wolf Teruko Nakazawa Robert H. Pritchard Robert G. Shulman De(an Tlylor R. Wolfe Howard A. Nash Ann Progukke-Fox H. A. Shuman D. Tempest M. J. Wolin Frederick C. Neidhardt Anthony Pugsley Eli Siegel Ethel S. Tessman Janet M. Wood Joseph Neilands Miroslav Radman Ethan R. Signer R. Thauer David R. Woods William R. Nes Christian Raetz Thomas Silbhavy J. Thayer John L. Woolford Eugene W. Nester K. V. Rajagopalan Richard P. Silver Teresa Thiel Btarbara E. Wright Francis C. Neuhaus G. H. Rank Michael Silverman Christopher M. Thomas Ichiro Yamashita Halina Neujahr N. N. Rao Melvin Simon Curtis B. Thorne Ka-Leung Ngai Georges Rapoport Robert D. Simoni Jeremy Thorner Ronald E. Yasbin Hiroshi Nikaido K. N. Raymond Robert Simons Kenneth N. Timmis Elton T. Young H. G. Nimmo R. H. Reed Andrew Simpson Thea Tlsty Michael Young Dale Noel Henry C. Reeves A. T. Sinskev Alexander Tomiasz Philip Youngman Richard P. Novick Sydney Rittenberg H. Tokuda Charles 0. Rock Nigel Skipper Jun-Ichi Tomizawa Trakatshi Yura Alois Nowotny Randy Roehl Carolyn W. Slaymain Annamaria Torriani Stanley A. Zahler William Nunn Howard J. Rogers Ralph A. Slepecky Adriane C. Toussaint Norton D. Zinder Gerald O'Donovan Eliora Z. Ron Alan Sloma Thomas A. Trautner S. Zinder J. Oelze Antonio Romano Derek Smith Trevor Trust Peter Zuber Joel Oppenheim John R. Roth Jeffrey Smith Ollie H. Tuovinen David Zusman AUTHOR INDEX VOLUME 164

Agarwal, Arun K., 757 Cabrera-Juarez, Emiliano, Egelhoff, T. T., 591 Halvorson, H. O., 302 Aguilar, 0. Mario, 245 525, 535 Ellar, David J., 192 Hancock, Robert E. W., 1256 Aiba, Hiroji, 872 Cairney, John, 1218, 1224 Elwell, Lynn, 470 Handa, Avtar K., 473 Qiba, Shuichi, 85 Calder, Kathleen M., 401 Ely, Susan, 484 Hanus, F. Joe, 187 Ainsworth, Teresa, 1233 Campbell, Lee Ann, 461 Emmerson, Peter T., 653 Harford, S., 114 Albritton, William L., 525 Candido, E. P. M., 957 Engwall, Judy K., 390 Harker, Alan R., 187 Aldea, Martf, 487, 950 Candresse, T., 1094 Eriksson, Staffan, 1194 Harms, Etti, 207, 217 Ambulos, Nicholas P., Jr., Carlino, Anthony, 223 Evans, Harold J., 187 Harms, Nellie, 1064 696 Carlomagno, Maria Stella, Ewing, David F., 8% Hartmann, Anton, 1271 Arora, Yogesh K., 811 1317 Hattman, Stanley, 932 Atlan, Daniele, 1376 Caruthers, Marvin H., 1353 Falkow, Stanley, 321 Heffernan, Laurel, 717, 831 Attfield, Paul V., 276 Chakrabarty, A. M., 516 Farr, Spencer Bryant, 1309 Helinski, Donald R., 446 Chambers, Philip, 653 Farrants, George, 1278 Henning, Ulf, 539 Bakker, Evert P., 904 Changler, Carol J., 674 Favre, Didier, 1136, 1141 Higgins, Christopher F., 1218, Ballantine, Stuart P., 1324 Chapon, Christine, 633, 639 Fayat, Guy, 63 1224 Baltz, Richard H., 944 Charles, I. G., 114, 123 Ferguson, Stuart J., 823 Himeno, Michio, 872 Banai, Menachem, 626 Chatterjee, Anadi N., 1337 Ferone, Robert, 470 Hirota, Yukinori, 456 ,3arclay, Raymond, 8% Chatterjee, Arun K., 14, 390 Fiebig, Klaus, 95 Hoekstra, Wiel P. M., 563 Barran, L. R., 410 Chu, Lien, 983 Finch, Paul W., 653 Homma, Michio, 1370 Barron, Anne, 674 Churchward, G., 922 Finlay, B. Brett, 571, 1238 Hovmoller, Sven, 1278 Barros, M. E. C., 1386 Clark, Virginia L., 461 Finnerty, W. R., 1011, 1017 Hsu, Jung-Hsin, 207, 217 Barroso, G., 1094 Cleary, P. Patrick, 600 Fischer, Werner, 1211 Huffman, Gary A., 33, 723 Barton, Larry L., 316 Clewell, Don B., 782 Forbes, Kenneth J., 1110 Husemann, Manfred, 954 ,Bassett, Carole L., 1362 Close, T. J., 774 Fromant, Michel, 63 Hussain, Musaddeq, 223 Bassford, Philip J., Jr., 665 Coffell, Richard, 911 Frost, L. S., 1238 Hutchinson, C. Richard, 425 ljastarrachea, Fernando, 1032 Collmer, Alan, 51 Fuerst, C. R., 960 Hyun, H. H., 1146, 1153, 1162 Batut, Jacques, 1200 Comeau, Dorothy E., 578 Fukui, Sakuzo, 769 Beachey, Edwin H., 350 Coomes, Marguerite Wilton, Furuichi, Teiichi, 270, 914 landolo, John J., 288 Beezley, Ann, 646 646 Idota, Yuji, 1182 Benson, Fiona E., 276 Cooperider, Jennifer S., 501 Ganeshkumar, N., 255 Ihara, Hayato, 1182 Berg, Claire M., 1350 Costenbader, Carla J., 866 Garberi, Juan C., 1 lino, Tetsuo, 1370 Berkelman, T., 918 Covarrubias, Alejandra A., Garciarrubio, Alejandro, 1032 Ikenaka, Kazuhiro, 578 Bernlohr, Robert W., 938 1032 Gardiner, William E., 762 Imanaka, Tadayuki, 85 Betlach, Mary C., 414 Cramer, W. A., 237 Garnerone, Anne-Marie, 1200 Imperial, Juan, 1081 Bhandari, Basant, 1383 Craven, Rebecca, 544 Gay, P., 918 Ingram, Lonnie O., 173 Bialkowska-Hobrzanska, Gentz, Reiner, 70 Inokuchi, Kaoru, 585 Hanna, 1004 Dahl, Michael K., 1057 George, Susan E., 866 Inouye, Masayori, 270, 578, Bishop, Christopher J., 367 Dahlbeck, Doug, 359 Ghosh, Arati, 107 914 Bishop, L. J., 237 Darzins, Aldis, 516 Ghosh, Bijan K., 107 Inouye, Sumiko, 270, 914 Bjes, E. S., 237 Davidson, Victor L., 237, 941 Gibson, Janet L., 1188 Iritani, Sumiko, 1182 Bjork, Glenn R., 1117 Dawson, Chandler R., 344 Giffard, Philip M., 904, 972 Ishiguro, Edward E., 861, Blanchin-Roland, Sylvie, 63 Debelle, Frederic, 1200 Gilchrist, Carol A., 1004 1233, 1332 Blanquet, Sylvain, 63 de la Campa, Adela G., 487, Glaser, Gad, 19 Ishiguro, Naotaka, 977 Blaut, Michael, 95 950 Gmeiner, Jobst, 741 Itoh, Masao, 585 Blumenthal, Robert M., 501 de Macario, Everly Conway, Gober, James W., 929 Bogomolni, Roberto A., 282 1 Goguen, Jon D., 704 Jackson, J. Barry, 823 Bohach, Gregory A., 1071 DeMoss, John A., 25 Gokul, Nanda K., 1049 Jayaswal, Radheshyam K., Bone, Eileen J., 192 Denarie, Jean, 1200 Golden, Kendra J., 938 473 Booth, Ian R., 904, 972, 1218, den Blaauwen, J. L., 477 Goldman, Robert A., 1353 Johnson, Barbara L., 925 1224 D'Enfert, C., 633 Gonda, Matthew A., 626 Johnson, E. A., 479 Borgia, Peter T., 1039, 1049 Denhardt, David T., 1004 Gordon, Milton P., 33, 723 Jones, Benjamin L., 757 B3oschwitz, H., 302 De Siervo, August J., 684 Gossett, Debra, 605 Jones, Donald, 957 Bove, J. M., 1094 de Vries, Gert E., 1064 Gots, Joseph S., 1288 Jones, Helen M., 1100 Bowien, Botho, 954 de Vrij, Wim, 1294 Gottesman, Susan, 1124 Jurgens, Uwe J., 384 Boxer, David H., 1324 DeWitt, Janet P., 969 Gottschalk, Gerhard, 95 Boyer, Herbert W., 414 Dhundale, Anilkumar R., 914 Gowrishankar, J., 434 Kaboev, Oleg K., 421, 878 Dingmarn, Douglas W.,155 Greenberg, E. P., 45 C. 918 Bremer, H., 922 Doi, Roy H., 550 Kado, I., 774, Bressan, Ray A., 473 Greenfield, Anthony J., 823 Kaplan, Samuel, 181 Doker, Renate, 1211 Gregory, Susan A., 501 245 Brill, Winston J., 1081 Dooley, J. S. G., 263 Kapp, Dieter, Bromfield, E. S. P., 410 Grisolia, Vincenza, 1317 Karamata, D., 1141 Dopazo, Ana, 950 Guan, Tinglu, 107 Karamata, Dimitri, 130 Bron, Sierd, 201 Dosch, Susan F., 611 Guerrero, Ricardo, 749 Brookfield, J. F. Y., 114 Karlsson, Margareta, 1194 Douglas, C. J., 102 Gunge, Norio, 1373 Kashket, Eva R., 929 Bruni, Carmelo Bruno, 1317 Drlica, Karl, 947 Gunsalus, Robert P., 1100 Kaufman, Joshua D., 6% Bryant, Donald A., 659 Dubbs, James M., 659 Guthrie, Ellen P., 510 Kawamukai, Makoto, 872 Buckman, Carol, 836 Dubin, Robert A., 605 Kawamura, Fujio, 550 Bueno, Raphael, 816 Duncan, Bruce K., 689 Haapasalo, Markus, 1278 Kay, William W., 1233, 1332 Bujard, Hermann, 70 Dunlap, Paul V., 45 Hahne, Solveig, 1194 Keil, Heinrich, 887 Bulthuis, Roel, 1294 Haldenwang, William G., 1356 Keil, Susanne, 887 Burris, Robert H., 1271 Egan, J. Barry, 1381 Halverson, Larry J., 1301 Keister, Donald L., 757 ii AUTHOR INDEX J. BACTERIOL.

Kessler, Andrew I., 1288 McEvoy, James L., 390 Pfeifer, Felicitas A., 414 Schultz, Terrie, 674 Keynan, A., 302 McEwan, Alastair G., 823 Phillips, Gregory J., 1039, Schwartz, M., 633 Keyte, J. W., 123 McGroarty, Estelle J., 1256 1049 Schwartzbach, Steve D., 762 Kirby, John R., 802 Mechulam, Yves, 63 Phipps, B. M., 1332 Segel, Irwin H., 674 Kishimoto, Jiro, 872 Meganathan, R., 911 Pickup, Roger W., 887 Setlow, Jane K., 525, 535 Klemm, Dwight J., 316 Mellot, Patrice, 63 Plateau, Pierre, 63 Setzer, Paulette Y., 344 Klintworth, Rolf, 954 Melton, Thoyd, 866 Platz, Anton, 1194 Shah, Vinod K., 1081 Knauf, V. C., 723 Mendelson, Neil H., 1136, Plotnick, Edwig A., 611 Shapiro, James A., 1171 Koch, H. Uwe, 1211 1141 Portalier, Raymond C., 1376 Shaw, D. H., 263 Kogoma, Tokio, 1309 Michaelis, S., 633 Porter, Ronald D., 659 Shaw, Jay H., 782 Kolling, Ralf, 310 Michels, Corinne A., 605 Postma, Erik, 1294 Shaw, W. V., 114, 123 Komano, Tohru, 872 Mieschendahl, Martin, 1366 Postma, P. W., 477 Shen, Gwo-Jenn, 1153 Konings, Wil N., 1294 Milde, Karin, 741 Potts, Malcolm, 1025 Sherburne, Richard, 338 Kopec, Leslie K., 1283 Milner, Y., 302 Powell, Bradford S., 1353 Shibata, Hitoshi, 762 Kothary, Rashmikant K., 957 Misra, Tapan K., 983 Pruss, Gail J., 947 Shoemaker, Nadja B., 510 Kramer, Cornelia, 539 Mitchell, Beverly K., 646 Pugsley, A. P., 633 Short, Helen B., 461 Kroll, Rohan G., 904 Mizuno, Takeshi, 797 Piihler, Alfred, 245 Silver, Simon, 983 Kushner, Sidney R., 1362 Mizushima, Shoji, 585, 797 Puvanesarajah, V., 102 Singer, M. E., 1011, 1017 Kusser, Wolfgang, 861 Molin, S0ren, 1262 Singer, Sara, 470 Kuziakina, Tamara I., 421, Mongkolsuk, Skorn, 6% Rabi,Tavassa, 25 Singh, Satya P., 367 878 Montie, Thomas C., 544 Raibaud, Olivier, 639 Sinha, Ramesh C., 811 Moore, L. W., 723 Randall, Linda L., 712 Sirotkin, Karl M., 1301 Laddaga, Richard, 983 Morgan, Michael K., 14 Ranhand, Jon M., 626 Sjoberg, Britt-Marie, 1194 Lallier, R., 263 Morona, Renato, 539 Ranta, Helena, 1278 Sjogren, Agneta, 1278 Lambert, David H., 659 Morris, E. Jane, 255 Ranta, Kari, 1278 Smillie, Lawrence B., 571 Lambert, Grant R., 187 Mosig, Gisela, 932 Rasmussen, Beth A., 665 Smith, C. Jeffrey, 294, 466 Lampen, J. Oliver, 223 Mouches, C., 1094 Ratledge, Colin, 8% Smith, Hilde, 201 Lascelles, June, 401 Muller, Volker, 95 Rawlings, D. E., 1386 Smith, Linda Tombras, 1088 Lazzaroni, Jean Claude, 1376 Muller-Hill, Benno, 1366 Ray, Paul H., 665 Smith, Michael R., 618 LeBlanc, Donald J., 626 Muren, Eva M., 712 RayChaudhuri, Debabrata, Smith, Thomas E., 646 Le Coq, D., 918 Mutschler, Ann, 525 1337 Snyder, Irvin S., 1071 Lee, Chia Yen, 288 Razin, Aharon, 19 S0gaard-Andersen, Liselotte, Lee, J. S., 1238 Nappo, Anna Guilia, 1317 Razin, Shmuel, 19 1262 Lee, Younghoon, 556 Natvig, Donald O., 1309 Re, Edward, 674 Sonenshein, Abraham L., 155 Lei, Shau-Ping, 717, 834 Needleman, Richard B., 605 Renosto, Franco, 674 Song, Seung-dal, 1271 Le6n, Patricia, 1032 Nelson, Kristin K., 1301 Riggsby, W. Stuart, 7 Sousa, Jose M. Fernandez, Lequerica, Juan L., 618 Nester, Eugene W., 33, 102, Robillard, Norman J., 1248 379 Leung, Judith O., 611 723 Rock, Charles O., 136 Spanier, Jonathan G., 600 Levine, R. L., 479 Ng, Lai-King, 338 Roeder, David L., 51 Spears, P. A., 321 Lewis, D. M., 410 Nicholas, D. J. D., 1383 Rogers, Peter J., 367 Spikes, Deborah, 525 Li, Songfeng, 25 Nicholas, Robert A., 456 Rokeach, Luis A., 1262 Spitzer, Eric D., 994 Lin, E. C. C., 479 Nichols, Barbara A., 344 Romero, David, 1032 Spudich, John L., 282 Lin, Hun-Chi, 717, 831 Nogami, Takako, 797 Rosenberg, Charles, 1200 Stacey, Gary, 102, 1301 Lindow, Steven E., 359 Nur, Israel, 19 Rosenkrantz, Mark S., 155 Stalon, Victor, 882 Lindstr6m, Par H. R., 1117 Rottem, Shlomo, 19 Staskawicz, Brian J., 359 Liss, Laura R., 925 Offermann, Paul V., Jr., 611 Rowland, Geoffrey C., 904, Steinmetz, M., 918 Lloyd, Robert G., 276, 836 Ohnuki, Tetsuo, 85 972 Stemnberg, Nat, 490 Long, S. R., 591 Oliver, Donald B., 925 Rownd, Robert H., 1359 Stevens, Audrey, 57 Lother, Heinz, 310 Opgenorth, A., 1238 Russell, Paul, 1301 Stevens, S. Edward, Jr., 659 Lounatmaa, Kari, 1278 Ordal, George W., 802 Stewart, Lorna M. D., 904 Lovett, Paul S., 696 Orndorff, Paul E., 321 Saillard, C., 1094 Stewart, Valley, 731 Luchkina, Ludmila A., 421, Orser, Cindy, 359 Sakaguchi, Kenji, 1373 Stiles, Michael E., 338 878 Oshima, Yasuji, 964 Salnikow, Johann, 954 Stirewalt, Veronica L., 659 Osman, Yehia A., 173 Salyers, Abigail A., 510 Stonesifer, Jill, 944 Macario, Alberto J. L., 1 Oyaizu, H., 230 Sanchez, Flora, 379 Stouthamer, Adrian H., 1064 Macdonald, Paul M., 932 Oyaizu, Y., 230 Sargent, Michael G., 192 Streeter, John G., 78 MacGregor, Carolyn H., 665 Strominger, Jack L., 456 Sarnow, Elke, 741 Stuber, Dietrich, 1117 Madonna, M. Jane, 223 Pahel, Greg, 816 Sasaki, Takuji, 1182 Magasanik, Boris, 816 Panasenko, Sharon M., 495 Subrahmanyam, C. S., 207 Sasatsu, Masansori, 983 Sugisaki, Yuji, 1373 Maillet, Fabienne, 1200 Pang, Frankye, 344 Sastry, Parimi A., 571 Malamy, Michael H., 1248 Panopoulos, Nickolas J., 359 Sundberg, Steven A., 282 Sato, Gihei, 977 Suzuki, Hideho, 456 Mandecki, Wlodek, 1353 Paranchych, William, 571, R. 1324 Manson, Michael D., 1057 1238 Sawers, Gary, Swinton, David, 932 Marquez, Gabriel, 379 Helen 802 Schaefer, Olivier, 130 Szyf, Moshe, 19 Parker, M., 321 Marrero, Robert, 1283 Pas, Evelien, 331 Schauer, David, Martfnez-Salas, Encarnaci6n, Pasloske, Brittan L., 571 Schell, F. M., 102 Tabita, F. Robert, 147, 1188 487 Pearlstone, Joyce R., 571 Schell, Maria G., 1301 Tai, Shih-Peng, 181 Marugg, Joey D., 563 Pedr6s-Ali6, Carlos, 749 Schippers, Bob, 563 Tait, R. C., 774 Mas, Jordi, 749 Peet, R., 723 Schlaeppi, Jean-Marc, 130 Takano, Yutaka, 769 Maurer, Kick, 1064 Pdrombelon, Michel C. M., Schlagman, Samuel, 932 Takemura, Tomoaki, 1182 Maurizi, Michael R., 1124 1110 Schmid, Ernst N., 397 Tally, Francis P., 1248 Mazer, Jonathan, 674 Peterson, Arnold A., 1256 Schmidhauser, Thomas J., 446 Tamai, Yukio, 964 McBride, Barry C., 255 Peterson, Bryan C., 1359 Schmidt, Francis J., 556 Tamura, Gakuzo, 1373 VOL. 164, 1985 AUTHOR INDEX iii

Taschner, Peter E. M., 331 Unger, L., 723 Weaver, Keith E., 147 Wolfe, Ralph S., 165 Taylor, Brian H., 33 Utsumi, Ryutaro, 872 Weber, J. Mark, 425 Woods, D. R., 1386 Taylor, Diane E., 338 Weckesser, Jurgen, 384 Wright, Andrew, 484 Terzaghi, Betty, 1200 Vallari, David S., 136 Weisbeek, Peter J., 563 Wroblewski, H., 1094 Thwaites, J. J., 1136 Vanags, R. I., 516 Weisburg, W. G., 230 Wyke, Anne W., 192 Todd, John A., 192 van Driel, Roel, 143 Weiss, Bernard, 994 Toh-e, Akio, 964 van Ophem, Peter, 143 Wetzstein, Heinz G., 823 Tormo, Antonio, 487, 950 van Spanje, Martin, 563 Whalen, William A., 1350 Yamagata, Hideo, 1182 Trempy, Janine E., 1356 Vasse, Jacques, 1200 White, Frank F., 33 Yamasaki, Makari, 1373 Trisler, Patsy, 1124 Veltkamp, Eduard, 1064 Whitman, William B., 165 Yamashita, Ichiro, 769 Troutman, W. Brad, 7 Venema, Gerard, 201 Whitnack, Ellen, 350 Yanofsky, Charles, 731 Troy, Frederic A., 845, 854 Verma, Meera, 1381 Wientjes, Frans B., 331 Yasbin, Ronald E., 1283 Truchet, Georges, 1200 Vicente, Miguel, 487, 950 Wierman, Constance K., 425 Yother, Janet, 704 Trust, Trevor J., 263, 1233, Vimr, Eric R., 845, 854 Wiersma, Koos, 201 Young, Frank E., 461 1332 Vink, Robert, 367 Wilcox, Gary, 717, 831 Tsukagoshi, Norihiro, 1182 Wilkinson, Jennifer, 932 Tsung, Kangla, 578 Waghorne, Carol, 960 Williams, Peter A., 887 Zaghloul, Taha I., 550 Walton, Leslie, 470 Wills, John W., 7 Zeikus, J. G., 1146, 1153, Udaka, Shigezo, 1182 Wang, Ming-der, 1350 Willsky, Gail R., 611 1162 Ugalde, Rodolfo A., 1081 Wang, S.-K., 516 Woese, C. R., 230 Ziegler, S. F., 723 Umbarger, H. E., 207, 217 Wauven, Corinne Vander, 882 Woldringh, Conrad L., 331 Zink, Richard T., 390 SUBJECT INDEX VOLUME 164

Acetate E. coli pho constitutive mutants, 1376 P-lactamase II gene cloning, 223 A. vinelandii diauxic growth a- metallothioenzyme gene, 223 effects on glucose uptake system, 866 B. bretvis spores Acetate assimilation B. stearothermophilus amylase gene, germination, 302 M. barkeri, 618 1182 trypsinlike , 302 Acinetobacter sp. synthesis and secretion, 1182 Bacillus licheniformis hexadecane and hexadecanol metabo- chemotaxis alkaline phosphatase lism P. aeruginosa colloidal gold double labeling, 107 alcohol dehydrogenases, 1017 regulation by nitrogen source, 544 L-asparaginase fatty aldehyde dehydrogenases, 1011 Amino acid sequences nitrogen catabolite repression, 938 regulation P. aeruginosa pilin, 571 penicillinase B. subtilis citB, 155 Aminotransferase synthesis colloidal gold double labeling, 107 carbon source, 155 E. coli hisC, 1317 Bacillus spp. Adenine methylation Amylosaccharide metabolism sporulation-induced sigma factor U29, E. coli, 490 C. thermosulfuricum, 1153 1356 Adenylate cyclase C. thermosulfurogenes, 1153 sporulation-related RNA E. coli cya resistance modifications, 1356 cyclic AMP-cyclic AMP receptor pro- E. coli pRR330 Bacillus stearothermophilus tein, 872 gene amplification, 1359 amylase gene expression in B. brevis, negative regulation, 872 neo-cam, 1359 1182 Aeromonas hydrophila recombination sites, 1359 Lipopolysaccharides, 263 Antibiotic-resistant mutants aconitase regulation, 155 O- antigens, 263 H. influenzae, 525 aprA , 550 Aeromcnnas salmonicida Apurinic, apyrimidinic DNA endonuclease calcium transport systems A-layer cell surface array Thermothrix thiopara, 878 cation antiport, 1294 Congo red agar detection, 1233 Arginine catabolism electrogenic uptake, 1294 effects of salt concentrations, 1233 carbon and nitrogen catabolite repres- and DNA cosegregation, 130 virulence determination, 1233 sion, 882 chemotaxis mutants, 802 surface array virulence protein P. cepacia che , 802 Congo red binding, 1332 succinyl derivatives, 882 chloramphenicol acetyltransferase gene, porphyrin binding, 1332 Asparagine synthetase A transcription 550 Agrobacterium rhizogenes E. coli, 310 citB regulation by glucose and root-inducing plasmid ATP glutamine, 155 transferred DNA, 33 B. amyloliquefaciens intracellular lev- DNA binding and entry , 201 Agrobacterium spp. els, 712 electron microscope autoradiography, unique insertion sequence, 918 ATPase activity 130 Agrobacterium tumefaciens mycoplasmalike plant parasites, 811 macrofibers attachment to plant cells, 102 ATP-dependent protease inversion kinetics, 1136 crown gall disease, 723 E. coli lon, 1124 left-handed-twist state, 1141 Ti plasmid virulence ATP synthesis temperature-induced helix hand inver- regulation, 774 R. capsulata sion, 1136 ros negative regulator, 774 nitrous oxide respiration, 823 temperature-pulse-induced "mem- tumor-inducing plasmids, host range, Autoplaquing ory," 1141 723 N. gonorrhoeae, 461 membrane particles agrocin 84 sensitivity, 723 Azospirillum amazonense chromosomal DNA enrichment, 192 virulence nitrogenase penicillin-binding proteins, 192 2-linked-,3-D-glucan, 102 purification and properties, 1271 membrane vesicles, 1294 Alanine-valine transaminase relationship to A. brasilense, 1271 phage SPO2-mediated plasmid transduc- E. coli relationship to A. vinelandii, 1271 tion, 1283 inhibition by P-chloro-L-alanine, 1350 Azotobacter v,inelandii transformation, 201 A-Layer cell surface protein array diauxic growth translational coupling, 550 A. salmonicida acetate-glucose carbon source, 866 Bacillus subtilis- gene fu- Congo red agar detection, 1233 nitrogen fixation, 866 sion effects of salt concentration, 1233 substrate uptake, 866 translational coupling, 550 virulence determination, 1233 Bacterial cell cycle interdependence Alcaligenes eutrophus Bacilluis alcalophilus P. mirabilis, 741 phosphoribulokinase genes quinone identification, 911 chromosomal locations, 954 Bacillus amyloliquefaciens Bacterioopsin expression plasmid locations, 954 o-Amylase export H. halobium revertant, 414 -p-hydroxybutyrate accumulation, ATP intracellular levels, 712 Bacteriophage 749 proton motive force requirement, 712 conversion of lipase, 288 Alcohol dehydrogenases secretion into extracellular medium, E. coli OmpA Acinetobacter sp. metabolism, 1017 712 phage receptor area, 539 Aldolase phage SPO2-mediated transduction, phage-resistant ompA mutants, 539 E. coli 1283 E. coli ung mutations deficient mutants, 845 Bacillus brevis attachment to F-like plasmid pilin, sialic acid catabolism, 845 B. stearothermophilus amylase gene, 1238 sialic acid metabolism, 854 1182 X pKanr, 689 Alginate biosynthesis thermophilic a-amylase synthesis and X pung+, 689 P. aeruginosa gene clustering, 516 secretion, 1182 Alkaline phosphatase production effects of E. coli htpR , 960 iv VOL. 164, 1985 SUBJECT INDEX v

-dependent expression sys- B. cereus, 223 Chloramphenicol resistance tems Bradyrhizobium japonicum P. mirabilis cat expression, 114, 123 construction and regulation, 1366 nodulation-encoding DNA region, 1301 Chondroitin temperature-sensitive c1857 repressor, Brevibacterium lactofermentum B. thetaiotaomicron 1366 lysine pathway genes gene expression in E. coli, 510 S. aureus L form cells, 397 cloning in E. coli, 379 characterization, 510 S. aureus lipase activity, 288 Broad-host-range plasmid RK2, 446 Chromatium spp. SP24 Brochothrix thermosphacta sulfur accumulation, 749 integration into streptococci, 600 glucose phosphotransferase regulation, cis-acting cat-86 regulatory regions, 6% lysogen formation, 600 367 Citrate regulation SPO2-mediated plasmid transduction glucose transport, 367 M. racemosus polypeptide expression, B. amyloliquefaciens, 1283 membrane energization, 367 1049 B. subtilis, 1283 Citrate utilization plasmid T4 dam, 932 Calcium response E. coli cit nucleotide sequence, 977 T5 promoters Y. pestis Citrate utilization system capped mRNA production, 70 Lcrc phenotype, 704 DNA sequence in E. coli, 983 E. coli RNA polymerase, 70 pCD1 requirements, 704 Clindamycin resistance determinant structural analysis, 70 Calcium transport systems B. fragilis, 294 Bacterium W3A1 B. subtilis Clostridium thermohydrosulfuricum expression cation antiport, 1294 amylosaccharide metabolism carbon source regulation, 941 electrogenic uptake, 1294 comparison with C. slime production Campylobacter fetus thermosulfurogenes, 1153 carbon source regulation, 941 cytochromes, 401 catabolite repression-resistant amylase Bacteroides buccae NADPH oxidase activity, 401 synthesis mutants, 1146 surface structure homogeneity oxidation-reduction systems, 401 glucoamylase synthesis, 1146 B. capillus, 1278 Campylobacter species synthesis, 1146 B. pentosaceus, 1278 morphological forms and viability, 338 Clostridium thermosulfurogenes Bacteroides capillus Candida albicans amylosaccharide metabolism surface structure homogeneity mitochondrial DNA, 7 comparison with C. B. buccae, 1278 Capsular polysaccharide thermohydrosulfuricum, 1153 B. pentosaceus, 1278 E. coli lon mutants, 1124 13-amylase production Bacteroides-flavobacteria relatedness, 230 methylation hyperproductive mutants, 1162 Bacteraides fragilis M. xanthus, 495 regulation and genetic enhancement, clindamycin resistance determinant, 294 Carotenoids 1162 cloning systems, 294 Synechocystis sp. outer membrane, 384 Coenzyme A polyethylene glycol-facilitated transfor- Carotovoricin induction E. coli regulation, 136 mation, 466 E. carotovora recA, 390 Colicin El Tn4400 Cations COOH-terminal , 237 compound transposon, 1248 P. aeruginosa lipopolysaccharide af- E. coli spontaneous induction, 1362 E. coli replicon fusion and transposi- finity, 1256 imm, 237 tion, 1248 cat-86 mRNA immunity protein-reactive domain, 237 insertion sequence components, 1248 chloramphenicol-induced translation, Coliphage 186 transformation with plasmid DNA, 466 696 E. coli AB1157 infection Bacteroides pentosaceus cis-acting regulatory regions, 696 source phenotypic variations, 1381 surface structure homogeneity ribosome-binding sites, 6% UV irradiation effects, 1381 B. buccae, 1278 Caulobacter halobacteroides Colloidal gold labeling B. capillus, 1278 glycolipid levels, 684 B. licheniformis Bacteroides spp. phospholipid levels, 684 alkaline phosphatase, 107 crystalline surface layer structures, 1278 cDNA library penicillinase, 107 homogeneity of biochemical characteris- E. coli IS186, 957 Congo red agar tics Cell cycle interdependence A. salmonicida A-layer detection, 1233 B. buccae, 1278 P. mirabilis, 741 Congo red binding B. capillus, 1278 Cell elongation A. salmonicida, 1332 B. pentosaceus, 1278 E. coli wee dnaA mutant, 487 Corrins Bacteroides thetaiotaomicron Cell surfaces M. bryantii methylreductase system, chondroitin lyase gene S. sanguis 165 characterization, 510 adherence molecules, 255 Crown gall disease expression in E. coli, 510 hydrophilic variants, 255 A. tumefaciens P-Amylase synthesis Cell wall and DNA cosegregation agrocin 84 sensitivity, 723 C. thermrosulfurogenes B. subtilis, 130 tumor-inducing plasmids, host range, hyperproductive mutants, 1162 electron microscope autoradiography, 723 regulation and genetic enhancement, 130 Cyanelles 1162 Chemotaxis C. paradoxa Betaine E. coli, 1057 cyanobacterial endosymbiont source, S. typhimurium H. halobium mutants, 282 659 osmoprotectant function, 1218, 1224 P. aeruginosa photosynthetic protein genes, 659 proP, 1218 regulation by nitrogen source, 544 Cyanobacteria proU, 1224 Chemotaxis mutants N. commune uptake, 1218, 1224 B. subtilis che, 802 matric water stress, 1025 3-Galactosidase Chlamydia trachomatis protein synthesis and proteolysis, E. coli guaC regulation, 1288 surface projections, 344 1025 E. coli lac up-promoter mutants, 1353 Chloramphenicol-induced cat-86 mRNA, Cyanophora paradoxa 1-Lactamase II 6% cyanelle genome map, 659 vi SUBJECT INDEX J. BACTERIOL

protein localization, 659 expression in S. typhimurium, 717 dinucleoside tetraphosphate reduction, Cyclic AMP nucleotide sequence, 717 63 E. coli adenylate cyclase gene expres- chromosomal mapping DNA-adenine methyltransferase (dam) sion, 872 IncP plasmid R68::Mu, 1110 evolutionary origin, 932 synthesis plant soft-rot disease relationship to phage T4 dam, 932 S. typhimurium enzyme IlGlIc regula- pectic enzymes, 831 dnaA expression tion, 477 tissue maceration, 831 chromosome replication, 918 V. fisheri luminescence control, 45 virulence determi- dnaA repression Cytochrome c nant, 831 thermosensitivity, 918 P. denitrificans mutants, 1064 prophage Mu DNA-protein interactions, 490 Cytochrome c' marker mobilization frequency, 1110 DNA replication R. capsulata electron transport, 823 Erwinia carotovora subsp. carotovora cell division coupling, 950 Cytochromes nonpathogenic mutant FtsA protein mediation, 950 C. fetus, 401 sensitivity to galactose, 473 E. carotovora ara expression, 717 UDP glucose-pyrophosphorylase mu- exonuclease-deficient strains, 1362 D-Alanyl lipoteichoic acid tation, 473 frdABCD, 1100 S. aureus, 1211 recA fumarate reductase genes Desulfovibrio gigas carotovoricin induction, 390 coordinate regulation, 1100 heme synthesis, 316 lyase induction, 390 transcription, 1100 protoporphyrinogen oxidation, 316 Erwinia chrysanthemi -y-glutamyl kinase Detergent resistance pectate lyase isozyme genes, 51 osmotic tolerance, 1088 S. aureus pel mutagenesis, 51 proBA operon mutations, 1088 Triton X-100 interaction, 1337 soft-rot pathogenesis, 51 overproduction, 1088 Diadenosine tetraphosphatase Erwinia herbicola gInALG operon regulation, 816 E. coli, 63 ice nucleation genes, 359 glnALG upstream promoter mutation, Diauxic growth Erythromycin A production 1032 A. vinelandii, 866 genetic analysis, 425 glnB Pi, product Dictyostelium discoideum S. erythreus, 425 glutamine synthetase synthesis, 816 cell differentiation Escherichia coli histidase synthesis, 816 effects of folate, 143 adenine methylation, 490 glutamine synthetase-constitutive muta- phosphodiesterase induction adenylate cyclase (cya) gene expression tion, 1032 folate, 143 cya-lac protein and operon fusion glycogen accumulation, 749 folate analogs, 143 plasmids, 872 guaC regulation Dihydrofolate reductase cyclic AMP-cyclic AMP receptor pro- ,-galactosidase, 1288 E. coli, 470 tein, 872 GMP reductase, 1288 Divalent cations negative regulation, 872 lac fusion technique, 1288 K. pneumoniae glycerol dehydrogenase, alanine-valine transaminase hemolysin complexes, 1071 479 0-chloro-L-alanine inhibition, 1350 hisC cloning, structure, and expression, DNA-adenine methyltransferase alkaline phosphatase production, 1376 1317 E. coli dam alpha-hemolysin complex structure hisC gene product, 1317 evolutionary origin, 932 hlyA protein, 1071 hlyA protein, 1071 relationship to phage T4 dam, 932 lipopolysaccharide component, 1071 host primase plasmid requirement, 484 DNA killer plasmids release mechanisms, 1071 htpR (rpoH) temperature-sensitive mu- glycoprotein killer toxin, 1373 toxicity, 1071 tation K. kluyveromyces aminotransferase synthesis, 1317 cell growth, 960 transfer to C. pseudotropicalis, 1373 antibiotic resistance gene amplification heat shock polypeptide synthesis, 960 transfer to K. fragilis, 1373 neo-cam, 1359 phage A development, 960 DNA methylation in spiroplasmas pRR330, 1359 hydrogenase isoenzymes procaryotic and eucaryotic traits, 19 recombination sites, 1359 differential expression, 1324 DNA-protein interactions asnA-asnC regulation, 310 unidentified third isoenzyme, 1324 E. coli, 490 asparagine synthetase A gene, 310 hyp gene product, 321 DNase activity B. fragilis Tn4400 functions, 1248 ice nucleation gene expression, 359 mycoplasmalike plant parasites, 811 ,-galactosidase levels, 1353 ilvGEDA operon, 207 DNA sequence binding proteins, 1057 IS186 isolation P. mirabilis cat gene, 123 B. thetaiotaomicron chondroitin lyase cDNA libraries, 957 DNA supercoiling expression, 510 K. pneumoniae pulA expression, 633, S. typhimurium top mutants, 947 chemotaxis, 1057 639 chloramphenicol-dependent lysis lac up-promoter mutants, 1353 Electron microscope autoradiography relA+ peptidoglycan synthesis, 861 lacY gene product B. subtilis cell wall and DNA cosegrega- chromosome replication R. meliloti proton motive force, 929 tion, 130 dnaA induction, 918 lon Endoribonuclease cit nucleotide sequence, 977 ATP-dependent protease, 1124 S. cerevisiae, 57 citrate utilization plasmid, 977, 983 capsular polysaccharide mutants, Enteric ilv leader regions, 207 coenzyme A regulation, 136 1124 Enzyme IIIcGc colicin El synthesis, 1362 insertional mutagenesis, 1124 S. typhimurium cyclic AMP synthesis, consensus promoter sequences, 1353 UV sensitivity mutants, 1124 477 deoxyribonucleotide levels, 1194 A promoter-dependent expression sys- E protein dfp tems, 1366 incB and incC, 1262 effects on DNA synthesis, 994 maltose-binding protein plasmid F replication control, 1262 flavoprotein product, 994 in vivo and in vitro synthesis, 665 Erwinia caotovora diadenosine tetraphosphatase gene, 63 lacUV5 promoter-operator regulation, araA and araC dihydrofolate reductase-deficient mu- 665 expression in E. coli, 717 tant, 470 malE, 665 VOL. 164, 1985 SUBJECT INDEX vii

maltose transport, 1057 multiple genetic control, 845 E. coli frdABCD transcriptional regula- membrane particles nan, 845 tion, 1100 chromosomal DNA enrichment, 192 sialic acid metabolism penicillin-binding proteins, 192 interconversions, 854 y-Glutamyl kinase meso-diaminopimelic acid incorporation N-acylneuraminate pyruvate-lyase, E. coli kinetics, 331 854 osmotolerance, 1088 methylase-sensitive phenotype analysis SOS protein proBA operon mutations, 1088 P. vulgaris restriction-modification recA +-lexA -dependent induction, proline overproduction, 1088 system, 501 653 Glucoamylase mitomycin C resistance, 836 recN product, 653 C. thermohydrosulfuricum nar operon regulatory regions, 25 strain AB1157 cultivars catabolite repression-resistant mu- new DNA repair pathway, 1309 coliphage 186 infection, 1381 tants, 1146 nitrate reductase production, 25 source phenotypic variations, 1381 Glucose phosphotransferase system ompB protein products "TER" pathway B. thermosphacta regulation, 367 EnvZ proteins, 578 SOS-independent pathway, 950 Glutamine synthetase OmpR proteins, 578, 585 TOL plasmids T. ferrooxidans gInA OmpF and OmpC biosynthesis, 578, 585 meta pathway enzyme genes, 887 cloning in E. coli, 1386 ompF-ompC chimeric genes trmA Glutamine synthetase-constitutive muta- construction, 797 monocistronic operon, 1117 tion translational products, 797 organization and transcription, 1117 E. coli glnALG upstream promoter, osmoregulation tRNA (-5)methyltransferase, 1032 potassium transport, 434 1117 Glycine max proline transport, 434 tryptophanase operon expression proton motive force, 1383 osmoresponsive genes, 434 p factor mutants, 731 Glycolipids outer membrane protein OmpA tna regulation, 731 C. halobacteroides, 684 phage receptor area, 539 transcription antitermination control, GMP reductase phage-resistant ompA mutants, 539 731 E. coli guaC regulation, 1288 oxygen-induced DNA damage, 1309 type 1 piliation, 321 Gram-negative bacteria, 446 panF mutants, 136 ung mutation selection procedures insertion sequence elements, entrap- pantothenate permease mutants, 136 phage A pKanr, 689 ment, 918 penicillin-binding protein 3 site, 456 phage A pung+, 689 Gyrase peptidoglycan biosynthesis uracil-DNA mutants, 689 H. influenzae chloramphenicol-dependent lysis, 861 V. fisheri luminescence control activity, 535 inhibitors and autolysis, 861 catabolite repression, 45 B subunit gene copies, 535 peptidoglycan synthesis, 331 cyclic AMP receptor protein, 45 mutations, 525 pho constitutive mutants lux gene regulation, 45 alkaline phosphatase excretion, 1376 wee dnaA mutant cell length, 487 Haemophilus influenzae plasmid transformation, 1376 Escherichia coli-Bacillus subtilis gene fu- antibiotic-resistant mutants, 525 phosphoenolpyruvate carboxylase-def- sion gyrase icient mutant, 646 translational coupling, 550 activity, 535 phs mutation Ethanol B subunit gene copies, 535 araBAD transcription defect, 972 Z. mobilis mutations, 525 arabinose metabolism, 904 decreased fermentation, 173 Halobacterium halobium L-glutamate metabolism, 904 increased membrane leakage, 173 bacterioopsin gene expression in a re- melibiose metabolism, 904 Euglena gracilis vertant, 414 Na+/H+ antiport system, 904 bop expression restoration, 414 pleiotropic lesions in metabolism, 972 catalytic properties, 762 photosensory transduction, 282 rpoA allele, 972 immunological properties, 762 phototaxis-deficient mutants, 282 sulfur metabolism, 904 purification, 762 sensory rhodopsin, 282 pilA control, 321 Heat shock polypeptide synthesis plasmid pSC101 maintenance, 484 Fatty aldehyde dehydrogenases E. coli htpR mutation, 960 plumbagin-induced mutagenicity, 1309 Acinetobacter sp. metabolism, 1011 Heme synthesis promoter mutants, 1353 Fermentation D. gigas, 316 protein Bi missense mutations, 1194 Z. mobilis Hemolysin complexes proteolytic enzymes, 1124 ethanol inhibition, 173 E. coli recBC mutant phenotype suppression, increased membrane leakage, 173 hlyA protein, 1071 836 Fibrinogen lipopolysaccharide component, 1071 recBC sbcB strains, 836 group A streptococci release mechanisms, 1071 Rep protein promoter and N terminus, binding to M protein, 350 toxicity, 1071 1004 Flagella Hook-associated proteins ribonucleotide reductase mutants, 1194 S. typhimurium, 1370 S. typhimurium RNA polymerase Flavobacteria-bacteroides relatedness, 230 exctetion, 1370 phage T5 promoter recognition, 70 Flavoprotein synthesis, 1370 rpoA mutation E. coli dfp gene product, 994 Hydrogenase effects of phs mutation, 904 Folate P. denitrificans mutants, 1064 ruv gene product, 276 D. discoideum R. japonicum ruv mutants cell differentiation, 143 subunit composition, 187 resistance to mitomycin C, 276 phosphodiesterase induction, 143 uptake activity, 187 resistance to UV light, 276 Fumarase Hydrogenase isoenzymes sbcC mutations, 836 E. gracilis var. bacillaris E. coli S. citri spiralin gene expression, 1094 immunological properties, 762 differential expression, 1324 sialic acid catabolism purification, 762 unidentified third isoenzymes, 1324 aldolase-negative mutants, 845 Fumarate reductase Hydrophobicity viii SUBJECT INDEX J. BACTERIOL

S. sanguis, 255 O-polysaccharide morphology, 263 methanol oxidation P. aeruginosa energized membrane, 95 Ice nucleation genes cation binding, 1256 sodium ions, 95 E. herbicola, 359 polyamine affinity, 1256 monofluoroacetate resistance, 618 expression in E. coli, 359 polycationic-antibiotic affinity, 1256 surface antigens, 1 P. syringae, 359 Synechocystis sp. outer membrane, 384 Methanosarcinaceae ilvGEDA operons Lysine pathway envelope structure, 1 comparison in enteric organisms, 207 B. lactofermentum genes, 379 surface antigens, 1 S. marcescens E. coli expression, 379 Methylation leucine regulation, 217 M. xanthus, 495 Immunity protein MAIS organisms Mitochondrial DNA colicin E1 reactive domain, 237 mycobactin 'structures, 896 C. albicans, 7 COOH-terminal peptide, 237 Maltose-binding protein Mitomycin C Inorganic phosphate transport E. coli, 1057 E. coli resistant mutants, 836 S. cerevisiae PHO2, 964 in vivo and in vitro synthesis, 665 Mitomycin C resistance Insertion elements IacUV5 promoter-operator control, E. coli ruv mutants, 276 E. coli IS186 isolation, 957 665 Mollicutes Insertion sequence malE, 665 S. citri spiralin gene RNA species, 556 Maltose fermentation expression in E. coli, 1094 Intracytoplasmic inclusions S. carlsbergensis MAL6, 605 Molybdenum A. eutrophus poly-i-hydroxybutyrate Maltose transport P. denitrificans mutants, 1064 accumulation, 749 E. coli, 1057 Molybdenum cofactor biosynthesis Chromatium spp. sulfur accumulation, Marine bacterium K. pneumoniae, 1081 749 C. halobacteroides lipid levels, 684 Monoclonal antibodies E. coli glycogen accumulation, 749 Matric water stress M. barkeri surface antigen s, 1 effects on volume and N. commune, 1025 Mucor racfmosus density, mathematical model, 749 Membrane biogenesis conditional developmental mutant, 1049 physical composition, 749 R. sphaeroides phospholipid transfer, glycolytic enzyme expression, 1049 Inversion kinetics 181 morphogenesis, 1039, 1049 B. subtilis macrofibers, 1136 Membrane particles mycelial growth, 1039, 1049 Iron-molybdenum cofactor biosynthesis B. subtilis, 192 oxidative metabolism, 1049 K. pneumoniae, 1081 E. coli, 192 polypeptide expression, 1039, 1049 IS10 Membrane protein Multicopy single-stranded DNA pOUT promoter RNA product, 556 S. citri spiralin bacterial species distribution, 914 expression in E. coli, 1094 myxobacteria, 914 Klebsiella pneumoniae Menaquinone Mycelial growth divalent cations, 479 B. alcalophilus, 911 M. racemosus, 1039, 1049 glycerol dehydrogenase inactivation, meso-Diaminopimelic acid AMycobacterium spp. 479 E. coli incorporation and uptake kinet- mycobactin structures, 896 iron-molybdenum cofactor biosynthesis ics, 331 Mycobactins sulfur source effects, 1Q81 peptidoglycan synthesis, 331 MAIS complex, 896 maltose-inducible protein, 633, 639 Metallothioenzyme Mycobacterium spp. malT promoter, 639 B. cereus ,3-lactamase II, 223 structural analyses, 896 molybdenum cofactor biosynthesis Methane production Mycoplasmalike organisms sulfur source effects, 1081 M. barkeri, 618 aster yellows-infected plant hosts pulA, 633, 639 Methanobacterium bryantii enzymatic activities, 811 pulA promoter, 639 methylreductase system Myxobacteria pullulanase gene, 633, 639 corrin activation, 165 multicopy single-stranded DNA, 914 sulfur source effects methanogenesis, 165 Myxococcus xanthus iron-molybdenum cofactor biosynthe- Methanogenesis fruiting body formation, 270 sis, 1081 M. bryantii methylreductase system methylation of macromolecules, 495 molybdenum cofactor biosynthesis, corrin activation, 165 one-step cloning vector, 270 1081 Methanol dehydrogenase Kluyveromyces lactis P. denitrificans mutants, 1064 NADH oxidase activity DNA killer plasmids Methanol metabolism mycoplasmalike plant parasites, 811 glycoprotein killer toxin, 1373 P. denitrificans NADPH activity transfer to C. pseudotropicalis, 1373 cytochrome c mutants, 1064 C. fetus, 401 transfer to K. fragilis, 1373 formaldehyde metabolism mutants, NADPH oxidase activity 1064 mycoplasmalike plant parasites, 811 lac fusion technique hydrogenase mutants, 1064 nar operon regulatory regions E. coli guaC regulation, 1288 methanol dehydrogenase mutants, E. coli, 25 IacUV5-malE plasmid construction, 665 1064 Neisseria gonorrhoeae L-Asparaginase molybdenum cofactor mutants, 1064 autoplaquing, 461 B. licheniformis nitrogen catabolite re- one-carbon metabolism mutants, 1064 Nitrate reductase derepression pression, 938 Methanol oxidation R. japonicum Lipase activity energized membrane, 95 effect of cyclic GMP, 757 S. aureus M. barkeri, 95 Nitrate reductase production bacteriophage conversion, 288 sodium ions, 95 E. coli, 25 Lipids Methanosarcina barkeri nar regulatory regions, 25 C. halobacteroides, 684 acetate assimilation, 618 Nitrogenase Synechocystis sp. outer membrane, 384 carbon assimilation, 618 A. amazonense Lipopolysaccharides envelope structure, 1 purification and properties, 1271 A. hydrophila methane production, 618 relationship to A. brasilense, 1271 VOL. 164, 1985 SUBJECT INDEX ix

relationship to A. vinelandii, 1271 Penicillin-binding proteins NRl-derived pRR330 A. brasilense, 1271 B. subtilis membrane particles, 192 drug resistance gene amplification, A. vinelandii, 1271 E. coli membrane particles, 192 1359 Nitrogenase synthesis Penicillium chrysogenum E. coli neo-cam, 1359 R. japonicum sulfate-activating enzymes, 674 recombination sites, 1359 effects of cyclic GMP, 757 Penicillium duponti pCD1 and Y. pestis low-calcium Nitrogen catabolite sulfate-activating enzymes, 674 response, 704 B. licheniformis Peptidoglycan biosynthesis pDL316 L-asparaginase repression, 938 E. coli, 331 homology with S. faecalis pJH1, 626 Nitrogen fixation E. coli autolysis, 861 phage SPO2-mediated transduction A. vinelandii, 866 Phosphate accumulation B. amyloliquefaciens, 1283 R. melilotiji&F, 245 S. cerevisiae, 611 B. subtilis, 1283 Nitrous oxide reductase Phosphodiesterase induction pJH1 pAMaj,%1-like replicon, 626 R. capsulata, 823 D. discoideum, 143 plasmid DNA and B. fragilis transfor- Nitrous oxide reduction Phosphoenolpyruvate carboxylase mation, 466 Rhodospirillaceae, 823 E. coli mutant, 646 pOUT promoter RNA product, 556 N-Methyl-N'-nitro-N-nitrosoguanidine immunological purification technique, P. putida TOL plasmids S. fradiae 646 meta pathway enzyme genes, 887 adaptive response, 944 Phosphoenolpyruvate:sugar phosphotrans- pSC101 maintenance in E. coli, 484 chloramphenicol mutagenic enhance- ferase system pSym2011 ment, 944 S. typhimurium R. meliloti nodulation, 1200 Nodulation enzyme 111Ic regulation of cyclic R. meliloti root hair curling, 1200 B. japonicum, 1301 AMP, 477 R68: :Mu regulation by plant hosts, 591 Phospholipids E. carotovora IncP plasmid, 1110 R. fredii, 1301 C. halobacteroides, 684 root-inducing A. rhizogenes plasmid, 33 R. meliloti, 410 Phospholipid transfer activity root tumor formation, 33 host specificity-controlling pSym2011 R. sphaeroides, 181 SEP1 region, 1200 Phosphoribulokinase genes S. erythreus sex factor, 969 nod alfalfa nodulation region, 1200 A. eutrophus, 954 TOL plasmids R. meliloti nod, 245 Photosynthesis meta pathway enzyme genes, 887 R. meliloti nodA, 591 C. paradoxa cyanelle genome, 659 Pleiotropic lesions Nostoc commune Photosynthetic membrane biogenesis E. coli phs mutation, 972 matric water stress, 1025 R. sphaeroides, 181 Plumbagin toxicity and mutagenicity protein synthesis and proteolysis, 1025 Phototaxis E. coli, damage and repair H. halobium mutants, 282 compared with H202, 1309 S. aureus sensory rhodopsin, 282 compared with SOS agents, 1309 expression in E. coli, 925 Pilin p-nitrophenyl phosphatase activity Nucleotide sequences F-like plasmids, 1238 mycoplasmalike plant parasites, 811 P. aeruginosa pilin, 571 P. aeruginosa Polygalacturonase amino acid sequences, 571 E. carotovora virulence determinant, ompB product nucleotide sequences, 571 831 E. coli synthesis regulation, 578, 585 phage attachment, 1238 Polypeptide expression One-step cloning vector Pilus production M. racemosus, 1039, 1049 M. xanthus genes, 270 E. coli Polypeptide methylation Osmoregulation hyp gene product, 321 M. xanthus, 495 E. coli osrA and kdp, 434 pilA transcription, 321 Porphyrin binding K+ transport, 434 Plant parasites A. salmonicida, 1332 L-proline, 434 mycoplasmalike organisms Proline overproduction Osmotolerance aster yellows-infected hosts, 811 E. coli y-glutamyl kinase, 1088 E. coli y-glutamyl kinase, 1088 enzymatic activities, 811 Promoter mutants Outer membrane porin Plant soft-rot disease E. coli E. coli synthesis regulation, 578, 585 E. carotovora polygalacturonase, 831 3-galactosidase levels, 1353 Outer membrane protein Plasmids consensus promoter sequences, 1353 E. coli OmpA A. rhizogenes root-inducing plasmid lac up-promoter mutants, 1353 phage receptor area, 539 transferred DNA, 33 Promoter mutation phage-resistant ompA mutants, 539 A. tumefaciens Ti plasmid E. coli gln, 1032 Outer membrane proteins ros virulence regulation, 774 Promoters E. coli opmF-ompC chimeric genes, 797 A. tumefaciens tumor-inducing plas- phage T5, 70 mids, 723 Prophage Mu panF mutants broad-host-range plasmid RK2, 446 E. carotovora marker mobilization fre- E. coli, 136 E. coli citrate utilization plasmid, 977, quency, 1110 Pantothenate permease mutants 983 Protease coenzyme A regulation, 136 E. coli ColEl B. subtilis macrofiber orientation, 1141 E. coli panF, 136 colicin El synthesis, 1362 Protein Bi Paracoccus denitrificans E. coli cya-lac protein and operon fu- E. coli missense mutations, 1194 methanol oxidation mutants, 1064 sion, 87 Protein export Pectate lyase isozyme E. coli TOL plasmids B. amyloliquefaciens E. chrysanthemi pel genes, 51 meta pathway enzyme genes, 887 proton motive force requirement, 712 soft-rot pathogenesis, 51 F-like pilin genes, 1238 Protein methylation Pectin lyase induction F replication control system M. xanthus, 495 E. carotovora recA, 390 effect of incB and incC, 1262 Protein synthesis and proteolysis Penicillin-binding protein 3 E protein functions, 1262 N. commune, 1025 E. coli, 456 K. lactis DNA killer plasmids, 1373 Proteolytic enzymes x SUBJECT INDEX J. BACTERIOL

E. coli Lon, 1124 Rhizobium fredii A. rhizogenes root-inducing plasmid Proteus mirabilis nodulation-defective mutant, 1301 transferred DNA, 33 cat expression, 114, 123 Rhizobium japonicum cat nucleotide sequence, 123 ca,a-trehalose accumulation, 78 Saccharomyces carlsbergensis cell cycle interdependence bacteroids MAL6 , 605 biosynthetic cycle, 741 trehalose synthesis, 78 maltose heat lability, 605 interdivision cycle, 741 carbohydrate accumulation, 78 Sacc haromyces cerevisiae cell wall extension, 741 cyclic GMP effects endoribonuclease chloramphenicol resistance, 114, 123 growth inhibition, 757 cleavage products, 57 DNA replication, 741 nitrogenase synthesis, 757 -specific cleavage, 57 septum formation, 741 respiratory nitrate reductase derepres- RNA cleavage, 57 type I (Tn9) cat variant, 123 sion, 757 inorganic phosphate transport systems, Proteus vulgaris hydrogenase subunit composition, 187 964 E. coli methylase-sensitive phenotype, hydrogenase uptake activity, 187 PH02 gene function, 964 501 proton motive force, 1383 phosphate accumulation, 611 restriction-modification system, 501 trehalose accumulation, 78 vanadate-resistant mutants, 611 Proton motive force Rhizobium meliloti vanadium metabolism, 611 B. amyloliquefaciens a-amylase export, fixF characterization, 245 Saccharomyces diastaticus 712 N2 fixation, 245 STAI expression G. max bacteroides, 1383 nod gene products, 591 glucoamylase production, 769 R. japonicum washed cells, 1383 nodulation, 245 MATa2 mutation, 769 R. meliloti, 929 host specificity-controlling pSym2011 mating-type control, 769 Protoporphyrinogen oxidation region, 1200 Salmonella typhimurium D. gigas, 316 nod alfalfa nodulation region, 1200 crp strains Pseudobactin nodulation competitiveness phosphoenolpyruvate:sugar phospho- P. putida siderophore biosynthesis, 563 cryptic plasmid pTA2, 410 , 477 Pseudomonas aeruginosa rifampin resistance mutation, 410 E. carotovora ara expression, 717 alginate gene clustering, 516 nodulation genes, 591 enzyme IlIGlIc regulation of cyclic AMP, alginic acid biosynthesis, 516 proton motive force, 929 477 cation binding to lipopolysaccharides, root hair curling flagella, 1370 1256 host specificity-controlling pSym2011 hook-associated proteins chemotaxis regulation by nitrogen region, 1200 excretion, 1370 source, 544 Rhodopseudomonas capsulata synthesis, 1370 pilin amino acid and nucleotide ATP synthesis, 823 proP- and proU-encoded betaine trans- sequences, 571 electron transport port, 1218, 1224 polyamine affinity for LPS, 1256 cytochrome c', 823 proP osmoregulation, 1218 polycationic-antibiotic affinity for LPS, nitrous oxide reductase, 823 proU osmoregulation, 1224 1256 Rhodopseudomonas sphaeroides top mutants Pseudomonas cepacia mutant complementation, 147 DNA supercoiling, 947 arginine catabolism phospholipid transfer activity leu-500 promoter mutation suppres- succinyl derivatives, 882 intracellular localization, 181 sion, 947 Pseudomonas putida membrane biogenesis, 181 Scanning electron microscope study scanning electron microscope analysis ribulose 1,5-bisphosphate P. putida cell morphologies, 1171 carboxylase/oxygenase cell morphologies, 1171 colony microstructure, 1171 form I, 1188 colony microstructure, 1171 extracellular materials, 1171 form II, 1188 extracellular materials, 1171 multicellular arrangements, 1171 ribulose bisphosphate multicellular arrangements, 1171 siderophore biosynthesis genes, 563 carboxylase-oxygenase regulatory Sensory rhodopsin TnS mutagenesis, 563 mutant, 147 H. halobium phototaxis, 282 TOL plasmids Rhodospirillaceae Serratia marcescens meta pathway enzyme genes, 887 nitrous oxide reduction, 823 ilvGEDA operon regulation, 217 Pseudomonas syringae Ribonucleotide reductase Sex factor ice nucleation genes, 359 E. coli mutants, 1194 S. erythreus plasmid SEP1, 969 Pseudomonas syringae pv. syringae Ribulose bisphosphate Sialic acid syringotoxin production mutants, 14 carboxylase-oxygenase E. coli inducible catabolic system, 845 Tn5 insertion mutants, 14 R. sphaeroides regulatory mutant, 147 E. coli metabolism, 854 Pullulanase Ribulose 1,5-bisphosphate Siderophore biosynthesis C. thermohydrosulfuricum carboxylase/oxygenase P. putida, 563 catabolite repression-resistant mu- R. sphaeroides forms I and II Slime production tants, 1146 subunit structural differences, 1188 bacterium W3A1 K. pneumoniae, 633, 639 Rifampin resistance regulation by carbon source, 941 Pyrimidine-specific cleavage R. meliloti, 410 SOS protein S. cerevisiae endoribonuclease, 57 RNA polymerase E. coli E. coli recA +-lexA +-dependent induction, Quinone phage T5 promoter recognition, 70 653 B. alcalophilus, 911 RNase activity recN product, 653 mycoplasmalike plant parasites, 811 Spiroplasma citri rep regulatory region Root hair curling spiralin gene E. coli, 1004 R. meliloti expression in E. coli, 1094 Restriction-modification system host specificity-controlling pSym2011 Spiroplasmas E. coli methylase-sensitive phenotype, region, 1200 DNA methylation patterns, 19 501 nod alfalfa nodulation region, 1200 procaryotic and eucaryotic traits, 19 P. vulgaris, 501 Root tumor induction Spores VOL. 164, 1985 SUBJECT INDEX xi

B. cereus Streptomyces fradiae plasmid DNA, 466 germination, 302 N-methyl-N'-nitro-N-nitrosoguanidine polyethylene glycol facilitation, 466 trypsinlike enzymes, 302 adaptive response, 944 B. subtilis, 201 Sporulation chloramphenicol mutagenic enhance- Transposon mutagenesis Bacillus spp. ment, 944 P. syringae syringotoxin mutants, 14 RNA polymerase modifications, 1356 Streptomyces griseus Transposon Tn917 nucleotide sequence sigma factor o29 induction, 1356 str cloning, 85 erm determinant, 782 STAI streptomycin biosynthesis, 85 S. faecalis, 782 S. diastaticus glucoamylase production streptomycin resistance, 85 Trehalose accumulation MATa2 mutation, 769 Streptomycin biosynthesis R. japonicum, 78 mating-type control, 769 S. griseus mutants, 85 R. japonicum bacteroids, 78 Staphylococcus aureus Streptomycin resistance Triton X-100 D-alanyl lipoteichoic acid reesterifica- S. griseus mutants, 85 S. aureus tion, 1211 Substrate uptake detergent-resistant mutants, 1337 geh insertional inactivation, 288 A. vinelandii, 866 interaction, 1337 L form cells Sulfate-activating enzymes tRNA (uracil-5)methyltransferase lysogenicity, 397 mesophiles versus thermophiles, 674 E. coli trmA, 1117 phage typability, 397 P. chrysogenum, 674 Trypsinlike enzymes lipase activity P. duponti, 674 B. cereus spores, 302 bacteriophage conversion, 288 Surface projections of chlamydiae, 344 germination, 302 nuclease expression in E. coli Synechocystis sp. Tryptophanase expression export defect, 925 carotenoid-containing outer membrane, E. coli tna regulation, 731 suppression by prlA mutations, 925 384 toluene treatment effects, 1211 outer membrane components UDP glucose-pyrophosphorylase Triton X-100 interaction, 1337 carotenoids, 384 E. carotovora galactase-sensitive mu- Triton X-100-resistant mutants, 1337 lipids, 384 tant, 473 Streptococci lipopolysaccharides, 384 Uracil-DNA glycosylase group A proteins, 384 E. coli mutants, 689 fibrinogen binding to M protein, 350 Syringotoxin T. thiopara, 421 lysogen formation, 600 P. syringae, 14 UV light resistance M protein antiopsonic property, 350 E. coli ruv mutants, 276 M protein expression, 600 "TER" pathway UV sensitivity phage SP24 integration, 600 E. coli FtsA protein E. coli lon mutants, 1124 Streptococcus faecalis cell division-DNA replication cou- erm determinant, 782 pling, 950 Vanadium metabolism macrolide-lincosamide-streptogramin Thermosensitivity S. cerevisiae vanadate-resistant mu- B-resistance transposon E. coli dnaA repression, 918 tants, 611 nucleotide sequence, 782 Thermothrix thiopara Vibrio fischeri R plasmid pJH1 apurinic, apyrimidinic DNA endonucle- luminescence expression in E. coli, 45 pAMalAl-like replicon, 626 ase, 878 luminescence system control pDL316 homology, 626 uracil-DNA glycosylase, 421 catabolite repression, 45 cyclic AMP receptor protein, 45 Tn9J7 nucleotide sequence, 782 Thiobacillus ferrooxidans lux gene regulation, 45 Streptococcus sanguis gInA cloning in E. coli, 1386 Virulence cell surface components glutamine synthetase gene A. tumefaciens, 102 adherence, 255 cloning in E. coli, 1386 aggregation, 255 TnlO transposition Yersinia pestis hydrophobicity, 255 ISJO translation inhibition, 556 Ca2'-blind mutants, 704 Streptomyces erythreus Toluene treatment low-calcium-response-constitutive mu- erythromycin production, 425 S. aureus lipoteichoic acid synthesis, tants, 704 sex factor 1211 plasmid SEP1, 969 Transformation Zymomonas mobilis zygosis-sensitive mutant, 969 B. fragilis ethanol fermentation inhibition, 173 U.S. Postal Service STATEMENT OF OWNERSHIP MANAGEMENT AND CIRCULATION ResuireJ bv 39 U.S.C. 3685/ lA.TITLE OF PUBLICATION 1B. PUBLICATION NO.-- 2. DATE OF FILING Journal Qf Bacteriology 1 October 1985

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Please check: El Enclosed is my Membership Fee, includes ASM News (in U.S. dollars only) ...... $10 EJ Please send me the following ASM journal(s) at Member Price(s): Journals U.S. Non-U.S. Amount Antimicrobial Agents and Chemotherapy $35 $49 $ . - Applied and Environmental Microbiology 35 49 Molecular and Cellular Biology 41 54 Infection and Immunity 41 54 International Journal of Systematic Bacteriology 35 35 Journal of Bacteriology 41 54 Journal of Clinical Microbiology 35 49 Journal of Virology 41 54 Microbiological Reviews 18 29 Total Joumal Fees $ S Add your $10 Membership Fee +10 Total $ S

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0 VISA # 0 MASTERCARD # EXPIRATION DATE TODAYS DATE SIGNATURE Minimum Charge $15.00 1986 APPLICATION FOR FULL MEMBERSHIP IN THE AMERICAN SOCIETY FOR MICROBIOLOGY 1913 1 Street, NW * Washington, DC 20006 * (202) 833-9680 COMPLETE ALL INFORMATION REQUESTED AND RETURN FORM WITH REMITTANCE IN U.S. FUNDS Eligibility ASM welcomes to fullmembership anyone who is interested In its objectives and has a minimum of a bacheloes de- gree orequivalent in microbiologyora related field. Initiation Membershipsare initiated and renewed inJanuaryeachyear. Unlessthereare directionstothecontrary, membership nomirlations received prior to November 1 are credited to the current year, and back issues of the selected publications for the current year are furnished, if available. Nominations received after November 1 will become effective the following January. NAME FIRST INITIAL LAST ADDRESS CITY STATE/PROVINCE ZIP/POSTAL CODE COUNTRY PHONE NUMBER( ) YEAROFBIRTH SEX__ HIGHEST DEGREE YEAR EARNED A R MAJOR SUBJECT GRANTING INSTITUTION PRESENT POSITION -_-_- CURRENT SCIENTIFIC AREA SIGNATURE DATE *NOMINATED BY SIGNATURE OF ASM MEMBER *If you are not associated with an ASM nominating member, you can still send in this full member application form and we will contact you. Dues Ahnual dues for 1986 are $61.00, with $10.00 allocated for ASM News and $8.00 for membership services. Apply the remaining $43.00 to subscriptions for the ASM journals at the special membership rates indicated below. Journals Please check: Cl Enclosed is my dues payment (in U.S. dollars only) ...... $61 El Please send me the following ASM journal(s) at Member Price(s): U.S. Non-U.S. Amount Antimicrobial Agents and Chemotherapy $35 $49 $- Applied and Environmental Microbiology 35 49 Molecular and Cellular Biology 41 54 Infection and Immunity 41 54 International Journal of Systematic Bacteriology 35 35 Journal of Bacteriology 41 54 Journal of Clinical Microbiology 35 49 Journal of Virology 41 54 Microbiological Reviews 18 29 Total Joumal Fees $- Subtract your $43 Member Journals Credit $ -43 Subtbtal (if less than zero, enter zero) $ S Add your $61 Membership Dues $ +61 Total (DUes plus Journals). If total is less than $61.00, enter $61.00 $ PAYMENT IN U.S. DOLLARS MUST ACCOMPANY APPLICATION A membership card, voting registration form, Placement Committee form and the journal(s) of your choice will be sent within 90 days upon completion of processing. Dues for individual membership in ASM are tax deductible. Rates are for 1986 only. Non-U.S. applicants remit in U.S. dollars by check or draft payable to ASM through a U.S. bank located within the Continental U.S. Applicants from Canada may use check made out in U.S. dollars and drawn on a Canadian bank. U.S. and non-U.S. applicants may choose to pay with VISA or MasterCard. If that is your preference, please fill in the box below.

0 VISA # 0 MASTERCARD * EXPIRATION DATE TODAYS DATE SIGNATURE Minimum Charge $15.00