advanced technologies, d.o.o.
MICROBIOLOGICAL ANALYSES Is water wholesome
WWW.MICROBIUM.SI and compliant? Microbium d. o. o.
Litijska cesta 261 SI-1261 Ljubljana-Dobrunje EU, Slovenia Email: [email protected] Do raw materials pose Phone: +386 30 255 470 a risk to production processes?
How can you reduce the cost of production?
Why is it necessary to At Microbium, improve control over we offer services and the microbiological products for the effective monitoring of microbiological quality of raw materials parameters in liquids, solids and products? and air. Our own laboratory enables us to develop How to prevent new methods for detecting complaints from users? microbes according to the needs of users or to perform analyses using established What does the law methods according to the require? established ISO standards. We work with numerous Slovenian and foreign You can get the waterworks and production answer in just companies in the field of microbiological quality control. a few minutes. conducting the development the production of own microbiological of custom solutions devices for the analyses optimisation of laboratory processes
supply of laboratory custom fitting consultancy in equipment and of laboratory relation to consumables furniture legislation We have helped improve the With our experience and product quality control of drinking water in more than half of Slovenian range, we can help you to: waterworks and we understand very well the challenges that arise in the day-to-day supply of endusers with safe drinking reduce the cost of the production process, water.
significantly improve the quality of raw materials or finished products, For more than
establish effective control over water treatment,
gain a result before the water reaches the user,
achieve rapid responsiveness and fewer measures,
get results on water quality on the spot, companies, we have reduced gain reliable arguments in solving end-user complaints, the potential of microbiological risks, which is significant in improve end-user safety, and the production efficiency and financial success of the work in line with the Safe Drinking Water Plan / HAccP. companies.
SPEED COUNTS CUSTOM WE REDUCE Timely information ANALYSIS RISK on microbiological The process of detecting The timely detection of the contamination in raw microbes is adapted to presence of microbes in materials or products is of individual industrial niches. the sample implies efficient the utmost importance, as Some more complex management of the a faster analysis allows for industrial samples can production process. This faster and more effective not be successfully reduces the risk of raw action. analysed using classical material or product failure. microbiological methods. Considerable savings are In such cases, different and also found in the optimised adapted microbiological consumption of chemicals. analytical approaches are
necessary. What the quick detection and elimination of microbiological factors means for you?
Removing the safety risk from microbiological contamination
Reduction of the Reduction of number of cases of costs for carrying health issues and out analyses complaints
Risk reduction of Reduction of waste commercial detriment in contaminated the event of loss of raw materials production
Lower carbon footprint At Microbium, we deal with the following for our industrial clients:
SAMPLING
HOMOGENISATION
BIOFILM
SEPARATION PROCESSES
REMOVAL OF MICROBES
DETECTION OF MICROBES When is the rapid detection of microbes indispensable?
DRINKING AND WASTE POWDERED SAMPLES WATER TREATMENT METHODS The extensive microbiological degradation of powdered samples usually occurs gradually and changes the Monitoring individual drinking and waste water treatment properties of raw material. In such cases, action is disabled. processes is important for verifying the effectiveness of the If we detect the multiplication of bacteria in the sample in operation of the treatment plant or water treatment. time, we can optimise the process or dosage of biocides Check the concentration of bacteria remotely in a few in order to prevent the further spread of the infection and minutes using the ON-LINE device. reduce the risk of economic damage. Within one to twelve hours, the tester can reliably detect DRINKING WATER contamination in the initial development phase. The water distribution system is a living world within which the microbiological picture at the source, in the water COMPOSTS tanks and in the end-user’s system can change rapidly. It is At the maturing stage of the compost, the concentration of essential to detect the contamination in time, and then to micro-organisms should be within the envisaged limit values. take proper action in order to maintain user safety and the If they begin to multiply excessively, they can compromise positive reputation of the water supply manager. the structure and quality of the compost. The tester detects the number of bacteria in drinking Results available within 24 hours. water in 10 minutes at the site. CUSTOM DEVELOPMENT WATER-BORNE COATINGS Due to the increasingly ecological methods of extracting Water-borne coatings are susceptible to microbiological raw materials and producing finished products, they are failure, as some bacteria have developed the ability to feed increasingly sensitive to microbiological defects. Each on complex molecules found in coatings. company has specific production methods and process Using special procedures, bacteria are successfully of supplying and storing raw materials, and therefore detected within several hours even in samples that are the process of the rapid detection of bacteria must be more problematic for microbiological diagnostics. adapted and optimised according to the client’s wishes and introduced into quality control in a manner that is most favourable and friendly to the company’s policy. OIL AND PETROLEUM PRODUCTS We have developed a process for the rapid detection of Changed properties of petroleum products influence the bacteria according to the needs of the industrial client. characteristics of fuels. Today, excellent quality control in the petrochemical industry almost prevents the supply of contaminated fuel to the end-user. Companies are looking for fast and reliable methods that provide accurate and reproducible results in complex patterns. In less than an hour, the extent of microbiological contamination in a single sample is detected. Our services and products
We perform a wide range We also equip end-users or of analyses for our clients other laboratories with all using a wide range of the necessary devices and technologies in our knowledge to carry out laboratory. analyses.
We perform:
TESTS AND/OR ANALYSES OF TECHNOLOGIES WE USE DRINKING WATER • Membrane filtration E. coli / coliform bacteria Enterococci P. aeruginosa >= 24h Total number of bacteria • MPN method • Bactiquant technology (quantification of bacterial activity in 10 min) • E. coli testers and coliform bacteria P / A + incubator • Analyses according to the ISO standards • On-line microbiology analyser • Chemical parameters • Microbiological forensics (identification of sources of contamination) • Legionella
INDUSTRIAL PROCESSES • Quick testers (within a few hours) • Analyses according to ISO standards • Microbiological Forensics • Mass Spectrometry • Molecular methods (Genetics) • Comparison of microbiological resistance • Custom development
TECHNOLOGICAL WATERS • BQ mobile (10 minutes) • ATP tests • Analyses according to the ISO standards • On-line • Elimination of microbes from the water • Legionella
WASTE WATER • Fluorescence microscopy for the detection of filamentous bacteria • Detection of E. coli and enterococci • Analyses according to ISO standards • Filtration
Analyses are in accordance with the applicable regulations and legislation. We also advise companies in the field of regulation and we prepare custom solutions for them. 1. STARTER AND ADVANCED PACKAGES FOR DRINKING OR WASTE WATER
It contains everything you need to start the independent detection or quantification of E.coli and coliform bacteria without a prior knowledge of microbiology.
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thevzorec sample filterthe filter damo is prefiltriramois filtered placedna gojišče on the medium
4 3
bacterial colonies are incubate counted
2. QUICK TESTERS - BACTIQUANT® TECHNOLOGY
10 minute detection of bacteria in the samples. Verified by US EPA.
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the vzorecsample is the substrate prefiltriramofiltered is added to the filter
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the result is read using incubate the device BACTIQUANT® WATER
Technology that allows the monitoring of drinking water is reaching the end-user.
A simple, reliable and robust analytical tool for the specific detection and quantification of bacteria in drinking water and liquid samples, verified by the US Environmental Protection Agency, US EPA and one of the largest water supply systems in Europe, Vitens, NL.
Available in two versions:
WITH THE BACTIQUANT® MOBILE WITH THE BACTIQUANT® STATIONARY PORTABLE VERSION, YOU CAN IN THE LABORATORY, YOU CAN ANALYSE SAMPLES RIGHT AT QUICKLY ANALYSE DRINKING THE SAMPLING POINT. WATER SAMPLES IN LABORATORIES.
ON-LINE MEASUREMENT OF THE MICROBIOLOGICAL QUALITY OF DRINKING WATER WITH THE BACTIQUANT®ON-LINE DEVICE
BactiQuant®On-line system An automatic system for determining the presence of common bacteria in water that has been developed to optimise the process of monitoring and controlling bacteria in drinking water.
The BactiQuant®On-line is the only verified automated sampling monitoring system. It is a fully automated version of the quick testers for detecting microbial contamination. The system is based on the existing and patented BactiQuant method, which measures the activity of living bacteria in the liquid sample using highly sensitive fluorescence technology. Its advantage is that it is physically connected to the water supply network at a critical control point. Sampling and analysis of samples is carried out at predefined time intervals. After measuring the presence of common living bacteria in the sample, the device transmits the data to the central end-user system.
If the results deviate from the normal values, the system can trigger an alarm and alert the operator. 3. MICROBIUM COLOR
Simple chromogenic testers for the detection of Escherichia coli and coliform bacteria without using a UV lamp. 1 2 3 4 In accordance with the Rules on Drinking Water, they can be used for the internal control of drinking water (also chlorinated).
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the sample (100 ml) add/shake the is poured into powdered a bottle substrate 1 - free from bacteria 2 - a high number of aerobic bacteria 3 - coliform bacteria 4 3 4 - E. coli
the result is read (colour change) incubate
4. CHEMICAL PARAMETERS 5. MICROBIOLOGICAL FORENSICS
Testing a drinking water sample with individual On the basis of sample analyses that we perform chemical parameters shows the extent and degree for clients on products, in production processes and of contamination of drinking water with chemical water resources, we can follow the contamination substances that may pose a risk to human health. and determine the source by monitoring the bacteria. Millions of species of different bacteria create a trace In our laboratory, we can perform the determination that we can connect to sequences, thus helping to of chemical parameters or provide the client with upgrade the processes. all the necessary materials and equipment for an independent analysis. 6. COMPARISON OF MICROBIOLOGIAL RESISTANCE (ANTIBIOGRAMS) WITH ANTIMICROBIAL AGENTS/BIOCIDE
We check the resistance of a particular microbial strain to a specific antimicrobial agent.
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We add a disk We enrich We transfer with different concentrations of We incubate We interpret the bacterial it to the the antimicrobial the antibiogram the results culture medium agent
7. ATP TESTS
A quick and reliable method for detecting the presence of micro-organisms based on the microbial production of ATP molecules in technological waters.
8. ELIMINATION
In an environmentally-friendly way, microorganisms are removed from the plumbing or production processes in companies or even completely removed from various types of waters.
We use:
Sonocavitation devices High ionizing radiation for the complete natural for the removal of Treatment with UV elimination of bacteria bacteria from already devices for different in reservoirs prepared products types of water 9. LEGIONELLA
By using fluorescence microscopy and specific antibodies and cultivation methods, we can detect and quantify the legionella in a sample within three instead of ten days.
10. THE DETECTION OF FILAMENTOUS BACTERIA IN WASTE WATER
Using fluorescence microscopy and specific dyes, we take a sample and evaluate the concentration of filamentous bacteria using a camera.
11. ANALYSES ACCORDING TO THE ISO STANDARDS
In accordance with the Rules on Drinking Water and the Decree on the Emission of Substances and Heat when Discharging Waste Water into Waters and the Public Sewage System, we perform microbiological tests of drinking and waste water for the specific needs of companies and waterworks.
DRINKING WATERS:
STANDARD NUMBER SUBJECT SIST EN ISO 9308-1 Detection of E. coli and coliform bacteria in drinking water using membrane filtration
SIST EN ISO 7899-2 Detection of faecal enterococci in drinking water using membrane filtration
SIST EN ISO 6222 Detection of the total viable microorganisms using the colony count method
WASTE WATERS:
STANDARD NUMBER SUBJECT SIST EN ISO 9308-3 Detection of E. coli in waste water using the MPN * method. “Most Probable Number”
SIST EN ISO 7899-1 Detection of faecal enterococci in waste water using the MPN method
*Most Probable Number Method 12. CUSTOM DEVELOPMENT 13. CUSTOM LABORATORY AND OUR INNOVATIONS FURNITURE
We are developing testers/methods for the rapid detection Laboratory furniture has surfaces resistant to chemicals of bacteria and smart laboratory devices that automate and is designed to optimise laboratory work. It combines laboratory work. Thus we make it easier to work in the the robustness of workmanship with precision work, since laboratory and shorten the time of analyses. individual elements can move on slides and enable the rapid preparation of the laboratory for efficient work. It is designed so that the elements do not touch the ground, which makes QUICK TESTERS/METHODS FOR it easy to clean the laboratory. THE DETECTION OF BACTERIA IN COMPLEX SAMPLES Our experts will take care of the design, manufacture and installation of high-quality laboratory furniture. They enable the detection of live bacteria in complex samples within a few hours. WE INVITE YOU TO VIEW THE FURNITURE AT OUR FACILITY
MICROBIUM SMART INCUBATOR
This enables the automatic recognition of E. coli colonies and coliform bacteria and/or colour changes of certain testers.
The incubator combines several different technologies and, in accordance with the protocols, sends test results to a smart device.
14. LABORATORY EQUIPMENT
With a wide range of laboratory equipment, clients can fully equip themselves for independent laboratory work and microbiological analysis.
In addition to supplying equipment for our clients, we also conduct training on proper and safe use and proper techniques for successful work.
Razvoj poteka v sodelovanju s podjetjem Domel. Microbes cannot be seen with the naked eye, but they can be found everywhere around us. They play an important role in our ecosystem, but unfortunately they can be a very disrupting factor in many areas or even pose a threat. At that point, fast, simple and reliable microbiological diagnostics is the only right approach. Contact us!
At Microbium, we keep pace with the latest trends and the Contact us at [email protected] or call us at +386 30 255 470 development of microbiology. We transfer our knowledge and we will be happy to advise you on your and experience to practice wherever microbiological tests challenges in the field of microbiology and are required. With our own research and development water analysis. of products and services, we bring useful solutions to organisations, increase the added value of their products and improve productivity in their organisation.
14. LABORATORY EQUIPMENT Gregor Zupin, CEO
We will find the fastest, most reliable and financially viable solution to your challenges. WWW.MICROBIUM.SI Microbium d. o. o. Litijska cesta 261 SI-1261 Ljubljana-Dobrunje EU, Slovenia Email: [email protected] Phone: +386 30 255 470