Author Manuscript Published OnlineFirst on April 23, 2013; DOI: 10.1158/0008-5472.CAN-12-3386 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. RXRα represses NRF2-mediated transcription Title: RXRα Inhibits the NRF2-ARE Signalling Pathway Through A Direct Interaction With the Neh7 domain of NRF2 Running title: RXRα represses NRF2-mediated transcription 1,2 2 1 2 1 1 Hongyan Wang , Kaihua Liu , Miao Geng , Peng Gao , Xiaoyuan Wu , Yan Hai , Yangxia Li1, Yulong Li1, Lin Luo2, John D. Hayes3, Xiu Jun Wang2† and Xiuwen Tang1† 1Department of Biochemistry and Genetics, 2Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, PR China, 310058, 3Division of Cancer Research, Medical Research Institute, Ninewells Hospital & Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom Key words: RXRα, repressor, NRF2, ARE, drug resistance †To whom correspondence should be addressed: PO BOX 18, The School of Medicine, Zhejiang University, Hangzhou, PR China 310058. Tel: +0086-571- 88208266; Fax: +0086-571-88208266; E-mail:
[email protected] (Xiu Jun Wang) or
[email protected] (Xiuwen Tang) Word count: 4993 Total number of figures and tables: 6 The authors disclose no potential conflicts of interest. Abbreviations: AKR1C, aldo-keto reductases 1C1 and 1C2; ARE, antioxidant response element; ATF3, activating transcription factor 3; BHA, butylated hydroxyanisole; CAR, constitutive androstane receptor; CBP, CREB, cAMP response element binding protein, binding