TaqMan® Gene Signature Arrays

TaqMan® Human Angiogenesis Array

This array is part of a collection of TaqMan® Gene Signature Group Assays Gene Symbols ® Arrays that enable analysis of hundreds of TaqMan Gene Angiogenic growth factor 28 ANG, ANGPT1, ANGPT2, ANGPT4, CSF3, Expression Assays on a micro fluidic card with minimal effort. CTGF, CXCL12, ECGF1, EDIL3, EPHB2, FGF1, FGF2, FGF4, FST, GRN, HGF, IL8, Angiogenesis is the process of developing new blood vessels LEP, MDK, PDGFB, PROK1, PTN, TGFA, TGFB1, TNF, VEGF, VEGFB, VEGFC from pre-existing ones. It depends on cell adhesion and proteolytic mechanisms that involve the activity of growth Angiogenesis inhibitor 37 ADAMTS1, ANGPTL1, BAI1, CHGA, COL15A1, COL18A1, COL4A1, COL4A2, factors, extracellular matrix proteins, proteases and adhesion COL4A3, CXCL10, CXCL2, F2, FBLN5, molecules. The development of new vessels requires FGA, FLT1, FN1, HSPG2, IFNB1, IFNG, coordinated regulation of processes depending on a balance IL12A, ITGA4, LECT1, PF4, PLG, PRL, SEMA3F, SERPINB5, SERPINC1, of activators and inhibitors. Angiogenesis is important for SERPINF1, THBS1, THBS2, TIMP2, physiologic processes such as development, morphogenesis, TIMP3, TNFSF15, TNMD, TNNI1, VASH1 wound repair and reproduction. Uncontrolled angiogenesis Angiogenesis marker 10 AMOT, ANGPTL2, ANGPTL3, may contribute to disease mechanisms associated with ANGPTL4, CEACAM1, HEY1, ITGAV, PECAM1, PGK1, XLKD1 macular degeneration, psoriasis, rheumatoid arthritis, tumor growth and metastasis. Angiogenesis target 14 EDG1, ENPP2, FLT3, FLT4, ITGB3, KDR, KIT, NRP1,NRP2, Higher eukaryotes have two vascular systems: blood and PDGFRA, PDGFRB, TEK, TIE1 lymphatic. The blood vasculature transports oxygen, carbon Lymphangiogenesis marker 5 CD44, CDH5, FIGF, FOXC2, PROX1 dioxide, nutrients, metabolic products, cells of the immune Controls 2 18S, GAPDH system, hormones and other factors. The lymphatic vessels collect and transport protein-rich interstitial fluid via lymph References: nodes and lymphatics back into the blood circulation. The Ambesi, A. et al. Cancer Res. 2005 Jan 1, 65(1):148–56 lymphatic system is important in normal physiologic processes Brooks, P.C. et al. Cell 1998 Feb 6, 92(3):391–400 such as immunoregulation and tissue repair. It also plays a role in the pathology of chronic inflammation, lymphedema Folkman, J. Ann. Rev. Med. 2006, 57:1–18 and tumor metastasis. Lymphangiogenesis is the formation Gonzalez, E.M. et al. J. Biol. Chem. 2005, 280(8):7080–7087 of lymphatic vessels from pre-existing ones, analogous Kaur, B. et al. Oncogene 2005, 24(22):3632–42 to angiogenesis. Lee, T. et al. J. Biol. Chem. 1998, 273(44):28805–812 This TaqMan® Gene Signature Array contains 94 assays and Nyberg, P. et al. Cancer Res. 2005, 65:3967–79 two controls to study angiogenesis and lymphangiogenesis in OReilly, M.S. et al. Science 1999, 285:1296–98 humans. It includes known angiogenesis growth factors like Pike, S.E. et al. J Exp Med. 1998 Dec 21, 188(12):2349–56 VEGF, and known matrix derived inhibitors such as endostatin. Additionally, the array contains markers and targets for Rege, T.A. et al. Neuro-oncol. 2005 Apr, 7(2):106–21 angiogenesis and lymphangiogenesis. Staton, C.A. et al. Blood 2004 Jan 15, 103(2):601–6 Struman, I. et al. PNAS 1999, 96:1246–51 Tabruyn, S. et al. Molec. Endoc. 2006, 19(7):1932–42 Tobler, N.E. & Detmar, M. J Leukoc Biol. 2006, 80(4):691–6 Witte, M.H. et al. Cancer Metastasis Rev. 2006, 25:159–184 Yi, M. & Ruoslahti, E. PNAS 2001, 98:620–24 TaqMan® Gene Signature Arrays

Gene Signature Array Name # of Targets/Controls Format Pack Size Part Number

Human Angiogenesis Array 94/2 Format 96a 4 arrays/pack 4378710

Human Angiogenesis Array

A FGA PLG SERPINC1 PRL MMP2 ANG ANGPT1 ANGPT2 CXCL12 EDIL3 18S EPHB2 FGF1 FGF2 FGF4 FST HGF IL8 LEP MDK ECGF1 PDGFB PTN PROK1 1 B TGFA TGFB1 TNF VEGF VEGFB VEGFC CTGF FBLN5 THBS1 TNFSF15 ITGA4 IFNB1 IFNG CXCL10 IL12A SERPINF1 PF4 VASH1 ADAMTS1 ANGPTL1 AMOT CD44 CDH5 CXCL2 C SERPINB5 FLT1 SEMA3F TEK TIE1 TNMD TIMP2 TIMP3 ANGPTL2 ANGPTL3 CEACAM1 HEY1 ITGAV PECAM1 PGK1 XLKD1 KIT FLT3 NRP2 KDR ENPP2 FIGF FOXC2 COL4A1 2 D COL4A2 COL15A1 HSPG2 COL18A1 FN1 COL4A3 F2 BAI1 CHGA ANGPT4 CSF3 GRN THBS2 LECT1 TNNI1 ANGPTL4 ITGB3 PDGFRA PDGFRB FLT4 NRP1 EDG1 PROX1 GAPDH E FGA PLG SERPINC1 PRL MMP2 ANG ANGPT1 ANGPT2 CXCL12 EDIL3 18S EPHB2 FGF1 FGF2 FGF4 FST HGF IL8 LEP MDK ECGF1 PDGFB PTN PROK1 3 F TGFA TGFB1 TNF VEGF VEGFB VEGFC CTGF FBLN5 THBS1 TNFSF15 ITGA4 IFNB1 IFNG CXCL10 IL12A SERPINF1 PF4 VASH1 ADAMTS1 ANGPTL1 AMOT CD44 CDH5 CXCL2 G SERPINB5 FLT1 SEMA3F TEK TIE1 TNMD TIMP2 TIMP3 ANGPTL2 ANGPTL3 CEACAM1 HEY1 ITGAV PECAM1 PGK1 XLKD1 KIT FLT3 NRP2 KDR ENPP2 FIGF FOXC2 COL4A1 4 H COL4A2 COL15A1 HSPG2 COL18A1 FN1 COL4A3 F2 BAI1 CHGA ANGPT4 CSF3 GRN THBS2 LECT1 TNNI1 ANGPTL4 ITGB3 PDGFRA PDGFRB FLT4 NRP1 EDG1 PROX1 GAPDH

I FGA PLG SERPINC1 PRL MMP2 ANG ANGPT1 ANGPT2 CXCL12 EDIL3 18S EPHB2 FGF1 FGF2 FGF4 FST HGF IL8 LEP MDK ECGF1 PDGFB PTN PROK1 5 J TGFA TGFB1 TNF VEGF VEGFB VEGFC CTGF FBLN5 THBS1 TNFSF15 ITGA4 IFNB1 IFNG CXCL10 IL12A SERPINF1 PF4 VASH1 ADAMTS1 ANGPTL1 AMOT CD44 CDH5 CXCL2 K SERPINB5 FLT1 SEMA3F TEK TIE1 TNMD TIMP2 TIMP3 ANGPTL2 ANGPTL3 CEACAM1 HEY1 ITGAV PECAM1 PGK1 XLKD1 KIT FLT3 NRP2 KDR ENPP2 FIGF FOXC2 COL4A1 6 L COL4A2 COL15A1 HSPG2 COL18A1 FN1 COL4A3 F2 BAI1 CHGA ANGPT4 CSF3 GRN THBS2 LECT1 TNNI1 ANGPTL4 ITGB3 PDGFRA PDGFRB FLT4 NRP1 EDG1 PROX1 GAPDH M FGA PLG SERPINC1 PRL MMP2 ANG ANGPT1 ANGPT2 CXCL12 EDIL3 18S EPHB2 FGF1 FGF2 FGF4 FST HGF IL8 LEP MDK ECGF1 PDGFB PTN PROK1 7 N TGFA TGFB1 TNF VEGF VEGFB VEGFC CTGF FBLN5 THBS1 TNFSF15 ITGA4 IFNB1 IFNG CXCL10 IL12A SERPINF1 PF4 VASH1 ADAMTS1 ANGPTL1 AMOT CD44 CDH5 CXCL2 O SERPINB5 FLT1 SEMA3F TEK TIE1 TNMD TIMP2 TIMP3 ANGPTL2 ANGPTL3 CEACAM1 HEY1 ITGAV PECAM1 PGK1 XLKD1 KIT FLT3 NRP2 KDR ENPP2 FIGF FOXC2 COL4A1 8 P COL4A2 COL15A1 HSPG2 COL18A1 FN1 COL4A3 F2 BAI1 CHGA ANGPT4 CSF3 GRN THBS2 LECT1 TNNI1 ANGPTL4 ITGB3 PDGFRA PDGFRB FLT4 NRP1 EDG1 PROX1 GAPDH

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For Research Use Only. Not for use in diagnostic procedures. Practice of the patented 5’ Nuclease Process requires a license from Applied Biosystems. Purchase of the TaqMan® Human Angiogenesis Array includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of an Authorized 5’ Nuclease Core Kit. No other patent rights are conveyed expressly, by implication, or by estoppel. For further information on purchasing licenses contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The TaqMan® Array is covered by U.S. Patents Nos. 6,514,750, 6,942,837, 7,211,443, and 7,235,406. Micro Fluidic Card developed in collaboration with 3M Company. © Copyright 2008. Applied Biosystems. All rights reserved. Applera, Applied Biosystems, and AB (Design) are registered trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 03/2008 Publication 127MI61-02

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