Mouse and Human Dendritic Cell Subsets

Common Monocyte Progenitor (cMoP) Common Dendritic Granulocyte-Macrophage Pre-DC Progenitor (CDP) Pre-DC Progenitor (GMP) Human Equivalent of MDP or CDP

Common Monocyte Progenitor (cMoP) Plasmacytoid DC Plasmacytoid DC Pre-DC Blood DC

Monocyte Plasmacytoid DC Plasmacytoid DC

Monocyte Blood Monocyte Blood Monocyte

Pre-DC

Mouse Skin DCs

Langerhans Cell Integrin αE/CD103+ CD11b– Human Skin DCs Unique origin from a yolk sac-derived erythromyeloid + progenitor cell or a fetal liver-derived monocyte CD207 Dermal DC rather than a bone marrow-derived preDC. Cell Surface Markers CD4– EpCAM/TROP1–/low Cell Surface Markers CD8α– F4/80– Langerhans Cell + F4/80+ CD11c CD11c+ Integrin αE/CD103+ If similar to mouse, human Langerhans cells originate from yolk sac-derived + Integrin αE/CD103– CD24 + Integrin αM/CD11b–/low erythromyeloid progenitor cells or fetal liver-derived monocytes. – + CD24 CLEC9a Integrin αM/CD11b + + + CLEC9a Langerin/CD207 Cell Surface Markers + Langerin/CD207 – +/– DC-SIGN/CD209 – MHC class II+ Lin– CD14 Integrin αM/CD11b + + CX3CR1 DEC-205/CD205 MHC class II – α – + E-Cadherin+ Langerin/CD207+ + DC-SIGN/CD209 SIRP /CD172a CD1a + SIRPα/CD172a + + EpCAM/TROP1 DEC-205/CD205+ XCR1+ CD1c/BDCA-1+ EpCAM/TROP1 SIRPα/CD172a Mouse Lymphoid CD11c+ HLA-DR+ Integrin αE/CD103– CD11b– CD207+ Dermal DC CD14+ Dermal DCs Tissue DCs Cell Surface Markers Cell Surface Markers – + + CD24+ Lin CD14 HLA-DR – – – – –/+ – EpCAM/TROP1–/low Integrin αE/CD103 CD11b CD207 Dermal DC CD1a CD163 Langerin/CD207 +/– + + Integrin αE/CD103– CD1c/BDCA-1 DC-SIGN/CD209 CD8α Classical DC Cell Surface Markers + – –/low – – CD11c EpCAM/TROP1 Cell Surface Markers Intracellular Markers Integrin αM/CD11b CD24 Langerin/CD207 + – + CD4– IGSF4A/SynCAM1/Necl2+ Batf3+ Langerin/CD207 EpCAM/TROP1 MHC class II + + +/– + – α –/low CD1a CD1c/BDCA-1 Dermal DCs CD8α+ Integrin αE/CD103 IRF8+ MHC class II Integrin αE/CD103 SIRP /CD172a – + Integrin αM/CD11b– SIRPα/CD172a Integrin αM/CD11b–/low Cell Surface Markers CD11c – –/low α high + +/– Secreted Molecules Lin CD141/BDCA-3 Integrin M/CD11b CD24 Langerin/CD207 – – IL-12 + CD163 Langerin/CD207 + MHC class II+ CD1a CLEC9a – + – + DC-SIGN/CD209– SIRPα/CD172a+ – SIRPα/CD172a– Integrin αE/CD103 CD11b CD207 Dermal DC CD1c/BDCA-1 DC-SIGN/CD209 + EpCAM/TROP1– + XCR1+ CD11c DEC-205/CD205 Cell Surface Markers – + – + + CD14 HLA-DR F4/80– CD4 CX3CR1 Integrin αM/CD11b CD8α– EpCAM/TROP1– Langerin/CD207– + CD11b Classical DC CD11c+ F4/80+ MHC class II+ + + Cell Surface Markers Secreted Molecules CD24– Fcγ RI/CD64 SIRPα/CD172a + + + – – CD1a CD141/BDCA-3 Dermal DCs CD4+/– F4/80 IFN-γ CLEC9a– Integrin αE/CD103 XCR1 α– IGSF4A/SynCAM1/Necl2– Cell Surface Markers CD8 IL-2 – DC-SIGN/CD209– + Integrin αM/CD11b+ Pre-DC Lin CD11c IL-6 + EpCAM/TROP1– + Langerin/CD207– CD1a CD24 low/int HLA-DR+ – MHC class II+ CD1c/BDCA-1 CLEC9a + low IGSF4A/SynCAM1/Necl2+ + SIRPα/CD172a+ CD16 Monocyte CD11c DCIR2 – Integrin αM/CD11bhigh + – CD14 DC-SIGN/CD209 XCR1 – CD141/BDCA-3+ Langerin/CD207 DEC-205/CD205+ CD163– SIRPα/CD172a+ + Plasmacytoid DC CLEC9a+ XCR1 Cell Surface Markers Secreted Molecules B220/CD45 R+ MHC class II+ IFN-α Bst-2/PDCA-1+ Siglec-H+ IFN-β Human Secondary CD11c+ SIRPα/CD172+ IL-6 CLEC9a+ TNF-α Lymphoid Organs/Non-Lymphoid + Intracellular Markers DC-SIGN/CD209 TLR7+ – Dendritic Cells (DCs) provide a crucial Tissue Parenchyma DCs Integrin αM/CD11b TLR9+ + Ly6C link between the innate and adaptive immune response. This occurs primarily CD141/BDCA-3+ Classical DC (CD8α+-like DC) Cell Surface Markers Intracellular Markers Secreted Molecules through their ability to capture, process, Lin– Batf3+ IL-12 CD1a– ID2+ CD1c/BDCA-1– IRF8+ and present antigens to T cells, and CD11c+ CD141/BDCA-3+ regulate T cell functions in immunity CLEC9a+ Mouse Intestinal DEC-205/CD205high Plasmacytoid DC and tolerance. This illustration highlights HLA-DR+ Cell Surface Markers Intracellular Markers Lamina Propria/Peyer’s Patch DCs IGSF4A/SynCAM1/Necl2+ Lin– TLR7+ Integrin αM/CD11blow CD1a– TLR9+ distinct DC subsets that exist in both + low XCR1 CD11c Secreted Molecules DLEC/CLEC4C/BDCA-2+ IFN-α + – human and mouse. The different DC HLA-DR+ α IFN-β Integrin E/CD103 CD11b Classical DC IL-3 Rα/CD123+ IL-6 Cell Surface Markers Neuropilin-1/BDCA-4+ – + subsets respond to distinct self and α CD4– DC-SIGN/CD209 Langerin/CD207 TNF- Human Blood DCs CD8α– DEC-205/CD205+ MHC class II+ + – – CD11c EpCAM/TROP1 SIRPα/CD172a foreign antigen challenges, and exhibit + + CD24+ F4/80– XCR1+ CD1c/BDCA-1 Classical DC (CD11b -like DC) + CLEC9a+ Integrin αE/CD103 different functional specialization. These Cell Surface Markers Secreted Molecules + + – – α CX3CR1– Integrin αM/CD11b Lin IL-1β CD141/BDCA-3 Classical DC (CD8 -like DC) CD1a– IL-6 Cell Surface Markers Secreted Molecules subtypes can be characterized based CD1c/BDCA-1+ IL-10 Lin– IGSF4A/SynCAM1/Necl2+ IL-12 Plasmacytoid DC CD11c+ IL-12 CD1a– Integrin αM/CD11blow Integrin αE/CD103– CD11b+ Cell Surface Markers Intracellular Markers on their tissue distribution, cell surface CD141/BDCA-3+/– TNF-α CD1c/BDCA-1– XCR1+ + Classical DC B220/CD45 R+ TLR7+ HLA-DR CD11c+ Intracellular Markers + + α low + Bst-2/PDCA-1 TLR9 markers, and transcriptional programs. Integrin M/CD11b CD141/BDCA-3 Batf3+ Cell Surface Markers + + CLEC9a + – Fcγ RI/CD64+ CD11c ID2 CD4 + Secreted Molecules high – α – CLEC9a DEC-205/CD205 IRF8+ CD8α Integrin E/CD103 IFN-α + + HLA-DR + Integrin αM/CD11b+ DC-SIGN/CD209 CD11c – IFN-β +/– Langerin/CD207– Integrin αM/CD11b CD24 + IL-6 – MHC class II+ Ly6C + + CLEC9a + TNF-α CD1c/BDCA-1 Classical DC (CD11b -like DC) CX3CR1+ SIRPα/CD172a+ MHC class II – + Cell Surface Markers Secreted Molecules – XCR1 Siglec-H – EpCAM/TROP1 + Lin IL-1β + SIRPα/CD172a F4/80 CD1a– IL-6 CD1c/BDCA-1+ IL-10 CD11c+ IL-12 Mouse Lung/Liver/Kidney DCs CD141/BDCA-3+/– TNF-α Integrin αE/CD103+ CD11b+ Classical DC HLA-DR+ α low Cell Surface Markers Integrin M/CD11b CD4– DC-SIGN/CD209+ MHC class II+ Integrin αE/CD103+ CD11b– Classical DC CD8α– EpCAM/TROP1– SIRPα/CD172a– + – – Cell Surface Markers CD11c F4/80 XCR1 – DC-SIGN/CD209– Langerin/CD207+ + CD4 + Integrin αE/CD103 + + Plasmacytoid DC CD24 CD8α– DEC-205/CD205 MHC class II – + CLEC9a Integrin αM/CD11b + – – Cell Surface Markers Intracellular Markers CD11c EpCAM/TROP1 SIRPα/CD172a – + – – Lin TLR7 CX3CR1 Langerin/CD207 + F4/80– XCR1+ CD24 CD1a– TLR9+ + Integrin αE/CD103+ CLEC9a CD11clow – Integrin αM/CD11b– Secreted Molecules CX3CR1 DLEC/CLEC4C/BDCA-2+ IFN-α HLA-DR+ – + IFN-β α IL-3 Rα/CD123+ Integrin E/CD103 CD11b Classical DC IL-6 Neuropilin-1/BDCA-4+ Cell Surface Markers TNF-α CD4– EpCAM/TROP1– MHC class II+ CD8α– F4/80+ SIRPα/CD172a+ Human Inflammatory DCs CD11c+ Fcγ RI/CD64+ XCR1– CD24+/– Integrin αE/CD103– in Peripheral Tissues & Draining Mouse Inflammatory DCs CLEC9a– Integrin αM/CD11b+ in Peripheral Tissues & Draining CX3CR1+ Langerin/CD207– Lymph Nodes During Inflammation Plasmacytoid DC Lymph Nodes During Inflammation Cell Surface Markers Intracellular Markers B220/CD45 R+ TLR7+ Bst-2/PDCA-1+ TLR9+ Inflammatory DC + Absent from steady state lymphoid organs and tissues; CD11c Secreted Molecules CLEC9a+ Inflammatory DCs differentiate from monocytes that are IFN-α DC-SIGN/CD209+ recruited to sites of inflammation. IFN-β Inflammatory DC Integrin αM/CD11b– IL-6 Cell Surface Markers Secreted Molecules Absent from steady state lymphoid organs and tissues; Ly6C+ – TNF-α Lin IL-23 Inflammatory DCs differentiate from monocytes that are MHC class II+ CD1a+ NO recruited to sites of inflammation. Siglec-H+ CD1c/BDCA-1+ TNF-α Cell Surface Markers Secreted Molecules SIRPα/CD172a+ CD11c+ CD11c+ IL-12 CD14+ CD107b/Mac-3+ NO Fcε RIα+ F4/80+ TNF-α Fcγ RI/CD64+ Fcγ RI/CD64+ HLA-DR+ Fcε RI+ Integrin αM/CD11b+/– Integrin αM/CD11b+ MMR/CD206+ Ly6C+ SIRPα/CD172a+ MHC class II+ MMR/CD206+

NOTE: This poster conveys a general overview and should be considered neither comprehensive nor definitive. The details of this information are understood to be subject to interpretation. © 2017 R&D Systems

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