Guide to Human CD Antigens

Home , ART4, CD1, CD109, CD133, CD134, CD153

Discover more at abcam.com/CD About Abcam

Abcam is a provider of protein research tools and services, with an unrivaled range of products and expert technical support, enabling scientists to analyze living cells at the molecular level and improving the understanding of health and disease.

Abcam is committed to providing scientists with an extensive choice of reagents and tools, with the most comprehensive, honest and up-to-date datasheets and customer reviews, fast delivery and helpful customer service & technical support. The company’s catalog of products includes primary and secondary antibodies, proteins, peptides, lysates, biochemicals, immunoassays and other kits.

To find out more, please visit www.abcam.com

A note on CD antigens

The cluster of differentiation (CD) nomenclature system was conceived to classify antigens found on the surface of leukocytes. Initially, surface antigens were named after the monoclonal antibodies that bound to them. As there were often multiple monoclonal antibodies raised against each antigen by different labs, the need arose to adopt a consistent nomenclature. The current system was adopted in 1982, during the 1st International Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA) in Paris.

Under the current naming system, antigens that are well characterized are assigned an arbitrary number (e.g. CD1, CD2 etc) whereas molecules that are recognised by just one monoclonal antibody are given the provisional designation “CDw”. Physiologically, CD antigens do not belong in any particular class of molecules, with their functions ranging from cell surface receptions to adhesion molecules. Although initially used for just human leukocytes, the CD molecule naming convention has now been expanded to cover both other species (e.g. mouse) as well as other cell types. Human CD antigens are currently numbered up to CD363.

The presence or absence of a specific antigen from the surface of particular cell population is denoted with “+” or “-“ respectively. Varying cellular expression levels are also marked as hi or low , for example central memory T cells are CD62L hi whereas effector memory T cells are CD62L low . Monitoring the expression profiles of different CD Abbreviations antigens has permitted the identification, isolation and phenotyping of cell types according to their function in various immune processes. ADCC Antibody-dependent cell-mediated cytotoxicity APC Allophycocyanin FITC Fluorescein Isothiocyanate NFkB NF-kappa-B Disclaimers OC 515™ Orange Cytognos 515™ PBMCs Peripheral blood mononuclear cells OC 515™ is a trademark of Cytognos S.L. PerCP Perinidin Chlorophyll Protein CF™ is a trademark of Biotium, Inc PE Phycoerythrin Cy™ is a trademark of GE healthcare Monoclonal s t i k

e l y a m d a 7 n n o e i 7 5 s r M t o ™ h e s l ™ a 5 t P n 5 c ™ ™ i y a c g 0 C o C t o 1 y y C E r y r u o 4 j C T l r o P 5 o / I i P C C P n

n o e / / u A l F ™ u f o B C

P C P E E

F c r P m P P O n Target Alternative Cellular Functions e C A h t m U I

name name expression O

CD1a - Cortical thymocytes, Langerhans cells, dendritic cells and Non-peptide antigen presentation to T-cell receptors on NKT cells, marks • • • • • • certain T-cell leukemias. T-cells at the short cortical thymic stage of differentiation. • • • CD1b - Cortical thymocytes, Langerhans cells and dendritic cells. Non peptide antigen presentation to T-cell receptors on NKT cells. • • • • CD1c - Cortical thymocytes, Langerhans cells, dendritic cells and Non peptide antigen presentation to T-cell receptors on NKT cells. • • • B-cell subsets. CD1d R3G1 Intestinal epithelial cells, B-cell subsets, cortical thymocytes, Non peptide antigen presentation to T-cell receptors on NKT cells. • • • • • • dendritic cells and certain T-cell leukemias. CD1e R2G1 Cortical thymocytes, Langerhans cells, dendritic cells and Non peptide antigen presentation. • certain T-cell leukemias. CD2 SRBC, Erythrocyte receptor, LFA-2, Thymocytes, natural killer cells, T- and B-cells. Adhesion between T-cells and other cell types and T-cell activation. • • • • • • • • • • • LFA-3 receptor, Rosette receptor CD3d T3D, OKT3 Mature T-cells and thymocytes. T-cell activation signaling and regulation of TCR expression. •* •* •* •* •* •* •* •* •* •* •* •* •* CD3e T3E, TCRE Mature T-cells and thymocytes. T-cell activation signaling and regulation of TCR expression. • • • • • • • • • CD3g T3G Mature T-cells and thymocytes. T-cell activation signaling and regulation of TCR expression. • • • CD4 - Thymocyte subsets, T-helper cells, regulatory T-cells, T-cell activation, thymic differentiation and receptor for HIV. • • • • • • • • • • • • • monocytes and macrophages. CD5 LEU1 Thymocytes, T- and B-cell subsets and B-cell chronic Regulates T-cell: B-cell interactions. Interacts with CD72. • • • • • • • • • • • • lymphocytic leukemia cells. CD6 OX52, T12, TP120 Thymocytes, T- and B-cell subsets and neuron subsets. Thymocyte development, a potential market of T-cell activation. • • • • • • • • • CD7 LEU 9, GP40, TP41 Thymocytes, T-cells, natural killer cells and pluripotent T-cell costimulation. Interacts with SECTM1. • • • • • • • hematopoietic stem cells. CD8a MAL Thymocyte subsets, cytotoxic T-cells, natural killer cells and Co-receptor for MHC class 1 molecules. • • • • • • • • • • • • • dendritic cells subsets. CD8b CD8B1 Thymocyte subsets and cytotoxic T-cells. Co-receptor for MHC class 1 molecules. • • • • • • • • CD9 P24, 5H9 antigen, MIC3, Platelets, pre-B-cells, eosinophils, basophils, activated T-cells, Cell adhesion and migration, platelet activation and aggregation. • • • • • • • • • TSPAN29, GIG2 endothelial cells and epithelial cells. CD10 Neprilysin, CALLA, SFE, MME, EPN B- and T-cell precursors, fibroblasts, neurons and bone Peptidase, regulates B-cell growth. • • • • • • • • • • marrow stromal cells. CD11a Integrin alpha-L, LFA-1A, ITGAL Leukocytes, lymphocytes, granulocytes, monocytes Intracellular adhesion and costimulation. • • • • • • • • • • • and macrophages. CD11b Integrin alpha-M, CR3A, ITGAM Granulocytes, monocytes, natural killer cells, T- and B-cells Cell adhesion, apoptosis and chemotaxis. • • • • • • • • • • • • • • and dendritic cells. CD11c Integrin alpha-X, Leu M5, ITGAX Monocytes, macrophages, granulocytes, T- and B-cell subsets. Cell adhesion. • • • • • • • • CD11d Integrin-alpha-D, ADB2, ITGAD Myelomonocytic cells, peripheral blood leukocyte subsets and Receptor for ICAM3 and VCAM1. May play a role in atherosclerotic tissue-specialized cells. process and phagocytosis of blood-borne pathogens. CD13 Aminopeptidase N, ANPEP, APN, Granulocytes, monocytes (+ precursors), endothelial cells Zinc binding metalloproteinase. • • • • • • • • PEPN and epithelial cells. CD14 - Monocytes, macrophages (myelomonocytic cells), Receptor for complex of LPS and LBP. • • • • • • • • • • • • • • Langerhans cells and granulocytes. CD15 3-FAL, ELAM 1 ligand, Granulocytes, monocytes, neutrophils and eosinophils. Cell adhesion. Mediates phagocytosis and chemotaxis. • • • • • • • • • fucosyltransferase, Leu M1 CD16a Fc-gamma RIII-alpha, FCGR3A, Neutrophils, natural killer cells and macrophages. Low affinity Fc receptor. Mediates phagocytosis and ADCC. • • • • • • • • • FCG3, FCGR3, IGFR3 CD16b Fc-gamma RIII-beta, FCGR3B, Neutrophils and stimulated eosinophils. Low affinity Fc receptor. • FCG3, FCGR3, IGFR3

Discover more at abcam.com/CD Key *: against pan-CD3 : Featured product C C C C C C C n T C C C C C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D r m g 1 3 2 3 2 2 2 2 1 2 3 2 1 2 2 2 3 3 3 4 3 3 3 3 e e 9 4 4 7 8 2 9 0 8 5 0 1 7 3 7 6 5 2 8 0 9 6 1 3 t

- -

T T G C S m T T A I F a E L T C T S I B B I n I N A T C B C F L L r T D r A T N P P S t r L n n n e e e a n e a a o N N c N C P N N - N - p I D p I l D l l i - e 3 3 o i o T P 2 t t t o t c c a a a c p G G l l t n e e e

t u m c w e y 3 s 4 c D m b m y r F F F E F F F P g e e R i A l P 3 t t l e e g e t s a g g r i m - r e e L L t 5 c 4 t

/ m o a p p B l R R R

e e 1 n R R D l p

B l A a C e p p e a c s r r r r r l l o E E , l r

s e e m t t p i i

e e e i 6

a a t , i u f p n S S S l l n n u d n o o 2 , a P p , c L s 4 b

i e e f y C C t t

d G c c c , t c i l a

C a T , - r r , s r m

F F F e n u i h b o e l m m L b b g e

o v e e e B c

y M i 3 2 i A i n e s A

C d P s u i

s n n , 5 8

r o o e e B e e l n e l c t p p p a r

7 , e y a e e

f u

y - D y S k C P

4 - e , n L m p

u c 1

t t d r a t t t b

L B c

, , 2 r n n

a a b l o o o o a R K

c t

e 4 i

C r E y - , o

c o i G A C e m 2

p t t c L

- - a n

u 1 r r r c m e r

p E A i t I v t 1 2 C e p

C P

y 6 - r r l e S I - h y n d n D e 4 l s s s p s e m i t 1 , e e y C , ,

N 1 r i c r , i

4 t c 2 e A t

u u i u d i

a u T t d o m c

u F 2 e n t

c c i I I s l o T e A u g

a J T a T T l p p p e r n o -

M a t e e s b i 7

g C u f r e a 2 e p n s S e n s e e e P M a G G p

s p p e L

r r i l , 1 u i G n t e o n I t r r r r P c n

g D e

o f t t g t i B B c , o f f f F g r r o o t a

g a e

a a a A

c r 1 f e e - e T t 4 1 4 1 2 e C c r r a , l

l e m m m e o i

N c s n

l - 2 , , k

, , n F c l t t e

3 c

e p

b e u n y y n

F A e D i i i 2 a F M e

L e l l l d p t p p u p b

t y y y a

a 6 o d I

, i i P l l e

I b g s a F R e e

l t u

l n e r I i t r h F

o m m m

n b u i t P e e r n s

n I c l t B y e 2 1 o r 7 o C ,

i u c n - t , e e i e t 4

g t i

s , , n 1 i n a

e g r

T G n

m m m

p B a i C e f e 2

, c o p e

1 R

s R l 3 n c 1 b b b i p n R

t n c d 4 i o I , t l ,

e e e 2 h

e I o

B 2

n r , r r r B

a ,

n ,

8 7 5 ,

, F , , ,

e B B T T k M a c B B T e A B r M C M M T L L A M H e M d a s M f E V M B P M n M t o h e i e u - - - - n n n x e e e n o a - - c - c r - l l - - e e l y o o a a a a a a o o c

c g a l c c c y c l c c l p b e d d u u n d n a a r t t d i l m l r e e s n t t t t t c n n c i i u t s i e e e t l e e e r s v v o r u u u u u o k k d

- a n n u e h o t r u l l e a R , o o o

l h l l l l e a a l l

l l o o a i a

o r r r r r t r b c l l l d l d l l r s s t d T l l s s s a s e T l s c h c c c e e e e e i e o e t a s s t t h c c e t t t p l

, , s , , , e e ,

- s t e r o i t y - y y e B B o

m c

c p y y e r ,

i s c l

e h c m g B B B t B

v m n n m i l

( d d t t t

r l h y o a p

t t - - g , r e d e e e i e d b e y l a r a a c e e a a a

- - - - c c

e i t y . e a n n m o T T o o s - s s l c c c c x e r

l a u e g e t t s s l e e l t t l T c S m s d a e

o n d s , , , u u e e e e - - p n n i n l s e l c . . t

l l o u e

- l

, , u n l l d t p u p

p g

r r l l l l r s o o a a d c ,

s e o

s e l l l l e n n r a a t e

t s s s s s p l

o l l l r

u c c e n n B , s h r e i , l l c o a a B r p a a o o a u

l l l s . , ,

i l i n

s y y o a l l d d t

a a

y y L l e c t t - i k k n l n s o - c i f a r b t l

e t e s , t t c b c t r m o c

n s c t s a y y

h t i i

e e i d y e u B B c s e s c l l p , i l l o f e

e t e l l

l t c . d e t e n

e i c s s t e e l i

t e . s l t e o l b y a e - - n i v l l e o p r

i e

h l

t t g c l e , , l a v c c l , r r

c t g s s c m s i s r t n a

m s

c l s t a

y u e g m l o a e e e l a s T e ,

s y c c , ,

l e . d t , y .

m a s

c l y r e r b t s l l t m -

l t e e m a B g l l v m

t e a l h e a

c c e

e c n s s e s o e d l , n a l l r o r e a - d e

c a l l l n e e s l , , o d a .

a l s s e l s

e a c l e n

o c

d i l s r s T

l , n u l l m s g s n

H ,

n , l t e l m o p c

u l d y m c i s

a e t .

s s - p o s d l s

o o e s m u r

o l p c t i o t i

. o e

n n . l g

o l e a m r t T

d ( c o p l a e c c h c a n a s o h n l o u d d a h p s c - y l e n i e y s l e a u n e

t n s p c o c e l p

r s , r

h t m h s n t o m m l i

l l e o g b e

y d ) h d c l e t t l e r l B a

t s e s

d i

i c e s p a t e h s l y o c

i c a a s v

a i l , e g l - . l k y c r p

g , s

e t i

h n s y

e s , c e n l s

o a

d e c s e e L t r

n r u , m t t a e d e p l o c u l a c d l e ,

e s l s

s r o s o e

s e

f l h g s

l l a g e b y c a n , e o , l , n l a

o g w g l

f o . e e l t e s t l l s a t s g d a n l e s d t c o e i e i l l e e t

a e n m n

s e i l s u n r

, c r l d n y r c l a r s l e d t

n s a l o l , o i l t m

b d t h n t

e u o d t

p . a n i s a i v e

c i n e i p c c v s

c a o r a t l t t o l . t d n e i e m a s s a o h y u o h o a o n u e

n n

t t

c l , l

d r u t s m e h l s T i t p

a l r t

a l y d e o l

s e o e s p a s m a s r d i

l e - o l e n n i s c . m a b

f l c , a r r m c

e c l l a

l . y d o e s a a i

l e o e e

o e l a t

e d n n n o

t i ,

k p

n a t c x l c v i o e

l e B c n l d i d e a d

n a i

h c c y p e d a l c s s e s n t l r

- n t

r e o t i r r l p e t i u n i ,

c u l a l d e l o t n e i e

s c t l d s r o i c s r l e d r a i b n c o s l p

o v ,

s o a , a s r n c ,

y B l e t

s . d t a

l h f i p s u l g p c

l s e h e )

o t y t

n e a -

i t a , h k i e e r e , i s o c l e m e a l

c d t n

a l l l a i g i s l s s n o i e l n l

l l d r l

c r l a d l i s s p c e

l c . e

a . i m i l

u t t l , r

i t

h s s o e i r l

i y c l

a . . c o r l c

l a s

r e c s e

. t

l y . l e

l l

s s t d e .

m i S C a B R F n B p R R S b a I m C L F R L C E p R R S a C R B S C L a n n s r a d o a o a n e d u o - i - i x i - c e e e o e e o e e o e e h t g o g a e g c c c

e c h c w r t d c h n r o a g g l s g c s c g s g l w i h n d m n t d e e m e l b n l t t t r e

c p v r t e t e t

u u e o i u u o p a a a u a i e l l i i l a i n i a i a t s e s m l l m m l t a r s a p p l l l x r c

i p h c l l d s l c l l o a a a d

f o a a a l i i i

a a n l

l a t t t f y o o a

t t t

t h a h t u u u o t t o t t i i o t h n a c d s r d r a a i t t g h n l e e i e c n n n o l r a a y g

o e i e l l l

r r t

s s h h p a a r e n a c e e o i a t p a s s i w

n g s t n n o n s s i v

f f

e e e y o t t s v d t r

d n h n s o o l t i , i i i i s s

c a b l

c i y

o h n o o o

i r s y s t p t i

o B e r a i s t r r T

r d d y h o l e a m t h e l e r y e o d n n

i m e

n i n e t i o f . o g o d - I c t o e u u -

o l m

n t

e c L

l . h f c C c B a p H g a n o o

n E p o o o

u p n c c c

s e a

2 P e s k e B e i

h y n a (

m c - . D h f f t f t i h I

d t p c 2 p a c

i s v e

. i

c i p V l a

l l l o s d y o o t P T p B - B a a o • • • • • • • • • • • • e o y s t l

4 t t

n e t i p m t c n

. c t i i

c o (

n n a - e o g a t r y c v d . f

- o - o p 9 a d d l l c e e d h

t o y

c l r e . .

r s y i r o c a C H

s o a e a a d

e L

i l t

f a e t R R m p s

t n t c l h t e f p u i e n v

a e p e o l s . y

i - o i l m h i i l s r o y - p n l

r n s n e g m e c s d e e . e

e l d e B t r o o l t t k p g n

r t . r c e

c n g

l y o s n e r n g f o s

a I s V l T a o e g o e r r , e t I i e g o l a u e i s

f d

o k o i f n u e f o - r y e n o p L p

2 e l a r

f p E l l l i m c r c i i C i l w d e i r

a o s c n

n n n i f n r A c

c t m t t f r f e o , c v e o . a r

u n o t i a t . e o ’ D

e k t t g a . d o e s a -

i l l m e i h i

r l A t c t r l c i e r v n 1 r i l s I i

i g i s n n e 1 f a

f u a l n . o o

a c e a d e a

f n l e

) p c

1 a

r s g o s

t i s s D t d n e n . l T s c i d

n t i e r i i l t o s e

n . a g d n e

o r y

t o a P u

l ,

t . - • • • • • • • • • • • e t a i ( c

l e e T s

i n l • • • • • • • • • • • • • • • • • • • • • • • • • • f

c - l e u d

d s n c v E t a n l c y f l f v o e n c t a i m - s e e e e o . g c

a o

i e c c c e B

, f c m p d a s s . u n • r l a t

o u r m t n o s l e t i

l c e e i t

i n

e V s o o k t c i n o n r n o t u p

s l s e v l n r

a p p d l

i o n p l ) e n . d

d C r p l a r a b s

l a

u t a n h l

o C l c . m l g L . i e

h c t n n

l a D l t s w c a f h d d a y • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • l e s y l i m e 3 r i i s d y t e ) t o f n t d a o

g 1 i

m i i

i e . e l v b a

o t c f r v t n l g e n h t o m h o 9

f o o r a

o d a n d p t e o t a a i n e t i

c ,

n s r l

n h p h a t

e a h r e t l t n a y t s i l C a c i h i o y

e h e r e n p a n o o t i

l c d f n o u o i s i o i D f n n r s e p i t d b d c n o n i

d d

t h l i c n s

C i a C s t e

y i i 2

. o m a

. d b t

. k i c d i . e .

r t s i i

s a • • • • • • 1 4 n n D s n M e

i H

l • • • • • • • • • • • • • • • • o i p n a

o r

m t

b . f o d t

a a 3 i n o h a f g g r n a a • • • • • • • • • • • • • • • • • • • • • • • • • • o . t

e n f i l n

0 y n s e e s

p g a n y t y M n a r d s p d + u

3 r l d

e a

p p m c . .

a h e

e r

e n

5 m

n C l

r • • • • • • • • v M r l e t y e

d a c x o t o 0 a i s i t s o n e D m v r m p i y i s e l / r w a i

i a n 2 d d a t

f r C a s 8 d p i t a e a t o i . c 2 o i l e

n t 1 a

, i i 3 h

e r c a a t P

o 0 t w s r s a i . t o h D o h n o n t l o .

n • • • • • • • •• • • • • • • • • r i

t e

a e o p m i o d r l t i r d e n e o . e o s

d l n

o m o i

r x g

n a

r a l a • • • • • • • • • • • • • h e . i e t i l n • • • • • • • • • • • • t .

s e h o l e

n e l r o

• • • • • • • • • • • • • • • • • • • • • e

. i u e a t s • • • • • • • • • d • n s f

e a

k l c i

i s s o • • • • • • • • • • • • • • • • i s u t i e C n h

n e m o b l - e 3 l

. 2 r u y .

b • • • • • • • • • •

• • • • • •

• • • • • • • • • • • • • • • • • • • • • • l

• • • • • • • • • • • • • • • • •

a • • • • • • • • • • • • • • • • • • • • • • • r

Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin

Protein

Immunoassay kits P h ro Rat monoclonal [M1/70] to CD11b Mouse monoclonal [4H11(APG)] to ig d h u (ab24874) CD34, prediluted (ab18228) lig c h t ts Species reactivity Conjugate Species Reactivity Conjugate Mouse FITC Human PE Human (predicted) ab18228 staining CD34 in KG-1a cell line by ab24874 at 1µg/10 6 cells staining BALB/c flow cytometry. The Blue peak represents splenocytes. Cells were then double stained with a negative control while red peak shows signal rat monoclonal antibody to Ly6g (Phycoerythrin) with ab18228. (ab24884). Lymphocytes were gated and analyzed by flow cytometry.

Mouse monoclonal [MEM-15] to CD14 (ab28061)

Species reactivity Conjugate Human FITC ab28061 staining CD14 in peripheral blood Mouse monoclonal [CB19] to CD19 mononuclear cells by flow cytometry. The cells were prepared using 10 µL FITC antiCD14 to (ab1168) 50 µL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated Species Reactivity Conjugate on viable cells. Human, Chimpanzee, PE Rhesus Macaque Mouse monoclonal [SN3] to CD24 Whole PBMC cells from Rhesus monkey were (ab77219) fixed with paraformaldehyde. The lymphocyte population was gated based on forward and side Species Reactivity Conjugate scatter properties. The cells within the gate were then analysed on 2 color dot plots with the CD19 Human PE (clone CB19) plotted on one axis and other lineage makers plotted on the other axis. CD3 ab77219 staining human MCF7 cells by flow and NKG2a positive populations were negative cytometry. The primary antibody was diluted 1/5 for CD19 staining. All CD20 positive cells also and incubated with sample for 30 minutes at stained positive for CD19. Additional analysis 4°C. The purple profile shows negative control was carried out to verify that the monocyte and (mouse IgG) vs. green profile ab77219 (CD24). granulocyte populations were negative for staining with this CD19 antibody as expected.

Discover more at abcam.com/CD Key : Featured product C C C C C C C C C C C C C C n T C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D D D r m g 4 5 4 4 4 4 4 4 4 4 4 4 4 5 4 4 4 4 4 4 5 5 5 4 5 4 4 e e 5 5 6 4 5 7 5 2 2 2 5 1 5 3 2 3 9 9 8 9 0 2 1 9 4 9 9 t R R d b a R R c c a e f b d

B A C O

------

L I E f T P M E L G I T B I P P P C n A I L P I I I I C I I I I I I n T T n n n n C n n C n n T a e e a e e l L C T p l l l L l t o a P a t t t t t t t t G G c a a t a e G e A A t e e e e e e e e u m u e u A P i m X t r m M t t t t g 1 m e c a g g g g g g g g A A M M k k A e e r e o k S r R e , n B l a p o o s r r r r r r r r e i l l l R p

2 2 o 4 r n b e e e i i i i i i i i 3 1

a T M n p C B a t n n n n n n n n c s , l

c -

t t t

r a t e g 1 y i a t D

, I

i a C a y h g g a a g a a a a a a

, v l l m t , p H n y I

e l A

n t - e l l s l l l l l l l l

G i P B h c y y p y p p p p p p i n e 1 U

n s e

e F o a c c c s h h h h h h h P ,

C , o -

p T

o o o i -

a a a a a a a 2 G H a 2 3 c c M o t r p p p C - - -

- - - 5 o l ,

B i o E c o a I 1 5 6 2 4

a d m r r r V 1 I , t H o o o h e b g l

f , , , , , 5 t e a M

L e a

t t t a m i - , V V V V V l n

c e e e y c

A I d c i G O ,

L L L n L L c t a i i i

I o -

n n n o t b L p 3 o A A A

A A y P o X n

V g

H r V I I p a

s - - - - - r a X b 4 o

, l a 1 5 6 2 4 y l r a ,

R p l

I t o c p , p c , , ,

b G T b e n

s s , t c G o

h I I I r u e i

e G a t u u T T T n P P o e i p a n t

P i g b b G G , G a n c A l 5 G i t r

t e u u h 9 I e M o e

, V I a A A A c

r P a b n n n t S i

, l a h e E n p 1 5 6

i , i i - n , M P t t

t a i

h 1 R

, n , L i

a a

i N n a , S G n ,

- l l

, p p g C

K P I

T h h

A 8 1

a a G B ,

, ,

e H E l m m B c B p a m d T t B M U p B m e C A m H P P L P P L P L L A T M T P M E m P B n n y h y - n n e x r e e e e o a l h h c p - - - - c l l l l l l n l - m e b e e y a e o e o e o e e c a e a a a a a a a t c

p d d n u u u u n t t t y y d a o a a i m l m m i i e u d c l n s s m g i s t t t c t t t t s e e l p t v q a r v m m d o k k k k - u e e e e e e e o n t n n h r e t l e u d r u o a m h t a l l r h l a a o n

o o o o a u o r t l l l l l l l l l l o a

t d d d o a s s a e e e e e e e c e s r e h o o l c k i e t s l h o t t c l o c c c c t t p e s r l i y a a

, , p e h o s d r i t t t t t t t y

a l n e o c c u t m y e T T n

c y c y y y y s s s s s s s m k i o

h d o m

n e i r d h t y p h a y y r d l

b o p

t y c t t t t l - - a

, , e i y i r

B a y i a c e l e e e e a m a n a a a a a

c c

o t t o T e l a a r s l s t n o a B

ï o T p e e s

e o g e e i t s s s s s e e t v l - t d n n n n n b i - c s s . t l

o h , e u c a -

c s s m i c r i h c c - e

l c . s

. , , e

l l o d d d d d c c i l e c e

l t

c (

l l p t m o y a e , , t e r l s e y e s s s c e ,

d t o s i e

i e t

a t l e p v h c T m c o T c l n

s l l t h m m m m m l , , , e l x u i

l y e l l x h a l a l e l

l s l s a c a l s

y e e l s l - t l i -

s e g t B l l c s m p c

e c n e s o c c k n s c s c l , e h e e e e e

, t m

s l l s ,

l r a ,

e e c e l l a e r . t - e d m e i

, r , e m i c d s m y T

g g g g g u a d l p i , a e n

c o s m

l n l c d e p n o p

m y e l r l l . m e

l - a a a a a b n l

l m h l e l g a s o i u n p e

c s

l a e d t d c t t t p r c l s o k k k k k T c l s u e

, i o l u o t n

i b a h n o e ,

c s y l r t p

a n u

e i e u a a a a a

c s e e n - l e s m t e c o a l o n s t a m c

t o l t

a h c i s e r r l l e , u b c e r r r r r l t t o ( , y

p s l s c l e o p y

l a g y h s c

y y y y y e s p t n t e e s l s m e l r s s o m t c

y t e , i

t l t c l , y o o o o o o e a t e . e t l c i

. l c g r e

o s e l ,

l n y

t n h i h t a

d k l y s t

m o a o e L c c c c c s e l s l , o h i s L e . a s e L t

t t i g o

e e n i t

r , a s k e y y y y y , s n l

o C l l , t n y o e o ,

l k

s a

l l o ( e e

c l t e

d e l , e s i , c t t t t t l g s o i o B n m w o m l w s n

i a s

e e e e e a D n m y r l c c c i m ,

e n a m r , r e c s o , c n

e y

s s s s s l

g a

y e o d a

o 8

t l d n h y c e e c y r t e o a y t l c c e p t . . . . . s o h e e s n

t l c e c o n + e o d e t h e x s t l c x p

r c e e l e i e . n s , a o e r y u a t t y n c o l p t

l m

e r p

t l h h i l l l s l T . h m

s o e a t t b i v s n l s

o y l d p c r v s e o l

r B s

a e s , . - e l c n a a

e ) s l o a e t y s

r a c l , o a c a

s c u . e n l a m . - y

l i m d t t s m i

r s t . i t e t e y ,

y n

a s i o n b L a s e s t t i e e o

a s o s c e e a t n t l t m o l a p d . s a o

l s l i e

s d n n e i t

c s o l s c i c o d c e h c

, w e

n s

. o d s c d c e ,

n e a r T n y o e e t

e i e t a , o e e e

s C

e l s

l n t -

l T c t e l l a l o l n c o s e l l l p o

h i l t c o s n , l s s d a l D a i - l x n

s s o s , d n c s e h n . o e e c c d ,

l p

n i

) a d e r 4

o a l n a

y e l a s o r c , a m t l r i r B d s n

n i a + i

s n e t o g l e t r e e s c m n p e l

i , - l d d

l t e

d s c p m e s t a

l s e

d T s l l e e c c

o a o t d

s a s

h s l u e e o

i - , c

a e e o p o n n n t

u i c , t t i

b r o e h p n a c l

h n e l g b i g s n o d e y l g l t s l l d n l e l s n y h d l a a l a l a ,

t c

l t r a s e e

l h

o T

l d m e a a

k s b r i t a y i . s a t s a t n e t y r -

l a c n t s s t a h n o i c t o u s c l e

l a s

, r t u e e p ) s d e b

. c c t b

s . l , i l t o w

l l

v l e y

o i s o a , s c l a a

s p a t l e c , e c i t e l s l

. t e

s h t y t

y l c h t e s

l s , l i i

s t t e e

c l

e , e s ,

s , s s s

, . . .

e e C R t p a b L F C t C C f C m C A P P P P P A C i S A G A A E A A E A A n h h e p n c r u a e d l l l l l n i d d d m d d x d d r e o r r r r e

l y a r a a a a a g y o y c i i i i t i i d s u n t h t h h h h h h h t t t t t g l i m - t c t t t t t t n r m l c i i i i i v a b t i f i

e e e e e - c c c c e c h a o k e e e e e e e e l e u r c a a o a a a r r i i a a a a o t l l l l l m v o s s s s s s s s z e c c r t i y e e e e e i a d l c s l t d c o y h

l l l l a

a i i i i i i i i i a

o t l c

t t t t t t o a y o o o o o o o o l h t s h i e i i

f f f f n

e t s t n o a g v s o o o o y a a a a a l f n a n n n n n n n n n e e e e i p n s a l o g e o l e r e r r r r t d d d c d

n i d . ,

, l s s s

h t

e e o

a a a a a

r c

n t l n A B B B B h h h h f i i r

c s

i b

a i e o o o

c o m

f l d n n n n n o e c v e e B e e e e e d r .

- - - - n a c s t n n

l c n m e d d d d d a g o o r

l s e i l S s s s s u e r a a a a - o l d p l t

e . n

t u

i m l

u r f i i i i a o i

c a c e s s p i s m m i a n n n n n

o o o o l h a o o a B l i d l g o i k n t f o i i e l a , r m l d n d d d d . n n n n a n g g i

p a n c s

n r k i i r o n o b l d r a s t n g i e

t h i g n n d t t

, i e t l

b . e T T T T a a a a n i c o t a

e r a

d i e t o d r d u i a a T c e s ,

r h a r a y n - - - - m n n n n . n l

l n h T m s

y r y k

c c c c e l l -

h s t • • • • • • • • • • • • • t o n r t t

c d d d d t

c d

e r l e o - o i i e e e e t t u e t 1 o i r i C o o n f e r r e c o e n

e o

s s

a c l l l l a a a a a a a l s

f a n g s l l l l d m a l n e s D o p m

l i

. n l y n l h g g g g n n o g c s r r r r g e ,

t l , s t r t e e e e

c c 4 i

l e s i g g g g s s n g o

e g a h l o v o (

i a n t f a a o c c c c t 5 r d d c l d o r r r r

l s r l o n e o l n t a w d e g e e e e c e e e e e r

s e

u u e

e u c s m . l b l t i n f d c g g g g t g o t

p p p p

• • • • • • • • • g o n h c c l r o a u i ( e a i c o l m d e o a a a a a p t t t t v

G i a

t t h i - g r o o o o n m l l n t c h m i i c

p m t t t t t m y v l o o a i t i u c i i i i i a s h P r r r r g b o y i d o o o o o e i t o n n t t

a e e v l i ,

e m m m m d i i i o I a n

n n n n n l n t

b b a n . . c o i a ) d a l l

a n r l n t r

. . . . . • • • • • • • • • • • • • • • • • • • • • • • • • • • • • . p s i l n

e e e e a l n i i a g a

• • • • • • • • • • • • • n o

n m t c

a o A t R • • • • • • • • • • • • • • • • • • • • t m h d . a d d d d t n n . a d o g

l d t n e • • • • • • • • • •

i m a

i e n e i i i i e c L i n s d g d a a a a r a e . .

n g a d

c t h e • • • • • • • • •

i r a p t t t t c

a d h t v g i o e e e e a c e v

o g o p t n l a d i e i e i c d d d d a t e p a a r f

v a i p g p h i S e s o y

n l e t g a t t e a a a a l o r o e

s e t d n i I - s d p t e o o x o R c c c c r m p e s i d

o s a p

t t t t t p n i t l f o o P i i i i o

• • • • • • r s n o o i a v v v v n l r a l s . a f a r a . n

i . s

r a a a a t t

c a . a

A c

i r • • • • • • • R t r C .

i p t t t t g e e s R t t l i

i i i i e • • • • • • • • • p d e x i o o o o o r e e c 3 . l v l e e o h

) n n n n h h r l g n • • • • • • • • • • • • • • b c a

u t g i a t . . . . t o e

o t

e o r w • • • • • • • • • • • • • • • • • • • • • u l a

A i v s l a e n i m c o a n

l n h i l a i h r s r t s t t o n h d

u i a p e i t p y o c m v e i n . s t

s b l o

h a

C e e i s A r

a o i n

o 1 l T e t m

m u 4 o c l t m s n . - q f s r b h r r c

e e a • • • • • • • • • • • • • • • • • •

i u l

o o o o x e e . e r

n y

i t e

n r . l f l S m u o r t

e e

I e a c

k a e a m c d o e t l q t e h a o c c t u

e t p s f p u a l r t y t c o l l

t i o i

v s e i x

o o i v

y r n a r m a i .

• • • • • • • • •

r a r e s t i

• • • • • • • • • • • • • •

t t e n r t o

b d i a o i a o s

o n i

n n l n n • • • • • • • • • f

n t o t . d . • • • • • •

. • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • r i

• • • • • • • • • • • •

• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • •

Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin

Protein

Immunoassay kits CD56 NCAM1 Neural tissues, natural killer cells, T-cell subsets, small cell Homophilic and heterophilic adhesion. • • • • • • • • • • • lung carcinoma. CD57 Human natural killer 1, HNK1, LEU7 Natural killer cell subsets, T-cell subsets and monocytes. Adhesion molecule. • • • • • • CD58 LFA3 Leukocytes, erythrocytes, epithelial cells, endothelial cells, APC-T-cell adhesion. Killer target cell interaction. Costimulation of CD58+ • • • • • • • • fibroblasts, hematopoietic and non-hematopoietic cells. and CD2+ T-cells. CD59 Protectin, MAC-inhibitory protein Hematopoietic and non-hematopoietic cells. Prevents complement polymerization. Protects cells from complement • • • • • • • • mediated lysis. CD60a CDw60, GD3 Subsets of CD4+ and CD8+ peripheral T-cells, platelets and The CD60 family are oligosaccharides present on gangliosides and are •* monocytes. involved in co-stimulation of T-cells. CD60b UM4D4, 9-O-acetyl-GD3 Subsets of CD4+ and CD8+ peripheral T-cells, platelets and The CD60 family are oligosaccharides present on gangliosides and are •* monocytes. involved in co-stimulation of T-cells. CD60c 7-O-acetyl-GD3 Subsets of CD4+ and CD8+ peripheral T-cells, platelets and The CD60 family are oligosaccharides present on gangliosides and are •* monocytes. involved in co-stimulation of T-cells. CD61 Integrin beta-3, ITGB3, GP3A With CD41 on platelets, with CD51 on macrophages, endothelial Mediates cell adhesion to diverse matrix proteins. • • • • • cells, fibroblasts, megakaryocytes, osteoclasts, mast cells and macrophages. CD62E E-selectin, SELE, ELAM1 Activated endothelial cells. Leukocyte rolling and adhesion to activated endothelium at inflammatory • • • • • • • • • sites. Tumor cell adhesion and angiogenesis. CD62L L-selectin, SELL, LAM1 B- and T-cell subsets, monocytes, granulocytes, natural Leukocyte rolling and homing on activated endothelium. • • • • • • • • • • • killer cells and thymocytes. CD62P P-selectin, SELP, LECAM3 Activated platelets, endothelial cells and megakaryocytes. Leukocyte tethering and rolling on the surface of activated endothelial cells. • • • • • • • • • • CD63 LAMP-3, Granulophysin, MLA1 Activated platelets, monocytes, macrophages, granulocytes and Translocated to cell surface on activation of platelets, endothelial cells and • • • • • • • • • • • endothelial cells. granulocytes. Associated with the early stages of melanoma tumor progression (in regulation of motility and adhesion of melanoma cells). CD64 IgG Fc receptor I, FCGR1A Monocytes and macrophages. Binds to the Fc region of IgG with high affinity. • • • • • • • CD65 VIM2 Granulocytes and myeloid cells. Low expression on monocytes. Adhesive carbohydrate. CD66a CEACAM1, Biliary glycoprotein 1 Mature granulocytes, epithelial cells, hematopoietic tissues, Neutrophil activation, homophilic and heterophilic adhesion. • • • • neutrophils, colon and liver. CD66b CEACAM8, CD67, CGM6 Granulocytes. Cell adhesion and neutrophil activation. • • • • CD66c CEACAM6, NCA Neutrophils, epithelial cells and colon carcinoma. Cell adhesion and neutrophil activation. • • • CD66d CEACAM3, CGM1 Neutrophils. Cell adhesion and neutrophil activation. • • CD66e CEACAM5, CEA Adult colon epithelial cells and colon carcinoma. Homophilic and heterophilic adhesion. May play a role in the metastasis of • • • • • • cancer cells. CD66f Pregnancy-specific Epithelial cells, placental syncytiotrophoblasts and fetal liver. Immune regulation and protection of fetus from maternal immune system. • • beta-1-glycoprotein 1, PSG1, PSBG1 CD67 See CD66b - - • • CD68 Macrosialin, KP1 Monocytes, macrophages, neutrophils, basophils, dendritic cells Possibly has a role in phagocytic activities of tissue macrophages, both in • • • • • and myeloid progenitors. intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. May allow macrophages to crawl over selectin bearing substrates or other cells. CD69 Activation inducer molecule, AIM, Activated leukocytes, natural killer cells, thymocyte subsets, Signal transmitting receptor. Involved in early events of T-cell, natural killer • • • • • • • • • • CLEC2C, EA1 platelets, Langerhans cells and activated macrophages. cell, monocyte and platelet activation. CD70 CD27 ligand, CD27L Activated B- and T-cells and macrophages. Induces proliferation of costimulated T-cells. Enhances the generation of • • • • • • cytotoxic T-cells and contributes to T-cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells and immunoglobulin synthesis. CD71 Transferrin receptor protein 1, TFRC Proliferating cells, reticulocytes, erythroid precursors and Controls iron uptake during cell proliferation. • • • • • • • • • • • activated leukocytes. CD72 Lyb-2, Ly-19, Ly-32 B-cells (but not plasma B-cells), macrophages, follicular dendritic B-cell activation and proliferation. • • • • • cells, epithelial cells and endothelial cells. CD73 5’-nucleotidase, NT5E B- and T-cell subsets, dendritic reticulum cells, epithelial cells Nucleotidase. Dephosphorylates nucleotides to permit uptake of nucleosides. • • • • • • • and endothelial cells. CD74 LN2, Ii, DHLAG B-cells, activated T-cells, macrophages, Langerhans cells, Intracellular sorting of MHC class II molecules. • • • • • • dendritic cells, endothelial cells and epithelial cells. CD75 ST6GAL1, Sialyltransferase 1 B- and T-cell subsets and erythrocytes. Catalyzes the transfer of sialic acid from CMP sialic acid to • • • galactose-containing substrates. Cell adhesion.

Discover more at abcam.com/CD Key *: against pan-CD60 : Featured product C C C C n T C C C C C C C C C C C C C C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D D D D D D r m g 8 7 7 8 8 8 8 8 8 8 7 8 8 8 7 8 8 8 8 8 8 9 9 8 9 9 8 9 9 8 e e 1 9 9 5 5 5 5 5 5 2 5 4 5 0 7 5 5 5 5 6 3 5 2 8 4 3 9 1 0 7 t b a i h g f e c s b a d j k

- -

A T L L K B M n L L L L A S L B G C L L L H L A C C K C F L T U A I I a I I I I I I I I I R C H P A 2 l L 7 p L D B T 5 1 P l L L L L L L L L L L L B t o m e P 9 A - o R R q P A L A T I Y R R R R R R R R R R R 1 2 1 1 b 1 r , p A R 1 M L e 5 D 1 R n 8 R 1 1

, A A A A A B A o B B B B ,

, 1 , ,

a G L , , I o t T 1

, 5

1 5 2 4 3 5 6 3 2 1 4

G r S A

T , C C t G s e i , P

, , , , , , , , , , , B i a

N A L i 2 t K 5 1 L I I I I L I I I I I s 2 L r o e e

L L L L L L L L L C M a

F A Q I I P , A s t T T T T T T T T T a

R R

r s 4 S B y R R 4

U a n R 1 1 7 6 8 5 4 2 3 p 6 8 4 l F 7 3 1 s t c , , , , , , , R 1 a

g p - , e A 6 L L L L L L

, n

2 ,

1 e L a r I I I I I I

P M i R R R R R R a M n

n I n

R O m - X 7 4 3 2 1 5

i O n 2 l 9 i E , R g i

7 d 3 M 2 R

a A e

n I

4 R ,

, 1

F L 0 A A

L G ,

f a T c n e B e d k p L d i B B C B M E N M A L H B B M G M M B A M M B G M M M N M H G n i b e i - x o e l a e c a e o - - - - c a - - c - l e a e a e a e r r o a o o o o o o o o o c l

c c

c c c B r p t l e a s n n n u t a t t s s a a l l t m t m i t o r e u n t n n n n n n n n n i i s e e e e e l v u u e r i v v r i u m n d g d k n u u o n n n n b l e r , o o o o o o o o o o

a l l l l l r r a l a l a a o

r u r e r u c l l l l l l r a p

e d d o o a a s s s s a s l f s i c c c c c c c c c c e i i k t s a t t t t n c e i t t l r l l e h t p

p b . . , , e , e o o s l o l o r i i

y y y y y y y y y y i u

h s T T

c c s y t k a l

B d i

k k h n m m i h l r d d t p t t t t t t t t t p t c c l b

a o

t t - - i e e e e e e e e e e ’ s o l a i i

c c s l e s i a

- y i y c c

o o l l l l , s e n n l c , o o B B s s s s s s s s s s s l l c b e s

e e

t s t e e e e

t i i e b a l n s e e e m ,

, , , , , e , , , , e u e n s l r

y - - l l d

l l r r a a l l

a n ( l l n

d n m m m m a m g n m s s t t t r l

s s

o m

s c s c a a

l u

n i i y c c s a n s s s e d t c , c , o T t c r e , c , e . e h

n n e r p e a e t a a a a a a d a u

l p , d

m m u

t t - e s u

e y n l

l c c e y i d d h l r l k n l c c c c c c

c l n l T b h v

p t o l

l s t s n B t

e a e e n s r s m a n o o i e

e r r r r r r e r d B u a - s o l h o

i . T T d o o o o o o g o t . l

l l d a - c a n d n n s l l e

l l e l u a t c - p i o i c l - - s s o B o p p p p p p

u p e e l a s

t n c

d o o o c c r s t y r n c u e h a , c , c t h h h h h h s , l h

d l l a - e

d e e

t c c , a h l t

c e d i y r l n n c n h h

. e c m l a a a a a a l i

l l a y y

s l s l e

s t l e e

l l B t

e l l a e t B d s e e e e s l s k g g g g g g e T s s t t , u e l s . o l l o

e e l , t u

l p i

l . l d i , , - e e e e e e l s p - k

s a

u i b s l d l a s

n c a s l l c s s a r a a

m c . y e i s s s s s s l , , l r s i e . n o a l o e . e , o ,

n n

l a i t n m , , , , , ,

p c e e e r

n o s d n n

c a l d n l c

o d c l T l d m B d g n l e e c n r t

m s

d c e

p y c e a s p k u

s m -

a e r e l e r d - . c p y r T l l l t r y c h a n , t i i h u l

a s l

e i c n u l o l

u p a

e s o t a l c t l e - e l a m o n d a

e e i c b s i t s d r c p . r h l t a l c e t n i l l c s l c u o i y r s a h , s v r s o l , e s n r h

o e

l i d

y t c m l t i l n . a a m e l e n i c . a l o s o t o g i

a n r d d

l t c i e

k a t s n t l a T e a c , e n i p c

i g a i o s

o d

i a l d o

l c d f

l - l f c l t l s y d h

a . e

l c c i l c

s n i u c l c s e n b e e b d b

a y y n e s . n e o a e c r e , B n ) r r l l e r l

a t l t o g

d l

l o

a e l o c n s s B l o e s l a d a n l - c l s l o s e

s b y , t c l , b

i s n e r e - d n l

, k d a , s d s

t e i n

l s

t a d a e l r n d a a i r e f

d n

l . i

l o i l e i a s d m a c l

t m l n

s s d b

n s n e e s i d H d p m u , c

l e n t , d t o r

c d . r a n

e o s

l T k t o i n

a d t a c

g t e e r

o . c c d h , f i n n h T - b o d e s

t i h l

c e t r l n b r s y e l d o e T - e h l p t l r o s i a e

l c l r t m t e r i l s r c e l - l r e h r

t i o e p o i s e s l i c c

a i a o

u y l t x c c l i a o c s t h , b i t

e l i l n s c l t m

t l a s p s

c e

y , n e n s c r a l c l c

d a . , o e l l r l t e c d

. y s s e

g a e l e e e

c B

s p l e t

n ,

s l u e l l s l e s m

a e t

l h l - l s n d u s c s t s s l . , s

n l r s i .

l a . o e a o b . , l i o s ,

s d o .

n n l t t s , p

l . u h

s o d e l h

u r e

t a a

i n s l l

y i s l n a , g

, e d

p r v a k a g S S C F M M p a m S M H C M M I I I R S M R R p r d R I G C I I I M L C m C n n n n g n n o e a i u e r n r n h i a u u u i i l D o o e e e e o o e f r d v h t A h d a a e a a a a a a o l g o g s l f r e e i a t n d d a r n b b e m s c c g c s s l n o i p o u i i u y y d y y y y y c n d d n

g 7 l o b b

r n e

g F

c t e e u u e t t r u g l i

r o i i c s c m c i a u a u e 5 i i i v n u m n i i o e a p a a a a a m t u r o o m m m c t t p p n n p l

e u e h i n a l c l c

e s o i a

o o s v p n c l c c c c t

l p c o e

s t t i i t i a m e s p o s t t e e t t s r

u u u t t t n d r o o t o

o t t t t t f n h t r r y i i c d h n d

e e e l y

o o i d e e c m d a a o o i l l l t

r r r a a a a a a a l a M t s a i u g a a a i a

i d e i c v

s l

y o n n f

r a i n n n o f f i r a t s s s s s f f p f p a f c a v n a t c t o t o e e

r t i e o o o s e H . . i i t i s

s B B f i b o

e c y o e o o o o t

n m e e n n x t

p d e r r r s r r i c r r r e r a d d a n s y n e C t

e e e e o - - p n p n

n i d e - , d a o t

s e o a d c c c c c

d u u

m ,

x a a o i . l c c c c l t t g t

n h

s t l e p t c l l e e l h e o o o o o u h d i u d c c u c u t f r a a a e e e e o s e .

u t f u u t

t l l D e

s s f t t f f l l t e e i b e p i r i i s s l l s ( o p p p p x

a a a a

i i

r n h v m n

r n a a b o o i i T T l

s v

a a l g o s s s i s s a 7 r y t t t t r s e n t e a i e r c

e l o o o o n i n n t

- i - i e

t n n

. r e . o n n m l 5 o s o y f n i a i

t c c I I I

r k • •

r r r r o d o t . . r i l • • • • • • • • • • •

d , k t t c r

i r

d . n y o

n o

e e h • • • • • • • d M M M i g e

i i i I n a p e F n

r l f f f f g g n i e e s .

l s a

o o o o n

p l l l

a h g c e e u l

l h s l a l m

M a o e e t H H H v p , e

p d r r r r . m e n e e e

r I a a x p l o n

c e d n n a t g a r

r h C C C o c c c c H p c r s o p r c c c o

S c t i o e l i

n a A t i c l i l l l l o e u l e i r r t n t t r e r i i g a a a a

y n e o C d n t o o t i i e e d c e

e a a a

p s m v v m a i g l t t e s s s s a s c n m u t l r n t c c e h

s n n n i a a

t i l

t c s s s s o e u n n m . o

e c e e e , i f r s s a t t t t t f i n u

o a n s i i i l s d n i i u t n a e f s r n i I I I I p p . g g g e t o o i e n

n l

r e .

n . o

d a n

s n M M M M P t t t • • • • • • • • • • e e e m . a

n n e c o w d g a

o o • • • • s

c s n e s t c t o E n n n r o

o l e l i

t p H H H H l r r p i e a a e i

e . t a p c a o e f m t

s s s I s i m n s

i h ( ( l o d

n n

r t e e C C C C r s f p

a . . . s m C C c

e h a

i a

n i c d d m l s p n t n T h D I n i a s e l

d a o n b n t D D s h . a a a a o

l d a o o a r t t l

I e p p

o n m l c l . v

l e a I e

n n n n l e c e 7 7 - e

t r r l g n e M m r r e w u c o R e s y e s n m y t t t t r

o o 9 9 n u o d c i i i i e i , a u b l

g g g g s t s n n H e e v

l l a a t n b t c u o u o i i i s

n r l

h p d e e e e f f n e h

e c n I c + + y e u f l C e e t c r

t g c e a e o u o n n n n T c e d a i e

h t t

r C C e b r r d

e o A

s r c

n s c l x s s s s - a a p

x r g o a u u e s i i c e D D . s . e e t t e

i . . . . s n i t t t u

l c n

i c d p

s s • • • • • • • • • • • • e i i o i i f I o c

a e c s o o n

s 7 7 e i n l o c t , p i t d t l a r o n n y i

n a s

l n n e y 9 9 g a n

v g n a

o i o t t f g

g . n a . d k . .

e b b o o o e r s o r

e t n

t w n e b e

c • • • • • • • • • • • • • • • • • • • • • • • a

H r s ) ) e l l r n f t m g k s v y a . . t

i r

d o

n n

S S i s s C a i a e a a e t t v n i c l

h o r u i o h l t . s t n c s t n i i i d e a o M s e • • • • n a g g i y n l n e

. e d s

g t p l m a a d g r n n o l i i .

e • V p a n

o g i u o • • • • • • • • •

c r

e a a d m c

c o l g

a n

e l r s t c a y n n l l e U a r i t

f e t

m t v i e e i t t n t

a z e g a g s t t r r e L m n i i i l r t o y a a t ) t

r n c n l u . s s 1 y u

d s . a m n n n a a i

d a • • • • • • n .

c 8 . r I e d a a

s s l n n

a l e r

e M

o s t c a n n o r d d p d d e l

o f y

r t d d - t r u u r

a k

u r i e h t i o l e o n o m m

o i c c y c l s c a r l • • • • • • t e n l t t

k e e e g p i y i i i m p t u n

o o n i

a s r i o a t n o p l n r a

o n n

u s f c n o e n • • • • • • • • • • • • • • • • • r .

n k . . y • • • • • • e n e e i n

s • • • • • • • • • • • • • • • • • • • • • • • • • • s i t

• • • • • • • • • • • • •

e n v l s e a

l m • • • • • • • • • • • • • • •

• • • • • • • • • • • n s

• • • • • • • • . t

• • • • • • • • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin

Protein Immunoassay kits P h ro Mouse monoclonal [F10-44-2] to CD44 (ab46793) Mouse monoclonal [143-30] to CD55 (ab25634) ig d h u lig c h t Species Reactivity Conjugate Species Reactivity Conjugate ts Human PE/Cy7 ® Human, Pig FITC

Overlay histogram showing peripheral blood lymphocytes Overlay histogram showing peripheral blood lymphocytes stained with ab46793 (red line). The cells were incubated with stained with ab25634 (red line). The cells were incubated in the antibody (ab46793, 1/100) for 30 min at 4ºC. Isotype 1x PBS / 10% normal goat serum / 0.3M glycine to block control antibody (black line) was mouse IgG2a [ICIGG2A] non-specific protein-protein interactions. The cells were then (ab91361, 2 µg/1x10 6 cells) with secondary antibody DyLight ® incubated with the antibody (ab25634, 0.5 µg/1x10 6 cells) for 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 22°C. Isotype control antibody (black line) was 30 min at 4ºC. Acquisition of >5,000 events was performed by mouse IgG1 FITC (2 µg/1x10 6 cells ) for 30 min at 22°C. gating on peripheral blood lymphocytes. Acquisition of >5,000 events was performed.

Mouse monoclonal [GA90] to CD45 Mouse monoclonal [1H3] to CD62L (ab1176) (ab52068)

Species Reactivity Conjugate Species Reactivity Conjugate Human PE Human FITC ab1176 detecting CD45 in human white blood ab52068 staining human granulocytes in whole cells by flow cytometry. Cells were fixed in blood by flow cytometry. Cells were treated formaldehyde. The primary antibody was with Optilyse C (containing 1.5 % paraformaldehyde) incubated with the sample for 1 hour at 25°C. solution and gated by electronic volume and side scatter. The primary antibody was diluted 1/10 and incubated with sample for 30 minutes at 21°C.

Mouse monoclonal [MEM-258] to Mouse monoclonal [B-S23] to CD253 CD46 (ab28068) (ab47230)

Species Reactivity Conjugate Species Reactivity Conjugate Human, Cow FITC Human PE

Overlay histogram of ab28068 detecting CD46 in ab47230 staining TRAIL in human peripheral human Jurkat T cells by flow cytometry. Cells blood monocytes by flow cytometry. were prepared to 5x10 5 in 1% FBS, 0.05% sodium azide in PBS. The primary antibody was Cells were fixed in paraformaldehyde, used neat and incubated with the sample for 15 permeabilized using 0.1% saponin/ PBS. minutes at 22°C. The shaded histogram 1 = non stimulated represents CD46 staining and the unshaded 2 = INF gamma stimulated 100U/ml ab47230 was used at a 1/10 dilution. 3 = LPS stimulated 100ng/ml histogram represents the control.

Discover more at abcam.com/CD C C C C C C C C C C n T C C C C C C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D D D D r m g 1 1 1 1 1 1 9 1 1 1 9 9 9 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 e 1 1 1 1 1 1 1 1 1 1 e 0 0 0 0 0 0 8 0 0 0 6 7 9 0 0 2 2 2 1 t 7 4 0 6 9 2 3 8 5 1 5 0 9 8 6 3 2 4 7 7 0 0 1

- -

b a b a a

I E S C S V 4 n A I I I T M L L c G T T G I N N N T L C I G n C n F L a - F a A A I P N N n e e C l P e e e S C M k t t F - I t N A S c e e C m e 1 2 d m m M M O c c c i A F F A F R t S t G g g R M r R F h , o t t t i 2

e M n l 1

i i i R M a a P P r r F e R R n n n

c K 2 g - R

i i 2

a R p p t a n n 2 1 ,

1 2 3 R , D , l 1 y e e I

i t 1

h h T l

S n

, M , , , i E p p , c c a b ,

7 v

o o

F , P P P L e e h L e C e l e W

P p r r

E M C V V V

p a 1 p i i

t S I n n h L a N I , t C , t

R R R 3 S 1

F I C 4 7

4 G A A L 0 r r

L L L 3

D A E

, S 1 1 2 I I 1 2 3 M

, R I , I

, , , R ,

F , T , ,

S I S

T M H H 2 G T 1 N N E E N R

G V V D B D F M M F A E E A 4 U R

R A A C B E

1 S S 7 4

F A D F 1 1

A B

g g e a c w e a a a e L e m A R M e C G E A L M L L A a I E A H M S A H M L S S H E T M F n e e y y n n n c n n a x r r n n o e f i c c n c c p u c u u p e e e e e e r t t b a o o y o o a a m m t p l s e r d a d d d d n w u d d t t t t t d i b b b i l s l l m m m i e i r a c n n n n i i i i t n n t s l l v v t r v v v v r o n o o g k o u

h h o t o s s s i

p p r l e a a o , a

- o o o o u u a s a i o l a a a a a a a o t

a o u t t e t n l e e b e e e e e t h h h a e s h c c h h s s

c c c c l l t t h i t t t t t t t c o o c a p l r e g l l l e p t t t d v e e e e o o o o o e s o t t t

r i i a y y y y n e e e

e a u y i i e p t c c

n h d

e o o o d i

v v i d d d d t t p p t p t l c c i c s t d l l l l a o p

t l i v l m e e e e y y o i i i y l h

d a a l f i f f a a

e a a a y y a y

t o o o p s a o

m n a c n r t t T T m e p s s s s s c e s

s s g l t t t t t s o e e l l l t

i i i l

l t p e e e

s e e t e , , , , e e e , a e l - - o n l

c c t t t l h e c c c

i o a a

g s s c i p c c e e e o l

l a d d s s l g l s m m t a e t t t e l n d a l e e e e c y y s e e n , , s t e i i i e e e s h m m m n , ,

c c n c

e r l l l

o c e m m

l l l l

a t l d e g l l l t o , l a a a n l l p i l l l o s a e y s

n n . s l l d s l l

l a s s s e t l y s

s a

a s s e n e n r e c c c n i l , e h m .

c t c . c c n p p a a .

. .

t s a l

n m s , t

, , n n t y d o a o n d r r n e y u

e e e e r e

d h h t t s . c n o o , m t a e p i t d d u u

l n r d o

d t p p a l l m l l l p , e i

e o o m a e o l

l l u i p p g c r

h d

r r s s s a d r t o c i h l e h a s n y n l c c t c a a h

s c i l h h t o l

o e

, , h e t o l o

t t s i e s c

t o c y y t a

o o i , y l l e i v e h a a a h d i m n c c c n

s e

l c n , v e e t t r t c k k n l t n n m a

o l e g g i e e s

e n y r e s a e e d l y i a e o v n l y c i i i a o a d - - t s l l a l l , t e e u s s l d n l t u s r s e t a i o h h l l p c t e l e c

e l a c e e e c

i i s l e e ,

s s e ,

d n

t c t d y

t n e e i h

n l c s

m y a r s i l d r r r t c

e l m , , s n - c

s d e r o t h s a o o

o m m e .

y a t c . c e n

e o p c c d , g m

d . e T e . l a

p e n p p

m c l c e o t e l

d s e e c l l s

s r a a u l o s - e n s a c h l d h y T h e s o l n a l . c i y

l l o

l l a , t t r a t

l l p d p t t s , i s o

a - i l

o o n s s e h o n a a L t l e e o

l l m f f c l s l e s s , i r s . r o f

g i , ,

p p l o t e c u n l o

l s p b i

o s l e

c . h , . o e s S t S s m l e e m m k y o o

c l l d i , w l t h r e g l i o m

c . e i m t

e l a c

o i t s t c y a i i s p s

l a l p e e e r e e

o o

c l e l l m h

u l t c b i o n e e , , o s l e

a g a i e g n s y t t n n

a p c c w e l i i l d s a n x

r n

a c c a

, n t e l r a i o o a t t e

t e l u p

i c e g t u c o p a

c l r m o s d s p a k c c n h s l c c e s

b t r l e l l m e c r n t . r d a

c s h i e y y y , e d e e e s l o , v

a n s s

l y l l n

t t e r l e t a s l s

l l l o

i a d g a s . u e e e c e s s i y t l l v m a

a s s n , r e s c r c

t e

r e , n

p s s

o t u a a n

a e

a e l

o i d t s y e c

a a o n , a , n d o - t i c t l d n c d

v e n r , p r r

r e e l g m n n n d n y i

f b y o l i e e a

t d

d s t h l l m o e t r d d

r d t C i t l o l p g l t i c s

u i

r o a h

i a l c n e r e g n t a

v o s i h u

D o i b e e s c n d o n r g

a c c

d n r d a n . p o l i l l , r n s p p e

a i d u l a e

3 y

e n

d n

o s s o c d h i c T n e o i i u d n

l m t s 4 l d o c . t t e e o t p

l e l o e - h h e y t h c s k r

u ,

u t

l a c c h p

l a b d p t h l m o y e e u . e e l

l s h l

s e y o g a

r l l a

h e c t f l l r r l

a . e y , e i i t p

i l c e o c

a g a a r e o l a

l e C

s i m s c

s y h a e

n t e l l l c l s

t a i

u l

D . t l n o l i s a c c a s c o y ,

e l c

s r c s

o c e e , e 3 n t l c s

s a

e e . c y c c l l y l 4 d o . l l

n l l s e s s t s

e y l t +

r s e e d , . . l s

l .

l e s

s s

s ,

s . .

s a a B g i t T t F p i M N I C A M A L I d M I L A L M P d C B p s R M M R R E I A R M R C n n u a n m n n t i y - r r p c e u e i r u e d n d r i n d m e e r e e e e e o a

v f m r h v t o o o a y y o c o o n o o f m t u t r p u r u o g n l h g h t h o e u u l c c g c c n t i o e e e i a d d n y n w c i i d c l r n p m e b c n e o a k k p

e c e i e e e e e e l y r m t u t p l

l l l e e a c u u o o o i v

v i r l o o r l

i e b t o o g v n r t i t t a e

: c t s s p p p s p a o l h h o r i e i s o s e c

l l c c

g e m d s a b i i o o e n e a a c c s

g a m y i i i l x d d t t t t n t o

s p s t t l y y o o s o

e d e o o t o o u l e y y

a r n d t i h

t t

t f d T

u e

e v e e m d t t i c i e h e i

t o o n n n v

e n

c c n t t r r r r a n s t e s i n s

c e o i h - i a l e e d c s e y s s

n s i s i s r n ,

a e e t e c , d

t v l f f f f e r o a

o d

r m e

t t a

. i i o o o o s a

u k

l t c

e d r e l l a c a o m c s e i a o m a r a c n l

c t h n

l l a

o s s

l d r r r r e i

b c h g o s e h i o l

e c t

n a t

t f e l d t p h t e

r o s l i d t m n v o v o r i i t i i t h e

T T . l i o e I e u s o a e l c g e l v v g

d e e n i l h e a r e L g . l c e e a h

v l r a

i N N

u e a m s p a r m e t r h r

t t p e t p i d R c m i 1 y a a c c a d r e h n a m f o e n a e

l i l l t t n i t o e t l r t s F F i h s a e a b A t u o d e i t

o b s a e e o e d f t i i n r v e t n o i o a o i v o d e i r i . . . s u i v f a n l n r y l p h i t o o r o o , s u o

t g

l .

e t e v l n t

a y r

i i a e

p I I r s i m l i

s c

o r m e o n . t n I n i c f a n ( p n n o k l u a e i

r l

t a e v L e

e n x l r f e e e t o o o p N i s e n d y o . o . t , o v v n o l h e t

i o e t e s s

1 i a

r i

v t g i u i

i f n f s

o e o T M l , u o T o c f o n e i . a r o o d a v e n

T l

i p d

n e e B

k s b

i r c y n c h o r l - l R n t r e t a l a n f u t g a e h

g v v o

G t t a a r a e a c i o . a

a i o t i t

i o u r

a n t o i g t

a r n a n s e e e e n a i e n o a e c y n a m r y n h c c i o o n i m F n m o l t a l i n g n

t d d d

m t c

s f n

l o y s t n t s s e f c i n a a i d l i b

h c B

e i n i v c

n g r d

e e g p n t d

a n m

g a d e P

a i i e c c

o e e m a e a e r t . i n n d

1 o

n p p

o o g

n m i i

i n

a l d i t I c d g t n B m o c

l n r p l B

i d d

i u L

f . o y r t

i a n n g

a d r v p

c t c c e s

n h o a a a s o n w d o m P

i e M i n m c 1 -

d a e p r f a . v

t e e s e i v T v ) e r t

l d l f s

g d s a p g e

o R i i B i a

e a

R h d d n o p t l l u s e t f C o r o p s i

G f h

l l i n i t t h e u s n n e

o o n o f w r t e a i t n h

i M o N n l n l i o h d d f f o i e

v e s o a

v o

r l

o - f s e F n f d r o s l e i a m s P e n . a i e l o i i

n i

n . s

e n p l C t n e f f p • • • • • • • • • • • • • • • • • • r n a a r .

t i

i f

i E n t h f f

o t y o r n c T s u o i o M g

e b d o t m e e e r .

o i o

c n e a a s i t o

i t - e y . r n a g - s l a

o n e

n r a g r r o i t n n c

m t d n d n e

o i e o n

t e e i s e . n t

h n t a m t t

v o f e a c a

o i i

t h d i d a f h a n v . p n n n m o I s a d e p i a

a i f

k • • • • • • e d m a

c T d e

y i o

d r t o

t t n v t

a t

i r l c 1 t T a . k ( s e i i h g t

e e i c i s e a r u e G s a a i n o v a i e H p . o t . n i - r e

o c e e i m l c e l n t l • • • • • s d i a t t t c y l s n s p i o

s n

i r i S e i n n h l r T s

d s o o w r a

e o e l t c a . t i r e i r c

T c i -

. t a n a i

• • • • • • • t N e a o n n e u d l c o m . h t b I h j n • • • • • • • • • • • l a l u -

i s u L . u n

f d n y , , c n l h 1 P c f e e F r • • • • • • • • • • • • i t n a e

e u c n 1 d i

a e t e a a h e . o e i , s l t r s e

t r n n

h . l a c

i e t

o s a p B p p e l

H m a c s n t s i i l

f s e

t e

o n s p p f m . t l o y o i s t c i i g - r f a o S e

o o g n i s

m n

c e e o r t M o i p p o a

a o m e n

r o o n . n d V c e . c c n a n t t

n n u

n s

i t l o o p s a o e • • • • • • • • l e t c g 2 s i m a n e

n l d s f t . . : . o s s r l t l e e p o e r , n T

d i l s • • • • • • • • • • • • • • • • • • • • • h e , e

R i i a a f

n l r t p s s s p P

d -

l e i c s o a g n T s i n t t c a .

e h m r i i

t e R l

a a r o o . t . a - o s e i c s S t c a i u

• • • • • • • • • • •• • • • • • • • • • • • • • • • • • • • c n o u n n c n n i m V l

t l l e g u i l g m n u t e t f

n r d d o

. i ,

e i p n m d a a y

e u r l o n

m l B . a . s r a t n n a i l n

c n n t

o a u P l e . I H k n e e l p t d

o m

i g r • • • • • • • • • • t i l k n t r i o n a d c c i

l V p a o a

e f i l g p s l r r a • • • • • • • • • • e

t o

a l r n c t e 1 x o o e s

l • • • • • • g o n r r t i t • • • • • • . , s ) s s

c r i h i

t c . b h o

t • • i i i

a c o s • • • • • • • • • • • • • • • • • • • • • • • s - a h t e

l e n v

y t e . . l • • • • x c e l e r o

. • • • • • • • • i l

l r

s p • • • • • • • • o e

l d g • • • • • • • • • • • • • • a

• • • • e n l

i l l c s • • • • • • • • • • • • • • • • • • • •

a . • • • • • •

l • • • • • •

• • • • • • • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin Protein

Immunoassay kits CD121b IL-1R type II, IL1R2 B-cells, monocytes, macrophages, some T-cells and keratinocytes. Non-signaling receptor for IL1A, IL1B and IL1RN. Mediates negative • • • signaling of IL1. CD122 IL2RB Natural killer cells, resting T-cells, B-cell subsets and monocytes. IL2 and IL15 receptor beta chain signaling. • • • CD123 IL3RA Basophils, eosinophils, hematopoietic progenitors, macrophages, Receptor for IL3. Associates with the GM-CSF receptor. • • • • • • • dendritic cells, endothelial cells and a small subset of lymphocytes. CD124 IL4RA, IL4R Endothelial cells, epithelial cells, fibroblasts, mature B- and Receptor for IL4 and IL13. • • • T-cells. Low expression on leukocytes and progenitors. CD125 IL5RA, IL5R Eosinophils, basophils, activated B-cells and mast cells. Receptor for IL5. • • • CD126 IL6R, gp80 Activated B- and plasma cells, T-cells, monocytes, epithelial Receptor for IL6. • • • • • cells and fibroblasts. CD127 IL7R B-cell precursors, mature T-cells, thymocytes and monocytes. Receptor for IL7. • • • • CD128 See CD181 and CD182. - - CD129 IL9R Hematopoietic cells including T-cells, neutrophils, mast cells Receptor for IL9. Supports erythroid colony formation and synergizes with • • • and macrophages. IL4 in the production of IgE and IgG. CD130 IL6ST, gp130 T-cells, activated B-cells, plasma cells, monocytes and endothelial cells. Transmits activities of IL6, IL11, LIF and CNF. • • • • • CD131 IL3RB, CSF2RB Monocytes, granulocytes, early B-cells and hematopoietic Component of the high affinity IL3, GM-CSF and IL5 receptors. Crucial role in • • • • stem cells. growth / activation of eosinophils and in the inflammatory response. CD132 IL2RG Variety of leukocytes including B- and T-cells, natural killer cells, Forms complexes with other cell surface proteins including CD25, CD122, • • • monocytes, macrophages and neutrophils. CD124, CD127 and others. Complexes with other cell surface proteins to form receptors for the cytokines IL2, IL4, IL7, IL9, and IL15. Acting through the CD132 containing complexes, these cytokines regulate lymphocyte development and activation. CD133 Prominin-1, PROM1, AC133 Hematopoietic stem cell subsets, epithelial, endothelial and Binds cholesterol in cholesterol-containing plasma membrane microdomains. neural cell precursors. Involved in regulation of MAPK and Akt signaling pathways. May play a role in apical plasma membrane organization of epithelial cells. CD134 TNFRSF4, OX40L receptor Activated CD4+ and CD8+ T-cells, regulatory T-cells and B-cells. T-cell activation, proliferation and differentiation, apoptosis and cell adhesion. • • • • • • • CD135 FLT3, FLK2 Mul tipotential, myelocytic monocytes and primitive B-cell Receptor tyrosine kinase. Growth factor receptor. Binds FLT3 ligand to • • • • • progenitors. promote the growth and differentiation of primitive hematopoietic cells. CD136 RON, PTK8 Expression documented in many normal human tissues, including Mediates activity of macrophage-stimulating protein (MSP) which regulates • • • normal colon, mouth, prostate and testis, as well as cancerous cell adhesion, motility, growth, and survival. Implicated in the progression of colon, prostate, stomach, and uterus. several epithelial cancers. CD137 TNFRSF9, ILA Activated B- and T-cells, follicular dendritic cells, monocytes and Costimulation of T-cell activation. Regulates T-cell-dependent immune • • • • • • • epithelial cells. responses. CD138 Syndecan, SDC Plasma cells, pre-B-cells, epithelial cells, neural cells and breast Expected to play a role in cell adhesion. • • • • • • • • cancer cells. CD139 Germinal center B cells, follicular dendritic cells and follicular mantle. Unknown. CD140a PDGFR-alpha, PDGFRA Fibroblasts, mesenchymal cells, platelets, glial cells and Cell proliferation, differentiation and survival. • • • • • • chondrocytes. CD140b PDGFR-beta, PDGFRB Fibroblasts, mesenchymal cells, platelets, glial cells and chondrocytes. Cell proliferation, differentiation and survival. • • • • • • • CD141 Thrombomodulin, THBD Monocytes, neutrophils, platelets and endothelial cells. Activation of protein C and initiation of protein C anti-coagulant pathway. • • • • • CD142 Tissue factor, F3 Monocytes, epithelial cells, astrocytes, Schwann cells and Initiates blood clotting. • • • • • endothelial cells. CD143 Angiotensin Converting Enzyme 1, Endothelial cells, epithelial cells, dendritic cells, neuronal cells Converts angiotensin I to angiotensin II by release of terminal His-Leu, • • • • • • • ACE and activated macrophages. which results in an increase of the vasoconstrictor activity of angiotensin. CD144 VE cadherin, Cadherin-5 Endothelial cells and stem cell subsets. Cell adhesion. • • • CD146 MCAM, MUC18 Endothelial cells, follicular dendritic cells, activated T-cells and Cell adhesion. Involved in heterophilic cell to cell interactions. • • • • • • smooth muscle. CD147 Basigin, BSG Leukocytes, erythrocytes, platelets and endothelial cells. Cell adhesion and T-cell activation. • • • • • • • • • CD148 DEP1, PTPRJ Granulocytes, monocytes, dendritic cells, platelets, B- and T-cells. Tyrosine phosphatase cell signaling, cell growth and differentiation, mitotic • • • • • cycle and oncogenic transformation. CD150 SLAM, SLAMF1, IPO-3 T-cells (upregulated on activation), regulatory T-cells, B-cells, Costimulation of T-cells and B-cells. • • • • • • dendritic cells and endothelial cells. CD151 GP27, SFA-1, PETA-3 Platelets, megakaryocytes, endothelial cells and epithelial cells. Essential for proper assembly of the glomerular and tubular basement • • • • membranes in kidney. CD152 CTLA-4 Activated T-cells and activated B-cells. Negative regulation of T-cell activation. Contributes to the maintenance of • • • • • • • peripheral T-cell tolerance to self antigens. It exerts its major effects on T-cell immune responses via regulation of the cell cycle.

Discover more at abcam.com/CD C C C C C C C C C C C C n T C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D r m g 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 e e 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 5 6 t 8 8 8 8 8 8 8 7 6 6 5 3 6 8 4 8 8 8 8 8 9 9 1 8 0

d c f b f e b b a c a g k j i h c a z

1 2

2 1

K K K N N K B A A P C n A N A N C N N N N K N N N K B a I I I I S D D o l D I I Y K K D K K D K K K K K K K t R R R R R R m e l T 5 A A A A A A A A A A A G R G i 3 4 o 2 3 2 2 2 3 r - 5 e T T T T T T T T n 0 0 M M M - v 2 2 1 D D D D D D P

------a

i C A

l l 3 2 6 1 9 5 8 4 r 1 8 1 L P L L S L i i t 1 u g g i , , , , , , 7 0 , 4 5 5 3

v 1 1 A

s a a K K K K K K K , , , A B e

n n , R T C K

L I I I I I

K R R R R R d d A

R R e I D , R L 3 2 2 2 D

C c C C G w R D D D D - e L E 1 1 2 1 p B p L S S S 2 0

5 3 t 1

1 5 2 4 o 3 6 9

r A

, ,

S T N R

E A C P L

- 5 b c m N N N N M M e N N N U A C M S N A N N N N N N I N N P n y x l c p c e a a a a a a a b e a a a a a a a a a o t a o o o t p e t t l r o t t t t t t t i l t t t t t t t t t o r n n n i i e q l u u u u u u u u u u u u u u u u i r v v s u r p l d e o o o e y o u r r r r r r r r r r r r r r r r a a t l h i a a a a a a a a a a a a a a a a

a s t c c c n n w i T t t i t e e e c l l l l s l l l l l l l l l l l l r y y y o , a

- ,

r k k k k k k k k k k k k k k k k

i d d t t t c u l o

l e a e e e c y

i i i i i i i i i i i i i i i i e

l l l l l l l l l l l l l l l l e s n f h l T C m s s s l l l l l l l l l l l l l l l l

f e e e e e e e e e e e e e e e e l l p b e l , , o - y s D a

r r r r r r r r r r r r r r r r p c i l n m c

t . o e n e c c c c c c c c c c c c c c c c h h 4

t e d o o e e e e e e e e e e e e e e e e x a d l

e + l u r n d s r p l l l l l l l l l l l l l l l l c

o l

l l l l l l l l l l l l l l l l

t n i o , s s s s s s s s s s s s s s s s T

r a r u r c

n e o e o . .

. .

. . . . .

. d a - c m y a a a a a a c s p u

p e c t t n n n n n e i t s e

h t h t v s u a r i d d d d d l e v s a i i o , l r t l n s

d s a a

y t g T T T p C γ a . d h .

. t l δ e - - - h a

n D

y c c c k l s i n d i d m T l e e e i v 8 s , l d

- l l l e e . + m f t l l l u c

e h r

e r s s s

e s

t a y n u u u n c a , l m - l

c a e b b b s d l p c

r . l t l s s s o e e o o i l u v s

e e e t l r p c l r h e

i s t t t y a p a h s s s e r . t l . h n a . . . e

i k

e d g a s i r

e l l

l C

a a e c s l n r D e

d c l n 8 l l s

e o e + C . l o u

N s T d t r

S a o c l e n p

e n u d h l l e k s

i l p u o s

w e r c

a o r y i t i n n p t h e d s h

s .

B ,

r b - a c o l e

l l e s

m s t n o o o o k k n k n H a k v S R C R R F R R R p C P B S R P M i M c M M p R R R R R N P R I r R n h n e o i i i i i e a a c a f f f f r r u e o i y l l l l l l r L e D e e e e e l e e e e e e e e e v u h o e H e e o n a o a c l l l l e u t m n n n n n e e e e t t t n n c s o A c c c c c c c c c c c c g c c i u u s u o i l d t d t d y l y 4 a v s b C e t a a a a i r r r r a e r c s t e e e e e e e e e e e e e e l f a

e e s r r r s g - i i

h s i v + e v e p i c a a t t t t t t i l c c c c a a o a C

a t p p p p p p p p p p p p p p t b

l r

u u u u

y i

n e e e c s t

o r u i o a r a c t t e e e e t

l l l l T e v t t t t t t t t t t t t t t t a l e e e o t

r r r r w i d l

s s t o o o o o o o o o o o o o o e p y l o n

t h k k k a l l l l a a a a n e - a e d l l l l r t r i t s s

s s s s o t r r r r r r r r r r r r r r c

r 7 i i i i f i s s o C r o s t i l l l l a

c l l c l o s o i

o e n

r o l l l r

n e t n o o o o o o

o n B o o o o o o s ) n s k k k k e e e l l a t t t t h

o e t n o D e e t n .

u h h h h r u b v l o h

i i i i n n n n n n n a n n n n n n a e

a r r r - l n m e l l l l

g I o . f

I t s

l l l l o g u u u u r 3 c y t

x o

i a n

t a i e e e e n c c c s h l n m a u c n f n n n n n n n u n n n n n n . u e

e l h s s s s i 0 e

w s h s e e e r r r r v t c m n y e

l t l g a a a a a a a r a a a a a a r i

l a

m h n

o b e e i

l a a l l l p p p p c c c c a k c i i a

a b

l l l t t t t t t t t t t t t t T n n e s t e s - s e t p c l o

u u u u u u u u u u u u u m e e e e l l i l r r r r t i e n . e

i m

l i - t o g e u m e e e e

t e x l c r

r k k d r r r r r r r r r r r r r l l l l e c t t e s c d M d l l l l e c o e a a a a a a a a a a a a a n

r h h t v v v v

s s s s i l i e y e r b A o

l r l u l r a b

t i c l l

n l l l l l l l l l l l l l l t

e e e e a u u . . . . a a i

i i

H e

e d l l h o r f n v l f l r c D e c l y k k k k k k k k k k k k k e t

n n n n c o s s e s t c a r r i

s e i k f d e C i l s i i i i i i i i i i i i i a p t

t s

n a . t t t t P e l l l l l l l l l l l l l a r n n y c e i c l l l l l l l i l l l l l l n p p

i i i i u l i a t l t e e e e e e e o e e e e e e . n n n n

l s a g s l , d l e o e e -

v c l a r r e c

t e m u r r r r r r r n r r r r r r . r g g g g s c

c e e

i d r

l e l l

i t e r t o s l a l f x b l

c c c c c c c c c c c c c t

u e u

s v v

a o

r s f w e i c c c c s p a n c t s c v e e e e e e e e e e e e o e o a r l

e e m r w r r e e e e l d

t e e d a

s y i i - a l l l l l l l

l l l l l r l m s p l h n n t t a l l l l l l l l l l l l l

m c l l l l i

y l h

c t s s s s s s h s s s s s s s o i l l l l l a c e v l t r t

u I o t t

n e s h

c k i

o i i

l l l l e r e a t

f e o n n o y y y y f f f f f f f f f f f f

H s B t d

l e a i h i e o o o o o o o o o o o o M l o s l a c c l l s s s s s d i f

g g f

l l

- f d n L i

r r r - r r r r r r r r r e a i x e i I a n n e i i i i T c n o r i

t a

g s s s s u

d A r c c y i e r n H H H H H H H H H H H H l t d e g r c c a n N u l e y . . . .

E i m e e

c o d c - y

i l , i p f L L L L L L L L L L L L t

r v t l E n s

F o l l

t u i a o e s n

f y t l l m n p u

i A A A A A A A A A A A A e c a

o o v o a r .

- r

n g l

l l h l r t m e e

n y y α a

o c t

------a m - - - - -

n o a n b o d n B o a C C C C C C C C C C A i s s x l t e l f s b

d l t d

o o i d

x e - c w o t i i n p

t i k t

s s i v o m a i u e

o r t l p u i a a a a a a a a a t t o

f i e g r i c h i . .

4 i e l c n

l l g 1 u n e v r c l t l l l l l l l l l T l n a g i a c

e l l l l l l l l l

h o e

t v e e e e e e e e e c a g , k t c

s a i y -

t u e t

i r a t n e t l o o i e o c l l l l l l l l l r o l 3 s t e . e o

e l o e e e e e e e e e a l n i

i l n c v

e l e f l o i x n e c , I r s t a v i n l n s s s s s s s s s

s e

n e o n o d

s s M

l y n

7 l . e l c l . .

......

s i g e i r y

d l

l s s x

(

o n t l . v I , a t t m d i b I I I I D D D D e s H

. H s

h i u c i n n n n t f C

e I c v a s • • • • • • • • • • t

e y

t s i I m a h e o o o o e c i L i s t h h h h a C h n

i m u n n g

D h r . t T e l i

e e e e i n . A y i i i i p a e h

b • • • • • p c r d

t r

b b b b u - a e 4 s t h

c s s s s a

a o c

h i c i - l h e

o i i i i s b t p i n

l n t t t t C 8

0 e t t c t s p e a

i s s s s n n n n e f u

f i i i m r a d t f

o p

a t

t l w s

e a o o o o a

s n s r t l o t t t t s

i s n s n r h t a s m n h h h h n s o c

l t t t t n a e 1 e

s l u . t

, , e

l t o e

e e e e o t l h i i i i

t d I , i v h p u n n n n a o p r r s k u

t n

n e

e H e a

e H e y r a a a a b h h h h t r p

i a r i c u l e - i

c n c p s a l

n c c c c l i i i i L f r a L c c e e H b b b b e r u -

e e t p t e l t t t t

l A e t t c A B i

r i i i i i

i i i i

a t v n i h n p s k E o v v v v t t t t s

a o h v t

c - - - s i i t c s i e g i i i i t t t t t . n t a c v E f R C e c l t t t t h o h h h h

e y s l

t i y y y y

I e s t i I e t o

i y

c t n r

e e e e l i i 2 w L o i

g • • l c v y i o o r

l n o o o o s m • • • • • • b e h

f l

4 n e a e

3 f a a a a o n r c

f f f f o l l g

i e a . s o

e l r o l

e c c c c r

n n n n

• • f f m r a A o . l

c n f • • • • • •

c i t t t t y o l

a a a a f t l i i i i n

f e • • • o d s l y s v v v v r s i w

o s • • •• • • • • t t t t n m d p

u u u u i i i i r

i o t t t t t l e s

a t

i y y y y h t

r r r r t h o .

. o • • •

h a a a a l

• • • • • • • • • • • • • e

• • • • • • • • • • • • • • • • • • • r e l l l l • • • • • • • • • • • • .

• • • • • • • • • • • • • • • • • • • • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin Protein

Immunoassay kits P ro ® ra d n u Accurately quantitate CD markers with Alexa Fluor conjugated g c e t Abcam ELISA kits secondaries s

• Excellent sensitivity • Tested extensively in our laboratories • Wide choice of immunoassay methods - Guarantee bright staining and low - Sandwich background - Competitive • Large range of pre-adsorbed antibodies - Indirect - Ensure low species cross-reactivity - Direct • Dilution range of 1/200 – 1/1000 • Broad species selection (10-2 μg/ml) in IF/ICC or 1/2000 in - Human flow cytometry - Mouse - Perform at least 250 stainings - Monkey • Antibodies conjugated to Alexa Fluor ® - Pig 488/550/594/647 - Rat - For your multi-color imaging needs www.abcam.com/ELISA www.abcam.com/Alexa Wheat germ DRAQ5™ a better alternative expressed proteins to Hoechst/DAPI

• No photobleaching effect – photo Discover the unique benefits of our wheat and chemically stable germ expressed proteins over conventional • No RNase treatment required expression systems • Half scan times of Hoechst /DAPI • No compensation needed as no • Animal- and serum-free overlap with GFP/FITC and PE • Of eukaryotic origin and therefore complexity in combinations in flow cytometry shape maintained • Quantification of DNA content/DNA • High solubility due to native conformation and specific eukaryotic source • Stoichiometric – applications in cell • Greater stability cycle analysis www.abcam.com/proteins www.abcam.com/draq5

Discover more at abcam.com/CD C C C C C C C C C C C n T C C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D r m g 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 e e 6 6 6 7 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7 8 7 7 7 t 8 5 7 0 9 7 2 6 3 3 4 1 9 9 8 7 5 3 6 2 2 0 2 4 5

a b b b a a b g s

H A D S S n A D P A M M C N F N T B λ V T S S R S L S a e 5 a n h D I i l S L l p H I I i M D D D C B P 1 U a t o a G R R m w e s o

C r G 2 3 1 P 1 l o l R R 1 A e M C o y

P P r L m i l , e A 0

0 d n 6 s S B i l M L a 1 2

g E - g

B G R g 5

a 3 s T 2 M - d - l 1

Y g a C y - 1 1 p e 1 t , L 4 n h L

r i

n c

v 3 n , S o 5 1 ,

e 1

o d

e S 7 - u , , M I s ,

p , R

O M p

i L C R r n G r

o i 1 B H 1 e , D P C

t B C 6 S d e -

A 9 B t - 2 0 e i A y i 2 n g 5 P

n p M

4 , l

e r 2 l e H i e

g c

2 i N a - c 1

n h A

- a 2

n a t i

g e n

M H a p L O e C I A M M M M I T S I e e m P P N E A M M M C S S E s s s s t y - n l x x p c t r r r c t t r i e e D o o o a e s o o a o a a o o a

g e e e e e y y m p p d i a t t t l u f f f t t m n n c n c r n n t i i 4 t h l m t m m e - - o o o e h r r v v u

u n r h h t p B B r r e e n o o o o o + o e o r a a r r r l e

o o r l d r r e

h o c a m m m s s e c c c c c - - e w c s c c

o o c t t l p p

c c a t i e e e o s s p r y y y y y u B a e t e

e s y c b

h h e e

p n i e l d d t t t t t c h 1 1 2 s r e

. t l l l y o l

- l e e e e e l l l s a a a

l l e e o a d y d

t i l l : : : I t x n l T T s s s s s s s s s s r

. s e g g n s r n s s t

p m S o p

e i C . . u u u

, i - - o , s p . t e e d s n a a

c r m

r s

m l

g g b b b a f o o , o

. D e h s s

n n n e o

, s

e h r r s s s n s

n s , . s a u s e a d d d a a l 8 u e r

p e e e e l t t d t

n o s m l s b r l

e n n e + a b n

l i t t a a t T m d c i

e , t c s s s s o m u u o B a t s d

r l - e e T e y d u e 4

n t a c e l l b n o e a a

B - o o

n l t

e t t e : l c e a e

c c t c l d t r c s l

s n n c c a i - d s i h n H r o e n r l a e n a s c e , .

d d l y y y , s o r

l e d

p l

l l e d a n m e i l s l t t t

l t m a t t l p s l r

h e e e e h c h s i i l

r c i u m s c i c l a n l , h o g t e s r s s

p p i e u o e

b u i

a o l d m c s a n e a c , , , c l

i i

n n r s l b

t t n c b c

s e

g m m d e o

t h h g c m a o o e t s b o e y d r . u l e e l c e e e . l a n c l n t e o o p t o l

s s a s m y l s e n

l l a l y s i d o t i i n w o l s

. n . a a t c . s s s d a c t

c o t

o i e a , r

l , l d e

r t n

y s o T a r i c i l c c s n t e l v t t u s d t , u

n p - n i y i e e

d a c a c n

n a

i m d t h a d a m b l l

t e d l l g n p e c e s s

a n

n o ,

p o s r e , d

m w e r . . p d g d i d

t n o

l r t p p h

a i a i

l n t e a c g N s c o i B l y h t n t a s l e

e t e , e h l s m c e l K u -

d . c i u a l b c n l e

n s e e l r l

I e

i i s r e s e r e d s i l c a i c l t t i o n a n l

s a

o o l s e

o e

l a l l n t

g e i t s u . l . r f h c n l u n a n

l o s

c n s e y c

c s d a

d . r a i e a . g m l e e t

r n

l h m n

l n s i t

l l l t d e a l l s e o d i .

d s s c e 2

s . n l c

. s

t a

: t k a d c y A

a t

e e i s n e l t

d s

s l e t p m s c d l h n t . l s a o s e l

e e

b , . s

l c

r l e y o s

o e e . n n

i s l d

l . e s .

d H N i a b b o i a m t m d m m m v F m M I T E M C B A a T C A r b a M o E B H M P T I I M A I M o B C i n n n r m m m m e a y y i i s o i f n n l n f - f a u n i d d a - r c i y F l l i D f f c n h o v

i i y a a a e a a a e a n m c n e e s c e f f g g r r s s r b c p m d n m d d m n n t a d h h r r a e e d i o k o o o e e c c y d y y j d y t d s d 4 e a a b c o l c e e r r o o o

o s o c y l o e e s r r B d i b r r

a a s s l r n r

c u i u u i u s s 7 u l r r u n i c a l r e e p p p a r a b f n a o o t r

l - e e t d i

c s s a a i i

e o e l t t i

m e

v n n n s t r b

m l i r n n g a o s C n n d l l l t t e i i i E e s C a p p a a a g g e y i i b o a a a n n s o o m e e r p b e

o o u o o o l e e - i

s n

t t e

m o c h h t a n r t u u n l i n o - D e w y y y c c D c n n a i i s s i t m e n i i n n r n e g g g

t a a ,

n y m n s c v o t o a a r t l l o s g

a e e r e

k k d

n i a a l e 1

d a

d i l l l r 2 m a a a P t t t v

t v

a e l g r t o o o a m m u f d g g n i i

a o n

i i o l t r i h p

h u t t s n n o

3 i t

o o l a t 2 a

n a a o o e n l o

n - e e b b b

t r i r w

e e o a r r o i r w

k e r o s o

n d y

a a r g o o 3 g r n n f t , t l

r n n l o o

c d n o C g s s u u u a l v s s a

i i i o t p n e

r i f n

o . i

n o s s m r l l + a f r S , , h d d l e l t i o f

c l a e e

o e d . . l l l n e

c e e d o n a w h D m i e c f c t c c i i i p

e e g e n m s d

f e

n n n p e o f h i a d c c s d i e d t p c e

u n t e e e g e

e e

u f r

u e

a . 4 i i h c i p

t - - - t e r f f e u u i n t t h n n b

e e p o

A o o r p a u p n n l n o l l l e e r l l l e A h h i r n L y l 7 e l e l l i i i a o n

n l l o

d e

y l v r n k k k v r r

t m t e e d d e r t r - e n l l d a a t t h p a d i - . c o l s r e t o p t o a s e t

c e o

e h

s e e e i s

s a i e

o o

. m x t v a c t u m t t e r t i - a i o d

r m i h k r l o

v h • • • • f i i n o

t s n e t

e o n 3

a i n . o t l a a m c c a f

f f o n a r c c c m m n n a a i u

e r o r e p e , u u e t

n t

e n i l l v - C n y y t n t l e a

n e e e s i n i e x d s b i c d , t

n c

5 l i g a n n o r f r

n n d

t o a a m t t

s e o a r d

. d e i c h c

h l l l e g c o o

i a D o o s t

t c

o b l l l

e t v e l m n c c B i

l s i p t

d n n i a e

e O e i a i r t o m v n o

g f i o g s o s s i s s s u o t a o l y n i t t 1 u g d a t

e t r e -

. d h n n c l r i i t e m a h t t t y n n i i f u u u e p l c • • n n o o o

v t l b i e

6 r s s n i h a i t e

- u v u n

i i s . d n a c d t i e i e

o r r r a n s n c o e i c e . n n m

o t s 8 u a y s i b n e n l

f o f f l g i I e o o

g e n t

x o s e n r e , g a a a a i r n

s s 6 i i n m i i

r l i i y e t d

s o b o r s

n e o l f f i n

g a p r i

a p u w

d l

c c c l n c d

e . o o s .

t l

s s o

s a a

d o n

h h

m o c r o m a t i r p e e e f l i d g t m . a u I e s R f p u p i n . i a n i i e f o o s s p i

h m , t e e

s t a o

n a l

p , h x c T s i r t r P

e a t t g n s a

r r r r e s m v r r

. y e m m y g l n t h o o h a a d l e e e o e r m

e i e p - h

s d t

e t t a r c d q M m l t e o d r

s u e l c r t a r

o c c c n s o e e n m s l i a o o i o e o

o c c - u m i t i i r u f o a g u i t e n s r

x c t e e e i

w a e a s

m o d s a y s e n g g f

e e e o w

e e a t g i n t u p o i s

, l a - C r r p p p e e

c o e r t g i d i t i p d i l l

l l x

n d

p o t a o f e e r e m o o

a o t s l l l

d c e t c d i o t t t d t e b

i n u h o D a s t

d e

e o o o o a n n t p t d s s d

b b e t t r B e a x b e

l o t a

i a e l

w m n a

d a u u t p r r r i p s /

o . a 6 h i

i a i i l p l d n y n o h n - n

h s n n

l f

t f n i l s t c

s d r r e n t b t o n i f t i h t .

c i n i a r

e i c

a m n n

l n y d h o

y o h e

f f

g t n e H

e s t r e i e n I g / / a a a r . m r i d t e m p

n m s p v g

a n r g ,

s r a s e e

u l e f s o i

h h l a d

c c c i y n

l t

r u i g o e l f i e t

f u p v e o t m C y n h u g s s r s v s e l

i p i t a t f e e a a a t

e o a c c s e r m l n h o e

r h m l i o n f ,

s i e g l e

v m a d g a D r s

p p p o n i

t a r i

i m

u d e a l r r r t

o g n . . I o o e c e

h r m v d c r e

e t h d t t i n e e l e n t t i 4 a

• r r e a b i a

o o c n

e n

o d p e

i o m

e t m n m o o e e e F v c c d a

c t a 7 c l a n l r s e g g i d n l

i d

n n n o e e e l l n

a c u n t i i c i c a e L l . a s i l s t a s n i e a n l l h u e o

e a i

, g d

o o i

p p t a n l a d n b s l i . S P s t v t s b n a e t i t x t

e l n c s i

a i v r r b b

e o t t r t n e e r o - g o C o i o e e t i a o a t i x o o h u t i S o i l

c e a n s t r s i i i x i s

t . n c

n n r d o s n n i a o p t m t r a r r e p X r i i n o • • • • • • • • • x c i

t , d

c t i n y m

s e e e . i . e

m o n

l c n r a c

p f f p n

p C i i c c

t o e n

y o o c o

u g n n c

e d s n ,

e o c a o l a r

d o o o

a o o e m e s e r r B L f e r u

e g t n l

l s n r

l c

l e e i m m l l s o l f s t u

l - g B t 1 f f l e u t l s

i e i - m

l u a l d a a e e i i g c s s i t m v s i f m s c b b k a t s 2 u l o o p p -

u b d e

a i s t l

. n e

. u e c o e v l r r t a e i e t a / k

u l l

r • • • • e n e

h u l i r i i C a l c e e c e m e l c l v n a a l l o d s x o

n l ,

l l l t g s e l s e m e n a x x a a

a l y e l t a t l n

X t p h c d l c y

b . . c

e . s l e e l c r r r t d m

s t

l d i y

l m e e

C e

r i s e n

i a s L . n y r s s s l c c h t e t

s l i e t s . a

i . l f e r e i l c s

R s e r o o g l t e s

i g i g b b • • • • •

g l i o s o a i s a u t o x n l l r

o s 4 u e y y • • • • • • l l r u i

a . n l a a d e , s v t a i t

t o . l h c a t a

g g • • • • • • • • • • c

s e s a t i

a c n i o o e a t x e

• •

e e o r t i e t b n e

o r n

i n o l n n n o s l d • • • • • • • • • • • • • • • • l u d s

l d

n s

• • • • • • • • t

• • • • • • • • • • • • •

• •

• • • • • • • • • • • • •• • • • • • • • • • • • • • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin Protein

Immunoassay kits CD181 CXCR1, IL8R alpha Neutrophils, basophils, natural killer cells, T-cell subsets, Neutrophil chemotaxis and activation. • • monocytes and endothelial cells. CD182 CXCR2 Neutrophils, basophils, natural killer cells, T-cell subsets, Neutrophil chemotaxis and activation. • • • monocytes and endothelial cells. CD183 CXCR3 T-cell subsets, B-cells, natural killer cells, monocytes, T-cell chemotaxis, integrin activation, cytoskeletal changes and chemotactic • • • • macrophages and proliferating endothelial cells. migration in inflammation-associated effector T-cells. CD184 CXCR4 T-cell subsets, B-cells, dendritic cells, monocytes and endothelial cells. Cell migration, hematopoietic progenitor cell homing and HIV-1 cell entry. • • CD185 CXCR5 Mature B-cells and Burkitt lymphoma cells. Binds B-cell chemoattractant BL. Involved in B-cell migration into B-cell • • • follicles of spleen and Peyer’s patches. CD186 CXCR6, Bonzo Th1 cells, B-cell subsets and natural killer cell subsets. Receptor for CXCL16. Involved in T-cell recruitment and is an HIV-1 co-receptor. • • • CD191 CCR1 Monocytes, macrophages, lymphocytes, dendritic cells and Binds to MIP-1-alpha, MIP-1-delta, RANTES and MCP-3. Involved in • • hematopoietic stem cells. leukocyte chemotaxis and affecting stem cell proliferation. CD192 CCR2 Monocytes, B-cells, activated T-cells and dendritic cells. Leukocyte chemotaxis and is an HIV co-receptor. • • • CD193 CCR3 Eosinophils, trace amounts in neutrophils and monocytes. Leukocyte chemotaxis and is an HIV co-receptor. • • • • CD194 CCR4 CD4+ T-cells, basophils, platelets, macrophages and natural Chemoattractant homing receptor on circulating memory lymphocytes. • • killer cells. Co-receptor for some primary HIV-2 isolates. CD195 CCR5 Monocytes and T-cell subsets. Leukocyte chemotaxis and is an HIV co-receptor. • • • • • • CD196 CCR6, CKR-L3, GPRCY4 Lymphocytes and dendritic cells. Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and affects • • dendritic cell chemotaxis. CD197 CCR7, BLR2 Activated T- and B-cells. Strongly upregulated in B-cells Receptor for MIP-3-beta. Mediator of Epstein-Barr virus effects on B-cells • • • • • infected with Epstein-Barr. and lymphocyte migration into lymph nodes. CDw198 CCR8, CKR-L1, GPRCY6 Monocytes, T-cell subsets, dendritic cells and HUVEC cells. Regulates monocyte chemotaxis and thymic cell line apoptosis, is a • • co-receptor for HIV-1 infection. CDw199 CCR9 Thymocytes, lymphocytes and melanoma cells. Regulates chemotaxis, is a co-receptor for HIV-1 infection. • • • • CD200 OX-2 Thymocytes, B-cells, activated T-cells, endothelial cells and Down-regulatory signal for myeloid cell function, costimulates T-cell • • • • • • • keratinocyte subsets virus. proliferation. CD201 EPCR Endothelial cell subsets. Enhances protein C activation, involved in the protein C pathway controlling • • • blood coagulation. CD202b TIE2, TEK Endothelial cells and angioblasts. Regulates angiogenesis, endothelial cell survival, proliferation, migration • • • • • • and adhesion. CD203a ENPP1, M6S1, NPPS, PC1, PDNP1 Plasma cells and some non-lymphoid tissues. ATP hydrolysis at the plasma membrane. May also modulate insulin sensitivity. • • CD203c ENPP3 Basophils, mast cells and their progenitors. Enzyme catalyzing hydrolysis of deoxynucleotides, nucleotide sugars and NAD. • • • • CD204 MSR1 Monocyte-derived macrophages. Endocytosis of macromolecules including low density lipoproteins, host defense. • • • CD205 DEC205 Dendritic cells, B-cells and thymic epithelial cells. Endocytic receptor enhancing antigen presentation, causes reduced • • • • • • proliferation of B-cells. CD206 MMR Macrophages, monocytes and Langerhans cells. Endocytosis (phagocytosis and pinocytosis) of mannose-containing solutes. • • • • CD207 Langerin Langerhans cells. Facilitates uptake of antigens and is involved in the routing and / or • • • processing of antigen for presentation to T-cells. CD208 LAMP3 Dendritic cells. Dendritic cell maturation marker, involved in cell proliferation. • CD209 DC-SIGN Dendritic cells, placental macrophages, endothelial cells of Mediates antigen endocytosis and degradation binds pathogenic antigens • • • • • • placental vascular channels, peripheral blood mononuclear cells such as HIV1 gp120. and THP-1 monocytes. CD210 IL10RA, IL10R1, CDw210a Monocytes, B- and T-cells and large granular lymphocytes. Receptor for IL10, inhibits macrophage activation by interferon-gamma. • • • CDw210b IL10RB, IL10R2 Monocytes, macrophages, dendritic cells, B- and T-cells. Forms part of the receptor complexes for IL10, IL22, IL26, IL28, and IL29. • • • • CD212 IL12RB1 Activated T-cells and natural killer cells. Forms part of the receptor complexes for IL12 and IL23. • • • CD213a1 IL13RA1 B- and T-cells, monocytes and endothelial cells. Forms part of the receptor complexes for IL13 and IL4. • • • • CD213a2 IL13RA2 B-cells and monocytes. Forms part of the receptor complex for IL13. • • • • • CD215 IL15RA Widely expressed. Receptor for IL15. • • • CD217 IL17R B- and T-cells, monocytes, macrophages and granulocytes. Receptor for IL17A and IL17F. • • • • CD218a IL18R1 B- and T-cell subsets, natural killer cells, monocytes, granulocytes Forms part of the receptor complex for IL18. • • • • • and endothelial cells. CD218b IL18Rbeta, IL18RAP Natural killer cells, T-cell subsets, monocytes and endothelial cells. Forms part of the receptor complex for IL18. • • • • CD220 Insulin receptor Widely expressed in tissue targets of insulin metabolic effects. Tyrosine-protein kinase activity. Binding of insulin stimulates its association • • • • with downstream mediators including insulin receptor substrates and phosphatidylinositol 3’-kinase (PI3K), which leads to glucose uptake. CD221 Insulin-like growth factor 1 receptor Wide tissue expression. Mediates insulin-stimulated DNA synthesis and insulin-like growth • • • • • factor 1-stimulated cell proliferation and differentiation.

Discover more at abcam.com/CD C C C C C C C C C C C C C C C C C C C n T C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D D D D D D D r m g 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 e e 5 2 3 2 3 3 3 3 2 2 2 3 3 2 2 2 3 5 4 4 4 4 4 3 3 4 4 4 4 4 4 t 2 6 0 7 5 6 4 1 9 8 3 5 3 2 4 5 2 3 9 8 5 4 1 9 8 3 0 7 2 6 0

b a D C

D P M G G D T L M L n A G B O M G I P A E E p 2 R L K M B T I A B F C s y A a 2 B u C r l a l p A n e l L l N u H l l l G X U e 6 D I t e o o P y p y y A 9 m 2 t T e G n o d 4 l K f R P h l P x l o o A A 4 c c c f a C R T a

M 0 r

M d - o , y , b i e e o o o d d

n - 0

n 2 n M G

n 1 /

N 1 - 3 Z

s A l 2 r -

p p p

a a L 1 3 1 o - L - o

a g g , 4 i , ,

7 - C m A a 4

h h h n t

, ,

o c , t

I n r r i P

, r

v G l 0 A o o o t L o o h I T 1 d

i a , i

i L n T U n e

g b H r r r e u u a , E

P F n i i i

A g

e n n n l u p M p V i 5 o s 1 I n T A

r p n L I - - - -

o f 0 E o - A B C A R R ,

1 e I e

L R

L u d r S E

3 1 r p / h h A e

p D r

, P M t g ( ( i c

i n

D C D d 1 g r e

- , A o R a

l ) E p

S y M

u s p t

) c P o

p e

F o o p A r

, l I a A p o y

2 2 D n r l p

y a o

t A e p i t

g e p R e e i t p n i C n d t

, i e d

B e

C A M

B T c o T T m e E A e T E E C L T T E E D T M N A E T S T E E E T E E E E e y x - - - w - - - s - x - u r r c n r r r c r t p r r r r m r e e a e c c

c c a e o y y y y y y y y y y p m l t p m a a a t t d i l o n l t e e e e e e l i i t

s t t t t t l t t t m t t b r a u a r v v n u n n h s h h h h h h h h h h o

e p o d l l l e l l o t r i d r a l l l a l l n u d l d d e r r s r r r r r r r r s s s

t s y h a b a s r r s c c h o o o o o o o o o o

t t o b o

l , ,

i o a e e o s l l r i B B B

a u a e e t e e c c c c c c c c a c c a c

s e n m f k n i i n d d c o n b n l n y y y y y o y y y y y

l l c - - - s l y e l d a i i

l i y t c c s c

a l t t t t t t t r t t t o d s d c d c n s t t

i T n l t

e e e e e e e e e e t m g s t e e c e e

e i s l e

u e n

e o . a n - a

n s s s s s s s s . s s e n e s r e l l l . c c

r l o l l l s t s

t l . . , . . , , . . . s s s s e

n a , e h a n e l e c s u

c l d e . , , e S . u

p m r d t e l i e

s l l r

y c l u y l a

m m n n a s k r s r e o l l , d t

t i l r o o o n

, a i e d d l e

h s o r t a a r l

o o a h n n l d d t k s u , l s r o o x e n o c l

n n n o

e o s i u t

c b , g p t t r l k o e e l t

l o o h h d c

l c e c e m i a

i i o c c m n v y l r a c c c e e e

y e l l r s e n e y f l e t d a e a y y

l s l s l t

d e o

i i a t s e a C l r a a c c o t t t

l c s l e s

l e e s p a i n e s t s b c e e s l l r t i N s u ,

o , h s s h n o , d d

e c c t y

l l ,

G r a l l a m , , n S e i

B d p s

s e e

a l

l g T l n B a s m e n . l l s

l l - l y i o a r s l l - a

d a l n t c . s s - a a t k a c c

a n t n e

n c

l e t

d r i o e n

g n f u a a c l o o d c r d e o l l o p l u e l e d n m r n n c l e s m

l l s o t h m l P l l s r

a y d d h

i o l s m .

c o l p a o , a c t t s N

e , i e u e e r h n r e v l e . o l y

i e l S s l i n

p p c a d f a e p a a l n t i l n

- b e e i i s . g

i a r l o t t D t n s

h h d

. r s l h e o c n d l c p o a s

e o e e s m i d y e a l s b t . l l i

l l u t t i i c

n n

l i a l a a e h a p s a u a r i d d n n l l a , s

l e l b s

c r

c c d g . o c l i l t f i

e q

t i s t i e e k

e : e s b a i s

l u c . l l i l G t l l r l l l l s t l e e

s s l

o s e t e c c . o l t . . o

b u m e

s r a e u

l c . l c a n l s l

y s l o c e l s s d t y . p e e l t

l s a s s h e l s l . s t . n

x o e

a . p r d m

n i r n

e d

- s

e i s d

s p a i i i I I I i m f H c r t l a a M F M c a I C I P C G A R L T s I R A S T N I P P Z C P L L T P s n n n y n n n h n n n a n o i y e m a y n r n g i y r u i P i m o n i a l l a g l m e e a e e a o t

g l v v v e l v v h n i i i a g o l g a a a a t y c e t t t g n m r m e t d g d n t i r r o n

h h i p l t c c t n o o o o o a i

i c o j o o l n y y n n m a c a l e i b n p a u t t r b r o o

i p

p c e e s a

f e e a v l l l l l

n a i i a a t e s s s a o e n a s d t k n n v v v v v o o o n i n e h l r e i r r r h t a n e o k y t p p e l

t a

i d c p t

i l p d o p g

l e e e e e

s i t

i f n f

r e e d o i o a n i a i s e e

n e z e r t f n d a s n t i m

t t r m o t a l

t h o h t a r o

h a d d d i d d u a a o o

e i r r k t i n

f r

r e

s e

l x i c i o a a k e s n r r o t y y f u e c o a a n r a y e l r o t

m n n i r r i

e i s o

o l o i o s c i i n i t n i i i i i i n n m

o l k A t t y

d r . e r n m l t t o r n n n n n l n g p s s o r a

p h h p f f

l l

h p a h a l l

i r e a i a s

s o o i n e . o o s R e e t l

t n i n

s n o n g i n a l h r r

h o v a h r a e

l r l p n a i c l c n . d

n l l

r r r r

t o o n y d

, o a s i p o i s - a e l i g a a t e p r i i i A

n e r e n p a g e g

h y o e d v u r n n C i o m c P S a c c t u r u e e c s n o t l - d i u n n r e

c l

g t s o o c o o a n u h e 3 o o c

e m 5 y y l n t a o l i y c e r

c d l E n u t t s d e d t o p r u s r a e l r m e r m s y t

r e c 9 f s t u h t t

l r s v t n t a t r c

i o

a e e e l . o r . e e l e

u a M a e p h i y e o p s s e c r r d i p e a s R m e p e o e d e s d n a f e e e

c s g n n ,

t k s s

m r o i n h i i p t

t a r

t o m m

c n t n d i

p i a o A l

o s c e p P i i r c r i u s

f a a n o o o . e e l o o c s a

o m t o c e f r u i i i

e s

n f n t u o t o e r

o c c i 7 e l t s l t e e r - a c

i f y i e r i n n n e r r t a p

o e l n g n r , c t p v

g t

p d

o r p a p

n d o y s A r y

t r t m m n i a i r b c c

d i T t t

r n l a n e

e n e u p t . a n l m I

o i o l

e e t e , r l s f e t s c i y d o n o u , a e I o i

o d o e

N t s n

a n m r c b b

n y

a f r i n s m s a e v a u c i a

s o i m g b t m m t , t o f e e r o r

i n a z l t s c o r r

s e w

c F e

o a v l i

d p

c m i p o l i l t t a a t r l n a

d n b f a y i o o i

t o e p s

d h h p

p a i n l

p o d f R t h t e e e l p r a n m o s n n g n i h m , e a i l t a o s -

g a o a e c

e l l v y f

r r h i e r d f e e p s e r n g l c p S e e o l

t e n y r o c k o

o y c e l r y u a

o e

i e e p l a s t

n e x p x a g g e c

t g s m s t . . n i p r a e m e s t i F t i r n a e

e t l m h m n i s s p e l h t

i l t . p i n e i d l o

e o t o . p f i l w w T o a o u p a

e 4 t n n r i e

i u v s

e e e i t r e N n r d n o

• b n a n o i x s t c u o

- n t o t r b s . b d z o o e

n o i i r s r r s d e a

i t t

n b

o e r , n

u l o a p o i a o , C c a i t h h e t y o t s t

a t r t v a . s l

n m h r r

e a y l

m l r d s i g n w l l l y n t n

e l

x p t t i a i o t . e

a n a o l i

s c v g e r

a a h , y i n i k

h l o t - a m e

p n • • • • b y o

m u r i n s

p h a s e e n i y i s - t d w

e i i d o n r

n h d l g n l o n s r . i p n p

s m d h e l p t

s p l u n p i

e a e a l

t e o

h m m p

h a I i f e t

u e h e

p i I m h a r e n e s d n

h S o r n n i s p s I i r e i t r h r e t r t m t c c n i o I i o o , o

n e r o c

o i i f o n s e a f a n y d

v h . e

a o c n v e 3 y l h s

a z m P r x i s u • • • • • • • • d m i t i m n T c g o d t t

f o e e e u m t s o g s g t . e i

y i i L a n i i h p o e e

c y v v l c a - . c u

r u e l t o

• • • • • n l m a

l i b m e c F

l c d r o y r h o i n n a t t e d e a n

p s a n s s o t o e e

s c T r a e c m A

b n y o . , o

t c o

e a t

n o . m

c m

h p

g t t t - e e s n b l A i p t i M c r e e s r o r f - l c t c s n y n b d f r s e

r u y y i a a 1 s l s d

o i i i i 3 l t o

s i o x o e

t b c e d g g . r

i . t h s p l i m s l e n f n ,

t n a • • h n n a

a i 9 o d r

t l T

T y u s k l n n t u o e l l

c e a s s u o a e a t s e i t n d . t R d i o - , i t m - a s i r p u i r

r p p n i e d

• t m o c

o t c m s r

e T o i n y i k r a

a u f , l g t m t

o o e s e h i a e f n t

i n e a i ,

D l i . C n e t z

i n v c r r

n .

r r . c l h e t n c s o

o l b t a k P

e g r t t a h a d R o e o l e R

f I i

e i s d e f

u i t i l a n o s o , o a o c e l n n

f p l y

i 4 p n l

o k n c

, p r l o

d n c i c s r r n . t c

d T t d

c n r e e

t e i e G

x e t a

y n A i . u l i e o o o o d c c i a r - i i n a l

c r i p s n n l r e t u c t • • • • • • • • • e d

c l y h

h m r o r

n l l u a o e t i

s s t t a p g t

i e a r i g a g r e t o e a a o l i t D f f r c t i

p u h g y a r i r p o

g l

e o s c a t r g s

r n n n l s u o v t i t t e r

o a R i l v e l r i n . e r x

o l h

e a e o

i o n n i m t

c c a t

a e r a w

d e k i P a l 5 r n r e d c v x c u n c e e l e o . n

n o t s i

i l t

t a h

y l l i i n s . • • • • • • • s l l f a l m s e h r a i l t . f t a d b

o • • • • • • • • • l o v e y t o o i e r f f

- t n - x

e s

s u u i l • • • • •

a r n p r r

a p t m t e a r c p m a m d i r u

n n

t b r

c w n e l a t s • • • • • • • • • • • i r e n a

o m y e c o c u e e s t o f d p e x f

d i n t o t t n s c t i

l o e d t i i o c l

i o l o o

s o • • • • d s s . o l

f i i • • • • • • • • • • • • • • • • • k r

n f t u

n n • • • r . i a • •

n a

o i • • • s n m

• • • • • • • • • • • • • • • • • • • • • • • s

s n • • • • • • . g • • • • • • • • •

i . • • • • • • • •

s . • • • • • • • • • • • • . • • • •

Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin Protein

Immunoassay kits CD254 ODF, OPGK, RANKL, TRANCE Activated T-cells, stromal cells and osteoclasts. Plays a role in T- and B-cell as well as T-cell and dendritic cell interactions. • • • • • Involved in osteoclast differentiation and consequently bone resorption. CD256 APRIL, TALL-2, TRDL-1 Leukocytes, pancreatic cells and colon. T- and B-cell proliferation, activates NFkB, and induces cell death. May be • • • • involved in monocyte / macrophage-mediated immunological processes. CD257 BAFF, TALL-1 Monocytes, macrophages, T-cells and dendritic cells. Involved in the stimulation of B- and T-cell function and the regulation of • • • • • • humoral immunity. CD258 TNFRSF14, HVEM-L Activated T-cells, activated monocytes and B-cells. Stimulates T-cell proliferation, receptor for Herpes simplex virus. • • • • CD261 DR4, TRAIL-R1 Activated T-cells and selected tumor cells. Induces apoptosis, promotes activation of NFkB. • • • • • • CD262 DR5, TRAIL-R2 Leukocytes and selected tumor cells. Activated by TNFSF10 / TRAIL and transduces apoptosis signal. • • • • • • CD263 DcR1, TRAIL-R3 Low expression in most cells and negative in most tumor cells. Receptor for TRAIL, competes with TRAIL-R1 and TRAIL-R2 thus inhibiting • • • • • • apoptosis. CD264 DCR2, TRAIL-R4 Low expression in most cells and negative in most tumor cells. Receptor for TRAIL, inhibits apoptosis. • • • CD265 RANK, ODFR Dendritic cells and activated monocytes. Receptor for TRANCE, essential for osteoclastogenesis. Enhances T-cell • • • • • growth and dendritic cell function, plays a role in lymph node organogenesis. CD266 TWEAKR Endothelial cells. Receptor for TWEAK. Weak inducer of apoptosis in some cell types. • • • • • • Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. CD267 TACI Resting B-cells and activated T-cells. Receptor for CD256 and CD257, mediates activation of the transcription • • • • factors NFAT, AP1, and NFkB. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. CD268 BAFF receptor Resting B-cells, lower levels in activated B-cells and resting B-cell receptor for CD257. Promotes the survival of mature B-cells and • • • • CD4+ cells. B-cell response. CD269 BCMA Mature B-cells. Receptor for CD256 and CD257, activates NFkB and JNK. Promotes B-cell • • • survival and plays a role in the regulation of humoral immunity. CD270 HveA, TR2 Widely expressed. Receptor for CD258 and CD272. Plays an important role in Herpes simplex • • • • virus (HSV) pathogenesis and mediates HSV entry into activated human T-cells. CD271 NGF receptor, p75NTR Schwann cells, neurons, bone marrow-derived mesenchymal Regulates neuronal growth, migration, differentiation and cell death during • • • • • stem cells. development of the central and peripheral nervous system. CD272 BTLA CD4+ and CD8+ T-cells, remains expressed on Th1 cells but Lymphocyte inhibitory receptor, regulates lymphocyte activation and / or • • • not Th2 cells. homeostasis as well as T-cell tolerance. CD273 PD-L2, B7-DC Dendritic cells and activated monocytes. Stimulatory on dendritic cells, inhibits T-cell activation upon engaging the • • • PD1 receptor. Mediates T-cell activation through non PD1 mediated interactions. CD274 PD-L1, B7-H1 Activated T- and B-cells, dendritic cells, keratinocytes and Essential for T-cell proliferation and production of interferon gamma and IL10. • • • • • monocytes. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. CD275 B7-H2, B7RP-1 Activated monocytes, macrophages and dendritic cells. Ligand for CD278. Costimulatory signal for T-cell proliferation and cytokine • • • • secretion, induces B-cell proliferation and differentiation. CD276 B7-H3 Monocyte-derived dendritic cells, activated macrophages, Enhances the induction of cytotoxic T-cells and selectively stimulates • • • • • epithelial cells, activated T-, B- and natural killer cells. interferon gamma production in the presence of T-cell receptor signaling. Regulatory role in bone formation. CD277 BTN3A1 T- and B-cells, natural killer cells, monocytes, dendritic cells and T-cell activation. • endothelial cells. CD278 ICOS Activated T-cells. Enhances all basic T-cell responses to foreign antigens including proliferation, • • • • • • secretion of lymphokines, up-regulation of molecules that mediate cell-cell interactions, and effective help for antibody secretion by B-cells. CD279 PD-1 Activated T- and B-cells. Inhibitory cell surface receptor involved in T-cell tolerance. • • • • • CD280 MRC2 Endothelial cells, fibroblasts and macrophages. Endocytotic lectin receptor displaying calcium-dependent lectin activity. • • • Involved in cellular uptake, remodeling and lysosomal degradation of extracellular collagen matrices. May play a role in the plasminogen activation system controlling the extracellular level of PLAUR / PLAU. CD281 TRL1 Monocytes, macrophages, dendritic cells and keratinocytes. Pathogen recognition and activation of innate immunity, regulates TLR2 function. • • • • CD282 TLR2 Monocytes, granulocytes, macrophages, dendritic cells, Mediator of innate immunity against bacterial cell wall lipoproteins. Induces • • • • • • • • keratinocytes and epithelial cells. activation of NFkB. CD283 TLR3 Dendritic cells, fibroblasts, epithelial cells, glial cells and neurons. Mediator of innate immunity against viral infection, activated by dsDNA. • • • • • • • CD284 TLR4 Monocytes, macrophages, dendritic cells and T-cell subsets. Mediator to innate immunity against bacterial polysaccharides. Induces • • • • • • activation of NFkB and production of type I interferons.

Discover more at abcam.com/CD Key : Featured product C n T C C C C C C C C C C C C C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D D D D D D r m g 2 2 2 2 2 2 2 3 2 2 2 w 3 3 3 3 2 3 3 3 3 3 3 3 3 e e 8 9 9 9 9 8 8 0 9 9 9 9 0 0 0 0 0 0 0 0 0 0 0 0 2 t 8 8 6 4 0 9 6 0 5 2 9 7 6 2 0 0 0 0 5 4 0 1 3 0

a g c e b f d 3

T n A A A G T T T C L B C B A L C C C C L N C C C C a E A A T L L L L R l M M R L L L L L L e L L L L P t m e R R R R P P I I M E M M M M u M E E M T T R R R P P r r 1 8 1 9 6 - e C C C 1 4 n ------o 4 R R R 2 1 B 8 9 6 2 7 0 1 4

a 4 1 4 p 4

- - , , , , ,

3 t

1 1 M 0 C

i i O l T I I I

v i R R R A B A

n , e , R

, B E E E

E - A

- C M M M R

S M L D - 3 - I K

2 1 4 G A

3 N - 2 , ,

D K C R - S 5

I G N R

c m m m M c e e m N e N C T N B P L M H M S E M M E M M B T M D M B P d e e n p x i - e h - e k r n a a l e e e a e e v a a a o e o e o o o o o a

a c y l l p d i n a 2 e r d s s l l e u u l t m n t n c c c s n s n n n n n c s s s e t l u i h r o d u n

l o o h p o r t r d r r r e e r e e m

, o o o o o o o c l r r

e a o

a o o o o t r l l d p p r h t a r o a s a n n g e s t h c c c c c c c i h o l t n i a t n p p p p a

h h e i t

n o s p l r y c y c y y y y i y r l e B a e c

i a c l a l

d h h h h c a c i i r s k i d

t h t p h t t t t t h l l l l y o

l a - n m e e e e e e e s i s l y

a a a a n i , i c a

y o y i c u l d c t

n l c l , , d l s s s s s s s t l e g g g g b u e s

y e e

m m o e u i e m e b

e m m , , ,

, e , ,

s , e e e e a m l c l

r l d i l s l o l

r l l m C m n m l m d l t l a a

s , c s o e . l s s s s s s m s n i c a a c e

s c

c p e

. l l m a , . . .

. o o l D , l d

e a a a a d s s . n

y l a

e a h c c u s n r

c T p d t t e l c c c c

t d 1 o n d

e e l . l

T t e a e s - c

n c c r r r r r n 1 r n d m i l c l l - o o o o s o , a t e l e i l l e i e o d c l c t s s

u l o e

p p p p s i T p c u l i i l l + a c d e . , r l m r c l l c l , s

h h h h

s - s h i l k a

a n b e t l

s a r c y h c l , i d , , a a a a o i m o s l c

n d

l n e t

e e b s

e e e e e g g g g k c . v

n d

n d l c a l , a o

o l m o n n i y s e e e e l e

l e s

e p t l s r n t s e s , d d s s s s s e s u . t e u

l u e , e i i m k o l p o ,

r s r r n n

s s r

a a a n i

b r e l e

e d t s o r t c i o

o i

p i a h n n n o v o p s a a

c l i l e n e e p n p d

r e c e d d d c g e h

n e n o n s l c t h o h e r y l r l

e t e a d n d s i

, g o d d m m e s a i p i n t a

n l l r , l

d e

k e p r e , s s l

i l a p h d n l

y a i m m

i d i o s s , , t B n h t n c d l l i r

d i e o s

a l e . , c t n n i i a e d s u b m n -

t h t

l t r s

p n i c o o e e e

. g l r

c s l c e e l c o c m i e a l d s i

r u u n t e

c e a d y e l

e i c o . l i t r c c t t d l s l a l s

a u

l i e r r e s l l d a d l t

e s l , o o o m s l c i s r c ,

n e

e l

c l b c . t p p T y , e s l a d h

a s

n e

e a c t h h - c . l n m e o e , l c

d l e l s s

h i i a p l l d n l l c i l y s s r s s . o d o i l o n a a l

i h d t . .

s , , t n n

p o n

d n

i l p o r , c

m m e o h i i

d c

c d t r n

m B u i c i e c e r r c o o l

d o s e p r y e - - l o

n n o l r c d

c c a t s r l o a n e o o l e e n e y e s . t c n o i - c c

- l s t l . c n l d l y e d d c s y y s

d e t c s e y . t t

e e e r

r e

t n m i s a e i s s t v l d i l

n s c

s a e a a a r

d . i s d n a c n n t

i t e

d n c d d

p t o a d a a d M M h f a t i v a F b I C C P R u R T P R P i R R P f a I M F R P I A A R P A R T p n n h o h n u n n i e p c u n a r s n e s n c - l u u L a a a l r c c l n r a e o o e e e e e e f

a r r e h n h h Y Y a o a a c e a l l t t

t v n o m d c m d g o

n R t t a i r t r t t g m l c g c c g g e w i i s

i c i i e n y v t y i s y a e h i v v D D e t t v l b b b t c i v p

r e u g c

m e e e i i u m n u u u e o t 6

e s b u

s l o a e a a c c i l s e

o e t l i a i i i p l m a y t t t i g 8 8 e o i i o t t t s p p p l f l l l

r c

n : o y r i o a p i i n r n t s n a t o s o l a g n a a m l a z o a t a o p p T . i s h p l h 8 8 n i m . a n t t t l a i

- e R o t s r

u a e r n r o o o t e t t t c i n

n R a a a i p t m L c y e c M e

- m e s e i n

y y a s r

a c r o d n y g a a o l a s c s o g r r r n y N a s e o t t s o y l e a a R s

s s e

t a

n e e r p i , e

t a l a n n n i a t r r l n m r f

- o f f f

n a t n n

l y l t o l t

o A

i e c e o o o n e e e o t r l

2 I s s h h c i n l f h m i p V p d d

y n a u r e

d c o i F t y e t m s o o l i o n e c c

a r r r

e m y y

e i t a d o

o t

a t l a o m i i i i d l E

n d o i c , N r m T T u f a m n e n g n e n

n u p h n t

t l B B u f

m t

m p m e t o n r o p c t u L o

. e i f e d t G r r d R R

p u

r o i r m - e c p e r

h M M e e b t t t t c e u i

p k d r r a a n a - N g g i s e u n o o h h h a a t i t r y i n

t h s y o s F A A e z e g n d i l i o o n t n k o a

e l l g n

a l t n c n n g P P t s e e e t a p c i p b

e o t t e o

n l i r

i - i k i r r e F F r s d g l r e a h i y l l d a o e i d n e r e t

h a e + i t

o a o

e - 7 l p s s s r a n i i . f i i m k e t e

i i w t a e 6 6 i l e 2 l v i t n n n x r e f a

y o

o a r t s h n

- n c ,

v l s n

o f e s I i a n d i i c e z c e

c d n a K , , a m

e l

t r n n n . o s h s , n

e t i a p

t l s

e e a i e r

o r n d u a y t e e o r b r e c l l h e n c r t i i r e o v a a e a n + i i

u e e t s e t o

i n i - x e a n c c n m e g r d

c n r a g r e o o

c s e s d o p f s

t t c c . p n

e s a a p . c

i n p h h r n

n d a - e e e s n d p

a e b g i p r n c A i s

e l e h t s d o

i e s d e c d d l s d r v i G t

a n n s

n s d

a a

i u e l l o e b t

i i m e B D t p . o t i i T

. l e s e p o a i l e o i i a e i d

i m m n s i o i f R n l

n n n y m l n l r i o D o v o s s l t a s a l i t p p t t N n M . r P n d

s a , s a d s o s s g i i d i g g a p i n

. s

n N n o p m m o d n n a e t F , p d

a , m m e

y -

F r . o

h t i t P i M

a m h n r e . g

o h t t n c

n h A e

o 5 B

y n a

u u

i - f o o a M r o i c

b N m

a -

o e i n u a n

d e

f . a a d n n c o . o o o n l - n n 4 g

t i i m n o

o o t r l n n

N N h l

m f Y e

- o P n o y

d d l f e a g p f b i o e e . a o l

n - l s t t a

t v e m i n n

t a d o n i c D

F F i - c l t h e i P n

e n f a e a r a h t n a t l a d o o m r r g

t e

r i c e y o

h s a n k k k r c d r e e z e y

n o t 8 c e l t i d y c i r l u a f t a l r e b e u t n h l d e v B B

e e e i y y o r m e

s s x t l

i m 8 o r s t u n s

T a o t

y c S g u s r e i g e u

d l s t s s p p u l a v

p e C o i c l g r r o s m - t n a

a a a n u t

a b d

i c A i i a

e o o i a t p s s c e s t h i d s c g n o

i o p i o t D

n c c f l i t n r

d i a v c

s e n n t i a o r u r R r , l

i n i a o n a o a n o s l h N i t t a o s n t

t o o e d e e

i o

p 3 i i l s s t n r o d o g t g w a s c l c i r v v l

S n o i m i n

i f l

t r g

l A s

n w n o o e e o 0 n e l

a i m T e o k e a a n l a u . c o w o

c n t

i . n e

n n

D l • • 0 n f o

h m e c n

c l s i

R t t e c e o y l t t o n l i l t f - b e a a a i i e

a o o n s s

o g h

e f e a r o o d r e + t g

( f o m

m I

A a l r i m l l e c e i e

C b F o p l r

i c n n r n l

a a i

m m c f n T a c d l o t t m n t i a

g s F

y u o o r c p h t o N o i , ,

r p b h n t f m o g v n i o o Y n

a s n , u i

o a h i i c 6 l l d a e c c

r v a

G p r c c o a d e a - -

d a w - a p i y a l l R

a t i a , a r y y a s

n r e r t a i r r l n l d c

c t

g r

n e i r a

t v a o ) t o o m m n i t t l

n t r n t c l d g

r m i O

t e e o u

e r o o c p i x o r i i r f c c s i e l p d m b b e d o r e - g o i l

u o e

l n l a a

e a n k k a e h e r m u r i l B e e s i t i d i i s m e g l s

n y m c e u n g a a o

i i d l l u i l t s m a a p s p s l n n n n u c e b . P a . s e

e a

g p u u l l s t i l a . c

m n • • • • t o

. i o m e t e e n l a p

y .

b / n u m o s c a a a i u

c s l c i d a

• • s d f a n

a n o

g t o b y

e m u r

g g r l

r e l u G s s o h T s

m a a f t

e s n s

t a l e T s n o o e e a e e r l H n t r e o i c o h . l i e t t s r s t o e y t n i s t - i o e r a r n n c c c i s a i a i s i o a i t 2 I g c m . t d

o y

c d . d i V t r r i r y

s t t p . N m d m

o d n

e i

e s

s s

e e n y o • • • • • • u I . v e

t c r d

- r e n n

e a

l . . F

n a

t t o A g 1 e e o a

l e o x c

c b e i i

s p h A A s d r o o . n a k l i n , s s l t p e c l y f e y v n n • e

i o , l i

i n n B c c n

d n a e , b H i

p t t r l c

i d t t

c i l i s o o n e t t

o d e i t

g n v n i

y o o s i i a a I r t u

r i s v k l

V s n a a t r i o

d t e t l p c

m n t n n i l a a a o t a i v s v i

c t n - e s

q y r c • •

d d c e n t c l 2 i t i o e i • • • • s t i y o a o e t i

g u e n y s o

d t c e c , t

. t t i b r

n s

e i d t

• • • • • • • o o i h e e n

a o c v • • • • • •

l b

e i

• • • a n p e l

t i

b i • • • • • • • • • • • • l l i n t y a y s d • • • • • • • • o s , h o s

. t

t n . i

s • • • • • • • • • • • • • • • • • • • • • • • • • • l c . • • • • • • • •

. • •

• • • • • • • • • • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome

Biotin Protein

Immunoassay kits CD307a FcRL1 Mature B-cell subsets. May function in B-cells activation and differentiation. • • CD307a FcRL1 Mature B-cell subsets. May function in B-cells activation and differentiation. • • CD307b FcRL2 Mature B-cell subsets. Regulatory role in normal and neoplastic B cell development. • CD307c FcRL3 Mature B-cell subsets. Interacts with PTPN6, PTPN11, SYK and ZAP-70. • CD307d FcRL4, IRTA1 Memory and monocytoid B-cells. Putative inhibitor of B-cell receptor signaling. • CD307e FcRL5 Mature and memory B-cells. May be involved in B-cell development and differentiation. • CD309 VEGFR-2 Endothelial cells and primitive stem cells. Activation of this receptor by VEGF plays a role in angiogenesis, • • • • • development, wound healing, cell proliferation, migration and differentiation. CD312 EMR2 Macrophages, activated monocytes, dendritic cells, granulocytes, Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, • hepatocytes and lung cells. promotes the release of inflammatory cytokines, including IL8 and TNF. CD314 NKG2D, KLRK1 Natural killer cells, T-cell subsets and dendritic cell subsets. Receptor for the recognition of MHC class I HLA-E molecules by natural • • • • • • • • killer cells and some cytotoxic T-cells. CD315 PTGFRN Megakaryocytes, hepatocytes, epithelial cells and endothelial cells. Inhibits binding of prostaglandin F2-alpha (PGF2-alpha) to its specific • FP receptor. CD316 IGSF8, EWI-2, KCT-4 B-cells, T-cells and natural killer cells. Negative regulator of cell motility. May regulate neurite outgrowth and • • maintenance of the neural network in the brain. CD317 BST2 T-cells, monocytes, natural killer cells and dendritic cells. IFN-induced antiviral host restriction factor which efficiently blocks the • • • release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. CD318 CDCP1, SIMA135 Hematopoietic stem cells and epithelial cells. May be involved in cell adhesion and cell matrix association. Potential role • • • in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. Possible marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. CD319 SLAMF7, CRACC Natural killer cells and activated B-cells. Mediates natural killer cell activation and may play a role in lymphocyte adhesion. • • • • CD320 FDC-SM-8D6, TCbIR Follicular dendritic cells. Augments the proliferation of plasma cell precursors generated by IL10. • Receptor for the cellular uptake of transcobalamin bound cobalamin. CD321 JAM-A, JAM-1, PAM-1, F11R Epithelial cells, endothelial cells, leukocytes, platelets and Plays a role in epithelial tight junction formation, regulation of leukocyte • • • • erythrocytes. migration, epithelial barrier maintenance and platelet activation. CD322 JAM-B, JAM-2, VE-JAM High endothelial cells. May play a role in lymphocyte homing to secondary lymphoid organs. • • • CD324 E-Cadherin, CDH1 Epithelial cells, keratinocytes, trophoblasts and platelets. Calcium-dependent cell adhesion protein. Involved in cell-cell and cell-matrix • • • • • • • adhesion, tumor suppression, cell growth and differentiation. CD325 N-Cadherin, Cadherin-2, CDH2 Neurons, striated and cardiac muscle cells, fibroblasts, epithelial May be involved in neuronal recognition mechanisms. In hippocampal • • • • • • cells, pancreatic cells and hepatocytes. neurons, may regulate dendritic spine density. CD326 Ep-CAM, EGP314 Embryonic stem cells, epithelial cells and adenocarcinomas. May act as a physical homophilic interaction molecule between intestinal • • • • • • • epithelial cells and intraepithelial lymphocytes at the mucosal epithelium for providing an immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. CD327 Siglec-6, OB-BP1 B-cells, placenta trophoblastic cells and granulocytes. Adhesion molecule that mediates sialic-acid dependent binding to cells. • CD328 Siglec-7, AIRM-1 Natural killer cells, monocytes and granulocytes. Adhesion molecule that mediates sialic-acid dependent binding to cells. • • Mediates inhibition of natural killer cell cytotoxicity. May play a role in hematopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells ( in vitro ). CD329 Siglec-8, SAF-2 Eosinophils. Adhesion molecule that mediates sialic-acid dependent binding to cells. • • CD331 FGFR1, BFGFR, FLT-2 Epithelial cells, endothelial cells, fibroblasts, mesenchymal cells Cell-surface receptor for fibroblast growth factors. Plays an essential role in • • • and cardiomyocytes. Also expressed in astrocytoma, the regulation of embryonic development, cell proliferation, differentiation neuroblastoma and adrenal cortex cell lines. and migration. CD332 FGFR2, KGFR Neurons, hepatocytes, fibroblasts and epithelial cells. Cell-surface receptor for fibroblast growth factors. Plays an essential role in • • • the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. CD333 FGFR3 Brain, kidney and adult testes. Cell-surface receptor for fibroblast growth factors. Plays an essential role in • • • the regulation of cell proliferation, differentiation and apoptosis, as well as in chondrocyte differentiation, proliferation and apoptosis. Required for normal skeleton development. CD334 FGFR4 Liver, kidney, lung, pancreatic cells, lymphocytes and Cell-surface receptor for fibroblast growth factors. Plays a role in the regulation • • • macrophages. of cell proliferation, differentiation and migration, as well as in the regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis.

Discover more at abcam.com/CD C C C C C C C C n T C C C C C C C C C C C C

a a D D D D D D D D D D D D D D D D D D D D r m g 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 e e 4 5 3 3 5 5 3 5 3 3 4 4 6 6 5 5 5 5 6 6 t 9 2 9 8 3 1 7 0 6 5 0 4 2 0 4 8 5 7 1 3

F N J A S F n A N F N N E F S I T T C T E S L a a r C r r R N N B L l R y V 1 T C C C R - t i i i g m z z z e 2 n A P B A E F F C I B T R R R g 2 z z z r 1 d M R R R e A n - M M G B l l l e B 3 2 1 R e e e e A

a 1 M S S d , , , F 2 c R - 2 d d d ,

t 1

N N N 1 ,

a F F 8 i - - - S

v 9 1 4

M , n 2 1 K K K e

L , 0 , J - 1 8

- - - L A 2 F F A , p p p

N , F M z z G 3 4 4

H - -

z 0 4 6 1 9 4 F 1 - S

9 , 1 , 6

P F F 0

G , Z z

E F D ,

Z 4 S 9 D

1 C 0 2

c M a M N e p p H N T P e C S A N B s E U H s L T C L T B E l l l a e y e e t t y y - n x l l x - n h - l c c o p n m a e a e a b e a r e a a u u e e c a c c m m l t p i d d h t y n i d l a t p t l t i p r r m c c k k e e s s e i c t s l q u u u p d r v a m

u h w e o e e o o t a a p p e a e e l l E m , l u e r r r h a l l l

i e

l e s s

t o n n c c t t h h c a a a a n n a n n s o s i m p h d o o o t m e , t l . y y e m t t e o o l l s l r i c g n e t

o c u s e

a a

a c c c b a

l t t k k k a m l i d h i c a y i n u b u e e b t l o

y y y l d l l y a o ,

e i i i i

a r y

t y n

s l l l r r s s s s c t t t a n o

u n e r l l l l n f c o y e e e i t

t e e e n m u e o o

. l e e e n c i c s a

s e e e a o

s n s s s r r r d c b t w c - t l e y s d c , a

t l a l x n s s n , , . B s e

l m s

c c c u y s

l s l

s h

l p u , l i l l d e , e i a . e

e e e l s t

a e n s u r

, p l s e k e a s r e a

t s

s n t n l l l .

r t t n e k e P c t l l l l r i s r p t a s e ,

r s l , s s s e d e d d o i r

e g e

s s . u , l k r , v o s , . . n v n n m

o m

t r s

t l s p f i r a t p

l T w e i c

e a o l e s e n r i t i k c l u e n

o h s e a a e u - s u t , c , y c t o i e

e i

n r a a c n e

l l r r n s , a p y r e o l

i c a l

s s o p u j l l c l . - l f o t e i c n e

s l v

y i

c n e t e . l , e a m e n h l H s e r r c

d . a a

u d t s a s i c e p l l s

e , p y a l

l r r i e

l l

s c g e n B , s i a s y

r n t h a k l t

s s e

a , e l h h l s , p .

r - e l e e i

l n

a m

o u d s l t s l c l f t e e l y

h l u r n d o a h n s d . n b n , e a l s

y r o i

r c y

t , d e o g l e a l l a b t h

m o

l o m c e m

k s b

r v

y l e l o y f o

t a m y

n e . . l e e a c h i p o

x n m o d c t n

t r e t d r e

r a p o a e c a

m i d e u e l a l

s l r d y l l

e t l l

s c e m

y s i p n

c u f c t

n k u a e , m e u m

s i e , e

s i a o t a k

n k t d s r s h p l c a l o s n c o p l r l i d , n i s e s d l r r o

l n o y e d c h

p o o e

, l n b p n o a t i

r y

o u e s w e y p c s s e r e

n n t b . t i a m r s e a e , p y n

a r u d i

l c , p i i s m , n l l a

n t p

c l e ,

e a e s h h .

l c s

l . u h e e a e

l , d , e e r t l

n e s n n o e m a f p u m r i . g r d . b a i r a a l d a

m t

a o r l c t

n a

l i o c

b c e t c t a c b n o h a b e

l l r r l o c l d p e r s l o y e l a d l e o

, o l

o a

i s l i e a l d f

i i a l d i n e t t s b p l i

s c

e t

r c i

i a

t , o c s c e h

n b

. e l e

l d l

s l a l l

i s , a s

, t l

p a r c a R t t d r d i c a o e R T C F x C o L d C t M s X R t R P c C R S I R M a i R T I P i i o n n n n r h r r n F e e e r e i o f e i c f f r n n u i e c r r a a a u e r t o g e e y y y e e e e e e f g v c d f

i r t a a s o c N i i i o f t d i t n l m t i a e g g n t v d d n o n n m n t t t n s n l n o r e c i c c c c l c c c i a i u p e g y y c - c o o o l v v t i e o e - c r g g s

s u s s

c s i m f e e e e e o e e f e a l r n c

o e p g r a i s a e a u a a v f l t t t t s a e p a i b l m m m e e t i a g o e o o o p p p p p b p p i t o l i i d b u l i n s t a x c e n l l t n i t b v e i

e o s n l c i

o a r r n m h w e e e r x x x a t t t t t i t t a o p s o

l r r t i i i s m d c s s a o

l o o o o o o o e d y e x t s s s n f t i r t o f g e i i i

d t s d

o y e i t m o i e t n c c c e y

a m - t

t y t v

c e r r r r r r r a n s s s i i d s h c r l

h , i

m e i a m

c i

i i i r

e n

a s c r c o

n e e a T n r i i i a t t t s t t f f f f f f f

e o y t

c o o o s e a o d t y y y u

e i o o o o o o o s

o a

e m

l f v p l t a a - e a . e t t w

n t n f T i e e

n n n e

t - - - s

r h u c c d o n r r r r r r p r r

f o n o B i n E t

d t a a a d o a

m e

t - p r a l t a u i

e s e i u i a r

l r T W t W W i t . n h d l c o a a t r o o o N a y

c c c n i t r l - f h h v s c u u r n

s h l e a y d l f e r r t i a e t c e e i e

N l o m l t t f f f s a t t t t

e t a e s t c e i e s e

F s t n s

c i

i i i u n n n t

r p e i e c t m r l c

l r v v v C s p p p t e v t t i e r e j l d

l F t i i

k i r o e n e r k

i e e

r t t t

a e n l r a o l a n

F l o a a a u s a e o t o o e a k

d d l n a y g

a i l A o S e B p s h

p p p

c n e l

a i n b b g s f t t t e c B r i t t l l l

l s n

i e t l u c

i i i l r p a a a d

h e i f e t g D c e N

r r r c F

i a u l n n n l u d e i . p

o s f c t i o a k

e e

a o o o l x t - l f o o e r r r i e e e i d

l i a c m a i s k 1 g g g e

r l a s . r y M l n o y z i i i i b s l v p r t n i t t t

e l n a t t t n

r o r l e l

n n e e i e e e t

l a

t s t c a l y y y c l

t p i n n e r

e r e e d c

m r r r o

n o t b s g

c 1 - p t e g r a

G i i i e . d a n .

o y e e e t o h h - a n i n n n e r f

i i i e t d h r i y n m

t a

a o 2 i n n n i t p

o s

S o e n d e m c c c

f p i o n o a t t s s s l - h c o s

n o

f l u i n g r 1 s f f f r p n e e e i x a

d C r s t a l r e e . . , g . r t e o o o n e

m n t m o a t u g p e f

o i r o t e . i r h

p p p t i h

o t M M m e M m n e c h l • • • • • • a D r r r a

a o l t h m m o s m o o i c

l m c y d o m m m i a o t t t o e s n i m t n a n c l t t f o o o e . t e t l n 8 a a a

a a i i e

t i n y e n o l m o

o k i o o f t e o g p p i m t

a a a r r r p p n t o e + f y y y

s y y a d o

b . i o t

e i r e a f n m s t

i r n e l

t t t t e t t t t

f n

s d

e l p l

e R e e i i i i h h h o n c b b b

t I b p r

r i e c

o o o c b m - r

s a c o t g

t c i

e e m s a a y d c c i a a a a r a o n e e e e

e e l r s

e n n n p y . e a e n o

A s

s n

r t e o e r i p t t t t b r

p l

n t c

q i e a

t l l

. y s u l h i i i d f h i r y t

I t d d d l s p

u l d n n n c m m p n

e h e m a i a

g i s u - t o e

I a r g

e a o a

e u u u o t a p h m i n v v v r v p c k

i a l t

n A i i a o f r n a a g l

i m e n t c u a r e c

r r r

o o o n s o n l e n h v a e n t i l d e e t t s

r i i i o e g

o e y y k s t

r n n u e n n n

a o l l l t l

p v e s l k r e i v a e v v v

a d t v d

r i

. v n d s o r s a d I t o m m

g g g m n o . l i c c a m i i u e t e e e e g

v v l l

b a t i i

i s t

o l

f u

n r s o o

r d a l r o e i r d k e e d d d i d M o v m t t t p u o e i t o n i o a n p e n . c m i i i g n n n i

t f r i

e s s s r d

r o I e t r c l

k b n

r o i i i M g i d a l . e

e g

e t t a e

n n n t s s s n c e d

o n

C e b o r r r d c i c h y

i i u M

l n d

M s i i t s n u u u e n e

/ a t

b b p 1 o e r y

t t t e b c A i

l u

e i

i b

u d e e e r r r l n

s i o a c n y t a t u u w t - t l l . c n n e t

a a a r o l b s e o h s D a p i l o

h r

a r t f a y e t t s f t - m m m i l l n n n e a

e e l

e e r a

c n l

h y t e r a e l i M

t a

m d

r n l r h l n s s s e g c f l h l a

s t l

t s

m k t o o o o o

m x e u t e n i t t e o c e e m d d d t l - f c u u u e n 3 i o o o e a y s l i r r r p f s t s n r d y . r e v r a l u u u e e p p p o m l r m

d r s • • p i s m a a . e r . a t c i i a t t f

s y c c c p r n n h h h o - e i h h a c

u i h c t d t s l e a b n o c l n . t

t t t

a i i t c

y o o o t s e e c

e b l o a t i o i i a . o i v c o a A n a t l

r o o o a i a l r t s o e g g g o t o

n t n n i e o r e x r t h t i i J c n n n t c v c e i t n n o

e e e i r d n u n

d i l a k i o a c r N e e e o t y o

i l o

c i c c d n n n n e s i s s a n a a r g (

i o t n s o

l l . n i n f a f S K i r r l l

. r

t e e e e v c n

n n u

e m

e e e a v b y e t T e h

l l b o o e 1 x s s s

y o n d d d n d a

a a f s e a k s - s i o a f f p c i i i l p P s g e g f

. c s s s v

l s s i p t n ,

i i i u d • • • • e l l a s n l i w t n n n

h N

a e u l

. e ) c e s e e o o a d e i a a

i x . y f n d m t t t

l g l P u . s d f x

e d d o

r e e e n l s s

r . n n

T i

i b

i c n

l , d

n e a

t u o m r r r s a • • • • • • • • • • • •

d d i a h e w c i e • • • • • • c c c n a r e f n m e e r n r e

e y h i f e e e f u

l / / a s e f

n s • • • • •

a i t s

c g i

n p • • • • • • o l l l h n

o o c t l . l l l c • • • c

t l h u u u u

g l • • • • • •

f a i i e e r r i y o • • • e e a

l l l l

a a a i a i

• • • • n

n s e n o

r r r t • • •

s c r

• • • • • • y s • • • • • •

• • Polyclonal

Unconjugated

CF™405M

OC 515™

FITC

PE M o n

APC o c l o

PE/Cy™5 n a l PerCP

APC/Cy™7

PE/Cy™7

Other fluorochrome Biotin

Protein

Immunoassay kits Flow cytometry antibodies and kits

With Abcam’s extensive range of conjugated antibodies and kits for flow cytometry ...

...designing your multicolor experiment has never been easier!

Discover more at abcam.com /flow-cytometry

Discover more at abcam.com D D i i s s c c o o v v e e r r

m m o o r r e e a a t t

a a b b c c a a m m . . c c o o m m / C D C o p y r i g h t

2 0 1 3

A b c a m ,

A l l

R i g h t s

R e s e r v e d .

T h e

A b c a m l o g o i s a r e g i s t e r e d t r a d e m a r k .

033_13_AKR