CSIRO PUBLISHING Australian Systematic Botany, 2015, 28,1–22 http://dx.doi.org/10.1071/SB14049 Evolutionary relationships of Heimioporus and Boletellus (Boletales), with an emphasis on Australian taxa including new species and new combinations in Aureoboletus, Hemileccinum and Xerocomus Roy E. Halling A,H, Nigel Fechner B, Mitchell Nuhn C, Todd Osmundson D, Kasem Soytong E, David AroraF, Manfred BinderG and David Hibbett C AInstitute of Systematic Botany, The New York Botanical Garden, Bronx, NY 10458-5126, USA. BQueensland Herbarium, Mt Coot-tha Road, Toowong, Brisbane, Qld 4066, Australia. CDepartment of Biology, Clark University, Worcester, MA 01610-1477, USA. DDepartment of Biology, 3034 Cowley Hall, University of Wisconsin – La Crosse, 1725 State Street, La Crosse, WI 54601, USA. EFaculty of Agricultural Technology, King Mongkut’s Institute of Technology, Ladkrabang, Bangkok, Thailand. FPO Box 672, Gualala, CA 95445, USA. GAsam Strasse 50, D-93352 Rohr in Niederbayern, Germany. HCorresponding author. Email:
[email protected] Abstract. Boletellus and Heimioporus, two genera of Boletaceae with ornamented basidiospores, are shown to be distinct genera on the basis of phylogenetic analyses of nuclear ribosomal large-subunit and translation elongation-factor 1a DNA sequences. Comparison of spore ornamentation type – longitudinally ribbed in Boletellus v. punctate, alveolate- reticulate in Heimioporus – are further evidence for distinction. Analyses of multiple accessions from the Americas, Asia and Australia support the monophyly of Heimioporus and a ‘core Boletellus’ clade, containing the type species, B. ananas (M.A.Curt.) Murrill, and approximately seven additional species. Tests of alternative phylogenetic topologies could not reject monophyly of a more inclusive group containing the core Boletellus clade and six other species.