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Home , ACACB, Aldolase B, GLUD2, HK3, PFKFB2

Supplementary Table 2 Effect of each siRNA SMARTpool on caspase activity/cell mass in each cell line/medium condition, expressed as a z-score.

RWPE1 DU145 DU145 LNCaP LNCaP PC3 PC3 Name GeneID Gene Symbol (KGM) (FM) (LDM) (FM) (LDM) (FM) (LDM) 1 2584 GALK1 2.54 1.15 1.50 -0.19 -0.95 2.70 1.85 galactokinase 2 2585 GALK2 4.76 -0.17 1.03 -0.14 -0.31 0.88 0.71 galactose-1-phosphate uridylyltransferase 2592 GALT 1.98 2.12 0.95 0.21 0.00 0.11 0.94 phosphoglucomutase 1 5236 PGM1 3.02 -0.11 0.06 -0.98 -0.76 -0.73 -0.02 glutaminase 2744 GLS -0.88 0.08 0.09 1.69 1.03 1.99 0.45 glutaminase 2 27165 GLS2 0.42 2.31 0.18 0.66 0.21 1.27 0.12 glutamate 1 2746 GLUD1 2.73 0.11 -0.38 -0.04 0.13 -0.70 -0.64 2 2747 GLUD2 1.40 0.08 -0.14 1.30 -0.33 -0.62 -0.32 and uptake solute carrier family 2 (facilitated glucose transporter), member 1 6513 SLC2A1 -0.55 -0.30 1.17 -1.34 -0.89 -0.26 -0.96 solute carrier family 2 (facilitated glucose transporter), member 4 6517 SLC2A4 0.92 0.88 -0.26 2.75 -0.27 -0.19 -0.07 1 3098 HK1 -1.29 1.00 1.57 -0.62 -0.38 0.31 1.26 hexokinase 2 3099 HK2 -0.83 0.36 -0.68 -0.88 0.17 -0.68 -1.24 hexokinase 3 3101 HK3 0.77 -0.67 0.04 1.10 -0.63 -2.18 -0.68 hexokinase 4 2645 HK4 -0.58 1.06 2.47 -0.05 0.59 -0.12 -0.01 glucose-6-phosphate 2821 GPI 2.38 0.69 -0.18 1.20 0.20 -0.28 0.33 , 5211 PFKL -0.38 1.60 5.06 1.86 -0.23 1.54 1.16 6-phosphofructokinase, muscle 5213 PFKM 0.53 -0.65 -0.46 -1.17 -0.85 -1.63 0.00 6-phosphofructokinase, platelet 5214 PFKP 1.91 1.76 0.74 0.88 -0.24 0.25 0.58 6-phosphofructo-2-kinase/-2,6-biphosphatase 1 5207 PFKFB1 2.67 2.96 0.90 1.16 2.81 3.08 1.26 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 5208 PFKFB2 -0.01 -0.07 -0.76 0.49 -0.75 0.38 -0.04 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 5209 PFKFB3 1.42 0.94 0.18 0.18 0.87 -0.27 -0.85 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 5210 PFKFB4 1.36 1.48 2.87 1.09 2.92 2.67 3.02 TP53-induced glycolysis and regulator 57103 C12orf5 -1.24 -1.15 -0.92 -0.50 -0.92 -1.06 -0.60 226 ALDOA 0.79 0.47 -0.53 1.09 -0.02 14.83 1.46 229 ALDOB 1.43 0.52 0.46 0.83 -1.11 0.14 -0.46 230 ALDOC 0.54 0.10 0.21 6.64 0.08 -0.93 0.15 triose phosphate isomerase 7167 TPI1 0.33 -0.49 -0.48 1.98 -1.04 -1.76 -0.89 -3-phosphate dehydrogenase 2597 GAPDH -0.13 -0.68 -0.58 -1.39 -1.15 -0.56 0.45 glyceraldehyde-3-phosphate dehydrogenase, spermatogenic 26330 GAPDHS 0.21 -1.45 -1.55 -1.57 -1.24 -0.61 -1.30 1 5230 PGK1 -0.73 1.36 6.94 2.49 0.32 -0.71 -0.86 phosphoglycerate kinase 2 5232 PGK2 -1.07 -1.58 -0.83 -0.14 0.55 -1.04 -0.25 family member 4 441531 PGAM4 -0.30 -0.74 -0.53 -0.38 -1.41 -0.43 0.15 phosphoglycerate mutase 1 5223 PGAM1 -0.46 -1.14 -0.12 0.34 1.95 -0.51 0.12 phosphoglycerate mutase 2 5224 PGAM2 -0.77 -0.35 0.73 -0.74 -0.75 0.25 -0.44 2,3-bisphosphoglycerate mutase 669 BPGM 0.16 -0.01 0.61 -0.40 -0.53 0.47 -0.48 1 2023 ENO1 -1.26 -1.53 -0.22 -0.39 -0.50 0.13 3.34 enolase 2 2026 ENO2 0.15 0.66 -0.05 -0.40 -1.08 -0.37 -0.47 enolase 3 2027 ENO3 -0.49 1.15 0.05 0.08 -0.72 0.79 -0.86 , liver and RBC 5313 PKLR 0.43 10.58 17.64 2.08 1.93 2.23 2.18 pyruvate kinase, muscle 5315 PKM2 0.27 0.36 0.14 0.34 1.13 2.22 6.44 A 3939 LDHA -0.28 -1.19 -1.44 -1.09 -0.58 -0.80 -0.75 lactate dehydrogenase B 3945 LDHB -0.12 -1.06 -1.44 -1.31 -0.67 -0.30 -0.07 lactate dehydrogenase C 3948 LDHC 0.30 0.12 -0.97 0.63 1.06 -0.21 -0.64 lactate dehydrogenase A-like 6A 160287 LDHAL6A -0.57 -0.12 0.63 -0.46 -0.96 -0.53 1.34 lactate dehydrogenase A-like 6B 92483 LDHAL6B -0.46 0.20 1.31 7.51 1.36 -0.06 0.40 solute carrier family 16, member 1 (monocarboxylic acid transporter 1) 6566 SLC16A1 -0.93 -0.61 -0.45 0.31 0.31 -0.92 -1.42 solute carrier family 16, member 7 (monocarboxylic acid transporter 2) 9194 SLC16A7 -1.27 -0.52 -0.16 -0.76 -0.15 0.24 -0.40 solute carrier family 16, member 3 (monocarboxylic acid transporter 4) 9123 SLC16A3 2.06 7.10 5.49 1.63 2.99 4.65 1.37 solute carrier family 16, member 8 (monocarboxylic acid transporter 3) 23539 SLC16A8 0.22 0.52 0.67 0.16 0.24 3.47 0.13 solute carrier family 16, member 4 (monocarboxylic acid transporter 5 9122 SLC16A4 -0.79 -0.47 -0.82 0.51 -0.84 0.13 -0.13 TCA cycle pyruvate dehydrogenase (lipoamide) alpha 1 5160 PDHA1 0.22 10.19 3.84 -0.16 -0.71 0.15 -0.10 pyruvate dehydrogenase (lipoamide) alpha 2 5161 PDHA2 0.66 1.07 -0.45 1.25 3.23 -0.07 -0.43 pyruvate dehydrogenase (lipoamide) beta t 5162 PDHB 0.23 1.58 -1.12 -0.44 -0.55 0.23 -1.05 dihydrolipoamide S-acetyltransferase 1737 DLAT -0.51 -0.01 -0.13 1.20 -0.25 1.06 2.70 dihydrolipoamide dehydrogenase 1738 DLD -0.70 0.77 -0.43 2.00 1.09 -0.43 -0.70 pyruvate dehydrogenase kinase, 1 5163 PDK1 -1.44 0.20 0.98 -0.43 -0.32 -0.15 -1.00 pyruvate dehydrogenase kinase, isozyme 2 5164 PDK2 -1.57 -1.46 1.88 -0.96 -1.07 -0.52 -1.31 pyruvate dehydrogenase kinase, isozyme 3 5165 PDK3 0.55 0.16 0.87 3.14 0.83 -0.64 2.63 pyruvate dehydrogenase kinase, isozyme 4 5166 PDK4 -0.68 0.57 1.16 5.60 -0.02 0.53 1.48 pyruvate dehyrogenase phosphatase catalytic subunit 1 54704 PPM2C 0.52 1.03 0.12 0.15 -0.62 -1.64 -0.16 pyruvate dehyrogenase phosphatase catalytic subunit 2 57546 PDP2 -0.28 -0.96 0.42 -0.17 0.55 0.43 2.54 citrate synthase 1431 CS 0.99 -0.12 -0.39 3.64 1.52 3.96 3.43 aconitate hydratase 1, soluble 48 ACO1 -0.23 -0.13 1.57 0.74 1.67 2.75 2.11 aconitase 2, mitochondrial 50 ACO2 0.74 0.45 0.92 0.46 -0.04 1.70 0.39 similar to aconitase 2 441996 LOC441996 1.22 4.46 1.56 1.70 6.99 7.31 1.44 isocitrate dehydrogenase 1 (NADP+), soluble 3417 IDH1 -1.16 -1.00 -0.08 1.16 0.36 0.02 0.31 isocitrate dehydrogenase 2 (NADP+), mitochondrial 3418 IDH2 -0.24 -0.67 2.32 -1.28 -1.02 -0.99 0.31 isocitrate dehydrogenase 3 (NAD+) alpha 3419 IDH3A -0.16 -0.48 -0.72 -0.44 -1.10 1.14 -0.34 isocitrate dehydrogenase 3 (NAD+) beta 3420 IDH3B 0.02 0.38 1.29 1.18 -0.20 2.33 2.10 isocitrate dehydrogenase 3 (NAD+) gamma 3421 IDH3G 0.46 0.90 1.12 2.08 0.87 0.38 0.21 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 4967 OGDH 2.07 3.12 -0.70 -0.47 0.19 2.23 0.39 dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) 1743 DLST 0.55 0.26 1.58 -0.04 -0.41 -1.02 0.14 succinyl-CoA [GDP-forming] subunit beta, mitochondrial pseudogene 283398 LOC283398 -1.15 -0.98 -0.89 0.86 -0.98 -1.05 -0.76 succinate-CoA ligase, GDP-forming, beta subunit 8801 SUCLG2 -0.52 -0.51 -0.60 0.10 0.00 -0.24 0.30 succinate-CoA ligase, alpha subunit 8802 SUCLG1 -0.99 -0.62 0.15 0.14 0.00 1.66 1.37 uccinate-CoA ligase, ADP-forming, beta subunit e 8803 SUCLA2 -0.50 -0.13 0.23 -0.76 -1.15 0.79 0.67 succinate dehydrogenase complex, subunit A 6389 SDHA 0.33 -1.66 0.03 -1.93 -1.12 -0.24 -0.14 succinate dehydrogenase complex, subunit B 6390 SDHB 0.10 -1.37 -1.49 -0.68 -0.36 -0.04 -0.71 succinate dehydrogenase complex, subunit C 6391 SDHC 0.07 -0.82 -1.24 -1.48 -0.71 -0.92 -0.22 succinate dehydrogenase complex, subunit D 6392 SDHD 10.03 11.25 7.33 16.67 11.81 1.10 3.53 fumarate hydratase 2271 FH -0.12 -1.51 -0.84 2.60 -0.32 0.14 0.35 malate dehydrogenase 2, NAD (mitochondrial) 4191 MDH2 0.38 -0.93 1.11 -2.10 -1.37 -0.39 0.28 Anapleurosis and mitochondrial shuttles malate dehydrogenase 1, NAD (soluble) 4190 MDH1 0.90 0.25 -0.63 4.15 2.79 2.76 3.69 aspartate aminotransferase 1 2805 GOT1 -0.95 -0.24 0.67 0.13 -0.90 2.00 0.86 aspartate aminotransferase 2 2806 GOT2 -0.89 -0.16 0.44 -0.16 0.14 0.50 2.52 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 8402 SLC25A11 0.21 1.25 0.34 0.98 2.01 4.15 1.36 -3-phosphate dehydrogenase 1 (soluble) 2819 GPD1 -0.09 -1.09 -0.29 1.78 0.37 0.54 -0.38 glycerol-3-phosphate dehydrogenase 1-like 23171 GPD1L -0.82 0.21 0.78 0.09 -0.65 1.60 0.84 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) 2820 GPD2 1.60 0.00 4.53 -0.16 -0.61 0.86 3.63 5091 PC 1.77 8.17 1.11 0.11 0.15 1.79 1.71 malic 1, NADP(+)-dependent, cytosolic 4199 ME1 0.33 1.31 2.06 1.60 4.35 1.77 1.51 malic enzyme 3, NADP(+)-dependent, cytosolic 10873 ME3 -1.29 0.03 0.36 0.35 -0.23 0.67 0.90 malic enzyme 2, NAD(+)-dependent, mitochondrial 4200 ME2 1.34 4.05 2.59 0.66 -0.44 4.11 4.45 Aminoacid uptake and metabolism glutamine synthetase 2752 GLUL -0.69 -0.41 0.04 0.05 1.49 0.11 -0.32 aminotransferase 2875 GPT -0.88 0.51 -0.36 1.02 3.02 0.53 0.83 2 84706 GPT2 0.86 -0.38 1.75 0.12 2.14 -0.20 -0.51 D-aspartate oxidase 8528 DDO -0.63 -0.96 -0.25 -0.49 -0.02 -1.33 -0.19 ilvB (bacterial acetolactate synthase)-like 10994 ILVBL 3.36 6.72 4.71 5.68 2.61 0.15 0.48 alanine-glyoxylate aminotransferase 189 AGXT -0.04 1.97 0.81 0.18 0.97 0.95 1.05 alanine-glyoxylate aminotransferase 2 64902 AGXT2 1.65 0.44 1.06 0.46 0.18 3.49 1.15 phosphoglycerate dehydrogenase 26227 PHGDH 0.41 0.49 -0.70 -0.23 1.57 1.66 -0.58 phosphoserine aminotransferase 29968 PSAT1 -0.78 -0.69 -0.78 -1.22 -0.28 0.80 -0.27 ASCT2, solute carrier family 1 (neutral transporter), member 5 6510 SLC1A5 -0.01 -1.05 0.33 0.09 0.14 0.61 2.17 LAT1, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 8140 SLC7A5 12.94 1.25 1.25 -0.35 -0.39 0.63 0.28 carbamoyl-phosphate synthase 790 CAD 1.38 -0.52 -1.07 -0.19 -0.62 0.60 -1.53 4953 ODC1 -0.07 -0.18 -0.10 1.53 -0.20 0.71 -1.29 serine hydroxymethyltransferase 1 (soluble) 6470 SHMT1 0.08 -0.38 0.11 1.64 8.20 0.00 -0.44 serine hydroxymethyltransferase 2 (mitochondrial 6472 SHMT2 0.25 -0.28 -0.80 8.39 1.20 -0.50 0.29 Pentose phosphate pathway glucose-6-phosphate dehydrogenase 2539 G6PD 2.76 2.22 4.69 21.58 6.47 0.64 1.87 transketolase 7086 TKT -0.39 -0.14 -1.30 0.25 -0.41 -1.64 -0.98 transketolase-like 1 8277 TKTL1 0.79 0.92 -0.54 1.86 1.78 -0.12 0.32 transketolase-like 2 84076 TKTL2 -0.72 -0.35 -0.50 0.26 0.26 1.36 2.32 6-phosphogluconolactonase 25796 PGLS 0.36 1.68 0.06 -0.14 0.00 -0.53 -0.66 hexose-6-phosphate dehydrogenase 9563 H6PD -0.76 0.45 0.24 0.22 3.46 1.10 0.86 6-phosphogluconate dehydrogenase 5226 PGD -0.28 1.82 -0.13 -0.24 -0.06 0.15 -0.39 ribose 5-phosphate isomerase 22934 RPIA 1.16 0.00 0.32 -0.16 0.29 2.45 0.73 phosphoribosyl pyrophosphate synthetase 1 5631 PRPS1 -0.51 0.37 -0.53 3.56 3.25 0.14 -0.86 phosphoribosyl pyrophosphate synthetase 2 5634 PRPS2 -1.15 -1.99 -1.12 -1.72 -1.32 -1.00 -1.21 phosphoribosyl pyrophosphate synthetase 1-like 1 221823 PRPS1L1 -0.08 -1.08 -1.03 -0.55 -0.02 -0.35 -0.37 ribulose-5-phosphate-3-epimerase 6120 RPE -0.60 -0.30 -0.49 0.94 0.32 0.26 0.25 transaldolase 1 6888 TALDO1 -0.92 -0.24 0.04 -0.08 -0.77 0.24 -0.46 amidophosphoribosyltransferase 5471 PPAT 0.04 -0.34 -1.02 -1.88 -1.23 -0.74 -1.00 uridine monophosphate synthetase 7372 UMPS 2.58 0.55 -0.22 3.31 0.14 -0.65 0.10 Acetyl-Coa production solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1 6576 SLC25A1 -0.91 -0.73 -0.96 0.08 1.53 4.02 9.97 solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10 1468 SLC25A10 0.83 1.51 1.55 3.47 3.79 4.79 1.49 ATP citrate (pro-S)- 47 ACLY 2.37 -2.00 -1.01 -1.48 -0.97 -0.81 0.70 acyl-CoA synthetase short-chain family member 1 84532 ACSS1 2.10 0.46 0.57 -0.29 0.24 0.37 0.62 acyl-CoA synthetase short-chain family member 2 55902 ACSS2 2.12 0.31 6.36 -0.86 -0.02 -0.19 4.27 3-oxoacid CoA 1 5019 OXCT1 0.01 -0.64 -0.89 0.03 -0.01 -0.54 0.28 3-oxoacid CoA transferase 2 64064 OXCT2 0.19 -0.35 6.04 1.48 0.67 2.05 0.19 metabolism acetyl-CoA carboxylase alpha 31 ACACA -0.57 -1.43 -1.37 0.99 1.38 -0.90 -0.91 acetyl-CoA carboxylase beta 32 ACACB 1.05 -0.84 0.67 -0.43 -0.34 -0.89 1.01 fatty-acid synthase 2194 FASN 12.22 -1.04 -1.49 -0.37 -0.35 -0.68 -0.29 [acyl-carrier-] S-malonyltransferase 27349 MCAT 1.19 -0.39 0.65 0.15 -0.18 -0.44 0.25 oleoyl-[acyl-carrier-protein] 55301 OLAH 1.79 -0.63 0.04 -0.70 -0.38 -0.35 0.11 3-oxoacyl-[acyl-carrier-protein] synthase II 54995 OXSM 0.44 -0.07 -1.05 -0.35 -0.12 -0.19 -0.59 long chain fatty acyl elongase 79071 ELOVL6 0.87 0.23 -0.22 -0.15 -0.16 -0.88 0.03 stearoyl-CoA desaturase 6319 SCD 5.74 0.26 9.14 0.43 0.88 0.44 0.76 stearoyl-CoA desaturase 5 79966 SCD5 0.68 -0.30 -0.38 -0.22 -0.16 -0.39 0.25 glycerol-3-phosphate acyltransferase, mitochondrial 57678 GPAM 0.39 -0.55 -0.20 2.99 -0.20 -0.53 -0.79 1-acylglycerol-3-phosphate O-acyltransferase 1 10554 AGPAT1 -0.99 -0.21 0.29 0.40 -0.79 -0.50 1.18 1-acylglycerol-3-phosphate O-acyltransferase 2 10555 AGPAT2 0.89 0.31 -0.57 1.41 -1.16 -0.13 -0.32 1-acylglycerol-3-phosphate O-acyltransferase 6 137964 AGPAT6 -0.61 0.14 -0.19 1.25 -0.79 -0.64 -0.16 1-acylglycerol-3-phosphate O-acyltransferase 8 253558 LYCAT -0.58 -0.97 -0.89 -0.81 -0.29 -1.51 -0.48 1-acylglycerol-3-phosphate O-acyltransferase 3 56894 AGPAT3 -0.08 -0.39 -0.34 -1.05 0.42 -1.35 -0.17 1-acylglycerol-3-phosphate O-acyltransferase 4 56895 AGPAT4 -0.67 0.91 -0.46 -0.18 0.42 -0.90 -0.10 phosphatidic acid phosphatase type 2A 8611 PPAP2A -0.81 0.14 -0.40 0.32 1.10 0.77 0.49 phosphatidic acid phosphatase type 2C 8612 PPAP2C 0.02 0.57 0.06 -1.04 0.22 -0.51 0.80 phosphatidic acid phosphatase type 2B 8613 PPAP2B 0.38 1.07 0.28 -0.66 -0.37 -0.73 0.16 choline kinase alpha 1119 CHKA 2.46 0.15 -0.47 -1.28 -0.66 -0.18 -0.12 choline kinase beta 1120 CHKB -1.09 -0.42 -0.78 -1.99 -1.12 -0.63 -1.25 phosphate cytidylyltransferase 1, choline, alpha 5130 PCYT1A 0.71 -0.49 -0.58 0.16 -0.82 -0.95 0.04 phosphate cytidylyltransferase 1, choline, beta 9468 PCYT1B -0.66 0.10 -0.27 0.29 -0.91 -0.82 0.01 choline phosphotransferase 1 56994 CHPT1 0.22 -0.03 4.19 6.81 3.38 0.48 0.85 phospholipase D1, phosphatidylcholine-specific 5337 PLD1 -0.62 -0.57 -0.91 -0.17 -0.67 -0.95 -1.18 phospholipase D2 5338 PLD2 0.31 -0.26 -0.49 -0.14 -0.35 -0.62 -0.82 Cholesterol synthesis acetyl-Coenzyme A acetyltransferase 1 38 ACAT1 0.21 -1.00 -0.41 -0.55 -0.35 -1.67 -0.12 acetyl-Coenzyme A acetyltransferase 2 39 ACAT2 3.82 -0.22 -0.09 -0.15 1.15 0.24 -0.03 acetoacetyl-CoA synthetase 65985 AACS -0.16 0.22 0.18 -0.28 5.37 -0.39 -0.58 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) 3157 HMGCS1 20.76 -1.58 -1.18 3.49 2.62 0.48 2.37 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 3158 HMGCS2 0.44 4.74 3.77 2.85 0.21 -0.18 0.35 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 3156 HMGCR 0.85 2.95 0.13 -0.18 0.89 1.24 -0.17 mevalonate kinase 4598 MVK -1.00 1.56 0.08 -1.06 -0.72 1.01 0.74 phosphomevalonate kinase 10654 PMVK -0.34 2.33 -0.51 -0.09 0.00 1.45 1.49 diphosphomevalonate decarboxylase 4597 MVD 0.56 7.66 6.80 0.16 2.09 2.65 7.05 isopentenyl-diphosphate delta-isomerase 1 3422 IDI1 -0.31 -0.71 -0.18 0.04 -0.42 -0.77 -0.18 isopentenyl-diphosphate delta-isomerase 2 91734 IDI2 0.69 0.78 0.64 -0.34 -0.37 1.36 1.31 farnesyl diphosphate synthase 2224 FDPS 1.06 -0.57 3.14 -0.48 -0.46 0.07 0.46 geranylgeranyl diphosphate synthase 1 9453 GGPS1 -0.55 0.44 -0.95 1.97 2.05 0.41 1.33 farnesyl-diphosphate farnesyltransferase 2222 FDFT1 5.27 1.08 2.21 0.85 1.24 4.56 3.06 squalene monooxygenase 6713 SQLE 1.44 -0.92 3.49 -0.32 0.02 -0.56 3.34 lanosterol synthase 4047 LSS 4.12 1.14 -0.83 -0.52 -0.67 0.50 -0.29 lanosterol 14-alpha-demethylase 1595 CYP51A1 3.83 1.72 1.15 1.26 0.68 4.22 3.07 sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating) 50814 NSDHL 0.38 3.94 1.13 2.03 2.56 5.51 0.81 hydroxysteroid (17-beta) dehydrogenase 7 51478 HSD17B7 0.40 -0.47 -0.16 -0.26 -0.84 -0.97 -1.27 cholestenol delta-isomerase 10682 EBP 0.40 -0.71 1.72 0.50 0.26 -1.18 0.05 Lathosterol 5-desaturase 6309 SC5DL 0.20 -0.17 1.00 0.88 -0.17 0.06 -0.50 24-dehydrocholesterol reductase 1718 DHCR24 -1.11 0.80 0.37 0.48 3.17 2.12 0.60 7-dehydrocholesterol reductase 1717 DHCR7 -0.01 -1.03 -0.52 -0.72 -0.76 1.42 1.04 Hexosamine metabolism glutamine-fructose-6-phosphate transaminase 1 2673 GFPT1 -1.39 -0.10 -0.53 1.11 0.24 0.76 0.00 glutamine-fructose-6-phosphate transaminase 2 9945 GFPT2 -0.32 -0.20 1.49 -0.46 -0.52 0.20 -0.25 glucosamine-phosphate N-acetyltransferase 1 64841 GNPNAT1 0.26 -0.63 -0.17 0.32 -0.19 -0.73 2.67 phosphoglucomutase 3 5238 PGM3 -0.43 0.94 -0.44 0.05 1.25 0.67 1.37 UDP-N-acteylglucosamine pyrophosphorylase 1 6675 UAP1 -0.95 0.18 -1.16 -0.37 -0.32 0.21 -0.13 O-linked N-acetylglucosamine (GlcNAc) transferase 8473 OGT 0.54 -0.86 -0.33 0.31 -0.45 -0.32 -0.17 ROS metabolism superoxide dismutase 1, soluble 6647 SOD1 -0.61 -0.49 0.32 -0.21 0.87 -0.04 0.39 superoxide dismutase 2, mitochondrial 6648 SOD2 -0.75 1.34 0.88 0.67 -0.64 0.05 0.19 superoxide dismutase 3, extracellular 6649 SOD3 -0.09 -0.08 0.65 -0.65 0.39 0.70 -0.71 catalase 847 CAT -0.18 -0.14 -0.44 1.05 -0.78 -0.64 -0.26 glutathione peroxidase 6 257202 GPX6 0.69 2.22 0.32 0.37 0.62 1.23 0.53 glutathione peroxidase 1 2876 GPX1 -0.04 1.52 -0.33 0.94 1.02 -0.46 0.52 glutathione peroxidase 2 2877 GPX2 -1.05 0.44 1.17 0.64 2.32 0.20 0.19 glutathione peroxidase 3 2878 GPX3 0.08 0.15 -0.57 0.47 -1.06 0.62 0.11 glutathione peroxidase 4 2879 GPX4 -0.23 8.34 2.09 -0.51 2.11 2.73 1.41 glutathione peroxidase 5 2880 GPX5 0.18 0.04 -0.55 -0.05 -0.15 2.38 0.21 glutathione peroxidase 7 2882 GPX7 3.22 1.68 0.49 2.41 1.96 7.35 4.04 Oxidative mitochondrially encoded NADH dehydrogenase 1 4535 MT-ND1 -1.48 -1.82 -0.57 -0.79 -0.14 -1.47 -0.85 mitochondrially encoded NADH dehydrogenase 2 4536 MT-ND2 -0.80 5.71 4.90 -2.41 -1.35 0.27 2.08 mitochondrially encoded NADH dehydrogenase 3 4537 MT-ND3 0.11 6.61 0.15 -1.48 -1.06 -0.16 -1.04 mitochondrially encoded NADH dehydrogenase 4 4538 MT-ND4 -0.49 -0.46 0.91 1.91 1.31 0.02 0.52 mitochondrially encoded NADH dehydrogenase 5 4540 MT-ND5 -0.72 -1.12 1.79 -1.36 -0.78 -0.64 -0.75 mitochondrially encoded NADH dehydrogenase 6 4541 MT-ND6 -1.05 2.12 2.71 5.16 4.12 -0.35 -0.08 cytochrome oxidase deficient homolog 2 9997 SCO2 0.04 0.67 -0.59 -0.18 -0.45 3.44 1.17 ATPase inhibitory factor 1 93974 ATPIF1 -0.58 0.09 -0.07 -0.46 7.61 0.28 7.09 cytochrome c oxidase subunit IV isoform 1 1327 COX4I1 -0.47 -0.72 -0.58 -0.27 -0.62 -0.28 -0.75 cytochrome c oxidase subunit IV isoform 2 84701 COX4I2 -0.05 1.75 5.11 0.03 -0.31 0.44 0.01 cytochrome c oxidase subunit Va 9377 COX5A -0.46 0.01 -0.18 0.01 0.54 -0.57 -0.14 Metabolic regulation inducible factor 1, alpha subunit 3091 HIF1A -0.29 -1.69 0.57 -1.95 -1.18 -1.17 -0.54 Hypoxia-inducible factor 2 alpha 2034 EPAS1 -1.68 -1.40 -1.14 -2.31 -1.10 -1.06 -1.61 sterol regulatory element binding 1 6720 SREBF1 2.10 0.98 0.60 0.45 -0.59 4.39 12.31 sterol regulatory element binding transcription factor 2 6721 SREBF2 21.85 1.93 18.16 6.09 0.53 2.82 3.59 protein kinase, AMP-activated, alpha 1 catalytic subunit 5562 PRKAA1 0.11 -0.01 5.49 1.89 0.36 -0.52 -0.58 protein kinase, AMP-activated, alpha 2 catalytic subunit 5563 PRKAA2 -0.60 2.75 1.35 13.44 4.61 1.26 1.14 protein kinase, AMP-activated, beta 1 non-catalytic subunit 5564 PRKAB1 0.65 12.98 6.57 16.89 3.96 12.19 6.54 protein kinase, AMP-activated, beta 2 non-catalytic subunit 5565 PRKAB2 0.91 -0.11 -1.40 0.56 -0.10 0.48 -0.55 protein kinase, AMP-activated, gamma 1 non-catalytic subunit 5571 PRKAG1 -0.79 0.39 0.54 -0.96 -0.26 -0.68 -0.43 protein kinase, AMP-activated, gamma 2 non-catalytic subunit 51422 PRKAG2 -0.52 -2.33 -1.93 -2.00 -1.08 -0.84 -1.22 protein kinase, AMP-activated, gamma 3 non-catalytic subunit 53632 PRKAG3 19.27 12.35 6.74 1.07 -0.11 10.13 4.73 AKT1 kinase 207 AKT1 0.84 10.08 1.70 6.34 2.97 0.09 -0.40 AKT2 kinase 208 AKT2 1.76 -0.45 -0.89 4.96 3.41 0.63 2.38 AKT3 kinase 10000 AKT3 0.83 -1.13 -1.00 8.34 6.29 0.87 1.66 response element binding protein 51085 WBSCR14 -0.43 0.39 -0.17 1.27 2.72 4.99 2.02 Controls Controls - Plate 1 NA siCONTROL RISC-Free -0.11 -1.44 -0.54 -1.51 -0.79 -1.14 -0.61 Controls - Plate 2 NA siCONTROL RISC-Free -0.91 -1.53 -0.28 -2.11 -0.86 -1.33 -0.65 Controls - Plate 3 NA siCONTROL NT pool 2.88 1.08 1.15 3.88 0.66 0.40 1.31 Controls - Plate 4 NA siCONTROL NT pool -0.69 2.11 1.89 4.43 0.34 0.06 0.70 Controls - Plate 5 NA siCONTROL NT pool -1.03 1.32 1.61 3.64 0.37 -0.24 0.74 polo-like kinase 1 5347 PLK1 9.05 36.93 13.37 9.99 13.16 13.49 5.43 polo-like kinase 1 5347 PLK1 8.89 25.83 10.98 7.66 8.27 12.57 4.94 polo-like kinase 1 5347 PLK1 35.94 39.57 17.12 14.92 5.20 16.06 9.15 Controls - Plate 2 NA siCONTROL RISC-Free -0.67 -0.99 -0.38 -1.12 -0.71 -0.92 -0.71 Controls - Plate 3 NA siCONTROL RISC-Free -1.07 -1.11 -1.14 -1.36 -1.02 -1.70 -1.38 Controls - Plate 4 NA siCONTROL NT #1 0.03 0.55 -0.11 -0.77 1.23 -0.71 -0.67 Controls - Plate 5 NA siCONTROL NT #1 -0.27 1.08 1.13 -0.31 1.35 1.20 -0.46 Controls - Plate 6 NA siCONTROL NT #2 0.40 -0.14 -0.34 -0.80 0.15 -0.85 0.20 Controls - Plate 7 NA siCONTROL NT #2 0.27 0.04 0.58 -0.50 0.12 0.09 -0.10 Controls - Plate 8 NA siCONTROL NT #3 -0.79 -0.80 -0.60 0.27 0.52 -0.72 0.25 Controls - Plate 9 NA siCONTROL NT #3 -0.75 -0.56 -0.77 0.77 0.85 2.17 -0.72 Controls - Plate 10 NA siCONTROL NT #4 -0.80 -0.84 -0.21 -0.20 -0.62 -1.23 -0.63 Controls - Plate 11 NA siCONTROL NT #4 -1.10 -0.95 -0.38 -0.02 -0.67 -0.21 -0.72 Controls - Plate 12 NA siCONTROL NT pool 3.69 0.32 1.54 -0.67 0.49 0.88 0.29 Controls - Plate 13 NA siCONTROL NT pool 0.92 -0.23 -0.37 -0.27 0.18 -0.20 -0.45 Controls - Plate 14 NA siCONTROL NT pool -0.17 -0.43 -0.47 -0.62 0.21 -0.59 -0.03 polo-like kinase 1 5347 PLK1 17.09 11.50 14.46 13.74 10.56 6.41 7.98 polo-like kinase 1 5347 PLK1 13.23 11.39 12.24 14.31 9.70 6.48 7.51 polo-like kinase 1 5347 PLK1 14.56 11.05 7.05 13.06 6.82 8.39 4.61 Controls - Plate 3 NA siCONTROL RISC-Free -1.42 -1.24 -0.74 -1.18 -0.91 -1.38 -1.02 Controls - Plate 4 NA siCONTROL RISC-Free -0.64 -1.45 -0.80 -1.40 -1.09 -1.54 -1.09 Controls - Plate 5 NA siCONTROL NT #1 -0.92 0.14 0.15 -0.68 1.07 -0.93 -0.41 Controls - Plate 6 NA siCONTROL NT #1 -0.58 0.15 0.01 -0.14 1.28 -1.03 -0.67 Controls - Plate 7 NA siCONTROL NT #2 -0.39 -0.57 -0.06 -0.60 -0.51 -0.93 -0.27 Controls - Plate 8 NA siCONTROL NT #2 -0.57 0.27 0.39 -0.20 -0.21 0.32 -0.25 Controls - Plate 9 NA siCONTROL NT #3 -0.76 -0.98 0.03 0.16 0.31 -0.20 -0.10 Controls - Plate 10 NA siCONTROL NT #3 -0.54 -0.85 -0.21 0.81 0.84 -0.31 -0.44 Controls - Plate 11 NA siCONTROL NT #4 -0.87 -1.32 -0.15 -0.71 -0.75 -1.38 -1.12 Controls - Plate 12 NA siCONTROL NT #4 -1.00 -0.91 0.33 -0.10 -0.66 -0.59 -1.13 Controls - Plate 13 NA siCONTROL NT pool 1.65 -0.16 -0.02 -0.86 -0.02 -0.85 0.17 Controls - Plate 14 NA siCONTROL NT pool -0.31 0.18 -0.29 -0.75 0.06 -0.25 -0.75 Controls - Plate 15 NA siCONTROL NT pool -0.12 -0.34 -0.33 -0.96 0.09 -0.66 0.07 polo-like kinase 1 5347 PLK1 9.57 9.16 10.30 10.42 4.29 6.66 6.44 polo-like kinase 1 5347 PLK1 7.45 6.24 10.04 9.65 4.19 6.30 4.98 polo-like kinase 1 5347 PLK1 9.68 11.37 7.92 9.04 4.51 8.66 7.05 Effect of each siRNA SMARTpool on cell mass in each cell line/medium condition, expressed as fold of the average of the negative controls

RWPE1 DU145 DU145 LNCaP LNCaP PC3 PC3 Gene Name GeneID Gene Symbol (KGM) (FM) (LDM) (FM) (LDM) (FM) (LDM) Galactose metabolism galactokinase 1 2584 GALK1 0.82 0.82 0.67 0.96 1.12 0.80 0.82 galactokinase 2 2585 GALK2 0.73 0.90 0.81 0.88 1.02 0.79 0.89 galactose-1-phosphate uridylyltransferase 2592 GALT 0.84 0.61 0.83 0.80 0.90 1.04 1.07 phosphoglucomutase 1 5236 PGM1 0.77 0.91 1.04 1.28 1.32 1.21 1.41 glutaminase 2744 GLS 0.95 0.78 0.86 0.57 0.55 0.65 0.69 glutaminase 2 27165 GLS2 0.89 0.78 0.85 0.77 0.69 0.81 0.75 glutamate dehydrogenase 1 2746 GLUD1 0.80 0.93 0.95 0.93 1.03 1.31 1.40 glutamate dehydrogenase 2 2747 GLUD2 0.96 0.83 0.86 0.64 1.11 0.95 0.82 Glycolysis and glucose uptake solute carrier family 2 (facilitated glucose transporter), member 1 6513 SLC2A1 0.96 0.99 0.93 1.18 1.07 1.00 1.05 solute carrier family 2 (facilitated glucose transporter), member 4 6517 SLC2A4 0.83 0.90 0.99 0.77 0.63 0.88 0.81 hexokinase 1 3098 HK1 1.01 0.92 1.01 1.23 0.84 0.97 0.96 hexokinase 2 3099 HK2 0.91 0.84 0.78 0.98 0.52 0.82 0.86 hexokinase 3 3101 HK3 1.05 1.08 1.16 0.74 0.92 1.72 1.24 hexokinase 4 2645 HK4 0.97 0.93 1.14 0.90 1.40 0.98 0.97 glucose-6-phosphate isomerase 2821 GPI 0.82 0.83 0.76 0.77 0.87 0.87 0.63 phosphofructokinase, liver 5211 PFKL 1.10 1.03 0.81 0.76 0.68 1.01 1.15 6-phosphofructokinase, muscle 5213 PFKM 0.98 0.96 0.96 1.34 1.05 1.16 0.85 6-phosphofructokinase, platelet 5214 PFKP 0.71 0.66 0.74 0.73 1.05 0.77 0.87 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 5207 PFKFB1 0.68 0.62 0.66 0.73 0.72 0.59 0.76 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 5208 PFKFB2 0.97 0.88 0.96 0.77 0.94 0.80 0.89 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 5209 PFKFB3 0.74 0.61 0.67 0.83 0.58 0.91 1.01 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 5210 PFKFB4 0.79 0.67 0.65 0.79 0.80 0.66 0.84 TP53-induced glycolysis and apoptosis regulator 57103 C12orf5 1.10 1.12 1.14 0.95 1.01 0.98 0.89 aldolase A 226 ALDOA 0.99 0.78 0.94 0.78 1.09 0.55 0.78 aldolase B 229 ALDOB 0.78 0.77 0.74 0.82 1.33 0.85 1.04 aldolase C 230 ALDOC 0.88 0.80 0.81 0.54 0.61 0.99 0.62 triose phosphate isomerase 7167 TPI1 1.00 1.00 1.12 0.63 1.10 1.44 1.49 glyceraldehyde-3-phosphate dehydrogenase 2597 GAPDH 1.01 1.08 1.17 1.08 0.84 1.22 1.15 glyceraldehyde-3-phosphate dehydrogenase, spermatogenic 26330 GAPDHS 0.89 0.93 1.01 0.91 1.15 0.83 0.83 phosphoglycerate kinase 1 5230 PGK1 1.05 1.11 0.73 0.80 0.60 1.06 1.17 phosphoglycerate kinase 2 5232 PGK2 1.05 1.09 1.02 0.63 0.85 1.00 0.88 phosphoglycerate mutase family member 4 441531 PGAM4 1.05 1.01 1.14 0.94 1.29 0.98 0.71 phosphoglycerate mutase 1 5223 PGAM1 0.85 1.29 1.03 0.81 0.71 1.04 1.01 phosphoglycerate mutase 2 5224 PGAM2 1.01 1.03 0.98 1.15 1.08 0.94 1.15 2,3-bisphosphoglycerate mutase 669 BPGM 0.82 0.92 0.77 1.06 1.23 0.90 1.15 enolase 1 2023 ENO1 1.16 1.04 1.04 1.42 1.02 1.18 1.10 enolase 2 2026 ENO2 0.84 0.81 0.84 1.00 0.98 0.95 1.06 enolase 3 2027 ENO3 0.88 0.69 0.83 0.91 1.02 0.80 1.14 pyruvate kinase, liver and RBC 5313 PKLR 0.87 0.95 0.48 0.97 1.13 1.05 1.09 pyruvate kinase, muscle 5315 PKM2 0.79 0.87 0.87 0.97 1.27 0.67 0.63 lactate dehydrogenase A 3939 LDHA 0.82 0.88 1.04 1.02 1.07 0.94 1.01 lactate dehydrogenase B 3945 LDHB 0.99 0.94 1.17 1.23 0.90 0.93 0.88 lactate dehydrogenase C 3948 LDHC 0.97 0.72 1.01 0.68 0.77 0.76 0.74 lactate dehydrogenase A-like 6A 160287 LDHAL6A 0.83 0.97 0.91 1.10 1.17 0.88 0.88 lactate dehydrogenase A-like 6B 92483 LDHAL6B 0.91 0.73 0.85 0.38 0.70 0.71 0.88 solute carrier family 16, member 1 (monocarboxylic acid transporter 1) 6566 SLC16A1 0.96 0.99 0.93 0.62 1.23 0.94 0.88 solute carrier family 16, member 7 (monocarboxylic acid transporter 2) 9194 SLC16A7 0.96 0.94 0.93 1.12 1.11 0.91 1.03 solute carrier family 16, member 3 (monocarboxylic acid transporter 4) 9123 SLC16A3 0.63 0.34 0.40 0.56 0.58 0.46 0.69 solute carrier family 16, member 8 (monocarboxylic acid transporter 3) 23539 SLC16A8 0.66 0.68 0.84 0.82 0.81 0.48 0.67 solute carrier family 16, member 4 (monocarboxylic acid transporter 5 9122 SLC16A4 1.02 0.89 1.12 0.76 0.91 0.77 0.84 TCA cycle pyruvate dehydrogenase (lipoamide) alpha 1 5160 PDHA1 0.96 0.93 0.89 1.09 1.21 0.89 0.93 pyruvate dehydrogenase (lipoamide) alpha 2 5161 PDHA2 0.86 0.47 0.58 0.69 0.77 0.79 0.86 pyruvate dehydrogenase (lipoamide) beta t 5162 PDHB 0.88 0.85 1.03 0.96 0.72 0.78 0.95 dihydrolipoamide S-acetyltransferase 1737 DLAT 0.97 0.91 0.83 0.86 0.75 0.82 0.90 dihydrolipoamide dehydrogenase 1738 DLD 0.93 0.97 1.01 0.78 0.92 1.03 1.20 pyruvate dehydrogenase kinase, isozyme 1 5163 PDK1 0.96 0.84 0.87 0.97 0.99 0.97 0.97 pyruvate dehydrogenase kinase, isozyme 2 5164 PDK2 0.90 1.02 0.73 1.06 0.86 0.89 1.06 pyruvate dehydrogenase kinase, isozyme 3 5165 PDK3 0.74 0.85 0.71 0.57 0.74 1.02 0.73 pyruvate dehydrogenase kinase, isozyme 4 5166 PDK4 0.97 0.78 0.73 0.48 0.68 0.73 0.69 pyruvate dehyrogenase phosphatase catalytic subunit 1 54704 PPM2C 0.82 0.64 0.82 0.80 0.94 1.27 0.93 pyruvate dehyrogenase phosphatase catalytic subunit 2 57546 PDP2 0.87 1.18 0.85 0.94 0.82 0.79 0.66 citrate synthase 1431 CS 0.88 0.88 0.93 0.65 0.67 0.86 0.96 aconitate hydratase 1, soluble 48 ACO1 0.78 0.94 0.71 0.73 0.57 0.57 0.77 aconitase 2, mitochondrial 50 ACO2 0.70 0.74 0.62 0.83 0.81 0.68 0.88 similar to aconitase 2 441996 LOC441996 0.69 0.43 0.58 0.72 0.53 0.31 0.54 isocitrate dehydrogenase 1 (NADP+), soluble 3417 IDH1 1.21 1.12 1.10 0.99 1.29 0.91 0.90 isocitrate dehydrogenase 2 (NADP+), mitochondrial 3418 IDH2 1.05 1.11 0.85 1.26 1.18 1.08 0.96 isocitrate dehydrogenase 3 (NAD+) alpha 3419 IDH3A 0.91 0.97 1.06 1.16 0.92 0.71 0.96 isocitrate dehydrogenase 3 (NAD+) beta 3420 IDH3B 0.84 0.71 0.68 0.69 0.76 0.50 0.64 isocitrate dehydrogenase 3 (NAD+) gamma 3421 IDH3G 0.90 0.74 0.82 0.64 0.76 0.81 0.86 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 4967 OGDH 0.74 0.65 0.85 0.91 0.74 0.88 0.70 dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) 1743 DLST 0.73 0.92 0.64 0.95 0.94 1.33 1.09 succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial pseudogene 283398 LOC283398 1.03 1.10 1.06 0.75 0.95 0.95 0.95 succinate-CoA ligase, GDP-forming, beta subunit 8801 SUCLG2 1.07 0.99 1.08 0.92 0.76 0.67 0.67 succinate-CoA ligase, alpha subunit 8802 SUCLG1 0.87 0.95 0.93 0.81 0.72 0.70 0.79 uccinate-CoA ligase, ADP-forming, beta subunit e 8803 SUCLA2 0.77 0.87 0.72 1.07 1.11 0.74 0.82 succinate dehydrogenase complex, subunit A 6389 SDHA 0.95 1.06 0.89 1.26 1.09 0.93 0.86 succinate dehydrogenase complex, subunit B 6390 SDHB 0.92 0.98 1.15 0.72 0.83 0.71 0.59 succinate dehydrogenase complex, subunit C 6391 SDHC 0.98 1.20 1.43 1.16 0.70 1.14 1.04 succinate dehydrogenase complex, subunit D 6392 SDHD 0.58 0.89 0.74 0.57 1.04 1.02 0.86 fumarate hydratase 2271 FH 0.95 1.03 1.08 0.89 0.99 1.04 0.95 malate dehydrogenase 2, NAD (mitochondrial) 4191 MDH2 0.95 1.06 0.94 1.53 0.72 1.04 0.89 Anapleurosis and mitochondrial shuttles malate dehydrogenase 1, NAD (soluble) 4190 MDH1 0.86 0.94 0.91 0.63 1.10 0.79 0.66 aspartate aminotransferase 1 2805 GOT1 0.93 0.86 0.85 0.80 1.04 0.62 0.80 aspartate aminotransferase 2 2806 GOT2 1.00 0.91 1.12 0.94 0.79 0.87 0.88 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 8402 SLC25A11 0.86 0.68 0.80 0.67 0.67 0.49 0.58 glycerol-3-phosphate dehydrogenase 1 (soluble) 2819 GPD1 0.85 0.90 0.69 0.78 1.15 0.98 1.05 glycerol-3-phosphate dehydrogenase 1-like 23171 GPD1L 0.91 0.85 0.88 0.91 1.10 0.69 0.86 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) 2820 GPD2 1.05 1.11 0.95 1.11 1.18 1.12 1.11 pyruvate carboxylase 5091 PC 0.84 0.75 0.82 1.23 0.93 0.98 0.96 malic enzyme 1, NADP(+)-dependent, cytosolic 4199 ME1 0.66 0.68 0.56 0.66 0.59 0.69 0.63 malic enzyme 3, NADP(+)-dependent, cytosolic 10873 ME3 1.13 0.96 1.02 0.78 0.81 0.76 0.82 malic enzyme 2, NAD(+)-dependent, mitochondrial 4200 ME2 0.59 0.45 0.38 0.68 0.92 0.52 0.52 Aminoacid uptake and metabolism glutamine synthetase 2752 GLUL 0.93 1.03 0.88 0.89 0.74 0.99 0.87 alanine aminotransferase 2875 GPT 0.94 0.81 0.90 0.70 0.62 0.84 0.81 alanine transaminase 2 84706 GPT2 0.79 0.99 0.59 0.90 0.80 0.82 0.78 D-aspartate oxidase 8528 DDO 0.90 1.06 1.07 0.99 0.85 1.11 0.95 ilvB (bacterial acetolactate synthase)-like 10994 ILVBL 0.80 0.84 0.78 0.59 1.03 0.99 0.97 alanine-glyoxylate aminotransferase 189 AGXT 0.69 0.70 0.77 0.86 0.83 0.84 0.93 alanine-glyoxylate aminotransferase 2 64902 AGXT2 0.71 0.71 0.60 0.81 0.78 0.48 0.54 phosphoglycerate dehydrogenase 26227 PHGDH 1.01 0.80 0.84 1.04 0.55 0.64 0.68 phosphoserine aminotransferase 29968 PSAT1 0.96 1.02 1.04 1.39 1.02 0.77 0.96 ASCT2, solute carrier family 1 (neutral amino acid transporter), member 5 6510 SLC1A5 1.12 0.99 0.89 1.02 1.16 0.94 0.90 LAT1, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 8140 SLC7A5 0.51 0.80 0.91 1.31 0.85 0.88 0.97 carbamoyl-phosphate synthase 790 CAD 0.57 0.90 1.07 0.89 0.80 0.67 1.25 ornithine decarboxylase 4953 ODC1 0.81 0.83 0.76 0.57 0.98 0.71 1.03 serine hydroxymethyltransferase 1 (soluble) 6470 SHMT1 0.85 0.97 0.93 0.69 0.69 0.90 0.94 serine hydroxymethyltransferase 2 (mitochondrial 6472 SHMT2 0.77 0.91 0.94 0.52 0.81 0.99 1.16 Pentose phosphate pathway glucose-6-phosphate dehydrogenase 2539 G6PD 0.76 0.83 0.63 0.44 0.61 0.90 0.93 transketolase 7086 TKT 0.91 0.81 1.12 0.81 0.98 1.28 0.99 transketolase-like 1 8277 TKTL1 0.79 0.71 0.85 0.65 0.91 0.83 0.59 transketolase-like 2 84076 TKTL2 0.79 0.92 0.96 0.80 0.75 0.69 0.61 6-phosphogluconolactonase 25796 PGLS 0.77 0.73 0.76 0.90 0.75 0.92 0.99 hexose-6-phosphate dehydrogenase 9563 H6PD 0.86 0.82 0.83 0.83 0.55 0.73 0.69 6-phosphogluconate dehydrogenase 5226 PGD 0.63 0.61 0.72 0.94 0.65 0.90 0.87 ribose 5-phosphate isomerase 22934 RPIA 0.67 0.78 0.64 0.94 0.78 0.57 0.58 phosphoribosyl pyrophosphate synthetase 1 5631 PRPS1 0.96 0.88 0.95 0.76 0.52 0.91 0.95 phosphoribosyl pyrophosphate synthetase 2 5634 PRPS2 1.21 1.20 1.15 1.59 1.19 0.94 1.03 phosphoribosyl pyrophosphate synthetase 1-like 1 221823 PRPS1L1 0.85 1.24 1.50 1.14 0.97 1.04 0.78 ribulose-5-phosphate-3-epimerase 6120 RPE 1.01 0.89 0.98 0.75 0.91 0.85 0.86 transaldolase 1 6888 TALDO1 0.93 0.89 1.08 0.90 1.07 0.84 0.95 amidophosphoribosyltransferase 5471 PPAT 1.03 0.70 0.94 1.39 0.99 0.93 0.86 uridine monophosphate synthetase 7372 UMPS 0.71 0.98 1.19 0.81 0.65 1.12 1.31 Acetyl-Coa production solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1 6576 SLC25A1 1.00 0.98 1.08 1.29 1.19 0.94 0.74 solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10 1468 SLC25A10 0.71 0.64 0.57 0.44 0.31 0.52 0.67 ATP citrate (pro-S)-lyase 47 ACLY 0.85 1.09 1.14 1.39 1.07 0.98 0.99 acyl-CoA synthetase short-chain family member 1 84532 ACSS1 0.77 0.77 0.75 0.93 0.73 0.77 0.64 acyl-CoA synthetase short-chain family member 2 55902 ACSS2 0.73 0.89 0.66 1.32 1.12 1.33 0.94 3-oxoacid CoA transferase 1 5019 OXCT1 0.97 0.84 0.85 0.87 0.83 0.90 0.89 3-oxoacid CoA transferase 2 64064 OXCT2 0.70 0.88 0.53 0.59 0.66 0.56 0.71 Fatty acid metabolism acetyl-CoA carboxylase alpha 31 ACACA 0.77 0.87 1.01 1.21 1.17 1.08 1.07 acetyl-CoA carboxylase beta 32 ACACB 0.86 1.05 0.87 1.04 0.89 1.11 0.97 fatty-acid synthase 2194 FASN 0.36 0.84 0.87 0.70 1.11 0.90 0.81 [acyl-carrier-protein] S-malonyltransferase 27349 MCAT 0.87 0.97 0.94 0.89 0.95 0.93 0.84 oleoyl-[acyl-carrier-protein] hydrolase 55301 OLAH 0.91 0.99 1.03 1.13 0.97 1.00 0.82 3-oxoacyl-[acyl-carrier-protein] synthase II 54995 OXSM 1.04 0.88 1.20 0.94 1.00 1.03 0.92 long chain fatty acyl elongase 79071 ELOVL6 0.92 0.74 0.76 0.91 0.95 1.17 1.00 stearoyl-CoA desaturase 6319 SCD 0.62 0.87 0.39 0.80 0.90 0.88 0.81 stearoyl-CoA desaturase 5 79966 SCD5 0.88 0.93 0.92 0.90 0.75 0.90 0.94 glycerol-3-phosphate acyltransferase, mitochondrial 57678 GPAM 0.79 0.94 0.95 0.65 0.87 0.96 0.83 1-acylglycerol-3-phosphate O-acyltransferase 1 10554 AGPAT1 0.92 0.91 0.86 1.01 1.06 1.05 0.60 1-acylglycerol-3-phosphate O-acyltransferase 2 10555 AGPAT2 0.69 0.84 1.01 0.67 1.04 0.94 0.94 1-acylglycerol-3-phosphate O-acyltransferase 6 137964 AGPAT6 0.98 0.77 0.76 0.69 1.01 0.89 0.88 1-acylglycerol-3-phosphate O-acyltransferase 8 253558 LYCAT 1.02 1.04 1.18 1.13 1.18 1.23 0.98 1-acylglycerol-3-phosphate O-acyltransferase 3 56894 AGPAT3 0.92 1.02 1.00 1.33 1.18 1.33 1.12 1-acylglycerol-3-phosphate O-acyltransferase 4 56895 AGPAT4 0.98 0.75 0.91 0.88 0.82 0.98 0.84 phosphatidic acid phosphatase type 2A 8611 PPAP2A 0.98 0.78 0.91 0.82 0.70 0.71 0.58 phosphatidic acid phosphatase type 2C 8612 PPAP2C 0.69 0.67 0.70 1.26 0.90 0.91 0.58 phosphatidic acid phosphatase type 2B 8613 PPAP2B 0.84 0.66 0.65 1.12 1.12 0.94 0.78 choline kinase alpha 1119 CHKA 0.66 1.03 1.12 0.80 0.74 0.86 0.96 choline kinase beta 1120 CHKB 1.02 1.05 1.16 1.24 1.08 1.09 1.13 phosphate cytidylyltransferase 1, choline, alpha 5130 PCYT1A 0.83 0.91 0.97 0.85 1.04 1.08 0.92 phosphate cytidylyltransferase 1, choline, beta 9468 PCYT1B 0.88 0.85 0.86 0.86 1.22 1.11 0.84 choline phosphotransferase 1 56994 CHPT1 0.78 0.97 0.65 0.48 1.16 0.85 0.91 phospholipase D1, phosphatidylcholine-specific 5337 PLD1 0.96 0.96 1.08 0.88 0.92 0.91 0.99 phospholipase D2 5338 PLD2 0.92 0.91 0.87 0.96 1.10 1.03 1.05 Cholesterol synthesis acetyl-Coenzyme A acetyltransferase 1 38 ACAT1 0.94 1.05 0.99 1.00 0.87 1.28 0.96 acetyl-Coenzyme A acetyltransferase 2 39 ACAT2 0.77 0.85 0.71 0.88 0.65 0.80 0.73 acetoacetyl-CoA synthetase 65985 AACS 0.81 0.83 0.88 1.04 0.75 0.91 0.97 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) 3157 HMGCS1 0.61 0.98 1.06 0.94 0.75 0.93 0.85 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 3158 HMGCS2 0.94 0.76 0.77 0.74 1.14 0.96 1.05 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 3156 HMGCR 0.94 0.86 1.01 0.93 1.36 0.77 0.77 mevalonate kinase 4598 MVK 0.95 0.86 0.87 0.98 0.58 0.87 0.91 phosphomevalonate kinase 10654 PMVK 1.13 0.86 0.93 1.37 0.79 0.77 0.71 diphosphomevalonate decarboxylase 4597 MVD 1.02 0.69 0.55 1.00 0.73 0.72 0.55 isopentenyl-diphosphate delta-isomerase 1 3422 IDI1 0.96 1.00 0.99 0.81 0.76 1.06 0.96 isopentenyl-diphosphate delta-isomerase 2 91734 IDI2 0.83 0.74 0.82 0.92 0.79 0.79 0.78 farnesyl diphosphate synthase 2224 FDPS 0.85 1.05 0.81 1.02 0.76 1.06 0.87 geranylgeranyl diphosphate synthase 1 9453 GGPS1 1.08 0.94 0.88 0.72 0.64 0.74 0.72 farnesyl-diphosphate farnesyltransferase 2222 FDFT1 0.54 0.64 0.47 0.64 0.49 0.43 0.43 squalene monooxygenase 6713 SQLE 0.83 1.17 0.78 0.93 0.93 1.08 1.07 lanosterol synthase 4047 LSS 0.95 0.92 1.10 1.07 0.94 0.91 0.84 lanosterol 14-alpha-demethylase 1595 CYP51A1 0.72 0.63 0.65 0.65 0.73 0.49 0.64 sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating) 50814 NSDHL 0.86 0.52 0.71 0.60 0.63 0.62 0.74 hydroxysteroid (17-beta) dehydrogenase 7 51478 HSD17B7 0.90 0.94 0.98 0.92 1.10 0.96 1.12 cholestenol delta-isomerase 10682 EBP 0.87 1.05 0.90 0.74 0.79 1.40 1.21 Lathosterol 5-desaturase 6309 SC5DL 0.79 0.82 0.72 0.64 0.69 0.83 0.94 24-dehydrocholesterol reductase 1718 DHCR24 0.85 0.74 0.72 0.76 0.62 0.66 0.58 7-dehydrocholesterol reductase 1717 DHCR7 0.94 0.89 0.94 0.96 0.96 0.84 0.78 Hexosamine metabolism glutamine-fructose-6-phosphate transaminase 1 2673 GFPT1 1.06 0.84 1.00 0.69 0.86 0.74 0.80 glutamine-fructose-6-phosphate transaminase 2 9945 GFPT2 0.86 0.93 0.82 1.02 1.21 0.85 0.87 glucosamine-phosphate N-acetyltransferase 1 64841 GNPNAT1 0.86 0.97 0.94 0.81 1.01 1.19 0.79 phosphoglucomutase 3 5238 PGM3 0.89 0.67 0.82 0.98 0.88 1.04 0.87 UDP-N-acteylglucosamine pyrophosphorylase 1 6675 UAP1 0.85 0.69 0.90 0.92 0.74 0.66 0.73 O-linked N-acetylglucosamine (GlcNAc) transferase 8473 OGT 0.82 1.01 1.06 0.76 0.87 0.73 0.88 ROS metabolism superoxide dismutase 1, soluble 6647 SOD1 0.88 0.97 0.95 0.89 0.98 1.13 1.18 superoxide dismutase 2, mitochondrial 6648 SOD2 0.92 0.73 0.86 0.72 0.85 0.92 0.93 superoxide dismutase 3, extracellular 6649 SOD3 0.89 0.85 0.88 1.02 0.90 0.79 1.11 catalase 847 CAT 0.96 0.82 0.93 0.63 0.94 0.95 0.77 glutathione peroxidase 6 257202 GPX6 0.78 0.62 0.74 0.75 0.72 0.61 0.60 glutathione peroxidase 1 2876 GPX1 0.80 0.53 0.64 0.68 0.84 0.81 0.76 glutathione peroxidase 2 2877 GPX2 0.95 0.71 0.66 0.70 0.75 0.94 0.79 glutathione peroxidase 3 2878 GPX3 0.88 0.69 0.76 0.69 1.01 0.59 0.72 glutathione peroxidase 4 2879 GPX4 0.71 0.30 0.64 1.09 0.74 0.70 0.98 glutathione peroxidase 5 2880 GPX5 0.68 0.74 0.61 0.88 0.77 0.51 0.62 glutathione peroxidase 7 2882 GPX7 0.52 0.62 0.64 0.48 0.52 0.41 0.54 Oxidative phosphorylation mitochondrially encoded NADH dehydrogenase 1 4535 MT-ND1 1.23 0.95 1.09 1.31 1.20 1.10 1.15 mitochondrially encoded NADH dehydrogenase 2 4536 MT-ND2 1.22 0.79 0.72 2.38 0.63 1.24 1.36 mitochondrially encoded NADH dehydrogenase 3 4537 MT-ND3 0.90 0.64 0.87 1.26 0.88 0.86 0.84 mitochondrially encoded NADH dehydrogenase 4 4538 MT-ND4 1.08 0.91 0.88 0.96 0.98 1.00 0.86 mitochondrially encoded NADH dehydrogenase 5 4540 MT-ND5 1.05 1.02 0.90 1.34 1.23 0.99 1.01 mitochondrially encoded NADH dehydrogenase 6 4541 MT-ND6 0.97 0.79 0.81 0.83 0.46 1.04 1.14 cytochrome oxidase deficient homolog 2 9997 SCO2 0.73 0.75 0.82 0.91 0.82 0.56 0.62 ATPase inhibitory factor 1 93974 ATPIF1 0.89 0.78 0.91 0.97 0.46 0.82 0.65 cytochrome c oxidase subunit IV isoform 1 1327 COX4I1 0.93 1.06 1.12 0.93 1.00 0.83 1.07 cytochrome c oxidase subunit IV isoform 2 84701 COX4I2 0.77 0.63 0.49 0.84 0.89 0.81 0.88 cytochrome c oxidase subunit Va 9377 COX5A 0.85 0.76 0.65 0.88 0.63 0.87 0.62 Metabolic regulation hypoxia inducible factor 1, alpha subunit 3091 HIF1A 1.05 1.00 0.96 0.87 0.99 1.02 0.91 Hypoxia-inducible factor 2 alpha 2034 EPAS1 1.34 0.97 1.15 1.74 1.10 1.01 0.97 sterol regulatory element binding transcription factor 1 6720 SREBF1 0.80 0.66 0.83 0.91 0.99 0.83 0.75 sterol regulatory element binding transcription factor 2 6721 SREBF2 0.54 0.88 0.40 0.67 0.78 0.72 0.60 protein kinase, AMP-activated, alpha 1 catalytic subunit 5562 PRKAA1 0.83 1.15 0.73 0.67 0.99 1.22 1.21 protein kinase, AMP-activated, alpha 2 catalytic subunit 5563 PRKAA2 0.87 0.84 0.90 0.40 0.80 1.00 1.00 protein kinase, AMP-activated, beta 1 non-catalytic subunit 5564 PRKAB1 0.94 0.56 0.51 0.60 0.76 0.65 0.62 protein kinase, AMP-activated, beta 2 non-catalytic subunit 5565 PRKAB2 0.72 0.72 0.93 0.71 1.17 0.84 0.94 protein kinase, AMP-activated, gamma 1 non-catalytic subunit 5571 PRKAG1 1.02 1.08 1.07 1.11 1.05 1.11 1.17 protein kinase, AMP-activated, gamma 2 non-catalytic subunit 51422 PRKAG2 1.14 1.00 1.30 1.55 0.73 1.09 1.15 protein kinase, AMP-activated, gamma 3 non-catalytic subunit 53632 PRKAG3 0.52 0.73 0.56 0.58 1.11 0.70 0.67 AKT1 kinase 207 AKT1 0.84 0.62 0.70 0.84 0.94 0.91 0.98 AKT2 kinase 208 AKT2 0.82 0.87 0.90 0.82 1.28 0.89 0.78 AKT3 kinase 10000 AKT3 0.90 1.01 1.27 0.55 0.60 1.01 0.93 carbohydrate response element binding protein 51085 WBSCR14 0.92 0.73 0.80 0.62 0.52 0.51 0.41 Controls Controls - Plate 1 NA siCONTROL RISC-Free 0.97 1.03 1.08 1.13 1.57 1.09 1.13 Controls - Plate 2 NA siCONTROL RISC-Free 1.22 1.07 1.12 1.69 0.65 1.20 1.32 Controls - Plate 3 NA siCONTROL NT pool 0.86 0.92 0.89 0.86 1.16 0.84 0.95 Controls - Plate 4 NA siCONTROL NT pool 0.93 0.87 0.77 0.75 0.85 0.89 0.99 Controls - Plate 5 NA siCONTROL NT pool 1.02 0.98 0.76 0.86 0.96 0.96 1.01 polo-like kinase 1 5347 PLK1 0.49 0.16 0.18 0.71 0.97 0.43 0.53 polo-like kinase 1 5347 PLK1 0.45 0.20 0.20 0.74 0.80 0.46 0.53 polo-like kinase 1 5347 PLK1 0.25 0.12 0.18 0.44 0.80 0.38 0.42 Controls - Plate 2 NA siCONTROL RISC-Free 1.06 1.12 1.08 1.34 1.12 1.08 1.25 Controls - Plate 3 NA siCONTROL RISC-Free 1.05 1.11 1.15 1.47 1.26 1.32 1.28 Controls - Plate 4 NA siCONTROL NT #1 1.00 0.88 0.99 1.29 1.04 1.19 1.20 Controls - Plate 5 NA siCONTROL NT #1 0.99 0.83 0.95 1.06 1.08 0.83 1.33 Controls - Plate 6 NA siCONTROL NT #2 0.92 0.97 0.93 1.24 0.98 1.06 0.92 Controls - Plate 7 NA siCONTROL NT #2 0.84 0.91 0.89 1.08 0.96 0.81 0.94 Controls - Plate 8 NA siCONTROL NT #3 1.17 1.08 1.26 0.87 0.88 1.16 0.80 Controls - Plate 9 NA siCONTROL NT #3 1.13 0.97 1.27 0.71 0.83 0.60 0.82 Controls - Plate 10 NA siCONTROL NT #4 1.09 1.07 1.07 0.92 0.79 1.11 0.97 Controls - Plate 11 NA siCONTROL NT #4 1.03 1.15 1.14 0.84 0.75 0.93 0.95 Controls - Plate 12 NA siCONTROL NT pool 0.85 0.99 0.96 1.29 1.16 0.97 1.18 Controls - Plate 13 NA siCONTROL NT pool 0.91 1.03 0.96 1.05 1.08 1.04 1.12 Controls - Plate 14 NA siCONTROL NT pool 0.97 1.01 1.02 1.24 1.14 1.04 1.01 polo-like kinase 1 5347 PLK1 0.44 0.23 0.19 0.39 0.54 0.59 0.41 polo-like kinase 1 5347 PLK1 0.33 0.24 0.20 0.37 0.50 0.52 0.40 polo-like kinase 1 5347 PLK1 0.32 0.22 0.20 0.40 0.57 0.43 0.32 Controls - Plate 3 NA siCONTROL RISC-Free 1.04 1.15 1.09 1.36 1.11 1.33 1.31 Controls - Plate 4 NA siCONTROL RISC-Free 1.05 1.22 1.07 1.48 1.13 1.47 1.31 Controls - Plate 5 NA siCONTROL NT #1 1.10 0.94 1.04 1.28 1.04 1.37 1.17 Controls - Plate 6 NA siCONTROL NT #1 0.97 0.99 1.01 1.09 1.09 1.40 1.35 Controls - Plate 7 NA siCONTROL NT #2 0.99 1.00 0.99 1.12 0.96 1.18 1.01 Controls - Plate 8 NA siCONTROL NT #2 0.81 0.87 0.90 1.00 0.92 0.86 1.02 Controls - Plate 9 NA siCONTROL NT #3 1.10 1.05 1.24 0.84 0.84 1.10 0.89 Controls - Plate 10 NA siCONTROL NT #3 0.98 1.01 1.23 0.73 0.81 0.98 0.93 Controls - Plate 11 NA siCONTROL NT #4 1.09 1.21 1.20 1.06 0.72 1.35 1.05 Controls - Plate 12 NA siCONTROL NT #4 0.97 1.06 1.07 0.89 0.67 1.02 1.04 Controls - Plate 13 NA siCONTROL NT pool 0.90 1.06 1.06 1.34 1.09 1.29 1.13 Controls - Plate 14 NA siCONTROL NT pool 0.99 0.96 1.03 1.26 0.97 1.09 1.25 Controls - Plate 15 NA siCONTROL NT pool 1.01 0.98 0.95 1.34 1.04 1.17 0.98 polo-like kinase 1 5347 PLK1 0.42 0.27 0.23 0.46 0.60 0.65 0.39 polo-like kinase 1 5347 PLK1 0.37 0.34 0.21 0.47 0.54 0.56 0.41 polo-like kinase 1 5347 PLK1 0.38 0.21 0.19 0.47 0.55 0.45 0.37