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2006.01) C12P 7/42 (2006.01) TR), OAPI (BF, BJ, CF, CG, Cl, CM, GA, GN, GQ, GW, C12N 9/02 (2006.01) KM, ML, MR, NE, SN, TD, TG

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2006.01) C12P 7/42 (2006.01) TR), OAPI (BF, BJ, CF, CG, Cl, CM, GA, GN, GQ, GW, C12N 9/02 (2006.01) KM, ML, MR, NE, SN, TD, TG ( (51) International Patent Classification: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, C12N 15/52 (2006.01) C12P 7/42 (2006.01) TR), OAPI (BF, BJ, CF, CG, Cl, CM, GA, GN, GQ, GW, C12N 9/02 (2006.01) KM, ML, MR, NE, SN, TD, TG). (21) International Application Number: Published: PCT/BR2020/050052 — with international search report (Art. 21(3)) (22) International Filing Date: — with sequence listing part of description (Rule 5.2(a)) 20 February 2020 (20.02.2020) — in black and white; the international application as filed contained color or greyscale and is available for download (25) Filing Language: English from PATENTSCOPE (26) Publication Language: English (30) Priority Data: 62/808,247 20 February 2019 (20.02.2019) US (71) Applicant: BRASKEM S.A. [BR/BR]; Rua Eteno, 1.561, Polo Petroquimico, 42810-000 Camagari/BA (BR). (72) Inventors: KOCH, Daniel Johannes; c/o Braskem S.A., Rua Eteno, 1.561, Polo Petroquimico, 42810-000 Ca- magari/BA (BR). PEDERSON PARIZZI, Lucas; c/o Braskem S.A., Rua Eteno, 1.561, Polo Petroquimico, 42810-000 Camagari/BA (BR). GALZERANI, Felipe; c/ o Braskem S.A., Rua Eteno, 1.561, Polo Petroquimico, 42810-000 Camagari/BA (BR). (74) Agent: DANNEMANN, SIEMSEN, BIGLER & IPANE- MA MOREIRA; Rua Marques de Olinda, 70 - Botafogo, 2225 1-040 Rio de Janeiro - RJ (BR). (81) Designated States (unless otherwise indicated, for every kind of national protection available) : AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, WS, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available) : ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (54) Title: MICROORGANISMS AND METHODS FOR THE PRODUCTION OF OXYGENATED COMPOUNDS FROM HEX- OSES (57) Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product. MICROORGANISMS AND METHODS FOR THE PRODUCTION OF OXYGENATED COMPOUNDS FROM HEXOSES CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to U.S. Provisional Application No. 62/808,247 filed February 20, 2019, entitled “MICROORGANISMS AND METHODS FOR THE PRODUCTION OF OXYGENATED COMPOUNDS FROM HEXOSES”, the disclosures of which are incorporated by reference herein. TECHNICAL FIELD [0002] This application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol or monoethylene glycol and one or more co-product from one or more hexose feedstock. This application additionally relates to recombinant microorganisms useful in the biosynthesis of glycolic acid or glycolic acid and one or more co-product from one or more hexose feedstock. The application further relates to methods of producing monoethylene glycol or monoethylene glycol and one or more co-product from one or more hexose feedstock using the recombinant microorganisms, as well as methods of producing glycolic acid or glycolic acid and one or more co-product from one or more hexose feedstock using the recombinant microorganisms. The application further relates to compositions comprising one or more of these compounds and/or the recombinant microorganisms. STATEMENT REGARDING SEQUENCE LISTING [0003] The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is BRSK-004_02WO_ST25.txt. The text file is about 616 KB, was created on February 18, 2020, and is being submitted electronically via EFS-Web. BACKGROUND [0004] A large number of chemical compounds are currently derived from petrochemicals. Compounds such as monoethylene glycol (MEG), glycolic acid, acetone, isopropanol ( IPA), propene, serine, glycine, monoethanolamine, and ethylenediamine are valuable as raw material in the production of products like polyethylene terephthalate (PET) resins (from MEG), plastic polypropylene (from propene), polyglycolic acid and other biocompatible copolymers (from glycolic acid) and polyurethane fibers (from ethylenediamine). Alkenes (such as ethylene, propylene, different butenes, and pentenes, for example) are used in the plastics industry, fuels, and in other areas of the chemical industry. For example, isobutene is a small, highly reactive molecule that is used extensively as a platform chemical to manufacture a wide variety of products including fuel additives, rubber and rubber additives, and specialty chemicals. [0005] However, the compounds are currently produced from precursors that originate from fossil fuels, which contribute to climate change. To develop more environmentally friendly processes for the production of MEG, researchers have engineered microorganisms with biosynthetic pathways to produce MEG. However, these pathways are challenging to implement, with loss of product yield, redox balance and excess biomass formation being some major obstacles to overcome. [0006] Thus there exists a need for improved biosynthesis pathways for the production of MEG and other chemical compounds useful in industrial and pharmaceutical applications. SUMMARY OF THE DISCLOSURE [0007] The present application relates to recombinant microorganisms having one or more biosynthesis pathways for the production of monoethylene glycol (MEG) or glycolic acid (GA), or optionally, MEG (or GA) and one or more co-product from one or more hexose feedstock. [0008] The recombinant microorganisms and methods of the present disclosure combine the advantages of glucose based, fermentative MEG production and xylose based, fermentative MEG production. In some embodiments, the recombinant microorganisms and methods of the present disclosure combine the advantages of xylose degradation biochemistry for high yielding MEG (or GA), or optionally, MEG (or GA) and one or more co product, formation with the advantages of readily available pure hexose sugar feedstocks. [0009] In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of xylose feedstock availability. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of non-affordable xylose feedstock price. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of xylose feedstock impurities. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of inefficient xylose uptake by a microorganism. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of glucose induced inhibition of xylose utilization. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of a shortage of ATP in MEG (or GA) production pathways. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of excess NADH in MEG (or GA) production pathways. In some embodiments, the recombinant microorganisms and methods of the present disclosure solves the problem of low overall product yield potential. [0010] In some embodiments, the recombinant microorganisms and methods of the present disclosure provide a lossless conversion of one or more hexose feedstock to one or more pentose- 5-phosphate intermediate. In some embodiments, the one or more pentose-5- phosphate intermediate is used for the production of MEG (or GA), or optionally, MEG (or GA) and one or more co-product, by one or more xylose based fermentation methods. In some embodiments, glucose flux is funneled into the pentose phosphate pathway instead of the glycolysis pathway. [001 1] In one aspect, the present disclosure provides a recombinant microorganism comprising one or more biochemical pathway that produces monoethylene glycol (MEG) (or glycolic acid) from one or more hexose feedstock via one or more pentose-5-phosphate intermediate. In one embodiment, one or more co-product is co-produced with MEG (or glycolic acid). In another embodiment, the one or more pentose- 5-phosphate intermediate is one or more of
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