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CDH12 Cadherin 12, Type 2 N-Cadherin 2 RPL5 Ribosomal

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5 6 6 5 . 4 2 1 1 1 2 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 A A A A A A A A A A A A A A A A A A A A C C C C C C C C C C C C C C C C C C C C R R R R R R R R R R R R R R R R R R R R B , B B B B B B B B B B B B B B B B B B B , 9 , , , , 4 , , 3 0 , , , , , , , , 6 2 , , 5 , 0 8 6 4 , 7 5 7 0 2 8 9 1 3 3 3 1 1 7 5 0 4 1 4 0 7 1 0 2 0 6 7 8 0 2 5 7 8 0 3 8 5 4 9 0 1 0 8 8 3 5 6 7 4 7 9 5 2 1 1 8 2 2 1 7 9 6 2 1 7 1 1 0 4 5 3 5 8 9 1 0 0 4 2 5 0 8 1 4 1 6 9 0 0 6 3 6 9 1 0 9 0 3 8 1 3 5 6 3 6 0 4 2 6 1 0 1 2 1 9 9 7 9 5 7 1 5 8 9 8 8 2 1 9 9 1 1 1 9 6 9 8 9 7 8 4 5 8 8 6 4 8 1 1 2 8 6 2 7 9 8 3 5 4 3 2 1 7 9 5 3 1 3 2 1 2 9 5 1 1 1 1 1 1 5 9 5 3 2 6 3 4 1 3 1 1 4 1 4 1 7 1 3 4 3 2 7 6 4 2 7 2 1 2 1 5 1 6 3 5 6 1 3 6 4 7 1 6 5 1 1 4 1 6 1 7 6 4 7 e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e e l l l l l l 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S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S CDH12 cadherin 12, type 2 N-cadherin 2 RPL5 ribosomal protein L5 RPL32 ribosomal protein L32 GNB2L1 guanine nucleotide binding protein G protein, beta polypeptide 2-like 1 RPS23 ribosomal protein S23 RPL27A ribosomal protein L27a ECE1 endothelin converting enzyme 1 LAMC2 laminin, gamma 2 GSTM2 glutathione S-transferase M2 muscle PLXNB1 plexin B1 RFC3 replication factor C activator 1 3, 38kDa AMFR autocrine motility factor receptor PXMP3 peroxisomal membrane protein 3, 35kDa Zellweger syndrome HSPBP1 hsp70-interacting protein HSPBP1 hsp70-interacting protein LIPH lipase, member H KIAA0429 KIAA0429 gene product KIAA0429 KIAA0429 gene product H1F2 H1 histone family, member 2 H2BFQ H2B histone family, member Q FLJ10607 hypothetical protein FLJ10607 similar to glucosamine-phosphate N-acetyltransferase CEACAM6 carcinoembryonic antigen-related cell adhesion molecule 6 non-specific cross reacting antigen PTPRN2 protein tyrosine phosphatase, receptor type, N polypeptide 2 PCSK1 proprotein convertase subtilisin/kexin type 1 CHGB chromogranin B secretogranin 1 CKMT1 creatine kinase, mitochondrial 1 ubiquitous CKMT1 creatine kinase, mitochondrial 1 ubiquitous CA11 carbonic anhydrase XI CA2 carbonic anhydrase II Ells1 hypothetical protein Ells1 STK19 serine/threonine kinase 19 BECN1 beclin 1 coiled-coil, myosin-like BCL2 interacting protein ATOX1 ATX1 antioxidant protein 1 homolog yeast CRABP2 cellular retinoic acid binding protein 2 GTF2H2 general transcription factor IIH, polypeptide 2, 44kDa CYP2A6 cytochrome P450, subfamily IIA phenobarbital-inducible, polypeptide 6 WFDC2 WAP four-disulfide core domain 2 C20orf55 chromosome 20 open reading frame 55 APOD apolipoprotein D HMGCS2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 mitochondrial FLJ10948 hypothetical protein FLJ10948 FLJ10509 hypothetical protein FLJ10509 C9orf12 chromosome 9 open reading frame 12 C9orf12 chromosome 9 open reading frame 12 PBP prostatic binding protein FLJ20452 hypothetical protein FLJ20452 SIAH2 seven in absentia homolog 2 Drosophila NME4 non-metastatic cells 4, protein expressed in MB myoglobin SLC20A1 solute carrier family 20 phosphate transporter, member 1 KIAA0222 KIAA0222 gene product DUSP16 dual specificity phosphatase 16 DUSP16 dual specificity phosphatase 16 DKFZP564L2423 hypothetical protein DKFZp564L2423 ALDH3A2 aldehyde dehydrogenase 3 family, member A2 DKFZp566O084 DKFZP566O084 protein GRIA2 glutamate receptor, ionotropic, AMPA 2 AGTR1 angiotensin II receptor, type 1 AGTR1 angiotensin II receptor, type 1 AGTR1 angiotensin II receptor, type 1 H11 protein kinase H11 ENPP5 ectonucleotide pyrophosphatase/phosphodiesterase 5 putative function HRASLS3 HRAS-like suppressor 3 LAMA5 laminin, alpha 5 RAB2L RAB2, member RAS oncogene family-like ACOX2 acyl-Coenzyme A oxidase 2, branched chain ATP6V1G1 ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 1 FLJ38045 hypothetical protein FLJ38045 BLVRA biliverdin reductase A IGFBP2 insulin-like growth factor binding protein 2, 36kDa IGFBP2 insulin-like growth factor binding protein 2, 36kDa DXS1283E GS2 gene AKR7A2 aldo-keto reductase family 7, member A2 aflatoxin aldehyde reductase QDPR quinoid dihydropteridine reductase ShrmL Shroom-related protein MGST2 microsomal glutathione S-transferase 2 PLAT plasminogen activator, tissue PLAT plasminogen activator, tissue TFF1 trefoil factor 1 breast cancer, estrogen-inducible sequence expressed in MSX2 msh homeo box homolog 2 Drosophila SELENBP1 selenium binding protein 1 DKFZp761F2014 hypothetical protein DKFZp761F2014 BECN1 beclin 1 coiled-coil, myosin-like BCL2 interacting protein ASAH1 N-acylsphingosine amidohydrolase acid ceramidase 1 GLUD1 glutamate dehydrogenase 1 CYB5 cytochrome b-5 KIAA0303 KIAA0303 protein KIAA0303 KIAA0303 protein IRS1 insulin receptor substrate 1 RERG RAS-like, estrogen-regulated, growth-inhibitor LIV-1 LIV-1 protein, estrogen regulated COX6C cytochrome c oxidase subunit VIc KIAA1025 KIAA1025 protein PTP4A2 protein tyrosine phosphatase type IVA, member 2 NAT1 N-acetyltransferase 1 arylamine N-acetyltransferase SLC11A3 solute carrier family 11 proton-coupled divalent metal ion transporters, member 3 PRO1489 hypothetical protein PRO1489 APBB2 amyloid beta A4 precursor protein-binding, family B, member 2 Fe65-like HIS1 HMBA-inducible FLJ13322 hypothetical protein FLJ13322 KIAA0876 KIAA0876 protein ACADSB acyl-Coenzyme A dehydrogenase, short/branched chain ACADSB acyl-Coenzyme A dehydrogenase, short/branched chain INPP4B inositol polyphosphate-4-phosphatase, type II, 105kDa TCEAL1 transcription elongation factor A SII-like 1 FBP1 fructose-1,6-bisphosphatase 1 XBP1 X-box binding protein 1 GATA3 GATA binding protein 3 ESR1 estrogen receptor 1 VAV3 vav 3 oncogene DIP13B DIP13 beta D5S346 DNA segment, single copy probe LNS-CAI/LNS-CAII deleted in polyposis CEGP1 CEGP1 protein LRBA LPS-responsive vesicle trafficking, beach and anchor containing GSTM3 glutathione S-transferase M3 brain C4B complement component 4B BC008967 hypothetical gene BC008967 IGBP1 immunoglobulin CD79A binding protein 1 Homo sapiens cDNA FLJ40165 fis, clone TESTI2015962 KIAA1243 KIAA1243 protein HSD17B4 hydroxysteroid 17-beta dehydrogenase 4 DUSP4 dual specificity phosphatase 4 RNASE4 ribonuclease, RNase A family, 4 SCNN1A sodium channel, nonvoltage-gated 1 alpha PTPRT protein tyrosine phosphatase, receptor type, T FLJ25604 hypothetical protein FLJ25604 EIF4G3 eukaryotic translation initiation factor 4 gamma, 3 KIAA0310 KIAA0310 gene product SEMA3C sema domain, immunoglobulin domain Ig, short basic domain, secreted, semaphorin 3C KIAA0239 KIAA0239 protein BF B-factor, properdin RARRES3 retinoic acid receptor responder tazarotene induced 3 BST2 bone marrow stromal cell antigen 2 CRAT carnitine acetyltransferase CRAT carnitine acetyltransferase TFAP2B transcription factor AP-2 beta activating enhancer binding protein 2 beta MUC1 mucin 1, transmembrane PDCD4 programmed cell death 4 neoplastic transformation inhibitor PDCD4 programmed cell death 4 neoplastic transformation inhibitor SLI neuronal Shc adaptor homolog SNK serum-inducible kinase FLNB filamin B, beta actin binding protein 278 FLJ11730 hypothetical protein FLJ11730 FLJ11730 hypothetical protein FLJ11730 NQO1 NADPH dehydrogenase, quinone 1 RGS5 regulator of G-protein signalling 5 ABCD3 ATP-binding cassette, sub-family D ALD, member 3 FMO5 flavin containing monooxygenase 5 DKFZP564D0372 hypothetical protein DKFZp564D0372 GPX4 glutathione peroxidase 4 phospholipid hydroperoxidase RALGPS1A Ral guanine nucleotide exchange factor RalGPS1A EPHX2 epoxide hydrolase 2, cytoplasmic KIAA0857 KIAA0857 protein EPB72 erythrocyte membrane protein band 7.2 stomatin LOC51092 CGI-40 protein ATP6V1B1 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1 Renal tubular acidosis with deafness NPY1R neuropeptide Y receptor Y1 CCND1 cyclin D1 PRAD1: parathyroid adenomatosis 1 CCND1 cyclin D1 PRAD1: parathyroid adenomatosis 1 FADD Fas TNFRSF6-associated via death domain ZNF220 zinc finger protein 220 TRPS1 trichorhinophalangeal syndrome I IL18BP interleukin 18 binding protein EPOR erythropoietin receptor RAD52 RAD52 homolog S.
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  • Protein Identities in Evs Isolated from U87-MG GBM Cells As Determined by NG LC-MS/MS

    Protein Identities in Evs Isolated from U87-MG GBM Cells As Determined by NG LC-MS/MS

    Protein identities in EVs isolated from U87-MG GBM cells as determined by NG LC-MS/MS. No. Accession Description Σ Coverage Σ# Proteins Σ# Unique Peptides Σ# Peptides Σ# PSMs # AAs MW [kDa] calc. pI 1 A8MS94 Putative golgin subfamily A member 2-like protein 5 OS=Homo sapiens PE=5 SV=2 - [GG2L5_HUMAN] 100 1 1 7 88 110 12,03704523 5,681152344 2 P60660 Myosin light polypeptide 6 OS=Homo sapiens GN=MYL6 PE=1 SV=2 - [MYL6_HUMAN] 100 3 5 17 173 151 16,91913397 4,652832031 3 Q6ZYL4 General transcription factor IIH subunit 5 OS=Homo sapiens GN=GTF2H5 PE=1 SV=1 - [TF2H5_HUMAN] 98,59 1 1 4 13 71 8,048185945 4,652832031 4 P60709 Actin, cytoplasmic 1 OS=Homo sapiens GN=ACTB PE=1 SV=1 - [ACTB_HUMAN] 97,6 5 5 35 917 375 41,70973209 5,478027344 5 P13489 Ribonuclease inhibitor OS=Homo sapiens GN=RNH1 PE=1 SV=2 - [RINI_HUMAN] 96,75 1 12 37 173 461 49,94108966 4,817871094 6 P09382 Galectin-1 OS=Homo sapiens GN=LGALS1 PE=1 SV=2 - [LEG1_HUMAN] 96,3 1 7 14 283 135 14,70620005 5,503417969 7 P60174 Triosephosphate isomerase OS=Homo sapiens GN=TPI1 PE=1 SV=3 - [TPIS_HUMAN] 95,1 3 16 25 375 286 30,77169764 5,922363281 8 P04406 Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3 - [G3P_HUMAN] 94,63 2 13 31 509 335 36,03039959 8,455566406 9 Q15185 Prostaglandin E synthase 3 OS=Homo sapiens GN=PTGES3 PE=1 SV=1 - [TEBP_HUMAN] 93,13 1 5 12 74 160 18,68541938 4,538574219 10 P09417 Dihydropteridine reductase OS=Homo sapiens GN=QDPR PE=1 SV=2 - [DHPR_HUMAN] 93,03 1 1 17 69 244 25,77302971 7,371582031 11 P01911 HLA class II histocompatibility antigen,
  • Characterisation of Bilirubin Metabolic Pathway in Hepatic Mitochondria Siti Nur Fadzilah Muhsain M.Sc

    Characterisation of Bilirubin Metabolic Pathway in Hepatic Mitochondria Siti Nur Fadzilah Muhsain M.Sc

    Characterisation of Bilirubin Metabolic Pathway in Hepatic Mitochondria Siti Nur Fadzilah Muhsain M.Sc. (Medical Research) 2005 Universiti Sains Malaysia Postgrad. Dip. (Toxicology) 2003 University of Surrey B.Sc.(Biomed. Sc.) 2000 Universiti Putra Malaysia A thesis submitted for the degree of Doctor of Philosophy at The University of Queensland in 2014 School of Medicine ABSTRACT Bilirubin (BR), a toxic waste product of degraded haem, is a potent antioxidant at physiological concentrations. To achieve the maximum benefit of BR, its intracellular level needs to be carefully regulated. A system comprising of two enzymes, haem oxygenase-1 (HMOX1) and cytochrome P450 2A5 (CYP2A5) exists in the endoplasmic reticulum (ER), responsible for regulating BR homeostasis. This system is induced in response to oxidative stress. In this thesis, oxidative stress caused accumulation of these enzymes in mitochondria — major producers and targets of reactive oxygen species (ROS) — is demonstrated. To understand the significance of this intracellular targeting, properties of microsomal and mitochondrial BR metabolising enzymes were compared and the capacity of mitochondrial CYP2A5 to oxidise BR in response to oxidative stress is reported. Microsomes and mitochondrial fractions were isolated from liver homogenates of DBA/2J mice, administered with sub-toxic dose of pyrazole, an oxidant stressor. The purity of extracted organelles was determined by analysing the expressions and activities of their respective marker enzymes. HMOX1 and CYP2A5 were significantly increased in microsomes and even more so in mitochondria in response to pyrazole-induced oxidative stress. By contrast, the treatment did not increase either microsomes or mitochondrial Uridine-diphosphate-glucuronosyltransferase 1A1 (UGT1A1), the sole enzyme that catalyses BR elimination through glucuronidation.