The complete portfolio for your B cell research Product quick guide Human B cells from whole blood and bone marrow
Whole blood Buffy coat Leukopak LRSC Density gradient
StraightFrom® Whole Blood StraightFrom Buffy Coat StraightFrom Leukopak StraightFrom LRSC PBMCs CD19 MicroBead Kit CD19 MicroBead Kit CD19 MicroBead Kit CD19 MicroBead Kit StraightFrom Whole Blood MACSxpress Buffy Coat MACSxpress LRSC Pan CD45 MicroBead Kit Pan B Cell Isolation Kit B Cell Isolation Kit MACSxpress® Whole Blood Pan B Cell Isolation Kit CD45+ MACSxpress Whole Blood CD14+/− B Cell Isolation Kit CD15+/− HLA-DR+ CD3+/− CD19+/− CD56+/−
MACSxpress StraightFrom MACSxpress B Cell Isolation Naive B Cell Pre-selection CD138 Memory B Cell Anti-ROR1 Whole Blood Whole Blood Whole Blood Kit II Isolation Kit II with: MicroBeads Isolation Kit MicroBead Kit Naive B Cell CD138 B-CLL Cell Pan B Cell CD43 MicroBeads B Cell Isolation CD38 IgM+ Memory B CD138 Isolation Kit MicroBeads Isolation Kit Isolation Kit Anti-IgD Kit II MicroBead Kit Cell Isolation Kit MicroBeads StraightFrom CD19 MicroBeads MicroBeads + Plasma Cell IgG+ Memory B B-CLL Cell Whole Blood CD25 Isolation Kit II Cell Isolation Kit Isolation Kit Anti-ROR1 CD19 MultiSort MicroBeads II MicroBead Kit Kit or Switched CD10 CD20 MicroBeads CD30 Memory B Cell MicroBead Kit Isolation Kit CD22 MicroBeads MicroBeads or CD27 REAlease® CD19 CD69 MicroBead MicroBeads MicroBead Kit Kit II Anti-IgG MicroBeads Anti-IgM MicroBeads
Naive Plasma Abnormal Pan Naive Activated Plasma Memory Abnormal B cells cells B cells B cells B cells B cells cells B cells B cells
CD19+ CD19low CD19+ CD19+ CD19+ CD19+ CD19low CD19+ CD19+ CD20+ CD20− CD20+ CD20+ CD20+ CD20+ CD20− CD20+ CD20+ CD27− CD138+ ROR1+ CD22+ CD27− CD27+ CD138+ CD27+/− ROR1+ CD22+ CD38high CD5+ Ig+/− CD22+ CD30+ CD38high CD38− CD5+ IgD+ CD27+ Ig+/− IgD+ CD25+/− CD27+ CD40+ CD10+ MHCI class CD69+ MHCI class CD23low Ig+/− II+/− CD80/86+ II+/− IgM/A/E/ Ig− IgM+ Ig− G+ IgD+ IgD+/−
Pre-enrichment with: Pre-selection with: CD138 MicroBeads Anti-ROR1 MicroBead Kit StraightFrom CD10 MicroBead Kit B Cell Isolation Kit II CD38 MicroBead Kit CD138 MicroBeads Whole Blood and or Bone Marrow CD138 REAlease CD19 Plasma Cell Isolation Kit II B-CLL Cell Isolation Kit MicroBeads MicroBead Kit + CD10 MicroBead Kit
Pro B cells Pre and/or immature B cells Plasma cells Abnormal CLL B cells Plasma cells
CD10+ CD10+ CD19low CD19+ CD19low CD19+ CD19+ CD20− CD20+ CD20− CD38+ CD20+ CD138+ ROR1+ CD138+ CD34+ CD38+ CD38high CD5+ CD38high CD24+ CD21+ CD27+ Ig+/− CD27+ IgM− CD24+ MHCI class MHCI class IgM+/− II+/− II+/− IgD− Ig− Ig−
B cell cultivation and activation
B cells and B cell subsets Expanded and activated B cells
B Cell Expansion Kit, TLR Ligands* Cytokines e.g., IL-4*, IL-21, TNF-α* CD19+ Human CD40-Ligand Multimer Kits CD19+ CD30+ CD20+ CD20+ CD25+ CD22+ CD22+ MHC class Ig+/− CD80/86+ IIhigh CD69+ Ig+/−
Legend
direct cell separation products CLL: chronic lymphocytic leukemia – no expression separation ROR: receptor tyrosine kinase-like orphan receptor 1 + expression differentiation/cultivation LRSC: leukocyte reduction system chamber low low expression PBMC: peripheral blood mononuclear cells int intermediate expression MACS® Cell Separation products (RUO) HLA: human leukocyte antigen high high expression MACS Cell Culture and Stimulation products TNF: tumor necrosis factor +/– expression dependent on subset MACS Flow Cytometry and Cytokine CD: cluster of differentiation detection products (RUO) MHC: major histocompatibility complex *Available also as MACS GMP product Ig: immunoglobulin
3 Human Product tables
Cell separation reagents Cell culture reagents
Products Order no. Products Research Premium MACS® grade grade GMP Anti-IgD MicroBeads 130-103-775 BAFF ● Anti-IgG MicroBeads 130-047-501 B Cell Expansion Kit ● Anti-IgM MicroBeads 130-093-230 CD40-Ligand Multimer Kit ● Anti-ROR1 MicroBead Kit 130-103-929 CXCL13 ● B Cell Isolation Kit II 130-091-151 Flt3 Ligand ● ● ● B-CLL Cell Isolation Kit 130-103-466 IFN-γ1b ● ● CD10 MicroBead Kit 130-093-452 IL1-beta ● ● ● CD19 MicroBeads 130-050-301 ● ● ● CD19 MultiSort Kit 130-055-301 IL-2 improved sequence (IS) ● ● ● CD20 MicroBeads 130-091-104 IL-3 ● ● ● CD22 MicroBeads 130-046-401 IL-4 CD27 MicroBeads 130-051-601 IL-6 ● ● ● CD38 MicroBead Kit 130-092-263 IL-7 ● ● ● CD43 MicroBeads 130-091-333 IL-10 ● CD138 MicroBeads 130-051-301 IL-12 ● IgG+ Memory B Cell Isolation Kit 130-094-350 IL-13 ● ● IgM+ Memory B Cell Isolation Kit 130-093-619 IL-17 ● Memory B Cell Isolation Kit 130-093-546 IL-21 ● ● ● Naive B Cell Isolation Kit II 130-091-150 IL-35 ● Pan B Cell Isolation Kit 130-101-638 PepTivator® (Peptide Pools) ● ● ● Plasma Cell Isolation Kit II 130-093-628 SCF ● ● ● Switched Memory B Cell Isolation Kit 130-093-617 SDF-1α ● TGF-β1 ● ● REAlease® TLR7-Ligands ● REAlease CD19 MicroBead Kit 130-117-034 TLR9-Ligands ● ● StraightFrom® TNF-α ● ● ● StraightFrom Buffy Coat CD19 MicroBead Kit 130-114-974 TSLP ● StraightFrom Leukopak® CD19 MicroBead Kit 130-117-021 StraightFrom LRSC CD19 MicroBead Kit 130-117-025 StraightFrom Whole Blood CD19 MicroBeads 130-090-880 For more information about custom fillings and StraightFrom Whole Blood CD138 MicroBeads 130-093-062 cocktails visit: StraightFrom Whole Blood and Bone Marrow 130-105-961 www.miltenyibiotec.com/cellculture CD138 MicroBeads StraightFrom Whole Blood Anti-ROR1 130-105-751 MicroBead Kit
MACSxpress® MACSxpress Buffy Coat Pan B Cell Isolation Kit 130-120-002 MACSxpress LRSC Pan B Cell Isolation Kit 130-119-998 MACSxpress Whole Blood B Cell Isolation Kit 130-098-190 MACSxpress Whole Blood B-CLL Cell Isolation Kit 130-104-445 MACSxpress Whole Blood Naive B Cell Isolation Kit 130-098-186 MACSxpress Whole Blood Pan B Cell Isolation Kit 130-104-444
4 Flow cytometry antibodies Flow cytometry cocktails and kits
Antigen Hybridoma REAfinity™ REAlease® Products Order no. Clones Clones Clones GM-CSF Secretion Assay – Detection Kit (PE) 130-105-755 CD1d 51.1 IFN-γ Secretion Assay – Detection Kit (APC) 130-090-762 CD5 UCHT2 REA782 REAL382 IFN-γ Secretion Assay – Detection Kit (FITC) 130-090-433 CD10 97C5 REA877 REAL318 IFN-γ Secretion Assay – Detection Kit (PE) 130-054-202 CD19 LT19 REA675 REAL106 IL-2 Secretion Assay – Detection Kit (APC) 130-090-763 CD20 LT20 REA780 IL-2 Secretion Assay – Detection Kit (PE) 130-090-487 CD21 HB5 REA940 IL-4 Secretion Assay – Detection Kit (PE) 130-054-102 CD22 REA340 IL-5 Secretion Assay – Detection Kit (APC) 130-091-624 CD23 M-L23.4 REA1222 IL-5 Secretion Assay – Detection Kit (PE) 130-091-623 CD24 32D12 REA832 IL-10 Secretion Assay – Detection Kit (APC) 130-090-761 CD25 4E3, 3G10 REA570, REAL128 IL-10 Secretion Assay – Detection Kit (PE) 130-090-434 REA945 IL-12 Secretion Assay – Cell Enrichment and 130-092-122 CD27 M-T271, REA499 REAL173 Detection Kit (PE) LG.3A10 RCI Anti-IL-2-PE 130-099-450 CD32 2E1 REA997 RCI Anti-IFN-y-APC 130-097-596 CD38 IB6 REA572, REA671 RCI Anti-IFN-γ-PE 130-097-600 CD43 DF-T1 REA833 TNF-α Secretion Assay – Detection Kit (APC) 130-091-267 CD45 5B1 REA747 REAL258 TNF-α Secretion Assay – Detection Kit (PE) 130-091-268 CD45RA T6D11 REA562, REA1047 CD45RB REA119 MACSPlex Custom Cytokine Assays CD45RO UCHL1 REA611 REAL377 CD80 2D10 REA661 Products Order no. CD86 FM95 REA968 MACSPlex Cytokine Basic Kit, human and mouse 130-109-701 CD93 VIMD2 REA1111 MACSPlex Cytokine 12 Kit 130-099-169 CD95 (FAS) DX2 REA738 REAL186 MACSPlex GM-CSF Reagents Kit 130-109-684 CD138 44F9 MACSPlex IFN-α Reagents Kit 130-109-683 IL-10 JES3-9D7 REA842 MACSPlex IFN-γ Reagents Kit 130-109-695 IgD IgD26 REA740 MACSPlex IL-2 Reagents Kit 130-109-704 IgM PJ2-22H3 MACSPlex IL-4 Reagents Kit 130-109-689 IgG IS11-3B2.2.3 MACSPlex IL-5 Reagents Kit 130-109-705 IgG (H+L) Polyclonal MACSPlex IL-6 Reagents Kit 130-109-691 IgG1 IS11- MACSPlex IL-9 Reagents Kit 130-109-693 12E4.23.20 MACSPlex IL-10 Reagents Kit 130-109-699 IgE MB10-5C4 REA1049 MACSPlex IL-12 Reagents Kit 130-109-697 IgA IS11-8E10 REA1014 MACSPlex IL-17A Reagents Kit 130-109-688 IgA2 IS11-21E11 REA995 MACSPlex TNF-α Reagents Kit 130-109-694 HLA Many different clones to several HLA class antigens For more information about antibodies and fluorescent conjugates for flow cytometry visit: www.miltenyibiotec.com/flowcytometry
5 Mouse B cells from bone marrow, spleen, and lymphoid tissue
Bone marrow
Pre-selection with: Pre-enrichment Pre-selection Pre-enrichment CD138 Pan B cell Pan B Cell Isolation with: with: with: MicroBeads Isolation Kit II Kit II Pan B Cell B cell Isolation B Cell Isolation Kit + Isolation Kit II Kit CD138+ Plasma CD43 (Ly-48) + Cell Isolation Kit MicroBeads CD93 MicroBeads
Pro B cells Pre B cells Immature B cells Plasmablasts Plasma cells Abnormal B cells
CD19+ CD19+ CD19+ CD19+ CD138+ CD19+ CD43+ CD43low CD93+ CD138+ CD38low CD20+ CD20+/− CD20+ CD43− CD45R(B220)low CD19 − CD5+ CD24+ CD24+ CD23− MHC class II+ CD45R(B220)low Ig+/− CD45R(B220)+ CD25+/− CD20+ IgM/A/E/G+ CD93+/− IgM− CD45R(B220)+ CD24+ MHC class II+/− IgM− CD45R(B220)+ Ig− IgM+ IgD−
6 Spleen/lymphoid tissue Body cavities
Pre-selection MZ and Regulatory Germinal Pre- CD138 Memory B Cell Pan B cell B-1a Cell B-1a Cell with: FO B Cell B Cell Center B enrichment MicroBeads Isolation Kit Isolation Kit II Isolation Kit Isolation Kit B Cell Isolation Kit Isolation Cell (PNA) with: CD138+ CD5 (Ly-1) Kit MicroBead Anti-Mouse B Cell Isolation Isolation Kit CD23 B Cell Plasma Cell IgG1 Kit MicroBeads flow through MicroBeads Kit Isolation Kit Isolation Kit + CD93 MicroBeads CD19 of second MicroBeads Anti-Mouse MicroBeads magnetic selection IgG2a+b CD22 (CD5− cells) MicroBeads MicroBeads Anti- CD23 Mouse IgM MicroBeads MicroBeads CD45R (B220) MicroBeads
Transitional MZ and Regulatory Germinal Plasmablasts Plasma Memory Pan B cells B1a cells B1b cells B cells FO B cells B cells center cells cells B cells
CD19+ CD19+ CD19+ PNA+ CD19+ CD138+ CD19+ CD19+ CD19+ CD19+ CD24+ CD21+/− CD5+ CD19+ CD138+ CD38low CD20+ CD20+ CD45R(B220)+ CD45R(B220)+ CD93+ CD22+ CD24+ CD38low CD45R(B220)low CD19 − CD22+ CD22+ CD5+ CD5− CD21+/− CD23+/− CD1dhigh CD20+ MHC class II+ CD45R(B220)low CD38+/− CD23+/− CD11b + CD11b + CD45R(B220)+ CD93− CD93+/− CD93− IgM/A/E/G+ CD93+/− CD45R(B220)+ CD45R(B220)+ CD43+ CD43+ CD23+/− CD35+/− IL10, GL7+ MHC class II+/− CD95low CD45+ CD23− CD23− IgMhigh CD45R(B220)+ TGFß CD37+ Ig− CD80+/− Ig+/− IgMhigh IgMhigh IgDlow IgM+/− secretion IgM/A/E/G+ IgM/A/E/G+ IgDlow IgDlow IgD+/− IgMhigh IgD+/− IgD+/− IgD+/−
Legend
separation MZ: marginal zone – no expression FO: follicular low low expression MACS® Cell Separation products (RUO) TGF: transforming growth factor int intermediate expression MACS Flow Cytometry and Cytokine + detection products (RUO) CD: cluster of diff erentiation expression MHC: major histocompatibility complex high high expression Ig: immunoglobulin +/– expression dependent on subset PNA: peanut agglutinin
7 Mouse Product tables
Cell separation reagents Cell culture reagents
Products Order no. Products Research Premium Functional grade grade grade Anti-Mouse IgG1 MicroBeads 130-047-101 Flt3 Ligand ● Anti-Mouse IgG2a+b MicroBeads 130-047-201 IFN-γ ● ● Anti-Mouse IgM MicroBeads 130-047-301 IL-4 ● ● B-1a Cell Isolation Kit 130-097-413 IL-5 ● B Cell Isolation Kit 130-090-862 IL-6 ● ● CD5 (Ly-1) MicroBeads 130-049-301 IL-13 ● CD19 MicroBeads 130-121-301 SCF ● ● CD22 MicroBeads 130-103-776 CD23 MicroBeads 130-098-784 Stimulation antibodies CD43 (Ly-48) MicroBeads 130-049-801 CD2 antibody pure ● CD45R (B220) MicroBeads 130-049-501 CD48 antibody pure ● CD93 MicroBeads 130-105-753 CD127 antibody pure ● CD138 MicroBeads 130-098-257 CD134 (OX40) ● antibody pure CD138+ Plasma Cell Isolation Kit 130-092-530 CD137 antibody pure ● Germinal Center B Cell (PNA) MicroBead Kit 130-110-479 CD153 antibody pure ● Memory B Cell Isolation Kit 130-095-838 CD252 (OX40L) ● MZ and FO B Cell Isolation Kit 130-100-366 antibody pure Pan B Cell Isolation Kit II 130-104-443 CD275 (B7-H2) ● Pan B Cell Isolation Kit 130-095-813 antibody pure Regulatory B Cell Isolation Kit 130-095-873 CD279 (PD1) ● antibody pure
8 Flow cytometry antibodies Flow cytometry cocktails and kits
Antigen Hybridoma REAfinity™ REAlease® Products Order no. Clones Clones Clones Mouse IL-2 Secretion Assay – Detection Kit (APC) 130-090-987 CD1d 1B1 Mouse IL-2 Secretion Assay – Detection Kit (PE) 130-090-491 CD5 53-7.3 REA421 REAL174 Mouse IL-4 Secretion Assay – Detection Kit (PE) 130-090-479 CD11b M1/70.15.11.5 REA592 REAL113 Mouse IL-5 Secretion Assay – Detection Kit (APC) 130-091-174 CD19 6D5 REA749 Mouse IL-5 Secretion Assay – Detection Kit (PE) 130-091-166 CD21/CD35 7E9 REA800 Mouse IL-10 Secretion Assay – Detection Kit (APC) 130-090-939 CD22 Cy34.1 REA1187 Mouse IL-10 Secretion Assay – Detection Kit (PE) 130-090-489 CD23 B3B4 REA1068 Mouse IFN-γ Secretion Assay – Detection Kit (APC) 130-090-984 CD24 M1/69 REA743 Mouse IFN-γ Secretion Assay – Detection Kit (PE) 130-090-516 CD25 7D4 REA568 CD27 LG.3A10 REA499 CD38 90.4 REA616 REAL181 MACSPlex Custom Cytokine Assays CD43 L11 REA840 CD45 30F11 REA737 REAL180 Products Order no. CD45.1 A20 REA1179 MACSPlex Cytokine Basic Kit, human and mouse 130-109-701 CD45.2 104-2 REA1223 MACSPlex Cytokine 10 Kit 130-101-740 CD45R (B220) RA3-6B2 REA755 REAL132 MACSPlex GM-CSF Reagents Kit 130-109-702 CD45RA 14.8 REA639 MACSPlex IFN-γ Reagents Kit 130-109-687 CD45RB C363-16A REA1180 MACSPlex IL-2 Reagents Kit 130-109-686 CD80 16-10A1 REA983 MACSPlex IL-4 Reagents Kit 130-109-690 CD86 PO3.3 REA1190 MACSPlex IL-5 Reagents Kit 130-109-692 CD93 REA298 MACSPlex IL-10 Reagents Kit 130-109-698 CD95 (FAS) REA453 MACSPlex IL-12 Reagents Kit 130-109-696 CD138 REA104 MACSPlex IL-17A Reagents Kit 130-109-685 IL-10 JES5-16E3 REA1008 MACSPlex IL-23 Reagents Kit 130-109-700 IgD 11-26c.2a REA772 MACSPlex TNF-α Reagents Kit 130-109-703 IgM X-54 REA979 IgG1 X-56 REA1017 For more information about antibodies and IgG2ab X-57 fluorescent conjugates for flow cytometry visit: MHC Class II M5/114.15.2 REA510 REAL362 www.miltenyibiotec.com/flowcytometry variants MHC Class II M5/114.15.2 REA528 REAL362 variants MHC Class II M5/114.15.2 REA564 REAL362 variants MHC Class II M5/114.15.2 REA610 REAL362 variants MHC Class II M5/114.15.2 REA813 REAL362 variants
9 Other species Product tables
Cell separation reagents Flow cytometry antibodies
Products Order no. Antigen Hybridoma Clones REAfinity™ Clones
Rat Rat Anti-Rat Kappa MicroBeads 130-047-401 CD5 REA750 CD43 MicroBeads 130-090-742 CD24 REA405 CD45R MicroBeads 130-090-495 CD32 REA256
Non-human primate CD38 REA683 CD20 MicroBeads 130-091-105 CD43 REA503 CD45 REA504 CD45R REA450 For more information about custom fillings and CD45RC REA1000 cocktails visit: CD86 24F www.miltenyibiotec.com/cellculture CD200 REA532 CD252 ATM-2 IgG1 RG11/39.2 IgG2a RG7/1.30 IgG2b RG7/11.1 MHC Class II REA510
Non-human primate CD45 MB4-6D6 REA1023
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