Shipra Vaishnava, Ph.D Assistant Professor Molecular Microbiology and Immunology 171 Meeting Street Brown University Providence RI 02912 Tel: 401-863-6403, E- mail: [email protected]

Education

The University of Georgia (UGA), Athens, GA Ph.D. Cellular Biology (December 2005) Maharaja Sayajirao University, Baroda, India M.S. Biotechnology (May 1999) Delhi University, India B.S. (Honors) Botany (June 1996)

Positions

2006- 2011 Post-doctoral fellow, UT Southwestern Medical Center at Dallas. 2011- 2014 Instructor, UT Southwestern Medical Center at Dallas. 2015- present Assistant Professor, Molecular Microbiology & Immunology, Brown University, Providence, Rhode Island.

Awards

2002 Burroughs-Wellcome Fund Student Travel Award 2004 American Society of Tropical Medicine and Hygiene Scientific Award 2011 Crohn’s and Colitis Foundation of America Young IBD Investigator Award 2017 Kavli Fellow, National Academy of Sciences

Publications

Completed: 1. White, M.W., Jerome, M.E., Vaishnava, S., Guerini, M., Behnke, M., and Striepen, B. (2005). Genetic rescue of a Toxoplasma gondii conditional cell cycle mutant. Molecular microbiology 55, 1060-1071. 2. Vaishnava, S., Morrison, D.P., Gaji, R.Y., Murray, J.M., Entzeroth, R., Howe, D.K., and Striepen, B. (2005). Plastid segregation and cell division in the apicomplexan parasite Sarcocystis neurona. Journal of cell science 118, 3397-3407. 3. Gaji, R.Y., Zhang, D., Breathnach, C.C., Vaishnava, S., Striepen, B., and Howe, D.K. (2006). Molecular genetic transfection of the coccidian parasite Sarcocystis neurona. Molecular and biochemical parasitology 150, 1-9. 4. Gubbels, M.J., Vaishnava, S., Boot, N., Dubremetz, J.F., and Striepen, B. (2006). A MORN- repeat protein is a dynamic component of the Toxoplasma gondii cell division apparatus. Journal of cell science 119, 2236-2245. 5. Vaishnava, S., and Striepen, B. (2006). The cell biology of secondary endosymbiosis--how parasites build, divide and segregate the apicoplast. Molecular microbiology 61, 1380-1387. 6. Vaishnava, S., and Hooper, L.V. (2007). Alkaline phosphatase: keeping the peace at the gut epithelial surface. Cell host & microbe 2, 365-367. 7. Vaishnava, S., Behrendt, C.L., Ismail, A.S., Eckmann, L., and Hooper, L.V. (2008). Paneth cells directly sense gut commensals and maintain homeostasis at the intestinal host-microbial interface. Proceedings of the National Academy of Sciences of the United States of America 105, 20858-20863. 8. Duerkop, B.A., Vaishnava, S., and Hooper, L.V. (2009). Immune responses to the microbiota at the intestinal mucosal surface. Immunity 31, 368-376. 9. Ismail, A.S., Severson, K.M., Vaishnava, S., Behrendt, C.L., Yu, X., Benjamin, J.L., Ruhn, K.A., Hou, B., DeFranco, A.L., Yarovinsky, F., et al. (2011). Gammadelta intraepithelial lymphocytes are essential mediators of host-microbial homeostasis at the intestinal mucosal surface. Proceedings of the National Academy of Sciences of the United States of America 108, 8743-8748. 10. Vaishnava, S., and Hooper, L.V. (2011). Eat your carrots! T cells are RARing to go. Immunity 34, 290-292. 11. Vaishnava, S., Yamamoto, M., Severson, K.M., Ruhn, K.A., Yu, X., Koren, O., Ley, R., Wakeland, E.K., and Hooper, L.V. (2011). The antibacterial lectin RegIIIgamma promotes the spatial segregation of microbiota and host in the intestine. Science 334, 255-258. 12. Reiff, S.B., Vaishnava, S., and Striepen, B. (2012). The HU protein is important for apicoplast genome maintenance and inheritance in Toxoplasma gondii. Eukaryotic cell 11, 905-915. 13. Raetz, M., Hwang, S.H., Wilhelm, C.L., Kirkland, D., Benson, A., Sturge, C.R., Mirpuri, J., Vaishnava, S., Hou, B., Defranco, A.L., et al. (2013). Parasite-induced TH1 cells and intestinal dysbiosis cooperate in IFN-gamma-dependent elimination of Paneth cells. Nature immunology 14, 136-142. 14. Derebe, M.G., Zlatkov, C.M., Gattu, S., Ruhn, K.A., Vaishnava, S., Diehl, G.E., MacMillan, J.B., Williams, N.S., and Hooper, L.V. (2014). Serum amyloid A is a retinol binding protein that transports retinol during bacterial infection. eLife 3, e03206. 15. Vaishnava, S. (2016). The Intestinal Mucus Layer Comes of Age. Trends in immunology 37, 3-4. 16. Iyer, N., and Vaishnava, S. (2016). Alcohol Lowers Your (Intestinal) Inhibitions. Cell host & microbe 19, 131-133. 17. Grizotte-Lake, M. and Vaishnava, S. (2018). Autophagy: Suicide prevention hotline for the gut epithelium. Cell host & microbe, 2018 Feb 14;23(2):147-148. 18. Grizotte-Lake, Zhong, G., M., Duncan K., Kirkwood, J., Iyer, N., Smolenski, I., Isoherranen, N. and Vaishnava, S.(2018). Gut commensals suppress epithelial cell retinoic acid synthesis to regulate intestinal IL-22 activity and prevent microbial dysbiosis. Immunity, 2018 Dec 18;49(6):1103-1115

Funding

Current: NIGMS/NIH, Centers of Biomedical Research Excellence (COBRE) [P20] - Project-002: Spatial and Functional Organization of Intestinal Microbiome, 05/01/16-02/28/19. Total cost $267,500/year. Direct cost $175,000/year.

U.S. Department of Defense, Discovery Award: Mapping gut microbiota composition in-situ using high-resolution spatial microdissection technique, 05/01/18-10/31/19, PI. Total cost $325,00/year. Direct cost $200,000/year.

NIDDK/NIH, R01 award: Role of epithelial cell intrinsic vitamin A metabolism in regulating immune function in the gut, 04/01/18-03/31/23, PI. Total cost $ 2,673,221. Direct cost $1,641,486.

Completed: Research Fellowship Award, Paneth cell specific innate immune response to intestinal bacteria. Crohn’s and colitis Foundation of America (CCFA), 2009-11. PI

Deans Award, Vaginal microbiome and HIV susceptibility, Brown University, 2015-2016, Co-PI

URI/COBRE Pilot Project Program, Role of vitamin A metabolism in immunity against intestinal bacteria, 06/2016 -10/2016, PI

Career Development Award, Bacteria Regulated Vitamin A metabolism in the intestine, CCFA, 01/2014-12/2016, PI.

Salomon Award, Brown University, Regulation of gut microbiome biogeography, 02/2016- 01/2017, PI

Invited Seminars 1. Gut Microbiota in Health and Disease Symposium held in Ecole Polytechnique Federale de Lausanne (EPFL), Switzerland 5/11. 2. Department of Pathology, Microbiology and Immunology, Vanderbilt University, TN, 1/13 3. Department of Immunology, Duke University, NC, 1/13 4. Molecular and Cell Biology Department, Berkeley, CA 1/13 5. Microbiology and Immunology Department, UCSF, CA, 2/13 6. Department of Molecular Biology, Princeton, NJ, 3/13 7. Department of Microbiology and Immunology, University of Tennessee, TN 4/13 8. Keynote speaker at Department of Cellular Biology Annual Retreat, University of Georgia, GA 08/13 9. Department of Biology, Carnegie Mellon University, PA, 12/13 10. Department of Molecular Microbiology, UC Davis, CA, 1/14 11. Molecular Microbiology and Immunology department, Brown University, RI, 2/14 12. Microbiology and Immunology Department, University of British Columbia, Canada, 3/14 13. Food Science Department, University of Nebraska, NE 3/14 14. School of Life Science, Ecole Polytechnique Federale de Lausanne (EPFL), Switzerland, 4/14 15. Department of Biology Seminar Series, Boston College, MA, 3/16 16. Pediatric Research Colloquium, Rhode Island Hospital, RI, 5/16 17. Mucosal Immunology Course and Symposium (MICS), Toronto, 8/16 18. EMBO Innate Lymphocyte Cell Symposium, Berlin, 11/16 19. Student invited seminar, Cell and Molecular Biology program, University of Rhode Island, 9/17 20. FASEB Nutritional Immunology Conference, Leesburg, VA 6/18

Teaching BIOL1250, Host-Microbiome Interactions in Health and Disease, Co-director, 2017- Spring, 2017: Student enrollment capped at 20. Total 12 lectures including 2 guest lecture. I taught 5 lectures, 3 hours per lecture. BIOL1600, Development of Vaccines to Infectious Diseases, Co-instructor, 2015- Spring, 2016, 2017: Student enrollment around 75. Total 21 lectures including 7 taught by guest or teaching assistant. I taught 5 lectures, 1 hour and 20 minutes per lecture. BIOL1520, Innate Immunity, Graduate/Undergraduate level course, Guest lecturer, 2015-. Fall, 2015, 2016, 2017: Student enrollment around 30. Taught 1 class per semester.

Service To the University Trainer in Pathobiology Graduate Program, 2015-present. Trainer in MCB Graduate Program, 2016-present. Biology undergraduate concentration advisor, Brown University, 2015-present. Pathobiology Graduate Student Admissions Committee, Brown University, 2015, 2017. Brown University Community Council, 2017-2020

To the profession Ad hoc reviewer for: Cell Host & Microbe Cell Metabolism Cell Stem Cell Elife Plos Pathogen Plos One

To the community Co-Chair, Mucosal Immunology Block-Symposium, AAI Meeting, Austin Texas, May, 2018.

Mentoring

Under Direct Supervision

Name Position Duration Joshua Hackney Undergraduate Researcher 2015-2016 Monica Thapliya Leadership Alliance Student 2015 Donald Brennan Undergraduate Researcher 2016 Kelly Carey-Erwend Undergraduate Researcher 2016-present Douglas Wu Undergraduate Researcher 2017-present Kellyanne Duncan Graduate Student 2015-present Mayara Grizotte-Lake Graduate Student 2015-present Namrata Iyer Post-doctoral Fellow 2015-present Sarah Gordon Graduate Student 2017-present

In Advisory Function:

Name Period Advisor Advisory Role Ryan Xu 2015-2018 Amanda Jamieson Thesis committee member Jenna Perry 2015- Chris de Graffenried Thesis committee member Aislinn Rowan 2016- Peter Belenky Thesis committee member Gregory Serpa 2017- Amanda Jamieson Thesis committee chair Gregory Thomson 2017- Richard Bennett Thesis committee member Angela Tata 2017- Laurent Brossay Thesis committee chair Jenna Wurster 2018- Peter Belenky Thesis committee member Michelle Zabat 2017 Peter Belenky Honors thesis second reader Zemplen Pataki 2017 Chris de Graffenried Honors thesis second reader