Bio 112L General Biology I Laboratory Fall 2012
INSTRUCTORS and LAB SECTIONS—all labs meet in Folsom 304 Robert Pinette 0004 (8606) Tu 2:00-4:45 [email protected] Judy Roe 0005 (8607) Th 2:00-4:45 [email protected] Rachael Hannah 0007 (8609) We 2:00-4:45 [email protected] “ “ 0008 (8610) Mo 12:30-3:15 “ “
LAB MANUAL:
PINETTE, R. 2012. A LABORATORY MANUAL FOR GENERAL BIOLOGY 112. 10th ed. Northern Products and Printers, Presque Isle, ME. 82 pp.
I.COURSE OUTLINE. Lab meets once weekly in Folsom 304. See above for instructors, laboratory meeting times and contact information. Week of: TOPIC TESTS AND ASSIGNMENTS Sep 3 Labor Day--No Labs This Week Course Introduction Lab Safety & Etiquette Review the units of measurement and do the Sep 10 metric problems on pages 9 & 10 before 2.System of International Units & coming to lab Dissecting Microscope (pp 9,11-12) Quiz#1 on Safety, Etiquette, SI 1. The Compound Microscope System & Dissecting Microscope 2. Making a Drawing of Amoeba Sep 17 Drawing of an Amoeba cell. Before from a Prepared Slide (Part IV) lab read instructions for making drawings— pages iii- iv. Microscope Calibration and Cell Quiz#2 on Ex 1 & 2 Sep 24 Measurements (pp. v-vi and 10) Plant and animal cells drawings 3. Plant and Animal Cells Quiz#3 on Ex 3 & Microscope Calibration Oct 1 4. Osmosis Elodea Cell Drawings/Osmosis Report Assigned Oct 8 Fall Break—No Labs This Week 5. Alcohol Fermentation Osmosis Report Due Oct 15
Quiz#4 on 4 & 5 Oct 22 6. Mitosis Drawings of mitosis figures Oct 29 10. Enzyme Activity Enzyme Activity Report Assigned Genetic Problems from Lecture Quiz #5 on 6 & 10 Nov 5 9. Mendelian Genetics Bring your calculator! 11. Plant Structure Enzyme Activity Report Due Nov 12 Leaf, stem & root drawings Nov 19 Thanksgiving Break—No Labs This Week 12. Microevolution Nov 26 Bring your calculator!
Dec 3 7. Karyotyping Quiz#6 on 9, 11 & 12 8. DNA Extraction and Spooling Karyotype sheet Dec 10 13. Animal Dissection Frog Anatomy Drawing
1 II. Student Evaluation. Your lab and lecture scores will be combined into one grade for the entire course. The following items constitute 25% of the course grade. See your lecture syllabus for more information on grades.
1 lab report 25 Best of 5 drawings and other laboratory work products 25 Best 5 of 6 quizzes at 10 points each 50 possible points for the lab 100
III. Laboratory Drawings. (25 points) A list of materials and guidelines for preparing drawings is given in the introduction to your lab manual. Each drawing set (includes the human karyotype) is worth five points. Of the seven possible sets, the lowest two scores will be dropped. Drawings for missed labs will not be accepted.
IV. Quizzes. (50 points) About every other week a quiz will be administered over the laboratory exercises of the previous two weeks. Each quiz is worth ten points. The lowest score of six quizzes will be dropped. A missed quiz will be counted as your lowest score. There will be no make-up quizzes.
V.Lab Report. (25 points). Two lab exercises will be the subject of lab reports during the term. Half the students will write a report on osmosis from Exercise 4; the other half will write a report on enzyme activity and pH from Exercise 10. More information will be provided in the form of a handout at a later date.
UMPI Writing Center Located on the first floor of South Hall, the writing center offers one on-one consultations for writers at all levels of course work, at all stages of the writing process. Call 768-9615 or stop by to set up an appointment. For more information see: http://www.umpi.edu/programs/cas/english/writing-center.
VI. General Information
Attendance of all laboratory sessions is required. Any student missing more than three laboratory periods will be given an F unless extenuating circumstances can be shown. Missed labs and quizzes cannot be made up. Contact your instructor immediately if you know in advance that you will miss a laboratory period. Do not wait until the next day or later in the week. Lab drawings are due at the end of the laboratory period.
2 General Laboratory Safety Guidelines
I. Emergency Equipment and Telephone Emergency Number: 8-9-1-1
Be prepared to give your name, address (181 Main Street, Presque Isle), Folsom Hall, Room 304 and emergency reason. The dispatcher is able send the appropriate response unit as needed.
II. Location of Safety Equipment in Laboratory
Shower & Rear of lab by window. Eyewash: Fire extinguisher: On the wall on the right side of the dry erase board. First aid kit: On wall near refrigerator. Fire blanket: On left side of laboratory door.
III. General Lab Safety Guidelines
1. Wear appropriate clothing and shoes in the laboratory. If you own a lab coat, bring it to lab. 2. Avoid loose fitting items of clothing. Coats and jackets should be placed on bench designated for this purpose. 3. Food and drinks are not allowed in the laboratory. 4. Keep your workspace free of all unnecessary materials. Backpacks should be place on the floor near your feet, but not in the aisle. 5. Shoulder length hair must be tied back in a pony tail to prevent injury from Bunsen burners and to avoid contamination of laboratory materials. 6. Report spills and accidents immediately to your instructor. 7. Be sure to follow the instructor’s directions in disposal of all chemicals. 8. Only aqueous solutions of inorganic chemicals can be poured into sinks. Organic wastes must be disposed of in appropriate containers marked for this purpose. 9. Donot return unused chemicals to the stock bottles. 10. Dispose of broken glass in yellow receptacle marked for this purpose. 11. Dispose of razor blades and sharp metals objects in red biohazard receptacle. 12. Wash your hands with disinfectant soap provided at the sink before leaving lab.
3 IV. Emergency Procedures for a Laboratory Fire
Do not use a fire extinguisher unless you have been trained. In case of a small fire at your desk, smother with a book or piece of cloth. Do not use your hands. If your clothes are on fire such as a sleeve, run water over it. If the fire has spread beyond a small portion of clothing or appears that it will, use the fire blanket. It is likely that a student will need help with the fire blanket, be prepared to help someone wrap into the fire blanket. If all else fails drop and roll. If a large fire occurs such as in a waste basket, smother the fire with a jacket or other piece of cloth. If the fire is larger, DO NOT ATTEMPT TO PUT OUT THE FIRE. Leave the laboratory. Follow the evacuation plan. If the alarm has not yet sounded, pull the fire alarm on the left side of hall before fire doors leading to the north stairway and continue to the gathering area designated in the evacuation plan.
V. Electrical Equipment
Do not spray or splash water in the vicinity of electrical equipment that is plugged into electrical outlets. If equipment crackles, snaps or begins to smoke, do not attempt to disconnect it, call the instructor immediately. In case of any frayed or broken electrical cords, notify the instructor.
VI. First Aid for Minor Injuries.
Band aids and some basic first aid materials are available in the lab. For spills in the eyes, use the eyewash station. For burns, chill the affected part with cold water and ice as soon as possible.
VII. Evacuation Plan
Should conditions in the laboratory require evacuation due to an accident, hazardous chemical spill, fire or due to fire alarm sounding elsewhere in the building, students will immediately leave the building by the north stairway. Turn left upon exiting the building and proceed to the lawn across from the parking space on the west side of the Folsom Hall. It is important that the class gather together at this location for the instructor to confirm that all students in the course have evacuated the building.
4 Laboratory Etiquette
Lab can run smoother if everyone observes a few simple rules. Individually these items take little effort, but left to your instructor, it adds significantly to laboratory preparation time. Carelessness also affects other courses using the facility so be considerate as you use the lab.
Returning prepared slides to their appropriate boxes. Take one slide at a time, Return it to the same box Place the slide in the box with the label on the left and facing the box front. Put the slides in the box in slots directly opposite. Don’t put the slide in at an angle.
Dispose of your trash in the proper receptacles. Do not leave matches, tissues and pH paper in the trays.
Wipe down all bench space you have used during lab period. If you make a mess at your bench, wipe it up with a sponge or paper towel. Wipe down benches you have used for solutions and equipment with a sponge and soapy water.
Return dissecting tools, test tubes and other equipment to their proper place. If you don’t know where it goes, ask.
Cleaning glassware (slides, tubes, beakers etc.) Remove all tape and label. Wax pencil marks come off readily with a moist paper towel and a bit of elbow grease. Use soapy water to wash the glassware Rinse with tap water and again with distilled water (In wash bottles). Sort beakers and such by type (plastic or glass) and size when returning to their proper shelf or drawer. If you don’t know where they go, please ask.
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