Safety in the Chemistry Laboratory
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AOAC Official Method 2011.25 Insoluble, Soluble, and Total Dietary Fiber in Foods
AOAC Official Method 2011.25 supplies/fisherbrand.html) or 250 mL polypropylene bottles with Insoluble, Soluble, and Total Dietary Fiber in Foods polypropylene caps. Enzymatic-Gravimetric-Liquid Chromatography (c) Fritted crucible.—Gooch, fritted disk, Pyrex® 50 mL, First Action 2011 pore size, coarse, ASTM 40–60 µm (Corning No. 32940-50C® [Applicable to plant material, foods, and food ingredients or equivalent; http://www.labplanet.com/corning-crucible-gooch- consistent with CODEX Alimentarius Commission high-c-50-ml-32940-50c.html). Prepare four for each sample as Definition adopted in 2009 and modified slightly in 2010 follows: Ash overnight at 525°C in muffle furnace. Cool furnace to (ALINORM 09/32/REP and ALINORM 10/33/REP, respectively), 130°C before removing crucibles to minimize breakage. Remove including naturally occurring, isolated, modified, and synthetic any residual Celite and ash material by using a vacuum. Soak in 2% polymers meeting that definition.] cleaning solution, C(i), at room temperature for 1 h. Rinse crucibles See Tables 2011.25A–H for the results of the interlaboratory with water and deionized water. For final rinse, use 15 mL acetone study supporting acceptance of the method. and air dry. Add approximately 1.0 g Celite to dried crucibles and A. Principle dry at 130°C to constant weight. Cool crucible in desiccators for approximately 1 h and record mass of crucible containing Celite. A method is described for the measurement of insoluble, (d) Filtering flask.—Heavy-walled, 1 L with side arm. soluble, and total dietary fiber (IDF, SDF, and TDF, respectively), (e) Rubber ring adaptors.—For use to join crucibles with inclusive of the resistant starch (RS) and the water:alcohol soluble filtering flasks. -
VACUUM RECOVERY of ASPHALT EMULSION RESIDUE (An Arizona Method)
ARIZ 504 July 1980 (3 Pages) VACUUM RECOVERY OF ASPHALT EMULSION RESIDUE (An Arizona Method) Scope (d) No. 10 sieve conforming to AASHTO designation M 92. I. This method describes a low temperature vacuum procedure for recovery of the asphalt residue (e) Vacuum recovery apparatus as shown from asphalt emulsions. It is is not suitable for assembled in Fig. I. quantitative recovery of solvents from emulsions I) Vacuum source capable of producing an containing low boiling range distillates. absolute vacuum within the system of approximately 710 mm (28 in.) mercury. Apparatus 2) Thermometer - shall have a range of _5° to 1. The apparatus shall consist of the following: +200°C (23°F to 392°F). The overall length (a) Brass stirring rod. shall be 600 mm (24 in.) and the distance from the bottom of the bulb to the zero point (b) 8 oz. ointment can. shall be 300 mm (12 in.) (c) 100 ml. stainless steel beaker. 3) Stirrer hot plate. VACUUM RECOVERY APPARATUS 300 mm Allihn condenser H20 Out / Thermometer Vacuum Release Pinch Clamp 500 m. 1,000 ml. Filtration Flask Filtration Flask Teflon Stirring Bar H20 In 500 ml. Filtration Flask Portable Heat Gun FIGURE I ARIZ 504 July 1980 4) Teflon covered stirring bar. (g) Insert the stoppered themometer (positioned 5) 1000 ml. and two 500 ml. filtering flasks with in the stopper at an angle to prevent contact with tubulation. stirring bar) into the flask and set on hot plate at a medium high heat setting (#4). The bulb of the 6) 300 mm Allihn condensor. -
Zinc and Cadmium in Paper (Reaffirmation of T 438 Cm-96)
WI 050114.01 T 438 DRAFT NO. 5 DATE July 27, 2006 TAPPI WORKING GROUP CHAIRMAN J Ishley SUBJECT CATEGORY Fillers & Pigments Testing RELATED METHODS See “Additional Information” CAUTION: This Test Method may include safety precautions which are believed to be appropriate at the time of publication of the method. The intent of these is to alert the user of the method to safety issues related to such use. The user is responsible for determining that the safety precautions are complete and are appropriate to their use of the method, and for ensuring that suitable safety practices have not changed since publication of the method. This method may require the use, disposal, or both, of chemicals which may present serious health hazards to humans. Procedures for the handling of such substances are set forth on Material Safety Data Sheets which must be developed by all manufacturers and importers of potentially hazardous chemicals and maintained by all distributors of potentially hazardous chemicals. Prior to the use of this method, the user must determine whether any of the chemicals to be used or disposed of are potentially hazardous and, if so, must follow strictly the procedures specified by both the manufacturer, as well as local, state, and federal authorities for safe use and disposal of these chemicals. Zinc and cadmium in paper (Reaffirmation of T 438 cm-96) (no changes were made since last draft) 1. Scope and significance 1.1 This method maybe used for the determination of cadmium and zinc either in paper or in highly opaque pigments. Zinc is usually present in zinc oxide, zinc sulfide, or as lithopone (a combination of zinc sulfide and barium sulfate), which is occasionally used in filled paper, in paper coatings and in high-pressure laminates and wallpaper. -
Detection of Acid-Producing Bacteria Nachweis Von Säureproduzierenden Bakterien Détection De Bactéries Produisant Des Acides
(19) TZZ ¥ _T (11) EP 2 443 249 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12Q 1/04 (2006.01) G01N 33/84 (2006.01) 19.11.2014 Bulletin 2014/47 (86) International application number: (21) Application number: 10790013.6 PCT/US2010/038569 (22) Date of filing: 15.06.2010 (87) International publication number: WO 2010/147918 (23.12.2010 Gazette 2010/51) (54) DETECTION OF ACID-PRODUCING BACTERIA NACHWEIS VON SÄUREPRODUZIERENDEN BAKTERIEN DÉTECTION DE BACTÉRIES PRODUISANT DES ACIDES (84) Designated Contracting States: (74) Representative: Isarpatent AL AT BE BG CH CY CZ DE DK EE ES FI FR GB Patent- und Rechtsanwälte GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Friedrichstrasse 31 PL PT RO SE SI SK SM TR 80801 München (DE) (30) Priority: 15.06.2009 US 187107 P (56) References cited: 15.03.2010 US 314140 P US-A- 4 528 269 US-A- 5 098 832 US-A- 5 164 301 US-A- 5 601 998 (43) Date of publication of application: US-A- 5 601 998 US-A- 5 786 167 25.04.2012 Bulletin 2012/17 US-B2- 6 756 225 US-B2- 7 150 977 (73) Proprietor: 3M Innovative Properties Company • DARUKARADHYA J ET AL: "Selective Saint Paul, MN 55133-3427 (US) enumeration of Lactobacillus acidophilus, Bifidobacterium spp., starter lactic acid bacteria (72) Inventors: and non-starter lactic acid bacteria from Cheddar • YOUNG, Robert, F. cheese", INTERNATIONAL DAIRY JOURNAL, Saint Paul, Minnesota 55133-3427 (US) ELSEVIER APPLIED SCIENCE, BARKING, GB, • MACH, Patrick, A. -
15.0 Hazardous Waste Disposal
LABORATORY SAFETY MANUAL Revised June 2018 Emergency Contact Information In case of emergency: 911 Security Services: (daytime) 705-474-7600 ext. 5555 (cell phone) 705-498-7244 Laboratory Safety Coordinator: 474-3450 ext. 4180 Emergency Procedures** Chemical Spill On Body • Rinse affected area immediately for 15 minutes using emergency shower, if required. • Care must be taken to avoid contamination with face and eye(s). In Eye(s) • Immediately flush eye(s) using emergency eyewash station for a minimum of 15 minutes. In Laboratory • Assess the scene. If the situation is beyond your capabilities, contact your supervisor. • If safe to do so, turn off any ignition sources if flammable material is present. • Use spill kits to assist in spill containment. • Dispose of waste material with other hazardous waste. Fire • If you hear the Fire Alarm ring continuously evacuate the building immediately. • If you hear the Fire Alarm ring intermittently be alert however evacuation is not necessary. • If Fire Alarm changes from intermittent to continuous evacuate immediately • If you detect a fire, do not attempt to extinguish it yourself unless you are capable and it is safe to do so. Report the injury/incident to your supervisor as soon as possible. ** This is to be used as a quick reference only. For more detailed information, please refer to section 8.0 Emergency Procedures. Laboratory Safety Manual – Nipissing University 2 Table of Contents Acknowledgements ................................................................................................... -
Performance Validation of the Microbiologique Microfilm Test
MAI ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 101, NO. X, 2018 1 FOOD BIOLOGICAL CONTAMINANTS Performance Validation of the Microbiologique MicrofilmTM Test System for AOAC Research Institute Performance Tested Method SMCertification AOAC Performance Tested Method SM 051702 Abstract ANNA SHAPOVALOVA and HARISH K. JANAGAMA Molecular Epidemiology, Inc., 15300 Bothell Way NE, The Microfilm™ Test System is intended for quantitative Lake Forest Park, WA 98155 microbiology and consists of three types of Microfilms for aerobic LONG VUONG and ALEX FRIEDRICH plate count (Microfilm APC), total coliform and Escherichia coli IEH Laboratories and Consulting Group, Inc., 15300 Bothell count (Microfilm TCEc), and yeast and mold count (Microfilm Way NE, Lake Forest Park, WA 98155 YMC). This study evaluated the performance of the Microfilm DYLAN JOHNSON Test System against International Organization for Standardization Molecular Epidemiology, Inc., 15300 Bothell Way NE, (ISO) methods on 20 food matrixes and 2 environmental surfaces. Lake Forest Park, WA 98155 Ruggedness, robustness, and stability were also determined, LOURDES M. NADALA while inclusivity and exclusivity studies were performed on Molecular Epidemiology, Inc., 15300 Bothell Way N.E., Microfilm TCEc and YMC. An independent laboratory evaluated Lake Forest Park, WA 98155; Microbiologique, the performance on four food matrixes and one environmental Inc., 8215 Lake City Way NE, Seattle, WA 98115 surface. No significant differences and high correlation coefficients VAN NGUYEN were observed between the Microfilm Test System and the Microbiologique, Inc., 8215 Lake City Way NE, Seattle, corresponding ISO methods (ISO 4833-1:2013 for APC, ISO WA 98115 4832:2006 for total coliform count, ISO 16649-2: 2001 for E. -
Safety in the Chemistry Lab
Safety in the Chemistry Lab Working in the chemistry laboratory is an inter- 12. Never touch any substance in the lab un- esting and rewarding experience. During your less specifically instructed to do so by your labs, you will be actively involved from beginning teacher. to end—from setting some change in motion to 13. Never put your face near the mouth of a drawing some conclusion. In the laboratory, you container that is holding chemicals. will be working with equipment and materials that 14. Never smell any chemicals unless in- can cause injury if they are not handled properly. structed to do so by your teacher. When testing for However, the laboratory is a safe place to work if odors, use a wafting motion to direct the odors to you are careful. Accidents do not just happen, they your nose. are caused—by carelessness, haste, and disregard 15. Any activity involving poisonous vapors of safety rules and practices. Safety rules to be should be conducted in the fume hood. followed in the laboratory are listed below. Before 16. Dispose of waste materials as instructed beginning any lab work, read these rules, learn by your teacher. them, and follow them carefully. 17. Clean up all spills immediately. 18. Clean and wipe dry all work surfaces at General the end of class. Wash your hands thoroughly. 19. Know the location of emergency equip- 1. Be prepared to work when you arrive at ment (first aid kit, fire extinguisher, fire shower, the laboratory. Familiarize yourself with the lab fire blanket, etc.) and how to use them. -
Gas Generator Bottle Introduction SCIENTIFIC This Gas Generator Setup Provides an Easy Way to Generate and Collect Gas
Gas Generator Bottle Introduction SCIENTIFIC This gas generator setup provides an easy way to generate and collect gas. Specific instructions are provided for the generation of hydrogen gas using zinc and acid. Concepts • Generation of gases • Water displacement Materials Hydrochloric acid solution, HCl, 3 M Glass plates or Sulfuric acid solution, H2SO4, 3 M Glass tubing Mossy zinc, Zn, 6 g Pneumatic trough Water, tap Rubber tubing Bent glass tubing* Silicone grease packet* Gas collecting bottles or tubes, 3 or 4 Thistle tube* Gas generator bottle* Two-hole rubber stopper* *Materials included. Safety Precautions Hydrochloric acid solution is toxic by ingestion and inhalation and is severely corrosive to skin, eyes and other tissues, as is sulfuric acid solu- tion. Hydrogen gas is a highly flammable gas and a severe fire hazard. Exercise extreme caution when testing the gas and keep the gas generator away from flames. Wear chemical splash goggles, chemical-resistant gloves, and a chemical-resistant apron. This activity requires the use of hazardous components and/or has the potential for hazardous reactions. Please review current Material Safety Data Sheets for additional safety, handling, and disposal information. Procedure 1. Set up the apparatus as shown in the figure to the right. Lubricate the glass tubing and thistle tube with silicone grease before inserting into the stopper. Make sure Thistle tube the water level is above the platform. Prepare bottles for collecting gas by water Two-hole rubber stopper displacement. To do this, fill each gas collecting bottle (or tube) over the brim with tap water, and then cover each with a flat glass plate. -
METHODS & STANDARD OPERATING PROCEDURES (Sops)
METHODS & STANDARD OPERATING PROCEDURES (SOPs) OF EMISSION TESTING IN HAZARDOUS WASTE INCINERATOR FOREWARD COVR PAGE TEAM CHAPTER TITLE PAGE NO Chapter - 1 Stack Monitoring – Material And Methodology For Isokinetic Sampling 1-28 Chapter - 2 Standard Operating Procedure (SOP) For Particulate Matter Determination 29-38 Chapter - 3 Determination Of Hydrogen Halides (Hx) And Halogens From Source Emission 39-54 Chapter - 4 Standard Operating Procedure For The Sampling Of Hydrogen Halides And Halogens From Source Emission 55-61 Determination Of Metals And Non Metals Emissions From Chapter - 5 Stationarysources 62-79 Standard Operating Procedure (Sop) For Sampling Of Metals And Non Chapter - 6 Metals & 80-97 Standard Operating Procedure (Sop) Of Sample Preparation For Analysis Of Metals And Non Metals Chapter - 7 Determination Of Polychlorinated Dibenzo-P-Dioxins And 98-127 Polychlorinated Dibenzofurans Standard Operating Procedure For Sampling Of Polychlorinated Chapter - 8 Dibenzo-P-Dioxins And Polychlorinated Dibenzofurans & 128-156 Standard Operating Procedure For Analysis Of Polychlorinated Dibenzo- P-Dioxins And Polychlorinated Dibenzofurans Project Team 1. Dr. B. Sengupta, Member Secretary - Overall supervision 2. Dr. S.D. Makhijani, Director - Report Editing 3. Sh. N.K. Verma, Ex Additional Director - Project co-ordination 4. Sh. P.M. Ansari, Additional Director - Report Finalisation 5. Dr. D.D. Basu, Senior Scientist - Guidance and supervision 6. Sh. Paritosh Kumar, Sr. Env. Engineer - Report processing 7. Sh. Dinabandhu Gouda, Env. Engineer - Data compilation, analysis and preparation of final report 8. Sh. G. Thirumurthy, AEE - - do - 9. Sh. Abhijit Pathak, SSA - - do - 10. Ms. Trapti Dubey, Jr. Professional (Scientist) - - do - 11. Er. Purushotham, Jr. Professional (Engineer) - - do - 12. -
ARC Laboratory Manual for Architectural Conservators
A LABORATORY MANUAL FOR ARCHITECTURAL CONSERVATORS Jeanne Marie Teutonico ICCROM Rome 1988 CONTENTS Foreword v Preface vii General Principles: Laboratory Science 1. Sampling 3 2. Measurement and Error: Precision, Accuracy, Statistics 7 3. Measurement: Mass (Use of the Balance) 11 4. Measurement: Length (Use of the Vernier Caliper and Micrometer) 16 5. Measurement: Volume 21 6. Measurement: Solutions 26 7. Measurement: pH 30 Porous Building Materials 8. Water Absorption by Total Immersion 35 9. Water Drop Absorption 41 10. Penetration of Water: Capillary Action 43 11. Porosity of Granular Beds 45 12. Porosity in Solids: Indirect Measurement by Water Absorption 50 13. Porosity in Solids: Hydrostatic Weighing 52 14. Movement of Salts 56 15. Salt Crystallization 57 16. Qualitative Analysis of Water-soluble Salts and This publication was printed with a generous Carbonates 58 contribution from the government of 17. Semiquantititive Analysis of Water-soluble Salts 68 Finland. Earthen Building Materials 18A. Particle Size Analysis: Part I Sieving Procedure 73 ISBN 92-9077-083-X Via di San Michele 13 00153 Rome RM, Italy Printed in Italy III Earthen Building Materials (continued) FOREWORD 18B. Particle Size Analysis: Part II Sedimentation Procedure: Hydrometer Method 83 19. Plastic Limit of Soils 96 After his appointment as director of ICCROM in 1977, Sir Bernard FEILDEN proposed to emphasize the interdisciplinary 20. Liquid Limit of Soils 102 character of conservation by introducing scientists to field work and architects to laboratories. Consequently it was decided to introduce a series of laboratory exercises Stone. Brick and Mortars designed for the needs of architectural conservators and conservation architects in the program of the International 21. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Preparing Sodium Hydroxide Solutions Nov 2016
STANDARD OPERATING PROCEDURE: Preparing sodium hydroxide solutions Note: To be undertaken only by trained personnel in conjunction with a current Safety Data Sheet (SDS) and site-specific risk assessment. ___________________ 1. Introduction Sodium hydroxide (NaOH) is a hazardous chemical and must be treated with care. It is highly corrosive to skin and eyes and extremely toxic if ingested. The solid can be purchased as pellets, flakes or mini pearls. It is very hygroscopic (absorbs water from the air) and will also absorb carbon dioxide from the air and therefore cannot be used as a primary standard for titrations. To minimise its absorption of water and carbon dioxide, it should be kept in a tightly closed container and left open for as little time as possible. Dissolving sodium hydroxide in water is an exothermic (heat producing) reaction. Synonyms: caustic soda, lye, soda lye. 2. Context These instructions are for the use of experienced teachers and technicians only. Do not make up a solution for the first time without seeking practical advice from an experienced colleague. 3. Safety Notes This activity may only be carried out with appropriate facilities available i.e. running water, chemical safety/eyewash station and relevant Personal Protective Equipment (PPE) Conduct procedure in a well-ventilated area or fume cupboard. Avoid contact with skin and eyes, and avoid breathing fumes. The preparation of sodium hydroxide solutions liberates heat and may produce caustic fumes/vapours. Ensure that glassware is free from chips and cracks before use. For first aid, accident and spill procedures refer to SDS before making a solution.