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PRACTICAL Safety SCIENCE 101 Frugal Science - Foldscope General Safety ★ LISTEN to your instructors. ★ Always FOLLOW the directions provided for the lab. ★ Do not EAT or DRINK anything in the lab. ★ Please be OFF your CELLPHONES during lectures or experiments. It is disrespectful and disturbing to others. You may be allowed to use your phones only for taking pictures or videos. General Safety

★ Tie back LOOSE HAIR.

★ Roll up LOOSE SLEEVES.

★ Please keep your HANDS AWAY from your face. It may cause serious irritations or pain if chemicals get in your eye. General Safety ★ Never SMELL, TASTE, or TOUCH any chemicals in the lab.

★ If an accident or a spill occurs, please NOTIFY THE TEACHERS.

★ After working with chemicals, alwaysWASH YOUR HANDS

with soap & water.

★ PROPERLY DISPOSE chemicals as instructed to do so. Personal Protective Equipment (PPE) ★ Wear SAFETY GOGGLES at all times during an experiment. ★ GLOVES should be worn to protect your hands from touching chemicals when appropriate. ★ Always wear closed-toe shoes in the lab. No sandals or slippers in the lab! ★ Aprons/lab coats will be given if necessary. Precautions ★ Know the LOCATION of the fire extinguisher, fire blanket, station, and safety shower. ★ Use to extinguish fires on PEOPLE ★ Although it is unlikely, if a fire occurs in the , you should FOLLOW THE DIRECTIONS of your instructors. Remember the acronyms RACE and PASS. Do not panic. FIX THE LAB #1 FIX THE LAB #2 Intro to Microscopy

● A is an instrument used to see objects that are too small to be seen by the naked eye. ● Microscopy is the science of investigating small objects and structures using such a microscope. ● The first simple compound microscope was made by Robert Hooke in the 1600s. Parts of a Microscope 1. Eyepiece/Ocular lens 2. Diopter adjustment 3. Nosepiece 4. Arm 5. Objective lens 6. Slide holder 7. Stage 10 8. 9. Iris Diaphragm 12 10. Coarse focus knob 11. Fine focus knob 12. Light source 13. Base 14. On/Off Switch Foldscope and Frugal Science

Frugal science: The development of ultra low-cost tools for the improvement and accessibility of science in underdeveloped and developing regions ● Ex. 3D Fuge, Electropen, 3D printed Foldscope: an “ultra-affordable, paper microscope” that is considered to be the cheapest microscope in the world. It was developed by Manu Prakash at Stanford University. It is capable of magnifying 140x. Credits: Prakash Lab at Stanford

Examples

Onion Cells Algae Mosquito

Credit: Microcosmos Community Procedure and Materials Materials ● Plastic or dropper ● Sterile, individually packed cotton swabs ● Glass microscope slides ● Plastic coverslips ● Paper towels or tissue Protocol: 1. Pipette one drop of pond water onto microscope slide. 2. Place clear plastic cover over sample on microscope slide. 3. Remove any excess solution by allowing a paper towel to touch one side of the coverslip. 4. Place the slide in the Foldscope, with 4 x or 10 x objective in position and find a cell. Then view your slide at higher magnification. JEOPARDY - LET’S REVIEW

https://docs.google.com/presentation/d/1ViM_jcfKhzQitAV0xuX4wvi4dTfyP0lcPMivuhe_E3E/edit ?usp=sharing TODAY WE LEARNED...

WHAT SAFETY WHAT IS THE PROCEDURES SHOULD ONE FOLDSCOPE + HOW WHAT IS TAKE IN THE LAB? DOES IT WORK? MICROSCOPY?

Foldscope: an A microscope is an “ultra-affordable, paper microscope” that is Always listen to instrument used to considered to be the see objects that are cheapest microscope in the your instructor world. It was developed by too small to be seen Manu Prakash at Stanford by the naked eye. University. It is capable of magnifying 140x. DoesTHANK anyone have any finalYOU! questions? THE END.

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