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Home , Ozagrel, Prostaglandin G2, Prostaglandin H2, Seratrodast, Thromboxane A2

Pathway

Diverse physical, chemical, Phospholipase A inflammatory, and 2 mitogenic stimuli

NSAIDs NSAIDs

Prostaglandin G2 CYCLOOXYGENASE G2

Prostaglandin H Prostaglandin H Synthase-1 Synthase-2 (COX 1) (COX 2)

Prostaglandin H2 PEROXIDASE

Tissue Specific Isomerases

Prostacyclin A2 Prostaglandin F2α

IP TPα, TPβ DP1, DP2 EP1, EP2, EP3, EP4 FPα, FPβ

Endothelium, Kidney, , Vascular Mast Cells, Brain, Brain, Kidney, Vascular Uterus, Airways, Vascular Platelets, Brain Smooth Muscle Cells, Airways, Lymphocytes, Smooth Muscle Cells, Smooth Muscle Cells, Macrophages, Kidney Eosinophils Platelets Eyes Item No. Product Features

Prostacyclin (Prostaglandin I2; PGI2) is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential 515211 6-keto • Sample Types: Culture Medium | Plasma Prostaglandin • Measure 6-keto PGF levels down to 6 pg/ml activities of COX and . PGI2 is non-enzymatically 1α F ELISA Kit • Incubation: 18 hours | Development: 90-120 minutes | hydrated to 6-keto PGF1α (t½ = 2-3 minutes), and then quickly converted 1α Read: Colorimetric at 405-420 nm to the major metabolite, 2,3-dinor-6-keto PGF1α (t½= 30 minutes). Prostacyclin was once thought to be a circulating hormone that regulated • Assay 24 samples in triplicate or 36 samples in duplicate

-vasculature interactions, but the rate of secretion into circulation • NOTE: A portion of urinary 6-keto PGF1α is of renal origin coupled with the short half-life indicate that prostacyclin functions • NOTE: It has been found that normal plasma levels of 6-keto PGF may be low locally. Estimates of systemic PGI2 production have often been assessed 1α by measurement of 6-keto PGF1α alone or in combination with 2,3-dinor- 501100 Prostaglandin I • Sample Type: Urine 6-keto PGF1α. However, the majority of 6-keto PGF1α in urine is of renal Metabolite • Measure PGIM down to 120 pg/ml origin with only 14% originating from plasma. ELISA Kit • Convenient overnight format COOH • NOTE: A minimum of a 1:10 dilution is recommended Arachidonic Acid when assaying urine samples Sample types not noted may still be assayed but have not been validated by Cayman Scientists COX

O COOH PGH Prostacyclin Pathway Reagents 2 O Synthase OH Antibodies/Probes Agonists Antagonists Inhibitor Prostacyclin Synthase IP (human) Prostaglandin I2 CAY10441 U-51605 Polyclonal Antibody (sodium salt) 10005186 16465 10005518 18220 COO- Na+ AFP 07 (free acid) O IP Receptor (mouse) CAY10449 Polyclonal Antibody 13626 10005913

PGI2 160070 (sodium salt)

HO 18230 OH Prostaglandin I Synthase Polyclonal Antibody Non-enzymatic 160640 18215 OH NS-304 COOH Prostaglandin I Synthase 6-keto PGF1α 10010411 O Monoclonal HO Antibody (Clone isn-1) Carbaprostacyclin OH 160630 18210 -oxidation Cicaprost β Prostaglandin I Synthase 16831 OH Monoclonal Antibody COO- Na+ Ciprostene (calcium salt) 2,3-dinor-6-keto PGF1α (Clone 3C8) O 10247 18216 HO OH Prostaglandin I Synthase 10162 (mouse) Polyclonal Antibody MRE-269 100023 10010412 Taprostene (free acid) Anti-PGI 10011348 Synthase:SureLight® R-PE 16690

Thromboxane A2 O COOH

O Thromboxane A (TXA ) is produced from arachidonic acid by many cells and 2 2 PGH OH causes irreversible platelet aggregation and vascular and bronchial smooth 2 Thromboxane Synthase muscle contraction. TXA2, like most lipid mediators, is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a

short distance of its biosynthesis. TXA is rapidly hydrolyzed non-enzymatically COOH 2 O

to form (TXB2). Although it is common to estimate TXA2 levels TXA OH by measuring TXB2, most of the TXB2 measured is due to ex vivo platelet 2 activation or intra-renal production. Measurement errors are compounded Non-enzymatic by the fact that normal concentrations of circulating TXB are extremely low 2 OH (1-2 pg/ml), and highly transient (t = 5-7 minutes). To circumvent this problem, ½ COOH it is necessary to measure a metabolite that cannot be formed by platelets or HO O by the kidney. TXB2 may be metabolized by 11-hydroxy TX dehydrogenase OH TXB2 to form 11-dehydro TXB2, or by β-oxidation to form 2,3-dinor TXB2. Infusion 11-hydroxy TX dehydrogenase β-oxidation studies using TXB2 have shown that both metabolites are formed equally, although 11-dehydro TXB2 has a longer circulating half-life (t½ = 45 minutes). OH Therefore, measurement of 11-dehydro TXB2 in plasma or urine will give a OH COOH COOH time-integrated indication of TXA2 production. The circulating half-life of O 2,3-dinor TXB2 is shorter (t = 15 minutes). Therefore, measurement of O HO O ½ OH 2,3-dinor TXB will give a more episodic indication of TXA production. OH 2 2 11-dehydro TXB2 2,3-dinor TXB2

2 Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • www.caymanchem.com Thromboxane Pathway Reagents Antibodies/Probes Agonists Antagonists Synthase Inhibitors

TP Receptor (human) Polyclonal FITC Antibody 10012559 Carbocyclic 19010 I-SAP 19021 Furegrelate (sodium salt) 70540 TP Receptor (human) Polyclonal Antibody 10004452 U-46619 16450 BAY-u3405 10156 70515 TP Receptor (mouse) Polyclonal Antibody 101882 I-BOP 19600 SQ 29,548 19025 1-Benzylimidazole 70510

Thromboxane B2 11-dehydrogenase Polyclonal Antiserum 160720 U-44069 16440 9002014 BM 567 10155 Thromboxane Synthase Polyclonal Antibody 160715 S18886 14059 GW 627368X 10009162 BM 567 10155 CAY10535 10010396 AH 23848 (calcium salt) 19023 L-655,240 10011562

Thromboxane B2

A portion of TXB2 measured in urine is of renal origin and does not represent systemic TXB2 formation, thus selecting an appropriate assay for urinary

TXB2 is dependent on the application. The TX metabolite 11-dehydro TXB2 is better suited for measurement of systemically produced TXB2 in urine samples.

Item No. Product Features

501020 Thromboxane B2 ELISA Kit • Sample Types: Culture Medium | Serum | Urine

• Measure TXB2 levels down to 5 pg/ml • Incubation : 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

10004023 Thromboxane B2 Express ELISA Kit - Monoclonal • Sample Types: Culture Medium | Serum | Plasma

• Measure TXB2 levels down to 45 pg/ml • Rapid assay; get results in under 4 hours • Assay 24 samples in triplicate or 36 samples in duplicate

519510 11-dehydro Thromboxane B2 ELISA Kit - Monoclonal • Sample Type: Urine • Assay 24 samples in triplicate or 36 samples in duplicate • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

• Detect 11-dehydro TXB2, a stable urinary metabolite of TXA2 and TXB2 Sample types not noted may still be assayed but have not been validated by Cayman Scientists

Prostaglandin D2

Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione- O COOH O independent lipocalin-type PGD2 synthase.

This PGD2 accumulates in the cerebrospinal PGH2 OH fluid (CSF), where it induces physiologic

sleep in rats and humans. PGD2 is also PGD Synthase synthesized in mast cells and leukocytes

by a cellular, myeloid-type, glutathione- OH dependent PGD synthase. This PGD2, which is formed in intracellular and vascular COOH compartments, is rapidly metabolized to O OH 11β-PGF2α. Thus, urinary measurements PGD2 of PGD2 synthesis are most appropriately focused on the measurement of 11β-PGF2α. Measurement of the parent PGD2 is appropriate in the supernatants OH of cell cultures, where PGD2 levels may reach several ng/ml, and in CSF, where COOH concentrations of several hundred pg/ml HO have been measured. OH 11β-PGF2α

For full information on these products and more, visit www.caymanchem.com 3 Item No. Product Features

512031 Prostaglandin D2 ELISA Kit • Sample Types: Cell Lysates | Culture Medium

• Measure PGD2 levels down to 55 pg/ml • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

512041 Prostaglandin D2 Express ELISA Kit • Sample Types: Cell Lysates | Culture Medium

• Measure PGD2 levels down to 350 pg/ml • Rapid assay; get results in under 4 hours • Assay 24 samples in triplicate or 36 samples in duplicate

512011 Prostaglandin D2-MOX ELISA Kit • Sample Types: Culture Medium | Plasma

• Measure PGD2-MOX levels down to 3.1 pg/ml • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm

• Treatment of samples with MOX hydrochloride converts unstable PGD2 to stable PGD2-MOX • Highly sensitive • NOTE: Derivatization of standards and samples is required before running assay

500151 Prostaglandin D2-MOX Express ELISA Kit • Sample Types: Culture Medium | Plasma

• Measure PGD2-MOX levels down to 16 pg/ml • Rapid assay; get results in under 4 hours • Assay 24 samples in triplicate or 36 samples in duplicate

• Treatment of samples with MOX hydrochloride converts unstable PGD2 to stable PGD2-MOX; MOX derivatization reagent included with assay

516521 11β-Prostaglandin F2α ELISA Kit • Sample Types: Plasma | Urine

• Measure 11β-PGF2α levels down to 5 pg/ml • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

• NOTE: 11β-PGF2α may not be present in mouse urine – see Item No. 501001 501001 tetranor-PGDM ELISA Kit • Sample Type: Urine • Measure tetranor-PGDM levels down to 40 pg/ml • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate Sample types not noted may still be assayed but have not been validated by Cayman Scientists

DP Receptor Reagents PGD Synthase Antibodies Agonists Antagonists 2 PGD Synthase Antibodies Inhibitors

DP1 DP1 Receptor Prostaglandin D2 12010 AH 6809 14050 AT-56 13160 Polyclonal BW 245C 12050 MK-0524 10009835 HQL-79 10134 Antibody 101640 Prostaglandin J2 18500 BW A868C 12060 BW 246C 12055

DP2 CRTH2/DP2 Prostaglandin D2 12010 BAY-u3405 10156 Prostaglandin D Synthase (CRTH2) Receptor (C-Term) (hematopoietic-type; human) Monoclonal Polyclonal 15(R)-15-methyl Prostaglandin D2 12720 CAY10471 10006735 Antibody (Clone 2A5) 10004345 Antibody 10007002 13,14-dihydro-15-keto Prostaglandin D2 12610 CAY10595 10012553 Prostaglandin D Synthase (hematopoietic-type; mouse) Monoclonal CRTH2/DP 2 Prostaglandin J 18500 Antibody (Clone 7H4) 10004349 Receptor 2 CAY10597 10012539

(N-Term) 15-deoxy-Δ12,14-Prostaglandin J 18570 2 OC000459 12027 Prostaglandin D Synthase Polyclonal (hematopoietic-type) Polyclonal Antibody Antibody 10004886 12,14 15-deoxy-Δ -Prostaglandin J2-2-glycerol ester 10010132 160013

12 Δ -Prostaglandin J2 18550 Prostaglandin D Synthase (hematopoietic-type; human) Polyclonal 17-phenyl trinor Prostaglandin D2 12810 Antibody 10004337

12,14 15-deoxy-Δ -Prostaglandin D2 12700 Prostaglandin D Synthase (hematopoietic-type; mouse) Polyclonal Indomethacin 70270 Antiserum 10004348

11-keto Fluprostenol 16783 Prostaglandin D Synthase (lipocalin-type; human) Monoclonal Antibody (Clone Prostaglandin D 12990 3 10A5) 10004342

15(R)-Prostaglandin D 10118 2 Prostaglandin D Synthase (hematopoietic- type) Polyclonal Antibody 160003 15(S)-15-methyl Prostaglandin D 12730 2 Prostaglandin D Synthase (lipocalin-type; 16,16-dimethyl Prostaglandin D 12750 2 mouse) Polyclonal Antibody 10004344

4 Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • www.caymanchem.com Prostaglandin E2 O COOH

O

OH Prostaglandin E2 (PGE2) is one of the primary arachidonic acid metabolites via PGH2 the COX pathway and one of the most widely investigated (PGs). Like most , it does not exist preformed in any cellular reservoir. PGE Synthase

When cells are activated or exogenous free arachidonate is supplied, PGE2 is O synthesized de novo and released into the extracellular space. In vivo, PGE2 is COOH

rapidly converted to an inactive metabolite (13,14-dihydro-15-keto PGE2) by the HO OH PG 15-dehydrogenase pathway. The half-life of PGE2 in the circulatory system is PGE2 approximately 30 seconds and normal plasma levels are 3-12 pg/ml. Because of 15-OH PGDH the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is O often best accomplished by the measurement of PGE2 metabolites. PGE2 activity influences inflammation, fertility and parturition, gastric mucosal integrity, and COOH

immune modulation. Because of its involvement in inflammation, PGE is a key HO 2 O indicator of NSAID efficacyvia inhibition of COX-1 and COX-2. 15 keto PGE2 15-oxoprostaglandin Δ13-reductase

O

COOH

HO O

13,14-dihydro-15-keto-PGE2

Non-enzymatic degradation

O

COOH

O

13,14-dihydro-15-keto-PGA2

PGE2 Pathway Reagents PGE Syn- Receptor Synthase 2 Agonists Antagonists thase Antibodies Antibodies Inhibitors EP Receptor Polyclonal Antibody 101740 14010 AH 6809 14050 -1 (microsomal) CAY10526 EP1 1 2 17-phenyl trinor Prostaglandin E2 14810 SC-19220 14060 Polyclonal Antibody 160140 10010088 ONO-8711 14070 SC-51089 10011561 Prostaglandin E Synthase-1 (microsomal) YS-121 SC-51322 10010744 Monoclonal Antibody (Clone 6C6) 10004350 13665 GW 848687X 10010410 Prostaglandin E CAY10589 Synthase-2 (microsomal) 13164 EP Receptor Polyclonal Antibody 101750 Prostaglandin E 14010 AH 6809 14050 EP2 2 2 Polyclonal EP Receptor Polyclonal PE Antibody 10477 Butaprost 13740 PF-04418948 15016 2 Antibody 160145 CAY10678 Anti-EP Receptor:SureLight® R-PE 16684 11-deoxy-16,16-dimethyl Prostaglandin E 14570 TG4-155 17639 2 2 15129 19(R)-hydroxy Prostaglandin E 14910 2 Prostaglandin E Synthase 13820 (cytosolic) Polyclonal MF63 Antibody 160150 13217

Prostaglandin E Synthase (cytosolic) CAY10686 EP Receptor Polyclonal Antibody 101760 Prostaglandin E 14010 AH 6809 14050 EP3 3 2 Monoclonal Antibody 9001962 14765 L-798,106 11129 (Clone JJ6) 18219

17-phenyl trinor Prostaglandin E2 14810 Pirinixic Acid

11-deoxy-16,16-dimethyl Prostaglandin E2 14570 Prostaglandin E Synthase (cytosolic, FL) Aminothiazole Misoprostol 13820 Polyclonal Antibody 10209 15483

EP Receptor (N-Term) Polyclonal Antiserum 101770 Prostaglandin E 14010 GW 627368X 10009162 EP4 4 2 EP4 Receptor (C-Term) Polyclonal Antibody 101775 CAY10580 16835 L-161,982 10011565

EP4 Receptor (C-Term) Polyclonal PE Antibody 10479 L-902,688 10007712 AH 23848 (calcium salt) 19023 ® Anti-EP4 Receptor (C-Term):SureLight APC 16625 Misoprostol 13820 MF498 15973 KMN-80 15435 CJ-42794 10010428 Rivenprost 13618 CJ-023423 10010355 CAY10684 15966 ON-AE3-208 14522

For full information on these products and more, visit www.caymanchem.com 5 Item No. Product Features

514010 Prostaglandin E2 ELISA Kit - Monoclonal • Measure PGE2 in multiple sample types

• Measure PGE2 levels down to 15 pg/ml • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

• NOTE: PGE2 is found in urine, but it is of mixed systemic and renal origin

500141 Prostaglandin E2 Express ELISA Kit • Measure PGE2 in multiple sample types

• Measure PGE2 levels down to 36 pg/ml • Rapid assay; get results in under 4 hours • Assay 24 samples in triplicate or 36 samples in duplicate

• NOTE: PGE2 is found in urine, but it is of mixed systemic and renal origin

514531 Prostaglandin E Metabolite ELISA Kit • Converts major urinary and plasma metabolites of PGE2 to a single, stable derivative that is easily quantified • Measure PGEM levels down to 2 pg/ml • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate Sample types not noted may still be assayed but have not been validated by Cayman Scientists

Prostaglandin F2a Item No. Product Features 516011 Prostaglandin F • Measure PGF2a in multiple sample types Prostaglandin F2 (PGF2 ) is produced from arachidonic acid through 2α α α ELISA Kit • Measure PGF levels down to 10 pg/ml the enzymatic reduction of PGH2. The conversion to PGF2α has been 2α shown in many cells including kidney, uterus, and seminal vesicles. The • Incubation : 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm prominent action of PGF2α is luteolysis, as well as and constriction of the trachea and vascular smooth muscle. Binding • Assay 24 samples in triplicate or 36 samples in duplicate of PGF2 has been demonstrated in a variety of tissues, of which the α •  corpus luteum shows the highest activity. Like all of the primary PGs, NOTE: PGF2α has a short half-life in circulation – it is quickly metabolized into 13,14-dihydro-15-keto PGF PGF2α has a very short half-life in the general circulation. The plasma 2α concentration of PGF in humans is <10 pg/ml, and probably no 2α 516671 13,14-dihydro- • Detect 13,14-dihydro-15-keto PGF , a stable more than 1-2 pg/ml. PGF is rapidly metabolized by 15-hydroxy PG 2α 2α 15-keto metabolite of PGF dehydrogenase and both β- and ω-oxidation systems to a variety of 2α Prostaglandin F • Assay 24 samples in triplicate or 36 samples in polar metabolites including 13,14-dihydro-15-keto PGF . In primary 2α 2α ELISA Kit duplicate cultures, the metabolism of PGF is much more limited, and it may 2α • Measure 13,14-dihydro-15-keto PGF levels down to collect in the supernatant medium to easily measured concentrations. 2α 15 pg/ml • Incubation : 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm

Sample types not noted may still be assayed but have not been validated by Cayman Scientists O COOH

O PGF2 Analogs as Ocular Hypotensives PGH2 OH α

Certain metabolically stable analogs of PGF2α act as highly potent agonists PGF Synthase for the FP receptor. In human and animal models of glaucoma, FP receptor agonist activity corresponds very closely with intraocular hypotensive

OH activity. Both 17-phenyl trinor PGF2α ethyl amide, an F-series PG analog, and , an F-series PG ester prodrug, have been approved for use as COOH ocular hypotensive agents.

HO Item No. Product Features OH 516821 17-phenyl • Measure 17-phenyl trinor PGF (also known as PGF 2α 2α 15-hydroxy PGDH trinor ) levels down to 2.6 pg/ml Prostaglandin • Incubation: 18 hours | Development: 90-120 minutes | 15-oxoprostaglandin Δ13-reductase F2α ELISA Kit Read: Colorimetric at 405-420 nm OH • Assay 24 samples in triplicate or 36 samples in duplicate

COOH 516811 Latanoprost • Measure the free acid form of latanoprost HO ELISA Kit • Measure latanoprost levels down to 15.5 pg/ml O • Incubation: 2 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm 13,14-dihydro-15-keto PGF2α • Assay 24 samples in triplicate or 36 samples in duplicate

516761 Fluprostenol • Measure the free acid form of fluprostenol ELISA Kit • Measure fluprostenol levels down to 16 pg/ml • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate

6 Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • www.caymanchem.com FP Receptor Reagents Antibodies/Probes Agonists Antagonists

FP Receptor Polyclonal Antibody Prostaglandin F2α (+)- AL 8810 101802 16010 16765 16735

16,16-dimethyl Prostaglandin F2α (+)-Cloprostenol (sodium salt) AL 8810 isopropyl ester 16750 16766 10113

15(S)-15-methyl Prostaglandin F2α 16-phenoxy tetranor Prostaglandin F2α AL 8810 methyl ester 16743 16760 10008370

Latanoprost Prostaglandin F2α dimethyl amide 16812 10005440 16032

Latanoprost (free acid) Tafluprost (free acid) Prostaglandin F2α dimethyl amine 16811 10005439 16033

17-phenyl trinor Prostaglandin F2α Fluprostenol 16810 16768

17-phenyl trinor Prostaglandin F2α ethyl amide Fluprostenol isopropyl ester 16820 16769

Isoprostanes The isoprostanes are a family of eicosanoids formed by the random oxidation of tissue phospholipids by radicals. 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress.

Item No. Product Features

516351 8-Isoprostane ELISA Kit • Sample Types: Culture Medium | Plasma | Serum | Urine • Measure 8-isoprostane levels down to 3 pg/ml • Measure 8-isoprostane, a biomarker of oxidative stress and antioxidant deficiency • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm • Assay 24 samples in triplicate or 36 samples in duplicate 516360 8-Isoprostane Express ELISA Kit • Sample Types: Plasma | Serum | Tissue Culture Supernatants | Urine | Whole Blood • Measure 8-isoprostane levels down to 10 pg/ml • Measure 8-isoprostane, a biomarker of oxidative stress and antioxidant deficiency • Rapid assay; get results in under 4 hours • Assay 24 samples in triplicate or 36 samples in duplicate

Sample types not noted may still be assayed but have not been validated by Cayman Scientists

For full information on these products and more, visit www.caymanchem.com 7 Prostaglandin Screening ELISA Kit 514012

• Measure total PG levels in tissue culture medium • Measure total PG levels down to 30 pg/ml 100 100

• Assay 24 samples in triplicate or 36 samples in duplicate Evaluate data cautiously

• Incubation: 18 hours | Development: 60-90 minutes | 80 Use data with confidence 80 Read: Colorimetric at 405-420 nm

60 60 ____

----

This assay was developed for screening applications in which 0 the relative amount of PG production for a large number of cell %CV culture samples must be determined. The antiserum used in this %B/B 40 40 assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay. 20 20 Specificity: 0 0 PGE1α 100% PGE 100% 10 100 1,000 2α Prostaglandin (pg/ml) PGF1α 100%

PGF2α 100% Assay Range = 15.6-2,000 pg/ml Sensitivity (defined as 80% B/B0) = ~30 pg/ml PGF α 51.3% 3 Mid-point (defined as 50% B/B0) = ~125-250 pg/ml

The sensitivity and mid-point were derived from the standard curve For a full specificity profile, please go to www.caymanchem.com shown above. The standard was diluted with ELISA Buffer.

Prostaglandin Screening Libraries Put our expertise to work for you. Discover the Cayman difference!

A collection of Cayman’s extensive, highly purified Prostaglandin Screening Library I (96-Well) 10501 prostaglandin compounds conveniently packaged in a • Contains >70 compounds ready-to-use format • Includes the “F-series” prostaglandins and isoprostanes 50 µl 100 µl • Amenable to high-throughput 200 µl screening Prostaglandin Screening Library II (96-Well) 10502 • Cost-effective while providing • Contains >60 compounds compound diversity • Includes the “D- and E-series” prostaglandins 50 µl 100 µl • Full disclosure of plate contents 200 µl • Available in a 96-well Matrix tube rack format as 2 mM stock solutions Prostaglandin Screening Library III (96-Well) 10503 in DMSO • Contains >75 compounds • Includes the “A- and J-series” prostaglandins 50 µl 100 µl 200 µl

8 Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • www.caymanchem.com