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CENPO Antibody (Pab)

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CENPO Antibody (Pab) 21.10.2014CENPO antibody (pAb) Rabbit Anti -Hu man/Mouse/Rat Centromere Protein O (CENP -O) Instruction Manual Catalog Number PK-AB718-5433 Synonyms CENPO Antibody: Centromere protein O, CENP-O, interphase centromere complex protein 36 Description Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma. Quantity 100 µg Source / Host Rabbit Immunogen CENPO antibody was raised against a 16 amino acid peptide from near the center of human CENPO. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 µg/mL. Images Available upon request. References Fukagawa T. Assembly of kinetochore in vertebrate cells. Exp. Cell Res. 2004; 296:21-7. Okada M, Cheeseman IM, Hori T, et al. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat. Cell Biol. 2006; 8:446-57. Hori T, Okada M, Maenaka K, et al. CENP-O class proteins form a stable complex and are required for proper kinetochore function. Mol. Biol. Cell 2008; 19:843-54. Saito A, Muro Y, Sugiura K, et al. CENP-O, a protein localized at the centromere throughout the cell cycle, is a novel target antigen in systemic sclerosis. J. Rheumatol. 2009; 36:781-6. Images Available upon request. Related Products Cat.No. PK-AB718-5433P - CENPO Peptide Cat. No. PK-AB718-1405 - Mouse Kidney Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .
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