Hydrosols of Orange Blossom (Citrus Aurantium), and Rose Flower (Rosa Si on Définitive Du M Définitive On

Home , Satureja thymbra

Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript éieCerutti Céline [email protected] Guinebretiere),(M.-H. [email protected] Saint- France. Domaine 9, CS40509, cedex Avignon l'Aérodrome, 84914 de Agroparc, Site Route Paul, 228, PACA, Recherche de Centre INRA, 9396/ 05Esve t.Alrgt reserved. rights All Ltd. Elsevier 2015 0963-9969/© http://dx.doi.org/10.1016/j.foodres.2015.07.014 or distillation steam The East. Middle the and basin Mediterranean the as used mainly are These (EOs). food oils essential valuable abun- highly less but much dant the of production the for plants aromatic of distillation Introduction 1. Distillation Spoilage Carbohydrate oils Essential water Rose Or Keywords: 2015 July 12 online Available 2015 July 6 Accepted 2015 July 3 form revised in Received 2015 May 7 Received history: Article c b a Labadie Cécile ( blossom orange of Hydrosols ( eeoeeu piaemicrobiota spoilage heterogeneous ril info article Journal homepage Food Research International Ve vgo nvrié M48Scrt tQaiédsPout 'rgn ééae -40 vgo,France Avignon, F-84000 Végétale, d'Origine Produits des Qualité et Sécurité UMR408 Université, Avignon letVeleSS 62 alui,France Vallauris, 06227 SAS, Vieille Albert NA M48Scrt tQaiédsPout 'rgn ééae -40 vgo,France Avignon, F-84000 Végétale, d'Origine Produits des Qualité et Sécurité UMR408 INRA, ⁎ oadamascena Rosa nebl ange -aladdresses: E-mail Végétale, d'Origine Produits des Qualité et Sécurité UMR408 at: author Corresponding yrsl r opout rmtesemdsilto rhydro- or distillation steam the from co-products are Hydrosols damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa ﬂ si vrn usacsi ierneo atisadbvrgsof beverages and pastries of range wide a in substances avoring on définitive du m définitive on os Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de o water som [email protected] Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 [email protected] a a rdrcCarlin Frédéric , , b , c hita Ginies Christian , C Cerutti), (C.

: http://www. C Ginies), (C. anu

and scr [email protected] [email protected] it

oacentifolia Rosa p (201 ub , elsevier.com/locate [email protected] b lié Comment citer cedocument: , 5 c , ), ⁎ e CMGC Renard), (C.M.G.C.

tr n loa Chsbe bevdi l tde yrsl hnsoe nannseiecnanr ncon- In container. non-sterile a in stored when hydrosols studied and all log 7 in priming about observed were been counts a maximal hydrosols, temper- has taminated by ambient °C at proliferation distillation 5 microbial mg/L at during A microorganisms. 0.21 also over by and between nutrients ature carried as varied used likely content be were could carbohydrate these and compounds of effect, the foaming content non-volatile and matter These mg/L, dry mg/L. The 702 hydrosols. 0.38 and blossom C. and mg/L rose orange for 4.0 for mg/L between mg/L 116 677 varied and were hydrosols, hydrosols D. oils rose essential for in mg/L concentrations 205 median hydrosols, The decay. for responsible factors som), rpeyehlaeaet rdc -hnltao.E ocnrtosi yrsl rcl trg r o suf not are storage cold acid, or geranic hydrosols or in concentrations 6-methyl-5-hepten-2-one EO 2-phenylethanol. produce produce to to geraniol acetate phenylethyl as or such compounds, volatile metabolize to able Rhodospirillaceae ouainwscmoe fevrnetlGa-eaiebcei,arne nfu ao genera: major four nas in arranged bacteria, Gram-negative environmental of composed was population aia oa atra on f2log 2 of count bacterial total maximal iini seta isadtemcoit f2 yrslsmlsof spoilage samples hydrosol 22 for of microbiota compo- Reasons the The contaminants. microbiological and of proliferation. oils essential and growth in supporting microbial sition substrates of to nature the subjected essential in found dispersed are be of to Hydrosols g/L have 1 properties. than organoleptic less contain giving They oils plants. aromatic of products hydrodistillation are Hydrosols abstract ilb eesr oisr irba stability. packaging microbial aseptic insure or to preservatives necessary chemical be as will such hurdles Additional stability. microbiological insure to cient Vol. b s / s dan , c ai-éèeGuinebretiere Marie-Hélène , sp.,

irsaurantium Citrus oadamascena Rosa 76 ukodracepacia Burkholderia Fi - 576 , p. na F Carlin). (F.

l upr h rwho a of growth the support ) C Labadie), (C. ver mn hs bacteria, those Among . 586, si / foodres ilr(oeD) and D.), (rose Miller on of the ma on of DOI ope,adpeual w e eeablnigto belonging genera new two presumably and complex, ftreod n loos uha iaoland linalool as such alcohols, and terpenoids of ( and hydrosol miscible from not naturally are separate EOs low concentrations, a higher organ- the At of confers properties. that and g/L) oleptic 1 water than less condensed (usually EO of dissolved of composed amount phase compounds, hydrosol volatile the of part and major the containing phase EO the nonmisciblephases: two produces plants aromatic of process hydrodistillation ae)i il f9.% h ao rdcr r ooc and Morocco are producers major The 99.9%. of yield co- its a blossom of orange in and as products) known water) distillation (also the hydrosol blossom of orange 0.1% the about product, of yield a (in EO ( som sp. rose 2009 Secilmis-Canbay, in & Bosgelmez-Tinaz, geraniol ( and citronellol, hydrosols 2-phenylethanol, and blossom, : : 10.1016/j.foodres.2015.07.014 O noag lso n oehdoosaemsl composed mostly are hydrosols rose and blossom orange in EOs ,adrose and ), 10 irsaurantium Citrus nuscri F/L ete es o odwsdtce.Teioae microbial isolated The detected. was mold nor yeast Neither CFU/mL. ukodravietnamiensis Burkholderia oacentifolia Rosa eno,Cabu,Rsel ae,20;Ulusoy, 2005; Baret, & Russell, Chahboun, Jeannot, pt publis b , c ahrn ...Renard M.G.C. Catherine , 10 F/L hl h rnhpamcpi eomnsa recommends pharmacopeia French the while CFU/mL, .ssp. L. ﬂ hed hed .(oeC.) (rose L. ower amara in irsaurantium Citrus :

ﬂ and enne,Adé aae 2014 Casale, & André, Fernandez, . eut ntepouto fneroli of production the in results L.) 05Esve t.Alrgt reserved. rights All Ltd. Elsevier 2015 © wr eeaaye odtriethe determine to analyzed were owers oopigbu capsulatum Novosphingobium

.Dsilto foag blos- orange of Distillation ). .ssp. L. α trieli orange in -terpineol amara Acetobacteraceae b , c , .(rneblos- (orange L. Pseudomo- were and ). fi - Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve pcrmtr(CSQ21;Siaz,Koo aa) ape were Samples Japan). Kyoto, Shimadzu, (GCMS-QP2010; spectrometer quanti and analyzed were pounds Abr iil A nulse aa.Rs C. Rose data). respectively unpublished t, SAS 300 and Vieille t (Albert 800 about of productions annual with Tunisia, .2%o O n oto h 00tana ol rdcincomes production world annual with t ( Turkey 1000 co-produced and the Bulgaria of from hydrodistillation, most and by EO, of obtained 0.025% usually is France. water) from comes production the D. of Rose Most 0.01%). (about EO in yield centifolia ..Oii n rprto fteaaye hydrosols analyzed the of preparation and Origin 2.1. methods and Materials 2. properties aromatic the products. on the impact of composition its chemical and to conditions, relation storage in and behavior its orange origins, and diverse was rose of work of this microbiota of the aim characterize The to control. microbiological for enough ( high 2015 decay Watt, hydrosol 2014; to al., contributes et that of molds proliferation or about bacteria and stability, microbiological their about human cern the of mL 1 ( in yeast absence and and mold CFU/mL, pathogens total a 20 CFU/mL, than 200 lower than count lower count a bacterial recommend 2011; that total standards al., microbiological et professional with Tornuk ply 2013; 2012 Tornuk, Rusul, & & Bhat, Voon, Ozturk, Sagdic, 2008; Chorianopoulos, lips, 2004; Burt, Haroutoun 2012; Skandamis, al., Giaouris, et Ammar 2007; Al-Turki, f against particular n rnebosm( blossom orange and 0m oue trglrtm nevl.Tohnrdgaso fresh of grams hundred Two C. intervals. rose time regular sampling at by volumes period mL storage 50 three-month a over followed popu- tempera- bacterial were ambient in lations Changes at #8.2). or and #7.2, #8.1) #22.2, and #18.2, (samples #7.1, ture #22.1, #18.1, stored (samples containers sterile °C L 5 10 at into poured and distillation) after a days during packaging few and and/or environment sterile (#7 a in samples maintained (there- not tanks blossom fore collection and storage orange industrial the two in collected and were #8) #22), C. rose and samples Two (#18 period. hydrosol storage samples three-month four a during these, followed been Among have analysis. different chemical microbiological for at separated and aseptically sampled were were sample hydrosol L each of 10 quots ( to storage L and processing 5 of in of times analyzed volumes hydrosols work, all present For the to standards. non-compliance aromatic of or case in microbiological performed, be occasionally may with drosols conformity fresh in distillation of hydrodistillation steam or were by produced were hydrosols hydrosols These practices. examined market Mediterranean and the around Selected and Europe basin. in locations different manufac- different at from turers France) (Vallauris, SAS Vieille Albert by lected ...Etato,identi Extraction, 2.2.1. analysis Chemical 2.2. counts. microbiological of tion n nteogncpaewr bobdo Na on absorbed were phase organic the in ing remain- traces Water Fontenay-sous-Bois, France). International, (VWR liquid successive three by NM 02 oni fErp,2014 Europe, of Council 2012; ANSM, damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si Samplesofcommercialrose( in effects, antimicrobial their for known EOs contain Hydrosols oaiecmonswr xrce rm4m oue fhydrosol of volumes mL 4 from extracted were compounds Volatile on définitive du m définitive on Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de fl wr eecletdbfr itlain(1)frdetermina- for (#17) distillation before collected were owers fl . suulyotie ysemdsilto,wt ut low a quite with distillation, steam by obtained usually is L.) wr( ower tpyoocsaureus Staphylococcus Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 oadamascena Rosa obreptoes( pathogens oodborne .aurantium C. fi

: http://www. ainadquanti and cation .E ocnrtosmyteeoentbe not therefore may concentrations EO ). anu – .Mroe h aehdoosms com- must hydrosols same the Moreover ).

enne ta. 2014 al., et Fernandez iudetatoswt . Lo n-hexane of mL 1.5 with extractions liquid ﬂ wr ( owers scr .damascena R. it al 1 Table a,&Nca,20;Fse Phil- & Fisher 2008; Nychas, & ian, ﬁ

.ssp. L. p (201 dwt a hoaorp mass chromatograph gas a with ed ilr yrsl(aacsrose (Damascus hydrosol Miller) i.1 Fig. ub and elsevier.com/locate .Hwvr hr ssm con- some is there However, ). lié ﬁ Comment citer cedocument: 5 amara .Te,5 Lt 0 Lali- mL 100 to mL 50 Then, ). aino oaiecompounds volatile of cation ), .Ascn itlaino hy- of distillation second A ). e

suooa aeruginosa Pseudomonas i-uzo ta. 2011; al., et Ait-Ouazzou Vol. 2 s / s dan SO ilrand Miller . yrsl eecol- were hydrosols L.) .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. ﬂ 4 ).

wrhdoo ( hydrosol ower VR.Vltl com- Volatile (VWR). 76 Fi - 576 , p. ﬂ wrhydrosols ower na

.centifolia R. l ver Fernandez 586, si / foodres on of the ma on of Rosa L.) DOI : : 10.1016/j.foodres.2015.07.014 .%frpa ra fvltl compounds. than volatile lower of deviations. and areas concentrations mean peak EO for % for 3.4% 5.3% by than estimated lower were was These of area reproducibility analyses The surface and extractions. % replicate extractions two or of mg/L mean the in were expressed concentrations compound vol- of 75% of and compounds 85% atile respectively representing (Merck, Germany), 2-phenylethanol Darmstadt, and Sigma-Aldrich), (all geraniol nerol, n-alkanes of series a injecting (C7 (RI) by NIST/EPA/NIH constituents indices 31 and retention for Experimental determined edition) were 08). (8th (NIST library Library Spectral Spectral Mass Mass Wiley ones the the with were of spectra matches mass spectral experimental transfer Mass of the comparison °C. of by 250 performed and and source °C ion 200 respectively the were of line temperatures The scans/s. 1.7 of ftersos atr fpr linalool, pure of factors response measurement the and of standard, internal Saint- as (Sigma-Aldrich, France) n-hexadecane Quentin-Fallavier, using comparisons, area by ( literature ﬂ the in given values the qimn r niae niais rdcsadfatosaei odcaatr.*indicates locations. * characters. sampling bold process in and are fractions Containers and product. Products italics. the in indicated to are equipment applied processes chemical and physical and ing, 1. Fig. 3 Cad5mnhl ie h assetoee a prtdin operated to the in was °C/min eV spectrometer 70 4 mass at The °C, mode time. impact 50 electron hold the follows: min 5 as and was °C 230 cm/s. temperature 35.5 program of velocity constant oven a The at helium was gas carrier The France). BA oun(0m×02 m 0.5 a mm, with 0.25 separated then × min), m splitless 0.5 (30 after in column opened UBWAX (purge Shimadzu) °C (AOC-5000; 250 at auto-sampler mode an with injected avornet.org – nuscri 3)(uec,Bleot,UA n eete oprdto compared then were and USA) Bellefonte, (Supelco, C30) iga fhdoo rdcinsoigtepouta ifrn tgso process- of stages different at product the showing production hydrosol of Diagram pt publis )tocon – 586 Rosa hed hed ﬁ midenti rm p n rnebosmhdoos Volatile hydrosols. blossom orange and sp. in :

ﬁ ain Quanti cation.

www.pherobase.com μ m )(neci,Montluçon, (Interchim, m) / z α ag 29 range triel citronellol, -terpineol, fi ain a done was cations – 5 taspeed a at 450 and www. 577 Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript 578 iae5 Lsmlso reedidhdooswsdsovdi Lof mL 1 Plus in dissolved Milli-Q was hydrosols freeze-dried of samples mL 50 licate noa9-elmcolt GenrBoOe oraou,Fac) A France). Courtaboeuf, Bio-One, (Greiner an microplate in 300 96-well temperature a vortexing, room into s to cooled 10 then After and bath. Maharashtra, min, ice (Lab-line, 15 for bath °C dry 95 a at in India) heated microtubes, all in added ihn2 i olwn etn,ie,bfr ntaino pronounced a coloration. of initiation in decrease before i.e., heating, following min 20 within Safas) (Xenius, measurements spectrophotometric by determined was A the (Fisher reaction, solution of phenol min 30 g/L After 50 a of mL Scienti 0.5 USA), Plus Milli-Q Billerica, of mL Millipore, 0.5 in hydrosol again mL solubilized 50 each of samples volumes freeze-dried replicate three of residues the on ( method sulfuric-acid (Merck). 7.01) pH and 4.00 (pH buffers prepared freshly Toledo, with calibrated (Mettler was meter meter pH pH The Switzerland). Schwerzenbach, a and France) Tanneries, (Hanna, electrode concentrations acid amino and carbohydrate pH, of Determination 2.2.2. ( blossom orange of Description 1 Table rm00 / o00 / fascoewtrslto (Sigma-Aldrich) solution water sucrose a of g/L (R 0.08 set to was g/L curve 0.01 calibration from A Monaco). Safas, (Xenius, measurement ric lrc)i il- Plus Milli-Q in Aldrich) calibration, external For time. same fi the at prepared were samples 2006 Chen, the & Weng, Lin, ( Sun, 2000; Frutos, ones published previously from K o nw.B:Blai;F:Fac;M:Mrco N uii;T:Turkey. TR: Tunisia; TN: Morocco; MA: France; FR: Bulgaria; BG: known. not NK: ihdieraet(im-lrc)i il- Plus Milli-Q in (Sigma-Aldrich) 200 Then, reagent microtube. Ninhydrine screw-capped a into ferred Journal homepage Food Research International Ve a ocnrto agdfo .4gLt .7gLo lcn(Sigma- glycin of g/L 0.07 to g/L 0.04 from ranged concentration nal a b oacentifolia Rosa yrslSample Hydrosol oadamascena Rosa irsaurantium Citrus 2 ape nlzda eea ieitrasdrn -ot storage. 3-month a during intervals time several at analyzed Samples abhdaecnetain aebe eemndb h phenol the by determined been have concentrations Carbohydrate pH a with temperature ambient at measured were pHs hydrosol The mn-cdcnetainwsdtrie sn ehdadapted method a using determined was concentration Amino-acid aoaoy(letVeleSS distillation. SAS) Vieille (Albert Laboratory 0.99). = damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si fi on définitive du m définitive on ,Ilic,Fac)ad25m fpr ufrcai (VWR). acid sulfuric pure of mL 2.5 and France) Illkirch, c, Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de ™ ae.A400 A water. Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 uos ils aitn ees mt,1956 Smith, & Rebers, Hamilton, Gilles, Dubois, 16 17 15 14 13 12 # 11 22.1 21.2 18.3 18.2 18.1 10 22.2 21.1 20 19 ™ ;21;T ipeN trg akN;9mnh o sterile Not sterile Not sterile Not sterile Not sterile Not months 9 NK; months 3 Ambient; months 3 NK; days 20 NK; months 3 NK; tank Storage tank Storage tank Storage tank Storage tank Storage NK NK NK NK NK Simple Simple Simple Simple Simple TN 2013; A; TN 2013; D; TN 2013; C; FR 2013; B; TN 6.1 2013; A; 5 4 3 2 1 9 8.2 8.1 7.2 7.1 6.3 6.2

a a,b : http://www. irsaurantium Citrus ae (R water a a a a a a a a a a a a a a μ lqo ftedsovdrsdewstrans- was residue dissolved the of aliquot L anu

485 scr a eemndb spectrophotomet- by determined was 2 rts ord,PrzLrno & Perez-Lorenzo, Torrado, Frutos, μ ,9.Ec eiu ftrerep- three of residue Each 0,99). = it ;21;BG 2013; K; ;21;TR 2013; J; ;21;MA 2013; I; ;21;BG 2013; H; ;21;FR 2013; B; rdcincountry production production; of year Manufacturer; ;21;MA 2013; G; ;21;TN 2014; D; ;21;FR 2014; B; FR 2014; B; FR 2014; B; FR 2014; B; FR 2012; B; FR 2012; B; FR 2013; B; FR 2013; B; FR 2013; B; FR 2013; B; FR 2014; F; TN 2014; E; TN 2014; E; TN 2014; D; TN 2014; D; FR 2014; B; FR 2014; B; FR 2014; B; fec apewstransferred was sample each of L

p (201 ,rs .( D. rose ), ub elsevier.com/locate lié Comment citer cedocument: 5 .Teclbaincreand curve calibration The ). ), e

Vol. oadamascena Rosa s / s dan .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C.

76 μ f2 (wt/vol) 2% of L ™ Fi - 576 , p. ™ ae (Merck water na

ae was water ,adrs .( C. rose and ), Simple Simple Simple Simple Simple distilled double or Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Simple Double Double Double l ver 586, si / foodres on of the ma on of 570 ) DOI oacentifolia Rosa NK NK NK NK NK itainSampling Filtration NK NK Yes Yes No No Yes Yes Yes Yes Yes No No No No No No Yes No No eehdoitle ntelbrtr nrsetvl 0m,5 mL, 50 mL, Plus 50 Milli-Q respectively of in mL 100 laboratory and the in hydrodistilled were (Sigma-Aldrich) sucrose and (Sigma-Aldrich) glycin France), Beauvais, compounds organic of solutions of distillation Laboratory 2.2.3. ikdrose picked time storage with changes and counts Microbiological 2.3. The °C. 73 ( the at methods using described determined previously oven were solution sucrose an the carbohy- in in concentration the drate and solution condensate drying solution glycin extract days the in yeast three content amino-acid the after of determined (condensate) was in content product weight dry distillation The cylinder. still the graduated a prod- a Distillate in stirrer, condenser. collected were magnetic a ucts and a thermometer, with a with equipped equipped mantle head heating a in heated gr(ikrDansis ogta get to with Diagnostics) complemented PCA, (Biokar fold-diluted agar 10 onto water demineralized spread- 100 by ing performed (CFU) were hydrosols units in colony-forming bacteria mesophilic of aerobic Counts of molds. and mesophilic yeasts aerobic and respectively, (PCA) bacteria enumerate, Agar (YGC) to agar Count Diagnostics), Chloramphenicol Plate (Biokar Glucose Yeast onto and spread Diagnostics) were (Biokar dilutions Stomacher® decimal a 100 priate and using s), 60 water × (2 UK) demineralized Worthing, (Seward, blender sterile 400 mL 100 in blended n onswr eodd onso irba el nhdooswere hydrosols in cells microbial of Counts recorded. col- before were days counts two for ony not °C (data 30 at PCA incubated were on dishes Petri than All DPCA shown). on recovery better a showed consistently speci otherwise unless (DPCA), : : 10.1016/j.foodres.2015.07.014 ouin t2 / fatltcyatetat(ikrDiagnostic, (Biokar extract yeast autolytic of g/L 20 at Solutions irba ouainwas population Microbial yrslsamples. hydrosol ) nuscri μ fteaporaedcmldltoso yrsl nsterile in hydrosols of dilutions decimal appropriate the of L fl wr.Trerpiae f1 rs oeC. rose fresh g 15 of replicates Three owers. pt publis trg tank Storage trg tank Storage trg tank Storage trg tank Storage tank Storage location trg tank Storage tank Storage trg tank Storage tank Storage Florentine Florentine tank Storage tank Storage tank Storage of End of End Florentine days 3 °C; 20 days Florentine 3 °C; 5 tank Storage tank days Storage 10 °C; 20 tank days Collection 10 °C; 5 tank Collection tank Storage tank Collection tank Collection – 586 fi fi tain2 C month 1 °C; 20 month 1 °C; 5 ltration ltration fl fl fl fl hed hed s 0°;6h 6 °C; 20 h 6 °C; 5 ask ask h 7 °C; 20 ask h 5 °C; 5 ask ™ in ae,uiga50m distillation mL 500 a using water, fi : eto 2.2.2 Section s eemndi apeo freshly of sample a in determined rst

ﬁ d rvostssi u laboratory our in tests Previous ed. K 4 NK; K 4 NK; K 4 NK; K 4 NK; days 4 °C; 5 eoeanalysis before duration storage (°C); temperature Storage K 4 NK; months 2 ambient; 0°;1month 1 °C; 20 month 1 °C; 5 months 13 NK; months 13 NK; month 1 °C; 5 days 7 °C; 20 days 7 °C; 5 days 10 °C; 5 ﬁ a 2gLaa concentration agar g/L 12 nal

– – – – – months 6 months 6 months 6 months 6 months 6 ). μ fteappro- the of L fl wr were owers o sterile Not o sterile Not o sterile Not o sterile Not o sterile Not o sterile Not conditions Storage o sterile Not o sterile Not sterile Not Sterile Sterile Sterile Sterile o sterile Not sterile Not sterile Not sterile Not sterile Not sterile Not sterile Not sterile Not o sterile Not sterile Not sterile Not sterile Not fl ask Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve Pseudomonas and multivorans ae,Sizrad.Gl eesandwt 20 with stained LE, were (LonzaSeakem® Gels Switzerland). agarose Basel, g/L 10 with mixed Germany) Heidelberg, Tris of gel electrophoresis 5 a in band size ampli USA). Carlsbad, Technologies, positive (Life thermocycler A 9700 PCR a of use the ( 2001 described previously as GG(A,C)-TAC-CTT- GTT-ACG-A(T,C)T-TC) MOD: (RD1 and GTT-TGA-TC(A,C)-TGG-CTC-AG) rrs at at stored heated then and was min, sample 15 the for °C and 65 added was mg/mL) (0.5 in Deutschland) suspended and dried, and (Sigma-Aldrich), 100 retrieved ethanol was 70% precipitate DNA with The washed microtube. aqueous clean the a to into added phase was × (Sigma-Aldrich) (17,000 ethanol centrifugation absolute After freezing extraction. the along all ice 600 isosre nDC eecmoe fbcei.Te eethen were stored and They picked at then and bacteria. purity, of for DPCA composed on times three were subcultured DPCA on observed and magni nies size ×1000 for at microscopy observed phase-contrast and by se- shape differences, were morphological counts, their microbiological for for used lected plates DPCA on grown ones identi and Isolation 2.4. BX50 magni Olympus ×400 an at and microscope phase-contrast chamber counting Malassez a using performed ooissbutrdo DPCA. on subcultured colonies hi ifrne n4 Cgot aaiy eoyi n eaiaeac- gelatinase NO and tivity, hemolysis capacity, growth °C 42 in differences their PCR buffer. electrophoresis (0.5×) TAE puri in were V products 100 after at read migration were min results 30 and France), Illkirch, Europe, Biomedicals (MP atevle S)i ce-apdmcoue,adte ie to mixed then and microtubes, screw-capped 25 in USA) Bartlesville, 0.1 with grinded and water sterile ultrapure follows. as is cedure non-discriminative pro- Detailed done. with was characterization phenotypic genus a typing sequence, same partial a molecular of a species by ferentiate dif- typed To selected. was were clone each of sample strain representative One (M13-PCR). hydrosol same the from similar with strains isolates, clonal ( RNA ribosomal 16S by lyzed ain fhdoo atrawr hnpromdb oprsno the partial of comparison resulting by performed then were bacteria hydrosol of cations and (5′ S6 primer the using 3730xl, GCGGCTGCTG-3 ABI Sanger a in Germany) yi hrceiainwspromdt ifrnit 5srisof strains 15 differentiate to performed Burkholderia was g/L characterization 15 typic with mixed buffer marker weight TAE 20 mL with stained 100 and agarose of made gel electrophoresis ( library. BLAST nucleotide NCBI the on lata unbeir gynTe 2003 Nguyen-The, & Guinebretiere damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa − si μ eewsampli was gene The prxmtl he ofu ooisprhdoo ape rmthe from sample, hydrosol per colonies four to three Approximately N a xrce rmpuri from extracted was DNA were and hydrosols from isolated were bacteria 71 total In atra lnsaogioae eedtce yM1 PCR M-13 by detected were isolates among clones Bacterial , oimddclslae(0 /) 100 g/L), (200 sulfate dodecyl sodium L .panacis P. on définitive du m définitive on μ μ 0° na40m/ lcrlslto SgaAldrich). (Sigma solution glycerol mL/L 400 a in °C 80 ukodraambifaria Burkholderia ihmnrdfeecsi h ubro yls( 5 and 35) = (n cycles of number the in differences minor with ) ukodracepacia Burkholderia fpr hooom(l im-lrc) ape eekp on kept were Samples Sigma-Aldrich). (all chloroform pure of L lrpr trl ae.RAe(oh igotc Mannheim, Diagnostic, (Roche RNAse water. sterile ultrapure L Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de rrs 2 euto and reduction eecrepnigt oiin o14 on 1548 to 9 positions to corresponding gene n 8srisof strains 18 and ) p aigtesame the having sp. pce eedfeetae ygltns n hemolysis and gelatinase by differentiated were species ; Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 ‘ .veronii P. mr Ladder Smart ′ μ rrs ,position518 fi oueo ahapio eoie noa10mL 100 a onto deposited amplicon each of volume L ainwscekdb eeaino h expected the of revelation by checked was cation fi fi eune othe to sequences duigtomodi two using ed dadsqecdb ACBoeh (Constance, Biotech® GATC by sequenced and ed

ﬁ : http://www. aino bacteria of cation β and anu glcoiaeatvt ( activity -galactosidase –

,and acetate ; μ ’ suooa extremaustralis Pseudomonas rrs scr Ergne,Srig egu) pheno- A Belgium). Seraing, (Eurogentec, tiimboie sn h molecular the using bromide, ethidium L – u lata Bu. Pseudomonas 3 of 534 Burkholderia eesqecn.Te,t determine to Then, sequencing. gene ) rrs ﬁ ukodravietnamiensis Burkholderia it

p dclne,sseddi 400 in suspended colonies, ed (201 – eeprilsqec ( sequence partial gene rrs DA(A)bfe 05)(Serva, (0.5×) buffer (TAE) EDTA − .Famnswr eeldo an on revealed were Fragments ). ub rrs shrci oirrs coli Escherichia , elsevier.com/locate 0° ni ute analysis. further until °C 20 eeprilsqec isolated sequence partial gene lié ﬁ Comment citer cedocument: u cepacia Bu. eesqecso yestrains type of sequences gene 5 dpies(D O:AGA- MOD: (FD1 primers ed ), ﬁ e

cation. Vol. pce eeidenti were species s / s dan p ( sp. μ μ iiabas(i Spec, (Bio beads silica m NaClO L μ .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. fehdu bromide ethidium of L

suooa migulae Pseudomonas aleee l,2009 al., et Vanlaere 76 unbeir tal., et Guinebretiere Fi - 576 , p. and ﬁ 4 na ain l colo- All cation. ee.Identi gene). 5mlL,and mol/L), (5 ailssubtilis Bacillus

l Burkholderia ,uigpure using ), Burkholderia -GTATTACC ver g ,1m of mL 1 ), ; fi rrs 586, u lata Bu. si s ana- rst / fi foodres on of the ma on of dby ed gene μ Lof DOI fi ). - : : 10.1016/j.foodres.2015.07.014 aey10 a mately at inoculated was culture pure vious ciiy( activity ..Cagsi pro in Changes 2.5. days. seven for incubated vis- of plates formation DPCA by on tested colonies was °C ible 42 at capacity using Growth Pont °C, control. Le 30 positive Columbia, at (BD incubated plates France) agar Claix, blood de horse on performed were tests ( peptone and g/L), (3.0 extract yeast autolytic of concentrations increased and France), Marnes-la-Coquette, previously as dishes Petri by on described performed cell were as tests g/L 8.5 Gelatinase (VWR) medium. NaCl using France) l'Etoile, Marcy 20 (Biomerieux, API® E on tested were metabolism glucose and activity, galactosidase t3 Cudrsaiga 0 p sdsrbdin described as rpm 200 at shaking incubation under 10-day °C 30 after at and counts (t0), time microbial inoculation and at determined compounds were volatile in Concentrations allow #8.2, growth. to hydrosol water contaminated demineralized in heavily suspended the and of washed mL 100 from obtained let 20 with sup-plemented was hydrosol blossom Orange hydrosols. C. rose or blossom orange and bacteria mso rwhmdu opsdo 0m/ rpoe(Biokar and Diagnostic) tryptone (Biokar mg/L extract 50 mg/L yeast 10 of autolytic vol- composed mg/L mL 25 medium 5 replicate Diagnostic), growth three into a rpm of 200 at umes shaking under °C over- 30 subcultured at were night temperature) ambient at stored #8.2 (sample ..Saitclanalysis Statistical 2.6. ed bevdi rvossuisadidsra ape frs and rose of samples industrial and studies and previous 11.2% in observed pro between ready Volatile varied hydrosols. C. rose citronellol rose in of in 80.3% 58.4% and amounts 10.7% and between hydrosols, and D. 30.7% and respec- 0.6% varied 2-phenylethanol between and tively 6-methyl-5- geraniol hy- of of blossom areas orange surface areas in drosols, 22.0% surface and 0.01% example, between varied For hepten-2-one greatly samples. compounds few among a of varied 21.2% Concentrations at respectively). areas 20.9% surface 11.3% at (median and geraniol areas and surface respectively), (median 24.6%, citronellol and D. rose respectively), for C., 45.6% rose and and 25.0% were at compounds areas major surface The and hydrosols. (median hydrosols, C. 2-phenylethanol rose D. for rose 482] for [100; 597] mg/L [132; 116 mg/L value; 205 [minimal were (median value]) hydrosols maximal rose the respectively). of 1.6%, concentrations and EO 2.9%2.5% The 4.2%, at areas surface (median and geraniol and phenylacetonitrile, nerol linalool anthranilate, methyl and extent respectively), were lesser 23.7%, a and compounds to 44.1% major at areas The surface (median 866]. terpineol was value]) [276; maximal mg/L value; [minimal 677 (median work this in analyzed are basin Mediterranean the in presented around and Europe in at locations produced hydrosols different C. rose eight and D., rose six blossom, orange nine compounds Volatile 3.1.1. composition Hydrosol 3.1. discussion and Results 3. France). Mann Paris, (Addinsoft, software the XLstat with the evaluated was samples two Puri h signi The Ocnetain n ecnaeae f3 oaiecmonsof compounds volatile 31 of area percentage and concentrations EO nuscri u vietnamiensis Bu. fi D dclne of colonies ed vnv ta. 02 ake l,2005 al., et Park 2002; al., et Ivanova ()guoe(im lrc) hn ahrpiaeo h pre- the of replicate each Then, Aldrich). (Sigma -(+)-glucose 3 pt publis F/Lit Lvlmsof volumes mL 5 into CFU/mL fi mt n ode (1958) Goodner and Smith al 2 Table ac ftedfeecsi h ttsia itiuin of distributions statistical the in differences the of cance fi – μ e fvltl opudi yrsl ncltdwith inoculated hydrosols in compound volatile of les 586 h Ocnetaino rnebosmhydrosols blossom orange of concentration EO The . faha-rae 2 i t11°)bceilpel- bacterial °C) 121 at min (20 heat-treated a of L hed hed sri AF_8 sltdfo n hydrosol one from isolated IA_FO_28) (strain oopigbu capsulatum Novosphingobium in :

. /)(im-lrc) Hemolysis (Sigma-Aldrich). g/L) 5.0

sn eai t4. / (Biorad, g/L 40.0 at gelatin using , fi fi e orsodt h nsal- ones the to correspond les a ocnrto fapproxi- of concentration nal ailscereus Bacillus fi – trseiie (0.22 lter-sterilized hte etadusing and test Whitney etos221ad2.3 and 2.2.1 Sections .NO ). sri IA_FO_33) (strain 2 TC47 as ATCC14579 .capsulatum N. reduction, μ m) 579 α β - - . Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve damascena and Rosa centifolia) support the growth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosols of orange blossom (Citrus aurantium), and rose flower (Rosa si on définitive du m définitive on

Labadie, C., Ginies, C., Guinebretière, M. H.,Renard, C., Cerutti, C., Carlin, F.(Auteur de

o ahhdoo n ahvltl opud niae signi a indicates * compound, volatile each and hydrosol each For sn ok n ieaueadidsra aaat data industrial and literature and work, esent pr the in analyzed samples between difference cant hte test). Whitney (Mann 0.05 P ﬁ – b e

Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 xeietlrtninidxo oa oun(UBWAX). column polar on index retention Experimental

n letVeleSSupbihddata. unpublished SAS Vieille Albert and (2005) Ur Shoaib & Khan Aslam

n letVeleSSupbihddata. unpublished SAS Vieille Albert and , (2014) al. et Mahboubifar , (2009) al. et Ulusoy b

n letVeleSSupbihddata. unpublished SAS Vieille Albert and ,

eno ta.(2005) al. et Jeannot : http://www. a

anu

0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 3.2] 0.01; [ 0.8 1.3] 0.01; [ 0.8 Indole 2455 0.01] 0.01; [

b b b b b b b b b b b b b b

.2 0.4] 0.02; [ 0.2 0.01] 0.01; [ 0.01 0.5] 0.01; [ 0.01 0.2] 0.01; [ 0.01 2.7] 0.01; [ 0.2 1.1] 0.01; [ 0.1 Farnesol 2429

b b b b b b b b b b

⁎ scr

.1 3.9] 0.01; [ 0.01 0.2] 0.01; [ 0.01 2.1] 0.01; [ 0.01 0.1] 0.01; [ 0.02 2.0] 0.01; [ 0.01 1.1] 0.01; [ 0.1 acid Geranic 2299 b b b b b b b b b b

4.2 anthranylate Methyl 2250 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 8.6] [1.0; 3.4 5.3] [2.8; b b b b b b b b b b b b it

(201

0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 1.4] 0.01; [ 0.01 1.6] 0.01; [ 0.1 4-Vinylguaiacol 2211 0.01] 0.01; [ 0.01 0.01] 0.01; [

b b b b b b b b b b b b b b b

. 04 4.0] [0.4; 1.8 3.5] [1.4; 3.1 8.6] 0.01; [ 3.2 10.9] [0.1; 2.1 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 Eugenol 2177 b b b b b b elsevier.com/locate

lié

Comment citer cedocument : 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 1.4] 0.01; [ 0.2 1.6] 0.01; [ 0.03 03Nerolidol 2053 5 b b b b b b b b b b b b b b

.1 .]24[.;3.8] [2.0; 2.4 4.3] 0.01; [ 1.9 5.6] [0.7; 2.7 0.01] 0.01; [ 0.01 1.3] 0.01; [ 0.04 eugenol Methyl 2024 . 01 2.3] [0.1; 1.3

b b b b

⁎ Vol.

s / s dan

0.01 0.01] 0.01; [ 0.01 0.1] 0.01; [ 0.1 Jasmone 1953 0.01; [ 0.6 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 1.0]

b b b b b b b b b b b b b b ⁎

.1 0.3] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 5.0] 0.01; [ 0.1 4.5] [0.6; 2.9 Phenylacetonitrile 1940

b b b b b b b b b b b b

⁎

76 . 02 .]2[.;79 50[07 03 28[33 54 56[42 54.2] [24.2; 45.6 85.4] [13.3; 32.8 80.3] [10.7; 25.0 7.9] [0.1; 2 5.2] [0.2; 1.5 ethanol 2-Phenyl 1925 42[00 79.0] [40.0; 54.2

586 ⁎

–

.1 .]04[.;0.7] [0.1; 0.4 1.1] 0.01; [ 0.1 1.7] [0.1; 0.3 0.01] 0.01; [ 0.01 0.02] 0.01; [ 0.01 alcohol Benzylic 1886 0.01] 0.01; [ 0.01

b b b b b b b b b Fi - 576 , p. ⁎

. 01 .]36[.;78 12[.;3.]1. [ 18.7 30.7] [0.6; 21.2 7.8] [0.1; 3.6 8.0] [0.1; 1.6 Geraniol 1857 . 27 15.8] [2.7; 7.4 .1 13 13[.;13.5] [6.4; 11.3 31.3] 0.01; na b

0.01] 0.01; [ 0.01 0.8] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.2] 0.01; [ 0.01 0.1] 0.01; [ 0.01 acetate 2-Phenetyl 1824 .1 0.5] 0.01; [ 0.2

b b b b b b b b b b b b l ⁎

ver

. 01 .]23[.;35 08[.;15.1] [1.3; 10.8 3.5] [0.1; 2.3 2.8] [0.1; 2.5 Nerol 1809 . 06 5.7] [0.6; 4.0 5.1] [2.0; 3.8 16.5] 0.01; [ 9.3 b

.1 .]00 00;00]2. 1.;2.]1. 23 87 46[12 58.4] [11.2; 24.6 38.7] [2.3; 17.1 27.3] [11.7; 20.9 0.01] [0.01; 0.01 0.7] 0.01; [ 0.1 Citronellol 1775 65[.;22.8] [3.6; 16.5

b 586,

⁎ si

foodres

0.01; [ 0.02 0.01] 0.01; [ 0.01 1.8] 0.01; [ 0.1 1.4] 0.01; [ 0.1 acetate Geranyl 1768 1.9] .1 . [0.04; 0.1 0.01] 0.01; [ 0.01 0.3] b b b b b b b b b the ma on of

0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.4] 0.01; [ 0.1 0.8] 0.01; [ 0.1 acetate Neryl 1765 b b b b b b b b b b b b b b

DOI

0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.2] 0.01; [ 0.1 Geranial 1745 0.01] 0.01; [ 0.01 1.3] 0.01; [ 0.3 .1 3.5] 0.01; [ 0.2

b b b b b b b b b b b b ⁎ ⁎

70Apatriel2. 1.;3.]2 1.;3.]09[ 0.9 36.3] [11.3; 22 38.0] [10.7; 23.7 terpineol Alpha 1710 .1 .]06[.;1.]04[ 0.4 11.4] [0.1; 0.6 4.7] 0.01; [ 1.3 4.9] 0.01; .1 1.7] 0.01; b b b

: :

.1 0.5] 0.01; [ 0.1 1.0] 0.01; [ 0.1 1.0] 0.01; [ 0.1 1.8] 0.01; [ 0.7 0.01] 0.01; [ 0.01 0.1] 0.01; [ 0.03 Neral 1690

b b b b b b b b 10.1016/j.foodres.2015.07.014

0.01; [ 0.01 0.01] 0.01; [ 0.01 0.1] 0.01; [ 0.04 0.01] 0.01; [ 0.01 2.6] 0.01; [ 0.03 acetaldehyde 2-Phenyl 1670 0.01] 0.01; [ 0.01 1.2] b b b b b b b b b b b b

. 04 .]15[.;3.5] [0.1; 1.5 2.6] [0.4; 1.7 4-Terpineol 1613 .1 1.9] 0.01; [ 1.1 .1 1.0] 0.01; [ 0.2 0.4] 0.01; [ 0.1 0.9] 0.01; [ 0.4

b b b b nuscri ⁎

41[49 49 54[31 73 . 06 7.1] [0.6; 1.9 67.3] [23.1; 45.4 64.9] [24.9; 44.1 Linalool 1563 . 00;5.0] [0.03; 1.0 4.6] [0.4; 2.0 8.0] [1.0; 3.1

53Benzaldehyde 1543 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 7.3] 0.01; [ 0.4 0.1] 0.01; [ 0.01

b b b b b b b b b b b b b b ⁎

Lnlo xd . 07 .]24[.;5.7] [0.5; 2.4 5.7] [0.7; 3.4 oxide -Linalool trans 1483 .1 0.2] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.5] 0.01; [ 0.01 0.01] 0.01; [ 0.01

b b b b b b b b b b pt publis

436Mty--etn2o . 00;2.2] [0.02; 0.4 6-Methyl-5-hepten-2-ol 1473 0.01] 0.01; [ 0.01 0.6] 0.01; [ 0.04 0.01] 0.01; [ 0.01 10.0] 0.01; [ 0.04 10.0] 0.01; [ 0.01

b b b b b b b b b b e ⁎

Lnlo xd . 14 .]4[.;8.8] [1.0; 4 9.6] [1.4; 5.7 oxide -Linalool cis 1450 .1 0.4] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.7] 0.01; [ 0.01 0.01] 0.01; [ 0.01 b b b b b b b b b b

3Hxnl00 [ 0.02 -3-Hexenol cis 1398 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 0.1] 0.01; b b b b b b b b b b b b b b b b

30Rs oxide Rose 1360 compounds .1 .]03[ 0.3 1.8] 0.01; [ 1.2 0.01] 0.01; [ 0.01 0.01] 0.01; [ 0.01 .1 0.4] 0.01; [ 0.2 2.0] 0.01; [ 0.7 1.8] 0.01;

b b b b b b b b b b ⁎ hed

386Mty--etn2oe02[.1 20 . 00;1.]02[ 0.2 10.5] [0.03; 1.8 22.0] [0.01; 0.2 6-Methyl-5-hepten-2-one 1348 aromatic of % area and RI .1 5.4] 0.01; .1 .]02[.;1.5] [0.1; 0.2 1.4] 0.01; [ 0.01 0.04] 0.01; C. Labadie et al. / Food Research International 76 (2015) 576 [ 0.01

b b b b d ⁎

d in

(mg/L)

seta i concentration oil Essential 7 26 6]47[1;17]25[3;57 4 4;80 1 10 8]15[0 519] [50; 165 482] [100; 116 830] [41; 240 597] [132; 205 1075] [210; 477 866] [276; 677 ein[i;mx ein[i;mx ein[i;mx ein[i;mx ein[i;mx ein[i;max] [min; Median max] [min; Median max] [min; Median max] [min; Median max] [min; Median max] [min; Median

= n=43 n=9 n=6 n=8 n=24 n=15

a b c

data data data

hswr ieaueadindustrial and Literature work This hswr ieaueadindustrial and Literature work This hswr ieaueadindustrial and Literature work This

irsarnimRs aacn oacentifolia Rosa damascena Rosa aurantium Citrus

Hydrosols

so 1vltl opud foag lso ( blossom orange of compounds volatile 31 of es percentag area and concentrations oil Essential irsaurantium Citrus ,adrs .( C. rose and ), damascena Rosa ( D. rose ), oacentifolia Rosa yrsl,adcmaio ihltrtr n nutildata. industrial and literature with comparison and hydrosols, ) al 2 Table 580 Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve a ls oteMCagainst MIC the to close ( was bacteria Gram-negative than 2004 bacteria Gram-positive against ( mg/L 200 2 gLad90m/ ( mg/L 900 and mg/L 225 Salmonella .monocytogenes L. coli E. against 2-phenylethanol of MIC albicans and (MIC ( mg/L 200 against of activity inhibitory monocytogenes For an Listeria properties. exhibits antimicrobial linalool their for example, known are hydrosols blossom ( data) unpublished 2009 SAS al., Vieille Albert et Ulusoy 2014; Javidnia, & Shahabipour, Mahboubifar, 2005; reported previously also were ( example for samples among hydrosols amounts in amounts 6-methyl-5-hepten-2- high linalool, in in variations one, and also studies, were previous in hydrosols, analyzed sp. rose geraniol both and and citronellol in linalool 2-phenylethanol, i.e., hydrosols, work, blossom this orange in analyzed samples the in conditions. opsto,p f n atra onsi rnebosm( blossom orange in counts bacterial and of, pH Composition, 3 Table of strains were concentrations the signi in differences and hydrosols blossom orange gra ta. 05 sa hn&Soi r 05 eno tal., et Jeannot 2005; Ur, Shoaib & Khan Aslam 2005; al., et Agarwal damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food d c b a oacentifolia Rosa oadamascena Rosa irsaurantium Citrus yrslSample Hydrosol r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si ape nlzda eea ieitrasdrn -ot storage. 3-month a during intervals time several at analyzed Samples See h ao opud otie nteE hs frs n orange and rose of phase EO the in contained compounds major The en±s,n=3aayia elctso ahsample. each of replicates analytical 3 = n sd, ± Mean aoaoy(letVeleSS itlain T o etd D o determined. not ND, tested; not NT, distillation; SAS) Vieille (Albert Laboratory .monocytogenes L. on définitive du m définitive on ﬁ .Lnlo ocnrto bevdi rnebosmhydrosol blossom orange in observed concentration Linalool ). N and at( cant Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de al 1 Table 0 gL gis te tan of strains other against mg/L) 900 ,and Salmonella .coli E. h ta. 2011 al., et Zhu P Typhimurium, . b α acaoye cerevisiae Saccharomyces Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 i-uzo ta. 2011 al., et Ait-Ouazzou .5 nyfrafwcmons h ao compounds major The compounds. few a for only 0.05) triel irnlo,grno,ad2pey ethanol 2-phenyl and geraniol, citronellol, -terpineol, , .aureus S. ( ut 2004 Burt, yhmru s50m/,ad10 gLagainst mg/L 1000 and mg/L, 500 is Typhimurium ,

Salmonella : http://www. ihmnmlihbtr ocnrtos(MICs) concentrations inhibitory minimal with oetn ta. 1999 al., et Cosentino anu 17 # 16 15 14 13 21.1 20 12 11 22.2 22.1 21.2 19 18.3 18.2 18.1 10

,and 9 8.2 6.3 . TN . 6.6 4 6 4.6 6.2 5.3 4.1 6 4.9 6.4 NT NT NT NT NT NT 3.2 ± 95 6.7 ± 384 21 ± 319 12 ± 253 4.5 4.7 5.6 4.2 4.8 6.1 5 4 3 2 1 8.1 7.2 7.1 6.2 ;teMC of MICs the ); .aureus S. c c,d .coli E. .Esso etratbceilactivity antibacterial better show EOs ). scr c c c c c c c c c c c c c c a a cereus Ba. it .coli E. Typhimurium,

p (201 and ub al 2 Table ,and elsevier.com/locate .monocytogenes L. lié and Comment citer cedocument: 5 ,o 0 gLaantsome against mg/L 900 of ), ( oetn ta. 1999 al., et Cosentino ), e n oihbtr activity inhibitory no and , α .coli E.

5.4 4.7 4 6.4 6.7 7 4.8 4.7 4.4 4 4.1 4.1 6.5 7 6.8 5.6 5.9 5.6 4.8 4 6.4 7 7.1 6.9 4 HDymte (mg/L) matter Dry pH Vol. asoi solanacearum Ralstonia s / s dan trielagainst -terpineol ). a cereus Ba. ;grno' I against MIC geraniol's ); irsaurantium Citrus .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. .aeruginosa P.

, 76 , aureus S. Fi - 576 , p. na α

NT NT NT NT NT NT NT NT NT 94 ± 641 NT NT . 2.6 ± 4.0 NT NT NT NT 17 ± 164 NT NT NT 13 ± 281 NT 88 ± 702 25 ± 348 r between are ( trielin -terpineol l Ai ver .coli E. , cereus Ba. t-Ouazzou ,rs .( D. rose ), , 586, Candida si / foodres .coli E. ;the ); on of the ma on of Burt, and oaDamascena Rosa DOI is ; , b : : 10.1016/j.foodres.2015.07.014 neae oae n ero rdcin n otp fdistilled of type to and production, of year and area to unrelated pH and compounds Nonvolatile 3.1.2. rose in shown already as These solutions. compound suf pure not of likely are concentrations MICs reported the than lower fold nebosm oeC n oeD. rose and C. rose blossom, ange ta et ( te hnEs hc ol eue sntinsb microorganisms. by nutrients as used compounds be could some which in EOs, hydrosols than other the enrich previously may process distillation 2010 been the Iyer, & has More, Prabhudharwadkar, evaporation 2012; Elistratov, during ( droplets described water 1) = entrain- of (n The 0.31% products. and distillation ment 3), the = in (n found 0.4% were compounds ± these 3.5 of 3), = (n 0.4% ± 0.74 Respectively n urs,ec tacnetaino 0gL con glycin, g/L, 20 extract, of concentration yeast a of at each compounds sucrose, and non-volatile pure of The distillations Laboratory solutions mg/L. steam. of by 2 entrainment and their suggests distilled mg/L freshly hydrosols in compounds 0.7 non-volatile of amounts respectively low of detection, presence of threshold the In hydrosol. than blossom lower concentrations were orange acid distilled amino and freshly organic hydrosols, a these hydrosols, in blossom C. mg/L rose 0.03 distilled ± freshly orange 0.38 two of in and mg/L of 0.02 ± ± 0.21 0.25 sd) and distillate deviation, mg/L standard 0.01 ± laboratory (mean of concentrations a at detected in except ( mg/L, 702 al 3 Table .aurantium C. yrslpswr opie ewe H4adp n were and 7 pH and 4 pH between comprised were pHs Hydrosol l ,2011 ., nuscri oacentifolia (Rosa C. rose and ), NT NT NT NT NT NT NT NT NT NT NT NT abhdae (mg/L) Carbohydrates .8±0.03 ± 0.38 NT NT NT NT NT NT NT NT 0.01 ± 0.21 NT NT 0.02 ± 0.25 .Tedymte otnswr opie ewe 5and 95 between comprised were contents matter dry The ). .Hwvrtecnetain fteohrcmonsi or- in compounds other the of concentrations the However ). pt publis oady&vnRh,20;Nkrao,Msua & Misyura, Nakoryakov, 2001; Rohr, von & Cosandey #9, al 3 Table – 586 hed hed ﬂ hr tws40m/.Croyrtswere Carbohydrates mg/L. 4.0 was it where ) in wrhdoo ( hydrosol ower yrsl rmdvreoiisadsoe ndifferent in stored and origins diverse from hydrosols ) :

ﬁ in oalwmcoilgclstability, microbiological allow to cient ﬂ wrhdooswr . to 1.4 were hydrosols ower

3.8 5.5 7 ND 3.9 4.5 4.5 4.6 5.5 6.3 ND b 6.4 5.9 6.1 4.2 7.1 7 b b 4.8 5.8 b log counts Microbiological 4.5 5.8 0.7 0.7 0.7 0.7 10 CFU/mL lsye l,2009 al., et Ulusoy fi mdti hypothesis. this rmed .Consequently ). 5.7 5.5 7 6 ND 6.1 4.8 4.8 6 6.5 5.1 b 7.1 5.9 6.1 ND 7.3 6.6 b b 5.4 5.8 b log 4.7 5.8 4.00 4.00 4.00 4.00 ). 10 cell/mL N fl 1000- ower 581 Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript ntetors .hdoos aia atra ouain aot6to 6 log (about populations bacterial 7 maximal hydrosols, C. rose two the In yrslwslwrta . log 0.7 the in than population lower microbial the was distillation, the hydrosol of end the At tillation. of kg 300 Approximately #,#7 2. n #21.2, ( line and distillation the #21.1 of end #17, the (#9, at container sterile a in collected drosols ±.)frbcei n . log 3.9 and bacteria for (±0.3) hrfr civda es log 4 a least at achieved therefore w ifrn atrs ihrbceilcut htpaeuda to 6 at plateaued that counts showed °C bacterial 7log 5 either ambient at patterns: at stored different hydrosol days this two 10 of samples within two reached The temperature. were populations bacterial and mal ( three- #18.2 a period and over #8.2, storage followed #18.1 #7.2, were month #8.1, populations (samples bacterial #7.1, in temperature (samples Changes °C #22.2). ambient 5 at at or stored #22.1) were #22) and (#18 582 rdcin he irbooia onshv enpromda ahsmln aeadec a ersne yadt )Hdoos#. n 72;B yrsl 81ad#8.2; and #8.1 hydrosols B) #7.2.; and #7.1 Hydrosols A) dot. a by represented was ( #22.2 each and and #22.1 hydrosols date D) sampling #18.2; each and at #18.1 hydrosols performed C) been have counts microbiological Three production. 2. Fig. time storage with changes and counts Microbiological 3.2. i n log 3 and ria eraei h irba ons hc eelwrta h threshold the (i.e., than enumeration lower of were which counts, microbial the in decrease pronounced a or samples, studied other the in observed counts maximal min eprtr,adin and temperature, ambient increbtenCUcut n elcut a .4 hc was which 1.04, was counts cell signi and not regres- counts linear CFU the of between slope curve The counts. sion cell the to close were suffi counts Further- not CFU was concentration. storage EO cold or a pH, more, production, of year CFU/mL log10 manufacturer, 7 and 4 between ( were counts bacterial total shelf-life, ( °C 5 at S)soe htms el eentval.A osqec the consequence As viable. not were cells most Eugene, Probes, that (Molecular showed assay counts, CFU USA) kit than viability higher markedly BacLight counts cell Live/Dead® with a few the of one °C, 5 Journal homepage Food Research International b Ve al 3 Table . log 0.7 w rnebosmhdoo ape # n 8 n w oeC. rose two and #8) and (#7 samples hydrosol blossom orange Two irbooia onsi rs oeC. rose fresh in counts Microbiological damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa 10 hne nbceilcut t5° ( °C 5 at counts bacterial in Changes si 10 on définitive du m définitive on F/Loe h xmndsoaepro ( period storage examined the over CFU/mL i.2 Fig. F/L eerahdwti e aswe trdat stored when days few a within reached were CFU/mL) .Hg o o)bceilcut eentrltdwt any with related not were counts bacterial low) (or High ). Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de fi 10 atydfeetfo (P 1 from different cantly F/L uigatremnhsoaeperiod. storage three-month a during CFU/mL) 10 n ) o yrsl nlzda aibetmsduring times variable at analyzed hydrosols For D). and C , F/L(05 euto o essadmls h hy- The molds. and yeasts for reduction (±0.5) CFU/mL Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 b 1log

i.2 Fig. : http://www. ﬂ 10 wr n 5 fwtraemxdfrdis- for mixed are water of L 750 and owers anu F/L fe 0dy ( days 80 after CFU/mL)

al 3 Table ﬁ

log10 CFU/mL log10 CFU/mL maxi- hydrosols, blossom orange In ). te ast w otswe stored when months two to days fteen 10 scr 0 2 4 6 8 0 2 4 6 8 10 ▲ 10 F/ ±.)fryat n molds. and yeasts for (±0.5) CFU/g in oisr tblt.Ms fthe of Most stability. insure to cient n 0° ( °C 20 and ) 020406080 020406080 F/L(03 euto o bacte- for reduction (±0.3) CFU/mL A it C

F/L h itlainprocess distillation The CFU/mL. hwdcut eann low remaining counts showed ) p N (201 .5.I ape#. trdat stored #8.1 sample In 0.05). ub elsevier.com/locate Storage time(days) Storage time(days) ﬂ lié Comment citer cedocument: 5 wr ee49log 4.9 were owers ○ ), e noag lso ( blossom orange in )

Vol. s / s dan .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. al 1 Table i.2 Fig.

76 i.2 Fig. Fi - 576 , p. ). ,A),i.e.,atthe na ,B)(

l ver al 3 Table 10 irsaurantiumCitrus CFU/g 586, si / foodres on of the ma on of ). DOI )(A,B)androseC.( ( 2005 al., et Park 2007; ( contaminants vironmental en- as known species, Gram-negative were repre- them of 58 isolated All strains. we sentative way, this In isolates. among clones isolated permitted 13 strains eliminating been 40 have on strains (M13-PCR) typing bacteria molecular 71 A hydrosols. total, from In agar. YGC or DPCA onto identi Bacteria 3.3. likely state. culturable were hydrosols a in in present micro-organisms viable of part major ftesrisi ormjrbace:a branches: major four in strains the of cepacia a sltdfo yrsl fdfeettpsaddfeetaesand ( areas different production and of types year different of hydrosols from example isolated For was hydrosols. sam- some to in associated 4.0 highly as seem low They (as hydrosols ples). of pHs of range the at acido-tolerant growth and as described are them ( of Many hydrosols. in multiply ( Archive Nucleotide www.ebi.ac.uk/ena) European the in and LN831188 respectively, numbers accession LN831189, the under available whole are The species new S1). Fig. data mentary pce eogn to belonging species apeo rnebosmhdoo a been has hydrosol isolates blossom orange water of in sample a represented largely are ( and low require growth, species These for isolates. hydrosol nutrients 58 the of out 39 represent trg otiesi h pnar n nyincpatcsaeless are practices unhygienic and air, open units, the small in in hydrosol containers production of storage but Conditions variable, hydrosols. very studied are nor the production yeast in Neither detected was data). mold (unpublished orange containers contaminated in hydrosol observed blossom sediment brown to yellow the with 2001 ( Hiraishi, whitish-brown & or Hamana, yellow Takeuchi, are colonies Its water. tilled : : lmr ta. 96 aleee l,2009 al., et Vanlaere 1996; al., et Elomari al 4 Table tpie ta. 2014 al., et Stopnisek

log10 CFU/mL log10 CFU/mL 10.1016/j.foodres.2015.07.014 nybcei eedtce ntetse yrsl,ete spread either hydrosols, tested the in detected were bacteria Only 0 2 4 6 8 0 2 4 6 8 204060800 020406080 nuscri ope rnh n w rnhsognzdaon unde around organized branches two and branch, complex B D .Pyoeybsdon based Phylogeny ). Storage time(days) Storage time(days) pt publis oacentifolia Rosa ﬁ – ainadphylogeny and cation 586 ihydsatbceilseiswr beto able were species bacterial distant Highly . al 4 Table Rhodospirillaceae ,adblne oalmtdnme fgenera of number limited a to belonged and ), n r hrfr ahraatdt survival to adapted rather therefore are and ) hed hed C )i yrsl rmdfeetoiisado erof year and/or origins different from hydrosols in D) (C, ) lmr ta. 96 o,Zag u Xie, & Su, Zhang, Lou, 1996; al., et Elomari ). in Pseudomonas :

rrs rrs n hscudb nagreement in be could this and ) ee hwda arrangement an showed genes

ee fteetopresumed two these of genes and Pseudomonas ). Acetobacteraceae and .capsulatum N. fi s sltdfo dis- from isolated rst Burkholderia p rnh a branch, sp. efo,1962; Leifson, sltdin isolated .veronii P. (Supple- fi species ln of lling http:// fi ned Bu. Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve httevltl pro volatile the that compounds volatile on bacteria of Impact 3.4. identi bacteria environmental the ( uncommon than Identi 4 Table damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food f e d c b a oacentifolia Rosa oadamascena Rosa irsaurantium Citrus yrslIdenti Hydrosol r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si rsmdnwsei acsinnme N319;N:ntdetermined. not ND: LN831189); number (accession specie new Presumed aeas Same rrs rsmdnwsei acsinnme LN831188). number (accession specie new Presumed oag blossom) (orange h nlsso Ocmoiino yrsl uigseflf showed life shelf during hydrosols of composition EO of analyses The ubro lnlioae one u ymlclrtpn (M13-PCR). typing molecular by out pointed isolates clonal of Number pce ujce opeoyi et o identi for tests phenotypic to subjected Species on définitive du m définitive on fi aino yrslbacteria. hydrosol of cation eesqec identity. sequence gene Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de al 1 Table rs C.) (rose Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 rs D.) (rose . at 2015 Watt, fi

emycag ihtm.Freape oeC. rose example, For time. with change may le : http://www. suooa veronii Pseudomonas protegens Pseudomonas protegens Pseudomonas protegens Pseudomonas Gluconacetobacter suooa veronii Pseudomonas ukodraambifaria Burkholderia arboris Burkholderia efi acidovorans Delftia lividum Janthinobacterium baderi Sphingobium wautersii Acidovorax amazonense Azospirillum diffusa Burkholderia lata Burkholderia lata Burkholderia lata Burkholderia lata Burkholderia lata Burkholderia panacis Pseudomonas panacis Pseudomonas panacis Pseudomonas veronii Pseudomonas veronii Pseudomonas veronii Pseudomonas veronii Pseudomonas veronii Pseudomonas Achromobacter methanolica Acidomonas veronii Pseudomonas veronii Pseudomonas suooa veronii Pseudomonas cloacae Enterobacter capsulatum Novosphingobium methanolica Acidomonas methanolica Acidomonas methanolica Acidomonas amazonense Azospirillum amazonense Azospirillum amazonense Azospirillum amazonense Azospirillum amazonense Azospirillum amazonense Azospirillum lata Burkholderia lata Burkholderia lata Burkholderia lata Burkholderia vietnamiensis Burkholderia vietnamiensis Burkholderia vietnamiensis Burkholderia vietnamiensis Burkholderia vietnamiensis Burkholderia cedrina Pseudomonas panacis Pseudomonas panacis Pseudomonas panacis Pseudomonas veronii Pseudomonas veronii Pseudomonas anu

ﬁ scr n r h as fcnaiainby contamination of cause the are and ) ainIett (%) Identity cation it ﬁ

p di hswork. this in ed (201 ub sp. elsevier.com/locate p 0 1 100 sp. lié Comment citer cedocument: 5 ﬁ ), cation. e

Vol. s / s dan .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C.

76 0 1 99 99 100 99 0 1 100 91IA_FO_02 1 99 nNCBI on 91I_O0 DC21 Tunisia 2013 C ND 3 IA_FO_08 IA_FO_07 1 1 99 99 0 2 IA_RC_30 99 100 98 IA_RC_34 2 99 IA_RC_13 96 99 99 IA_RC_32 1 99 2 99 99 IA_RC_01 99 1 99 99 99 2 99 99 99 99 99 97 99 99 51IA_FO_17 1 IA_FO_18 99 99 99 95 1 96 97 95 95 95 96 95 95 1 99 99 99 100 99 99 99 99 99 99 99 99 99 99 99 Fi - 576 , p. na

l a ver 586, 2 1 2 isolates of Number 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 3 1 1 1 1 1 1 1 si / foodres on of the ma on of DOI b : : AR_41 ND 20 5 19 18.2 IA_RC_11 18.1 IA_RC_04 IA_RC_10 IA_RC_09 AR_31 ND ND 14 14 IA_RD_03 IA_RD_02 strain Representative AR_82 ND 5 ND ND 20 18.1 20 20 IA_RC_08 20 IA_RC_14 IA_RC_07 18.2 IA_RC_06 IA_RC_03 IA_RC_16 IA_RC_39 IA_RC_24 IA_RC_18 IA_RC_17 IA_RC_12 IA_RC_40 IA_RC_22 IA_RC_05 IA_RD_04 IA_RD_01 IA_RD_07 AF_63ND 20 3 8.2 IA_RD_06 IA_FO_06 IA_FO_33 IA_FO_11 IA_FO_13 IA_FO_25 IA_FO_22 IA_FO_10 IA_FO_12 IA_FO_05 IA_FO_34 IA_FO_26 IA_FO_23 ND IA_FO_15 IA_FO_31 IA_FO_29 2 IA_FO_28 IA_FO_21 IA_FO_09 IA_FO_01 IA_FO_20 IA_FO_16 IA_FO_19 IA_FO_04 IA_FO_03 10.1016/j.foodres.2015.07.014 lso yrsl#. trda 0° oiatycnaiae by contaminated dominantly °C 20 vietnamiensis at Bu. stored #8.2 hydrosol orangeblossom the in observed were changes pronounced contrast In storage. of pro volatile same the kept temperature, ( (#17) contaminated not lividum Janthinobacterium which in hydrosols nuscri f f f e e e e e e d d d d d d d d d d d d d d d d e d d d d d d d d d d d d d d f d d d pt publis c Sample# 0Ambient 10 9ND 20 19 20 5 22.2 20 22.2 20 22.1 20 18.2 5 18.2 5 22.2 20 18.1 20 18.1 5 ND 22.2 ND 22.2 22.1 5 19 19 18.1 ND ND ND ND 14 16 15 15 Ambient ND 20 4 5 ND 1 Ambient 7.2 ND 7.1 ND 5 ND 4 1 0 3 20 5 20 7.2 5 7.1 5 6.2 20 8.1 5 ND 8.1 8.2 7.1 5 3 20 5 ND 6.1 ND 6.2 ND 6.1 2 2 2 ND 5 and – 586 Pseudomonas hed hed .capsulatum N. eprtr (°C) temperature Storage in al 3 Table eetems omnioae #8,or (#18), isolates common most the were :

sp., ,soe t5° swl sambient as well as °C 5 at stored ),

nwihlnlo n geraniol and linalool which in , u lata Bu. B B B I I auatrrYa Production Year Manufacturer D B B B B B B B B B B B B B B B B B B B B I K J J C E D A D D A D A D C D D B E E E D C B B B B B B B A ﬁ edrn tlatsxmonths six least at during le , ukodradiffusa Burkholderia 02France 2012 France 2013 France 2013 03Morocco 2013 Morocco 2013 04Tunisia 2014 02France 2012 France 2013 France 2012 France 2012 France 2012 France 2013 France 2014 France 2014 France 2014 France 2013 France 2013 France 2014 France 2013 France 2013 France 2014 France 2014 France 2014 France 2012 France 2012 France 2013 Morocco 2013 Bulgaria 2013 Turkey 2013 Turkey 2013 03Tunisia 2013 Tunisia 2014 Tunisia 2013 Tunisia 2013 Tunisia 2014 Tunisia 2014 Tunisia 2013 Tunisia 2013 Tunisia 2013 Tunisia 2012 Tunisia 2013 Tunisia 2014 Tunisia 2014 France 2014 Tunisia 2014 Tunisia 2014 Tunisia 2014 Tunisia 2014 Tunisia 2013 France 2013 France 2014 France 2014 France 2014 France 2013 France 2013 France 2013 03Tunisia 2013 country and , 583 Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript vietnamiensis 584 h trl oto n h yrsliouae ihec tanat strain each with inoculated hydrosol the and control sterile the eetd ocnrto oe hntedtcintrsodlmt(bu gL.Err assronigma ausrpeets n=3.* * niae signi a indicates (*) ** 3). = (n sd represent values mean surrounding bars Errors mg/L). 1 (about limit threshold detection the than concentration lower detected, with inoculated hydrosol stripes: horizontal i.3. Fig. ( hydrosols mg/L. 128 6-methyl- concomitantly to and mg/L mg/L) 1.3 2.2 from to increased concentration to 5-hepten-2-one mg/L mg/L 61 343 from and from mg/L 146 (respectively decreased markedly concentrations 01 amla&Hre,2014 Harder, & Marmulla ( 2001; pathways different follows micro-organisms by noids metabolize was which to acetate phenylethyl (iii) and hydro- C. rose sol, sterile the in able acid citronellic was into citronellol convert CFU/mL, to (ii) (±0.04) hydrosols, both 6-methyl-5-hepten-2-one 6.6 in into geraniol convert and to (i) CFU/mL (±0.1) 7.0 respectively capsulatum N. between (difference signi samples inoculated hydrosol and control blossom sterile orange 2- the and synthesize convert in acid to to geranic phenylethanol (ii), respectively and and into hydrosol acetate geraniol phenylethyl C. and rose metabolize geraniol the in to acid that geranic (i) showed and able citronellol CFU/mL, was (±0.2) 7.0 species and this CFU/mL (±0.1) 8.0 spectively abnsuc ( source carbon 2001 ( methyl-5-hepten-2-one and plasmid, a to thanks control sterile between (difference signi samples hydrosol inoculated and blossom orange the in Journal homepage Food Research International Ve nlsso h Ocmoiino oeC n rneblossom orange and C. rose of composition EO the of Analysis damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa matof Impact .Moreover ). si on définitive du m définitive on Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de i.3 Fig. u vietnamiensisBu. sri AF_8,at IA_FO_28), (strain sri AF_3,at IA_FO_33), (strain adneg oe 96 adneg Wright, & Vandenbergh 1986; Cole, & Vandenbergh Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 .putida P. ,atriouainwt tanof strain a with inoculation after ),

: http://www. eiilu digitatum Penicillium adneg,18;Wle a e Werf, der van & Wolken 1989; Vandenbergh, and a s eail(rctoell stesole the as citronellol) (or geraniol use can anu ﬁ

.capsulatum N. atat cant α

Concentration (mg/L) Concentration (mg/L) ﬁ scr triel ehlatrnlt and anthranilate methyl -terpineol, 100 100 al ovre no2-phenylethanol into converted nally .Frexample For ). 10 10 1 1 ukodravietnamiensisBurkholderia ﬁ it

a atracnetain fre- of concentrations bacteria nal P p (201 ﬁ b a atra ocnrtosof concentrations bacterial nal ub .1.Mtblzto fterpe- of Metabolization 0.01). naoai opud in compounds aromatic on Novosphingobium capsulatum Burkholderia vietnamiensis sterile elsevier.com/locate lié Comment citer cedocument: 5 ﬁ NDND ** ), atat cant e

Vol. ovr eailit 6- into geraniol convert s / s dan ** .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. suooa putida Pseudomonas

P 76 P b * b .1.Similarly, 0.01). Fi - 576 , p. .1( 0.01 Demyttenaere, ** tanI_O2;vria tie,hdoo ncltdwith inoculated hydrosol stripes, vertical IA_FO_28; strain na Burkholderia

** P l b ver irsaurantium Citrus .5 (Mann 0.05) ** 586, si / ND foodres on of the ma on of ND , DOI – hte test). Whitney ogtr trg tlwtmeaue tblt a ob obtained rules be sanitary to with has comply must stability which temperature, preservatives, low chemical with at storage term long and/or line tion 2013 atra otmnnscudb lmntdb atuiain(no pro pasteurization aromatic the by identi eliminated identi bacteria be the spore-forming All could contamination. such contaminants for bacterial marker chemical a as used speci was impact drosols an have pro volatile operations on process during hydrosols contaminating ano w AHmlcls( molecules NADH two a with of dehydrogenase, geranial gain and geraniol a using acid geranic into ol 1983 eu irboaal opoieaet eaieyhg ubr u to (up numbers high relatively 10 to proliferate to able microbiota neous Conclusion 4. vietnamiensis strains Bu. those in activities enzymatic such identify to dehydroge- citronellal ( the nase and dehydrogenase geraniol reactions the enzymatic successive involving by acid citronellic into citronellol verts A and (A) : : ** ** 10.1016/j.foodres.2015.07.014 6 yrsl foag lso,rs . n oeDhrosaheteroge- a harbors D rose and C., rose blossom, orange of Hydrosols – 10 .Smlrytebacterium the Similarly ). .I h bec fa spi akgn tteedo h distilla- the of end the at packaging aseptic an of absence the In ). DND ND ND öse-rmee l,2006 al., et Förster-Fromme oacentifolia Rosa nuscri 7 F/L ept h rsneo O.Sm ftestrains the of Some EOs. of presence the despite CFU/mL) ** pt publis fi ** e h rdcino -ehl5hpe--n nhy- in 6-methyl-5-hepten-2-one of production The le. fi fi ersnaieo h yrslmicrobiota. hydrosol the of representative B yrsl fe 0dy ficbto.Fl ry trl hydrosol; sterile gray: Full incubation. of days 10 after hydrosols (B) adr uesn aa eimsCna,&Kineci, & Secilmis-Canbay, Kara, Kuleasan, (Baydar, le tain n eas fterltv inef relative the of because and ltration, fi – 586 otepeec of presence the to c ** hed hed * oopigbu capsulatumNovosphingobium ND in üdk ta. 2012 al., et Lüddeke atlailadefragransCastellaniella fi : d,btwt osbeatrto of alteration possible a with but ed),

ND .Frhrrsac ol enecessary be would research Further ). A B ** **

ND .capsulatum N. fi ). tanI_O3.N:not ND: IA_FO_33. strain atdfeec between difference cant .capsulatum N. .aeruginosa P. ovrsgerani- converts n ol be could and fi inyof ciency con- fi and ed Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Journal homepage Food Research International Ve ut .(04.Esnilol:teratbceilpoete n oeta applications potential and properties antibacterial their oils: Essential (2004). S. Burt, (2012). F. Zagrouba, & M., Romdhane, F., Mathieu, A., Lebrihi, J., Bouajila, H., A. Ammar, hraools .G,Gaui,E . knai,P . aotuin .A,&Nca,G J. G. Nychas, & A., S. Haroutounian, N., P. Skandamis, D., E. Giaouris, G., N. Chorianopoulos, lTri .I (2007). I. A. Al-Turki, adr . uesn . aa . eimsCna,H,&Knc,S 21) h fet of effects The (2013). S. Kineci, & H., Secilmis-Canbay, N., Kara, H., Kuleasan, H., Baydar, (2005). R. Ur, Shoaib & M., Khan, Aslam (2012). ANSM oetn,S,Tbrs,C .G,Psn,B,Sta . aca . rei .(99.In-vitro (1999). E. Arzedi, & V., Mascia, M., Satta, B., Pisano, G., I. C. Tuberoso, S., Cosentino, i-uzo,A,Cert . sia . oa,S,Rt,C,&Pgn .(01.Teantimicro- The (2011). R. Pagan, & C., Rota, S., Loran, L., Espina, L., Cherrat, A., Ait-Ouazzou, gra,S . ut,A,Kph,B . aeha,Tap,R,&Sr,O .(05.Chemical (2005). P. O. Suri, & R., Thappa, Baleshwar, K., B. Kapahi, A., Gupta, G., S. Agarwal, (INRA, Berge Odile to due Technologie la the de from grant a received has Acknowledgments the of quality sensory products. delicate and chemical physical, the keep also and Cosandey,J.O.,&vonRohr,P.R.(2001).Entrainmentofsolubleandnonsolubletracersfrom Gavsdo hmclaayi n nepeaino hmcldata. chemical identi bacterial of design interpretation to contributed and manuscript. MHG the analysis of chemical versions on initial advised the CG wrote and data the interpreted Contributors mi- in assistance for analysis. Malléa crobiological Sabine and Oriol Stéphanie to and cussions advn ( Baldovini References doi.org/10.1016/j.foodres.2015.07.014 data Supplementary A. Appendix CL. author of salary the covered their from partly salaries ANRT received institutions. authors home All INRA. and SAS Vieille Albert tween sources funding the of Role manuscript. the on commented of authors versions initial All the paper. revised the and the project charac- the of supervised and collection process FC hydrosol terization. the the about coordinated advised CC and samples work. analyzed experimental the of de- and sign conception the to contribution substantial a made CR proaches. damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si aiies [Article] sanitizers. of hydrosol and essen- oil of tial effect bactericidal bacteria: pathogenic and spoilage technological, of posed nfoods in PJBS of activities antioxidant and aurantium antimicrobial vitro in and composition Chemical Environment and Agriculture .(08.Dsnetn etaantmnclueadmxdcluebio mixed-culture and monoculture against test Disinfectant (2008). E. http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.022 151 alchdoosagainst hydrosols garlic n cn opsto frs water. rose of composition scent and spoilage microbial on preservatives chemical and radiation ultraviolet pasteurization, from (Retrieved ed.) (11e française niirba ciiyadceia opsto fSriinTyu seta oils. essential Thymus Sardinian of composition chemical and activity antimicrobial org/10.1016/S0029-5493(01)00354-5 ilatvt fhdohbcesnilolcnttet cigaoeo ncombined in or 12 alone preservation. acting food constituents of oil processes essential hydrophobic of activity bial http://dx.doi.org/10.1080/10412905.2005.9698897 do volatiles. water rose of composition species. oln ae surface. water boiling a hswr saprilful partial a is work This Lcridotms fteeprmna ok nlzdand analyzed work, experimental the of most out carried CL upeetr aat hsatcecnb on nieat online found be can article this to data Supplementary be- 21000467 number contract research a by funded was research The on définitive du m définitive on 3,320 (3), i. org/10.1111/j.1365-2672.2007.03694.x – Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de , 160. 15 2) 1034 (21), nentoa ora fArclueadBiology and Agriculture of Journal International — nvriéd Nice de Université http://dx.doi.org/10.1080/0972060x.2013.794043 – l. 329. review. a axdsile ééae teu rmtse végétales aromatisées eaux et végétales distillées Eaux fl wr seta i Nrl oil). (Neroli oil essential owers Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 http://dx.doi.org/10.1016/j.ifset.2011.04.004 ai,Fac,udrcnrc 0200.Tak are Thanks 2012/0306. contract under France, Paris, , niatra feto hm,ppemn,sg,bakppe and pepper black sage, peppermint, thyme, of effect Antibacterial – 1040. ora fApidMicrobiology Applied of Journal nentoa ora fFo Microbiology Food of Journal International

ula niern n Design and Engineering Nuclear aueathymbra Satureja : http://www. ailssubtilis Bacillus http://dx.doi.org/10.3923/pjbs.2012.1034.1040 anu , 5

ﬁ 2,92 (2), ohaAtpls o epu scienti helpful for Antipolis) Sophia leto éieLbdesPDtei,who thesis, PhD Labadie's Cécile of llment eted ehrh PACA Recherche de Centre scr noaieFo cec mrigTechnologies Emerging & Science Food Innovative soito ainl el ehrh et Recherche la de Nationale Association www.ansm.sante.fr ora fEsnilOlResearch Oil Essential of Journal . – xrcinadaayi fesnilolo Rosa of oil essential of analysis and Extraction it 94.

ora fEsnilOlBaigPlants Oil-Bearing Essential of Journal p (201 and n oprsnwt tnadacid standard with comparison and ub . . elsevier.com/locate lié amnlaenteritidis Salmonella Comment citer cedocument: 5 aitnJunlo ilgclSciences: Biological of Journal Pakistan ), e ﬁ

. Vol. ain n hlgn ap- phylogeny and cations . s / s dan , 104 , , 7 atacs nMrh2015). March on access last , 208 .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. 6,973 (6), (6),

76 . 1,87 (1), . 1586 Fi - 576 , p. – na 974. , – n Nicolas and )

– , 94 . 97. 1596. 17 l ora fFood, of Journal 3,223 (3), ver Pharmacopée 3,265 (3), http://dx.doi. http://dx. . fi 586, http://dx. m com- lms si / fi foodres , on of the ma on of cdis- 16 – – Citrus – base 253. 267. (2), DOI , : : 10.1016/j.foodres.2015.07.014 ak .-. e,H . i . i,Y,Be .S,Co,J E,e l (2005). al. et -E., J. Choi, S., K. Bae, Y., Kim, H., Yi, B., H. Lee, -D., Y. Park, ihr . hlis .(08.Ptnilatmcoilue fesnilol nfo:i cit- is food: in oils essential of uses antimicrobial Potential (2008). C. Phillips, & K., Fisher, (2014). A. Casale, & C., André, X., Fernandez, eno,V,Cabu,J,Rsel . ae,P 20) Quanti (2005). P. Baret, & D., Russell, J., Chahboun, V., Jeannot, efo,E 16) h bacterial The (1962). E. Leifson, rbuhrakr .M,Mr,R . yr .N 21) xeietlsuyo liquid of study Experimental (2010). N. K. Iyer, & Z., R. More, M., D. Prabhudharwadkar, aoykv .E,Msua .Y,&Eitao,S .(02.Tebhvo fwtrdroplets water of behavior The (2012). L. S. Elistratov, & Y., S. Misyura, E., V. Nakoryakov, öse-rme . öcl,B,Mc,C. Mack, B., Höschle, K., Förster-Fromme, (2001). R. C. J. Demyttenaere, (2014). Europe of Council lmr,M,Crlr . ot,B,Gli,M,Iad . elr,H 19) N relatedness DNA (1996). H. Leclerc, & D., Izard, M., Gillis, B., Hoste, L., Coroler, M., Elomari, vnv,E . oskv,N . aae . aah,K,Klnvky,N . yek,A M., A. Lysenko, I., N. Kalinovskaya, K., Hayashi, T., Sawabe, M., N. Gorshkova, P., E. Ivanova, unbeir,M . eg,O,Nrad . ors . aln . gynTe .(2001). C. Nguyen-The, & F., Carlin, C., Morris, P., Normand, O., Berge, H., M. Guinebretiere, tpie,N,Bdnasn . ry . irr . br,L,&Wisof .(04.Genus- (2014). L. Weisskopf, & L., Eberl, N., Fierer, B., Frey, N., Bodenhausen, N., Stopnisek, (1958). K. Goodner, & Jr., L., H. Smith, toxigenic and non-toxigenic of Inactivation (2013). F. Tornuk, & I., Ozturk, O., Sagdic, amla . adr .(04.Mcoilmntreetransformations monoterpene Microbial (2014). J. Harder, & R., Marmulla, uos . ils .A,Hmlo,J . ees .A,&Sih .(96.Colorimetric (1956). F. Smith, & A., P. Rebers, K., J. Hamilton, A., K. Gilles, M., Dubois, Fr u,S W,Ln .-. eg .-. hn .-.(06.Ef (2006). -J. M. Chen, & -M., Y. Weng, -C., Y. Lin, -W., S. Sun, o,M m,Zag .-. u . i,G l 20) eooasof Genomovars (2007). -l. G. Xie, & T., Su, -x., L. Zhang, -m., M. Lou, unbeir,M . gynTe .(03.Sucsof Sources (2003). C. Nguyen-The, & H., M. Guinebretiere, aeci . aaa . iasi .(01.Pooa ftegenus the of Proposal (2001). A. Hiraishi, & K., Hamana, M., Takeuchi, L abuia,M,Saaior . aina .(2014). K. Javidnia, & S., Shahabipour, M., Mahboubifar, üdde utos 2113 2,94– 15(2), nentoa ora fSseai n vltoayMicrobiology Evolutionary and Systematic of Journal International panacis 6609 u h answer? the rus tions. 1138 veronii Pseudomonas ( water blossom orange natural from extracted oil essential the of composition chemical reservoir. genera aroe nasprtrdrum. separator a in carryover http://dx.doi.org/10.1099/ijs.0.63592-0 ntehae surface. heated the on org/10.1016/j.tifs.2007.11.006 (Ed.), Elsevier (9783769261165). ed.) (8th Pharmacopoeia European 765X.1999.00605.x among ta.(2002). al. et 1574-6941.2003.tb01060.x Identi 1111 soil of populations. distribution biogeographical the for accounts tolerance acid wide methods. plate sanitizers. food natural as spices Lamiaceae of Control hydrosols of utilization bers: coli Escherichia http://dx.doi.org/10.1016/j.nucengdes.2009.09.018 rnir nMicrobiology in Frontiers ehdfrdtriaino uasadrltdsubstances. related and sugars of determination for method Elsevier. 00853-06. in Microbiology Environmental leucine/isovalerate and terpenes Identi (2006). 350 28(3), Microbiology Applied in Letters h ai fpyoeei n hmtxnmcanalyses. chemotaxonomic and phylogenetic of basis the org/10.1099/0096266x-12-4-161 Taxonomy and Nomenclature Bacteriological of Bulletin 6,1149 21(6), gentamicin. for assay colorimetric atuie uciiprepoesn ie ifrnitdb w PCR-based two by differentiated line, processing purée methods. zucchini pasteurized a purees. vegetable pasteurized other in 1128/aem.67.10.4520-4530.2001 Microbiology found Environmental those and with Applied comparison and tures 6 defragrans Castellaniella by degradation monoterpene anaerobic in induced dehydrogenases geranial and niol stricto quanti acid Analysis amino for method ninhydrin ope rmrc hzshr n lnci China. in clinic and http://dx.doi.org/10.1016/S1672-6308(07)60032-8 rhizosphere rice from complex tdcmoet nIainrs water. rose Iranian in components ated http://dx.doi.org/10.1128/aem.07226-11 irsaurantium Citrus nuscri 1) 7. (11), ke , . ord,S,PrzLrno .E,&Fuo,G 20) aiae quantitative validated A (2000). G. Frutos, & E., M. Perez-Lorenzo, S., Torrado, P., . Wül F., , – – – / ai:Vuibert. Paris: fi 1462-2920.12211 2120. 6617. 1144. n he e genera, new three and , aino atrai atuie uciipre trda ifrn tempera- different at stored purees zucchini pasteurized in bacteria of cation Corynebacterium Pseudomonas , 30 p o. sltdfo h ufc frsyroso oenginseng. Korean of roots rusty of surface the from isolated nov., sp. 19 pt publis ESMcoilg Ecology Microbiology FEMS 97. nentoa ora fSseai n vltoayMicrobiology Evolutionary and Systematic of Journal International – 1,7 (1), (2 356. – fi http://dx.doi.org/10.1099/ijs.0.02197-0 http://dx.doi.org/10.1016/j.ijheatmasstransfer.2012.06.069 http://dx.doi.org/10.1099/00207713-46-4-1138 1159. niomna Microbiology Environmental – g . ik,M,Gre,F,Wbr . Dik J., Weber, F., Germer, M., Timke, A., ng, suooa extremorientalis Pseudomonas http://dx.doi.org/10.1016/j.ijat.2005.03.012 tde nNtrlPout Chemistry Products Natural in Studies ) 112 3), fi ora fBacteriology of Journal 17H ncltdo iial rcse oaosadcucum- and tomatoes processed minimally on inoculated O157:H7 – aino ee n rtisncsayfrctbls facyclic of catabolism for necessary proteins and genes of cation http://dx.doi.org/10.1021/ac60111a017 rnsi odSine&Technology & Science Food in Trends 14. .ssp. L. . http://dx.doi.org/10.1016/s0731-7085(99)00192-2 – 586 http://dx.doi.org/10.1016/j.foodcont.2012.07.010 p nov. sp. tan sltdfo aua iea aesadpooa of proposal and waters mineral natural from isolated strains – hed hed uoendrcoaefrteqaiyo eiie healthcare & medicines of quality the for directorate European 117. . . nentoa ora fHa n asTransfer Mass and Heat of Journal International , , ple n niomna Microbiology Environmental and Applied aurantium 5 , Flavobacterium . . itasomto ftreod ymcoraim.In microorganisms. by terpenoids of Biotransformation http://dx.doi.org/10.3389/fmicb.2014.00346 fl http://dx.doi.org/10.1016/j.jfca.2005.04.006 in r fdsildadsoe ae.II e pce fthe of species New III. water. stored and distilled of ora , . nentoa ora fSseai Bacteriology Systematic of Journal International 7,4819 72(7), Sphingobium , : 29 . .

ula niern n Design and Engineering Nuclear eeto fbceilgltnssb gelatin by gelatinases bacterial of Detection ora fPamcuia n imdclAnalysis Biomedical and Pharmaceutical of Journal 2,130 (2), .[article] ). , , 76 43 . ot . rbutr . edosk D. Jendrossek, & W., Armbruster, M., Bott, , . , nentoa ora fCeTc Research ChemTech of Journal International 6,662 (6), yrlt teaux et Hydrolats 2,207 (2),

, suooa aeruginosa Pseudomonas 67 Ppirillum fi – 16 – , cation. p o. sltdfo rnigwater drinking a from isolated nov., sp. 4828. 1) 4520 (10), 135. Novosphingobium 6,1503 (6), nentoa ora fAromatherapy of Journal International – – http://dx.doi.org/10.1046/j.1472- 665. vlaino h aubeoxygen- valuable the of Evaluation . . 215. http://dx.doi.org/10.1128/aem. . ora fFo opsto and Composition Food of Journal , and ailscereus Bacillus , o.25 vol. , ieScience Rice 19 12 – – fi . fi http://dx.doi.org/10.1111/j. 3,156 (3), . Pseudomonas 1512. 4530. 4,161 (4), ainaddetermination of and cation a,A,e l 21) Gera- (2012). al. et A., dan, fi fl inyipoeet on improvements ciency p.125 (pp. . . rls etse applica- et vertus orales: nentoa ora of Journal International nltclChemistry Analytical and h http://dx.doi.org/10. ukodracepacia Burkholderia , , pigmnssensuSphingomonas ttp://dx.doi.org/10. – – 55 78 164. 170. , , otmnto in contamination 14 Sphingopyxis 4,1721 (4), 7,2128 (7), 240 . – . . 3,229 (3), . 7) Oxford: 178). . Pseudomonas http://dx.doi. http://dx.doi. ple and Applied International . . , Burkholderia 1,76 (1), — 55 review. a (23 , , – – 52 46 – – 1724. 2136. – Food – 234. agar 24), (6), ,on 585 (4), 83. , , , , Version postprint Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript Manuscrit d’auteur / Author manuscript adneg,P . rgt .M (1983). M. A. Wright, & A., P. Vandenbergh, (1986). L. R. Cole, & A., P. Vandenbergh, degradation. linalool for compositions and method Bacterial (1989). A. P. Vandenbergh, 586 lsy . ogle-ia,G,&Scli-aby .(09.Tcpeo,crtn,phe- carotene, Tocopherol, (2009). H. Secilmis-Canbay, & G., Bosgelmez-Tinaz, S., Ulusoy, onk . akr,H,Otr,I,Sgi,O,Bya,O,&Ytm .(01.Ef (2011). H. Yetim, & O., Bayram, O., Sagdic, I., Ozturk, H., Cankurt, F., Tornuk, Journal homepage Food Research International Ve eaoimby metabolism 0099-2240/86/100939-02$02.00/0).(doi: Pseudomonas 1989. 24, Jan. issued 4,800,158 No patent U.S. 009-9475-y oi otnsadatbceilpoete frs seta i,hdoo n absolute. and [Article] hydrosol oil, essential rose of properties antibacterial and contents nolic 1953 Microbiology Salmonella reducing in sanitizers food natural as hydrosols plant ious ytmtcadEouinr Microbiology Evolutionary and Systematic 1099/00207713-51-4-1405 damascena andRosa centifolia) support thegrowth of a heterogeneous spoilage microbiota. Food r correspondance) (2015). Hydrosolsof orangeblossom (Citrusaurantium), androse flower(Rosa si on définitive du m définitive on – 95(o:0099-2240/83/061953-03$02.oo/O). (doi: 1955 Labadie, C.,Ginies, Guinebretière, M.H.,Renard, C.,Cerutti, C., Carlin,F.(Auteur de urn Microbiology Current yhmru nfehctcrosadapples. and carrots cut fresh on Typhimurium . , 1,30 148(1), fl uorescens suooa putida Pseudomonas Research International, 76, 576-586. DOI : 10.1016/j.foodres.2015.07.014 – 35. . ple n niomna Microbiology Environmental and Applied

. : http://www. http://dx.doi.org/10.1016/j.ijfoodmicro.2011.04.022 , anu 59

5,554 (5), . scr lsi novmn nlnlo eaoimby metabolism linalool in involvement Plasmid ple n niomna Microbiology Environmental and Applied it lsi novmn naylcisoprenoid acyclic in involvement Plasmid

, p – (201 51 558. ub 4,1405 (4), elsevier.com/locate lié http://dx.doi.org/10.1007/s00284- Comment citer cedocument: 5 ), e

Vol. s / s dan – .Lbdee l odRsac nentoa 6(05 576 (2015) 76 International Research Food / al. et Labadie C. Escherichia coli 1417. nentoa ora fFood of Journal International

76 http://dx.doi.org/10. Fi - 576 , p. , na 52

4,939 (4), 17H and O157:H7 l ﬁ ayo var- of cacy ver , 586, si / 45 foodres – . on of the ma on of 940 (6), DOI at .(05.Hydrosols (2015). M. Watt, okn .A . a e ef .J 20) eailbiotransformation Geraniol (2001). J. M. Werf, der van & M., A. W. Wolken, h,Y J,Zo,H T,H,Y H,Tn,J Y,S,M X,Go .-. ta.(2011). al. et -J., Y. Guo, -X., M. Su, -Y., J. Tang, -H., Y. Hu, -T., H. Zhou, -J., Y. Zhu, on .C,Ba,R,&Rsl .(02.Foe xrcsadteresnilol spotential as oils essential their and extracts Flower (2012). G. Rusul, & R., Bhat, C., H. Voon, alee . adi,A,Gvr,D,Hny . eBad,E,LPm,J . ta.(2009). al. et J., J. LiPuma, E., Brandt, De D., Henry, D., Gevers, A., Baldwin, E., Vanlaere, : : 10.1016/j.foodres.2015.07.014 sp aromamedical.org . j.foodchem.2010.06.036 acid. 2-phenylacetic and 2-phenylethanol, 2-phenylacetaldehyde of activities antimicrobial and Antityrosinase 1541-4337.2011.00169.x eiw nFo cec n odSafety Food and Science Food applications. pharmaceutical in and Reviews uses food for agents antimicrobial http://dx.doi.org/10.1099/ijs.0.001123-0 731 nentoa ora fSseai n vltoayMicrobiology Evolutionary and Systematic of Journal International species, novel the two within complex a K, Taxon ore – nuscri 737. s of http://dx.doi.org/10.1007/s002530100821 eiilu digitatum Penicillium pt publis ls ceso pi 2015) April on access (last – ukodracontaminans Burkholderia 586 odChemistry Food . — hed hed rdcin&SlsHp.Rtivdfrom Retrieved Hype. Sales & Production . in ukodracepacia Burkholderia ple irbooyadBiotechnology and Microbiology Applied :

, . , 124 11 1,34 (1), 1,298 (1),

p o.and nov. sp. – . – 55. 302. ope,cmrssa least at comprises complex, http://dx.doi.org/10.1111/j. http://dx.doi.org/10.1016/ ukodralata Burkholderia , 59 Comprehensive 1,102 (1), — http://www. aha in pathway , 57 p nov. sp. (5 – 111. – 6),