Phospho-RPS6KA1-S363 Antibody Kit

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Leader in Biomolecular Solutions for Life Science Phospho-RPS6KA1-S363 Antibody kit Catalog No.: RK04083 Basic Information Background Catalog No. This gene encodes a member of the RSK (ribosomal S6 kinase) family of RK04083 serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated Observed MW kinase (MAPK) signalling pathway. The activity of this protein has been implicated in 90kDa controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Calculated MW 72kDa/81kDa/82kDa/83kDa Component Category Catalog No. Product Name Applications Cross-Reactivity Primary antibody AP0767 Phospho-P90RSK- WB, IP Human, Rat S363 Rabbit pAb A15718 P90RSK Rabbit pAb WB,IHC,IF Human, Mouse, Rat Recommended Dilutions AP0767 WB 1:500 - 1:2000 A15718 WB 1:500 - 1:2000 For more information please visit Immunogen Information www.abclonal.com Gene ID Swiss Prot 6195 Q15418 Product Information Immunogen Source Isotype A phospho specific peptide corresponding to residues surrounding S363 of human Rabbit IgG P90RSK A synthetic peptide corresponding to a sequence within amino acids 600 to the C- Purification terminus of human P90RSK (NP_002944.2). Affinity purification Synonyms Storage HU-1;MAPKAPK1A;RSK;RSK1;p90Rsk;RPS6KA1 Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Contact www.abclonal.com Validation Data Western blot analysis of extracts of various cell lines, using P90RSK antibody (A15718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. Western blot analysis of extracts of various cell lines, using Phospho-RPS6KA1-S363 antibody (AP0767) at 1:2000 dilution or RPS6KA1 antibody (A15718). Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum- starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunofluorescence analysis of L929 cells Immunofluorescence analysis of U-2 OS embedded mouse spleen using RPS6KA1 using RPS6KA1 antibody (A15718) at cells using RPS6KA1 antibody (A15718) at antibody (A15718) at dilution of 1:100 (40x dilution of 1:100. Blue: DAPI for nuclear dilution of 1:100. Blue: DAPI for nuclear lens). staining. staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Gene Symbol Gene Description ACVR1B Activin a Receptor, Type IB
Table S1. Kinase clones included in human kinase cDNA library for yeast two-hybrid screening Gene Symbol Gene Description ACVR1B activin A receptor, type IB ADCK2 aarF domain containing kinase 2 ADCK4 aarF domain containing kinase 4 AGK multiple substrate lipid kinase;MULK AK1 adenylate kinase 1 AK3 adenylate kinase 3 like 1 AK3L1 adenylate kinase 3 ALDH18A1 aldehyde dehydrogenase 18 family, member A1;ALDH18A1 ALK anaplastic lymphoma kinase (Ki-1) ALPK1 alpha-kinase 1 ALPK2 alpha-kinase 2 AMHR2 anti-Mullerian hormone receptor, type II ARAF v-raf murine sarcoma 3611 viral oncogene homolog 1 ARSG arylsulfatase G;ARSG AURKB aurora kinase B AURKC aurora kinase C BCKDK branched chain alpha-ketoacid dehydrogenase kinase BMPR1A bone morphogenetic protein receptor, type IA BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) BRAF v-raf murine sarcoma viral oncogene homolog B1 BRD3 bromodomain containing 3 BRD4 bromodomain containing 4 BTK Bruton agammaglobulinemia tyrosine kinase BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) BUB1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) C9orf98 chromosome 9 open reading frame 98;C9orf98 CABC1 chaperone, ABC1 activity of bc1 complex like (S. pombe) CALM1 calmodulin 1 (phosphorylase kinase, delta) CALM2 calmodulin 2 (phosphorylase kinase, delta) CALM3 calmodulin 3 (phosphorylase kinase, delta) CAMK1 calcium/calmodulin-dependent protein kinase I CAMK2A calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha CAMK2B calcium/calmodulin-dependent
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9765 Clairemont Mesa Blvd, Suite C San Diego, CA 92124 Tel: 858.875.1900 Fax: 858.622.0609 Phospho-RPS6KA1(T359) Antibody Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP3497a Specification Phospho-RPS6KA1(T359) Antibody - Product Information Application WB, DB,E Primary Accession Q15418 Other Accession P10666, P10665, P18652 Reactivity Human Predicted Chicken, Xenopus Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 82723 Phospho-RPS6KA1(T359) Antibody - Additional Information Gene ID 6195 Other Names Western blot analysis of extracts from Hela Ribosomal protein S6 kinase alpha-1, cells,untreated or treated with S6K-alpha-1, 90 kDa ribosomal protein S6 TPA,200nM，using phospho RPS6KA1-T359 (left) kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP or RPS6KA1 antibody(right) kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK-1, RPS6KA1, MAPKAPK1A, RSK1 Target/Specificity This RPS6KA1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T359 of human RPS6KA1. Dilution WB~~1:2000 DB~~1:500 Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. Dot blot analysis of anti-RPS6KA1-pT359 Pab (RB13385) on nitrocellulose membrane. 50ng Storage of Phospho-peptide or Non Phospho-peptide per Maintain refrigerated at 2-8°C for up to 6 dot were adsorbed. Antibody working months. For long term storage store at -20°C concentrations are 0.5ug per ml. in small aliquots to prevent freeze-thaw cycles.
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