Leader in Biomolecular Solutions for Life Science Phospho-RPS6KA1-S363 Antibody kit Catalog No.: RK04083 Basic Information Background Catalog No. This gene encodes a member of the RSK (ribosomal S6 kinase) family of RK04083 serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated Observed MW kinase (MAPK) signalling pathway. The activity of this protein has been implicated in 90kDa controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Calculated MW 72kDa/81kDa/82kDa/83kDa Component Category Catalog No. Product Name Applications Cross-Reactivity Primary antibody AP0767 Phospho-P90RSK- WB, IP Human, Rat S363 Rabbit pAb A15718 P90RSK Rabbit pAb WB,IHC,IF Human, Mouse, Rat Recommended Dilutions AP0767 WB 1:500 - 1:2000 A15718 WB 1:500 - 1:2000 For more information please visit Immunogen Information www.abclonal.com Gene ID Swiss Prot 6195 Q15418 Product Information Immunogen Source Isotype A phospho specific peptide corresponding to residues surrounding S363 of human Rabbit IgG P90RSK A synthetic peptide corresponding to a sequence within amino acids 600 to the C- Purification terminus of human P90RSK (NP_002944.2). Affinity purification Synonyms Storage HU-1;MAPKAPK1A;RSK;RSK1;p90Rsk;RPS6KA1 Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Contact www.abclonal.com Validation Data Western blot analysis of extracts of various cell lines, using P90RSK antibody (A15718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. Western blot analysis of extracts of various cell lines, using Phospho-RPS6KA1-S363 antibody (AP0767) at 1:2000 dilution or RPS6KA1 antibody (A15718). Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum- starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunofluorescence analysis of L929 cells Immunofluorescence analysis of U-2 OS embedded mouse spleen using RPS6KA1 using RPS6KA1 antibody (A15718) at cells using RPS6KA1 antibody (A15718) at antibody (A15718) at dilution of 1:100 (40x dilution of 1:100. Blue: DAPI for nuclear dilution of 1:100. Blue: DAPI for nuclear lens). staining. staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..