UHPLC High Resolution Orbitrap Metabolomic Fingerprinting of The
G Model BJP-324; No. of Pages 9 ARTICLE IN PRESS Revista Brasileira de Farmacognosia xxx (2016) xxx–xxx ww w.elsevier.com/locate/bjp Original Article UHPLC high resolution orbitrap metabolomic fingerprinting of the unique species Ophryosporus triangularis Meyen from the Atacama Desert, Northern Chile a,∗ b c a d Mario J. Simirgiotis , Cristina Quispe , Andrei Mocan , José Miguel Villatoro , Carlos Areche , e f g,h Jorge Bórquez , Beatriz Sepúlveda , Carlos Echiburu-Chau a Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile b Instituto de Etnofarmacología, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique, Chile c Department of Pharmaceutical Botany, Iuliu Hat¸ieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania d Departamento de Química, Facultad de Ciencias, Universidad de Chile, Santiago, Chile e Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Antofagasta, Chile f Departamento de Ciencias Químicas, Universidad Andres Bello, Campus Vi˜na del Mar, Vi˜na del Mar, Chile g Centro de Investigaciones del Hombre en el Desierto (CIHDE), Arica, Chile h Facultad de Ciencias de la Salud, Universidad de Tarapacá, Arica, Chile a b s t r a c t a r t i c l e i n f o Article history: High-resolution mass spectrometry is currently used to determine the mass of biologically active com- Received 20 May 2016 pounds in plants and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and Accepted 28 October 2016 metabolomics analysis. In this work, several phenolic compounds including eleven phenolic acids, two Available online xxx fatty acids, two chromones and fourteen flavones were rapidly identified in the methanolic extracts of aerial parts and flowers of the unique Chilean species Ophryosporus triangularis Meyen, Asteraceae, Keywords: growing in the Atacama Desert by means of ultrahigh resolution liquid chromatography orbitrap MS UHPLC analysis (UHPLC-PDA-OT-MS) for the first time.
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