Contents • November 2020 • Volume 58, No. 11

CONTENTS • NOVEMBER 2020 • VOLUME 58, NO. 11

PHOTO QUIZ Erik Munson R Photo Quiz: Bilateral Necrotizing Pneumonia in a 30-Year-Old Woman, a Hairy e00451-20 Photo Quiz Situation Abdulaziz M. Almulhim, Cristina Marie Vellozzi-Averhoff, Jessica Howard-Anderson, Ahmed Babiker, Colleen S. Kraft Erik Munson R Answer to Photo Quiz e00454-20 Photo Quiz Abdulaziz M. Almulhim, Cristina Marie Vellozzi-Averhoff, Jessica Howard-Anderson, Ahmed Babiker, Colleen S. Kraft

EDITORIAL Melissa B. Miller R Can We Test Our Way Out of the COVID-19 Pandemic? e02225-20 Editorial Matthew A. Pettengill, Alexander J. McAdam

THE BRIEF CASE Alexander J. R The Brief Case: Cough in an Immunocompromised Patient e00369-20 McAdam BRIEF CASE Anita Shallal, Norman Markowitz, Robert Tibbetts Alexander J. R Closing the Brief Case: Cough in an Immunocompromised Patient e00370-20 McAdam Brief Case Anita Shallal, Norman Markowitz, Robert Tibbetts

BIOGRAPHICAL FEATURE Alexander J. R Biographical Feature: William A. Hinton, M.D. e01933-20 McAdam Bio Feature Erik Munson

COMMENTARIES Colleen Suzanne R Challenges and Controversies to Testing for COVID-19 e01695-20 Kraft Commentary Matthew J. Binnicker John P. Dekker R Targeted Metagenomics Offers Insights into Potential Tick-Borne Pathogens e01893-20 Commentary Matthew Thoendel Yi-Wei Tang R Evaluation of High-Throughput Serological Tests for SARS-CoV-2 e02179-20 Commentary Michael J. Loeffelholz

Instructions to Authors are available on the journal website. CONTENTS • NOVEMBER 2020 • VOLUME 58, NO. 11

BACTERIOLOGY John P. Dekker T Targeted Metagenomics for Clinical Detection and Discovery of Bacterial Tick-Borne e00147-20 Bacteriology Pathogens Luke Kingry, Sarah Sheldon, Stephanie Oatman, Bobbi Pritt, Melissa Anacker, Jenna Bjork, David Neitzel, Anna Strain, Jon Berry, Lynne Sloan, Laurel Respicio-Kingry, Elizabeth Dietrich, Karen Bloch, Abelardo Moncayo, Ganesh Srinivasamoorthy, Bin Hu, Alison Hinckley, Paul Mead, Kiersten Kugeler, Jeannine Petersen Nathan A. T Phenotypic Detection and Differentiation of Carbapenemase Classes Including e00171-20 Ledeboer Bacteriology OXA-48-Like Enzymes in Enterobacterales and Pseudomonas aeruginosa by a Highly Specialized Micronaut-S Microdilution Assay Niels Pfennigwerth, Sören G. Gatermann, Barbara Körber-Irrgang, Robert Hönings John P. Dekker T Plasma Metagenomic Next-Generation Sequencing Assay for Identifying Pathogens: e00794-20 Bacteriology a Retrospective Review of Test Utilization in a Large Children’s Hospital Denver T. Niles, Dona S. S. Wijetunge, Debra L. Palazzi, Ila R. Singh, Paula A. Revell Erik Munson T Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a e01171-20 Bacteriology Comparison of Three Anatomical Sites Brian M. J. W. van der Veer, Christian J. P. A. Hoebe, Nicole H. T. M. Dukers-Muijrers, Lieke B. van Alphen, Petra F. G. Wolffs Geoffrey A. Land T Enhancement of Culture of Legionella longbeachae from Respiratory Samples by e01218-20 Bacteriology Use of Immunomagnetic Separation and Antimicrobial Decontamination Ali Mohammadi, Stephen T. Chambers, Amy Scott-Thomas, John G. Lewis, Trevor Anderson, Ros Podmore, Jonathan Williman, David Murdoch, Sandy Slow Nathan A. T Rapid Detection and Characterization of Carbapenemases in Enterobacterales e01370-20 Ledeboer Bacteriology with a New Modified Carbapenem Inactivation Method, mCIMplus Morgane Petit, François Caméléna, Aurélie Cointe, Thibaut Poncin, Manel Merimèche, Stéphane Bonacorsi, André Birgy, Béatrice Berçot Erik Munson T Testing of In Vitro Susceptibility of Neisseria gonorrhoeae to Azithromycin: e01398-20 Bacteriology Comparison of Disk Diffusion and Reference Agar Dilution Methods Ricardo Gianecini, Lucia Irazu, Marcelo Rodríguez, Paula Cristaldo, Claudia Oviedo, Marisa Turco, Veronica Rodrigo, Liliana Fernández Canigia, Ana Schneider, Liliana Guelfand, Laura Scocozza, GASSP-AR Working Group, Patricia Galarza Carey-Ann D. T Time to Result for Pathogen Identification and Antimicrobial Susceptibility e01468-20 Burnham Bacteriology Testing of Bronchoalveolar Lavage and Endotracheal Aspirate Specimens in U.S. Acute Care Hospitals Shawn H. MacVane, Niels Oppermann, Romney M. Humphries Nathan A. T Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and e01493-20 Ledeboer Bacteriology Shigella-Unique O-Antigen Biosynthesis Gene Clusters Atsushi Iguchi, Hironobu Nishii, Kazuko Seto, Jiro Mitobe, Kenichi Lee, Noriko Konishi, Hiromi Obata, Taisei Kikuchi, Sunao Iyoda Daniel J. Diekema T Laboratory-Based Surveillance of Clostridium difficile Infection in Australian e01552-20 Bacteriology Health Care and Community Settings, 2013 to 2018 Stacey Hong, Papanin Putsathit, Narelle George, Christine Hemphill, Peter G. Huntington, Tony M. Korman, Despina Kotsanas, Monica Lahra, Rodney McDougall, Casey V. Moore, Graeme R. Nimmo, Louise Prendergast, Jennifer Robson, Lynette Waring, Michael C. Wehrhahn, Gerhard F. Weldhagen, Richard M. Wilson, Thomas V. Riley, Daniel R. Knight

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Daniel J. Diekema T A Phylogeny-Informed Proteomics Approach for Species Identiﬁcation within the e01741-20 Bacteriology Burkholderia cepacia Complex Honghui Wang, Ousmane H. Cissé, Thomas Bolig, Steven K. Drake, Yong Chen, Jeffrey R. Strich, Jung-Ho Youn, Uchenna Okoro, Avi Z. Rosenberg, Junfeng Sun, John J. LiPuma, Anthony F. Suffredini, John P. Dekker

MYCOBACTERIOLOGY AND AEROBIC ACTINOMYCETES Geoffrey A. Land T Characterization of Genomic Variants Associated with Resistance to Bedaquiline e01304-20 Mycobacteriology and Delamanid in Naive Mycobacterium tuberculosis Clinical Strains S. Battaglia, A. Spitaleri, A. M. Cabibbe, C. J. Meehan, C. Utpatel, N. Ismail, S. Tahseen, A. Skrahina, N. Alikhanova, S. M. Mostofa Kamal, A. Barbova, S. Niemann, R. Groenheit, A. S. Dean, M. Zignol, L. Rigouts, D. M. Cirillo Melissa B. Miller T Laboratory Evaluation of a Lateral-Flow Cell for Molecular Detection of First-Line e01417-20 Mycobacteriology and Second-Line Antituberculosis Drug Resistance Donald G. Catanzaro, Rebecca E. Colman, Yvonne Linger, Sophia B. Georghiou, Alexander V. Kukhtin, Marva Seifert, Rebecca C. Holmberg, Haifa Mshaiel, Peter Chiles, Naomi Hillery, Christopher G. Cooney, Timothy C. Rodwell Alexander T Population Structure of Mycobacterium bovis in Germany: a Long-Term Study e01573-20 Mellmann Mycobacteriology Using Whole-Genome Sequencing Combined with Conventional Molecular Typing Methods Thomas A. Kohl, Katharina Kranzer, Sönke Andres, Thierry Wirth, Stefan Niemann, Irmgard Moser

MYCOLOGY Kimberly E. HansonT Rapid Detection of Azole-Resistant Aspergillus fumigatus in Clinical and e00843-20 Mycology Environmental Isolates by Use of a Lab-on-a-Chip Diagnostic System Ling-Shan Yu, Jesus Rodriguez-Manzano, Nicolas Moser, Ahmad Moniri, Kenny Malpartida-Cardenas, Nicholas Miscourides, Thomas Sewell, Tatiana Kochina, Amelie Brackin, Johanna Rhodes, Alison H. Holmes, Matthew C. Fisher, Pantelis Georgiou Kimberly E. HansonT Performance of the Lateral Flow Assay and the Latex Agglutination Serum e01563-20 Mycology Cryptococcal Antigen Test in Cryptococcal Disease in Patients with and without HIV Matthew A. Hevey, Ige A. George, Adriana M. Rauseo, Lindsey Larson, William Powderly, Andrej Spec

PARASITOLOGY Bobbi S. Pritt T Evaluation of Cysteine Protease C of Leishmania donovani in Comparison with e00213-20 Parasitology Glycoprotein 63 and Elongation Factor 1␣ for Diagnosis of Human Visceral Leishmaniasis and for Posttreatment Follow-Up Response Nicky Didwania, Sarfaraz Ahmad Ejazi, Rudra Chhajer, Abdus Sabur, Saumyabrata Mazumder, Mohd Kamran, Raunak Kar, Krishna Pandey, Vidya Nand Ravi Das, Pradeep Das, Mehebubar Rahaman, Rama Prosad Goswami, Nahid Ali Bobbi S. Pritt T Utility of the Rapid Antigen Detection Test E. histolytica Quik Chek for the e01991-20 Parasitology Diagnosis of Entamoeba histolytica Infection in Nonendemic Situations Yasuaki Yanagawa, Rieko Shimogawara, Tomoyuki Endo, Rika Fukushima, Hiroyuki Gatanaga, Kasumi Hayasaka, Yoshimi Kikuchi, Taiichiro Kobayashi, Michiko Koga, Tomohiko Koibuchi, Toshikazu Miyagawa, Ayaka Nagata, Hirotomo Nakata, Shinichi Oka, Risa Otsuka, Kazumi Sakai, Mami Shibuya, Hiroyuki Shingyochi, Etsuko Tsuchihashi, Koji Watanabe, Kenji Yagita

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VIROLOGY Yi-Wei Tang T Measles Vaccine-Associated Rash Illness in China: an Emerging Issue in the e01472-20 Virology Process of Measles Elimination Aili Cui, Huiling Wang, Zhen Zhu, Naiying Mao, Jinhua Song, Yan Zhang, Wenbo Xu Michael J. T Evidence for Missing Positive Results for Human Papilloma Virus 45 (HPV-45) and e01626-20 Loeffelholz Virology HPV-59 with the SPF10-DEIA-LiPA25 (Version 1) Platform Compared to Type-Speciﬁc Real-Time Quantitative PCR Assays and Impact on Vaccine Effectiveness Estimates Kahren van Eer, Suzan Leussink, Tim T. Severs, Naomi van Marm-Wattimena, Petra J. Woestenberg, Johannes A. Bogaards, Audrey J. King Alexander J. T Low-Cost Polyester-Tipped Three-Dimensionally Printed Nasopharyngeal Swab e01668-20 McAdam Virology for the Detection of Severe Acute Respiratory Syndrome-Related Coronavirus 2 (SARS-CoV-2) Mohammad Alghounaim, Sulaiman Almazeedi, Sarah Al Youha, Jesse Papenburg, Osama Alowaish, Ghadeer AbdulHussain, Rawan Al-Shemali, Ahmad Albuloushi, Saud Alzabin, Khaled Al-Wogayan, Yousef Al-Mutawa, Salman Al-Sabah Brad Fenwick T Serological Screening of Inﬂuenza A Virus Antibodies in Cats and Dogs Indicates e01689-20 Virology Frequent Infection with Different Subtypes Shan Zhao, Nancy Schuurman, Malte Tieke, Berit Quist, Steven Zwinkels, Frank J. M. van Kuppeveld, Cornelis A. M. de Haan, Herman Egberink Michael J. T Analysis of Inactivation of SARS-CoV-2 by Specimen Transport Media, Nucleic e01713-20 Loeffelholz Virology Acid Extraction Reagents, Detergents, and Fixatives Stephen R. Welch, Katherine A. Davies, Hubert Buczkowski, Nipunadi Hettiarachchi, Nicole Green, Ulrike Arnold, Matthew Jones, Matthew J. Hannah, Reah Evans, Christopher Burton, Jane E. Burton, Malcolm Guiver, Patricia A. Cane, Neil Woodford, Christine B. Bruce, Allen D. G. Roberts, Marian J. Killip Alexander J. T Self-Collected Anterior Nasal and Saliva Specimens versus Health Care e01824-20 McAdam Virology Worker-Collected Nasopharyngeal Swabs for the Molecular Detection of SARS-CoV-2 K. E. Hanson, A. P. Barker, D. R. Hillyard, N. Gilmore, J. W. Barrett, R. R. Orlandi, S. M. Shakir Randall Hayden T A Direct Comparison of Enhanced Saliva to Nasopharyngeal Swab for the e01946-20 Virology Detection of SARS-CoV-2 in Symptomatic Patients Gary W. Procop, Nabin K. Shrestha, Sherilynn Vogel, Kelly Van Sickle, Susan Harrington, Daniel D. Rhoads, Brian P. Rubin, Paul Terpeluk Alexander J. T Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans e02107-20 McAdam Virology during a Fishery Vessel Outbreak with a High Attack Rate Amin Addetia, Katharine H. D. Crawford, Adam Dingens, Haiying Zhu, Pavitra Roychoudhury, Meei-Li Huang, Keith R. Jerome, Jesse D. Bloom, Alexander L. Greninger

IMMUNOASSAYS Alexander J. T SARS-CoV-2 Assays To Detect Functional Antibody Responses That Block ACE2 e01533-20 McAdam Immunoassays Recognition in Vaccinated Animals and Infected Patients Susanne N. Walker, Neethu Chokkalingam, Emma L. Reuschel, Mansi Purwar, Ziyang Xu, Ebony N. Gary, Kevin Y. Kim, Michaela Helble, Katherine Schultheis, Jewell Walters, Stephanie Ramos, Kar Muthumani, Trevor R. F. Smith, Kate E. Broderick, Pablo Tebas, Ami Patel, David B. Weiner, Daniel W. Kulp

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Yi-Wei Tang T Detection of SARS-CoV-2 IgG Targeting Nucleocapsid or Spike Protein by Four e01742-20 Immunoassays High-Throughput Immunoassays Authorized for Emergency Use Harry E. Prince, Tara S. Givens, Mary Lapé-Nixon, Nigel J. Clarke, Dale A. Schwab, Hollis J. Batterman, Robert S. Jones, William A. Meyer III, Hema Kapoor, Charles M. Rowland, Farnoosh Haji-Sheikhi, Elizabeth M. Marlowe

CLINICAL VETERINARY MICROBIOLOGY Brad Fenwick T Whole-Genome Sequencing of Porcine Reproductive and Respiratory Syndrome e00097-20 Clin Vet Micro Virus from Field Clinical Samples Improves the Genomic Surveillance of the Virus Christian Lalonde, Chantale Provost, Carl A. Gagnon

LETTERS TO THE EDITOR Yi-Wei Tang D High Incidence of False-Positive Results in Patients with Acute Infections Other e01352-20 Letter than COVID-19 by the Liaison SARS-CoV-2 Commercial Chemiluminescent Microparticle Immunoassay for Detection of IgG Anti-SARS-CoV-2 Antibodies Narjis Boukli, Melchior Le Mene, Aurélie Schnuriger, Nancy Stella Cuervo, Cécilia Laroche, Laurence Morand-Joubert, Joël Gozlan Yi-Wei Tang D Evaluation of IgM Lateral Flow Assay as a Screening Tool for Mycoplasma e01498-20 Letter pneumoniae Infection in Childhood Pneumonia Patrick M. Meyer Sauteur, Elena Pánisová, Lucas M. Bachmann, Lilliam Ambroggio, Christoph Berger Alexander J. L Expert Opinion on Veriﬁcation of Antimicrobial Susceptibility Tests e00945-20 McAdam Letter Jean B. Patel, Richard B. Thomson, Kevin Alby, Esther Babady, Karissa Culbreath, Marcelo F. Galas, Shawn R. Lockhart, Brian V. Lubbers, Margie Morgan, Sandra S. Richter, Susan Sharp, Ribhi M. Shawar, Ana Maria Cardenas, German Esparza, Nicole Hubbard, Mark G. Papich, Audrey N. Schuetz

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