Junjie U. Guo, Ph.D

Home , Gao (surname), Sun (surname), Su (surname), Zhou (surname)

Curriculum Vitae (updated 8/23/2020) Junjie U. Guo, Ph.D.

295 Congress Ave. Email: [email protected] Birth: 10/1983, Guangzhou, China BCMM 154B (office) 141 (lab) Office: 203-737-4439 Citizenship: China New Haven, CT 06519 Lab: 203-785-7387 U.S. permanent resident

Employment

09/2017–present Assistant Professor (tenure-track) Department of Neuroscience Yale University School of Medicine

09/2012–08/2017 Damon Runyon Postdoctoral Fellow Whitehead Institute for Biomedical Research / MIT / HHMI Advisor: David P. Bartel, Ph.D.

Education

08/2006–08/2012 Ph.D. in Neuroscience (12/30/2011) The Solomon H. Snyder Department of Neuroscience Johns Hopkins University School of Medicine Thesis: Activity-dependent regulation of DNA methylation in the adult brain Advisor: Hongjun Song, Ph.D.

09/2002–07/2006 B.S. in Biological Sciences (7/1/2006) College of Life Sciences, Peking University, China Thesis: The unusual stability of bacterial periplasmic proteins: a comparison between two differentially localized trehalases Advisor: Zengyi Chang, Ph.D.

Honors

2019 NARSAD Young Investigator Award, Brain & Behavior Research Foundation 2018 NIH Director’s New Innovator (DP2) Award, NIH Common Fund, NIGMS 2018 Klingenstein-Simons Fellowship in Neuroscience, the Esther A. & Joseph Klingenstein Fund and the Simons Foundation 2017 Yale Scholar in Neurodegenerative Research, Yale School of Medicine 2016 Margaret and Herman Sokol Postdoctoral Award, Whitehead Institute 2016 Finalist, Damon Runyon-Dale F. Frey Award for Breakthrough Scientists, Damon Runyon Cancer Research Foundation 2016 Poster Award, Gordon Research Conference on Post-Transcriptional Gene Regulation 2013 Damon Runyon Postdoctoral Fellow, Damon Runyon Cancer Research Foundation 2012 The Peter and Patricia Gruber International Research Award in Neuroscience, Society for Neuroscience, the Gruber Foundation 2012 Harold M. Weintraub Graduate Student Award, Fred Hutchinson Cancer Research Center 2012 The Mette Strand Research Award, Johns Hopkins University 2012 Future of Science Scholarship, Keystone Symposium on Epigenomics 2010–2011 Foundation for Advanced Research in Medical Sciences Fellowship, Johns Hopkins University 2009 NIMH Scholarship, Keystone Symposium on Epigenetics of Neurodevelopmental Disorders 2006 Graduate with High Honor, Peking University 2004–2006 Chun-Tsung Undergraduate Research Fellow, Peking University 2002–2005 Cyrus Tang Fellowship, Peking University

Publications (Primary Research) *, equal contribution; †, corresponding author

1. Sun Y, Eshov A, Zhou J, Isiktas AU, Guo JU†, C9orf72 arginine-rich dipeptide repeats inhibit UPF1-mediated RNA decay via translational repression. Nature Communications, 2020 Jul 3;11(1):3354. PMID: 32620797

2. Guo JU, Bartel DP†, RNA G-quadruplexes are globally unfolded in eukaryotic cells and depleted in bacteria. Science (Research Article), 2016 Sep 23;353(6306):aaf5371. PMID:27708011 Highlighted in Chemical & Engineering News, Nature Chemical Biology, Scivit News, The Scientist

3. Zeng Y, Yao B, Shin J, Lin L, Kim N, Song Q, Liu S, Su Y, Guo JU, Huang L, Wan J, Wu H, Qian J, Cheng X, Zhu H, Ming GL, Jin P, Song H†, Lin28A Binds Active Promoters and Recruits Tet1 to Regulate Gene Expression. Molecular Cell, 2016 Jan 7;61(1):153-60. PMID:267110097

4. Yu H, Su Y, Shin J, Zhong C, Guo JU, Weng YL, Gao F, Geschwind DH, Ming GL, Song H†, Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair. Nature Neuroscience, 2015 Jun;18(6):836-43. PMID:25915473

5. Guo JU, Agarwal V, Guo H, Bartel DP†, Expanded identification and characterization of mammalian circular RNAs. Genome Biology, 2014 Jul 29;15(7):409. PMID:25070500

6. Chang YI, Damnernsawad A, Allen LK, Yang D, Ranheim EA, Young KH, Zhang J, Kong G, Wang J, Liu Y, Fu HY, Yang CS, Guo J, Song H, Zhang J†, Evaluation of allelic strength of human TET2 mutations and cooperation between Tet2 knockdown and oncogenic NRAS mutation. British Journal of Haematology, 2014 Apr 3. doi: 10.1111/bjh.12871. PMID:24697267

7. Guo JU*, Su Y*, Shin J, Xie B, Zhong C, Le T, Fan G, Zhu H, Chang Q, Ming GL, Gao Y, Song H†, Distribution, recognition and regulation of non-CpG methylation in the adult mammalian brain. Nature Neuroscience, 2014 Feb;17(2):215-22. PMID: 24362762

8. Yeo M, Berglund K, Hanna M, Guo JU, Abramowitz J, Busciglio J, Gao Y, Birnbaumer L, Liedtke WB†, Bisphenol A delays the perinatal chloride shift in cortical neurons by epigenetic effects on the Kcc2/Slc12a5 promoter. Proc Natl Acad Sci U S A., 2013 Mar 12;110(11):4315-4320. PMID:23440186

9. Jang MH, Bonaguidi MA, Kitabatake Y, Sun J, Song J, Kang E, Jun H, Zhong C, Su Y, Guo JU, Wang MX, Sailor KA, Kim JY, Gao Y, Christian KM, Ming GL, Song H†, Secreted frizzled-related protein 3 regulates activity-dependent adult hippocampal neurogenesis. Cell Stem Cell, 2013 Feb 7;12(2):215-23. PMID:23395446

10. Kang E, Burdick KE, Kim JY, Duan X, Guo JU, Sailor KA, Jung DE, Ganesan S, Choi S, Pradhan D, Lu B, Dimitrios A, Christian K, Malhotra AK, Song H, Ming GL†, Interaction between FEZ1 and DISC1 in regulation of neuronal development and risk for schizophrenia. Neuron, 2011 Nov 17; 72(4):559-71. PMID:22099459

11. Guo JU, Ma DK, Mo H, Ball MP, Jang MH, Bonaguidi MA, Balazer JA, Eaves HL, Xie B, Ford E, Zhang K, Ming GL, Gao Y, Song H†, Neuronal activity modifies the DNA methylation landscape in the adult brain. Nature Neuroscience, 2011 Aug 28;14(10):1345-51. PMID:21874013

12. Guo JU, Su Y, Zhong C, Ming GL, Song H†, 2

Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Cell, 2011 Apr 29; 145(3):423-34. PMID:21496894

13. Kim JY, Duan X, Liu CY, Jang MH, Guo JU, Pow-anpongkul N, Kang E, Song H, Ming GL†, DISC1 regulates new neuron development in the adult brain via modulation of AKT-mTOR signaling through KIAA1212. Neuron, 2009 Sep 24; 63(6): 761-773 PMID:19778506

14. Ma DK, Jang MH, Guo JU, Kitabatake Y, Pow-anpongkul N, Flavell RA, Lu B, Ming GL, Song H†, Neuronal activity-induced Gadd45b promotes epigenetic DNA demethylation and adult neurogenesis. Science, 2009 Feb 20;323(5917):1074-7. PMID:19119186

Publications (Invited Reviews and Commentaries) †, corresponding author

1. Wei LH, Guo JU†, Coding functions of “noncoding” RNAs. Science, 2020 Mar 6;367(6482):1074-1075. PMID: 32139529

2. Felling RJ, Guo JU, Song H†, Neuronal activation and insight into the plasticity of DNA methylation. Epigenomics, 2012 Apr;4(2):125-7. PMID:22449183

3. Guo JU, Su Y, Zhong C, Ming GL, Song H†, Emerging roles of TET proteins and 5-hydroxymethylcytosines in active DNA demethylation and beyond. Cell Cycle, 2011 Aug 15; 10(16):2662-8. PMID:21811096

4. Ma DK, Marchetto MC, Guo JU, Ming GL, Gage FH, Song H†, Epigenetic choreographers of neurogenesis in the adult mammalian brain. Nature Neuroscience, 2010 Nov;13(11):1338-44. PMID:20975758

5. Ma DK, Guo JU, Ming GL, Song H†, DNA excision repair proteins and Gadd45 as molecular players for active DNA demethylation. Cell Cycle, 2009 May 20;8(10):1526-31. PMID:19377292

Book Chapters

1. Guo JU, Ming GL, Song H, Chapter “Active DNA Demethylation and 5-Hydroxymethylcytosine”, Epigenetic Regulation in the Nervous System: Basic Mechanisms and Clinical Impact, Elsevier Science, 2013

2. Ma DK, Guo JU, Ming GL, Song H, Chapter “Bridging Environment and DNA: Activity-Induced Epigenetic Modification in the Adult Brain”, Two Faces of Evil: Cancer and Neurodegeneration, Springer-Verlag Berlin Heidelberg, 2011

Presentations

2020 Online seminar, RNA Collaborative Seminar Series, Yale Center for RNA Sciences and Medicine 2020 Online seminar, “C9ALS/FTD-associated dipeptide repeats inhibit nonsense-mediated decay and cause neurodegeneration”, 25th Annual Meeting of RNA Society 2019 Seminar, “Molecular determinants of ALS/FTD-associated non-AUG translation”, Kavli Symposium on Neurodegeneration, Yale School of Medicine 2018 Short talk, “Molecular determinants of ALS/FTD-associated non-AUG translation”, Gordon Research Conference on Post-Transcriptional Gene Regulation 2018 Invited seminar, “Intracellular folding of G-quadruplex regions in cellular and ALS-associated repeat 3

RNAs”, the Robert Packard Center for ALS Research, Johns Hopkins University 2016 Invited seminar, “The folding landscape of RNA G-quadruplexes in cells”, RNA & Disease Symposium, ETH Zurich 2016 Seminar, “Cellular remodeling of a noncanonical RNA structure”, Whitehead Forum 2015 Short talk, “The folding landscape of RNA G-quadruplexes in cells”, Damon Runyon Symposium 2012 Seminar, “A non-canonical form of DNA methylation in the mammalian brain”, Whitehead Forum 2010 Short talk, "Regulation of DNA methylation in the adult nervous system", Johns Hopkins University Department of Neuroscience Retreat 2010 Short talk, "Regulation of DNA methylation in the adult nervous system", Johns Hopkins Brain Science Institute Mini-symposium on Neural Regeneration

Mentoring

Postdoctoral associates Lian-Huan Wei (2019−), Chethan Reddy (2018−), Yu Sun (2017−)

Postgraduate associate Atagun Isiktas, (2019−), Aziz Eshov (2017−2019)

PhD students Suzhou Yang (INP, 2020−), Paul Jongseo Lee (INP, 2020−)

Undergraduates Nicholas DeRuiter (MCDB, 2019−2020), Jeffrey Zhou (MB&B, 2017−2020, ThermoFisher Scientific Scholarship, Yale Summer Research Fellowship), Nicholas DeWalt (MB&B, 2019−2020), Tuzo Mwarumba (2019, Yale BioMed Amgen Scholar)

Thesis committee Leon Tejwani (INP, 2019−), Tianshuo Liu (MCDB, 2019−), Han Wan (MCDB, 2019−), Carrie Ann Davison (Genetics, 2020−), Wenqing Cao (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 2020), Ethan Stray (Genetics, 2020−)

Qualify exam committee Mick Park (INP, 2018), Sachira Denagamage (INP, 2019), Kathy Zhang (INP, 2019), Corbin Renken (Cell Bio, 2019), Carrie Ann Davison (Genetics, 2019), Kim Luttik (INP, 2019), Tianshuo Liu (MCDB, 2019) , Han Wan (MCDB, 2019), Shengyan (Tiffani) Jin (Genetics, 2020)

Teaching

2020 Principles of Neuroscience, Yale School of Medicine 2020 Cellular and Molecular Mechanisms of Neurological Diseases, YSM 2018– Foundation in Cellular and Molecular Neurobiology, Yale School of Medicine 2011–2012 Guest lecture, Biotechnology Training Course 39: Epigenetics, NIH 2011 Teaching Assistant, Stem Cells: Unit of Development and Regeneration, JHU 2008–2009 Teaching Assistant, Neuroscience and Neuroanatomy, Johns Hopkins University

Other Professional Affiliations and Services

Organizer Yale Neuroscience Research in Progress seminar series (2019–) Organizer The 8th Annual Retreat of Yale Center for RNA Sciences and Medicine (2019) Grant reviewer ETH Zurich (2014), Swiss National Science Foundation (2018, 2019), Motor Neuron Disease Association (2019) Ad hoc reviewer Science, Neuron, Nature Chemical Biology, Nature Communications, Cell Reports, Nucleic Acids Research, Annals of Neurology, Journal of Biological Chemistry, Epigenetics & Chromatin, Human Molecular Genetics, RNA, Stem Cells, Bioinformatics, Carcinogenesis, Apoptosis, Neuropharmacology, Journal of Cerebral Blood Flow & Metabolism, Lab on a Chip External reviewer Five-year research evaluation, Czech Academy of Sciences (2020) 4

Admission committee Interdepartmental Neuroscience Program (2019–) Associate member Dean’s Advisory Council on LGBTQI+ Affairs, Yale School of Medicine (2017–) Affiliated faculty Program in Cellular Neuroscience, Neurodegeneration and Repair, YSM (2018–) Member Society for Neuroscience (2006–), RNA Society (2017–), Yale Center for RNA Science and Medicine (2017–)