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A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia
Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2019 A phylogenomic analysis of the floral transcriptomes of sexually deceptive and rewarding European orchids, Ophrys and Gymnadenia Pineiro Fernandez, Laura ; Byers, Kelsey J R P ; Cai, Jing ; Sedeek, Khalid E M ; Kellenberger, Roman T ; Russo, Alessia ; Qi, Weihong ; Aquino Fournier, Catharine ; Schlüter, Philipp M Abstract: The orchids (Orchidaceae) constitute one of the largest and most diverse families of flowering plants. They have evolved a great variety of adaptations to achieve pollination by a diverse group of pollinators. Many orchids reward their pollinators, typically with nectar, but the family is also well- known for employing deceptive pollination strategies in which there is no reward for the pollinator, in the most extreme case by mimicking sexual signals of pollinators. In the European flora, two examples of these different pollination strategies are the sexually deceptive genus Ophrys and the rewarding genus Gymnadenia, which differ in their level of pollinator specialization; Ophrys is typically pollinated by pseudo-copulation of males of a single insect species, whilst Gymnadenia attracts a broad range of floral visitors. Here, we present and describe the annotated floral transcriptome of Ophrys iricolor, an Andrena- pollinated representative of the genus Ophrys that is widespread throughout the Aegean. Furthermore, we present additional floral transcriptomes of both sexually deceptive and rewarding orchids, specifi- cally the deceptive Ophrys insectifera, Ophrys aymoninii, and an updated floral transcriptome of Ophrys sphegodes, as well as the floral transcriptomes of the rewarding orchids Gymnadenia conopsea, Gym- nadenia densiflora, Gymnadenia odoratissima, and Gymnadenia rhellicani (syn. -
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The Hardy Orchid Society Newsletter No. 21 July 2001 The Hardy Orchid Society Committee is… President: Richard M Bateman Vice-Presidents: Paul Harcourt Davies and Norman Heywood Chairman: Richard Manuel, Wye View Cottage, Leys Hill, Ross-on-Wye, Herefordshire, HR9 5QU Secretary: Sarah Marks, 83 Ladysmith, East Gomeldon, Salisbury, Wilts, SP4 6LE Treasurer: Tony Beresford, Pound Farm, Wearne, Langport, Somerset, TA10 0QJ Membership Secretary: Nick Storer, 17 Orchard Close, Lymm, Cheshire, WA13 9HH Show Secretary: Doreen Webster, 25 Highfields Drive, Loughborough, Leics, LE11 3JS Newsletter Editor: Moira Tarrant, Bumby’s, Fox Rd., Mashbury, Chelmsford, CM1 4TJ Meetings Secretary: Colin Clay, 14 Cromwell Place, Lighthorne Heath, Leamington Spa, CV33 9TG Ordinary Member (publicity): Simon Tarrant, Bumby’s, Fox Rd., Mashbury, Chelmsford, CM1 4TJ Ordinary Member (Newsletter Dist.): Bill Temple, Primrose Cottage, Hanney Rd., Ste- venton, Oxon, OX13 6AP Ordinary Member (Seed & Fungus Bank): Ted Weeks, 74 Over Lane, Almondsbury, Bristol, BS32 4BT Co-opted Member (BOC Rep.): Richard Nicol, 1364 Evesham Rd., Astwood Bank, Red- ditch, Worcs, B96 6BD Contents P.3 From the New President, Richard Bateman P.5 Report of the 9th AGM of the Hardy Orchid Society P.7 Publicity Posters, Simon Tarrant P.8 HELP!, Richard Manuel P.8 HOS Plant Show 2001, Tony Hughes P.10 Does DNA reveal All about the Evolution of Terrestrial Orchids? Part 3, Richard Bateman P.14 Getting Started - the Basics of Hardy Orchid Cultivation, Alan Dash P.20 Chemical Warfare, Richard Manuel P.21 AGS Summer South Show - Silver Award, Carol Dash P.22 Seed and Fungus Bank, Ted Weeks Colour Insert between Pages 12 and 13 Cover illustration: Serapias lingua by Carol Dash HOS Newsletter 21, July 2001 From the New President of the HOS Richard Bateman I am of course delighted to accept the Presidency of the Hardy Orchid Society. -
Taxonomic Notes on Anacamptis Pyramidalis Var. Urvilleana (Orchidaceae), a Good Endemic Orchid from Malta
J. Eur. Orch. 48 (1): 19 – 28. 2016. Stephen Mifsud Taxonomic notes on Anacamptis pyramidalis var. urvilleana (Orchidaceae), a good endemic orchid from Malta Keywords Orchidaceae; Anacamptis urvilleana; Anacamptis pyramidalis; Anacamptis pyramidalis var. urvilleana; Maltese endemics; Flora of Malta; Central Mediterranean region. Summary Mifsud S. (2016): Taxonomic notes on Anacamptis pyramidalis var. urvilleana (Orchidaceae), a good endemic orchid from Malta.- J. Eur. Orch. 48 (1): 19-28. In several global plant species databases the Maltese-endemic Anacamptis urvilleana is considered as a synonym of A. pyramidalis, hence reflecting the belief of some European authors. A number of morphological differences and phenology differentiate the Maltese pyramidical orchid from A. pyramidalis. As a result, it is suggested to maintain the identity of this orchid as A. pyramidalis var. urvilleana which merits conservation treatments different from the widely distributed A. pyramidalis s. str. Zusammenfassung Mifsud S. (2016): Taxonomische Anmerkungen zu Anacamptis pyramidalis var. urvilleana (Orchidaceae), eine gute endemische Orchidee von Malta.- J. Eur. Orch. 48 (1): 19-28. In verschiedenen weltweiten Datenbanken botanischer Namen, die auch die Meinung einiger europäischer Autoren wiedergeben, wird der maltesische Endemit Anacamptis urvilleana als Synonym von A. pyramidalis geführt. Die maltesische Pyramiden-Hundswurz unterscheidet sich jedoch sowohl in einer Reihe von morphologischen Merkmalen als auch phenologisch von A. pyramidalis. Auf dieser Grundlage wird vorgeschlagen, diese Orchidee als A. pyramidalis var. urvilleana zu führen. Zu ihrem Schutz sind andere Erhaltungsmaßnahmen erforderlich als für die weitverbreitete A. pyramidalis s. str. Journal Europäischer Orchideen 48 (1): 2016. 19 1. Introduction Anacamptis urvilleana Sommier & Caruana Gatto was described in 1915 (refer Fig.1) as an endemic orchid from the Maltese islands. -
Actes Du 15E Colloque Sur Les Orchidées De La Société Française D’Orchidophilie
Cah. Soc. Fr. Orch., n° 7 (2010) – Actes 15e colloque de la Société Française d’Orchidophilie, Montpellier Actes du 15e colloque sur les Orchidées de la Société Française d’Orchidophilie du 30 mai au 1er juin 2009 Montpellier, Le Corum Comité d’organisation : Daniel Prat, Francis Dabonneville, Philippe Feldmann, Michel Nicole, Aline Raynal-Roques, Marc-Andre Selosse, Bertrand Schatz Coordinateurs des Actes Daniel Prat & Bertrand Schatz Affiche du Colloque : Conception : Francis Dabonneville Photographies de Francis Dabonneville & Bertrand Schatz Cahiers de la Société Française d’Orchidophilie, N° 7, Actes du 15e Colloque sur les orchidées de la Société Française d’Orchidophilie. ISSN 0750-0386 © SFO, Paris, 2010 Certificat d’inscription à la commission paritaire N° 55828 ISBN 978-2-905734-17-4 Actes du 15e colloque sur les Orchidées de la Société Française d’Orchidophilie, D. Prat et B. Schatz, Coordinateurs, SFO, Paris, 2010, 236 p. Société Française d’Orchidophilie 17 Quai de la Seine, 75019 Paris Cah. Soc. Fr. Orch., n° 7 (2010) – Actes 15e colloque de la Société Française d’Orchidophilie, Montpellier Préface Ce 15e colloque marque le 40e anniversaire de notre société, celle-ci ayant vu le jour en 1969. Notre dernier colloque se tenait il y a 10 ans à Paris en 1999, 10 ans c’est long, 10 ans c’est très loin. Il fallait que la SFO renoue avec cette traditionnelle organisation de colloques, manifestation qui a contribué à lui accorder la place prépondérante qu’elle occupe au sein des orchidophiles français et de la communauté scientifique. C’est chose faite aujourd’hui. Nombreux sont les thèmes qui font l’objet de communications par des intervenants dont les compétences dans le domaine de l’orchidologie ne sont plus à prouver. -
Eugenol Synthase Genes in Floral Scent Variation in Gymnadenia Species
CORE Metadata, citation and similar papers at core.ac.uk Provided by RERO DOC Digital Library Funct Integr Genomics (2014) 14:779–788 DOI 10.1007/s10142-014-0397-9 ORIGINAL PAPER Eugenol synthase genes in floral scent variation in Gymnadenia species Alok K. Gupta & Ines Schauvinhold & Eran Pichersky & Florian P. Schiestl Received: 9 July 2013 /Revised: 7 August 2014 /Accepted: 31 August 2014 /Published online: 20 September 2014 # Springer-Verlag Berlin Heidelberg 2014 Abstract Floral signaling, especially through floral scent, is activity in Escherichia coli. While G. odoratissima and often highly complex, and little is known about the molecular Gymnadenia conopsea enzymes were found to catalyze the mechanisms and evolutionary causes of this complexity. In formation of eugenol only, the Gymnadenia densiflora pro- this study, we focused on the evolution of “floral scent genes” teins synthesize eugenol, as well as a smaller amount of and the associated changes in their functions in three closely isoeugenol. Finally, we showed that the eugenol and related orchid species of the genus Gymnadenia.Wedevel- isoeugenol producing gene copies of G. densiflora are evolu- oped a benchmark repertoire of 2,571 expressed sequence tags tionarily derived from the ancestral genes of the other species (ESTs) in Gymnadenia odoratissima. For the functional char- producing only eugenol. The evolutionary switch from pro- acterization and evolutionary analysis, we focused on eugenol duction of one to two compounds evolved under relaxed synthase, as eugenol is a widespread and important scent purifying selection. In conclusion, our study shows the mo- compound. We obtained complete coding complementary lecular bases of eugenol and isoeugenol production and sug- DNAs (cDNAs) of two copies of putative eugenol synthase gests that an evolutionary transition in a single gene can lead genes in each of the three species. -
Phylogenetics of Tribe Orchideae (Orchidaceae: Orchidoideae)
Annals of Botany 110: 71–90, 2012 doi:10.1093/aob/mcs083, available online at www.aob.oxfordjournals.org Phylogenetics of tribe Orchideae (Orchidaceae: Orchidoideae) based on combined DNA matrices: inferences regarding timing of diversification and evolution of pollination syndromes Luis A. Inda1,*, Manuel Pimentel2 and Mark W. Chase3 1Escuela Polite´cnica Superior de Huesca, Universidad de Zaragoza, carretera de Cuarte sn. 22071 Huesca, Spain, 2Facultade de Ciencias, Universidade da Corun˜a, Campus da Zapateira sn. 15071 A Corun˜a, Spain and 3Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK * For correspondence. E-mail [email protected] Received: 3 November 2011 Returned for revision: 9 December 2011 Accepted: 1 March 2012 Published electronically: 25 April 2012 † Background and aims Tribe Orchideae (Orchidaceae: Orchidoideae) comprises around 62 mostly terrestrial genera, which are well represented in the Northern Temperate Zone and less frequently in tropical areas of both the Old and New Worlds. Phylogenetic relationships within this tribe have been studied previously using only nuclear ribosomal DNA (nuclear ribosomal internal transcribed spacer, nrITS). However, different parts of the phylogenetic tree in these analyses were weakly supported, and integrating information from different plant genomes is clearly necessary in orchids, where reticulate evolution events are putatively common. The aims of this study were to: (1) obtain a well-supported and dated phylogenetic hypothesis for tribe Orchideae, (ii) assess appropriateness of recent nomenclatural changes in this tribe in the last decade, (3) detect possible examples of reticulate evolution and (4) analyse in a temporal context evolutionary trends for subtribe Orchidinae with special emphasis on pollination systems. -
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JJoouurrnnaall of the HHAARRDDYY OORRCCHHIIDD SSOOCCIIEETTYY Vol. 6 No. 2 (52) April 2009 JOURNAL of the HARDY ORCHID SOCIETY Vol. 6 No. 2 (52) April 2009 The Hardy Orchid Society Our aim is to promote interest in the study of Native European Orchids and those from similar temperate climates throughout the world. We cover such varied aspects as field study, cultivation and propagation, photography, taxonomy and systematics, and practical conservation. We welcome articles relating to any of these subjects, which will be considered for publication by the editorial committee. Please send your submissions to the Editor, and please structure your text according to the “Advice to Authors” (see website, January 2004 Journal, Members’ Handbook or contact the Editor). Views expressed in journal articles are those of their author(s) and may not reflect those of HOS. The Hardy Orchid Society Committee President: Prof. Richard Bateman, Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, Surrey, TW9 3DS Chairman & Field Meeting Co-ordinator: David Hughes, Linmoor Cottage, Highwood, Ringwood, Hants., BH24 3LE [email protected] Vice-Chairman: Celia Wright, The Windmill, Vennington, Westbury, Shrewsbury, Shropshire, SY5 9RG [email protected] Secretary (Acting): Alan Leck, 61 Fraser Close, Deeping St. James, Peterborough, PE6 8QL [email protected] Treasurer: Iain Wright, The Windmill, Vennington, Westbury, Shrewsbury, Shropshire, SY5 9RG [email protected] Membership Secretary: Celia Wright, The Windmill, Vennington, Westbury, -
Rare Plant Monitoring 2017
RARE PLANT MONITORING 2017 Ajuga pyramidalis Ophrys insectifera © Zoe Devlin What is it? In 2017, we decided to carry out a small pilot scheme on rare plant monitoring. Where experienced plant recorders had submitted recent casual records of rare plants to the Centre, they were asked if they would be willing to visit their rare plant population once a year during its flowering period and to count the total number of individuals present. The response to the scheme from the small number of recorders contacted has been overwhelming positive and it has resulted in very valuable data being collected in 2017. Data on the rare plant location, the count and additional information about the site is submitted online through a dedicated web portal set up by the Data Centre. The project was discussed and agreed with the NPWS. It is framed around the 2016 Vascular Plant Red List and is mainly focused on monitoring vulnerable, near threatened and rare least concern species. Why is it important? When assessing the national FAST FACTS 2017 conservation status of very rare species according to IUCN Red List methodology, it is recommended that 37 you use annual population count data. That’s the total number of rare plant Given the numbers of rare plant populations that were monitored in the species a country might have, this 2017 pilot information can be difficult to collect in any volume. This citizen science project relies on the generosity of 22 expert volunteers to ‘keep an eye’ on That’s the number of rare plant species rare populations near them and to that were monitored in 2017 submit standardised count data once a year. -
“Dynamic Speciation Processes in the Mediterranean Orchid Genus Ophrys L
“DYNAMIC SPECIATION PROCESSES IN THE MEDITERRANEAN ORCHID GENUS OPHRYS L. (ORCHIDACEAE)” Tesi di Dottorato in Biologia Avanzata, XXIV ciclo (Indirizzo Sistematica Molecolare) Universitá degli Studi di Napoli Federico II Facoltá di Scienze Matematiche, Fisiche e Naturali Dipartimento di Biologia Strutturale e Funzionale 1st supervisor: Prof. Salvatore Cozzolino 2nd supervisor: Prof. Serena Aceto PhD student: Dott. Hendrik Breitkopf 1 Cover picture: Pseudo-copulation of a Colletes cunicularius male on a flower of Ophrys exaltata ssp. archipelagi (Marina di Lesina, Italy. H. Breitkopf, 2011). 2 TABLE OF CONTENTS GENERAL INTRODUCTION CHAPTER 1: MULTI-LOCUS NUCLEAR GENE PHYLOGENY OF THE SEXUALLY DECEPTIVE ORCHID GENUS OPHRYS L. (ORCHIDACEAE) CHAPTER 2: ANALYSIS OF VARIATION AND SPECIATION IN THE OPHRYS SPHEGODES SPECIES COMPLEX CHAPTER 3: FLORAL ISOLATION IS THE MAIN REPRODUCTIVE BARRIER AMONG CLOSELY RELATED SEXUALLY DECEPTIVE ORCHIDS CHAPTER 4: SPECIATION BY DISTURBANCE: A POPULATION STUDY OF CENTRAL ITALIAN OPHRYS SPHEGODES LINEAGES CONTRIBUTION OF CO-AUTHORS ACKNOWLEDGEMENTS 3 GENERAL INTRODUCTION ORCHIDS With more than 22.000 accepted species in 880 genera (Pridgeon et al. 1999), the family of the Orchidaceae is the largest family of angiosperm plants. Recently discovered fossils document their existence for at least 15 Ma. The last common ancestor of all orchids has been estimated to exist about 80 Ma ago (Ramirez et al. 2007, Gustafsson et al. 2010). Orchids are cosmopolitan, distributed on all continents and a great variety of habitats, ranging from deserts and swamps to arctic regions. Two large groups can be distinguished: Epiphytic and epilithic orchids attach themselves with aerial roots to trees or stones, mostly halfway between the ground and the upper canopy where they absorb water through the velamen of their roots. -
Phytogeographical Analysis and Ecological Factors of the Distribution of Orchidaceae Taxa in the Western Carpathians (Local Study)
plants Article Phytogeographical Analysis and Ecological Factors of the Distribution of Orchidaceae Taxa in the Western Carpathians (Local study) Lukáš Wittlinger and Lucia Petrikoviˇcová * Department of Geography and Regional Development, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 94974 Nitra, Slovakia; [email protected] * Correspondence: [email protected]; Tel.: +421-907-3441-04 Abstract: In the years 2018–2020, we carried out large-scale mapping in the Western Carpathians with a focus on determining the biodiversity of taxa of the family Orchidaceae using field biogeographical research. We evaluated the research using phytogeographic analysis with an emphasis on selected ecological environmental factors (substrate: ecological land unit value, soil reaction (pH), terrain: slope (◦), flow and hydrogeological productivity (m2.s−1) and average annual amounts of global radiation (kWh.m–2). A total of 19 species were found in the area, of which the majority were Cephalenthera longifolia, Cephalenthera damasonium and Anacamptis morio. Rare findings included Epipactis muelleri, Epipactis leptochila and Limodorum abortivum. We determined the ecological demands of the abiotic environment of individual species by means of a functional analysis of communities. The research confirmed that most of the orchids that were studied occurred in acidified, calcified and basophil locations. From the location of the distribution of individual populations, it is clear that they are generally arranged compactly and occasionally scattered, which results in ecological and environmental diversity. During the research, we identified 129 localities with the occurrence of Citation: Wittlinger, L.; Petrikoviˇcová, L. Phytogeographical Analysis and 19 species and subspecies of orchids. We identify the main factors that threaten them and propose Ecological Factors of the Distribution specific measures to protect vulnerable populations. -
Higher Seed Number Compensates for Lower Fruit Set in Deceptive Orchids
Journal of Ecology 2016, 104, 343–351 doi: 10.1111/1365-2745.12511 Higher seed number compensates for lower fruit set in deceptive orchids Judit Sonkoly1*, Anna E. Vojtko2,Jacint Tok€ olyi€ 3,Peter Tor€ ok€ 4,Gabor Sramko5,6, Zoltan Illyes 7 and Attila Molnar V.5 1Department of Ecology, University of Debrecen, H-4032, Debrecen, Hungary; 2Department of Tisza Research, Centre for Ecological Research, MTA, H-4026 Debrecen, Hungary; 3MTA-DE “Lendulet€ ” Behavioural Ecology Research Group, University of Debrecen, H-4032 Debrecen, Hungary; 4MTA-DE Biodiversity and Ecosystem Services Research Group, University of Debrecen, H-4032 Debrecen, Hungary; 5Department of Botany, University of Debrecen, H-4032 Debrecen, Hungary; 6MTA-ELTE-MTM Ecology Research Group, H-1117 Budapest, Hungary; and 7Mindszenty Youth House, H-8900, Zalaegerszeg-Botfa, Hungary Summary 1. Floral deception is widespread in orchids, with more than one-third of the species being polli- nated this way. The evolutionary success of deceptive orchids is puzzling, as species employing this strategy are thought to have low reproductive success (less flowers yielding fruits) because of low pollination rates. However, direct measurements of total seed production in orchids – which is a bet- ter measure of reproductive success – are scarce due to the extremely small size of their seeds. 2. Here, we quantified seed numbers in 1015 fruits belonging to 48 orchid species from the Pannon- ian ecoregion (central Europe) and obtained fruit set and thousand-seed weight data for these species from the literature. We used phylogenetic comparative methods to test the hypothesis that deceptive species should compensate for their lower fruit set by having either more flowers, larger seeds or more seeds in a fruit. -
Platanthera Chapmanii: Culture, Population Augmentation, and Mycorrhizal Associations
Platanthera chapmanii: culture, population augmentation, and mycorrhizal associations By Kirsten Poff, B.S. A Thesis In Plant and Soil Science Submitted to the Graduate Faculty of Texas Tech University in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE Approved Dr. Jyotsna Sharma Chair of Committee Dr. Scott Longing Dr. John Zak Dr. Mark Sheridan Dean of the Graduate School August, 2016 © 2016, Kirsten Poff Texas Tech University, Kirsten Poff, August 2016 ACKNOWLEDGEMENTS First I would like to thank my mentor and advisor, Dr. Jyotsna Sharma for all of her help and support. She has challenged and encouraged me throughout my program and the duration of this project. Thanks to her, I am light-years ahead of where I was two years ago. Texas Parks and Wildlife is also gratefully acknowledged for funding portions of this study. I also wish to express my gratitude to Dr. John Zak for his enthusiasm and for encouraging my love of microbes. I also gratefully thank Dr. Scott Longing for his advice, and constructive comments. I sincerely thank all three committee members for all the time and energy they have spent on me throughout the duration of my project. I gratefully acknowledge Dr. Jason Woodward for his encouragement and recommendations as well. I also acknowledge Dr. Cynthia McKenney and Mr. Russel Plowman for their support; I now have a passion for teaching, and a much better understanding of what it is like to teach college level courses. I want to also thank Mr. Robby Carlson for his time and technological assistance.