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The National Titolare Di Tute THE NATIONALUS009816080B2 TITOLARE DI TUTE (12 ) United States Patent ( 10 ) Patent No. : US 9 ,816 , 080 B2 Lu et al. ( 45 ) Date of Patent: Nov . 14 , 2017 ( 54 ) DELIVERY OF CASO VIA ( 52 ) U . S . CI. CPC . .. C12N 9 / 16 ( 2013 .01 ) ; CO7K 7 / 06 ARRDC1 -MEDIATED MICROVESICLES (2013 . 01) ; C07K 14 / 4718 (2013 .01 ) ; C12N (ARMMS ) 9 /22 ( 2013 .01 ) ; @( 71) Applicants : President and Fellows of Harvard ( Continued ) College, Cambridge , MA (US ) ; The (58 ) Field of Classification Search Board of Trustees of the Leland CPC . .. C12N 9 / 16 ; C12N 2810 /85 ; CO7K 14 /315 ; Stanford Junior University , Stanford , CO7K 14 /47 ; CO7K 2319/ 01 ; CA (US ) ( Continued ) (56 ) References Cited @( 72 ) Inventors : Quan Lu , Newton , MA (US ) ; Qiyu Wang , Boston , MA (US ) ; Stanley N . U . S . PATENT DOCUMENTS Cohen , Palo Alto , CA (US ) 7 ,479 ,573 B2 1 / 2009 Chu et al. (73 ) Assignees : President and Fellows of Harvard 8 ,680 ,069 B2 3 / 2014 de Fougerolles et al. College, Cambridge , MA (US ) ; The (Continued ) Board of Trustees of the Leland Stanford Junior University , Stanford , FOREIGN PATENT DOCUMENTS CA (US ) EP 2 604 255 Al 6 / 2013 WOWO 2011/ 127219 A1 10 / 2011 @( * ) Notice : Subject to any disclaimer , the term of this (Continued ) patent is extended or adjusted under 35 U . S . C . 154 ( b ) by 0 days. OTHER PUBLICATIONS (21 ) Appl. No. : 14 / 929 ,177 International Search Report and Written Opinion for PCT/ US2013 / 024839 , dated May 28 , 2013 . (22 ) Filed : Oct . 30 , 2015 (Continued ) (65 ) Prior Publication Data Primary Examiner — Jeffrey E Russel (74 ) Attorney, Agent, or Firm — Wolf, Greenfield & US 2016 /0206566 A1 Jul . 21, 2016 Sacks , P . C . ( 57 ) ABSTRACT Related U . S . Application Data Methods, systems, compositions and strategies for the deliv (60 ) Provisional application No .62 / 073 , 241, filed on Oct . ery of WW domain -containing fusion proteins into cells in vivo , ex vivo , or in vitro via ARMMsare provided .Methods , 31 , 2014 . systems, compositions and strategies for the delivery of (51 ) Int. Ci. Cas9 proteins and / or Cas9 variants into cells in vivo , ex CO7K 14 /47 ( 2006 . 01) vivo , or in vitro via fusion to ARMM associated proteins COZK 19 /00 (2006 .01 ) ( e . g . , ARRDC1 or TSG101 ) are also provided . ( Continued ) 17 Claims, 9 Drawing Sheets Interaction between the PPXY motif of [ 21 ??d th = } } } ??? ?f the PPXY } } } {{ Cas9 fu patel ? WW US 9 ,816 ,080 B2 Page 2 (51 ) Int. Cl. Genbank Submission ; NIH /NCBI , Accession No . NP _ 848495 . C12N 9 / 16 ( 2006 .01 ) Skarnes et al . , Feb . 26 , 2014 . C12N 15 / 87 Genbank Submission ; NIH /NCBI , Accession No . NP _ 006283 . ( 2006 .01 ) Rush et al ., May 4 , 2014 . C12N 9 / 22 ( 2006 . 01 ) Genbank Submission ; NIH /NCBI , Accession No . NP _ 068684. COZK 7706 ( 2006 . 01) Gunn et al ., Feb . 26 , 2014 . C12N 9 / 00 ( 2006 .01 ) Genbank Submission ; NIH /NCBI , Accession No . NP _ 853659. A61K 9 / 127 ( 2006 .01 ) Leithe et al. , Aug. 10 , 2014 . Genbank Submission ; NIH /NCBI , Accession No . NC _ 017053 . ( 52 ) U . S . CI. Fittipaldi et al ., Jul . 6 , 2013 . CPC . .. .. C12N 9 /93 (2013 . 01) ; A61K 9 /1272 Genbank Submission ; NIH /NCBI , Accession No. NC _ 015683. (2013 .01 ) ; COZK 2319 / 00 ( 2013 .01 ) ; COOK Trost et al ., Jul. 6 , 2013 . 2319 /01 ( 2013 .01 ) ; CO7K 2319 / 03 ( 2013 .01 ) ; Genbank Submission ; NIH /NCBI , Accession No . NC _ 017317 . CO7K 2319 / 70 ( 2013 .01 ) ; CO7K 2319 / 85 Trost et al ., Jun . 11 , 2013 . ( 2013 .01 ) ; C12N 2810 /85 ( 2013 .01 ) Genbank Submission ; NIH /NCBI , Accession No . NC _ 016782 . Trost et al ., Jun . 11 , 2013 . (58 ) Field of Classification Search Genbank Submission ; NIH / NCBI, Accession No . NC _ 016786 . CPC . .. .. CO7K 2319 / 03 ; C07K 2319 /035 ; C07K Trost et al. , Aug . 28 , 2013 . 2319 / 10 ; COOK 2319 / 85 ; CO7K 2319 /95 Genbank Submission ; NIH /NCBI , Accession No . NC _ 021284 . Ku See application file for complete search history . et al. , Jul. 12 , 2013 . Genbank Submission ; NIH /NCBI , Accession No. NC _ 017861 . (56 ) References Cited Heidelberg et al. , Jun . 11 , 2013 . Genbank Submission ; NIH /NCBI , Accession No. NC _ 021846 . Lo U . S . PATENT DOCUMENTS et al . , Jul . 22 , 2013 . Genbank Submission ; NIH /NCBI , Accession No . NC _ 021314 . 8 ,795 , 965 B2 * 8 / 2014 Zhang . .. .. .. .. C12N 9 /22 Zhang et al. , Jul . 15 , 2013 . 424 / 94 . 1 Genbank Submission ; NIH /NCBI , Accession No . NC _ 018010 . 2006 /0188560 Al 8 / 2006 Cheresh et al. Lucas et al ., Jun . 11 , 2013 . 2011/ 0053157 Al 3 / 2011 Skog et al . 2011/ 0151460 A1 6 / 2011 Klass et al. Genbank Submission ; NIH /NCBI , Accession No . NC _ 018721 . 2012 / 0129759 Al 5 / 2012 Liu et al. Feng et al ., Jun . 11, 2013 . 2014 / 0273226 AL 9 / 2014 Wu Genbank Submission ; NIH /NCBI , Accession No . YP _ 820832 . 2014 /0364588 A1 12 / 2014 Haugwitz et al. Makarova et al ., Aug . 27 , 2013 . 2015 / 0050699 AL 2 / 2015 Siksnys et al. Genbank Submission ; NIH /NCBI , Accession No . NP _ 472073 . 2015 /0071903 A1 * 3 / 2015 Liu A61K 38 /465 Glaser et al. , Jun . 27 , 2013 . 424 /94 . 3 Genbank Submission ; NIH /NCBI , Accession No . YP _ 002344900 . 2015 /0071906 A1 3 / 2015 Liu et al. Gundogdu et al ., Jul. 11, 2013 . 2015 /0118216 AL 4 /2015 Liu et al . Genbank Submission ; NIH /NCBI , Accession No . YP _ 002342100 . 2015 /0315252 A1 * 11/ 2015 Haugwitz .. .. .. .. .. C12P 21/ 02 Bernardini et al ., Jun . 10 , 2013 . 530 / 350 Genbank Submission ; NIH /NCBI , Accession No . NP _ 056092 . 2 . 2016 /0200779 AL 7 /2016 Liu et al . Wilkars et al. , Aug . 16 , 2014 . 3 pages. 2016 / 0201089 Al * 7 / 2016 Gersbach . .. .. .. .. C07K 14 /4708 Genbank Submission ; NIH /NCBI , Accession No . NM _ 015277 . 5 . 424 / 94 . 3 Wilkars et al ., Aug . 16 , 2014 . 9 pages . UniProt Submission ; UniProt, Accession No . Q9HOMO . Last FOREIGN PATENT DOCUMENTS modified Oct . 29 , 2014 , version 136 UniProt Submission ; UniProt , Accession No. 000308 . Last modi WO WO 2013 /013105 A2 1 / 2013 fied Oct. 29 , 2014 , version 141 . WO WO 2013 / 119602 AL 8 / 2013 UniProt Submission ; UniProt , Accession No . P46934 . Last modi WO WO 2014 /093655 A2 6 /2014 fied Oct. 29 , 2014 , version 152 . wo WO 2014 / 165825 A2 * 10 / 2014 UniProt Submission ; UniProt, Accession No . Q9HCE7 .Last modi fied Oct . 29 , 2014 , version 136 . OTHER PUBLICATIONS UniProt Submission ; UniProt , Accession No. Q9HAU4 . Last modi fied Oct. 29 , 2014 , version 143 . International Preliminary Report on Patentability for PCT/ US2013 / UniProt Submission ; UniProt, Accession No . Q96J02 . Last modi 024839 , dated Aug . 21 , 2014 . fied Oct. 29 , 2014 , version 129 . International Search Report and Written Opinion for PCT /US2014 / UniProt Submission ; UniProt, Accession No. Q76N89 . Last modi 054247 , dated Mar . 27 , 2015 . fied Oct . 29 , 2014 , version 95 . International Preliminary Report on Patentability for PCT /US2014 / UniProt Submission ; UniProt , Accession No . Q9P2P5 . Last modi 054247, dated Mar . 17 , 2016 . fied Oct. 29 , 2014 , version 105 . International Search Report and Written Opinion for PCT/ US2015 / Adrian et al. , Targeted Saint- O - Somes for improved intracellular 058479 , dated Feb . 11 , 2016 . delivery of siRNA and cytotoxic drugs into endothelial cells . J [No Author Listed ] , Invitrogen LipofectamineTM 2000 product Control Release . Jun . 15 , 2010 ; 144 ( 3 ) : 341 - 9 . doi: 10 . 1016 / j .jconrel . sheets , 2002 . 2 pages . 2010 .03 . 003 . Epub Mar . 11 , 2010 . [No Author Listed ] , Invitrogen LipofectamineTM 2000 product Aguilera et al. , Systemic in vivo distribution of activatable cell sheets , 2005 . 3 pages. penetrating peptides is superior to that of cell penetrating peptides. [No Author Listed ] , Invitrogen LipofectamineTM LTX product Integr Biol (Camb ) . Jun . 2009 ; 1 ( 5 - 6 ) :371 - 81 . doi: 10 . 1039 / sheets , 2011 . 4 pages . b904878b . Epub May 11, 2009 . [No Author Listed ], Thermo Fisher Scientific — How Cationic Lipid Allen et al. , Liposomal drug delivery systems: from concept to Mediated Transfection Works , retrieved from the internet Aug. 27 , clinical applications. Adv Drug Deliv Rev . Jan . 2013 ;65 ( 1 ) :36 -48 . 2015 . 2 pages . doi: 10 . 1016 / j .addr . 2012 .09 . 037 . Epub Oct . 1 , 2012 . Genbank Submission ; NIH /NCBI , Accession No . NP _ 689498 . Al- Taei et al ., Intracellular traffic and fate of protein transduction Puca et al ., Mar. 22 , 2014 . domains HIV - 1 TAT peptide and octaarginine . Implications for their Genbank Submission ; NIH /NCBI , Accession No . NP _ 001155957 . utilization as drug delivery vectors. Bioconjug Chem . Jan . -Feb . Skarnes et al ., Feb . 26 , 2014 . 2006 ; 17 ( 1 ) : 90 - 100 . US 9 ,816 ,080 B2 Page 3 ( 56 ) References Cited Freed et al. , The cell biology of HIV - 1 and other retroviruses . Retrovirology . Nov . 3 , 2006 ; 3 : 77 . OTHER PUBLICATIONS Fuchs et al. , Polyarginine as a multifunctional fusion tag. Protein Sci. Jun . 2005 ; 14 ( 6 ) : 1538 - 44 . Alvarez , On the origins of arrestin and rhodopsin . BMC Evol Biol. Fujii et al. , Beyond Tsg101: the role of Alix in ‘ ESCRTing ’ HIV - 1. Jul. 29 , 2008 ; 8 : 222 . doi: 10 . 1186 / 1471 -2148 - 8 -222 . Nat Rev Microbiol . Dec . 2007 ; 5 ( 12 ) :912 - 6 . Babst et al. , Mammalian tumor susceptibility gene 101 ( TSG101 ) Garrus et al ., Tsg101 and the vacuolar protein sorting pathway are and the yeast homologue , Vps23p , both function in late endosomal essential for HIV - 1 budding . Cell. Oct. 5 , 2001; 107 ( 1 ) :55 -65 . trafficking . Traffic . Mar. 2000 ; 1 ( 3 ) : 248 - 58 . Gordley et al. , Synthesis of programmable integrases. Proc Natl Babst , A protein ' s final ESCRT. Traffic . Jan . 2005 ;6 ( 1 ) : 2 - 9 . Bache et al. , Hrs regulates multivesicular body formation via Acad Sci USA . Mar. 31, 2009 ; 106 ( 13 ) :5053 - 8 . doi: 10 . 1073 /pnas . ESCRT recruitment to endosomes . J Cell Biol. Aug . 4 , 0812502106 . Epub Mar . 12 , 2009 . 2003 ; 162 ( 3 ) :435 - 42 . Gottlinger et al. , Effect of mutations affecting the pá gag protein on Basha et al.
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