1Dept. of Speech, Language, and Hearing Sciences, Boston Univ., Boston, MA; Neuromorphometrics, Inc. 2Neuromorphometrics, Inc., Somerville, MA Funded by SBIR grant R43 MH084358 from the National Institute of Mental Health

References for Brain Labeling Objective Digital Atlas To build a statistically meaningful, MRI-based interactive atlas of the human Probabilistic Atlases The modern atlas has multiple subjects with explicitly defined borders, regions cerebral sulci that represents an update and large-scale expansion of the atlas by Traditional Atlases and surfaces, is stereotaxic, searchable, expandable, and allows interaction Ono and colleagues (“Ono”; Thieme Medical, Stuttgart, 1990). and manipulation in the native space of individual scan.

Surface-Assisted Manual Labeling

Examples of Sulcal Variability Manually drawn “parcellation lines” visualized with the white matter surface highlight sulcal locations and configurations. Common Patterns of Cingulate :

Common Patterns: % Total % RH %LH 2D and 3D Visualization “Double Parallel” 48 30 65 Single, Continuous Segment 45 35 55 Two Segments 40 40 40 Three Segments 13 5 20 Intersects Superior Rostral Sulcus 55 65 45

NeuroMorphoNaut, open-source software developed by Neuromorphometrics, was used to manually label 20 unique T1 scans from the Open Access Series of Imaging Studies (OASIS) database. The slice-by-slice manually labeled white matter is used to generate a highly accurate white matter surface that assists in the identification of anatomical landmarks. Single cingulate (green), formed by a single continuous segment that intersect the superior rostral sulcus (SROS; blue) Cortical Labeling Protocol Database of Labeled Scans Database of Normative Values

“Double Parallel” with SROS intersection Two Segments, No SROS Intersection

Position of the Lateral Orbital Sulcus (LOS) relative to the Anterior Horizontal Ramus of the (AHLS): Future Directions aals anterior ascending ramus of the lateral sulcus hs Heschl's sulcus ots occipitotemporal sulcus ahls anterior horizontal ramus of the lateral sulcus ifrs pals posterior ascending ramus of the lateral sulcus • Label more brains and catalog more sulcal variants aocs anterior occipital sulcus ihs interhemispheric sulcus phls posterior horizontal ramus of the lateral sulcus cas callosal sulcus iros inferior rostral sulcus pis primary intermediate sulcus • Develop interactive atlas software ccs itps pocs postcentral sulcus cgs its pos parietooccipital sulcus • Develop automated means for sulcus identification, variant classification cis central insular sulcus lhos lateral H-shaped orbital sulcus prcs precentral sulcus cos collateral sulcus locs sbps subparietal sulcus • Customized brain classification based on “sulcal signatures” crs circular insular sulcus los lateral orbital sulcus sfrs cs ls lateral sulcus sros superior rostral sulcus • Optimize labeling protocol fms frontomarginal sulcus mho medial H-shaped orbital sulcus sts ftts first transverse temporal sulcus olfss thos transverse H-shaped orbital sulcus Reference 49 regions of interest per hemisphere rely on 36 sulci according to the LOS (tan) Medial to AHLS (green) in 35% LOS Lateral to AHLS in 65% of M. Ono, S. Kubik and C. Abernathey (1990). Atlas of the cerebral sulci. G. Thieme brainCOLOR labeling Protocol (http://www.braincolor.org/). of hemispheres (25% in LH, 45% in RH) hemispheres (55% in RH, 75% in LH) Verlag, Stuttgart, Germany