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Phylogenetic Structure Analysis Based on Blue Light

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Phylogenetic Structure Analysis Based on Blue Light Phylogenetic Structure Analysis Based on Blue Light Receptor Cryptochrome: Insights into How Light Shape the Vertical Structure of Forest Community Qiming Mei1, Ju-Yu Lian1, Yue Wang1, Meng Li1, Xujun Gui1, Zheng-Feng Wang1, Hong-Lin Cao1, and Wan-Hui Ye1 1South China Botanical Garden, Chinese Academy of Sciences October 13, 2020 Abstract Light-regime variability is an important environmental factor which shapes a forest community. So far, none focused on the phylogenetic pattern of plant light receptors, which reflects how genes' evolution influences the coexistence of species in a community. In this study, we analyzed community phylogenetic structure of the south subtropical forest by sequences of plant blue light receptor cryptochrome (CRY) and compared the results of DNA barcodes. Patterns of community assembly was estimated by net relatedness index (NRI) and nearest taxon index (NTI). We found that CRY showed quite different phylogenetic structure as compared to DNA barcoding results, all habitats displayed consistent phylogenetic structure patterns, suggesting a convergent evolution of light sensing system of plant in local adaptation. Also, both NRI and NTI values increased through the time, indicating that the phylogenetic structure of tree community became more overdispersion as succession proceeds; phylogenetic closely-related species tended to co-occur and environmental filtering played a more important role in the community assembly. Furthermore, phylogenetic patterns were more clustering in upper canopy layers, and NTI values of all canopy layers were above zero, suggesting that phylogenetically related species tended to coexist and adapted to similar light conditions. Hosted file Manuscript.pdf available at https://authorea.com/users/366771/articles/486413-phylogenetic- structure-analysis-based-on-blue-light-receptor-cryptochrome-insights-into-how-light- shape-the-vertical-structure-of-forest-community Posted on Authorea 13 Oct 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160257635.51966646/v1 | This a preprint and has not been peer reviewed. Data may be preliminary. 1 (a) (b) Habitat 500 400 Low valley Low slope 300 High valley Westing (m) Westing 200 Ridge High slope 100 0 0 100 200 300 400 Southing (m) 1.00 Myrtales Myrtales 1.00 Sapindales Sapindales 0.95 Malvales 1.00 1.00 Malvales 0.98 Oxalidales 1.00 Oxalidales 0.80 0.86 Malpighiales Malpighiales 0.98 1.00 Fabales Fagales 1.00 1.00 Fabales 0.64 Fagales 0.86 1.00 Rosales Rosales Saxifragales Saxifragales 1.00 Gentianales 0.65 Gentianales 0.97 Apiales Apiales 0.55 0.99 1.00 Aquifoliales Aquifoliales 1.00 0.90 1.00 Ericales Ericales 1.00 Proteales 0.62 Proteales Proteales Proteales Magnoliales Magnoliales 0.99 1.00 Laurales Laurales 1.00 Monocots Monocots 10 10 (a) (b) Posted on Authorea 13 Oct 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160257635.51966646/v1 | This a preprint and has not been peer reviewed. Data may be preliminary. 2 5.0 5.0 5.0 a b a,b a b a b b a c 2.5 2.5 2.5 b,c c b c b,c c NRI NRI c NRI c c b,c 0.0 0.0 0.0 −2.5 −2.5 −2.5 HG* HS LS* RT V* HG HS LS* RT V* HG* HS* LS* RT* V* Habitat Habitat Habitat (a) (b) (c) 3 c 3 3 b,c b b a b a b a a a,b a c a a,b a 2 2 a a a b 2 a a a a b 1 1 1 NTI NTI NTI 0 0 0 −1 −1 −1 Method −2 −2 −2 Barcode CRY HG* HS LS* RT V* HG* HS* LS* RT* V* HG* HS* LS* RT V* Habitat Habitat Habitat (d) (e) (f) 2 2 1 1 NTI NRI 0 0 −1 Method −1 Barcode 2005* 2010* 2015* 2005* 2010* 2015* Year Year CRY Posted on Authorea 13 Oct 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160257635.51966646/v1 | This a preprint and has not been peer reviewed. Data may be preliminary. 3 # # # # # # # # # # # 2.5 # 2.5 2.5 NRI NRI NRI 0.0 0.0 0.0 −2.5 −2.5 −2.5 ES* LS ES* LS* ES* LS* Succession Succession Succession (a) (b) (c) # # # # # # 2 2 2 0NTI 0NTI 0NTI −2 −2 −2 Method Barcode CRY ES* LS* ES* LS* ES* LS* Succession Succession Succession (d) (e) (f) Posted on Authorea 13 Oct 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160257635.51966646/v1 | This a preprint and has not been peer reviewed. Data may be preliminary. 4 a 4 4 b b a,b a,b a a,b b b a a a b b a c 2 2 NRI NRI 0 0 −2 −2 Sh* Un Ca Em* Sh* Un* Ca Em* Layer Layer (a) (b) b c b a 2 2 b b a b c c a b 0 0 NTI NTI −2 −2 −4 −4 Method Barcode Sh* Un* Ca* Em* Sh* Un* Ca* Em* CRY Layer Layer (c) (d) Posted on Authorea 13 Oct 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.160257635.51966646/v1 | This a preprint and has not been peer reviewed. Data may be preliminary. 5.
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