MAP3K10 (Human) Recombinant Protein

MAP3K10 (Human) Recombinant Gene Alias: MLK2, MST Protein Gene Summary: The protein encoded by this gene is a member of the serine/threonine kinase family. This Catalog Number: P5599 kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be Regulation Status: For research use only (RUO) phoshorylated, and thus activated by JNK kinases. This Product Description: Human MAP3K10 (NP_002437.2, kinase functions preferentially on the JNK signaling 75 a.a. - 462 a.a.) partial recombinant protein with GST pathway, and is reported to be involved in nerve growth tag expressed in baculovirus infected Sf21 cells. factor (NGF) induced neuronal apoptosis. [provided by RefSeq] Host: Insect Theoretical MW (kDa): 71 Applications: Func, SDS-PAGE (See our web site product page for detailed applications information) Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols Form: Liquid Preparation Method: Baculovirus infected insect cell (Sf21) expression system Purification: Glutathione sepharose chromatography Purity: 82 % by SDS-PAGE/CBB staining Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM. Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol) Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 4294 Gene Symbol: MAP3K10 Page 1/1 Powered by TCPDF (www.tcpdf.org).

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Advancing a Clinically Relevant Perspective of the Clonal Nature of Cancer
Advancing a clinically relevant perspective of the clonal nature of cancer Christian Ruiza,b, Elizabeth Lenkiewicza, Lisa Eversa, Tara Holleya, Alex Robesona, Jeffrey Kieferc, Michael J. Demeurea,d, Michael A. Hollingsworthe, Michael Shenf, Donna Prunkardf, Peter S. Rabinovitchf, Tobias Zellwegerg, Spyro Moussesc, Jeffrey M. Trenta,h, John D. Carpteni, Lukas Bubendorfb, Daniel Von Hoffa,d, and Michael T. Barretta,1 aClinical Translational Research Division, Translational Genomics Research Institute, Scottsdale, AZ 85259; bInstitute for Pathology, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; cGenetic Basis of Human Disease, Translational Genomics Research Institute, Phoenix, AZ 85004; dVirginia G. Piper Cancer Center, Scottsdale Healthcare, Scottsdale, AZ 85258; eEppley Institute for Research in Cancer and Allied Diseases, Nebraska Medical Center, Omaha, NE 68198; fDepartment of Pathology, University of Washington, Seattle, WA 98105; gDivision of Urology, St. Claraspital and University of Basel, 4058 Basel, Switzerland; hVan Andel Research Institute, Grand Rapids, MI 49503; and iIntegrated Cancer Genomics Division, Translational Genomics Research Institute, Phoenix, AZ 85004 Edited* by George F. Vande Woude, Van Andel Research Institute, Grand Rapids, MI, and approved June 10, 2011 (received for review March 11, 2011) Cancers frequently arise as a result of an acquired genomic insta- on the basis of morphology alone (8). Thus, the application of bility and the subsequent clonal evolution of neoplastic cells with puriﬁcation methods such as laser capture microdissection does variable patterns of genetic aberrations. Thus, the presence and not resolve the complexities of many samples. A second approach behaviors of distinct clonal populations in each patient’s tumor is to passage tumor biopsies in tissue culture or in xenografts (4, 9– may underlie multiple clinical phenotypes in cancers.
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PRODUCT SPECIFICATION Anti-MAP3K10 Product Datasheet
Anti-MAP3K10 Product Datasheet Polyclonal Antibody PRODUCT SPECIFICATION Product Name Anti-MAP3K10 Product Number HPA007039 Gene Description mitogen-activated protein kinase kinase kinase 10 Clonality Polyclonal Isotype IgG Host Rabbit Antigen Sequence Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence: LPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGG SSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEER LKGLGEGSKQWSSS Purification Method Affinity purified using the PrEST antigen as affinity ligand Verified Species Human Reactivity Recommended IHC (Immunohistochemistry) Applications - Antibody dilution: 1:200 - 1:500 - Retrieval method: HIER pH6 ICC-IF (Immunofluorescence) - Fixation/Permeabilization: PFA/Triton X-100 - Working concentration: 0.25-2 µg/ml Characterization Data Available at atlasantibodies.com/products/HPA007039 Buffer 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Concentration Lot dependent Storage Store at +4°C for short term storage. Long time storage is recommended at -20°C. Notes Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. For protocols, additional product information, such as images and references, see atlasantibodies.com. Product of Sweden. For research use only. Not intended for pharmaceutical development, diagnostic, therapeutic or any in vivo use. No products from Atlas Antibodies may be resold, modified for resale or used to manufacture commercial products without prior written approval from Atlas Antibodies AB. Warranty: The products supplied by Atlas Antibodies are warranted to meet stated product specifications and to conform to label descriptions when used and stored properly. Unless otherwise stated, this warranty is limited to one year from date of sales for products used, handled and stored according to Atlas Antibodies AB's instructions. Atlas Antibodies AB's sole liability is limited to replacement of the product or refund of the purchase price.
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Supplemental Material 1
Cdk20 Csrp2bp Bub1 Tmed3 1700012B15Rik Uck1 Atad2 Pbk Rfc4 Ucp3 Mtmr7 Bid Rad9 Hadh Zc3h12a Bcl2l11 1700052K11Rik Cd83 Ncaph Cdca7l Ezh2 Kif23 Rel Cdh13 Nr4a1 Pola1 Phf19 Vldlr Snx7 Fam96a Ripk2 Ccne2 Ncapd2 Pqlc3 A130009I22Rik Niacr1 Peli1 Cenpn Ralgds Dbf4 Trpc6 Gadd45b Dyrk2 Il1b Prim1 Pcna Dnmt1 Ttc30a1 Cflar Fas Tro Tank Rapgef2 Icosl 9930012K11Rik Cd28 Ccdc86 Ube2f Klhl6 Commd8 Ccrl2 D4Wsu53e Tnfaip8l2 Ehd1 Kdm2b Uhrf1 Trim37 Tecrl AI467606 Ilf2 Cobra1 Aurkb Cdc42ep2 Nfkbil2 Appbp2 Nfkbiz Zfp85-rs1 Nlrp3 Zmym5 Il6 Foxo3 Sept3 Ets2 Serpinb2 Smc3 Msh6 Itga5 Gpsm3 Slbp Dennd4c Rffl Trappc1 Mrpl34 Socs3 Ocel1 Tnfaip3 Swap70 1110003O08Rik Junb Pmm1 Cxcl10 Brca1 Acpl2 Coq10b Marcksl1 Map3k10 Braf Tnf Icam1 Pold3 AI462493 Nfkbia Dnmbp Dnajc9 Tnip1 Chfr Trim13 Slc35c2 Rb1 C78513 Limk1 C1qa Fen1 BC088983 Tsc22d3 Tnfsf9 Mapk3 E2f1 Zc3h12c Casp4 Ttf2 Maff Gmnn Zfp715 Mdm2 Mcm7 Irf1 Parp1 Ftsj2 Myo10 Irak2 Mid1ip1 Apeh Rad51c Map3k4 Tk1 E2f7 Tiparp Ptgs2 Spata13 Hmga2-ps1 Wdhd1 Clspn Ubac1 Erp29 Thap4 Dna2 Prkag2 Il1a Rfwd3 Gbp3 Cdca5 3110043O21Rik Clec4e Cxcl11 Speg Tarbp2 Cdk2 Zufsp Cdca2 E2f2 1700054N08Rik Tnfsf10 5730499H23Rik Inf2 C1qb Anapc5 Sco1 Bst2 2010107G23Rik Cnpy4 D10Wsu102e Brip1 H2-D1 Gpd1l Suv420h2 4930579G24Rik Cdc6 Cntnap2 Cyp2b10 Plod2 Irf7 C2 Lsm3 9330175E14Rik Aak1 Ick Rassf7 Cd22 Cd72 Tap1 Tcn2 Malt1 Uba7 Atp1a2 Ar Tap2 Cd86 Herc5 Heca Atp2a2 Ttc28 Dhrs3 Stat1 Ccdc22 Abca5 Nhlrc2 Ddx58 Gpx1 Dgkd Fbxl7 Lap3 Lama4 Neil1 Enpp4 Tpst1 Cybb Ccne1 Sec24d Fcgr4 Hk3 Rasgrp2 Dcun1d2 Vegfa Ecsit Ddx60 Eif2ak2 Cyp2r1 Gimap4 Fip1l1
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PRODUCTS and SERVICES Target List
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Supplementary Table 5.List of the 220 Most Frequently Amplified Genes In
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Clinical, Molecular, and Immune Analysis of Dabrafenib-Trametinib
Supplementary Online Content Chen G, McQuade JL, Panka DJ, et al. Clinical, molecular and immune analysis of dabrafenib-trametinib combination treatment for metastatic melanoma that progressed during BRAF inhibitor monotherapy: a phase 2 clinical trial. JAMA Oncology. Published online April 28, 2016. doi:10.1001/jamaoncol.2016.0509. eMethods. eReferences. eTable 1. Clinical efficacy eTable 2. Adverse events eTable 3. Correlation of baseline patient characteristics with treatment outcomes eTable 4. Patient responses and baseline IHC results eFigure 1. Kaplan-Meier analysis of overall survival eFigure 2. Correlation between IHC and RNAseq results eFigure 3. pPRAS40 expression and PFS eFigure 4. Baseline and treatment-induced changes in immune infiltrates eFigure 5. PD-L1 expression eTable 5. Nonsynonymous mutations detected by WES in baseline tumors This supplementary material has been provided by the authors to give readers additional information about their work. © 2016 American Medical Association. All rights reserved. Downloaded From: https://jamanetwork.com/ on 09/30/2021 eMethods Whole exome sequencing Whole exome capture libraries for both tumor and normal samples were constructed using 100ng genomic DNA input and following the protocol as described by Fisher et al.,3 with the following adapter modification: Illumina paired end adapters were replaced with palindromic forked adapters with unique 8 base index sequences embedded within the adapter. In-solution hybrid selection was performed using the Illumina Rapid Capture Exome enrichment kit with 38Mb target territory (29Mb baited). The targeted region includes 98.3% of the intervals in the Refseq exome database. Dual-indexed libraries were pooled into groups of up to 96 samples prior to hybridization.
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Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM 014911.2 KIAA1048, Dkfzp686k16132 AP2 Associated Kinase 1
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM_014911.2 KIAA1048, DKFZp686K16132 AP2 associated kinase 1 (AAK1) AATK NM_001080395.2 AATYK, AATYK1, KIAA0641, LMR1, LMTK1, p35BP apoptosis-associated tyrosine kinase (AATK) ABL1 NM_007313.2 ABL, JTK7, c-ABL, p150 v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1) ABL2 NM_007314.3 ABLL, ARG v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2) ACVR1 NM_001105.2 ACVRLK2, SKR1, ALK2, ACVR1A activin A receptor ACVR1B NM_004302.3 ACVRLK4, ALK4, SKR2, ActRIB activin A receptor, type IB (ACVR1B) ACVR1C NM_145259.2 ACVRLK7, ALK7 activin A receptor, type IC (ACVR1C) ACVR2A NM_001616.3 ACVR2, ACTRII activin A receptor ACVR2B NM_001106.2 ActR-IIB activin A receptor ACVRL1 NM_000020.1 ACVRLK1, ORW2, HHT2, ALK1, HHT activin A receptor type II-like 1 (ACVRL1) ADCK1 NM_020421.2 FLJ39600 aarF domain containing kinase 1 (ADCK1) ADCK2 NM_052853.3 MGC20727 aarF domain containing kinase 2 (ADCK2) ADCK3 NM_020247.3 CABC1, COQ8, SCAR9 chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1) ADCK4 NM_024876.3 aarF domain containing kinase 4 (ADCK4) ADCK5 NM_174922.3 FLJ35454 aarF domain containing kinase 5 (ADCK5) ADRBK1 NM_001619.2 GRK2, BARK1 adrenergic, beta, receptor kinase 1 (ADRBK1) ADRBK2 NM_005160.2 GRK3, BARK2 adrenergic, beta, receptor kinase 2 (ADRBK2) AKT1 NM_001014431.1 RAC, PKB, PRKBA, AKT v-akt murine thymoma viral oncogene homolog 1 (AKT1) AKT2 NM_001626.2 v-akt murine thymoma viral oncogene homolog 2 (AKT2) AKT3 NM_181690.1
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Mixed Lineage Kinases Activate MEK Independently of RAF to Mediate Resistance to RAF Inhibitors
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Mitogen Activated Protein Kinase (MAPK) Pathway Mutations and Drug Resistance
Author Manuscript Published OnlineFirst on February 13, 2013; DOI: 10.1158/1078-0432.CCR-12-0383 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Molecular Pathways: Mitogen Activated Protein Kinase (MAPK) Pathway Mutations and Drug Resistance Antonia L. Pritchard 1 and Nicholas K. Hayward 1* 1. Oncogenomics Research Group, CBCRC Building, Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, QLD 4006, Australia *Corresponding Author: Nicholas K. Hayward Oncogenomics Research Group, Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, QLD 4006, Australia [email protected] Conflict of Interest Statement: No relationship that could be construed as resulting in an actual, potential, or perceived conflict of interest are declared by either Dr. Pritchard or Prof. Hayward. Word counts: Abstract: 243 Background + Clinical translational advances: 3320 Number of References: 98 Downloaded from clincancerres.aacrjournals.org on October 6, 2021. © 2013 American Association for Cancer Research. Author Manuscript Published OnlineFirst on February 13, 2013; DOI: 10.1158/1078-0432.CCR-12-0383 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ABSTRACT Receptor tyrosine kinases are a diverse family of transmembrane proteins that can activate multiple pathways upon ligation of the receptor, one of which is the series of mitogen- activated protein kinase (MAPK) signalling cascades. The MAPK pathways play critical roles in a wide variety of cancer types, from haematological malignancies, to solid tumours. Aberrations include altered expression levels and activation states of pathway components, which can sometimes be attributable to mutations in individual members.
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The Role of the Hedgehog Pathway in Chemoresistance of Gastrointestinal Cancers
cells Review The Role of the Hedgehog Pathway in Chemoresistance of Gastrointestinal Cancers Yabing Liang 1, Ling Yang 1,* and Jingwu Xie 2,* 1 Inner Mongolia Key Laboratory of Medical Cell Biology, Clinical Medical Research Center of the Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010050, China; [email protected] 2 Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA * Correspondence: [email protected] (L.Y.); [email protected] (J.X.) Abstract: The hedgehog pathway, which plays a significant role in embryonic development and stem cell regulation, is activated in gastrointestinal cancers. Chemotherapy is widely used in cancer treatment. However, chemoresistance becomes a substantial obstacle in cancer therapy. This review focuses on the recent advances in the hedgehog pathway’s roles in drug resistance of gastrointestinal cancers and the novel drugs and strategies targeting hedgehog signaling. Keywords: the hedgehog pathway; chemotherapy; resistance; gastric cancer; colorectal cancer; pancreatic cancer 1. Introduction Citation: Liang, Y.; Yang, L.; Xie, J. The hedgehog (HH) pathway plays a crucial role in embryonic development, tissue The Role of the Hedgehog Pathway in homeostasis, and carcinogenesis [1,2]. HH ligands activate signaling by binding to receptor Chemoresistance of Gastrointestinal patched 1 homolog (PTCH1). In the absence of HH ligands, PTCH1 prevents smoothened Cancers. Cells 2021, 10, 2030. https:// (SMO) from transducing a signal to the downstream glioma-associated oncogene homolog doi.org/10.3390/cells10082030 (GLI) transcription factors. HH ligands bind to PTCH1, and relieve PTCH1’s inhibition on SMO, allowing SMO to signal downstream effectors GLI, which activates the target genes Academic Editors: Maria via specific genomic DNA sequences (TGGGTGGTC) [3,4].
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Kinome Expression Profiling to Target New Therapeutic Avenues in Multiple Myeloma
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