MAP3K10 (Human) Recombinant Gene Alias: MLK2, MST Protein Gene Summary: The protein encoded by this gene is a member of the serine/threonine kinase family. This Catalog Number: P5599 kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be Regulation Status: For research use only (RUO) phoshorylated, and thus activated by JNK kinases. This Product Description: Human MAP3K10 (NP_002437.2, kinase functions preferentially on the JNK signaling 75 a.a. - 462 a.a.) partial recombinant protein with GST pathway, and is reported to be involved in nerve growth tag expressed in baculovirus infected Sf21 cells. factor (NGF) induced neuronal apoptosis. [provided by RefSeq] Host: Insect Theoretical MW (kDa): 71 Applications: Func, SDS-PAGE (See our web site product page for detailed applications information) Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols Form: Liquid Preparation Method: Baculovirus infected insect cell (Sf21) expression system Purification: Glutathione sepharose chromatography Purity: 82 % by SDS-PAGE/CBB staining Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM. Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol) Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 4294 Gene Symbol: MAP3K10 Page 1/1 Powered by TCPDF (www.tcpdf.org).