CENPT (NM 025082) Human 3' UTR Clone – SC200995 | Origene

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CENPT (NM 025082) Human 3' UTR Clone – SC200995 | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for SC200995 CENPT (NM_025082) Human 3' UTR Clone Product data: Product Type: 3' UTR Clones Product Name: CENPT (NM_025082) Human 3' UTR Clone Vector: pMirTarget (PS100062) Symbol: CENPT Synonyms: C16orf56; CENP-T; SSMGA ACCN: NM_025082 Insert Size: 140 bp Insert Sequence: >SC200995 3’UTR clone of NM_025082 The sequence shown below is from the reference sequence of NM_025082. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC AGTGGCAACTCTGTCTTCCCTGCCCAGTAGTGGCCAGGCTTCAACACTTTCCCTGTCCCCACCTGGGGA CTCTTGCCCCCACATATTTCTCCAGGTCTCCTCCCCACCCCCCCAGCATCAATAAAGTGTCATAAACAG AA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG Restriction Sites: SgfI-MluI OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). RefSeq: NM_025082.4 Summary: The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008] Locus ID: 80152 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 CENPT (NM_025082) Human 3' UTR Clone – SC200995 MW: 4.9 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Gene Knockdown of CENPA Reduces Sphere Forming Ability and Stemness of Glioblastoma Initiating Cells
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Supplemental Information Supplemental Fig. S1. CENPT Is
Supplemental information Supplemental Fig. S1. CENPT is not recovered under native conditions. N- ChIP-seq merged pairs for CENPA, CENPT, H3 and Input (top, from GEO GSE60951 (Henikoff et al. 2015) and paired-end reads for 3 biological replicates of CENPT (bottom) were mapped to the indicated 340-bp α-satellite dimers. Each column of four profiles is scaled to the maximum peak. The relative scale is the area of the CENPA, CENPC or H3 profile (top) or the CENPT1, CENPT2 or CENPT3 profile (bottom) divided by the area of the D5Z1 profile, where the numbers reflect the product of the total sequence abundance times the enrichment. Supplemental Fig. S2. CENPA, CENP- C and CENPT are cross-linked to same α-satellites. The X-ChIP profiles of CENPT with two different antibodies (Abcam and Bethyl) on centromeric α-satellites are highly similar to each other and to those of CENPA and CENPC. Supplemental Fig. S3. Fragment ends from X-ChIP (top four panels) and native bottom panel) datasets were mapped to the Cen1-like consensus and DXZ1 sequences. The CENPB box region is indicated by the magenta box. Supplemental Fig. S4. Fragment length distribution of Sequential ChIP datasets on Cen1-like α-satellites. A 100 100 90 90 80 80 70 70 60 Chr1_7340 60 Chr1_11321 50 Chr1_17109 50 40 ChrX_7340 40 ChrX_10470 30 ChrX_17119 30 20 ChrX_11321 20 10 10 0 % of total centromere α-satellite 0 100 95 90 85 80 75 70 65 60 % iden6ty threshold B DNA sequence divergence CENPA nucleosomes H3 nucleosomes CENPA occupancy CENPA Domain CENPA domains H3 OR H3 CENPA CENPA Supplemental Fig.
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CENPT Bridges Adjacent CENPA Nucleosomes on Young Human Α-Satellite Dimers
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