Metagenomics Revealing Molecular Proling of Microbial Community Structure and Metabolic Capacity In Bamucuo, Tibet Cai Wei Shanghai Ocean University Dan Sun Shanghai Ocean University Wenliang Yuan Jiaxing University Lei Li Fudan University Chaoxu Dai Shanghai Ocean University Zuozhou Chen Shanghai Ocean University Xiaomin Zeng Central South University Xiangya Public Health School Shihang Wang Shanghai Ocean University Yifan Tang Hunan Normal University School of Medicine Shouwen Jiang Shanghai Ocean University Zhichao Wu Shanghai Ocean University Xiaoning Peng Hunan Normal University School of Medicine Linhua Jiang Fudan University Sihua peng (
[email protected] ) Shanghai Ocean University https://orcid.org/0000-0001-7231-666X Research Page 1/36 Keywords: shotgun metagenomics, microbial community, extreme environment, Tibet, Qinghai-Tibet Plateau Posted Date: May 24th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-505014/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 2/36 Abstract Background: The Qinghai-Tibet Plateau (QTP) is the highest plateau in the world, and the microorganisms there play vital ecological roles in the global biogeochemical cycle; however, detailed information on the microbial communities in QTP is still lacking. Results: Here, we performed a landscape survey of the microorganisms in Bamucuo, Tibet, resulting in 160,212 (soil) and 135,994 (water) contigs by shotgun metagenomic methods, and generated 75 nearly complete metagenome-assembled genomes (MAGs). Proteobacteria, Actinobacteria and Firmicutes were found to be the three most dominant bacterial phyla, while Euryarchaeota was the most dominant archaeal phylum. Surprisingly, Pandoravirus salinus was found in the soil microbial community.