NTML 384-Well Array Well Identification
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METACYC ID Description A0AR23 GO:0004842 (Ubiquitin-Protein Ligase
Electronic Supplementary Material (ESI) for Integrative Biology This journal is © The Royal Society of Chemistry 2012 Heat Stress Responsive Zostera marina Genes, Southern Population (α=0. -
Product Information
Product Information Betaine Aldehyde (chloride) Item No. 17270 CAS Registry No.: 7758-31-8 Formal Name: N,N,N-trimethyl-2-oxo- ethanaminium, monochloride MF: C5H12NO • Cl H FW: 137.6 • Cl- Purity: N+ ≥95% O Stability: ≥2 years at -20°C Supplied as: A crystalline solid Laboratory Procedures For long term storage, we suggest that betaine aldehyde (chloride) be stored as supplied at -20°C. It should be stable for at least two years. Betaine aldehyde (chloride) is supplied as a crystalline solid. A stock solution may be made by dissolving the betaine aldehyde (chloride) in the solvent of choice. Betaine aldehyde (chloride) is soluble in the organic solvents DMSO, which should be purged with an inert gas, at a concentration of approximately 1 mg/ml. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of betaine aldehyde (chloride) can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of betaine aldehyde (chloride) in PBS, pH 7.2, is approximately 5 mg/ml. We do not recommend storing the aqueous solution for more than one day. Betaine aldehyde is the physiological intermediate in the oxidation of choline to betaine. This step is involved in glycine, serine, and threonine metabolism. Related Products For a list of related products please visit: www.caymanchem.com/catalog/17270 Cayman Chemical Mailing address 1180 E. -
Phosphatidylinositol-3-Kinase in Tomato (Solanum Lycopersicum. L) Fruit and Its Role in Ethylene Signal Transduction and Senescence
Phosphatidylinositol-3-Kinase in Tomato (Solanum lycopersicum. L) Fruit and Its Role in Ethylene Signal Transduction and Senescence by Mohd Sabri Pak Dek A Thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Doctor of Philosophy in Plant Agriculture Guelph, Ontario, Canada © Mohd Sabri Pak Dek,June, 2015 ABSTRACT PHOSPHATIDYLINOSITOL-3-KINASE IN TOMATO (SOLANUM LYCOPERSICUM. L) FRUIT AND ITS ROLE IN ETHYLENE SIGNAL TRANSDUCTION AND SENESCENCE Mohd Sabri Pak Dek Co-Advisors: University of Guelph, 2015 Professor G. Paliyath Professor J. Subramanian The ripening process is initiated by ethylene through a signal transduction cascade leads to the expression of ripening-related genes and catabolism of membrane, cell wall, and storage components. One of the minor components in membrane phospholipids is phosphatidylinositol (PI). Phosphatidylinositol-3-kinase (PIK) is an enzyme that phosphorylates PI at the 3-OH position of inositol head group to produce phosphatidylinositol 3-phosphate (PI3P). Phosphorylation of PI may be an early event in the ethylene signal transduction pathway that generates negatively charged domains on the plasma membrane. PI3P domains may potentially serve as a docking site for phospholipase D (PLD) after ethylene stimulation. It is hypothesized that ethylene stimulation may activate PI3K resulting in enhanced level of phosphorylated phosphatidylinositol. However, the properties and function of PI3K is not well understood in plants. In the present study, the effect of PI3K inhibition during tomato fruit ripening was evaluated. This study demonstrated that PI3K activity is required for normal ripening process of the fruit. Inhibition of PI3K activity using wortmannin significantly reduced tomato ripening process. -
The Genome of Nanoarchaeum Equitans: Insights Into Early Archaeal Evolution and Derived Parasitism
The genome of Nanoarchaeum equitans: Insights into early archaeal evolution and derived parasitism Elizabeth Waters†‡, Michael J. Hohn§, Ivan Ahel¶, David E. Graham††, Mark D. Adams‡‡, Mary Barnstead‡‡, Karen Y. Beeson‡‡, Lisa Bibbs†, Randall Bolanos‡‡, Martin Keller†, Keith Kretz†, Xiaoying Lin‡‡, Eric Mathur†, Jingwei Ni‡‡, Mircea Podar†, Toby Richardson†, Granger G. Sutton‡‡, Melvin Simon†, Dieter So¨ ll¶§§¶¶, Karl O. Stetter†§¶¶, Jay M. Short†, and Michiel Noordewier†¶¶ †Diversa Corporation, 4955 Directors Place, San Diego, CA 92121; ‡Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182; §Lehrstuhl fu¨r Mikrobiologie und Archaeenzentrum, Universita¨t Regensburg, Universita¨tsstrasse 31, D-93053 Regensburg, Germany; ‡‡Celera Genomics Rockville, 45 West Gude Drive, Rockville, MD 20850; Departments of ¶Molecular Biophysics and Biochemistry and §§Chemistry, Yale University, New Haven, CT 06520-8114; and ʈDepartment of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 Communicated by Carl R. Woese, University of Illinois at Urbana–Champaign, Urbana, IL, August 21, 2003 (received for review July 22, 2003) The hyperthermophile Nanoarchaeum equitans is an obligate sym- (6–8). Genomic DNA was either digested with restriction en- biont growing in coculture with the crenarchaeon Ignicoccus. zymes or sheared to provide clonable fragments. Two plasmid Ribosomal protein and rRNA-based phylogenies place its branching libraries were made by subcloning randomly sheared fragments point early in the archaeal lineage, representing the new archaeal of this DNA into a high-copy number vector (Ϸ2.8 kbp library) kingdom Nanoarchaeota. The N. equitans genome (490,885 base or low-copy number vector (Ϸ6.3 kbp library). DNA sequence pairs) encodes the machinery for information processing and was obtained from both ends of plasmid inserts to create repair, but lacks genes for lipid, cofactor, amino acid, or nucleotide ‘‘mate-pairs,’’ pairs of reads from single clones that should be biosyntheses. -
Structure-Function Analysis of the Catalytic Domain of the Histidine Kinase Chea
Loyola University Chicago Loyola eCommons Dissertations Theses and Dissertations 1997 Structure-Function Analysis of the Catalytic Domain of the Histidine Kinase Chea Dolph David Ellefson Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_diss Part of the Microbiology Commons Recommended Citation Ellefson, Dolph David, "Structure-Function Analysis of the Catalytic Domain of the Histidine Kinase Chea" (1997). Dissertations. 3425. https://ecommons.luc.edu/luc_diss/3425 This Dissertation is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Dissertations by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1997 Dolph David Ellefson LOYOLA UNIVERSITY MEDICAL CENTER LIBRARY LOYOLA UNIVERSITY OF CHICAGO STRUCTURE-FUNCTION ANALYSIS OF THE CATALYTIC DOMAIN OF THE HISTIDINE KINASE CHEA A DISSERTATION SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL IN CANDIDACY FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY BY DOLPH DAVID ELLEFSON CHICAGO, ILLINOIS MAY, 1997 Copyright by Dolph David Ellefson, 1997 All Rights Reserved ii ACKNOWLEDGEMENTS I would like to thank my director, Dr. Alan J. Wolfe, for his support, advice, and encouragment during the many years in his laboratory. In his laboratory, I was given a rare opportunity to explore a new arena of science and interact with a field of gifted researchers who I would not known otherwise. I would also like to thank the members of my committee, Ors. -
Genetic Expression Profile Analysis of the Temporal Inhibition of Quercetin and Naringenin on Lactobacillus Rhamnosus GG
robioti f P cs o & l a H n e r a u l t o h J Liu, et al., J Prob Health 2016, 4:2 Journal of Probiotics & Health DOI: 10.4172/2329-8901.1000139 ISSN: 2329-8901 Research Article Open Access Genetic Expression Profile Analysis of the Temporal Inhibition of Quercetin and Naringenin on Lactobacillus Rhamnosus GG Linshu Liu1*, Jenni Firrman1, Gustavo Arango Argoty2, Peggy Tomasula1, Masuko Kobori3, Liqing Zhang2 and Weidong Xiao4* 1Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA 2Virginia Tech College of Engineering, Department of Computer Science, 1425 S Main St. Blacksburg, VA 24061, USA 3National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan 4*Department of Microbiology and Immunology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, USA *Corresponding author: Weidong Xiao, Department of Microbiology and Immunology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, USA, Tel: 215-707-6392; E-mail: [email protected], LinShu Liu, Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA. E-mail: [email protected] Received date: Jan 29, 2015; Accepted date: Feb 15, 2016; Published date: Feb 22, 2016 Copyright: © 2016 Liu LS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. -
Generate Metabolic Map Poster
Authors: Pallavi Subhraveti Ron Caspi Peter Midford Peter D Karp An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Ingrid Keseler Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. Gcf_001591825Cyc: Bacillus vietnamensis NBRC 101237 Cellular Overview Connections between pathways are omitted for legibility. Anamika Kothari sn-glycerol phosphate phosphate pro phosphate phosphate phosphate thiamine molybdate D-xylose D-ribose glutathione 3-phosphate D-mannitol L-cystine L-djenkolate lanthionine α,β-trehalose phosphate phosphate [+ 3 more] α,α-trehalose predicted predicted ABC ABC FliY ThiT XylF RbsB RS10935 UgpC TreP PutP RS10200 PstB PstB RS10385 RS03335 RS20030 RS19075 transporter transporter of molybdate of phosphate α,β-trehalose 6-phosphate L-cystine D-xylose D-ribose sn-glycerol D-mannitol phosphate phosphate thiamine glutathione α α phosphate phosphate phosphate phosphate L-djenkolate 3-phosphate , -trehalose 6-phosphate pro 1-phosphate lanthionine molybdate phosphate [+ 3 more] Metabolic Regulator Amino Acid Degradation Amine and Polyamine Biosynthesis Macromolecule Modification tRNA-uridine 2-thiolation Degradation ATP biosynthesis a mature peptidoglycan a nascent β an N-terminal- -
Mullergliaregnerationtranscriptome
gene.id fc1 fc2 fc3 fc4 p1 p2 p3 p4 FDR.pvalue-1 Gene Symbol Gene Title Pathway go biological process term go molecular function term go cellular component term Dr.10016.1.A1_at -2.33397 -3.86923 -4.38335 -2.39965 0.935201 0.320614 0.208 0.917227 0.208 zgc:77556 zgc:77556 --- proteolysis arylesterase activity /// metallopeptidase activity /// zinc ion --- binding Dr.10024.1.A1 at 1.483417 2.531269 2.089091 1.698761 1 0.613 0.998961 1 0.613 zgc:171808 zgc:171808--- cell-cell signaling --- --- Dr.10051.1.A1 at -1.78449 -2.22024 -1.70922 -1.99464 1 0.901663 1 0.999955 0.9017 ccng2 cyclin G2 --- --- --- --- Dr.10061.1.A1 at 2.065955 2.274632 2.248958 2.507754 0.992 0.718655 0.83 0.600609 0.6006 zgc:173506 zgc:173506--- --- --- --- Dr.10061.2.A1 at 2.131883 2.616483 2.49378 2.815337 0.983443 0.711513 0.805599 0.519115 0.5191 zgc:173506 Zgc:17350 --- --- --- --- Dr.10065.1.A1 at -1.02315 -2.01596 -2.29343 -1.88944 1 0.999955 0.957199 1 0.9572 zgc:114139 zgc:114139--- --- --- cytoplasm /// centrosome Dr.10070.1.A1_at -1.74365 -2.52206 -2.39093 -1.86817 1 0.741254 0.885401 1 0.7413 fbp1a fructose-1, --- carbohydrate metabolic process hydrolase activity /// phosphoric ester hydrolase activity --- Dr.10074.1.S1_at 6.035545 10.44051 7.880519 5.020371 0.1104 0.044 0.062491 0.144679 0.044 pdgfaa platelet-der--- multicellular organismal development /// cell proliferation growth factor activity membrane Dr.10095.1.A1 at -1.73408 -2.11615 -1.47234 -2.19919 1 0.997562 1 0.978177 0.9782 wu:fk95g04 wu:fk95g04--- --- --- --- Dr.10110.1.S1 a at 3.929761 5.798708 -
1/05661 1 Al
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date _ . ... - 12 May 2011 (12.05.2011) W 2 11/05661 1 Al (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/00 (2006.0 1) C12Q 1/48 (2006.0 1) kind of national protection available): AE, AG, AL, AM, C12Q 1/42 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, (21) Number: International Application DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US20 10/054171 HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, (22) International Filing Date: KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, 26 October 2010 (26.10.2010) ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, (25) Filing Language: English SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, (26) Publication Language: English TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 61/255,068 26 October 2009 (26.10.2009) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, (71) Applicant (for all designated States except US): ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, MYREXIS, INC. -
Discovery of a Choline-Responsive Transcriptional Regulator in Burkholderia Xenovorans
Journal of Molecular Biology Research; Vol. 3, No. 1; 2013 ISSN 1925-430X E-ISSN 1925-4318 Published by Canadian Center of Science and Education Discovery of a Choline-Responsive Transcriptional Regulator in Burkholderia xenovorans Ricardo Martí-Arbona1, Tuhin S. Maity1, John M. Dunbar1, Clifford J. Unkefer1 & Pat J. Unkefer1 1 Los Alamos National Laboratory, Los Alamos, NM 87545, United States Correspondence: Ricardo Martí-Arbona, Los Alamos National Laboratory, Los Alamos, NM 87545, United States. E-mail: [email protected] Received: August 8, 2013 Accepted: August 20, 2013 Online Published: August 22, 2013 doi:10.5539/jmbr.v3n1p91 URL: http://dx.doi.org/10.5539/jmbr.v3n1p91 Abstract The search for effectors of novel transcriptional regulators is a challenging task. Here, we present the prediction and validation of an effector for a novel transcriptional regulator (TR). The clustering of genes around the gene coding for Bxe_A0425, a TR in Burkholderia xenovorans LB400 and its closest orthologs, suggests the conservation of a functional operon composed a several open reading frames from which a TR, a transporter, and two oxidoreductases can be easily identified. A search of operons containing these functional components revealed a remarkable resemblance of this system to the evolutionarily convergent and functionally conserved operons found in Escherichia coli, Bacillus subtilis, Staphylococcus xylosus and Pseudomonas aeruginosa. These operons are involved in the uptake and catabolism of choline to create the potent osmo-protectant molecule glycine betaine. We used frontal affinity chromatography coupled to mass spectrometry to screen for the binding of choline and other intermediates of the glycine biosynthesis pathway to the TR Bxe_0425. -
Metabolism and Occurrence of Methanogenic and Sulfate-Reducing Syntrophic Acetate Oxidizing Communities in Haloalkaline Environments
Delft University of Technology Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments Timmers, Peer H.A.; Vavourakis, Charlotte D.; Kleerebezem, Robbert; Sinninghe Damsté, Jaap S.; Muyzer, Gerard; Stams, Alfons J.M.; Sorokin, Dimity Y.; Plugge, Caroline M. DOI 10.3389/fmicb.2018.03039 Publication date 2018 Document Version Final published version Published in Frontiers in Microbiology Citation (APA) Timmers, P. H. A., Vavourakis, C. D., Kleerebezem, R., Sinninghe Damsté, J. S., Muyzer, G., Stams, A. J. M., Sorokin, D. Y., & Plugge, C. M. (2018). Metabolism and occurrence of methanogenic and sulfate- reducing syntrophic acetate oxidizing communities in haloalkaline environments. Frontiers in Microbiology, 9(DEC), [3039]. https://doi.org/10.3389/fmicb.2018.03039 Important note To cite this publication, please use the final published version (if applicable). Please check the document version above. Copyright Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policy Please contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. This work is downloaded from Delft University of Technology. For technical reasons the number of authors shown on this cover page is limited to a maximum of 10. fmicb-09-03039 December 6, 2018 Time: 15:4 # 1 ORIGINAL RESEARCH published: 10 December 2018 doi: 10.3389/fmicb.2018.03039 Metabolism and Occurrence of Methanogenic and Sulfate-Reducing Syntrophic Acetate Oxidizing Communities in Haloalkaline Environments Peer H. -
Supporting Information High-Throughput Virtual Screening
Supporting Information High-Throughput Virtual Screening of Proteins using GRID Molecular Interaction Fields Simone Sciabola, Robert V. Stanton, James E. Mills, Maria M. Flocco, Massimo Baroni, Gabriele Cruciani, Francesca Perruccio and Jonathan S. Mason Contents Table S1 S2-S21 Figure S1 S22 * To whom correspondence should be addressed: Simone Sciabola, Pfizer Research Technology Center, Cambridge, 02139 MA, USA Phone: +1-617-551-3327; Fax: +1-617-551-3117; E-mail: [email protected] S1 Table S1. Description of the 990 proteins used as decoy for the Protein Virtual Screening analysis. PDB ID Protein family Molecule Res. (Å) 1n24 ISOMERASE (+)-BORNYL DIPHOSPHATE SYNTHASE 2.3 1g4h HYDROLASE 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE 1.8 1cel HYDROLASE(O-GLYCOSYL) 1,4-BETA-D-GLUCAN CELLOBIOHYDROLASE I 1.8 1vyf TRANSPORT PROTEIN 14 KDA FATTY ACID BINDING PROTEIN 1.85 1o9f PROTEIN-BINDING 14-3-3-LIKE PROTEIN C 2.7 1t1s OXIDOREDUCTASE 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE 2.4 1t1r OXIDOREDUCTASE 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE 2.3 1q0q OXIDOREDUCTASE 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE 1.9 1jcy LYASE 2-DEHYDRO-3-DEOXYPHOSPHOOCTONATE ALDOLASE 1.9 1fww LYASE 2-DEHYDRO-3-DEOXYPHOSPHOOCTONATE ALDOLASE 1.85 1uk7 HYDROLASE 2-HYDROXY-6-OXO-7-METHYLOCTA-2,4-DIENOATE 1.7 1v11 OXIDOREDUCTASE 2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT 1.95 1x7w OXIDOREDUCTASE 2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT 1.73 1d0l TRANSFERASE 35KD SOLUBLE LYTIC TRANSGLYCOSYLASE 1.97 2bt4 LYASE 3-DEHYDROQUINATE DEHYDRATASE