Generic Pest Risk Analysis: Import of Transgenic Rice
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PCR-RFLP and Sequencing Analysis of Ribosomal DNA of Bursaphelenchus Nematodes Related to Pine Wilt Disease(L)
Fundam. appl. Nemalol., 1998,21 (6), 655-666 PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease(l) Hideaki IvVAHORI, Kaku TSUDA, Natsumi KANZAKl, Katsura IZUI and Kazuyoshi FUTAI Cmduate School ofAgriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Accepted for publication 23 December 1997. Summary -A polymerase chain reaction - restriction fragment polymorphism (PCR-RFLP) analysis was used for the discri mination of isolates of Bursaphelenchus nematode. The isolares of B. xylophilus examined originared from Japan, the United Stares, China, and Canada and the B. mucronatus isolates from Japan, China, and France. Ribosomal DNA containing the 5.8S gene, the internai transcribed spacer region 1 and 2, and partial regions of 18S and 28S gene were amplified by PCR. Digestion of the amplified products of each nematode isolate with twelve restriction endonucleases and examination of resulting RFLP data by cluster analysis revealed a significant gap between B. xylophllus and B. mucronatus. Among the B. xylophilus isolares examined, Japanese pathogenic, Chinese and US isolates were ail identical, whereas Japanese non-pathogenic isolares were slightly distinct and Canadian isolates formed a separate cluster. Among the B. mucronalUS isolates, two Japanese isolares were very similar to each other and another Japanèse and one Chinese isolare were identical to each other. The DNA sequence data revealed 98 differences (nucleotide substitutions or gaps) in 884 bp investigated between B. xylophilus isolare and B. mucronmus isolate; DNA sequence data of Aphelenchus avenae and Aphelenchoides fragariae differed not only from those of Bursaphelenchus nematodes, but also from each other. -
Prioritising Plant-Parasitic Nematode Species Biosecurity Risks Using Self Organising Maps
Prioritising plant-parasitic nematode species biosecurity risks using self organising maps Sunil K. Singh, Dean R. Paini, Gavin J. Ash & Mike Hodda Biological Invasions ISSN 1387-3547 Volume 16 Number 7 Biol Invasions (2014) 16:1515-1530 DOI 10.1007/s10530-013-0588-7 1 23 Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media Dordrecht. This e-offprint is for personal use only and shall not be self- archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com”. 1 23 Author's personal copy Biol Invasions (2014) 16:1515–1530 DOI 10.1007/s10530-013-0588-7 ORIGINAL PAPER Prioritising plant-parasitic nematode species biosecurity risks using self organising maps Sunil K. Singh • Dean R. Paini • Gavin J. Ash • Mike Hodda Received: 25 June 2013 / Accepted: 12 November 2013 / Published online: 17 November 2013 Ó Springer Science+Business Media Dordrecht 2013 Abstract The biosecurity risks from many plant- North and Central America, Europe and the Pacific parasitic nematode (PPN) species are poorly known with very similar PPN assemblages to Australia as a and remain a major challenge for identifying poten- whole. -
E PLANTAS: Fundamentos E Importância
NEMATOLOGIA DE PLANTAS: fundamentos e importância ii NEMATOLOGIA DE PLANTAS: fundamentos e importância Organizado por Luiz Carlos C. Barbosa Ferraz Docente aposentado da Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brasil Derek John F inlay Brown Pesquisador aposentado do Scottish Crop Research Institute (SCRI), atual James Hutton Institute, Dundee, Escócia uma publicação da iii Sociedade Brasileira de Nematologia / SBN Sede atual: Universidade Estadual do Norte Fluminense Darcy Ribeiro / CCTA. Av. Alberto Lamego, 2000 – Parque California 28013-602 – Campos dos Goytacazes (RJ) – Brasil E-mail: [email protected] Telefone: (22) 3012-4821 Site: http://nematologia.com.br © Sociedade Brasileira de Nematologia 2016. Todos os direitos reservados. É vedada a reprodução desta publicação, ou de suas partes, na forma impressa ou por outros meios, sem prévia autorização do representante legal da SBN. A sua utilização poderá vir a ocorrer estritamente para fins didáticos, em caráter eventual e com a citação da fonte. Ficha catalográfica elaborada por Marilene de Sena e Silva - CRB/AM Nº 561 F368 n FERRAZ, L.C.C.B.; BROWN, D.J.F. Nematologia de plantas: fundamentos e importância. L.C.C.B. Ferraz e D.J.F. Brown (Orgs.). Manaus: NORMA EDITORA, 2016. 251 p. Il. ISBN: 978-85-99031-26-1 2. Nematologia. 2. Doenças de plantas. 3. Vermes. I. Ferraz & Brown. II. Título. CDD: 632 iv Para Maria Teresa, Alex e Thais, que souberam entender a minha irresistível atração pela Nematologia e aceitar as muitas horas de plena dedicação a ela. In memoriam Alexandre M. Cintra Goulart, Anário Jaehn, Dimitry Tihohod, José Julio da Ponte, Luiz G. -
First Morphological and Molecular Report of Aphelenchoides Blastophthorus on Strawberry Plants in Switzerland
Plant Disease • XXXX • XXX:X-X • https://doi.org/10.1094/PDIS-07-18-1241-RE First Morphological and Molecular Report of Aphelenchoides blastophthorus on Strawberry Plants in Switzerland Erika Consoli,1 Andrea C. Ruthes,2 Eder Reinhard,1 and Paul Dahlin1,† 1 Agroscope, Research Division Plant Protection, Phytopathology and Zoology in Fruit and Vegetable Production, Wadenswil,¨ Switzerland 2 Independent Researcher, Wadenswil,¨ Switzerland Abstract Foliar nematodes represent a minor feeding group within the genus Aphe- barcoding of 18S ribosomal RNA (18S), 28S ribosomal RNA (28S), lenchoides Fischer, 1894. The facultative plant parasitic species A. blas- and cytochrome c oxidase I (COI) gene fragment analyses. Phylogenetic tophthorus can cause crinkling of leaves, reduced vigor, and stunting of analysis of 18S indicated that A. blastophthorus was grouped within agricultural and ornamental plants. Here we report the first finding of A. close distance to A. fragariae, a well-known foliar nematode affecting blastophthorus in leaves, crowns, and roots of strawberry plants collected strawberry plants. Furthermore, the newly generated molecular barcodes in Switzerland in 2018. Species identification was confirmed by morpho- of the partial 28S and COI of A. blastophthorus will support species iden- logical and morphometric characterization supported by molecular tification in the future. The genus Aphelenchoides is found worldwide and is commonly 2015). Symptoms attributable to the presence of A. blastophthorus known to comprise fungal-feeding nematodes (Hunt 1993). None- in Norwegian strawberries were reported in 1972, 1987, and 2000 theless, a few Aphelenchoides spp. are known to also feed on but investigations documented a negative effect on strawberry leaves, sprouts, or buds of plants (Hunt 1993; Sanchez-Monge´ yields and runner production only in 2000 (Haukeland and Brekke et al. -
Nematology Training Manual
NIESA Training Manual NEMATOLOGY TRAINING MANUAL FUNDED BY NIESA and UNIVERSITY OF NAIROBI, CROP PROTECTION DEPARTMENT CONTRIBUTORS: J. Kimenju, Z. Sibanda, H. Talwana and W. Wanjohi 1 NIESA Training Manual CHAPTER 1 TECHNIQUES FOR NEMATODE DIAGNOSIS AND HANDLING Herbert A. L. Talwana Department of Crop Science, Makerere University P. O. Box 7062, Kampala Uganda Section Objectives Going through this section will enrich you with skill to be able to: diagnose nematode problems in the field considering all aspects involved in sampling, extraction and counting of nematodes from soil and plant parts, make permanent mounts, set up and maintain nematode cultures, design experimental set-ups for tests with nematodes Section Content sampling and quantification of nematodes extraction methods for plant-parasitic nematodes, free-living nematodes from soil and plant parts mounting of nematodes, drawing and measuring of nematodes, preparation of nematode inoculum and culturing nematodes, set-up of tests for research with plant-parasitic nematodes, A. Nematode sampling Unlike some pests and diseases, nematodes cannot be monitored by observation in the field. Nematodes must be extracted for microscopic examination in the laboratory. Nematodes can be collected by sampling soil and plant materials. There is no problem in finding nematodes, but getting the species and numbers you want may be trickier. In general, natural and undisturbed habitats will yield greater diversity and more slow-growing nematode species, while temporary and/or disturbed habitats will yield fewer and fast- multiplying species. Sampling considerations Getting nematodes in a sample that truly represent the underlying population at a given time requires due attention to sample size and depth, time and pattern of sampling, and handling and storage of samples. -
Classification of Plant Diseases
K. K. WAGH COLLEGE OF AGRICULTURE, NASHIK DEPARTMENT OF PLANT PATHOLOGY THEORY NOTES Course No.: - PATH -121 Course Title: - Fundamentals of Plant Pathology Credits: - 3 (2+1) Compiled By Prof. Patil K.P. Assistant Professor Department of Plant Pathology Teaching Schedule a) Theory Lecture Topic Weightage (%) 1 Importance of plant diseases, scope and objectives of Plant 3 Pathology..... 2 History of Plant Pathology with special reference to Indian work 3 3,4 Terms and concepts in Plant Pathology, Pathogenesis 6 5 classification of plant diseases 5 6,7, 8 Causes of Plant Disease Biotic (fungi, bacteria, fastidious 10 vesicular bacteria, Phytoplasmas, spiroplasmas, viruses, viroids, algae, protozoa, and nematodes ) and abiotic causes with examples of diseases caused by them 9 Study of phanerogamic plant parasites. 3 10, 11 Symptoms of plant diseases 6 12,13, Fungi: general characters, definition of fungus, somatic structures, 7 14 types of fungal thalli, fungal tissues, modifications of thallus, 15 Reproduction in fungi (asexual and sexual). 4 16, 17 Nomenclature, Binomial system of nomenclature, rules of 6 nomenclature, 18, 19 Classification of fungi. Key to divisions, sub-divisions, orders and 6 classes. 20, 21, Bacteria and mollicutes: general morphological characters. Basic 8 22 methods of classification and reproduction in bacteria 23,24, Viruses: nature, architecture, multiplication and transmission 7 25 26, 27 Nematodes: General morphology and reproduction, classification 6 of nematode Symptoms and nature of damage caused by plant nematodes (Heterodera, Meloidogyne, Anguina etc.) 28, 29, Principles and methods of plant disease management. 6 30 31, 32, Nature, chemical combination, classification of fungicides and 7 33 antibiotics. -
JOURNAL of NEMATOLOGY Description of Aphelenchoides Giblindavisi N
JOURNAL OF NEMATOLOGY Article | DOI: 10.21307/jofnem-2018-035 Issue 3 | Vol. 50 (2018) Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination Farzad Aliramaji,1 Ebrahim Pourjam,1 Sergio Álvarez-Ortega,2 Farahnaz Jahanshahi Afshar,1,3 and Majid Abstract 1, Pedram * One new and one known species of the genus Aphelenchoides 1Department of Plant Pathology, from Iran are studied. Aphelenchoides giblindavisi n. sp. is mainly Faculty of Agriculture, Tarbiat characterized by having five lines in the lateral fields at mid-body, Modares University, Tehran, Iran. and a single mucro with several tiny nodular protuberances, giving 2 a warty appearance to it, as revealed by detailed scanning electron Departamento de Biología y microscopic (SEM) studies. The new species is further characterized Geología, Física y Química by having a body length of 546 to 795 μ m in females and 523 to 679 μ m Inorgánica, Universidad Rey Juan in males, rounded lip region separated from the rest body by a shallow Carlos, Campus de Móstoles, depression, 10 to 11 μm long stylet with small basal swellings, its 28933-Madrid, España. conus shorter than the shaft (m = 36–43), 52 to 69 µm long postvulval 3Iranian Research Institute of Plant uterine sac (PUS), males with 16 to 18 μ m long arcuate spicules, and Protection, Agricultural Research, three pairs of caudal papillae. The new species was morphologically Education and Extension compared with two species of the genus having five lines in the lateral Organization (AREEO), Tehran, Iran. fields namely A. paramonovi and A. shamimi and species having a warty-surfaced mucro at tail end and similar morphometric data *E-mail: [email protected]. -
Table of Contents
TABLE OF CONTENTS SESSION ONE – PLENARY SESSION ............................................................................................... 1 CHAIRS: MICHAEL HODDA & DAVID CHITWOOD Is Nematology a Jigsaw, a Tapestry or a Strange Attractor? 1 Hodda, M. Metagenomics, Big Science, and the Reformation of Nematology 2 Powers, T. A Practical Future for Nematology in the Real World 2 Nicol, J. & R. Sikora SESSION TWO – ECOLOGY AND BIODIVERSITY OF SOIL NEMATODES IN SUSTAINABLE SOIL CONSERVATION ............................................................................................ 3 CONVENORS: GREGOR YEATES & NIGEL BELL Nematode Assemblages and Soil Properties Are Closely Linked 3 Sánchez-Moreno, S. & H. Ferris Nematode Diversity and Function in Dutch Sand Dunes 4 Brinkman, E.P., H. Duyts & W.H. Van der Putten Nematode Diversity under Commercial Banana Production 5 Pattison, A., J. Cobon, M. Araya, L. Pocasangre, F. Rosales & R. Sikora How Different or Similar are Nematode Communities in Paddy and Upland Rice Fields 6 Okada, H., W. Abe, M. Komatsuzaki & M. Hiroki A Perspective on Diversity within Nematode Feeding Groups across Ecosystems 7 Yeates, G.W. SESSION THREE – MUTUALISTIC/PHORETIC ASSOCIATIONS AND INVERTEBRATE PARASITIC NEMATODES ............................................................................................................... 8 CONVENORS: ROBIN GIBLIN-DAVIS & KERRIE DAVIES Entomophilic Nematodes for Predictions of Worldwide Nematode Species Diversity 8 Giblin-Davis, R.M., N. Kanzaki & K.A. Davies Host Specificity, -
Collecting and Preserving Nematodes
Contents A SAFRINET MANUAL FOR NEMATOLOGY Collecting and Preserving Nematodes Compiled by the Biosystematics Division, ARC–PPRI, South Africa Sponsored by SDC, Switzerland Contents Collecting and Preserving Nematodes A Manual for Nematology by SAFRINET, the Southern African (SADC) LOOP of BioNET-INTERNATIONAL Compiled by the National Collection of Nematodes Biosystematics Division ARC – Plant Protection Research Institute Pretoria, South Africa Edited by K.P.N. Kleynhans Sponsored by The Swiss Agency for Development and Cooperation (SDC) Contents © 1999 ARC — Plant Protection Research Institute Private Bag X134, Pretoria, 0001 South Africa ISBN 0-620-23566-7 No part of this publication may be reproduced in any form or by any means, including photocopying and recording, without prior permission from the publisher. Layout, design, technical editing & production Isteg Scientific Publications, Irene Imageset by Future Graphics, Centurion Printed by Ultra Litho (Pty) Ltd, Heriotdale, Johannesburg Contents Preface This manual is a guide to a course in practical plant nematology for technical assistants of the SADC countries of the SAFRINET-loop of BioNET-INTERNATIONAL. The course is presented by the staff of the National Collection of Nematodes of the Plant Protection Research Institute and comprises lectures, discussions and practical sessions, aimed at teaching students to recognise the major groups of plant nematodes and the plant damage symptoms that they produce. Also included are techniques to collect, process and prepare nematodes for study and to preserve them in reference collections. The manual contains information on basic handling procedures, plant nematode morphology, and naming and classifying nematodes. A list of pertinent literature is provided. Contents Acknowledgements • Sincere thanks are due to Ms C. -
A4163 - Waller - Plant - All.Vp Monday, October 29, 2001 4:37:45 PM Color Profile: Disabled Composite Default Screen
Color profile: Disabled Composite Default screen Plant Pathologist’s Pocketbook 3rd Edition 1 Z:\Customer\CABI\A4084 - Waller - Plant Pathologists Pocketbook\A4163 - Waller - Plant - All.vp Monday, October 29, 2001 4:37:45 PM Color profile: Disabled Composite Default screen 2 Z:\Customer\CABI\A4084 - Waller - Plant Pathologists Pocketbook\A4163 - Waller - Plant - All.vp Monday, October 29, 2001 4:37:45 PM Color profile: Disabled Composite Default screen Plant Pathologist’s Pocketbook 3rd Edition Edited by J.M. Waller, J.M. Lenné and S.J. Waller CABI Publishing 3 Z:\Customer\CABI\A4084 - Waller - Plant Pathologists Pocketbook\A4163 - Waller - Plant - All.vp Monday, October 29, 2001 4:37:46 PM Color profile: Disabled Composite Default screen CABI Publishing is a division of CAB International CABI Publishing CABI Publishing CAB International 10 E 40th Street Wallingford Suite 3203 Oxon OX10 8DE New York, NY 10016 UK USA Tel: +44 (0)1491 832111 Tel: +1 212 481 7018 Fax: +44 (0)1491 833508 Fax: +1 212 686 7993 Email: [email protected] Email: [email protected] Web site: www.cabi-publishing.org ©CAB International 2002. All rights reserved. No part of this publication may be reproduced in any form or by any means, electronically, mechanically, by photocopying, recording or otherwise, without the prior permission of the copyright owners. A catalogue record for this book is available from the British Library, London, UK. Library of Congress Cataloging-in-Publication Data Plant pathologist’s pocketbook / edited by J.M. Waller, J.M. Lenné and S.J. Waller. --3rd ed. p. cm. Includes bibliographical references (p. -
DP 17: Aphelenchoides Besseyi, A. Fragariae and A. Ritzemabosi
ISPM 27 27 ANNEX 17 ENG DP 17: Aphelenchoides besseyi, A. fragariae and A. ritzemabosi INTERNATIONAL STANDARD FOR PHYTOSANITARY MEASURES PHYTOSANITARY FOR STANDARD INTERNATIONAL DIAGNOSTIC PROTOCOLS Produced by the Secretariat of the International Plant Protection Convention (IPPC) This page is intentionally left blank This diagnostic protocol was adopted by the Standards Committee on behalf of the Commission on Phytosanitary Measures in August 2016. The annex is a prescriptive part of ISPM 27. ISPM 27 Diagnostic protocols for regulated pests DP 17: Aphelenchoides besseyi, A. fragariae and A. ritzemabosi Adopted 2016; published 2016 CONTENTS 1. Pest Information ............................................................................................................................... 3 2. Taxonomic Information .................................................................................................................... 4 3. Detection ........................................................................................................................................... 5 3.1 Symptoms produced by the nematodes on host plants ...................................................... 5 3.1.1 Symptoms of Aphelenchoides besseyi ............................................................................... 5 3.1.2 Symptoms of Aphelenchoides fragariae ........................................................................... 5 3.1.3 Symptoms of Aphelenchoides ritzemabosi....................................................................... -
Nemat494abs 477..541
Journal of Nematology 49(4):477–541. 2017. Ó The Society of Nematologists 2017. ABSTRACTS CHEMICAL DEHYDRATION OF NEMATODES FOR SCANNING ELECTRON MICROSCOPY OBSERVATIONS. Abdel-Rahman, Fawzia H. Department of Biology, Texas Southern University. Houston, TX 77004. Scanning Electron Microscopy (SEM) of nematodes has become an essential component of new species description; however, the general preparation procedures for SEM can frequently result in specimen distortion during fixation and drying procedures. Here, a chemical preparation method using hexamethyldisilazane (HMDS) was employed to dry nematodes for SEM. The plant parasitic nematode Globodera sp., and the soil free-living nematode Caenorhabditis elegans were dried without any deformities. Cyst stages of Globodera, and the synchronized C. elegans hermaphrodites were used in this test. Globodera cysts were obtained in FAA, and the synchronized C. elegans hermaphrodites were killed with gentile heat, and fixed in FAA for 48 hours. All nematode specimens were ultrasonicated for few minutes and dehydrated through an acetone series, 25, 50, 75, 95%, and 3 changes of 100% acetone (30 minutes for each change). Nematodes then were transferred gradually to 100% hexamethyldisilazane (HMDS). This was done by transferring the acetone dehydrated nematodes gradually through a series of mixtures of acetone and HMDS to 100% HMDS (75% acetone and 25% HMDS, then 50% acetone and 50% HMDS, then 25% acetone and 75% HMDS, and finally 3 changes of 100% HMDS). All nematode specimens in 100% HMDS were left over night under the hood to dry. After the evaporation of HMDS, nematode specimens were observed with the stereomicroscope; dried nematodes were mounted on SEM aluminum stubs, sputter coated with gold and viewed with SEM using accelerated voltage of 15 KV.