Product datasheet Anti-TRAP alpha/TRAPA/SSR1 Antibody Catalog Number: A06993-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-TRAP alpha/TRAPA/SSR1 Antibody Name SSR1 Source Rabbit IgG Species Reactivity human, mouse, rat, monkey Tested Application WB, IHC-P, ICC/IF, FCM, Direct ELISA Contents 500 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human SSR1 recombinant (Position: E53-E286). Purification Immunogen affinity purified. Observed MW 32KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Translocon-associated protein subunit alpha is a protein that in humans is encoded by the SSR1 gene. The signal sequence receptor (SSR) is a glycosylated (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative . This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-TRAP alpha/TRAPA/SSR1 Antibody Catalog Number: A06993-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis analysis of anti-TRAP using anti-TRAP alpha/TRAPA/SSR1 alpha/TRAPA/SSR1 Antibody (A06993-1). The Antibody (A06993-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing mouse brain tissue tissue. conditions. Biotinylated goat anti- Lane 1: A431 whole cell rabbit IgG was used as lysates, secondary antibody. The Lane 2: HL-60 whole cell tissue section was lysates, developed using Strepavi Lane 3: T47D whole cell din-Biotin-Complex lysates, (SABC) (Catalog # Lane 4: HepG2 whole cell SA1022) with DAB as the lysates, chromogen. Lane 5: COS-7 whole cell lysates, Lane 6: HEK293 whole cell lysates, Lane 7: K562 whole cell lysates, Lane 8: U937 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat stomach tissue lysates, Lane 11: PC-12 whole cell lysates, Lane 12: mouse liver tissue lysates, Lane 13: mouse stomach tissue lysates, Use rabbit anti-SSR1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SSR1 at approximately 32KD. The expected band size for SSR1 is at 32KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-TRAP alpha/TRAPA/SSR1 Antibody Catalog Number: A06993-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Figure 3. IHC analysis Figure 4. IHC analysis using anti-TRAP using anti-TRAP alpha/TRAPA/SSR1 alpha/TRAPA/SSR1 Antibody (A06993-1). Antibody (A06993-1). detected in paraffin- detected in paraffin- embedded section of rat embedded section of brain tissue tissue. human liver cancer tissue. Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody. The secondary antibody. The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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