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USDA ARS National Clonal Germplasm Repository for Citrus and Dates, Riverside

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USDA ARS National Clonal Germplasm Repository for Citrus and Dates, Riverside USDAUSDA ARSARS NationalNational ClonalClonal GermplasmGermplasm RepositoryRepository forfor CitrusCitrus andand DatesDates ReportReport onon NCGRCDNCGRCD ActivitiesActivities JuneJune 20082008 ChallengesChallenges andand OpportunitiesOpportunities MissionMission Access,Access, evaluate,evaluate, maintain,maintain, preserve,preserve, andand distributedistribute germplasmgermplasm ofof CitrusCitrus,, CitrusCitrus relatives,relatives, andand datedate palmspalms MaintainMaintain informationalinformational filesfiles onon accessionsaccessions ConductConduct researchresearch thatthat isis compatiblecompatible withwith primaryprimary missionmission PersonnelPersonnel RichardRichard LeeLee Research Leader, Research Plant Pathologist (Perm) RobertRobert KruegerKrueger Curator, Horticulturalist (Perm) ManjunathManjunath KeremaneKeremane Post Doc (Temp) PersonnelPersonnel Polly Balance Biological Technician, Lab (Perm) Vicki Newman Biological Technician, Plants (Perm) Jaclyn Sweet (Resigned in June) Biological Technician, Plants (Perm) Vicki and Jaclyn Lee Gross John Lehr (Started in June) Biological Technician, Plants (Temp) Leila Ruiz (.75 FTE) Office Assistant (Temp) Lee Gross (.4 FTE) Maintenance Mechanic Polly Leila PersonnelPersonnel ThroughThrough UCR:UCR: ChandrikaChandrika Ramadugu,Ramadugu, VisitingVisiting AssistantAssistant ResearchResearch onon SCASCA withwith M.M. RooseRoose PartPart timetime studentsstudents Chandrika HiredHired throughthrough UCRUCR AgOpsAgOps 2.52.5 FTEFTE PersonnelPersonnel NancyNancy Knap,Knap, LAOLAO JoAnnJoAnn Moore,Moore, LocationLocation PurchasingPurchasing OfficerOfficer PeggyPeggy Carroll,Carroll, LocationLocation AccountantAccountant PersonnelPersonnel changeschanges PatriciaPatricia NielsenNielsen Retired,Retired, movedmoved toto NewNew YorkYork StateState BenBen RangelRangel 44 yearyear eligibilityeligibility endedended inin MayMay 07,07, positionposition willwill notnot bebe refilledrefilled JaclynJaclyn SweetSweet ResignedResigned toto gogo intointo businessbusiness withwith herher husbandhusband whowho justjust completedcompleted aa MBAMBA degreedegree GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections Citrus variety Collection (CVC) Belongs to UCR GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections CitrusCitrus varietyvariety CollectionCollection (CVC)(CVC) Belongs to UCR (Dr. T. Kahn) Used cooperatively by NCGRCD for characterization/evaluation, as a seed source, and occasionally material for distribution (pollen, flowers, leaves, etc; seed source trees tested for CLBV) SCA with UCR for maintenance and evaluation Over 1,010 accessions Endowment campaign underway by UCR to provide long term preservation of CVC GermplasmGermplasm collectioncollection CitrusCitrus varietyvariety CollectionCollection (CVC)(CVC) 139139 totaltotal treestrees plantedplanted inin CVCCVC inin 20072007 Includes 27 new accessions Includes 10 citrus relatives Remainder are replants, many due to the construction relocation GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections ProtectedProtected collectioncollection (PC)(PC) Small potted trees propagated from pathogen tested budwood Maintained in protected conditions in the screenhouse about 425 accessions New accessions are quarantined by USDA-APHIS and CDFA officials at the Repository, released to PC only after therapy and pathogen testing GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections ProtectedProtected collectioncollection (PC)(PC) Pathogen testing CTV ELISA annually Tested for DMV/CLBV Tested for leprosis testing for viroids and HLB via PCR About 40 being re-indexed for psorosis and for viroids (5 year schedule is goal) GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections CitrusCitrus relativesrelatives collectioncollection MaintainedMaintained inin greenhousesgreenhouses ifif extremelyextremely coldcold sensitivesensitive FieldField plantingplanting inin thethe moremore moderatemoderate coastalcoastal temperaturestemperatures nearnear Irvine,Irvine, somesome alsoalso inin thethe CoachellaCoachella ValleyValley inin thethe lowlow desertdesert climateclimate 8080 accessionsaccessions ofof generagenera otherother thanthan CitrusCitrus representingrepresenting 2828 ofof 3333 generagenera ofof AurantioideaeAurantioideae family,family, howeverhowever somesome generagenera representedrepresented byby onlyonly oneone accessionaccession GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections CitrusCitrus relativesrelatives collectioncollection 1818 treestrees plantedplanted atat SouthSouth CoastCoast FieldField StationStation 2 new accessions Field 45 is being removed, accessions are being reestablished into Field 25. Accessions remaining in Field 45 are those we commonly get seed requests for. GermplasmGermplasm collectioncollection CitrusCitrus relativesrelatives collectioncollection CoachellaCoachella ValleyValley AgriculturalAgricultural ResearchResearch StationStation (CVARS)(CVARS) Citrus relatives Most lost in January 07 freeze Replanted 40 trees Total Citrus relatives accessions: 22 Citrons wild or semi wild citrons, some have no flesh similar to Yemeni type and as such are of interest to Rabbis Trifoliate selections: 20 GermplasmGermplasm collectioncollection CitrusCitrus relativesrelatives collectioncollection CoachellaCoachella ValleyValley AgriculturalAgricultural ResearchResearch StationStation (CVARS)(CVARS) Seedling populations (monoembryonic & zygotics) 36 total accessions, 177 total trees Persian lime SPB-7 Wood pocket-free budline? Determine performance in warm climate GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections DateDate PalmPalm collectioncollection Coachella Valley Agricultural Research Center CVARS (most recently collection) Brawley (Imperial Valley Agricultural Research Station) GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections Date Palm collection Brawley—trees need clean up. Currently under contract to clean up trees and remove debris from ground. GermplasmGermplasm collectioncollection AA collectioncollection ofof collectionscollections DateDate PalmPalm collectioncollection 128 accessions total, includes some related species About 500 trees at CVARS, about 100 trees at Brawley CDFA permit received which allowed accessing 10 new varieties from Arizona Received 6 seed populations from Baja California from exploration trip by R. Krueger in Oct 2007 AccessionsAccessions NCGRCDNCGRCD recognizedrecognized asas aa quarantinequarantine facility,facility, cancan receivereceive accessionsaccessions directlydirectly fromfrom nationalnational andand internationalinternational sourcessources OneOne ofof threethree recognizedrecognized quarantinequarantine facilitiesfacilities forfor CitrusCitrus introductionsintroductions NCGRCDNCGRCD CitrusCitrus ClonalClonal ProtectionProtection Program,Program, CACA CitrusCitrus IntroductionIntroduction Program,Program, FLFL DistributionsDistributions 1400 1200 1000 800 600 400 200 0 2003 2004 2005 2006 2007 911 distributions in 2007 total of 90 different orders NewNew ProjectProject PlanPlan ApprovedApproved SpecificSpecific projectproject objectivesobjectives StrategicallyStrategically expandexpand andand improveimprove collectionscollections ofof prioritypriority geneticgenetic resourcesresources ofof citruscitrus andand datedate palmpalm andand associatedassociated informationinformation ConserveConserve andand propagatepropagate citruscitrus andand datedate geneticgenetic resourcesresources efficientlyefficiently andand effectively,effectively, andand distributedistribute pathogenpathogen--testedtested samplessamples andand associatedassociated informationinformation worldwideworldwide NewNew ProjectProject PlanPlan ApprovedApproved SpecificSpecific projectproject objectivesobjectives StrategicallyStrategically characterizecharacterize ((““genotypegenotype””)) andand evaluateevaluate ((““phenotypephenotype””)) citruscitrus andand datedate palmpalm geneticgenetic resourcesresources forfor prioritypriority geneticgenetic andand horticulturalhorticultural traitstraits DevelopDevelop moremore rapidrapid andand accurateaccurate diagnosticdiagnostic methodsmethods forfor prioritypriority graftgraft--transmissibletransmissible pathogenspathogens ofof citruscitrus toto promotepromote exchangeexchange ofof pathogenpathogen--testedtested stockstock andand efficientlyefficiently screenscreen forfor hosthost--plantplant resistanceresistance DatabasesDatabases DatabasesDatabases maintainedmaintained onon http://www.arshttp://www.ars-- grin.gov/rivgrin.gov/riv LocalLocal databasesdatabases havehave beenbeen migratedmigrated toto AccessAccess LookingLooking forwardforward toto improvementsimprovements inin GRINGRIN-- GlobalGlobal DatabasesDatabases GRINGRIN Most “permanent” accessions (CRC numbers) now loaded Many “non-permanent” accessions are loaded Ones not loaded are lacking critical information Date palms are up to date Improved information delivery When possible, provide improved passport data Improved readability by incorporating HTML coding Link to CVC, CCPP websites, other resources when available Older entries (1980s) need improvement SpecificSpecific CooperativeCooperative
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