Hyperion Imaging System A comprehensive solution for high-parameter tissue imaging
The Hyperion™ Imaging System is a transformative imaging solution Highlights that enables comprehensive analysis of cellular phenotypes and their interrelationships using Imaging Mass Cytometry™. Bringing Comprehensive—Highly together high-parameter CyTOF® technology with imaging capability, multiplexed IHC enables the Hyperion Imaging System enables you to visualize 4 to 37 simultaneous analysis of 4 to protein markers in the spatial context of the tissue microenvironment, 37 protein markers. surpassing the capabilities of fluorescent immunohistochemistry (IHC). With the ability to utilize up to 135 channels to detect additional Contextual—Get subcellular parameters, the Hyperion Imaging System is ideal to meet your resolution while preserving needs today and well into the future. tissue architecture and The Hyperion Imaging System is accompanied by a pathologist- cellular morphology. verified Maxpar® imaging antibody portfolio, a software suite for image acquisition and data analysis, and experienced Fluidigm Powerful—Preserve precious service and support. Delivering a comprehensive view from one samples and reduce scan, the Hyperion Imaging System can enable you to deeply profile variability by eliminating the precious FFPE or frozen tissues at subcellular resolution to power dependency on serial your next breakthrough. sections.
The Imaging Mass Cytometry workflow
1 2 3 4 DESIGN STAIN IMAGE ANALYZE panels using tissues (FFPE or frozen) protein markers at images in minutes pathologist-verified or fixed cells using subcellular resolution using the MCD Viewer Maxpar antibodies familiar IHC protocols. using the Hyperion and easily export for conjugated to Imaging System. secondary analysis. metal tags.
Figure 1. The Imaging Mass Cytometry workflow. A simple four-step workflow to deliver highly multiplexed imaging. Simplify multiplex panel design with pathologist-verified Maxpar antibodies Metal-tagged pathologist-verified Maxpar antibodies for Imaging Mass Cytometry are designed and optimized for use with the Hyperion Imaging System. These antibodies can be combined using a protocol that provides a common antigen retrieval step to simplify panel design for use with formalin-fixed, paraffin-embedded (FFPE) human tissue sections. Flexibly customize panels using your own antibodies with custom conjugation options.
Simultaneously interrogate multiple protein biomarkers. All from a single slide.
100 µm
Alpha smooth muscle actin, HMWK, Ki-67, collagen I CD31, CD34, collagen I beta-catenin, CD3, collagen I vimentin, histone H3 Figure 2. Protein biomarkers in human lung tissue are visualized at subcellular resolution following precise metal-tag capture from a user-defined tissue location and detection at 1 Da spectral resolution using proven CyTOF technology. Markers used with a human lung FFPE tissue sample include alpha-smooth muscle actin (141Pr), high-molecular-weight keratin (144Nd), CD31 (145Nd), beta-catenin (147Sm), vimentin (143Nd), Ki-67 (168Er), CD34 (158Gd), CD3 (170Er), histone H3 (176 Yb), and collagen I (169Tm).
Visualize spatial context of multiplex data in minutes Now that we can measure so many markers The MCD Viewer software that accompanies the Hyperion Imaging System precisely converts the scanned cells and tissues into TIFF images of simultaneously, we for the publication quality. Each image can be spectrally separated to display the first time have the ability to expression of each protein. Data for each region of interest (ROI) can be determine which cell types exported for third-party analysis on software such as histoCAT™. are present in the tissue, and especially in the tumors, Maximize system performance with and also how they are Fluidigm service, support and training spatially arranged. With the Fluidigm service plan, you can boost productivity, control costs and Bernd Bodenmiller, PhD minimize downtime. Service plan options are attractively priced and include Assistant Professor regularly scheduled maintenance visits to ensure that your Hyperion Imaging Institute of Molecular Life Sciences System is kept in peak operating condition. Fluidigm Field Application University of Zurich Specialists provide you with a selection of training programs to help you get started so you can begin asking new questions in your own research with the Hyperion Imaging System.
Learn more at fluidigm.com/hyperionimaging
© 2017 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, CyTOF, Hyperion, Imaging Mass Cytometry, IMC and Maxpar are trademarks or registered trademarks of Fluidigm Corporation in the United States and/or other countries. All other trademarks are the property of their respective owners. 10/2017 For Research Use Only. Not for use in diagnostic procedures.
PN 101-7301 A1 PN 400323 A4 SPECIFICATION SHEET
Hyperion Imaging System
The Hyperion™ Imaging System is a for compensation associated with fluorometric high-parameter imaging system capable of techniques yet far more specific and sensitive analyzing 4 to 37 protein markers at subcellular than tag-free techniques. resolution in fixed tissue sections or cell smears. The metal tags can be defined within 1 Da spatial With the ability to utilize up to 135 channels to resolution in tissue and cell smears, resulting in a detect additional parameters, the Hyperion unique spatial and parametric definition of the Imaging System is ideal to meet researcher cells in situ. The system enables understanding needs today and well into the future. of protein behavior and interactions to drive The Hyperion Imaging System is based on the biological breakthroughs. Helios™ mass cytometry platform. This The Hyperion Imaging System comprises a high-dimensional imaging platform uses Hyperion Tissue Imager, a Helios system and mass-tagged antibodies to markers alongside software for data acquisition and viewing. cell structural features in tissue and cell smears. Maxpar® pathologist-verified antibodies, antibody Mass-tagging involves separation of signals labelling kits and custom antibody conjugation based on the differences in mass, resulting in services are available at fluidigm.com. distinct signals for each marker without the need
Hyperion Imaging System Specifications
Description Specification Description Specification
Channels Cross-cell contamination ≤2% (selected direction) (redeposition) Mass range – Da Crosstalk pixel to pixel ≤15% at 200 pixels/sec Abundance sensitivity .% for Tb Wet tissue thickness ≤7 µm Frequency 200 pixels/sec for full ablation
Detection limit ≥40 copies per µm2 Addressable sample ≥15 mm x 45 mm size on slide Dynamic range orders of magnitude Optical view of sample ≥ 250 µm x 250 µm Ablation spot size ≤ 1µm2 File type TXT, multipage TIFF, Calibration Automated OME-TIFF, MCD
Operating system Windows® Pro -bit Scan area ≥1 mm2/2 hr (@200 Hz)
Data storage >6 TB RAID (mirrored)
Switch time 12 hr (between modes)
Workstation Specifications Monitor Specifications
Description Specification Description Specification
Motherboard HP® Z840 Workstation Monitor LG® 34" Class 21:9 UltraWide® LED Monitor CPU 1 and 2 Intel® Xeon® E5-2643 v4, 3.40 GHz, 2400 6C Screen size 34.1 in (86.6 cm)
RAM 64 GB DDR4-2400 (8 x 8 GB) 2 CPU RegRAM
Graphics card NVIDIA® Quadro® K620 2 GB Graphics (DL-DVI+DP) Data File Size
DVD 9.5 mm Slim SuperMulti File Type Size* DVD-RW 1st ODD MCD 4–5 MB/channel Keyboard HP USB keyboard US TXT 6–8 MB/channel Mouse HP USB optical mouse *1,000 x 1,000 µm region of interest (ROI) Chassis HP Z840 1,125 W (1,450 W/200 V) 90% efficient chassis
RAID controller LSI 9270-8i SAS 6Gb/s ROC RAID Card
Storage drives HP 2 x 256 GB SATA SSD RAID 1
Western Digital® 4 x 4 TB 7200 RPM SATA, RAID 10
2 Hyperion Imaging System: Specification Sheet
Hyperion Tissue Imager Dimensions* Chiller Size
Description Specification Description Specification
Dimensions Width 56 cm (22 in) Dimensions Width cm (15 in)
Height 134 cm (53 in) Height cm (25 in)
Depth 56 cm (22 in) Depth cm (27 in)
Weight 159 kg (350 lb) Weight kg (178 lb)
Helios Dimensions Monitor Size
Description Specification Description Specification
Dimensions Width cm (41 in) Dimensions Width 83.05 cm (32.70 in)
Height cm (52 in) Height 54.86 cm (21.60 in)
Depth 87 cm (35 in) Depth 23.87 cm (9.40 in)
Weight 320 kg (705 lb) Weight 8.40 kg (18.50 lb) with stand
* The Hyperion Tissue Imager must be placed directly in front of the Helios instrument.
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For Research Use Only. Not for use in diagnostic procedures. Information in this publication is subject to change without notice. Patent and license information: fluidigm.com/legalnotices. Fluidigm, the Fluidigm logo, Helios, Hyperion and Maxpar are trademarks or registered trademarks of Fluidigm Corporation in the United States and/or other countries. All other trademarks are the sole property of their respective owners. © 2018 Fluidigm Corporation. All rights reserved. 02/2018