Suplementary Table 1. Protein Profile of the Secretome of MC3T3-E1 Osteoblastic Cells and MLO-Y4 Osteocytic Cells

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Suplementary Table 1. Protein profile of the secretome of MC3T3-E1 osteoblastic cells and MLO-Y4 osteocytic cells. Analysis of the protein secretome by RP-LC- MS/MS reveals absence of mindin (spondin-2) MC3T3-E1 (A) cells or MLO-Y4 (B) cells.

A. MC3T3-E1

# MW calc. Accession Description AAs [kDa] pI P61982;A7Z 14-3-3 protein gamma OS=Mus musculus GN=Ywhag PE=1 SV=2 - 247 28,3 4,89 057 [1433G_MOUSE] P14206 40S ribosomal protein SA OS=Mus musculus GN=Rpsa PE=1 SV=4 - 295 32,8 4,87 [RSSA_MOUSE] P68033;Q3Z Actin, alpha cardiac muscle 1 OS=Mus musculus GN=Actc1 PE=1 SV=1 - 377 42,0 5,39 C07 [ACTC_MOUSE] P60710;P60 Actin, cytoplasmic 1 OS=Mus musculus GN=Actb PE=1 SV=1 - [ACTB_MOUSE] 375 41,7 5,48 712 P50247 Adenosylhomocysteinase OS=Mus musculus GN=Ahcy PE=1 SV=3 - 432 47,7 6,54 [SAHH_MOUSE] P17182 Alpha-enolase OS=Mus musculus GN=Eno1 PE=1 SV=3 - [ENOA_MOUSE] 434 47,1 6,80 B0V2N8 Annexin (Fragment) OS=Mus musculus GN=Anxa2 PE=1 SV=1 - 176 19,6 5,96 [B0V2N8_MOUSE] P10107 Annexin A1 OS=Mus musculus GN=Anxa1 PE=1 SV=2 - [ANXA1_MOUSE] 346 38,7 7,37 P48036 Annexin A5 OS=Mus musculus GN=Anxa5 PE=1 SV=1 - [ANXA5_MOUSE] 319 35,7 4,96 P05202 Aspartate aminotransferase, mitochondrial OS=Mus musculus GN=Got2 PE=1 430 47,4 9,00 SV=1 - [AATM_MOUSE] Q8BFZ3 Beta-actin-like protein 2 OS=Mus musculus GN=Actbl2 PE=1 SV=1 - 376 42,0 5,49 [ACTBL_MOUSE] Q8VCQ8 Caldesmon 1 OS=Mus musculus GN=Cald1 PE=1 SV=1 - [Q8VCQ8_MOUSE] 530 60,4 7,37 Q00493 Carboxypeptidase E OS=Mus musculus GN=Cpe PE=1 SV=2 - [CBPE_MOUSE] 476 53,2 5,19 P10605 Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2 - [CATB_MOUSE] 339 37,3 5,91 P08121 Collagen alpha-1(III) chain OS=Mus musculus GN=Col3a1 PE=1 SV=4 - 146 138,9 6,52 [CO3A1_MOUSE] 4 Q04857 Collagen alpha-1(VI) chain OS=Mus musculus GN=Col6a1 PE=1 SV=1 - 102 108,4 5,36 [CO6A1_MOUSE] 5 Q01149 Collagen alpha-2(I) chain OS=Mus musculus GN=Col1a2 PE=1 SV=2 - 137 129,5 9,19 [CO1A2_MOUSE] 2 Q8CG14 Complement C1s-A subcomponent OS=Mus musculus GN=C1sa PE=2 SV=2 - 688 76,8 5,08 [CS1A_MOUSE] P01027 Complement C3 OS=Mus musculus GN=C3 PE=1 SV=3 - [CO3_MOUSE] 166 186,4 6,73 3 Q61508 Extracellular matrix protein 1 OS=Mus musculus GN=Ecm1 PE=1 SV=2 - 559 62,8 6,80 [ECM1_MOUSE] P26040 Ezrin OS=Mus musculus GN=Ezr PE=1 SV=3 - [EZRI_MOUSE] 586 69,4 6,10 Q61553 Fascin OS=Mus musculus GN=Fscn1 PE=1 SV=4 - [FSCN1_MOUSE] 493 54,5 6,89 Q4KL80 Fibronectin OS=Mus musculus GN=Fn1 PE=1 SV=1 - [Q4KL80_MOUSE] 383 42,3 8,40 P47931 Follistatin OS=Mus musculus GN=Fst PE=2 SV=1 - [FST_MOUSE] 344 37,8 5,53 P47880 Insulin-like growth factor-binding protein 6 OS=Mus musculus GN=Igfbp6 PE=2 238 25,3 8,19 SV=2 - [IBP6_MOUSE] A2A513 Keratin, type I cytoskeletal 10 OS=Mus musculus GN=Krt10 PE=1 SV=1 - 561 57,0 5,07 [A2A513_MOUSE] P06151 L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3 - 332 36,5 7,74 [LDHA_MOUSE] Q02819 Nucleobindin-1 OS=Mus musculus GN=Nucb1 PE=1 SV=2 - [NUCB1_MOUSE] 459 53,4 5,07 G3UZJ4 Peroxiredoxin-5, mitochondrial OS=Mus musculus GN=Prdx5 PE=1 SV=1 - 166 17,2 9,25 [G3UZJ4_MOUSE] D3Z0Y2 Peroxiredoxin-6 OS=Mus musculus GN=Prdx6 PE=1 SV=1 - [D3Z0Y2_MOUSE] 200 22,5 8,88 P97298 Pigment epithelium-derived factor OS=Mus musculus GN=Serpinf1 PE=1 SV=2 - 417 46,2 6,98 [PEDF_MOUSE] P48678 Prelamin-A/C OS=Mus musculus GN=Lmna PE=1 SV=2 - [LMNA_MOUSE] 665 74,2 6,98 Q61398 Procollagen C-endopeptidase enhancer 1 OS=Mus musculus GN=Pcolce PE=1 468 50,1 8,41 SV=2 - [PCOC1_MOUSE] Q9R1P4 Proteasome subunit alpha type-1 OS=Mus musculus GN=Psma1 PE=1 SV=1 - 263 29,5 6,46 [PSA1_MOUSE] P27773 Protein disulfide-isomerase A3 OS=Mus musculus GN=Pdia3 PE=1 SV=2 - 505 56,6 6,21 [PDIA3_MOUSE] P52480 Pyruvate kinase PKM OS=Mus musculus GN=Pkm PE=1 SV=4 - [KPYM_MOUSE] 531 57,8 7,47 Q921I1 Serotransferrin OS=Mus musculus GN=Tf PE=1 SV=1 - [TRFE_MOUSE] 697 76,7 7,18 Q60854 Serpin B6 OS=Mus musculus GN=Serpinb6 PE=1 SV=1 - [SPB6_MOUSE] 378 42,6 5,74 P28862 Stromelysin-1 OS=Mus musculus GN=Mmp3 PE=2 SV=2 - [MMP3_MOUSE] 477 53,8 6,13 Q8BND5 Sulfhydryl oxidase 1 OS=Mus musculus GN=Qsox1 PE=1 SV=1 - 748 82,7 7,17 [QSOX1_MOUSE] P40142 Transketolase OS=Mus musculus GN=Tkt PE=1 SV=1 - [TKT_MOUSE] 623 67,6 7,50 H7BXC3 Triosephosphate isomerase OS=Mus musculus GN=Tpi1 PE=1 SV=1 - 167 18,0 5,58 [H7BXC3_MOUSE]

B. MLO-Y4

# MW calc. Accession Description AAs [kDa] pI P62259;P6 14-3-3 protein epsilon OS=Mus musculus GN=Ywhae PE=1 SV=1 - [1433E_MOUSE] 255 29,2 4,74 2261 Q9DCD0 6-phosphogluconate dehydrogenase, decarboxylating OS=Mus musculus GN=Pgd 483 53,2 7,23 PE=1 SV=3 - [6PGD_MOUSE] P20029 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3 - 655 72,4 5,16 [GRP78_MOUSE] P68033;Q3 Actin, alpha cardiac muscle 1 OS=Mus musculus GN=Actc1 PE=1 SV=1 - 377 42,0 5,39 ZC07 [ACTC_MOUSE] P60710;P6 Actin, cytoplasmic 1 OS=Mus musculus GN=Actb PE=1 SV=1 - [ACTB_MOUSE] 375 41,7 5,48 0712 P50247 Adenosylhomocysteinase OS=Mus musculus GN=Ahcy PE=1 SV=3 - 432 47,7 6,54 [SAHH_MOUSE] P45376 Aldose reductase OS=Mus musculus GN=Akr1b1 PE=1 SV=3 - [ALDR_MOUSE] 316 35,7 7,18 P17182 Alpha-enolase OS=Mus musculus GN=Eno1 PE=1 SV=3 - [ENOA_MOUSE] 434 47,1 6,80 B0V2N7 Annexin (Fragment) OS=Mus musculus GN=Anxa2 PE=1 SV=1 - [B0V2N7_MOUSE] 196 21,8 5,35 P10107 Annexin A1 OS=Mus musculus GN=Anxa1 PE=1 SV=2 - [ANXA1_MOUSE] 346 38,7 7,37 P48036 Annexin A5 OS=Mus musculus GN=Anxa5 PE=1 SV=1 - [ANXA5_MOUSE] 319 35,7 4,96 F7ALS6 Aspartate aminotransferase, cytoplasmic (Fragment) OS=Mus musculus GN=Got1 182 20,4 5,85 PE=1 SV=1 - [F7ALS6_MOUSE] P05202 Aspartate aminotransferase, mitochondrial OS=Mus musculus GN=Got2 PE=1 SV=1 430 47,4 9,00 - [AATM_MOUSE] P01887 Beta-2-microglobulin OS=Mus musculus GN=B2m PE=1 SV=2 - [B2MG_MOUSE] 119 13,8 8,44 P10605 Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2 - [CATB_MOUSE] 339 37,3 5,91 P06797 Cathepsin L1 OS=Mus musculus GN=Ctsl PE=1 SV=2 - [CATL1_MOUSE] 334 37,5 6,83 P18760 Cofilin-1 OS=Mus musculus GN=Cfl1 PE=1 SV=3 - [COF1_MOUSE] 166 18,5 8,09 P11087 Collagen alpha-1(I) chain OS=Mus musculus GN=Col1a1 PE=1 SV=4 - 145 137,9 5,85 [CO1A1_MOUSE] 3 Q04857 Collagen alpha-1(VI) chain OS=Mus musculus GN=Col6a1 PE=1 SV=1 - 102 108,4 5,36 [CO6A1_MOUSE] 5 Q8CG16 Complement C1r-A subcomponent OS=Mus musculus GN=C1ra PE=1 SV=1 - 707 80,0 5,66 [C1RA_MOUSE] P01027 Complement C3 OS=Mus musculus GN=C3 PE=1 SV=3 - [CO3_MOUSE] 166 186,4 6,73 3 O08553 Dihydropyrimidinase-related protein 2 OS=Mus musculus GN=Dpysl2 PE=1 SV=2 - 572 62,2 6,38 [DPYL2_MOUSE] P58252 Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2 - [EF2_MOUSE] 858 95,3 6,83 Q61508 Extracellular matrix protein 1 OS=Mus musculus GN=Ecm1 PE=1 SV=2 - 559 62,8 6,80 [ECM1_MOUSE] B7ZNJ1 Fibronectin OS=Mus musculus GN=Fn1 PE=1 SV=1 - [B7ZNJ1_MOUSE] 217 239,6 6,10 6 P05064 Fructose-bisphosphate aldolase A OS=Mus musculus GN=Aldoa PE=1 SV=2 - 364 39,3 8,09 [ALDOA_MOUSE] Q07797 Galectin-3-binding protein OS=Mus musculus GN=Lgals3bp PE=1 SV=1 - 577 64,4 5,14 [LG3BP_MOUSE] P13020 Gelsolin OS=Mus musculus GN=Gsn PE=1 SV=3 - [GELS_MOUSE] 780 85,9 6,18 P06745 Glucose-6-phosphate isomerase OS=Mus musculus GN=Gpi PE=1 SV=4 - 558 62,7 8,13 [G6PI_MOUSE] D3YX76 Glutathione S-transferase Mu 2 OS=Mus musculus GN=Gstm2 PE=1 SV=1 - 184 21,7 7,84 [D3YX76_MOUSE] Q9CZD3 Glycine--tRNA ligase OS=Mus musculus GN=Gars PE=1 SV=1 - [SYG_MOUSE] 729 81,8 6,65 P62827;Q3 GTP-binding nuclear protein Ran OS=Mus musculus GN=Ran PE=1 SV=3 - 216 24,4 7,49 T054 [RAN_MOUSE] P49312;P0 Heterogeneous nuclear ribonucleoprotein A1 OS=Mus musculus GN=Hnrnpa1 PE=1 320 34,2 9,23 9867 SV=2 - [ROA1_MOUSE] P30681 High mobility group protein B2 OS=Mus musculus GN=Hmgb2 PE=1 SV=3 - 210 24,1 7,31 [HMGB2_MOUSE] P70168 Importin subunit beta-1 OS=Mus musculus GN=Kpnb1 PE=1 SV=2 - 876 97,1 4,78 [IMB1_MOUSE] Q04998 Inhibin beta A chain OS=Mus musculus GN=Inhba PE=1 SV=1 - [INHBA_MOUSE] 424 47,4 8,03 P47879 Insulin-like growth factor-binding protein 4 OS=Mus musculus GN=Igfbp4 PE=1 254 27,8 7,17 SV=2 - [IBP4_MOUSE] A2A513 Keratin, type I cytoskeletal 10 OS=Mus musculus GN=Krt10 PE=1 SV=1 - 561 57,0 5,07 [A2A513_MOUSE] Q61781 Keratin, type I cytoskeletal 14 OS=Mus musculus GN=Krt14 PE=1 SV=2 - 484 52,8 5,17 [K1C14_MOUSE] D3Z736 L-lactate dehydrogenase OS=Mus musculus GN=Ldha PE=1 SV=1 - 237 26,0 8,07 [D3Z736_MOUSE] O08746 Matrilin-2 OS=Mus musculus GN=Matn2 PE=2 SV=2 - [MATN2_MOUSE] 956 106,7 6,58 P26041 Moesin OS=Mus musculus GN=Msn PE=1 SV=3 - [MOES_MOUSE] 577 67,7 6,60 P11672 Neutrophil gelatinase-associated lipocalin OS=Mus musculus GN=Lcn2 PE=1 SV=1 - 200 22,9 8,81 [NGAL_MOUSE] Q02819 Nucleobindin-1 OS=Mus musculus GN=Nucb1 PE=1 SV=2 - [NUCB1_MOUSE] 459 53,4 5,07 P09405 Nucleolin OS=Mus musculus GN=Ncl PE=1 SV=2 - [NUCL_MOUSE] 707 76,7 4,75 P48759 Pentraxin-related protein PTX3 OS=Mus musculus GN=Ptx3 PE=1 SV=2 - 381 41,8 5,38 [PTX3_MOUSE] G3UZJ4 Peroxiredoxin-5, mitochondrial OS=Mus musculus GN=Prdx5 PE=1 SV=1 - 166 17,2 9,25 [G3UZJ4_MOUSE] D3Z0Y2 Peroxiredoxin-6 OS=Mus musculus GN=Prdx6 PE=1 SV=1 - [D3Z0Y2_MOUSE] 200 22,5 8,88 Q99K85 Phosphoserine aminotransferase OS=Mus musculus GN=Psat1 PE=1 SV=1 - 370 40,4 8,03 [SERC_MOUSE] P97298 Pigment epithelium-derived factor OS=Mus musculus GN=Serpinf1 PE=1 SV=2 - 417 46,2 6,98 [PEDF_MOUSE] P48678 Prelamin-A/C OS=Mus musculus GN=Lmna PE=1 SV=2 - [LMNA_MOUSE] 665 74,2 6,98 A0A0G2JE Procollagen C-endopeptidase enhancer 1 (Fragment) OS=Mus musculus GN=Pcolce 246 26,4 9,38 29 PE=1 SV=1 - [A0A0G2JE29_MOUSE] Q5SX49 Profilin OS=Mus musculus GN=Pfn1 PE=1 SV=1 - [Q5SX49_MOUSE] 112 11,8 4,88 E9PZ00 Prosaposin OS=Mus musculus GN=Psap PE=1 SV=1 - [E9PZ00_MOUSE] 551 60,6 5,24 Q91YR9 Prostaglandin reductase 1 OS=Mus musculus GN=Ptgr1 PE=1 SV=2 - 329 35,5 7,96 [PTGR1_MOUSE] P27773 Protein disulfide-isomerase A3 OS=Mus musculus GN=Pdia3 PE=1 SV=2 - 505 56,6 6,21 [PDIA3_MOUSE] P23492 Purine nucleoside phosphorylase OS=Mus musculus GN=Pnp PE=1 SV=2 - 289 32,3 6,16 [PNPH_MOUSE] P52480 Pyruvate kinase PKM OS=Mus musculus GN=Pkm PE=1 SV=4 - [KPYM_MOUSE] 531 57,8 7,47 Q921I1 Serotransferrin OS=Mus musculus GN=Tf PE=1 SV=1 - [TRFE_MOUSE] 697 76,7 7,18 E9PYY0 Serpin B6 (Fragment) OS=Mus musculus GN=Serpinb6a PE=1 SV=1 - 120 12,8 5,41 [E9PYY0_MOUSE] Q5NCU4 SPARC OS=Mus musculus GN=Sparc PE=1 SV=1 - [Q5NCU4_MOUSE] 301 34,3 4,86 P28862 Stromelysin-1 OS=Mus musculus GN=Mmp3 PE=2 SV=2 - [MMP3_MOUSE] 477 53,8 6,13 Q8BND5 Sulfhydryl oxidase 1 OS=Mus musculus GN=Qsox1 PE=1 SV=1 - [QSOX1_MOUSE] 748 82,7 7,17 P82198 Transforming growth factor-beta-induced protein ig-h3 OS=Mus musculus GN=Tgfbi 683 74,5 7,06 PE=1 SV=1 - [BGH3_MOUSE] P40142 Transketolase OS=Mus musculus GN=Tkt PE=1 SV=1 - [TKT_MOUSE] 623 67,6 7,50 P17751 Triosephosphate isomerase OS=Mus musculus GN=Tpi1 PE=1 SV=4 - 299 32,2 5,74 [TPIS_MOUSE] P99024;Q2 Tubulin beta-5 chain OS=Mus musculus GN=Tubb5 PE=1 SV=1 - [TBB5_MOUSE] 444 49,6 4,89 KJD0 A0A0G2JFP Vascular cell adhesion protein 1 (Fragment) OS=Mus musculus GN=Vcam1 PE=1 237 26,4 5,31 9 SV=1 - [A0A0G2JFP9_MOUSE] A0A0A6YW Vimentin OS=Mus musculus GN=Vim PE=1 SV=1 - [A0A0A6YWC8_MOUSE] 427 49,2 5,12 C8