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S-Nitrosylation in Organs of Mice Exposed to Low Or High Doses of Γ-Rays

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S-Nitrosylation in Organs of Mice Exposed to Low Or High Doses of Γ-Rays Supplementary Materials Table 1. Identification of streptavidin enriched biotinylated proteins from brain of 17 week-old C57BL/6J mice exposed to 0, 0.1 or 4 Gy of 137Cs γ-rays 13 days earlier. Equal mass aliquots of protein lysates from 5 different mice in each group were combined, modified by the biotin switch assay, and submitted for mass spectrometry analyses. Molecular Quantitative Value Protein Descriptions Protein Name (Swissprot) Weight 0 cGy 0.1 Gy 4 Gy 14-3-3 protein gamma OS = Mus musculus 1433G_MOUSE 28 kDa 4.69 7.01 4.64 GN = Ywhag PE = 1 SV = 2 14-3-3 protein zeta/delta OS = Mus musculus 1433Z_MOUSE 28 kDa 14.06 12.85 10.21 GN = Ywhaz PE = 1 SV = 1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS = Mus musculus PLCB1_MOUSE 138 kDa 4.69 4.67 1.86 GN = Plcb1 PE = 1 SV = 2 2',3'-cyclic-nucleotide 3'-phosphodiesterase CN37_MOUSE 47 kDa 6.56 5.84 11.14 OS = Mus musculus GN = Cnp PE = 1 SV = 3 2-oxoglutarate dehydrogenase, mitochondrial ODO1_MOUSE 116 kDa 3.75 4.67 4.64 OS = Mus musculus GN = Ogdh PE = 1 SV = 3 3-hydroxyisobutyrate dehydrogenase, mitochondrial 3HIDH_MOUSE 35 kDa 0.94 4.67 0.93 OS = Mus musculus GN = Hibadh PE = 2 SV = 1 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial HIBCH_MOUSE 43 kDa 0.00 1.17 3.71 OS = Mus musculus GN = Hibch PE = 1 SV = 1 3-mercaptopyruvate sulfurtransferase OS = Mus THTM_MOUSE 33 kDa 5.62 4.67 3.71 musculus GN = Mpst PE = 1 SV = 3 40S ribosomal protein S10 OS = Mus musculus RS10_MOUSE 19 kDa 6.56 5.84 3.71 GN = Rps10 PE = 1 SV = 1 40S ribosomal protein S2 OS = Mus musculus RS2_MOUSE 31 kDa 2.81 2.34 3.71 GN = Rps2 PE = 1 SV = 3 40S ribosomal protein S3a OS = Mus musculus RS3A_MOUSE 30 kDa 1.87 4.67 0.00 GN = Rps3a PE = 1 SV = 3 4-aminobutyrate aminotransferase, mitochondrial GABT_MOUSE 56 kDa 13.12 16.35 12.07 OS = Mus musculus GN = Abat PE = 1 SV = 1 4F2 cell-surface antigen heavy chain OS = Mus 4F2_MOUSE 58 kDa 3.75 3.50 2.79 musculus GN = Slc3a2 PE = 1 SV = 1 5'-nucleotidase domain-containing protein 3 NT5D3_MOUSE 63 kDa 3.75 3.50 3.71 OS = Mus musculus GN = Nt5dc3 PE = 2 SV = 1 60 kDa heat shock protein, mitochondrial CH60_MOUSE 61 kDa 2.81 1.17 0.00 OS = Mus musculus GN = Hspd1 PE = 1 SV = 1 60S ribosomal protein L5 OS = Mus musculus RL5_MOUSE 34 kDa 3.75 3.50 4.64 GN = Rpl5 PE = 1 SV = 3 6-phosphofructokinase, liver type OS = Mus K6PL_MOUSE 85 kDa 3.75 0.00 5.57 musculus GN = Pfkl PE = 1 SV = 4 6-phosphofructokinase, muscle type OS = Mus K6PF_MOUSE 85 kDa 6.56 2.34 7.43 musculus GN = Pfkm PE = 1 SV = 3 Aconitate hydratase, mitochondrial OS = Mus ACON_MOUSE 85 kDa 8.44 5.84 13.00 musculus GN = Aco2 PE = 1 SV = 1 S2 Table S1. Cont. Molecular Quantitative Value Protein Descriptions Protein Name (Swissprot) Weight 0 cGy 0.1 Gy 4 Gy Actin, cytoplasmic 1 OS = Mus musculus ACTB_MOUSE (+1) 42 kDa 68.43 73.59 56.63 GN = Actb PE = 1 SV = 1 Actin-related protein 2 OS = Mus musculus ARP2_MOUSE 45 kDa 17.81 17.52 18.57 GN = Actr2 PE = 1 SV = 1 Actin-related protein 2/3 complex subunit 2 ARPC2_MOUSE 34 kDa 1.87 9.35 1.86 OS = Mus musculus GN = Arpc2 PE = 1 SV = 3 Actin-related protein 2/3 complex subunit 4 ARPC4_MOUSE 20 kDa 6.56 15.19 9.28 OS = Mus musculus GN = Arpc4 PE = 1 SV = 3 Actin-related protein 3 OS = Mus musculus ARP3_MOUSE 47 kDa 12.19 10.51 16.71 GN = Actr3 PE = 1 SV = 3 Actin-related protein 3B OS = Mus musculus ARP3B_MOUSE 48 kDa 10.31 10.51 14.85 GN = Actr3b PE = 2 SV = 1 Active breakpoint cluster region-related protein ABR_MOUSE 98 kDa 1.87 0.00 2.79 OS = Mus musculus GN = Abr PE = 2 SV = 1 Adapter molecule crk OS = Mus musculus CRK_MOUSE 34 kDa 2.81 4.67 3.71 GN = Crk PE = 1 SV = 1 Adenylate kinase isoenzyme 1 OS = Mus musculus KAD1_MOUSE 22 kDa 3.75 1.17 1.86 GN = Ak1 PE = 1 SV = 1 ADP/ATP translocase 1 OS = Mus musculus ADT1_MOUSE 33 kDa 30.00 47.89 37.14 GN = Slc25a4 PE = 1 SV = 4 ADP/ATP translocase 2 OS = Mus musculus ADT2_MOUSE 33 kDa 27.18 31.54 24.14 GN = Slc25a5 PE = 1 SV = 3 ADP-ribosylation factor 5 OS = Mus musculus ARF5_MOUSE 21 kDa 108.74 115.64 150.40 GN = Arf5 PE = 2 SV = 2 Alanine--tRNA ligase, cytoplasmic OS = Mus SYAC_MOUSE 107 kDa 0.94 2.34 0.93 musculus GN = Aars PE = 1 SV = 1 Aldose reductase OS = Mus musculus ALDR_MOUSE 36 kDa 4.69 0.00 4.64 GN = Akr1b1 PE = 1 SV = 3 Alpha-actinin-4 OS = Mus musculus GN = Actn4 ACTN4_MOUSE 105 kDa 7.50 7.01 3.71 PE = 1 SV = 1 Alpha-adducin OS = Mus musculus GN = Add1 ADDA_MOUSE 81 kDa 3.75 5.84 2.79 PE = 1 SV = 2 Alpha-enolase OS = Mus musculus GN = Eno1 ENOA_MOUSE 47 kDa 51.56 35.04 29.71 PE = 1 SV = 3 Alpha-internexin OS = Mus musculus GN = Ina AINX_MOUSE 56 kDa 3.75 0.00 1.86 PE = 1 SV = 2 Alpha-synuclein OS = Mus musculus GN = Snca SYUA_MOUSE 14 kDa 3.75 0.00 1.86 PE = 1 SV = 2 Ankyrin-2 OS = Mus musculus GN = Ank2 ANK2_MOUSE 426 kDa 1.87 3.50 1.86 PE = 1 SV = 2 Annexin A2 OS = Mus musculus GN = Anxa2 ANXA2_MOUSE 39 kDa 2.81 0.00 0.00 PE = 1 SV = 2 S3 Table S1. Cont. Molecular Quantitative Value Protein Descriptions Protein Name (Swissprot) Weight 0 cGy 0.1 Gy 4 Gy AP-1 complex subunit mu-1 OS = Mus musculus AP1M1_MOUSE 49 kDa 5.62 0.00 2.79 GN = Ap1m1 PE = 1 SV = 3 AP-2 complex subunit alpha-1 OS = Mus musculus AP2A1_MOUSE 108 kDa 8.44 5.84 13.93 GN = Ap2a1 PE = 1 SV = 1 AP-2 complex subunit alpha-2 OS = Mus musculus AP2A2_MOUSE 104 kDa 8.44 11.68 11.14 GN = Ap2a2 PE = 1 SV = 2 AP-2 complex subunit beta OS = Mus musculus AP2B1_MOUSE 105 kDa 7.50 7.01 13.00 GN = Ap2b1 PE = 1 SV = 1 AP-2 complex subunit mu OS = Mus musculus AP2M1_MOUSE 50 kDa 10.31 7.01 8.36 GN = Ap2m1 PE = 1 SV = 1 AP-2 complex subunit sigma OS = Mus musculus AP2S1_MOUSE 17 kDa 6.56 4.67 5.57 GN = Ap2s1 PE = 1 SV = 1 Aspartate aminotransferase, cytoplasmic AATC_MOUSE 46 kDa 32.81 46.73 27.85 OS = Mus musculus GN = Got1 PE = 1 SV = 3 Aspartate aminotransferase, mitochondrial AATM_MOUSE 47 kDa 12.19 12.85 11.14 OS = Mus musculus GN = Got2 PE = 1 SV = 1 Atlastin-1 OS = Mus musculus GN = Atl1 PE = 1 ATLA1_MOUSE 63 kDa 7.50 4.67 5.57 SV = 1 ATP synthase subunit alpha, mitochondrial ATPA_MOUSE 60 kDa 34.68 49.06 38.99 OS = Mus musculus GN = Atp5a1 PE = 1 SV = 1 ATP synthase subunit b, mitochondrial OS = Mus AT5F1_MOUSE 29 kDa 25.31 33.88 25.07 musculus GN = Atp5f1 PE = 1 SV = 1 ATP synthase subunit beta, mitochondrial OS = Mus ATPB_MOUSE 56 kDa 31.87 18.69 25.07 musculus GN = Atp5b PE = 1 SV = 2 ATP synthase subunit d, mitochondrial OS = Mus ATP5H_MOUSE 19 kDa 10.31 0.00 12.07 musculus GN = Atp5h PE = 1 SV = 3 ATP synthase subunit gamma, mitochondrial ATPG_MOUSE 33 kDa 15.00 7.01 15.78 OS = Mus musculus GN = Atp5c1 PE = 1 SV = 1 Band 4.1-like protein 3 OS = Mus musculus E41L3_MOUSE 103 kDa 5.62 2.34 3.71 GN = Epb41l3 PE = 1 SV = 1 Beta-actin-like protein 2 OS = Mus musculus ACTBL_MOUSE 42 kDa 26.25 16.35 23.21 GN = Actbl2 PE = 1 SV = 1 Beta-adducin OS = Mus musculus GN = Add2 ADDB_MOUSE 81 kDa 1.87 0.00 0.93 PE = 1 SV = 4 Beta-adrenergic receptor kinase 1 OS = Mus ARBK1_MOUSE 80 kDa 1.87 2.34 2.79 musculus GN = Adrbk1 PE = 2 SV = 2 Beta-centractin OS = Mus musculus GN = Actr1b ACTY_MOUSE (+1) 42 kDa 5.62 4.67 5.57 PE = 1 SV = 1 Beta-hexosaminidase subunit beta OS = Mus HEXB_MOUSE 61 kDa 1.87 3.50 2.79 musculus GN = Hexb PE = 2 SV = 2 Beta-soluble NSF attachment protein OS = Mus SNAB_MOUSE 34 kDa 5.62 1.17 5.57 musculus GN = Napb PE = 1 SV = 2 S4 Table S1. Cont. Molecular Quantitative Value Protein Descriptions Protein Name (Swissprot) Weight 0 cGy 0.1 Gy 4 Gy Beta-synuclein OS = Mus musculus GN = Sncb SYUB_MOUSE 14 kDa 3.75 5.84 3.71 PE = 1 SV = 1 Brain protein 44 OS = Mus musculus GN = Brp44 BR44_MOUSE ? 6.56 4.67 4.64 PE = 1 SV = 1 Brevican core protein OS = Mus musculus PGCB_MOUSE 96 kDa 1.87 2.34 3.71 GN = Bcan PE = 1 SV = 2 BTB/POZ domain-containing protein 17 BTBDH_MOUSE 53 kDa 2.81 2.34 2.79 OS = Mus musculus GN = Btbd17 PE = 2 SV = 1 BTB/POZ domain-containing protein KCTD16 KCD16_MOUSE 49 kDa 3.75 7.01 3.71 OS = Mus musculus GN = Kctd16 PE = 1 SV = 2 Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A OS = Mus PDE1A_MOUSE (+1) 65 kDa 3.75 2.34 0.00 musculus GN = Pde1a PE = 2 SV = 2 Calcium/calmodulin-dependent protein kinase type II subunit alpha OS = Mus musculus KCC2A_MOUSE 54 kDa 41.25 78.26 58.49 GN = Camk2a PE = 1 SV = 2 Calcium/calmodulin-dependent protein kinase type II subunit beta OS = Mus musculus KCC2B_MOUSE 60 kDa 9.37 7.01 6.50 GN = Camk2b PE = 1 SV = 2 Calcium/calmodulin-dependent protein kinase type II subunit delta OS = Mus musculus KCC2D_MOUSE 56 kDa 6.56 4.67 1.86 GN = Camk2d PE = 1 SV = 1 Calcium-activated potassium channel subunit alpha-1 OS = Mus musculus GN = Kcnma1 KCMA1_MOUSE 134 kDa 3.75 8.18 6.50 PE = 1 SV = 2 Calcium-binding mitochondrial carrier protein Aralar1 OS = Mus musculus GN = Slc25a12 CMC1_MOUSE 75 kDa 9.37 9.35 11.14 PE = 1 SV = 1 Calcium-dependent secretion activator 1 CAPS1_MOUSE 153 kDa 11.25 25.70 13.00 OS = Mus musculus GN = Cadps PE = 1 SV = 3 Calmodulin OS = Mus musculus GN = Calm1 CALM_MOUSE 17 kDa 1.87 0.00 1.86 PE = 1 SV = 2 Calreticulin OS = Mus musculus GN = Calr CALR_MOUSE 48 kDa 10.31 1.17 6.50 PE = 1 SV = 1 cAMP-dependent protein kinase catalytic subunit KAPCA_MOUSE (+1) 41 kDa 3.75 8.18 0.00 alpha OS = Mus musculus GN = Prkaca PE = 1 SV = 3 Carbonic anhydrase 2 OS = Mus musculus CAH2_MOUSE 29 kDa 7.50 7.01 5.57 GN = Ca2 PE = 1 SV = 4 Casein kinase II subunit alpha' OS = Mus musculus CSK22_MOUSE 41 kDa 0.00 3.50 2.79 GN = Csnk2a2 PE = 2 SV = 1 Cathepsin B OS = Mus musculus GN = Ctsb CATB_MOUSE 37 kDa 6.56 3.50 5.57 PE = 1 SV = 2 S5 Table S1.
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