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Home , Algestone acetophenide, Hormonal contraception

J. Exp. Zool. Vol. 22, No. 2, pp. 1029-1030, 2019 www.connectjournals.com/jez ISSN 0972-0030 MODULATION OF TRIGLYCERIDES IN FEMALE RATTUS RATTUS WITH CONTRACEPTIVE ACETOPHENIDE (DHPA)

Ashok Kumar Department of Zoology, M.L.K. College, Balrampur - 271 201, India. e-mail : [email protected] (Received 18 March 2019, Accepted 30 May 2019)

ABSTRACT : Young female Black rat (Rattus rattus) were administered monthly long acting steroid contraceptive to induce hypertriglyceridemia. It was observed that by 3 weeks of the second injection of containing mixed type of contraceptive, female rats developed consistent and frank hyperglyceridemia. TG in the treated rats was 195.8 ± 7.44 mg /100 ml as compared to 91.5 ± 6.27 mg/100ml in plasma of the control group.

Key words : Rattus rattus, , mood swings, body weight.

INTRODUCTION 100mg dihydroxyprogesterone acetophenide and 5mg Algestone acetophenide, more commonly known as enanthate; animals in DP group were injected dihydroxyprogesterone acetophenide (DHPA) is 0.1ml/ animals/month, a preparation containing 150mg/ a progestin , which is used in combination with ml medroxyprogestrone acetate (Liang et al, 1983). an estrogen as a form of long-lasting injectable birth Animals in the control group were treated with the same control. DHPA is not active by oral administration, so amount physiological saline. Three weeks after the second injected intramuscularly once a month. It has side effects injection, animals were fasted overnight. Sampling from like , , hair loss, decreased sex drive the lateral saphenous vein is a relatively quick method of (Pastor et al, 2013), mood swings (Toffoletto et al, 2014) obtaining blood samples 1.0 ml from each rat. Blood flow and changes in body weight. Hypertriglyceridemia is a is stopped by gentle finger pressure over the punctured major effect of ingesting estrogen containing steroid site. Animals were returned to their cage after the blood contraceptive. The present report deals with our attempts flow has stopped. The blood was collected from lateral to induce frank hypertriglyceridemia in female rats by saphenous vein with the help of microsyringe in to the treatment with long acting steroid contraceptives. sterilized vial containing EDTA (Ethylene diamine tetra acetic acid) at concentration of 1 mg/5 ml MATERIALS AND METHODS of blood. The extracts were used for estimation of In the present investigation, healthy mature female triglycerides, cholesterol and phospholipids. Black rat (Rattus rattus), (110±20gm wt) were used. RESULTS AND DISCUSSION They were maintained in well ventilated and sterilized animal cage (35 cm L × 25 cm W × 18 cm H) with a As shown in Table 1, treatment of female rats with constant 12 hours light and dark schedules in laboratory. mixed type of steroid contraceptive resulted in significant They were fed regularly ad libitum with the food (a increase in the concentration of plasma triglycerides, mixture of cereals, vegetables and cheese) 5 gm three plasma cholesterol and phospholipids. Levels of plasma times per day. (They eat about 15 grams (0.53 oz) per lipids of rats treated with showed day and drink about 15 milliliters (0.53 imp fl oz; 0.51 US no effect on TG, but both cholesterol and phospholipids fl oz) per day (Bennet et al, 2011). Their diet is high in increased significantly. water content (Marsh et al, 1994). They were grouped The dose of steroid contraceptive used in this report in to three groups viz control, mixed and DP and is not excessive since five times the human dose (on acclimatized at room temperature (26±20C). Animals weight to weight basis) has been reported to be required were categorized into three groups: rats in mixed group to inhibit in rodents. Induction of were administered 0.1ml/animal /month, a long acting hypertriglyceridema 3 weeks after the second injection steroid contraceptive preparation containing in each ml of the steroid contraceptive seems to be consistent with 1030 Ashok Kumar

Graph 1 : Modulation of Triglycerides, Cholesterol and Phospholipids in female Rattus rattus with Steroid Contraceptive dihydroxyprogesterone acetophenide (DHPA).

Table 1 : Lipid Profile of female rat (Rattus rattus) treated with REFERENCES steroid contraceptives. Bennet Stuart M (2011) The Black Rat (Rattus rattus). The Pied Plasma Piper Group . Retrieved 22 April 2011. Triglyceride Cholesterol Phospholipids Bostofte E, Hemmingsen L, Moller K J and Weber J (1978) Acta (TG) (mg/100ml) (PLP) Endocrinol. 87, 855. Control 91.5± 6.27 83.0 ± 3.93 88.0 ± 5.4 Liang A P, Levenson A G and Layde P M (1983) Risk of breast, Mixed 195.8 ± 7.44 101.0 ± 5.86 169.0 ± 9.60 uterine corpus, and in women receiving medroxyprogesterone injections. J. Amer. Medical Assoc. 249, P<0.001 P<0.05 P<0.001 2909 –2912. DP 82.1 ± 6.09 100.5 ± 2.17 126.0 ± 6.23 Marsh Rex E (1994) Roof Rats. Internet Center for Wildlife Damage NS P<0.001 P < 0.01 Management. Prevention and Control of Wildlife Damage. *Animals in control group were treated with normal saline; in the Retrieved 22 April, 2011. mixed group with estrogen containing mixed contraceptive and in Pastor Z, Holla K and Chmel R (2013) The influence of combined oral DP group with medroxy- acetate. contraceptives on female sexual desire: A systematic review. The European J. Contracep. Repro. Health Care 18(1), 27–43. the earlier reports that it takes 4-6 weeks for lipids to Toffoletto S, Lanzenberger R, Gingnell M, Sundström-Poromaa I and reach a steady state after treatment with steroid Comasco E (2014) Emotional and cognitive functional imaging contraceptives (Bostofte et al, 1978). of estrogen and progesterone effects in the female human brain: Data presented in this report demonstrate that mixed A systematic review. Psychoneuroendocrinol. 50, 28–52. contraceptive induce a frank hypertriglyceridemia in Wang Q, Würtz P, Auro K, Morin-Papunen L, Kangas A J, Soininen P and Aalto K (2016) Effects of on female rats (Wang et al, 2016). systemic metabolism: cross-sectional and longitudinal evidence. Int. J. Epidemiol. 45(5), 1445–1457.