Litopenaeus Vannamei (Mugil Cephalus

Investigation on feasibility polyculture of Litopenaeus vannamei with Mugil cephalus in earthen shrimp ponds

Item Type monograph

Authors Hosseini Aghouzini, Seyed Hossein; Hafezieh, M.; Ezhdehakoshpor, A.; Mousavi Gelsefid, S.A.; Azhang, B.; Jahantigh, H.; Matinfar, A.; Azhir, M.

Publisher Iranian Fisheries Science Research Institute

Download date 09/10/2021 14:59:38

Link to Item http://hdl.handle.net/1834/39717

(Litopenaeus vannamei (Mugil cephalus)

(Litopenaeus vannamei Mugil cephalus

Mugil cephalus (Litopenaeus vannamei

Litopenaeus vannamei

Mugil cephalus

(NO2

(NO3

(NH3

(PO4

(Biological Oxygen Demand)BOD5 ......

...... a ......

(Litopenaeus vannamei

(T2) (T1) (Mugil cephalus)

(T3)

BOD5

ANOVA

(P<0/05)

T3 (P>0/05)

(P<0/05)

(P>0/05) T1 T2

T3 FCR (P<0/05) T3

T1 FCR

(P>0/05) T3 T2 BOD5 PH

(P<0/05) T1

Bacillariophyceae

Dinophyceae Cyanophyceae

T3 T2

V.Fluvialis V.Alginoliticus

Mugil cephalus Litopenaeus vannamei

Milstein, 1992

Bardach et al., 1972)

(Jhingran, 1982 & Lin, 1982)

.(Milstein,1992)

(Wang, 1990 ; Hopkins et al., 1993; Nelson et al., 2001 ; Tanyaros, 2001; Jones et al., 2001,2002 ; Marinho-

Soriano et al., 2002)

Penaeus japonicas Ulva

(Joseph et al., 2006)

(Eldani & Primavera, 1981 ; Pudadara & Lim, 1982;

Marichamy & Rajapackram, 1982 ; Apud, 1985 ; Kuntiyo & Baliao, 1987; James, 1996 ; Tookwinas, 2003)

. (Milstein,1992)

(Chein,1992)

(Primavera,1994)

Mugil cephalus

(James,1991)

(Smith & Heemstra,1986)

(Mugil cephalus) (Lpitopenaeus vannamei

Litopenaeus vannamei

White leg shrimp Litopenaeus vannamei

(FAO Fishery Statistics, 2006)

(Litopenaeus vannamei) (Wyban & Sweeney.,1991) Arthropoda Crustacean Malacostraca Eumolacostraca eucarida Decapoda Dendrobranchiata Penaeidea Penaeiodea Penaeidea Penaeus litopenaeus Vannanei

(SPF) (SPR)

(Wyban &

Sweeney.,1991)

Brigges & Smith., 2004

Litopenaeus vannamei Penaeus monodon Penaeus merguensis Penaeus japonicas Penaeus chinensis Penaeus indicus Penaeus stylirostris

Mugil cephalus

Thomson, 1963

Fry

Thomson, 1963 ppt

M. cephalus Greenwood

Osteichthyes

Ray finned Acthnopterygii

Mugilidae Mugiloidei

Mugilidae Mugilidae

Mugilinae Agonostominae

M. cephalus Mugilinae

M. cephalus

Mugil cephalus

Microphagous omnivorous

Zismann, 1981

Mugil Pyloric caeca

Zismann, 1981

(Thomson,1963) Hora & Pillay,1962

Mugil cephalus (FAO Fishery Statistics, 2006)

Bait fish

(FAO Fishery Statistics, 2006)

Detritus

Bioremovable (Abdel Tawwab et al., 2005)

(Lupatsch et al.,

2003)

ppm

T3 T2 T2 T1 T3 T2 T1 T1

(gr)

(kg)

(TOM)

(Holme & Mcintyr, 1984)

A B

A C =C

1.Total organic mater

(MOOPAM,1999)

(Carmelu,1977 & Sourina.A, 1978; Standard method, 1998)

(ml)

Standard

Newell, 1977 & Boltovsky, 1999 method, 1998

(ml) (ml)

(ROPME,1987)

nm nm nm

a nm M1

a E E E

WTW-330i PH

Clesceri et al., 1989

Oxygouard

WTW-320

(ppt)

(NO3)

Grassoff

(Moopam, 1998)

(NO2)

Grassoff

(Moopam, 1998)

(NH3)

Korolaff

(Moopam, 1998) nm

(PO4)

Korolaff nm

(Moopam, 1998)

Korolaff nm

(Moopam, 1998)

(Biological Oxygen Demand)BOD5

(CAMLAB) BOD5 BOD5

TCBS(Thiosolphate Citrate Bile Salt) Agar)

SDA(SABouraud Dextrose Agar)

OIE WSSV TSV IHHNV Biocheep

EXCEL

SPSS PP-PLOT

one-way ANOVA

T-Test

T3 T2 (P<0.05

T1 P>0.05

P<0.05

P>0.05

T3 T2 P<0.05

T1 P>0.05

T3 P<0.05

P<0.05

P>0.05 T3 T2

P<0.05 T1

T2 P<0.05

T1 P>0.05 T3

T3 P<0.05

T3 T2 P<0.05

T1 P>0.05

T3 P<0.05

P<0.05

P>0.05 T3 T2

P<0.05 T1

P>0.05

T1 T2 T3

a a a a a a a b a ab ab b

a b b b b b

T2 T3 T1

T2 T3

P>0.05

Biomass

(P<0.05)

T2 P>0.05 T3 T2

T3 P>0.05 T1

T3 P<0.05 T1

T1 FCR T3 FCR

(P<0.05)

T3 P>0.05 T1 T2

P<0.05

PH T1 PH

P<0.05

T1 P>0.05 T3 T2

P<0.05

b b

PH T-Test

PH PH T-Test P<0.05

P<0.05

PH PH T-Test

P<0.05

T3 T2 P<0.05

P<0.05 T1 P>0.05

T-Test

T-Test P<0.05

P<0.05

T-Test

P<0.05

P>0.05

T-Test

P<0.05

T-Test P>0.05

P<0.05

b b b a a a

(NO2)

P<0.05 NO2

T3 T2 NO2

P<0.05 T1 P>0.05

(NO3)

P>0.05 NO3

(NH3)

P<0.05 NH3

T3 T2 NH3

P<0.05 T1 P>0.05

T3 T2 T1

(p>0.05)

(PO4)

T3 T2 T1

(p>0.05)

(Biological Oxygen Demand)BOD5

BOD5

P<0.05 BOD5

P>0.05 T3 T2 BOD5

P<0.05 T1

BOD5

(TOM)

TOM

P<0.05 TOM

P>0.05 T3 T2 TOM

P<0.05 T1

TOM

P>0.05 a

Dinophyceae Cyanophyceae Bacillariophyceae

Gyrosigma sp. Pridinium sp. Amphora sp. Ceratium sp. Licmophora sp. Prorocentrum sp. Chaetoceros sp. Melosira sp. Pyrophacus sp. Chattonella sp. Nevicula sp. Rhizosolenia sp. Cocinodiscus sp. Nitzschia sp. Scrippsiella sp. Coconeis sp. Odentella sp Sorella sp. Dictyoca sp. Oscillatoria sp. Synedra sp. Diploneis sp. Phormidium sp. Talasionema sp. Gymnodinim sp. Pleurosigma sp. unknown-1

Nitzschia sp. Nevicula sp.

Oscillatoria sp. Pridinium sp. Melosira sp. Pleurosigma sp.

Nitzschia sp. Nevicula sp.

Mollusca Copepoda Nauplli

Tintinnida Annelida Sarcomastigophora

Phylum: Protozoa Class: Ciliata Family:Tintinidae(Favella sp., Tintinopsis sp), Class:Rizopoda Order: Framinifera Phylum: Mollusca Class: Gastropoda, Bivalva Phylum: Annelida Class: Polycheat Phylum: Arthropoda Class: Copepoda Order: Calanoida, Cyclopoida, Harpacticoid , Poecilostomatoids Other: Egg Brachionus , Brachionus ,Nematod

T3 T2 T2

P>0.05

T3 T2

Epistylis sp. (Zoothamnium sp

V.nerets V.natrigenes V.splendidus V.alginoliticus

V.comphelli V.fluvialis V.caticula

TCBS

WSSV/IHHNV/TSV PCR

T1 T3 T2

.(Thampy et al., 1988 )

(P.monodon) (Mugil cephalus)

(Phuoc, 2007)

T3 T2

FCR

T3 T2

FCR T3 T1 T2

FCR

(Yap, 2001)

Phuoc (Aldridge & Salazar., 2008)

T3 T2 T1

Phuoc

T3 T2

.(Odum, 1970 & Zismann et al., 1975)

(Thomson, 1963)

(Odum, 1970)

Chein., %

1992

Detritus

(Boyd, 1992)

T1 T3 T2

P>0.05

T3 T2 T1

T3 T2

(Pruginin et al., 1975)

M.cephalus

T3 T2

(p>0.05)

T1 T1 PH

T2 T2 PH

T1 BOD5

BOD5

(Boyd & Tucker, 1998)

Briggs

Wickins & lee, 2002 ; Boyd, 2001

(p>0.05)

Chein, 1992 mg/lit Boyd, 2001

Boyd, 2001 ;

Wickins & lee, 2002

(p<0.05)

Chein, 1992

NH3

NH3 Wickins

ANCAP, 1978

(Jifsan good aquaculture practices manual, 2007)

( Mugil cephalus) (P.monodon)

NH3

(Phuoc, 2007)

(P.indicus)

(P.stylirostris) (P.chinensis) P.monodon)

FCR

(Yap, 2001)

(Tendencia et al.,2006)

Scophthalmus rhombus Pollachius virens Labrus berglata Plathichthys flesus Solea Solea

(Hellio et al., 2002 ; Ebram et al., 1999)

V.Comphelli V.Nartrigenes V.Splendidus V.Nerets V.Costicola V.Fluvialis V.Alginoliticus

V.Fluvialis V.Alginoliticus

T3 T1 (Yap, 2001)

(P.mergaiensis) (P.monodon)

(Chein,

1992)

Biswass, 1992)

T3 T2

T1 T2 T3

T3 T1

T1 T2

T2 T3

T1 T2,T3

T2,T3

T1 T2 T3

Mugil capoito

(Lithopenaeus vannamei)

(Chanos (Penaeus indicus)

chanos)

(Fenerropenaeus indicus)

(Pnaeus indicus)

(Lithopenaeus vannamei)

Lithopenaeus vannamei

(Lithopenaeus vannamei)

1- APHA., 1998. Standard methods for examination of water and waste water. 1-Abdel Tawwab, M. ; Eid, A.H.M. ; Abdelghany, AE and Marakby,H.I.E., 2005. The Assessment of water quality and primary productivity in earthen fishponds stocked with stripped mullet (Mugil cephalus) and subjected to different feeding regimes. Turkish journal of fisheries and Aquatic Sciences (5) :1-10. 2-Aldridge, D.C. and Salazar, J.C, 2008. Effect of different stocking combination on growth, production and survival of milk fish (Chanos chanos forska) and prawn (Penaeus monodon fabricus) in polyculture in brackish water ponds. Aquaculture, Valume 281, 34-42. 3-ANCAP, 1978. ManualonPondcultureof Penaeid Shrimp. ASEAN National cording Agency of the Philippines, 132 p. 3-Apud, F.D. ;Primarva, J.H. and Torres, P.L., 1983. Farming of prawns and shrimps. Southeast Asian Fisheries Developmet Center. Extension Manual, vol. 39, pp. 1- 67. 4-Bardach, J . H ; Ryther ,J. H . ,and Mclarney, W. O . , 1972 . Aquaculture: the farming and husbandry of freshwater and marine organisms. Wiley Interscience, New York, 868 pp. 5-Biswas, K.P. 1992. Prevention and central if fish and prawn disease P: 144. Narendra publishiny house. 6-Boltovskoy,D.,1999.South Atlantic Zooplankton.Backhuys publishers.,Leiden the Netherlands.868pp. 7-Boyd. C., 1992. Water quality management for pond fish culture (4 Thed) Elsevier publishers B. V. Netherlands. Science. 8-Boyd, C. E. and C. S. Tuker, 1998. Pond aquaculture water quality Management. KluwerAcademic publishers, Bostone, Masachusettes, 700p. 9-Boyd, C.E, 2001.Soil and water quality considerations in shrimp farming. Auburn Univ. Alabama, USA. 30P. 10-Briggs, M and Smith, F- S . , 2004. Introduction and movement of Penaeus vannamei and Penaeus stylirostris in Asia and the pacific. FAO. 11-Carmelo, R . 1977.Identifying marine phytoplankton. Acodemic Press.878pp. 12-Chein. Y. H . , 1992. Water quality requirement and management for marine shrimp culture. Technical bulletin.pp.30. 13-Clesceri, S. ; E. Greenberg & R. Trussll, 1989. Standard methods for the examination of water and waste water, APHA (American Public Health Association). Washington, D. C., USA.

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Abstract

This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m2) per m2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m2 2 2 (T1/), 2/100 m (T2) and 4/100 m (T3). During experiment, the physicochemical parameters of water (temperature, O2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P>0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T3 and T1 respectively. Although, there were no significant differences between T2 and T3 in ammonia, O2, Total organic material, pH and BOD5, but, T2 and T3 had significant differences with T1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp. Key words: Litopenaeus vannamei, Mugil cephalus, mixed culturing, Guater and Chabahar.

Ministry of Jihad e Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION Offshore Fisheries Research Center

Title : Investigation on Feasibility Polyculture of Litopneaus vannamei with Mugil cephalus in Earthen Shrimp ponds Apprpved Number: : 2-78-12-88028 Author: Seyed Hossein Hosseini Aghouzini Executor : Seyed Hossein Hosseini Aghouzini Collaborator : M . Hafezieh ; A . Ezhdehakoshpor ; S . A . Mousavi Gelsefid ; B . Azhang ; H . Jahantigh, A . Matinfar ; M . Azhir Advisor(s):- Location of execution : Sistan - o Balouchestan province Date of Beginning : 2009 Period of execution : 1 year & 6 months Publisher : Iranian Fisheries Research Organization Circulation : 20 Date of publishing : 2012 All Right Reserved . No Part of this Publication May be Reproduced or Transmitted without indicating the Original Reference

MINISTRY OF JIHAD - E - AGRICULTURE AGRICULTURAL RESEARCH , EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION Offshore Fisheries Research Center

Titel: Investigation on Feasibility Polyculture of Litopneaus vannamei with Mugilcephalus in Earthen Shrimp ponds

Executor: Seyed Hossein Hosseini Aghouzini

Registration Number 40034