TABLE OF CONTENT • Organizing committees ....................................................................................... p 4 • Oral presentation abstracts................................................................................ p 5 • Poster abstracts................................................................................................ p 75 • Presentation of the society Plant Signaling and Behavior ............................ p 128 • Presentation of the French society for plant biology ..................................... p 129 • Presentation of university Paris-Diderot ....................................................... p 130 • Presentation of the work of Gianluca Balocco “Naked plant” ...................... p 132 • Sponsors ......................................................................................................... p 134 2 EDITORIAL On behalf of the organizing committees it is PSB 2015 takes place for the first time in a great pleasure to welcome you in Paris for Paris. This is an exciting opportunity for the 3rd International Symposium on Plant researchers around the world to gather and Signaling and Behavior. discuss the latest advances, the newest ideas and the potential for creative future research The symposium, held from 29 June to 2 July to understand the ways that plants sense, 2015 on the campus of the University Paris interact, communicate, learn, develop, Diderot-Paris 7, is under the auspices of the survive and grow in an ever-changing world. international society Plant Signaling and We hope it will foster trans-disciplinary Behavior (PSB) and the French Society of interactions among plant biologists, cell Plant Biology (SFBV). biologists, plant ecologists but also physicists and chemists and all researchers interested in the plant field François Bouteau On behalf of the organizing committees web site: http://psb2015.com 3 ORGANIZING COMMittEES LOCAL COMMITTEE SCIENTIFIC COMMITTEE Delphine Bonnin, Chedly Abdelly, Université Paris Diderot University of Tunis François Bouteau, František Baluška, Université Paris Diderot University of Bonn Jean-Marie Frachisse, Satish C Bhatla, CNRS, Gif sur Yvette University of Dheli Bertrand Gakière, François Bouteau, Université Paris Sud Université Paris Diderot Eric Herbert, Rafik Errakhi, Université Paris Diderot Eléphant Vert Patrice Meimoun, Jean marie Frachisse, UPMC CNRS, Gif sur Yvette Patrice Thuleau, Bertrand Gakière, CNRS Toulouse and Université Paul Sabatier Université Paris Sud Eric Herbert, SUPPOrt IN LOCAL ORGANIZATION Université Paris Diderot Tomonori Kawano, Ibtissem Ben Hamed, University of Kitakyushu Université Paris Diderot Arnaud Lehner, Thimoty Tatéossian, Université de Rouen Rice University Anis Limami, Kota Ueda, Université d’Angers University of Kitakyushu Stefano Mancuso, Yuta Okamoto, University of Florence University of Kitakyushu Patrice Meimoun, UPMC Axel Mithoefer, Max-Planck-Institute, Jena Patrice Thuleau, CNRS Toulouse and Université Paul Sabatier Liz Van Volkenburgh, University of Washington Ken Yokawa, University of Bonn Velemir Ninkovic, Swedish University of Agricultural Sciences 4 © Gianluca Balocco Oral presentation abstracts 5 SESSION 1 PLANT BEHAviOR Chairman Stefano Mancuso Understanding plant behavior and highlighting the structure of the information network that exists within plants is one of the main goal of the PSB society. This session will present some of the most recent advances in understanding how and what plants can perceive in their environment to adapt their behavior and discuss how such data can give rise to controversy over plant intelligence 6 Abstract 1.1. CLAUDE BERNArd AND PLANT ANAESthESIA Baluška F1, Yokawa K1, Mancuso S2 Claude Bernard (1813-1878) is famous for References introducing rigorous experimental methods Baluška F, Mancuso S, Volkmann D, Barlow to both medicine and biology. He was one PW (2012) The ‘root-brain’ hypothesis of of the first system biologist who considered Charles and Francis Darwin. Revival after organisms for integrated networks of more than 125 years. Plant Signal Behav 4: hierarchical systems actively maintaining 1121-1127 the constancy of their internal environment. Bernard C (1878) Lectures on the Phenomena But less known is that he followed steps of of Life Common to Animals and Plants. Alexander von Humboldt in his notion that Charles C Thomas Pub Ltd (June 1974) plants, similarly as animals, are also living De Luccia TP (2012) Mimosa pudica, Dionaea systems endowed with sensitivity to and muscipula and anesthetics. Plant Signal activity towards their external environments. Behav 7: 1163-1167 In order to support this unity of animals Grémiaux A, Yokawa K, Mancuso S, and plants, he was performing a number Baluška F (2014) Plant anesthesia supports of experiments documenting sensitivity of similarities between animals and plants: plants towards the anaesthetics of his time, Claude Bernard’s forgotten studies. Plant such as ether and chloroform. It is not Signal Behav 9: e27886 well known, but the plant stress hormone Rinaldi A (2014) Reawakening anaesthesia ethylene is powerfull anaesthetic. Stressed research. EMBO Rep 15: 1113-1118 or wounded plants produce besides ethylene von Humboldt A (1797) Versuche über die also ether. Moreover, our recent results show gereizte Muskel- und Nervenfaser nebst that plants are sensitive to both general and Vermuthungen über den chemischen Process local anaesthetics. Even the chemically des Lebens in der Thier und Pflanzenwelt. inert, monoatomic and generally unreactive Posen (Let Me Print, September 2012) noble gas xenon, which is excellent anaesthetic too, exerts anaesthetic actions 1/ Department of Agrifood and on plants. Intriguingly, local root anaesthesis Environmental of Mimosa plants causes reversible loss of Science, University of Florence, Italy leaf responsivity to mechanical stimuli. This 2/ IZMB, University of Bonn, Germany suprising finding suggest that the plant E-mail: [email protected] bodies are not only highly integrated but that root anaesthesia is switching off the active shoot behaviour. This finding is supporting the ‘root-brain’ hypothesis of Charles and Francis Darwin. 7 Abstract 1.2. AirBORNE PLANT-PLANT SIGNALING LEADS TO A priMING EFFECT ON SALINity TOLERANCE IN VICIA FABA L Caparrotta S, Boni S, Lanza M, Taiti C, Pandolfi C and Mancuso S Plants are exposed to many environmental We confirmed a significant reduction stresses and adjust their physiological state of photosynthetic rate and stomatal in response to these factors. Being sessile conductance as well as high accumulation of organisms, plants evolved sophisticated Na+ in the stressed-emitters. Concurrently, mechanisms to transmit signals not only the relative growth rate (RGR) in the receivers within but also between plants. For example, was interestingly reduced compared to the the defence mechanisms to biotic stresses, control. After two weeks of treatment, also such as herbivores or pathogens, induce the receivers were stressed with a solution the release of volatile organic compounds of NaCl, in order to investigate a possible (VOCs) which are able to elicit a response effect of priming of the airborne signals on in neighbouring plants. Although the neighbouring plants. After stress RGRs of communication of biotic stress between primed plants didn’t change demonstrating plants is relatively well understood, very few that such plants were able to cope with the studies deal with abiotic stresses. In order to stress better than the controls. observe the communication of abiotic stress Taken together our results suggest that among plants, we investigated whether VOCs produced by salt stressed plants VOC emission induced by salt stress in Vicia trigger some form of priming mechanisms faba plants (the emitters) were able to alert on the receivers, leading to an enhanced salt neighbouring plants (the receivers) and elicit tolerance. a stress response and/or an induction of the resistance to the stress itself. We applied Department of Agrifood Production and a mild salinity stress to the emitters and Environmental Sciences, University of we monitored the physiology of the plants Florence, for two weeks. The receivers were located Sesto F.no, Florence – 50019, Italy in close proximity and shared the same E-mail: [email protected] environment with the stressed plants. At the same time unstressed plants, grown in a similar environment, were used as control. 8 Abstract 1.3. ACOUStiC SENSING OF BELOWGROUND PLANT GROWth AND MONitOriNG OF FRUit ripENING Comparini D1,2, Iwase J3, Masi E4, Mancuso S2,4, Sato Y3, and Kawano T1,2,5 Observation of roots, bulbs and tubers 1 Faculty of Environmental Engineering growing belowground is largely limited & International Photosynthesis by the difficulty to monitor root growth Industrialization Research Center, The and activity without removal of the soil. University of Kitakyushu, Kitakyushu 808- Dynamic growth and development of intact 0135, Japan. root systems or crops of interest are hardly 2 University of Florence LINV Kitakyushu monitored since plant organs growing in the Research Center (LINV@Kitakyushu), soil cannot be detected or visualized by any Kitakyushu 808-0134, Japan. conventional optical instruments. For this 3 Collaboration center, Kyushu Institute of reason, non-invasive methods for detection Technology, Kitakyushu 808-0196, Japan. of sub-terrestrial objects have been recently 4 LINV-DiSPAA, Department of Agri-Food attracted the attentions by researchers who and