Quinine Production by Endophytic Cercospora Spp. (Fungi, Mycosphaerellaceae) from Cinchona Calisaya (Rubiaceae)

Total Page：16

File Type：pdf, Size：1020Kb

Quinine Production by Endophytic Cercospora Spp. (Fungi, Mycosphaerellaceae) from Cinchona Calisaya (Rubiaceae)

AMC 2019 Poster Presentation 4 P4-33 Quinine Production by Endophytic Cercospora spp. (Fungi, Mycosphaerellaceae) from Cinchona calisaya (Rubiaceae) Iman Hidayat1), Nani Radiastuti2), Izumi Okane3), Suminar Setiati Achmadi4), Gayuh Rahayu5) 1)Research Center for Biology, Indonesian Institute of Sciences (LIPI), Indonesia 2)Faculty of Science and Technology, State Islamic University Syarif Hidayatullah, Indonesia 3)Faculty of Life and Environmental Sciences, University of Tsukuba, Japan 4)Chemistry Department, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB), Indonesia 5)Biology Department, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB), Indonesia Aims: Genus Cercospora has been recognized as a common fungal pathogen causing leaf spot on various plants. During the study of fungal endophytes from C. calisaya in Indonesia, two unsporulated fungal isolates (Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190) resemble the colony characteristics of Cercospora were determined. The current study aimed at identifying the species name of both strains using combination of morphology data and phylogenetic analysis, and analyzing their cinchona alkaloids profile. Methodology and Results: The phylogenetic analysis was carried out based on combination of the internal transcribed spacer (ITS), part of the elongation factor (EF) 1-α, actin (ACT), calmodulin (CAL) and histon (HIS) sequences. While cinchona alkaloids profile was analyzed using HPLC method. The phylogenetic tree generated from multilocus analysis confirmed that both isolates belong to the genus Cercospora, and formed an independent clade separated from other Cercospora sequences. This indicates that both strains belonging to a new taxon, however, further study involving sporulation induction assay is necessary to verify the identity of both isolates. Analysis of cinchona alkaloids using HPLC method showed that quinine was detected from Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190 at concentration 1.2 mg/L and 0.7 mg/L, respectively. Conclusion: Significance and Impact of study: Although Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190 are capable in producing quinine, further fermentation study in optimizing quinine production is necessary for scaling up. Key words: alkaloids, Cinchona, Fungi, Mycosphaerellaceae, phylogeny Asian Mycological Congress 2019.

Copy Link

Recommended publications

Cinchona Amazonica Standl. (Rubiaceae) No Estado Do Acre, Brasil1 Cinchona Amazonica Standl
Bol. Mus. Para. Emílio Goeldi. Ciências Naturais, Belém, v. 1, n. 1, p. 9-18, jan-abr. 2006 Cinchona amazonica Standl. (Rubiaceae) no estado do Acre, Brasil1 Cinchona amazonica Standl. (Rubiaceae) in the state of Acre, Brazil Percy Amilcar Zevallos Pollito I Mário Tomazello Filho II ResumoResumo: O presente estudo trata da dendrologia, distribuição geográfica e status de conservação de Cinchona amazonica Standl., plantas de interesse medicinal que ocorrem no estado do Acre, Brasil. A pesquisa consistiu em trabalhos de campo para a coleta de material botânico, levantamento e estudo das exsicatas disponíveis nos herbários nacionais e internacionais da América do Sul, revisão bibliográfica das espécies do gênero na literatura e nos sites especializados. Os resultados apresentam a descrição com a sua ilustração e mapeamento bem como determinação da sua situação populacional. Palavras-chavealavras-chave: Cinchona. Dendrologia. Ditribuição geográfica. Status de conservação. Acre. AbstractAbstract: The present research is about dendrology, geographic distribution and status of conservation of Cinchona amazonica Standl. with are plants with medical interest; they grow in the state of Acre, Brazil. The research consisted in: field work for the collection of botanical material, in the survey and study of the available exsiccates in the national and international herbariums of the South America. It has been done a bibliographical review of the species of this genera in literature and the specialized sites The results present the description of the species with their illustration and mapping, as well as determination of their population situation. Keywordseywords: Cinchona. Dendrology. Geographic distribution. Conservation status. Acre I Universidade Nacional Agraria La Molina. Faculdad de Ciencias Florestales.
[Show full text]
Plants and Parts of Plants Used in Food Supplements: an Approach to Their
370 ANN IST SUPER SANITÀ 2010 | VOL. 46, NO. 4: 370-388 DOI: 10.4415/ANN_10_04_05 ES I Plants and parts of plants used OLOG D in food supplements: an approach ETHO to their safety assessment M (a) (b) (b) (b) D Brunella Carratù , Elena Federici , Francesca R. Gallo , Andrea Geraci , N (a) (b) (b) (a) A Marco Guidotti , Giuseppina Multari , Giovanna Palazzino and Elisabetta Sanzini (a)Dipartimento di Sanità Pubblica Veterinaria e Sicurezza Alimentare; RCH (b) A Dipartimento del Farmaco, Istituto Superiore di Sanità, Rome, Italy ESE R Summary. In Italy most herbal products are sold as food supplements and are subject only to food law. A list of about 1200 plants authorised for use in food supplements has been compiled by the Italian Ministry of Health. In order to review and possibly improve the Ministry’s list an ad hoc working group of Istituto Superiore di Sanità was requested to provide a technical and scientific opinion on plant safety. The listed plants were evaluated on the basis of their use in food, therapeu- tic activity, human toxicity and in no-alimentary fields. Toxicity was also assessed and plant limita- tions to use in food supplements were defined. Key words: food supplements, botanicals, herbal products, safety assessment. Riassunto (Piante o parti di piante usate negli integratori alimentari: un approccio per la valutazione della loro sicurezza d’uso). In Italia i prodotti a base di piante utilizzati a scopo salutistico sono in- tegratori alimentari e pertanto devono essere commercializzati secondo le normative degli alimenti. Le piante che possono essere impiegate sono raccolte in una “lista di piante ammesse” stabilita dal Ministero della Salute.
[Show full text]
God's Healing Leaves: the Colonial Quest for Medicinal Plants in The
GOD’S HEALING LEAVES: THE COLONIAL QUEST FOR MEDICINAL PLANTS IN THE TORRID ZONE* ROBERT VOEKS and CHARLOTTE GREENE ABSTRACT. The colonial era witnessed a fevered quest for exotic medicinal plants by Eur- opean physicians and scientists. This essay explores the geographical principles that oriented the search towards the lands and peoples of the humid tropics. Believing that God had planted botanical cures for diseases in their places of origin, medicinal plant collectors concentrated their efforts in the pestilential equatorial latitudes. Although many subscribed to the ancient Doctrine of Signatures, colonial bioprospectors discov- ered early that indigenous and diasporic peoples represented storehouses of plant knowl- edge. Assuming that native knowhow constituted more instinct than intelligence, Europeans employed coercion, bribes, torture, and promises of freedom to extract their ethnomedical secrets. In the case of especially lucrative healing plants, imperial and colo- nial entities conspired to pilfer and naturalize endemic species in their distant colonies. In response to this legacy of inappropriate exploitation of native peoples and tropical plants during the colonial era, most present day bioprospectors follow established codes of ethnobotanical ethics. Keywords: Medicine, botany, tropical rainforest. In the late-twentieth century, the public’s attention was drawn to the world’s tropical rainforests, not just as exotic bestiaries and biodiversity hotspots, but as sources of miracle-cure drug plants. Popular magazines and books touted the pharmaceutical potential of rainforest vegetation, citing for example the case of quinine derived from South American cinchona trees, the historical remedy for malaria, and especially the 1970s discovery of vincristine from the Madagascar periwinkle (Catharanthus roseus), the highly-successful therapy for childhood leukemia.
[Show full text]
Cinchona Pubescens Quinine Tree Rubiaceae
Cinchona pubescens Quinine tree Rubiaceae Forest Starr, Kim Starr, and Lloyd Loope United States Geological Survey--Biological Resources Division Haleakala Field Station, Maui, Hawai'i January, 2003 OVERVIEW Cinchona pubescens, native from Andean South America north to Costa Rica, has been cultivated in various tropical regions of the world mainly for use in the production of quinine, a medicine used to treat malaria, which is obtained from the root and bark of the tree. In some places where C. pubescens is cultivated, such as Galapagos and Hawai'i, it is spreading from initial plantings, invading nearby forests, forming dense thickets, and crowding out native plants. In Hawai'i, Cinchona plantations were planted as early as 1868 on Maui with later plantings by state foresters on O'ahu, Maui, and Hawai'i in the first half of the 1900's (Wagner et al. 1999). C. pubescens was reported as naturalized in 1978 from the island of Hawai'i and in 1987 from Maui (Wagner et al. 1999). On Maui, C. pubescens is locally abundant in the Makawao Forest Reserve of East Maui in areas near original plantings and alien forestry plantations, along old roads, and in thick native mixed mesic to wet forests. Though seed production is heavy, this species does not seem to disperse very far from original plants. Though locally abundant, it is fairly far along in its invasion process, and the terrain is very difficult, including steep gulches and thick under-story vegetation, making control of this species difficult. While eradication of C. pubescens in the Makawao Forest Reserve would be difficult, controlling small populations in pockets of rich native vegetation may help contain the infestation and slow the degradation of the remaining native forests in the area.
[Show full text]
Phytogeographic Basis Plant Breeding
PHYTOGEOGRAPHIC BASIS of PLANT BREEDING 1. Local Varieties and Their Significance :— The -varieties of cultivated plants grown in the different regions of the Soviet Union until recently were varieties introduced from various localities and countries, and were inseparable from human migration and colonization. The list of cultivated plants reflects the history of our country in its recent past, it shows the effects of individual peasant farming. In the separate groups and varieties of plants one can trace the routes by which they were brought from Western Europe, the United States, Asia Minor, Mongolia, and Iran. In the pre-revolutionary period, the introduction of new varieties in our country was haphazard. Beginning with the eighteenth century, individual amateur growers and societies unsystemati- cally introduced new varieties from abroad. Sometimes these new varieties were quite valuable but because of the vastness of our country and the com- plete absence of any state-planned system of plant introduction, the imported varieties usually restricted themselves to very limited areas and disappeared. It may be considered that pedigree seed production, in the real meaning of the term, did not exist in our country before the October Revolution. We have just begun a planned distribution of varieties in accordance with the needs of our large-scale socialized and mechanized agricultural economy. Yet, there is no doubt that the varietal materials which were introduced in our country and cultivated for decades and centuries were subjected to natural selection, and also to deliberate or casual artificial selection, and that some local varieties evolved that were ecologically adapted. The proximity of the Soviet Union to the basic centers of origin of numer- ous cultivated plants facilitated the selection of exceptionally valuable forms.
[Show full text]
Phylogeny of the Tribe Cinchoneae (Rubiaceae), Its Position in Cinchonoideae, and Description of a New Genus, Ciliosemina
54 (1) • February 2005: 17–28 Andersson & Antonelli • Phylogeny of Cinchoneae MOLECULAR PHYLOGENETICS Phylogeny of the tribe Cinchoneae (Rubiaceae), its position in Cinchonoideae, and description of a new genus, Ciliosemina Lennart Andersson* & Alexandre Antonelli Botanical Institute, Göteborg University, P. O. Box 461, SE-405 30 Göteborg, Sweden. alexandre.antonelli@ botany.gu.se (author for correspondence) Relationships of and within the Rubiaceae tribe Cinchoneae were estimated based on DNA sequence variation in five loci: the ITS region, the matK and rbcL genes, the rps16 intron, and the trnL-F region including the trnL intron and the trnL-F intergenic spacer. Within Cinchonoideae s.s., the tribe Naucleeae is the sister group of a clade that comprises all other taxa. Cinchoneae and Isertieae s.s., are strongly supported as sister groups. The tribe Cinchoneae is strongly supported as monophyletic in a restricted sense, including the genera Cinchona, Cinchonopsis, Joosia, Ladenbergia, Remijia and Stilpnophyllum. There is strong support that these genera are monophyletic as presently conceived, except that one species mostly referred to Remijia is of uncer- tain phylogenetic affinity. To accommodate this species and a morphologically closely similar one, a new genus, Ciliosemina A. Antonelli, is proposed and two new combinations are made. KEYWORDS: Cinchona, Cinchoneae, Cinchonopsis, Joosia, Ladenbergia, Remijia, Stilpnophyllum, Rubiaceae; ITS, matK, rbcL, rps16 intron, trnL-F. oideae. Bremekamp (e.g., 1966) revised Schumann’s INTRODUCTION classification and redefined Cinchonoideae to comprise Traditionally (e.g., Candolle, 1830; Schumann, only genera without raphides, with imbricate or valvate 1891, 1897; Robbrecht, 1988), the tribe Cinchoneae has corolla aestivation and testa cells with coarsely pitted been circumscribed to include about 50 genera with basal walls.
[Show full text]
Cinchona Or the Peruvian Bark
HISTORY PLANTS AGAINST MALARIA PART 1: CINCHONA OR THE PERUVIAN BARK M.R. Lee, Emeritus Professor of Clinical Pharmacology and Therapeutics, University of Edinburgh One of the most compelling sagas in the history of were prepared to use their knowledge of the bark to aid medicine and therapeutics is the emergence of the their struggles for power within the Church! Peruvian bark (Cinchona) and also of the pharma- cologically active substance derived from it, quinine. Its An important ecclesiastical figure, Cardinal de Lugo, now discovery involved exploration, exploitation and secrecy, emerges to take the initiative in the history of Cinchona and it came, in the nineteenth century, to reflect the (Figure 1). De Lugo was born in Madrid in 1585 but struggles of the major European powers for domination, spent his early years in Seville where he later became a territory and profit. This short history shows how the Jesuit priest.4 In 1621 he was transferred to the Collegium use of Cinchona enabled the exploration of dangerous Romanum in Rome, then the leading educational malarial areas and in this way facilitated imperial institution of the Jesuits. He taught there with distinction expansion by the Western powers. from his arrival until 1643. Initially de Lugo probably obtained the Peruvian bark from Father Tafur S.J. and, impressed by his preliminary trials of its efficacy, he THE PERUVIAN; JESUIT’S OR CARDINAL’S BARK purchased large amounts of it at his own expense. Many physicians are familiar with the story of the Countess Sturmius, a contemporary, reported that de Lugo ‘gave it of Chinchón, wife of the Viceroy of Peru; it was claimed gratis to the fevered poor, on condition only, that they by Bado that she was cured of a certain ague by did not sell it and that they presented a physician’s Cinchona bark sometime in the late 1620s or early statement about the illness’.
[Show full text]
Download This PDF File
REINWARDTIA Published by Herbarium Bogoriense - LBN, Bogor Vol. 10, Part 2, pp. 245 - 264 (1985) HASSKARL'S CINCHONA BARKS 1. HISTORICAL REVIEW C. E. RIDSDALE Rijksherbarium, Leiden, Netherlands & L. A. ANDERSON. A. T. KEENE & J. I). PHILLIPSON Department of Pharmacognosy, The School of Pharmacy, University of London, U.K. ABSTRACT The preliminary results of alkaloid analyses of Hasskaii's cinchona bark collection, made in Peru in 1852/3' is given, and the identity and historical aspects of the material discussed in respect of the role the plants played in the Javanese plantations. ABSTRAK Hasil sementara analisis alkaloid koleksi pepagan kina Hasskarl yang dibuat di Peru tahun 1852/1853 disajikan. Identitas dan segi sejarah bahan tadi dibahas dalam kaitannya dengan peranan tanaman di perkebunan di pulau Jawa. INTRODUCTION The Dutch were the first to successfully organize Cinchona planta- tions, which were situated in Java. Materials for these plantations were obtained over a number of years by the import of plants or seeds from various sources either direct, e.g. by Hasskarl, Ledger, Karstens and Schuhkraft, or later indirect through exchange of materials from former British India. The basis of the first planting stocks of Cinchona in the former Dutch East Indies was derived from the plants and seeds collected by Hasskarl on his expedition to S. America in 1852/3. In addition a limited quantity of 'C. calisaya javanica' was propagated from plants produced from cuttings of the first Cinchona plant introduced into Java, which was obtained by De Vriese (De Vriese 1885) from Messers Thibant & Keteleer in Paris, an offspring from seed collected by Weddell.
[Show full text]
Pharmacognosy of Cinchona
www.ijcrt.org © 2020 IJCRT | Volume 8, Issue 12 December 2020 | ISSN: 2320-2882 Pharmacognosy of cinchona Vishal Prajapati, Pyiush Yadav, Ajeet Kumar Kannaujiya, Ravikant Vishwkarma, Akshay Pratap Yadav Department of pharmacy, Prasad Institute of Technology, Jaunpur (UP) , India ABSTRACT The near about 350,000 species of plants are found in the earth and these plants have the great medicinal value. The plant of Cinchona is also known as Jesuit’s bark, Peruvian bark and cinchona bark which is belong from the family of Rubiaceae. It produces the yellowish to green colored in short ruptur to outer surface and which is inner surface is yellow, brown, deep reddish to brown colored, different- 2 species inner surface of color is different.The cinchona bark are the consist of 30 types of alkaloids, which belong to quinoline groupbut mainly quinidine , quinine, cinchonina and cinchonidine are the play the vital role in medicinal field . Quinine are mainly used to treatment of malaria and other alkaloids are producing the various medicinal properties such as analgesic, antipyretic, protoplasmic, cardiac arrhythmia, dyspepsia ,gastric catarrh and cardiac depressant . KEYWORDS Introduction , Biological source, Geographical source, Botanical classification, Organoleptic property, Microscopic property, Cultivation and collection, Chemical consitituents, Pharmaceutical uses INTRODUCTION The cinchona is a larger shrub or small tree is domestic or born to South America. Cinchona bark is also known as Peruvian bark or Jesuit’s bark , it is famous his medicinal
[Show full text]
Alkaloids – Secrets of Life
ALKALOIDS – SECRETS OF LIFE ALKALOID CHEMISTRY, BIOLOGICAL SIGNIFICANCE, APPLICATIONS AND ECOLOGICAL ROLE This page intentionally left blank ALKALOIDS – SECRETS OF LIFE ALKALOID CHEMISTRY, BIOLOGICAL SIGNIFICANCE, APPLICATIONS AND ECOLOGICAL ROLE Tadeusz Aniszewski Associate Professor in Applied Botany Senior Lecturer Research and Teaching Laboratory of Applied Botany Faculty of Biosciences University of Joensuu Joensuu Finland Amsterdam • Boston • Heidelberg • London • New York • Oxford • Paris San Diego • San Francisco • Singapore • Sydney • Tokyo Elsevier Radarweg 29, PO Box 211, 1000 AE Amsterdam, The Netherlands The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, UK First edition 2007 Copyright © 2007 Elsevier B.V. All rights reserved No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, mechanical, photocopying, recording or otherwise without the prior written permission of the publisher Permissions may be sought directly from Elsevier’s Science & Technology Rights Department in Oxford, UK: phone (+44) (0) 1865 843830; fax (+44) (0) 1865 853333; email: [email protected]. Alternatively you can submit your request online by visiting the Elsevier web site at http://elsevier.com/locate/permissions, and selecting Obtaining permission to use Elsevier material Notice No responsibility is assumed by the publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation
[Show full text]
Medicinal Plants of Brazil Introduction
www.asiabiotech.com Special Feature Dr Robin Mitra1, Associate Professor Brad Mitchell2, Professor Chris Gray3, Professor John Orbell4, Dr Tony Coulepis5 and Dr Morley Somasundaram Muralitharan6 Medicinal Plants of Brazil Introduction Brazil is the largest country in Latin America covering a territory of 8,514,876.599 km² that lies between central South America and the Atlantic Ocean. Brazil is named after the plant pau-brasil (Caesalpinia echinata L.), a member of the Caesalpiniaceae family which was used in the past to obtain a dye-stuff known as “brasiline” (Goncalves De Lima et al., 1961). Due to Brazil’s varied geographical environment ranging from the Amazonian rainforests to the dry semi-arid hinterland known to the Indians as the “Caatinga” (Anon1 1978), the country is botanically rich with 40 000–60 000 vegetal species (Jorge et al., 2004). The five major biomes of Brazil include the Amazon rain forests that cloak an area larger than western Europe, Cerrado brushlands the home of the Krahô Indians, the Mata Atlântica rain forest, the Pantanal wetlands and the Caatinga semi arid desert (Giorgetti et al., 2007). With the exception of the Amazon forests, there is a huge paucity of documented studies on the medicinal plants concerning other biomes such as the Mata Atlantica, the Pantanal, (Di Stasi et al., 2002) and especially the Caatinga and the Cerrado (Almeida et al., 2006). Historians believe that it was the richness of the flora and the highly prized wood from the forests of Brazil that had played a central role in enticing the early explorers to its shore (De Mello 1980).
[Show full text]
Rubiaceae: Cinchona Calisaya)
Phylogeny predicts the quantity of antimalarial alkaloids within the iconic yellow Cinchona bark (Rubiaceae: Cinchona calisaya) Maldonado Goyzueta, Carla Brenda; Barnes, Christopher James; Cornett, Claus; Holmfred, Else Skovgaard; Hansen, Steen Honoré; Persson, Claes; Antonelli, Alexandre; Rønsted, Nina Published in: Frontiers in Plant Science DOI: 10.3389/fpls.2017.00391 Publication date: 2017 Document version Publisher's PDF, also known as Version of record Document license: CC BY Citation for published version (APA): Maldonado Goyzueta, C. B., Barnes, C. J., Cornett, C., Holmfred, E. S., Hansen, S. H., Persson, C., ... Rønsted, N. (2017). Phylogeny predicts the quantity of antimalarial alkaloids within the iconic yellow Cinchona bark (Rubiaceae: Cinchona calisaya). Frontiers in Plant Science, 8, [391]. https://doi.org/10.3389/fpls.2017.00391 Download date: 08. Apr. 2020 ORIGINAL RESEARCH published: 22 March 2017 doi: 10.3389/fpls.2017.00391 Phylogeny Predicts the Quantity of Antimalarial Alkaloids within the Iconic Yellow Cinchona Bark (Rubiaceae: Cinchona calisaya) Carla Maldonado 1, 2 †, Christopher J. Barnes 1 †, Claus Cornett 3, Else Holmfred 3, Steen H. Hansen 3, Claes Persson 4, 5, Alexandre Antonelli 4, 6 and Nina Rønsted 1* 1 Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark, 2 Herbario Nacional de Bolivia, Universidad Mayor de San Andres, La Paz, Bolivia, 3 Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark, 4 Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden, 5 Gothenburg Global Biodiversity Centre, Gothenburg, Sweden, 6 Gothenburg Botanical Garden, Gothenburg, Sweden Edited by: Danièle Werck, Centre National de la Recherche Considerable inter- and intraspeciﬁc variation with respect to the quantity and Scientiﬁque, France composition of plant natural products exists.
[Show full text]