AMC 2019 Poster Presentation 4 P4-33 Quinine Production by Endophytic Cercospora spp. (Fungi, Mycosphaerellaceae) from Cinchona calisaya (Rubiaceae) Iman Hidayat1), Nani Radiastuti2), Izumi Okane3), Suminar Setiati Achmadi4), Gayuh Rahayu5) 1)Research Center for Biology, Indonesian Institute of Sciences (LIPI), Indonesia 2)Faculty of Science and Technology, State Islamic University Syarif Hidayatullah, Indonesia 3)Faculty of Life and Environmental Sciences, University of Tsukuba, Japan 4)Chemistry Department, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB), Indonesia 5)Biology Department, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB), Indonesia Aims: Genus Cercospora has been recognized as a common fungal pathogen causing leaf spot on various plants. During the study of fungal endophytes from C. calisaya in Indonesia, two unsporulated fungal isolates (Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190) resemble the colony characteristics of Cercospora were determined. The current study aimed at identifying the species name of both strains using combination of morphology data and phylogenetic analysis, and analyzing their cinchona alkaloids profile. Methodology and Results: The phylogenetic analysis was carried out based on combination of the internal transcribed spacer (ITS), part of the elongation factor (EF) 1-α, actin (ACT), calmodulin (CAL) and histon (HIS) sequences. While cinchona alkaloids profile was analyzed using HPLC method. The phylogenetic tree generated from multilocus analysis confirmed that both isolates belong to the genus Cercospora, and formed an independent clade separated from other Cercospora sequences. This indicates that both strains belonging to a new taxon, however, further study involving sporulation induction assay is necessary to verify the identity of both isolates. Analysis of cinchona alkaloids using HPLC method showed that quinine was detected from Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190 at concentration 1.2 mg/L and 0.7 mg/L, respectively. Conclusion: Significance and Impact of study: Although Cercospora sp. strain IPBCC 14.1189 and strain IPBCC 14.1190 are capable in producing quinine, further fermentation study in optimizing quinine production is necessary for scaling up. Key words: alkaloids, Cinchona, Fungi, Mycosphaerellaceae, phylogeny Asian Mycological Congress 2019.